How to grow shiitake mushrooms containing vanadium

Abstract

The present invention relates to a method for cultivating shiitake mushrooms containing vanadium, and more specifically, to a method for cultivating shiitake mushrooms containing vanadium that can increase the vanadium content in high-temperature, medium-temperature, and low-temperature shiitake mushrooms by adjusting the weight of the substrate during the needle-injection process to reach a reference value, and by supplying through humidification when humidity is improved during the process of controlling humidity during the growth process for harvesting. The present invention is a method for cultivating shiitake mushrooms, a) a step of preparing a culture solution by ionizing vanadium and selenium in water; b) A culture medium granulation stage in which outer shell removal, washing, and granulation are performed; c) A step of germinating the culture medium obtained from step b) above for 3 to 5 days; d) A step of performing acupuncture by supplying the culture solution obtained from step a) so that the weight of the medium is maintained at 70–90% of the reference value based on 3 kg; e) A step of thinning out shiitake mushrooms, leaving 8 per substrate depending on their germination status; f) A step for growing ordinary shiitake mushrooms and white shiitake mushrooms; perform, The above general shiitake mushrooms are divided into high-temperature, medium-temperature, and low-temperature types to control the temperature, and The above-mentioned Baekhwago performs a process of raising the temperature and lowering the humidity, and repeatedly lowering the raised temperature and raising the humidity, and a step of spraying a culture solution during humidification while raising the humidity; g) A step of harvesting grown shiitake mushrooms; is included.

Description

Technology Field

[0001] The present invention relates to a method for cultivating shiitake mushrooms containing vanadium, and more specifically, to a method for cultivating shiitake mushrooms containing vanadium that can increase the vanadium content in high-temperature, medium-temperature, and low-temperature shiitake mushrooms by adjusting the weight of the substrate during the needle-injection process to reach a reference value, and by supplying through humidification when humidity is improved during the process of controlling humidity during the growth process for harvesting. Background Technology

[0003] Diabetes can be broadly divided into type 1 diabetes and type 2 diabetes depending on the mechanism of development.

[0004] The aforementioned Type 1 diabetes (insulin-dependent diabetes melitus) occurs due to abnormalities in insulin secretion from the pancreas. The cause is that the beta cells of the pancreas that produce insulin are destroyed by an autoimmune reaction, leading to a decrease in insulin secretion. In contrast, Type 2 diabetes occurs when insulin secretion from beta cells is normal, but insulin resistance in peripheral tissues is high, causing abnormalities in glucose metabolism. It is also known as adult-onset diabetes and accounts for approximately 90% of all diabetes patients.

[0005] If insulin resistance increases due to causes such as obesity, insulin secretion continues to increase, leading to complications, and ultimately, the pancreatic cells that produce insulin lose their function.

[0006] In the progression to type 2 diabetes, there is a stage called prediabetes, which is a term indicating the intermediate stage between normal and diabetes, referring to impaired glucose tolerance and impaired fasting glucose.

[0007] The above-mentioned impaired glucose tolerance refers to a plasma glucose concentration of 141–199 mg / dL 2 hours after an oral glucose tolerance test, and the above-mentioned impaired fasting glucose refers to a fasting plasma glucose concentration of 111–125 mg / dL.

[0008] Prediabetes is a pre-diabetic condition that is a risk factor for type 2 diabetes and cardiovascular disease, and its prevalence is increasing. However, to date, exercise and dietary therapy are recommended instead of specific treatments due to the risk of hypoglycemia that may occur with oral hypoglycemic agents or insulin therapy.

[0009] Consequently, there is a need to develop materials that can prevent diabetes before it develops or prevent its treatment after it occurs.

[0010] One of the dietary therapies involves the consumption of foods containing vanadium. Research on vanadium began about 30 years ago when Professor Yoram Shechter of the Weizmann Institute of Science in Israel discovered through tissue experiments that vanadium, a trace metal element, performs functions similar to the metabolic effects of insulin. He also confirmed that administering vanadium to diabetes can cure various metabolic defects caused by the disease. Furthermore, it was determined that vanadium improves diabetic conditions, based on research results from several joint research teams in Japan showing that natural water containing vanadium, a type of mineral, has an effect on improving diabetes.

[0011] In addition to this, as part of research to utilize vanadium,

[0012] A VANADIUM ION-CONTAINING SUGAR, LIPID AND / OR NITROGEN METABOLIC DISEASE-IMPROVING AGENT (Japanese Patent Publication No.: 2007-031282, (Application Date: Nov. 19, 2003)) is a technology that provides a treatment for the improvement of sugar, nitrogen, and / or lipid metabolic diseases, including diabetes, or diseases related to lipid metabolism or nitrogen metabolism, characterized by being a beverage containing at least 80 μg / L of vanadium ions (V), wherein the vanadium ion (V) content is preferably 100 to 300 μg / L and more preferably 200 to 250 μg / L, and as one embodiment of the present invention, the technology is capable of utilizing the beverage in the form of mineral water, green tea, black tea, juice, or probiotic beverage, etc., and

[0013] VANADIUM ION-CONTAINING AGENT FOR AMELIORATING HYPERTENSION, HYPERLIPEMIA AND NEUTRAL-FAT LIPEMIA AND BLOOD SUGAR-LOWERING AGENT FOR HYPERLIPEMIA AND NEUTRAL-FAT LIPEMIA PATIENT (Japanese Patent Publication No.: 2006-232679, (Application Date: Feb. 22, 2005)) is a technology that verifies the effects of drinking vanadium ion water for a certain period on people with adult diseases and, based on the treatment results thereof, provides a use of vanadium ion water that generates confidence in adult diseases. It is characterized by providing an antihypertensive agent used for the prevention or treatment of hypertension in mammals, and a blood sugar-lowering agent used for the prevention or treatment of diabetes, in the form of an aqueous solution containing vanadium ions (V) at a concentration of at least 80 μg / L.

[0014] The "METHOD FOR PRODUCING VANADIUM-CONTAINING MYCELIUM OF AGARICUS AND FOOD HAVING AGARICUS FUNCTION" (Japanese Patent Publication No.: 2006-180716, (Application Date: Dec. 24, 2004)) provides a functional food containing vanadium, an element known to be effective in preventing diabetes, in Agaricus mushrooms, which are known to possess anti-tumor effects, and a production method for efficiently producing said functional food. The technology involves inoculating a seed culture solution of Agaricus mushroom mycelium into a culture medium containing a water-soluble vanadium compound along with culture components such as a carbon source, a nitrogen source, a mineral source, and water, and culturing it to obtain vanadium-containing Agaricus mushroom mycelium. This Agaricus mushroom mycelium is then separated from the liquid using a separation device such as a centrifuge, dried in a dryer, ground using a grinding device, and then produced as vanadium-containing mycelium powder. Technology characterized by obtaining a functional food that is composed, and

[0015] VANADIUM-CONTAINING RADISH SPROUT AND METHOD FOR CULTURING THE SAME (Japanese Patent Publication No.: 2006-166833) provides a young cotyledon radish containing vanadium, which is said to be an element effective in preventing diabetes, etc., and can be easily prepared as a food for daily use. The invention provides a method for cultivating young cotyledon radish by placing germinated seeds of young cotyledon radish into a hydroponic tank and supplying a liquid fertilizer at a predetermined temperature containing soluble potassium, phosphorus, nitrogen, etc., in the water within the tank. In this method for cultivating young cotyledon radish, it is preferable to set the vanadium concentration in the liquid fertilizer to 0.05 to 10 mg / ℓ, and it is preferable to set the vanadium content of the young cotyledon radish produced according to this method to approximately 10 to 100 ppm.

[0016] As mentioned above, research on the production of materials and products using vanadium is being conducted, but systematic research is still at a minimal level. This technology is also a technology for producing functional materials that can reduce the incidence of diabetes through prevention and play an auxiliary therapeutic role.

[0018] Vanadium is an element with atomic number 23 and symbol V; it is a silvery-gray transition metal belonging to Group 5 (Group 5B) of the periodic table. Vanadium inhibits cholesterol synthesis in the human body, lowers lipid metabolism and blood sugar levels, and makes skin and hair smooth and shiny. Furthermore, it facilitates cardiovascular function and metabolism, and is reported to improve diabetes and hematopoietic function, particularly by promoting insulin secretion (Korean Patent Registration No. 10-2015-0067816).

[0019] Meanwhile, selenium has atomic number 34, symbol Se, belongs to the oxygen group (Group 16, 6A), and is one of the chalcogen elements. Its known effects include regulating thyroid function as a cofactor for thyroid hormone deiodinase, preventing and treating cancer, alleviating AIDS symptoms, suppressing type 2 diabetes, treating or inhibiting the onset of Kasin-back disease, a soilborne disease in Keshan Village, Heilongjiang Province, China, and inhibiting or having anticancer effects against lung, prostate, and colorectal cancers (Korean Patent Registration No. 10-2012-0021105).

[0020] It is known that it is preferable to ingest vanadium and selenium indirectly into the human body in the form contained in plants, rather than directly consuming the components; to this end, much research is being conducted to obtain plants containing vanadium and selenium. However, generally, research is only being conducted on methods to cultivate plants containing only vanadium or selenium, and no method has yet been reported to produce plants with high concentrations or high content of both vanadium and selenium simultaneously. This is because there are many difficulties in finding the optimal ratio of component content in the culture solution used for plant cultivation when producing plants with high concentrations or high content.

[0021] Therefore, there is a growing need for novel methods for the cultivation and production of plants with high concentrations or high content. Prior art literature

[0023] (KR) Patent Registration No. 853205 (Registered Aug. 13, 2008) (JP) Patent Publication No. 2007-031282 (JP) Patent Publication No. 2006-232679 (JP) Patent Publication No. 2006-180716 (JP) Patent Publication No. 2006-166833 The problem to be solved

[0024] The inventors of the present invention have made efforts to develop a method for cultivating shiitake mushrooms with a high concentration or increased content of vanadium and selenium, and as a result, have completed the present invention by confirming that shiitake mushrooms with increased vanadium and selenium content can be obtained when cultivating shiitake mushrooms using a solution in which an aqueous solution of vanadium and an aqueous solution of selenium are mixed in a predetermined ratio.

[0025] Accordingly, the objective of the present invention is to provide a method for cultivating shiitake mushrooms containing vanadium, comprising the steps of: periodically injecting a culture solution containing 17 to 23 ppm of vanadium aqueous solution with respect to water and containing selenium aqueous solution in the same content ratio with respect to the vanadium aqueous solution, such that the volume is adjusted to 75 to 95% of the weight of 3 kg during the needle-injection process of shiitake mushrooms; supplying the culture solution to perform thinning work according to the germination state of the shiitake mushrooms; and supplying the solution during humidification according to the condition of the shiitake mushrooms while raising the humidity to a target value while varying the high, medium, and low temperatures according to the variety of shiitake mushrooms.

[0026] Another objective of the present invention is to provide shiitake mushrooms cultivated by the above-described method for cultivating shiitake mushrooms.

[0027] Other objects and advantages of the present invention will become more apparent from the following detailed description of the invention, claims, and drawings. means of solving the problem

[0029] The present invention is a method for cultivating shiitake mushrooms, wherein

[0030] a) a step of preparing a culture solution by ionizing vanadium and selenium in water;

[0031] b) A culture medium granulation stage in which outer shell removal, washing, and granulation are performed;

[0032] c) A step of germinating the culture medium obtained from step b) above for 3 to 5 days;

[0033] d) A step of performing acupuncture by supplying the culture solution obtained from step a) so that the weight of the medium is maintained at 70–90% of the reference value based on 3 kg;

[0034] e) A step of thinning out shiitake mushrooms, leaving 8 per substrate depending on their germination status;

[0035] f) A step for growing ordinary shiitake mushrooms and white shiitake mushrooms; perform,

[0036] The above general shiitake mushrooms are divided into high-temperature, medium-temperature, and low-temperature types to control the temperature, and

[0037] The above-mentioned Baekhwago performs a process of raising the temperature and lowering the humidity, and repeatedly lowering the raised temperature and raising the humidity, and a step of spraying a culture solution during humidification while raising the humidity;

[0038] g) A step of harvesting grown shiitake mushrooms; is included.

[0040] Step a) above includes preparing a culture solution using 16 to 25 ppm of a vanadium aqueous solution (100 ml of water + 100% pure vanadium) in a water tank supplied with water.

[0042] The above step f) includes controlling the temperature of general shiitake mushrooms by dividing them into high temperature (20–25°C), medium temperature (15–20°C), and low temperature (8–15°C), and spraying a culture solution to grow them when the humidity is controlled to 80–85%.

[0044] Step f) above includes repeating the process of raising the temperature of the Baekhwago from 6 ℃ to 28 ℃ and lowering the humidity from 95% to 50%, lowering the raised temperature from 28 ℃ to 6 ℃ and raising the humidity from 50% to 95%, and repeating the process of controlling the humidity by spraying a culture solution during the process of raising the humidity every 2 hours. Effects of the invention

[0046] The present invention enables the mass production of shiitake mushrooms (including white shiitake) with increased vanadium and selenium content when cultivating shiitake mushrooms using a culture solution in which an aqueous solution of vanadium and an aqueous solution of selenium of a specific concentration are mixed in equal weight ratios.

[0047] In addition, since the present invention can be used without restriction as long as it is groundwater or tap water, it has the advantage of being able to cultivate shiitake mushrooms with increased vanadium and selenium content at a high yield in any location or region, domestically or internationally. Brief explanation of the drawing

[0049] FIG. 1 is a block diagram showing a preferred embodiment of the present invention. Figure 2 is an image of the packaging state of shiitake mushrooms cultivated according to the present invention. FIG. 3a is a report label for testing the vanadium content of shiitake mushrooms cultivated according to the present invention, and FIG. 3b is the back page of a test report on the vanadium content of shiitake mushrooms cultivated according to the present invention. Specific details for implementing the invention

[0050] The present invention will be described in more detail below through examples. These examples are intended solely to explain the invention more specifically, and it will be obvious to those skilled in the art that the scope of the invention is not limited by these examples according to the gist of the invention.

[0052] Cultivation method

[0053] 1. Preparation step of the culture solution

[0054] Four culture solutions of different concentrations were prepared as shown in Table 1 below using a vanadium aqueous solution (100 ml water + 100% pure vanadium) of 16–25 ppm in a water tank supplied with water.

[0055] Preparation Example Concentration and mixing ratio 1 Water + Aqueous solution of vanadium at a concentration of 16 ppm 2 Water + Aqueous solution of vanadium at a concentration of 25 ppm 3 Water + Aqueous solution of vanadium at a concentration of 22 ppm 4 Water + Aqueous solution of vanadium at a concentration of 19 ppm

[0057] Four culture solutions of different concentrations were prepared as shown in Table 2 below using an aqueous selenium solution (100 ml water + 100% pure selenium) of 16–25 ppm in a water tank supplied with water.

[0058] Preparation Example Concentration and mixing ratio 1 Water + Selenium aqueous solution with a concentration of 16 ppm 2 Water + Selenium solution with a concentration of 25 ppm 3 Water + Selenium solution with a concentration of 22 ppm 4 Water + Selenium solution at a concentration of 19 ppm

[0059] It includes mixing water with an ionized vanadium aqueous solution (10,000 ppm) to maintain the vanadium and selenium content at 20 ppm of the total content, and then cultivating by immersion, humidification, and spraying, and the concentrations and compositions of Tables 1 and 2 above include the vanadium and selenium aqueous solution being 10,000 ppm, and in order to maintain the content at 20 ppm, the water:vanadium (selenium) mixing ratio being 100 L of water:0.16 to 2.5 L of vanadium.

[0061] 2. Shiitake mushroom substrate inoculation stage

[0062] It includes the step of removing the outer shell and washing it at a temperature of 18–22 ℃ and a humidity of 85% or higher to perform granulation.

[0064] 3. Foot removal stage

[0065] It includes performing the pruning work for 3 to 5 days while maintaining conditions of daytime temperature of 18 to 28 ℃ and humidity of 85% or higher, and nighttime temperature of 5 to 15 ℃ and humidity of 85% or higher.

[0067] 4. Needle (Immersion) Step

[0068] In the first cycle, the weight of the culture medium is made to 3 kg using a conventional culture medium molding machine, and if the weight of the culture medium is maintained at 70 to 90 percent of the standard value, immersion is not required; however, if the weight of the culture medium falls below the standard value, the culture solution prepared in the culture medium preparation stage is immersed using an injector in the form of a conventional syringe needle to reach the standard value.

[0069] In the 2nd and 3rd cycles, since the weight of the substrate decreases due to dehydration during the growth process of the mushrooms, in the 2nd or 3rd cycle, the culture solution prepared in the culture solution preparation stage is immersed through an injector in the form of a conventional syringe needle up to 70 to 90% of the substrate weight, and the immersion step is performed at a temperature of 18 to 22 ℃ and a humidity of 85% or higher from the first cycle to the 2nd and 3rd cycles.

[0070] It includes replacing the above needle-needle step with a process of immersing in water containing a culture solution.

[0071] When cultivating general mushrooms, the needle-injection and immersion steps are omitted.

[0073] 5. Thinning stage

[0074] It is determined based on the germination status (number) of shiitake mushrooms, and involves thinning out all but about 8 mushrooms per substrate, and maintaining the temperature and humidity at the same level as the growth condition.

[0076] 6. Growth stage

[0077] For general mushrooms, temperature and humidity are set differently depending on the thermophilic, mesophilic, and psychrophilic varieties.

[0078] The high temperature of general mushrooms is maintained at 20–25°C, the medium temperature at 15–20°C, and the low temperature at 8–15°C, while the humidity is maintained at 80–85% to cultivate shiitake mushrooms.

[0079] In the case of the white flower mushroom (10), it is cultivated by repeatedly operating with variations in temperature and humidity to induce a whitening phenomenon.

[0080] In the case of Baekhwago (10), the temperature is raised from 6 ℃ to 28 ℃, and the humidity is lowered from 95% to 50% to perform dehumidification and maintain it for 2 hours.

[0081] After maintaining the above temperature and humidity for 2 hours, conversely, the temperature of 28 ℃ is lowered to 6 ℃, and the humidity is increased from 50% to 95% through humidification.

[0082] In the process of humidifying the humidity from 50% to 95%, a culture solution is supplied to the humidifier and humidification is performed for 2 hours through spraying, thereby allowing the vanadium aqueous solution and selenium aqueous solution contained in the culture solution supplied during the growth process of the white mushroom to be absorbed and the content increased.

[0083] The growth process of the above white mushroom (10) includes raising the temperature and lowering the humidity for 2 hours, and lowering the temperature and raising the humidity for 2 hours, repeating this process 4 to 10 times a day.

[0084] The above process of raising the temperature and lowering the humidity, and lowering the temperature and raising the humidity, includes allowing for differences in adjustment times depending on the condition of the mushrooms.

[0085] In the growth process of the above-mentioned white mushrooms (10), the temperature and humidity variations are repeatedly operated to induce whitening, thereby enabling the production of white mushrooms of excellent quality.

[0086] The above ordinary mushrooms and white mushrooms (10) can be harvested for 10 days starting from 10 days after the substrate is formed.

[0088] 7. Mixing ratio of vanadium aqueous solution and selenium aqueous solution

[0089] To produce shiitake mushrooms with increased vanadium and selenium content, shiitake mushrooms were cultivated using the culture solution of Preparation Example 1. Shiitake mushrooms grown for about 10 days were harvested, and a component analysis was requested from the Korea Ceramic Technology Institute (located in Bucheon-si, Gyeonggi-do) to confirm the vanadium and selenium content contained in the cultivated shiitake mushrooms.

[0091] 8. Recover (Obtain) Shiitake Mushrooms

[0092] Shiitake mushrooms were recovered through a step of recovering (obtaining) shiitake mushrooms grown for about 10 days (about 240 hours) (Fig. 2).

[0094] 9. Test Results

[0095] As a result of analyzing the test analysis items as shown in Table 3 below using the shiitake mushrooms recovered from item 8 as a sample, the vanadium content was analyzed to be 4.85 mg / kg, confirming that the content of the shiitake mushrooms had increased.

[0096] Sample name Test analysis items Test analysis results Test analysis method note shiitake mushrooms V (mg / kg)Cr (mg / kg)Zn (mg / kg) 4.8519.978.1 Wet analysis and instrumental analysis

[0097] Note) 1. The ICP-OES used in the above analysis is Perkin Elmer's Opima 5300 DV (077C6101602).

[0098] 2. The ICP-MS used in the above analysis is Perkin Elmer's ELAN DRC H (Q220202).

[0100] 10. Blood sugar lowering effect

[0101] Male mice weighing 200±10g were prepared, and diabetes was induced by injecting streptozotocin (SZT), known as a diabetes-inducing agent, into the abdominal cavity at a dose of 60mg / kg of mouse weight, and symptoms of diabetes were confirmed using a urine glucose test stick.

[0102] The above-mentioned diabetic mice were divided into 5 groups of 5 mice each, and each group was fed 2 ml of shiitake mushrooms and powdered shiitake mushrooms once daily for 5 weeks according to the above preparation examples and comparative examples 1 to 4. Blood glucose levels were then measured, and the average values ​​are shown in Table 4 below.

[0103] division Change in blood sugar (%) after 5 weeks Comparative Example 1 81.3 Comparative Example 2 82.1 Comparative Example 3 98.5 Comparative Example 4 98.4

[0104] Referring to Table 3 above, it can be seen that the blood sugar lowering effect is most pronounced when shiitake mushrooms are fed as they are, as in Comparative Example 1, or when shiitake mushrooms are dried and powdered, as in Comparative Example 2.

[0105] In addition, in the case of Comparative Example 3, ordinary shiitake mushrooms were used as they were, and in the case of Comparative Example 4, they were provided in powdered form, so vanadium and selenium were almost not contained, and thus it is presumed that there is almost no blood sugar lowering effect.

[0106] In this way, when shiitake mushrooms are cultivated as in the example and prepared into powder, shiitake mushroom powder with an increased absorption rate in the body upon consumption can be obtained.

[0107] Although the above description focused on examples of cultivation methods for shiitake mushrooms including white shiitake mushrooms, it includes methods that can be made to contain vanadium and selenium during the process of cultivating various types of mushrooms and vegetables.

[0109] Specific parts of the present invention have been described in detail above. It is evident to those skilled in the art that such specific descriptions are merely preferred embodiments and that the scope of the present invention is not limited thereto.

[0110] Accordingly, the substantial scope of the present invention will be defined by the appended claims and their equivalents. Industrial applicability

[0112] The present invention provides a very useful invention that enables increasing the vanadium content in high-temperature, medium-temperature, and low-temperature shiitake mushrooms (Baekhwago) by adjusting the weight of the substrate during the needle-injection process to reach a reference value and supplying it through humidification when humidity is improved during the process of controlling humidity in the growth process for harvesting.

Claims

Claim 1 A method for cultivating shiitake mushrooms containing vanadium, comprising: a) a step of preparing a culture solution by ionizing vanadium and selenium in water; b) a step of removing outer skin, washing, and granulation of the substrate; c) a step of germinating the substrate obtained from step b) for 3 to 5 days; d) a step of supplying the culture solution obtained from step a) to perform needle insertion so that the weight of the substrate is maintained at 70 to 90% of a reference value based on 3 kg; e) a step of thinning out the mushrooms, leaving 8 mushrooms per substrate, according to the germination state of the shiitake mushrooms; f) a step of growing general shiitake mushrooms and white shiitake mushrooms; wherein the general shiitake mushrooms are divided into high-temperature, medium-temperature, and low-temperature types to control the temperature, and the white shiitake mushrooms are subjected to a process of raising the temperature and lowering the humidity, and then lowering the raised temperature and raising the humidity repeatedly, and a step of spraying the culture solution during humidification while raising the humidity; and g) a step of harvesting the grown shiitake mushrooms. Claim 2 A method for cultivating vanadium-containing shiitake mushrooms according to claim 1, wherein step a) comprises preparing a culture solution using 16 to 25 ppm of a vanadium aqueous solution (100 ml of water + 100% pure vanadium) in a water tank supplied with water. Claim 3 A method for cultivating vanadium-containing shiitake mushrooms according to claim 1, wherein step f) controls the temperature by dividing the high-temperature nature of general shiitake mushrooms into 20–25 ℃, the medium-temperature nature into 15–20 ℃, and the low-temperature nature into 8–15 ℃, and sprays a culture solution to grow them when the humidity is controlled to 80–85%. Claim 4 A method for cultivating vanadium-containing shiitake mushrooms according to claim 1, wherein step f) involves repeating the process of raising the temperature of the white shiitake mushrooms from 6 ℃ to 28 ℃ and lowering the humidity from 95% to 50%, lowering the raised temperature from 28 ℃ to 6 ℃ and raising the humidity from 50% to 95%, and repeating the process of controlling the humidity by spraying a culture solution during the process of raising the humidity every 2 hours.

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