Composition for Preventing Hair Loss and Promoting Hair Growth Containing Plant Cell Culture Extract

Abstract

The present invention relates to a composition for preventing hair loss and promoting hair growth comprising a plant cell culture extract, characterized by comprising: a plant cell culture extract comprising ginseng callus culture extract, green tea callus culture extract, and Chrysanthemum indicum callus culture extract in a weight ratio of 2 to 4:1 to 2:1; auxiliary components such as RH-oligopeptide-2 and RH-polypeptide-1; a moisturizer comprising glycerin and sodium hyaluronate; a sedative comprising Centella asiatica extract, panthenol, madecassoside, and allantoin; and a scalp microbiome regulating component comprising Lactobacillus fermentation lysate and Bifida fermentation lysate. The composition according to the present invention provides excellent effects for preventing hair loss and promoting hair growth by achieving effects such as promoting hair papilla cell proliferation, extending the hair follicle growth phase, inhibiting DHT production, and normalizing the scalp microbiome.

Description

Technology Field

[0001] The present invention relates to a composition for preventing hair loss and promoting hair growth that includes a plant cell culture extract as an active ingredient and exhibits excellent effects in preventing hair loss, soothing the scalp, moisturizing, providing nutrition, and promoting hair growth. Background Technology

[0002] The problem of hair loss is becoming increasingly serious due to modern lifestyles, stress, environmental pollution, and hormonal imbalances. Beyond being a mere cosmetic issue, hair loss has become a major cause of psychological stress and a decline in quality of life, and the global population experiencing hair loss is on a continuous upward trend.

[0003] Major causes of hair loss include androgenetic alopecia, alopecia areata, and telogen effluvium. Among these, androgenetic alopecia is the most common form and occurs through a mechanism in which testosterone is converted into dihydrotestosterone (DHT) by genetic factors and 5-alpha-reductase, causing hair follicles to atrophy. Ingredients such as finasteride, dutasteride, and minoxidil have been used to inhibit this, but there have been limitations to long-term use due to reported side effects such as sexual dysfunction, scalp irritation, and contact dermatitis.

[0004] In particular, existing hair loss care products have often relied primarily on chemically synthesized ingredients or used simple plant extracts, resulting in minimal or unstable effects. Therefore, there has been an urgent need for the development of safe and effective natural-derived compositions that can promote the activity of hair follicle cells and induce healthy hair growth without irritating the scalp.

[0005] Plant cell culture extracts are components extracted from cells obtained by proliferating plant callus or meristematic tissue cells in an artificial culture environment, and contain high concentrations of the plant's natural active physiological substances. In addition, they are not affected by external environmental factors such as season, climate, or soil pollution, allowing for the maintenance of stable and uniform quality, and have the advantage of enabling the sustainable utilization of active ingredients from rare or endangered plants.

[0006] However, conventional scalp care compositions utilizing plant cell culture extracts have been limited to using a single plant cell culture extract or simply mixing plant cell culture extracts with general plant extracts; consequently, no composition has been known that simultaneously satisfies the core mechanisms of hair growth, namely the promotion of dermal papilla cell proliferation and the extension of the anagen phase. Furthermore, no composition has been reported that simultaneously achieves scalp environment improvement and hair growth promotion effects through the regulation of the scalp microbiome. The problem to be solved

[0007] The present invention aims to provide a composition for preventing hair loss and promoting hair growth that includes a specific plant cell culture extract as an active ingredient to solve the problems of the aforementioned prior art, while minimizing scalp irritation and providing effects such as activating hair follicle cells, preventing hair loss, promoting hair growth, soothing the scalp, and moisturizing.

[0008] More specifically, the present invention aims to provide a composition that simultaneously achieves the effects of promoting the proliferation of hair papilla cells, extending the hair follicle growth phase, inhibiting DHT production, and normalizing the scalp microbiome by combining a plurality of plant cell culture extracts, such as ginseng callus culture extract, carrot callus culture extract, green tea callus culture extract, chrysanthemum callus culture extract, mung bean meristematic tissue cell culture extract, and Bambusa vulgaris callus culture extract, in specific proportions and combining them with a scalp microbiome regulating component. means of solving the problem

[0009] According to an embodiment of the present invention, a composition for preventing hair loss and promoting hair growth comprises: two or more plant cell culture extracts selected from the group consisting of ginseng callus culture extract, carrot callus culture extract, green tea callus culture extract, chrysanthemum callus culture extract, mung bean meristematic tissue cell culture extract, and Bambusa vulgaris callus culture extract; one or more auxiliary components selected from the group consisting of caprylyl glycol, tropolone, biotin, RH-oligopeptide-2, RH-polypeptide-1, and ethylhexylglycerin; one or more scalp microbiome regulating components selected from the group consisting of Lactobacillus fermentation lysate and Bifida fermentation lysate; one or more moisturizers selected from the group consisting of glycerin and sodium hyaluronate; and one or more soothing agents selected from the group consisting of Centella asiatica extract, panthenol, madecassoside, and allantoin.

[0010] In addition, in the present invention, it is preferable that the plant cell culture extract comprises ginseng callus culture extract, green tea callus culture extract, and chrysanthemum callus culture extract as essential components. The weight ratio of the ginseng callus culture extract, green tea callus culture extract, and chrysanthemum callus culture extract is 2 to 4 : 1 to 2 : 1.

[0011] In addition, the composition for preventing hair loss and promoting hair growth preferably comprises, based on 100 parts by weight of purified water, 0.1 to 2 parts by weight of the ginseng callus culture extract, 0.05 to 1 part by weight of the green tea callus culture extract, 0.05 to 1 part by weight of the Chrysanthemum indicum callus culture extract, 0.1 to 2 parts by weight of the scalp microbiome regulating component, 0.01 to 0.5 parts by weight of the RH-oligopeptide-2, 0.01 to 0.5 parts by weight of the RH-polypeptide-1, 1 to 10 parts by weight of the glycerin, 0.01 to 0.5 parts by weight of the sodium hyaluronate, 0.1 to 2 parts by weight of the Centella asiatica extract, 0.1 to 2 parts by weight of the panthenol, and 0.01 to 0.5 parts by weight of the allantoin.

[0012] Additionally, based on 100 parts by weight of purified water, a surfactant comprising 7.0 parts by weight of Lauramidopropyl Betaine, 3.5 parts by weight of Disodium Laureth Sulfosuccinate, 2.5 parts by weight of Sodium Cocoyl Isethionate, 2.0 parts by weight of Sodium Methyl Cocoyl Taurate, 1.0 parts by weight of Sodium Cocoyl Glutamate, and 0.09 parts by weight of Cocamide Methyl MEA; and a preservative comprising 0.03 parts by weight of Disodium EDTA and 0.01 parts by weight of Hydroxyacetophenone; It is characterized by being a shampoo formulation further comprising an acidity regulator comprising 0.10 parts by weight of citric acid and 0.10 parts by weight of sodium citrate. Effects of the invention

[0013] The present invention provides plant cell culture extracts, such as ginseng callus culture extract, carrot callus culture extract, green tea callus culture extract, chrysanthemum callus culture extract, mung bean meristematic tissue cell culture extract, and Bambusa vulgaris callus culture extract, which promote the proliferation of hair papilla cells and extend the growth phase of hair follicles, thereby preventing hair loss and promoting hair growth.

[0014] In particular, the combination of ginseng callus culture extract, green tea callus culture extract, and chrysanthemum callus culture extract provides a significantly improved effect in terms of hair papilla cell proliferation rate and scalp anti-inflammatory effects compared to using each individually.

[0015] The active ingredients of plant cell culture extracts enhance the vitality of scalp cells, and among them, Chrysanthemum indicum callus culture extract can inhibit scalp aging through its antioxidant action. Furthermore, it exerts a synergistic effect with soothing ingredients such as tropolone, Centella asiatica extract, and panthenol to effectively soothe a scalp sensitized by external stimuli and provide a scalp soothing effect that alleviates itching. Specific details for implementing the invention

[0016] The present invention is capable of various modifications and may take various forms, and embodiments (aspects or examples) are to be described in detail in the text. However, this is not intended to limit the present invention to the specific disclosed forms, and it should be understood that it includes all modifications, equivalents, and substitutions that fall within the spirit and scope of the invention.

[0017] The terms used in this specification are used merely to describe specific embodiments (aspects or examples) and are not intended to limit the invention. Singular expressions include plural expressions unless the context clearly indicates otherwise. In this application, terms such as “comprising” or “consisting of” are intended to indicate the existence of the features, numbers, steps, actions, components, parts, or combinations thereof described in the specification, and should be understood as not precluding the existence or addition of one or more other features, numbers, steps, actions, components, parts, or combinations thereof.

[0018] Unless otherwise defined, all terms used herein, including technical or scientific terms, have the same meaning as generally understood by those skilled in the art to which the present invention pertains. Terms such as those defined in commonly used dictionaries should be interpreted as having a meaning consistent with their meaning in the context of the relevant technology, and should not be interpreted in an ideal or overly formal sense unless explicitly defined in this application.

[0019] The terms "~1~," "~2~," etc., described in this specification are used merely to distinguish different components and are not bound by the order of manufacture; furthermore, the names may not match those in the detailed description of the invention and the claims.

[0020] The present invention relates to a composition for preventing hair loss and promoting hair growth comprising, as an active ingredient, two or more plant cell culture extracts selected from the group consisting of ginseng callus culture extract, carrot callus culture extract, green tea callus culture extract, chrysanthemum callus culture extract, mung bean meristematic tissue cell culture extract, and Bambusa vulgaris callus culture extract.

[0021] The term 'plant cell culture extract' used in the present invention refers to a component extracted from cells obtained by proliferating plant callus or other tissues in an artificial culture environment. Such an extract contains a high concentration of unique physiologically active substances derived from specific plants and has the advantage of maintaining stable quality and a uniform content of active ingredients regardless of external environmental contamination or seasonal changes.

[0022] In addition to the plant cell culture extract, the composition of the present invention may further include one or more auxiliary ingredients that provide scalp soothing, moisturizing, and nutrient supply effects.

[0023] The types and contents of plant cell culture extracts included in the composition of the present invention are as follows. The content of each component is expressed in parts by weight per 100 parts by weight of purified water.

[0024] The ginseng callus culture extract may contain 0.01 to 5 parts by weight, preferably 0.1 to 2 parts by weight. It contains a high concentration of ginsenoside components characteristic of ginseng, improves blood circulation in the scalp, promotes the proliferation of hair papilla cells, and induces hair growth.

[0025] The carrot callus culture extract may be included in an amount of 0.01 to 3 parts by weight, preferably 0.05 to 1 part by weight. The carrot callus culture extract is rich in beta-carotene and vitamins, which promote the regeneration of scalp cells and give shine to the hair.

[0026] The green tea callus culture extract may contain 0.01 to 3 parts by weight, preferably 0.05 to 1 part by weight. It contains a high concentration of polyphenol components such as catechin from green tea, thereby preventing hair loss through powerful antioxidant action and the effect of inhibiting DHT production by inhibiting 5-alpha-reductase.

[0027] The Chrysanthemum indicum callus culture extract may contain 0.01 to 3 parts by weight, preferably 0.05 to 1 part by weight. Chrysanthemum indicum contains flavonoid components that suppress inflammation around hair follicles and improve the scalp environment through antioxidant and anti-inflammatory actions.

[0028] The mung bean meristematic tissue cell culture extract may be included in an amount of 0.01 to 3 parts by weight, preferably 0.05 to 1 part by weight. The mung bean meristematic tissue cells promote the activation of hair follicle cells and the secretion of hair growth factors, thereby providing a hair growth effect.

[0029] The Bambusa vulgaris callus culture extract may be included in an amount of 0.01 to 3 parts by weight, preferably 0.05 to 1 part by weight. Bambusa vulgaris leaves are rich in silica, which increases hair strength and strengthens hair follicles.

[0030] Among the above plant cell culture extracts, the ginseng callus culture extract, green tea callus culture extract, and chrysanthemum callus culture extract are essential components of the present invention, and it is preferable that the weight ratio of these three types is in the range of ginseng callus culture extract : green tea callus culture extract : chrysanthemum callus culture extract = 2~4 :1~2 :1.

[0031] In addition to the plant cell culture extract, the composition of the present invention may include one or more auxiliary ingredients that provide effects such as preventing hair loss, promoting hair growth, soothing the scalp, moisturizing, and conditioning.

[0032] Caprylyl glycol is an ingredient that keeps the scalp clean through moisturizing and antibacterial action, and it is preferable to include 0.1 to 1 part by weight per 100 parts by weight of purified water.

[0033] Tropolone is an ingredient that provides antibacterial and scalp soothing effects, and it is preferable to include 0.01 to 0.5 parts by weight per 100 parts by weight of purified water.

[0034] Biotin is vitamin B7, which is essential for hair growth, and is a component that promotes keratin synthesis. It is preferable to include 0.01 to 0.5 parts by weight of biotin per 100 parts by weight of purified water.

[0035] rh-oligopeptide-2 is an epidermal growth factor (EGF)-like peptide that promotes the proliferation of hair follicle cells. It is preferable to include 0.01 to 0.5 parts by weight of this per 100 parts by weight of purified water.

[0036] rh-polypeptide-1 is a fibroblast growth factor (FGF)-like peptide and is a component that contributes to the activation of dermal papilla cells; it is preferable to include 0.01 to 0.5 parts by weight per 100 parts by weight of purified water.

[0037] Ethylhexylglycerin is an ingredient that provides moisturizing and skin conditioning effects, and it is preferable to include 0.1 to 1 part by weight per 100 parts by weight of purified water.

[0038] The composition of the present invention preferably comprises, as a scalp microbiome modulator, one or more selected from the group consisting of Lactobacillus ferment lysate and Bifida ferment lysate in an amount of 0.1 to 2 parts by weight per 100 parts by weight of purified water.

[0039] Scalp microbiome regulating ingredients strengthen the scalp barrier function by activating beneficial bacteria on the scalp and suppressing the overgrowth of harmful bacteria associated with hair loss. In addition, by inhibiting the secretion of inflammatory cytokines (IL-1β, TNF-α) around hair follicles, they prevent hair follicle atrophy and exhibit the effect of extending the growth phase of hair follicles.

[0040] In addition, the moisturizer may be selected from the group consisting of glycerin and sodium hyaluronate, and it is preferable to include 1 to 10 parts by weight of glycerin and 0.01 to 0.5 parts by weight of sodium hyaluronate per 100 parts by weight of purified water.

[0041] Glycerin forms a moisture film on the surface of the skin to prevent moisture loss from the scalp, and sodium hyaluronate absorbs and retains moisture equivalent to hundreds of times its own weight, thereby supplying moisture deep into the scalp and contributing to maintaining a healthy scalp environment.

[0042] In addition, the sedative may be selected from one or more of the group consisting of Centella asiatica extract, panthenol, madecassoside, allantoin, etc., and it is preferable to include 0.1 to 2 parts by weight of Centella asiatica extract, 0.1 to 2 parts by weight of panthenol, 0.01 to 0.5 parts by weight of madecassoside, and 0.01 to 0.5 parts by weight of allantoin per 100 parts by weight of purified water.

[0043] Centella asiatica extract and madecassoside contain components such as asiaticoside and madecassic acid to provide scalp soothing and skin barrier strengthening effects, while panthenol promotes scalp cell regeneration, and allantoin can provide scalp itching relief and cell regeneration effects.

[0044] The composition of the present invention can be manufactured into various scalp care product formulations, such as shampoo, conditioner, treatment, hair essence, scalp tonic, scalp pack, and cleansing cream.

[0045] When the composition of the present invention is prepared as a shampoo formulation, it may further include a surfactant in an amount of 14 to 18 parts by weight per 100 parts by weight of purified water. Specifically, it is preferable to include 7.0 parts by weight of Lauramidopropyl Betaine, 3.5 parts by weight of Disodium Laureth Sulfosuccinate, 2.5 parts by weight of Sodium Cocoyl Isethionate, 2.0 parts by weight of Sodium Methyl Cocoyl Taurate, 1.0 parts by weight of Sodium Cocoyl Glutamate, and 0.09 parts by weight of Cocamide Methyl MEA.

[0046] All surfactants are composed of naturally derived or hypoallergenic ingredients, allowing them to provide effective cleansing power without irritating even sensitive scalps.

[0047] Lauramidopropyl Betaine (7.00 parts by weight) is a very mild amphoteric surfactant that provides cleansing power, foaming power, and conditioning effects, and also has an antistatic effect. When mixed with other surfactants, it alleviates the irritation of anionic surfactants. If the amount of Lauramidopropyl Betaine is less than 7 parts by weight, the cleansing power and foaming power may not be sufficient, and if it exceeds 7 parts by weight, it may dry out the skin and hair.

[0048] Disodium Laureth Sulfosuccinate (3.50 parts by weight) is a mild cleansing ingredient that causes minimal skin irritation and has good lathering power. It is less irritating than general sulfates and provides an efficient cleansing effect. If the amount of disodium laureth sulfosuccinate is less than 3.5 parts by weight, sufficient lather is not generated, resulting in reduced cleansing power; if it exceeds 3.5 parts by weight, it can be irritating to the scalp and cause the scalp and hair to become dry overall.

[0049] Sodium Cocoyl Isethionate (2.50 parts by weight) is a gentle anionic surfactant derived from coconut oil. It is mild to the skin and hair and has a moisturizing effect, helping to remove sebum and provide moist cleansing. It forms a rich, creamy lather.

[0050] Sodium Methyl Cocoyl Taurate (2.00 parts by weight) is also a natural surfactant derived from coconut, providing effective cleansing power while being gentle on the skin. It lathers well and causes minimal skin irritation, making it suitable for sensitive skin, while relieving dryness and leaving a soft texture after use.

[0051] Sodium Cocoyl Glutamate (1.00 parts by weight) is a naturally derived surfactant based on coconut oil and glutamic acid. It is gentle on the skin and scalp and is mainly used in cleansers and shampoos. It provides gentle cleansing power, creates rich lather, and stabilizes the foam.

[0052] Cocamide Methyl MEA (0.09 parts by weight) is a compound of fatty acids and ethanolamine extracted from coconut oil, and is mainly used to create foam and control viscosity in cleaning products such as shampoos and soaps.

[0053] Additionally, collagen tripeptide may be included in an amount of 0.5 parts by weight per 100 parts by weight of purified water, and may provide the effects of restoring elasticity to the dermal layer of the scalp and anti-inflammatory action.

[0054] In addition, it may further include a preservative comprising 0.03 parts by weight of disodium EDTA and 0.01 parts by weight of hydroxyacetophenone per 100 parts by weight of purified water, and an acidity regulator comprising 0.1 parts by weight of citric acid and 0.1 parts by weight of sodium citrate per 100 parts by weight of purified water.

[0055] The acidity regulator plays a role in maintaining the pH of the final composition at 5.0 to 7.0 to minimize scalp irritation and ensure formulation stability.

[0057] [Manufacturing Method]

[0058] An example of a method for preparing a composition according to the present invention is as follows. A shampoo example will be used as an example.

[0059] (1) Add a moisturizer, a soothing agent, and other water-soluble ingredients to purified water and mix uniformly.

[0060] (2) Mix the surfactant in a separate container.

[0061] (3) Slowly add the surfactant mixture of (2) to the mixture of (1) while stirring.

[0062] (4) Plant cell culture extract, scalp microbiome regulating ingredients, auxiliary ingredients, etc. are sequentially added to the above mixture and mixed uniformly.

[0063] (5) If necessary, use an acidity regulator to adjust the final pH to 5.0 to 7.0.

[0064] [Example 1: Shampoo composition for preventing hair loss and promoting hair growth]

[0065] A shampoo for preventing hair loss and promoting hair growth was prepared with the composition shown in Table 1 below. All contents are based on 100 parts by weight of purified water.

[0067] Example 1 Composition (based on 100 parts by weight of purified water) division Ingredient name Content plant cell culture extract Ginseng callus culture extract 0.3 Carrot callus culture extract 0.2 Green tea callus culture extract 0.15 Chrysanthemum indicum callus culture extract 0.1 Mung bean meristematic tissue cell culture extract 0.1 Bambusa vulgaris callus culture extract 0.05 auxiliary ingredients Caprylyl glycol 0.5 Tropolon 0.05 biotin 0.1 RH-Oligopeptide-2 0.1 RH-Polypeptide-1 0.1 Ethylhexylglycerin 0.3 moisturizer glycerin 3.0 Sodium hyaluronate 0.1 sedative Centella asiatica extract 0.5 Panthenol 0.5 Madecassoside 0.05 allantoin 0.1 Scalp microbiome regulating ingredients Lactobacillus fermented lysate 0.5 Bifida fermented lysate 0.3 surfactants Lauramidopropyl Betaine 7.0 disodium laureth sulfosuccinate 3.5 Sodium cocoyl isethionate 2.5 Sodium methyl cocoyl taurate 2.0 Sodium cocoyl glutamate 1.0 Cocamide-methyl MEA 0.09 preservatives Disodium EDTA 0.03 Hydroxyacetophenone 0.01 pH regulator Citric acid 0.1 Sodium citrate 0.1

[0069] In the above Example 1, the ratio of ginseng callus culture extract (0.3 parts by weight) : green tea callus culture extract (0.15 parts by weight) : chrysanthemum callus culture extract (0.1 parts by weight) is approximately 3 : 1.5 : 1, which falls within the preferred range of 2 to 4 : 1 to 2 : 1.

[0071] [Comparative Example]

[0072] To verify the effects of the present invention, comparative examples were set up as shown in Table 2 below.

[0074] division Key Features Comparative Example 1 Not containing plant cell culture extract (replaced with purified water) Comparative Example 2 Contains only general plant extracts (ginseng extract 0.3, green tea extract 0.3 parts by weight) Comparative Example 3 Contains plant cell culture extract, does not contain scalp microbiome regulating ingredients Comparative Example 4 The combination ratio of ginseng, green tea, and chrysanthemum is 6:2:1

[0076] [Experimental Example]

[0077] Experimental Example 1 : Evaluation of Dermal Papilla Cell Proliferation Effect

[0078] To evaluate the effect of the composition according to the present invention on the proliferation of dermal papilla cells, a cell proliferation experiment using human dermal papilla cells was performed. Human dermal papilla cells were seeded into a 96-well plate at a density of 1×10^4 cells / well and cultured for 24 hours, after which each test substance was treated at a concentration of 0.1%.

[0079] Cell viability was measured using the MTT assay after an additional 48 hours of culture.

[0080] division Cell proliferation rate (%) Increase rate (%) compared to the control group Comparative Example 1 100.0 - Comparative Example 2 118.5 +18.5 Comparative Example 3 141.3 +41.3 Comparative Example 4 143.2 +43.2 Example 1 172.6 +72.6

[0082] According to the results in Table 3 above, Example 1 of the present invention showed a 72.6% effect in promoting hair papilla cell proliferation compared to Comparative Example 1, which lacked plant cell culture extract. In particular, since Example 1 showed a significantly superior effect even compared to Comparative Example 3, which lacked scalp microbiome regulating components, it was confirmed that the addition of scalp microbiome regulating components plays a key role in promoting hair growth. Furthermore, since Example 1 showed a significantly superior effect even compared to Comparative Example 4, in which the ratio of ginseng:green tea:chrysanthemum callus culture extracts fell outside the optimal range, the optimal ratio of the three components (2~4 : 1~2 : 1) could be confirmed.

[0084] Experimental Example 2 : Propagation rate of dermal papilla cells according to the ratio of 3 types of plant cell culture extracts

[0085] division Ginseng:Green Tea:Chrysanthemum Ratio Cell proliferation rate (%) A 1:1:1 137.4 B 2:1:1 154.8 C 3:1.5:1 172.6 D 4:2:1 171.3 E 4:1:1 157.9 F 6:2:1 143.2

[0086] According to the results in Table 4, the proliferation rate of dermal papilla cells was high in the ratio of ginseng callus culture extract : green tea callus culture extract : chrysanthemum callus culture extract in the range of 2~4 : 1~2 : 1 (B, C, D), and it was confirmed that the proliferation rate significantly decreased when the ratio fell outside this range (A, E, F).

[0087] Experimental Example 3 : Scalp and hair condition evaluation (sensory evaluation)

[0088] Evaluations were conducted on scalp troubles, hair damage, the resilience of damaged scalp and hair, and the condition of the hair texture. The evaluation was performed using a 5-point sensory evaluation (5: very good, 4: good, 3: average, 2: poor, 1: very poor). The evaluation method involved 20 adult men and women with damaged hair and scalp troubles using each composition daily for 14 days, and the average of the scores was used as the evaluation value.

[0090] Evaluation results (rating) for damaged scalp and hair Evaluation criteria Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Relieves scalp troubles 4.8 2.5 3.2 3.9 Improve hair damage 4.7 2.4 3.0 3.7 Damaged scalp / hair recovery 4.9 2.3 3.1 3.8 Improve hair texture 4.8 2.6 3.3 3.9 Feeling relief from hair loss 4.7 2.2 3.0 3.6

[0091] According to the results in Table 5, it was confirmed that Example 1 according to the present invention received a significantly superior evaluation compared to the comparative examples in all evaluation categories. Comparative Example 1, which did not contain plant cell culture extract, showed low scores in all evaluation categories, proving that the plant cell culture extract, which is the active ingredient of the present invention, plays an essential role in improving the scalp and alleviating hair loss. In particular, even when compared to Comparative Example 3, which lacks a scalp microbiome regulating component, Example 1 obtained significantly higher scores in all categories, confirming that the addition of a scalp microbiome regulating component significantly enhances the effects of preventing hair loss and promoting hair growth.

[0092] This suggests that the composition of the present invention has superior efficacy compared to existing products in preventing hair loss, soothing the scalp, moisturizing, providing nutrition, and strengthening hair, and in particular provides excellent effects in restoring damaged scalp and hair.

Claims

Claim 1 A composition for preventing hair loss and promoting hair growth, comprising: two or more plant cell culture extracts selected from the group consisting of ginseng callus culture extract, carrot callus culture extract, green tea callus culture extract, chrysanthemum callus culture extract, mung bean meristematic tissue cell culture extract, and Bambusa vulgaris callus culture extract; an auxiliary component comprising RH-oligopeptide-2 and RH-polypeptide-1; a scalp microbiome regulating component comprising Lactobacillus ferment lysate and Bifida ferment lysate; and one or more moisturizers selected from the group consisting of glycerin and sodium hyaluronate. and comprises one or more sedatives selected from the group consisting of Centella asiatica extract, panthenol, madecassoside, and allantoin, wherein the plant cell culture extract comprises ginseng callus culture extract, green tea callus culture extract, and Chrysanthemum indicum callus culture extract as essential components, and wherein the weight ratio of the ginseng callus culture extract, green tea callus culture extract, and Chrysanthemum indicum callus culture extract is 2~4 : 1~2 : 1, and the composition for preventing hair loss and promoting hair growth comprises, based on 100 parts by weight of purified water, 0.1 to 2 parts by weight of the ginseng callus culture extract, 0.05 to 1 part by weight of the green tea callus culture extract, 0.05 to 1 part by weight of the Chrysanthemum indicum callus culture extract, 0.1 to 2 parts by weight of the scalp microbiome regulating component, and 0.01 to RH-oligopeptide-2 A composition for preventing hair loss and promoting hair growth, characterized by comprising 0.5 parts by weight of the above-mentioned RH-polypeptide-1, 0.01 to 0.5 parts by weight of the above-mentioned glycerin, 0.01 to 0.5 parts by weight of the above-mentioned sodium hyaluronate, 0.1 to 2 parts by weight of the above-mentioned Centella asiatica extract, 0.1 to 2 parts by weight of the above-mentioned panthenol, and 0.01 to 0.5 parts by weight of the above-mentioned allantoin. Claim 2 delete Claim 3 delete Claim 4 In claim 1, a surfactant comprising, based on 100 parts by weight of purified water, 7.0 parts by weight of Lauramidopropyl Betaine, 3.5 parts by weight of Disodium Laureth Sulfosuccinate, 2.5 parts by weight of Sodium Cocoyl Isethionate, 2.0 parts by weight of Sodium Methyl Cocoyl Taurate, 1.0 parts by weight of Sodium Cocoyl Glutamate, and 0.09 parts by weight of Cocamide Methyl MEA; and a preservative comprising 0.03 parts by weight of Disodium EDTA and 0.01 parts by weight of Hydroxyacetophenone; A composition for preventing hair loss and promoting hair growth, characterized by being a shampoo formulation further comprising an acidity regulator comprising 0.10 parts by weight of citric acid and 0.10 parts by weight of sodium citrate.