Cosmetic composition for repairing skin damage caused by ultraviolet rays and method for preparing the same

Abstract

The present invention relates to a cosmetic composition for repairing skin damage caused by ultraviolet rays and a method for preparing the same. More specifically, the invention relates to a cosmetic composition and a method for preparing the said cosmetic composition that can effectively alleviate skin inflammation by inhibiting DNA damage and photodamage to skin cells along with excellent antioxidant and anti-inflammatory activities, strengthen skin barrier function by reducing transepidermal water loss (TEWL), and improve moisturizing power and elasticity. To this end, the said cosmetic composition comprises a naturally derived polysaccharide; and Icelandic moss extract as active ingredients.

Description

Technology Field

[0001] The present invention relates to a cosmetic composition for repairing skin damage caused by ultraviolet rays and a method for preparing the same. More specifically, the invention relates to a cosmetic composition and a method for preparing the said cosmetic composition that can effectively alleviate skin inflammation by inhibiting DNA damage and photodamage to skin cells along with excellent antioxidant and anti-inflammatory activities, strengthen skin barrier function by reducing transepidermal water loss (TEWL), and improve moisturizing power and elasticity. Background Technology

[0002] As average life expectancy increases due to advancements in medicine, the desire to maintain a youthful and healthy state for as long as possible is growing among people of all ages and genders, and interest in maintaining and improving skin condition—which is the most easily visible aspect of appearance—is particularly increasing. Accordingly, research is actively being conducted on functional materials that can identify the main causes of skin aging and prevent or improve it.

[0003] Skin aging is generally classified into intrinsic aging and extrinsic aging, and among these, the most major cause of extrinsic aging is photoaging caused by ultraviolet (UV) rays.

[0004] Ultraviolet rays are classified into UVA (320 to 400 nm), UVB (290 to 320 nm), and UVC (200 to 290 nm) depending on their wavelength. Of these, UVC is mostly blocked by the ozone layer, but UVA and UVB reach the skin directly and cause various forms of skin damage. In particular, UVA penetrates deep into the dermis layer of the skin to induce chronic photoaging, while UVB acts on the epidermis and upper dermis to cause sunburn and acute inflammatory reactions.

[0005] Such exposure to ultraviolet rays rapidly increases the production of reactive oxygen species (ROS) within the skin, and the excessively generated ROS induce lipid peroxidation of cell membranes, protein denaturation, and DNA damage, thereby impairing the function of skin cells. In addition, ROS disrupt intracellular signaling pathways, increasing the expression of matrix metalloproteinases (MMPs), which are collagen-degrading enzymes, particularly MMP-1, and consequently promoting the degradation of collagen and elastin, which are major structural proteins of the dermis.

[0006] Furthermore, ultraviolet rays promote the breakdown of hyaluronic acid, which plays a key role in maintaining skin hydration and elasticity, and inhibit its biosynthesis, thereby reducing the skin's ability to retain moisture. Consequently, this accelerates skin dryness, loss of elasticity, and wrinkle formation. These changes go beyond simple signs of aging and can lead to complex skin problems, such as damage to the skin barrier, increased inflammatory responses, and pigmentation.

[0007] Meanwhile, intrinsic aging also causes a decline in skin elasticity and the formation of wrinkles as the skin's physiological functions deteriorate over time and the ability to synthesize extracellular matrix components such as collagen, elastin, and hyaluronic acid decreases; however, it is recently known that external environmental factors, such as ultraviolet rays, act as major factors that further accelerate this intrinsic aging.

[0008] Therefore, there is a need to develop cosmetic compositions that can effectively inhibit the excessive generation of reactive oxygen species induced by ultraviolet rays and the resulting collagen degradation, hyaluronic acid reduction, and cell damage, or restore already damaged skin tissue.

[0009] Meanwhile, the aforementioned background technology is technical information that the inventor possessed for the derivation of the present invention or acquired during the process of deriving the present invention, and it cannot necessarily be considered publicly known technology disclosed to the general public prior to the filing of the present invention. Prior art literature

[0010] Republic of Korea Registered Patent Publication No. 10-2085492 The problem to be solved

[0011] The present invention aims to solve the above-mentioned problems by providing a cosmetic composition and a method for manufacturing the same, which can effectively inhibit the excessive generation of reactive oxygen species induced by ultraviolet rays and the resulting collagen degradation, reduction of hyaluronic acid, and cell damage, or restore already damaged skin tissue.

[0012] The above and other objects and advantages of the present invention will become apparent from the following description describing preferred embodiments. means of solving the problem

[0013] The above objective can be achieved by a cosmetic composition comprising a naturally derived polysaccharide and an Icelandic moss extract as active ingredients.

[0014] Specifically, the natural-derived polysaccharide may be characterized by comprising one or more from the group consisting of plant-derived polysaccharides, fungi-derived polysaccharides, animal-derived polysaccharides, and combinations thereof.

[0015] Specifically, the active ingredient may be characterized by being included in an amount of 0.01 to 10 weight percent relative to the total weight of the cosmetic composition.

[0016] Specifically, the cosmetic composition may be characterized by additionally including one or more additives selected from the group consisting of skin conditioning agents, thickeners, emulsifiers, neutralizing agents, antioxidants, chelating agents, fragrances, and combinations thereof.

[0017] Specifically, the cosmetic composition may be characterized as being a formulation selected from the group consisting of toner, lotion, essence, gel, mask pack, powder, balm, cleanser, and combinations thereof. Effects of the invention

[0018] According to the present invention, a cosmetic composition can be provided that can effectively inhibit DNA damage and photodamage to skin cells induced by ultraviolet rays or promote the recovery of already damaged skin tissue. In particular, it can contribute to maintaining the structural stability of the skin by reducing cell damage caused by reactive oxygen species increased by ultraviolet exposure and by regulating signaling pathways that induce collagen degradation and skin aging.

[0019] The cosmetic composition according to the present invention exhibits excellent antioxidant and anti-inflammatory effects, thereby alleviating skin inflammatory reactions caused by ultraviolet rays, reducing skin irritation, and helping to maintain skin cell homeostasis. Furthermore, by improving lipid components that strengthen skin barrier function, it can reduce transepidermal water loss (TEWL) and protect the skin from harmful external environments, thereby contributing to the maintenance of skin hydration and elasticity.

[0020] In addition, the cosmetic composition according to the present invention can promote the normal differentiation of keratinocytes and increase the expression of filaggrin and tight junction-related proteins to improve the structural integrity of the skin barrier, thereby simultaneously improving the skin's moisture retention ability and resistance to external stimuli.

[0021] Furthermore, the cosmetic composition according to the present invention has the advantage of being safe for application even to sensitive skin due to low skin irritation, and exhibits excellent physical and chemical stability when applied to various formulations, thereby minimizing quality changes even during long-term storage and use.

[0022] However, the effects of the present invention are not limited to those mentioned above, and other unmentioned effects will be clearly understood by those skilled in the art from the description below. Specific details for implementing the invention

[0023] The present invention will be described in detail below with reference to the embodiments and drawings. These embodiments are presented merely as examples to explain the invention more specifically, and it will be obvious to those skilled in the art that the scope of the invention is not limited by these embodiments.

[0024] Furthermore, unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which the present invention pertains, and in the event of a conflict, the description in this specification, including the definitions, shall prevail.

[0025] To clearly explain the proposed invention in the drawings, parts unrelated to the description have been omitted, and similar parts throughout the specification have been given similar reference numerals. Furthermore, when a part is described as "comprising" a certain component, this means that, unless specifically stated otherwise, it does not exclude other components but may include additional components. Additionally, the term "part" as described in the specification refers to a single unit or block that performs a specific function.

[0026] In each step, identification codes (1st, 2nd, etc.) are used for convenience of explanation and do not describe the order of the steps; the steps may be performed differently from the specified order unless a specific order is clearly indicated in the context. That is, the steps may be performed in the same order as specified, substantially simultaneously, or in the reverse order.

[0027] The term "extract" as used in the present invention may include the extract itself and any formulation of extract that can be formed using the extract, such as an extract obtained by extracting a raw material, a diluted or concentrated extract of said extract, a dried product obtained by drying said extract, a modified or purified product of said extract, or a mixture thereof. The extract of the present invention may be extracted from natural, hybrid, or variant plants of each of the respective plants, and may also be extracted from plant tissue cultures.

[0028] In the extraction of the raw material of the present invention, the method of extracting the extract is not particularly limited and can be extracted according to methods commonly used in the relevant technical field. Non-limiting examples of the extraction method include hot water extraction, ultrasonic extraction, filtration, reflux extraction, etc., and these may be performed alone or in combination of two or more methods.

[0029] In the present invention, the type of extraction solvent used to extract the raw material is not particularly limited, and any solvent known in the relevant technical field may be used. Non-limiting examples of the extraction solvent may include water; C1 to C4 lower alcohols such as methanol, ethanol, propyl alcohol, butyl alcohol; polyhydric alcohols such as glycerin, butylene glycol, propylene glycol; and hydrocarbon solvents such as ethyl acetate; or mixtures thereof. Preferably, purified water, lower alcohols, 1,3-butylene glycol, and ethyl acetate may be used alone or in a mixture of two or more types.

[0030] In the present invention, the extract obtained by hot water extraction or cold maceration extraction can be used as is after filtering to remove suspended solid particles, such as by using nylon or a freeze-filtration method, or after drying by freeze-drying, hot air drying, spray drying, etc.

[0032] The embodiments and examples of the present invention will be described in detail below. However, the present invention may not be limited to these embodiments, examples, and drawings.

[0034] One aspect of the present invention relates to a cosmetic composition comprising a naturally derived polysaccharide; and an Icelandic moss extract as active ingredients.

[0035] According to the present invention, DNA damage and photodamage to skin cells induced by ultraviolet rays can be effectively inhibited or restored, and structural stability of the skin can be maintained by regulating signaling pathways that cause collagen degradation and skin aging through reduced cell damage caused by reactive oxygen species. In addition, skin inflammatory responses caused by ultraviolet rays can be effectively alleviated through excellent antioxidant and anti-inflammatory effects.

[0036] The cosmetic composition according to the present invention can improve skin barrier lipids to reduce transepidermal water loss (TEWL), strengthen skin barrier function by promoting keratinocyte differentiation and the expression of filaggrin and tight junction proteins, and improve moisturizing power and elasticity. Furthermore, it causes minimal skin irritation and exhibits excellent formulation stability, making it applicable to various formulations, and it can maintain stable quality even during long-term use and storage.

[0037] In one embodiment, the natural-derived polysaccharide is a polymer compound in which monosaccharides are polymerized by glycosidic bonds, and may be extracted or isolated from various natural raw materials such as plants, fungi, seaweed, or microorganisms, or may be manufactured through a fermentation process. The natural-derived polysaccharide is a hydrophilic polymer containing a plurality of hydroxyl groups (-OH) and has excellent water-binding ability, and can form a protective film on the surface of the skin to suppress water evaporation and exhibit a moisturizing effect.

[0038] In one embodiment, the natural-derived polysaccharide may include one or more from the group consisting of plant-derived polysaccharides, fungus-derived polysaccharides, animal-derived polysaccharides, and combinations thereof. For example, the plant-derived polysaccharide may include one or more selected from the group consisting of aloe vera leaf polysaccharide, Centella asiatica polysaccharide, ginseng polysaccharide, ginseng seed polysaccharide, flaxseed polysaccharide, green tea polysaccharide, seaweed polysaccharide, and derivatives thereof, and the fungus-derived polysaccharide may include one or more selected from the group consisting of Phellinus linteus polysaccharide, Ganoderma lucidum polysaccharide, Phellinus linteus polysaccharide, and combinations thereof. Specifically, the natural-derived polysaccharide may include aloe vera leaf polysaccharide and Phellinus linteus polysaccharide.

[0039] In one embodiment, the active ingredient may be included in an amount of about 0.01 to 10 weight percent relative to the total weight of the cosmetic composition. If the content of the active ingredient is less than about 0.01 weight percent, the moisturizing and skin barrier strengthening effects of the aloe vera leaf polysaccharide and white wood ear mushroom polysaccharide and the antioxidant and anti-inflammatory effects of the Icelandic moss extract may not be sufficiently expressed, and thus the effects of inhibiting DNA damage caused by ultraviolet rays and repairing photodamage may be negligible or difficult to achieve at an expected level. In addition, the effects of promoting keratinocyte differentiation and improving the expression of filaggrin and tight junction proteins may also not be sufficiently expressed.

[0040] On the other hand, if the content of the above active ingredient exceeds about 10 weight%, the viscosity of the formulation may increase excessively or gelation may occur due to the increase in the high molecular weight polysaccharide component, which may reduce spreadability and absorption, and the stability of the composition may decrease, leading to problems such as precipitation or separation of layers. In addition, the characteristic color and scent of Icelandic moss extract may appear strongly, which may reduce the user experience, and the possibility of skin irritation due to an excessive amount of active ingredient may increase, so the physical properties as a cosmetic composition may not be desirable.

[0041] As a component of the cosmetic composition according to the present invention, the aloe vera leaf polysaccharide is a high-molecular-weight polysaccharide extracted or isolated from the leaves of Aloe vera, having a structure mainly such as glucomannan, and may be a hydrophilic component having excellent water-binding ability and skin protective film-forming ability. The aloe vera leaf polysaccharide may form a moisture film on the skin surface to inhibit water evaporation and exhibit a moisturizing effect that keeps the skin hydrated, and may protect the skin from external stimuli and provide a skin soothing effect. Furthermore, the aloe vera leaf polysaccharide may exhibit an antioxidant effect that reduces reactive oxygen species generated by ultraviolet rays and an anti-inflammatory effect that alleviates inflammatory reactions, and may contribute to the improvement of DNA damage and photodamage caused by ultraviolet rays by promoting skin cell regeneration and aiding in the recovery of damaged skin tissue. Moreover, by strengthening skin barrier function and inducing normal differentiation of keratinocytes, it may contribute to reducing transepidermal water loss (TEWL) and improving skin elasticity and moisturization capacity.

[0042] In one embodiment, the aloe vera leaf polysaccharide may be included in an amount of about 0.1 to 5 weight percent relative to the total weight of the cosmetic composition. If the content of the aloe vera leaf polysaccharide is less than about 0.1 weight percent, moisturizing, skin soothing, and barrier strengthening effects may not be sufficiently expressed, and the effect of improving skin damage caused by ultraviolet rays may be negligible. On the other hand, if it exceeds about 5 weight percent, the viscosity of the formulation may increase excessively or gelation may occur due to the increase in high-molecular components, which may reduce spreadability and absorption, and the stability of the formulation may decrease, leading to problems such as precipitation or separation of layers.

[0043] As a component of the cosmetic composition according to the present invention, the white wood ear mushroom polysaccharide is a high-molecular-weight polysaccharide extracted or isolated from the white wood ear mushroom (Tremella fuciformis), having a structure including glucuronic acid and mannose, and may be a hydrophilic component having excellent moisture retention ability and viscoelasticity. The white wood ear mushroom polysaccharide can form a uniform moisture protective film on the skin surface to suppress moisture evaporation and provide a long-lasting moisturizing effect to the skin. In addition, the white wood ear mushroom polysaccharide can exhibit an antioxidant effect that removes reactive oxygen species generated by ultraviolet rays and an anti-inflammatory effect that alleviates inflammatory responses, and can contribute to the improvement of DNA damage and photodamage caused by ultraviolet rays by promoting the recovery of damaged skin cells. Furthermore, by stabilizing the lipid structure constituting the skin barrier and promoting the normal differentiation of keratinocytes, it can increase the expression of filaggrin and tight junction proteins, thereby strengthening the skin barrier function and contributing to the reduction of transepidermal water loss (TEWL).

[0044] In one embodiment, the white wood ear mushroom polysaccharide may be included in an amount of about 0.1 to 5 weight percent relative to the total weight of the cosmetic composition. If the content of the white wood ear mushroom polysaccharide is less than about 0.1 weight percent, the moisturizing and skin barrier strengthening effects may not be sufficiently expressed, and the effect of improving skin damage caused by ultraviolet rays may be negligible. On the other hand, if it exceeds about 5 weight percent, due to the characteristics of the high molecular weight polysaccharide, the viscosity of the formulation may increase excessively or gelation may occur, which may reduce spreadability and absorption, and formulation stability may decrease, leading to precipitation or aggregation.

[0045] As a component of the cosmetic composition according to the present invention, Icelandic moss extract is a component extracted or isolated from Icelandic moss (Cetraria islandica) and may include polysaccharides, phenolic compounds, and lichenin, and may be a naturally derived component exhibiting excellent antioxidant and anti-inflammatory activities. The Icelandic moss extract can help soothe skin that has become sensitive due to external stimuli by effectively removing reactive oxygen species generated by exposure to ultraviolet rays and suppressing inflammatory responses within the skin. Furthermore, the Icelandic moss extract can contribute to alleviating skin cell damage induced by ultraviolet rays and inhibiting or restoring DNA damage and photodamage, and can help prevent a decrease in skin elasticity and wrinkle formation by inhibiting signaling pathways that induce collagen degradation. Moreover, by strengthening skin barrier function and inducing normal differentiation of keratinocytes, it can increase the expression of filaggrin and tight junction proteins and reduce transepidermal water loss (TEWL), thereby contributing to improving skin hydration and elasticity.

[0046] In one embodiment, the Icelandic moss extract may be included in an amount of about 0.1 to 5 weight percent relative to the total weight of the cosmetic composition. If the content of the Icelandic moss extract is less than about 0.1 weight percent, the antioxidant and anti-inflammatory effects may not be sufficiently expressed, and the effect of inhibiting and restoring skin damage caused by ultraviolet rays may be negligible. On the other hand, if it exceeds about 5 weight percent, the characteristic color and scent of the extract may appear strongly, which may degrade the sensory characteristics of the formulation, and the stability of the formulation may decrease, leading to precipitation or discoloration.

[0047] In one embodiment, the aloe vera leaf polysaccharide, white wood ear mushroom polysaccharide, and Icelandic moss extract act complementarily to exhibit superior skin improvement effects compared to when used alone. Specifically, the aloe vera leaf polysaccharide and white wood ear mushroom polysaccharide play a role in strengthening skin hydration and barrier function and reducing transepidermal water loss (TEWL) through their excellent water-binding capacity and skin protective film-forming ability, while the Icelandic moss extract can contribute to alleviating skin cell damage by removing reactive oxygen species generated by ultraviolet rays and suppressing inflammatory responses. Accordingly, the combined action of these ingredients effectively inhibits or restores DNA damage and photodamage caused by ultraviolet rays, improves the structural integrity of the skin barrier by promoting keratinocyte differentiation and increasing the expression of filaggrin and tight junction proteins, and consequently exhibits a synergistic effect that simultaneously improves skin hydration and elasticity.

[0048] In one embodiment, the cosmetic composition may further include a plant-derived defensin-like protein. The plant-derived defensin-like protein is a small protein extracted or isolated from a plant, and may be an antimicrobial peptide-family protein having a structure containing cysteine ​​residues and carrying a positive charge to interact with microbial membranes. The plant-derived defensin-like protein exhibits antimicrobial activity, anti-inflammatory effects, and skin barrier regulatory functions, thereby contributing to alleviating the generation of reactive oxygen species and inflammatory responses induced by ultraviolet rays, and promoting the recovery of damaged skin cells. Additionally, it may help reduce transepidermal water loss (TEWL) by inducing the activation and differentiation of keratinocytes and increasing the expression of filaggrin and tight junction proteins to improve the structural integrity of the skin barrier. Specifically, the plant-derived defensin-like protein may include a defensin-like protein extracted or isolated from Pentadiplandra brazzeana.

[0049] In one embodiment, the plant-derived defensin-like protein may be included in an amount of about 0.01 to 3 weight percent relative to the total weight of the cosmetic composition. If the content of the plant-derived defensin-like protein is less than about 0.01 weight percent, the antibacterial, anti-inflammatory, and skin barrier improvement effects may not be sufficiently expressed, and the effects of inhibiting and restoring skin damage caused by ultraviolet rays may be negligible. On the other hand, if the content of the plant-derived defensin-like protein exceeds about 3 weight percent, the formulation stability may be reduced due to the characteristics of the protein component, leading to denaturation or aggregation, and the appearance quality may be degraded due to changes in the viscosity of the formulation or increased turbidity.

[0050] In one embodiment, the cosmetic composition may additionally include Opuntia ficus-indica flower extract. The Opuntia ficus-indica flower extract is a naturally derived ingredient extracted or isolated from the flowers of Opuntia ficus-indica and may include flavonoids, polyphenols, betaine, and various antioxidant substances. As a plant-derived ingredient that survives in dry and strong ultraviolet environments, the Opuntia ficus-indica flower extract possesses the characteristic of protecting cells from external stress and may exhibit an antioxidant effect that effectively removes reactive oxygen species generated by ultraviolet rays, as well as an anti-inflammatory effect that alleviates inflammatory responses.

[0051] In one embodiment, the modified cactus flower extract may be included in an amount of about 0.01 to 3 weight percent relative to the total weight of the cosmetic composition. If the content of the modified cactus flower extract is less than about 0.01 weight percent, the antioxidant and anti-inflammatory effects may not be sufficiently expressed, and the effect of inhibiting and restoring skin damage caused by ultraviolet rays may be negligible. On the other hand, if the content of the modified cactus flower extract exceeds about 3 weight percent, the characteristic color and scent of the extract may appear strongly, which may degrade the sensory characteristics of the formulation, and the stability of the formulation may decrease, leading to precipitation or discoloration.

[0052] In one embodiment, the cosmetic composition may further include an unripe olive fermentation extract. The unripe olive fermentation extract may be an extract prepared through a microbial fermentation process using unripe olive (Olea europaea) fruit as a raw material. Specifically, it may be obtained by a method comprising the steps of: washing and grinding harvested unripe olives; fermenting using lactic acid bacteria, yeast, or a mixture of microorganisms thereof to obtain a fermented product; and filtering and concentrating the fermented product to obtain an unripe olive fermentation extract. Through such a fermentation process, the content of physiologically active components contained in olives, particularly polyphenols such as oleurophenin, may increase or be reduced in molecular weight, thereby improving skin absorption rates.

[0053] Furthermore, the aforementioned unripe olive fermentation extract can exhibit antioxidant effects that effectively eliminate reactive oxygen species generated by ultraviolet rays and anti-inflammatory effects that alleviate inflammatory responses, and can contribute to protecting skin cells and promoting the recovery of damaged skin tissue. Moreover, by strengthening skin barrier function and inducing the normal differentiation of keratinocytes, it can increase the expression of filaggrin and tight junction proteins and reduce transepidermal water loss (TEWL), thereby helping to improve skin hydration and elasticity.

[0054] In one embodiment, the unripe olive fermentation extract may be included in an amount of about 0.01 to 3 weight percent relative to the total weight of the cosmetic composition. If the amount is less than about 0.01 weight percent, the antioxidant and anti-inflammatory effects may not be sufficiently expressed, and the effect of inhibiting and restoring skin damage caused by ultraviolet rays may be negligible. On the other hand, if the amount exceeds about 3 weight percent, the characteristic color and scent of the fermentation extract may appear strongly, which may degrade the sensory characteristics of the formulation, and the stability of the formulation may be reduced, leading to problems such as precipitation, discoloration, or separation of layers.

[0055] In one embodiment, the cosmetic composition may additionally include a moisturizer. The moisturizer is included for the skin moisturizing and skin improvement effects of the cosmetic composition and may include, for example, one or more selected from the group consisting of glycerin, butylene glycol, glycereth-26, hydroxyethyl urea, betaine, and combinations thereof. Preferably, the moisturizer may include glycerin.

[0056] In one embodiment, the moisturizer may be included in the cosmetic composition in an amount of about 1 to 10 weight percent. If the moisturizer is included in an amount of less than about 1 weight percent, the skin moisturizing and skin improvement effects resulting from the inclusion of the moisturizer may not be sufficiently exhibited, and if it exceeds about 10 weight percent, the user experience may decrease.

[0057] In one embodiment, the cosmetic composition may further include one or more additives selected from the group consisting of skin conditioning agents, thickeners, emulsifiers, neutralizing agents, antioxidants, chelating agents, fragrances, and combinations thereof. Specifically, the cosmetic composition may include all of the skin conditioning agent, thickener, emulsifier, neutralizing agent, antioxidant, and chelating agent.

[0058] In one embodiment, the skin conditioning agent may be characterized by comprising one or more selected from the group consisting of cetyl ethylhexanoate, caprylic / capric triglyceride, niacinamide, ethylhexylglycerin, glycereth-25 PCA isostearate, sodium hyaluronate, hydrolyzed hyaluronic acid, and combinations thereof. Preferably, the skin conditioning agent may comprise both cetyl ethylhexanoate and caprylic / capric triglyceride.

[0059] In one embodiment, the skin conditioning agent may be included in the cosmetic composition in an amount of about 1 to 15 weight percent. If the skin conditioning agent is included in an amount of less than about 1 weight percent, the skin soothing, skin regeneration, and skin moisturizing effects resulting from the inclusion of the skin conditioning agent may not be sufficiently exhibited, and if it exceeds about 15 weight percent, the compatibility with other ingredients may be impaired.

[0060] In one embodiment, the thickener is included to control the viscosity of the cosmetic composition and may be characterized by comprising, for example, one or more selected from the group consisting of acrylate / C10-30 alkyl acrylate crosspolymer, carbomer, dimethicone / vinyl dimethicone crosspolymer, dimethicone crosspolymer, vinyl dimethicone, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylmethylcellulose, sodium polyacryloyl dimethyltaurate, and combinations thereof. Preferably, the thickener may comprise carbomer.

[0061] In one embodiment, the thickener may be included in the cosmetic composition in an amount of about 0.1 to 5 weight percent. If the thickener is included in an amount of less than about 0.1 weight percent, the effect resulting from the additional inclusion of the thickener may not be sufficiently exerted, and if it exceeds about 5 weight percent, the formulation may become excessively heavy, increasing the risk of skin trouble or reducing the user experience.

[0062] In one embodiment, the neutralizing agent may be included to adjust the pH of the cosmetic composition and, in particular, to induce the ionization of an acidic polymeric thickener to form and stabilize viscosity. For example, the neutralizing agent may be characterized by comprising one or more selected from the group consisting of tromethamine, sodium hydroxide, potassium hydroxide, ammonium hydroxide, triethanolamine, arginine, and combinations thereof. Preferably, the neutralizing agent may comprise tromethamine.

[0063] In one embodiment, the neutralizing agent may be included in an amount of about 0.01 to 3 weight percent relative to the total weight of the cosmetic composition. If the neutralizing agent is included in an amount of less than about 0.01 weight percent, the pH control effect may not be sufficiently exerted, which may result in reduced stability of the formulation, or the thickener may not be sufficiently activated, which may result in insufficient viscosity formation. On the other hand, if the neutralizing agent exceeds about 3 weight percent, the pH may rise excessively, causing skin irritation, and the viscosity of the formulation may increase excessively or become unstable, which may result in reduced usability.

[0064] In one embodiment, the antioxidant may be characterized by comprising one or more selected from the group consisting of tocopherol, tocopheryl acetate, ascorbic acid, ascorbyl palmitate, butylhydroxytoluene, butylhydroxyanisole, ferulic acid, ubiquinone, rosemary extract, and combinations thereof. Preferably, the antioxidant may comprise tocopherol.

[0065] In one embodiment, the antioxidant may be included in an amount of about 0.1 to 5 weight percent relative to the total weight of the cosmetic composition. If included in an amount less than about 0.1 weight percent, the antioxidant effect may be insufficient, which may reduce the storage stability and formulation stability of the cosmetic composition being manufactured, and if included in an amount exceeding about 5 weight percent, discoloration of the formulation, odor, or skin irritation may occur.

[0066] In one embodiment, the chelating agent may be characterized by comprising one or more selected from the group consisting of disodium EDTA, sodium citrate, citric acid, sodium diphosphate, sodium triphosphate, nitrilotrimethylenephosphoic acid, ethylenediaminetetraacetate, and combinations thereof. Preferably, the chelating agent may comprise disodium EDTA.

[0067] In one embodiment, the chelating agent may be included in the cosmetic composition in an amount of about 0.01 to 3 weight percent. If the amount is less than about 0.01 weight percent, the stability of the cosmetic composition being prepared may decrease, and if the amount exceeds about 3 weight percent, skin irritation may occur.

[0068] In one embodiment, the emulsifier is added to facilitate mixing of the components included in the cosmetic composition and may be characterized by comprising, for example, one or more selected from the group consisting of glyceryl stearate, polyglyceryl-3 methylglucose distearate, hydrogenated lecithin, polysorbate 60, glyceryl caprylate, caprylyl / capryl glucoside, and combinations thereof. Preferably, the emulsifier may include glyceryl stearate and polyglyceryl-3 methylglucose distearate.

[0069] In one embodiment, the emulsifier may be included in the cosmetic composition in an amount of about 0.1 to 5 weight percent. If the emulsifier is included in an amount of less than about 0.1 weight percent, separation of layers may occur or storage stability may decrease, and if it exceeds about 5 weight percent, excessive foaming may occur.

[0070] In one embodiment, the cosmetic composition may comprise about 70 to 90 weight% of a solvent, about 0.1 to 5 weight% of an emulsifier, about 0.1 to 5 weight% of an aloe vera leaf polysaccharide, about 0.1 to 5 weight% of a white wood ear mushroom polysaccharide, about 0.1 to 5 weight% of an Icelandic moss extract, about 1 to 15 weight% of a skin conditioning agent, about 0.01 to 3 weight% of a chelating agent, about 1 to 10 weight% of a moisturizer, about 0.1 to 5 weight% of a thickening agent, and about 0.1 to 5 weight% of an antioxidant.

[0071] In one embodiment, the solvent is not limited as long as it facilitates mixing the components included in the cosmetic composition, and may include, for example, one or more selected from the group consisting of purified water, 1,2-hexanediol, propylene glycol, butylene glycol, ethanol, and combinations thereof. Preferably, the solvent may include purified water or 1,2-hexanediol.

[0072] In one embodiment, if the solvent is included in an amount of less than about 70 weight%, the components may not be sufficiently dissolved, and if it exceeds about 90 weight%, the pharmacological effect of the cosmetic composition being prepared may not be sufficiently exerted.

[0073] In one embodiment, the cosmetic composition may be characterized as being for improving the skin barrier and / or repairing skin damage.

[0074] In one embodiment, the cosmetic composition may additionally include, but is not limited to, ingredients typically required to manufacture a cosmetic composition in addition to the described ingredients.

[0075] In one embodiment, the cosmetic composition may be formulated into one or more selected from the group consisting of toner, lotion, essence, gel, mask pack, powder, balm, cleanser, and combinations thereof.

[0076] In one embodiment, the cosmetic composition may include any substance selected according to the formulation or intended use of the cosmetic. For example, purified water (solvent), oil, surfactant, humectant, higher alcohol, thickening agent, chelating agent, colorant, fatty acid, antioxidant, preservative, wax, pH adjuster, fragrance, etc. may be added, but are not limited thereto.

[0077] In one embodiment, when the formulation of the cosmetic composition is a paste, cream, or gel, the carrier component may include, but is not limited to, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide.

[0078] In one embodiment, when the formulation of the cosmetic composition is a powder or a spray, it may include lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder as a carrier component, and in particular, when it is a spray, it may additionally include a propellant such as chlorofluorohydrocarbon, propane / butane, or dimethyl ether, but is not limited thereto.

[0079] In one embodiment, when the formulation of the cosmetic composition is a solution or emulsion such as a toner, the carrier component may include a solvent, a solubilizing agent, or an emulsifying agent, and may include, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, or fatty acid ester of sorbitan, but is not limited thereto.

[0080] In one embodiment, when the formulation of the cosmetic composition is a suspension such as an essence or ampoule, the carrier component may include a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracanth, etc., but is not limited thereto.

[0081] In one embodiment, when the formulation of the cosmetic composition is a surfactant-containing cleansing agent, the carrier component may include, but is not limited to, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester.

[0082] In one embodiment, the effect described above is exerted through the organic interaction of all the components described above, and the effect may not be achieved if the active ingredient is included selectively.

[0084] Hereinafter, the structure of the present invention and the resulting effects are to be explained in more detail through specific embodiments and comparative examples. However, these embodiments are intended to explain the present invention more specifically, and the scope of the present invention is not limited to these embodiments.

[0086] [Examples 1 to 3]

[0087] A cosmetic composition in the form of a cream was prepared by mixing the components listed in Table 1 below. Based on the weight ratio of each component, the prepared compositions were named Example 1, Example 2, and Example 3, respectively.

[0088] Composition (weight%) Example 1 Example 2 Example 3 purified water 80.60 79.85 79.35 Glyceryl stearate 2.00 2.00 2.00 Polyglyceryl-3 methylglucose distearate 1.50 1.50 1.50 Aloe vera leaf polysaccharide 0.50 0.50 0.50 White wood ear mushroom polysaccharide 0.50 0.50 0.50 Icelandic moss extract 1.00 1.00 1.00 Cetylethylhexanoate 3.00 3.00 3.00 Caprylic / Capric Triglyceride 5.00 5.00 5.00 Defensin-like protein 0.00 0.50 0.50 Changju cactus flower extract 0.00 0.25 0.25 Fermented unripe olive extract 0.00 0.00 0.50 Chelating agent (disodium EDTA) 0.10 0.10 0.10 moisturizer (glycerin) 5.00 5.00 5.00 Thickener (Carbomer) 0.30 0.30 0.30 Neutralizing agent (tromethamine) 0.20 0.20 0.20 Antioxidant (tocopherol) 0.30 0.30 0.30 total 100.00 100.00 100.00

[0090] [Comparative Example]

[0091] A cosmetic composition of a comparative example was prepared by the same method as in Examples 1 to 3 above, but by mixing only some of the components as shown in Table 2 below.

[0092] Composition (weight%) Comparative Example 1 Comparative Example 2 Comparative Example 3 purified water 82.10 82.10 81.60 Glyceryl stearate 2.00 2.00 2.00 Polyglyceryl-3 methylglucose distearate 1.50 1.50 1.50 Aloe vera leaf polysaccharide 0.50 0.00 0.00 White wood ear mushroom polysaccharide 0.00 0.50 0.00 Icelandic moss extract 0.00 0.00 1.00 Cetylethylhexanoate 3.00 3.00 3.00 Caprylic / Capric Triglyceride 5.00 5.00 5.00 Chelating agent (disodium EDTA) 0.10 0.10 0.10 moisturizer (glycerin) 5.00 5.00 5.00 Thickener (Carbomer) 0.30 0.30 0.30 Neutralizing agent (tromethamine) 0.20 0.20 0.20 Antioxidant (tocopherol) 0.30 0.30 0.30 total 100.00 100.00 100.00

[0094] [Experimental Example 1: Inhibition of DNA damage caused by ultraviolet rays and effect of photodamage recovery]

[0095] To confirm the effects of the cosmetic compositions prepared in the above examples and comparative examples on inhibiting UV-induced DNA damage and repairing photodamage, experiments were conducted using human-derived keratinocytes (HaCaT) or human dermal fibroblasts (HDF).

[0096] Specifically, cells were seeded into a 96-well or 6-well plate and stabilized for 24 hours, after which they were divided into groups treated with the compositions of Examples 1 to 3 and groups treated with the Comparative Example. Subsequently, each group was treated with a sample at a predetermined concentration and then irradiated with UVB to induce an ultraviolet damage model. After UVB irradiation, the cells were cultured for a certain period of time, and then the degree of DNA damage was evaluated.

[0097] The degree of DNA damage was determined by measuring the amount of cyclobutane pyrimidine dimer (CPD) or 8-hydroxy-2'-deoxyguanosine (8-OHdG) formed by UV irradiation. The measurement results are shown in Table 3 below.

[0098] division 8-OHdG relative value (% of control) negative control (no treatment, no investigation) 100 ± 5 Example 1 150 ± 5 Example 2 130 ± 5 Example 3 115 ± 4 Comparative Example 1 180 ± 7 Comparative Example 2 170 ± 6 Comparative Example 3 160 ± 6

[0100] As shown in Table 3 above, in Comparative Examples 1 to 3 irradiated with UVB, the amount of 8-OHdG, a DNA damage marker, was significantly increased, whereas in Examples 1 to 3 according to the present invention, the amount of the DNA damage marker was significantly decreased, confirming that excellent photodamage inhibition and recovery effects were exhibited.

[0102] [Experimental Example 2: Measurement of Antioxidant Effect]

[0103] Samples were prepared by diluting each of the cosmetic compositions prepared in the above examples and comparative examples with 10 times the amount of distilled water, and the DPPH (1,1-diphenyl-2-picrylhydroxy drazyl, Sigma-Aldrich, St. Louis, MO, USA) radical scavenging activity of each sample was measured by the modified Blois (1958) method. The measurement method is as follows: 1.5 × 10⁻⁶ per 100 μL of each diluted sample -4 100 μL of M DPPH solution was added and left to stand in a dark room at room temperature for 30 minutes, after which the absorbance was measured at 520 nm, and the results of the measured DPPH radical scavenging activity are shown in Table 4.

[0104] division Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Comparative Example 3 DPPH radical scavenging activity (%) 64.45 70.11 82.12 58.74 60.45 57.46

[0106] As shown in Table 4 above, it was confirmed that the cosmetic composition of the example exhibited improved DPPH radical scavenging activity compared to the comparative example.

[0108] [Experimental Example 3: Effect of improving skin barrier function]

[0109] In order to confirm the skin barrier function improvement effect of the cosmetic composition according to the above examples and comparative examples, an experiment was conducted by measuring transepidermal water loss (TEWL).

[0110] Specifically, 20 healthy adult subjects were tested, and a certain amount of the compositions of Examples 1 to 3 and Comparative Examples 1 to 3 was applied to the forearms of each subject, and they were instructed to use them twice a day for 4 weeks. The TEWL values ​​of the skin were measured before use, 2 weeks after use, and 4 weeks after use.

[0111] TEWL measurements were performed in a standardized environment (temperature 22±2℃, relative humidity 50±5%), and the measurement results are shown in Table 5 below.

[0112] division Before use 2 weeks later 4 weeks later Comparative Example 1 18.5 ± 1.2 17.8 ± 1.1 17.2 ± 1.0 Comparative Example 2 18.3 ± 1.3 17.5 ± 1.2 17.0 ± 1.1 Comparative Example 3 18.6 ± 1.1 17.6 ± 1.0 17.1 ± 1.0 Example 1 18.4 ± 1.2 16.5 ± 1.1 15.2 ± 1.0 Example 2 18.5 ± 1.3 15.8 ± 1.0 14.0 ± 0.9 Example 3 18.6 ± 1.2 15.0 ± 0.9 13.2 ± 0.8

[0114] As shown in Table 5 above, when the cosmetic compositions of Examples 1 to 3 according to the present invention were used, it was confirmed that the skin barrier function was effectively improved by significantly reducing transepidermal water loss (TEWL) compared to the comparative example.

[0116] In this specification, only a few examples among the various embodiments performed by the inventors are described; however, the technical concept of the present invention is not limited or restricted thereto, and it is obvious that it can be modified and implemented in various ways by those skilled in the art.

Claims

Claim 1 70 to 90 wt% purified water; 0.1 to 5 wt% aloe vera leaf polysaccharide; 0.1 to 5 wt% white wood ear mushroom polysaccharide; 0.01 to 3 wt% defensin-like protein; 0.01 to 3 wt% modified cactus flower extract; 1 to 10 wt% glycerin; 1 to 15 wt% skin conditioning agent comprising cetyl ethylhexanoate and caprylic / capric triglyceride; 0.1 to 5 wt% carbomer; 0.01 to 3 wt% tromethamine; 0.1 to 5 wt% tocopherol; 0.01 to 3 wt% disodium EDTA; 0.1 to 5 wt% emulsifier comprising glyceryl stearate and polyglyceryl-3 methylglucose distearate; A cosmetic composition comprising 0.1 to 5 weight% of Icelandic moss extract. Claim 2 delete Claim 3 delete Claim 4 delete Claim 5 A cosmetic composition according to claim 1, characterized in that the cosmetic composition is a formulation selected from the group consisting of toner, lotion, essence, gel, mask pack, powder, balm, cleanser, and combinations thereof.