Wound treatment film and its manufacturing method

Abstract

A wound healing film comprising a gellan gum and a chitosan layer containing capsules containing sappanwood extract and vitamin leaf extract is provided.

Description

Technology Field

[0001] The present invention relates to a film for wound treatment and a method for manufacturing the same. Background Technology

[0002] The skin is an important organ that occupies the largest surface area in our body, serving not only to protect the human body from various harmful environments such as external microorganisms, ultraviolet rays, and chemicals, but also to prevent dehydration by inhibiting water evaporation and regulate body temperature.

[0003] As industrial society develops, the likelihood of skin damage caused by various factors such as severe burns, trauma, wounds, pressure ulcers, and skin diseases is increasing, and wounds that cannot be sutured in the first place may leave severe defects that necessitate skin grafting.

[0004] A wound refers to a condition in which the continuity of tissue is disrupted by external pressure. Skin wounds frequently occur due to various causes, and their healing requires a complex process involving the interaction of various molecules, such as cytokines and growth factors, that induce the growth and regeneration of the injured tissue. Furthermore, if a wound remains open for a long time, secondary complications such as bacterial and viral infections may arise, and it can cause pain or inconvenience in daily life during the treatment period; therefore, it is crucial to ensure that wounds heal early once they occur. Consequently, various types of wound dressings are widely used to protect the skin from bacterial infection and dehydration at the wound site.

[0005] In addition, as the side effects of synthetic drugs for wound healing, cavity prevention, and oral regeneration have become apparent, various products utilizing natural drugs are being researched.

[0006] Antioxidant, antibacterial, and anti-inflammatory effects have been reported for vitamin leaf extracts. Despite the potential for such physiological activity, they are currently applied only in functional foods and cosmetics. The nutritional value of the water-soluble vitamins contained in vitamin leaves is superior to that of synthetic vitamin C due to the inclusion of minerals, organic acids, and fatty acids.

[0007] Sappanwood extract is a medicinal herb listed in the *Donguibogam* and the *Pharmacopoeia of the Republic of Korea*, and is the heartwood of *Caesalpinia sappan Linne* (Leguminosae, family Leguminosae).

[0008] In clinical studies, various results regarding antibacterial, anticancer, antioxidant, anti-inflammatory, and cell proliferation inhibition effects have been reported. Currently, Sappanwood extract is utilized as a main ingredient in over-the-counter medicines, making it an extract with wide-ranging utility.

[0009] Most cases of periodontitis are caused by inflammation of the gums (gingivitis) and the long-term accumulation of plaque (a thin film of material composed primarily of bacteria, saliva, food debris, and dead cells that constantly deposit on the teeth) and tartar (hardened plaque) on the teeth and gums. Pockets form between the teeth and gums, extending down to the area between the tooth roots and the underlying bone. When plaque builds up in this bone in a low-oxygen environment, it promotes the proliferation of aggressive bacterial forms in individuals with immune system vulnerabilities to periodontitis. Plaque and bacteria cause chronic inflammation that damages the tissues and bone that hold the teeth in place. If the disease persists, too much bone is eventually lost, leading to painful loose teeth and gum recession.

[0010] To prevent early-stage gingivitis, it is necessary to brush thoroughly using dental floss and interdental brushes. If gingivitis is in its early stages, basic hygiene management and the use of mouthwash are essential.

[0011] In particular, the main ingredients used as mouthwashes include cetylpyridinium chloride hydrate (CPC), essential oils, sodium fluoride (NaF), and chlorhexidine gluconate. Hexamedine used in dentistry refers to a dental oral medicine rather than a mouthwash, and it is a 0.12% concentration chlorhexidine gluconate solution, which is the best among the Gargrin series, to the point where brushing is unnecessary.

[0012] Hexamedine is an over-the-counter drug that can be easily purchased at pharmacies. Its drawback is that it causes extremely severe staining. Using just one large bottle for two to four weeks results in teeth that look like those of a smoker who has smoked for several years. Since the liquid form of Hexamedine requires the inconvenience of dilution, technological development is needed. Prior art literature

[0013] Korean Published Patent No. 10-2022-0078805, Korean Registered Patent No. 10-2553868 The problem to be solved

[0014] The present invention aims to provide a wound treatment film having excellent effects on inflammation control and wound regeneration. means of solving the problem

[0015] In order to achieve the above objectives, according to one embodiment of the present invention, a wound healing film comprising a gellan gum and a chitosan layer containing capsules containing sappanwood extract and vitamin leaf extract is provided.

[0016] According to one embodiment of the present invention, the sappanwood extract and vitamin leaf extract may be alcohol extracts.

[0017] According to one embodiment of the present invention, the capsule may be a complex of sappanwood extract and vitamin leaf extract-cyclodextrin inclusion.

[0018] According to one embodiment of the present invention, a method for manufacturing a wound healing film is provided, comprising the steps of heat drying and freeze-drying a coated capsule containing sappanwood extract and vitamin leaf extract, gellan gum, and chitosan solution.

[0019] According to one embodiment of the present invention, the method may further include the step of mixing a capsule solution with gellan gum and a chitosan solution to prepare the capsule containing the sappanwood extract and vitamin leaf extract, the gellan gum, and the chitosan solution.

[0020] According to one embodiment of the present invention, the method further includes the step of preparing the gellan gum and chitosan solutions by mixing the gellan gum solution and the chitosan solution, and the solvent of the chitosan solution may be a cation-containing solvent.

[0021] According to one embodiment of the present invention, the temperature of the gellan gum solution and chitosan solution mixed may be 60 to 90 ℃.

[0022] According to one embodiment of the present invention, the concentrations of gellan gum and chitosan in the solution may each be 0.5 to 3 weight percent.

[0023] According to one embodiment of the present invention, the weight ratio of gellan gum to chitosan in the solution may be 7:3 to 3:7.

[0024] According to one embodiment of the present invention, the concentration of the capsule may be 1 to 3 weight percent. Effects of the invention

[0025] The wound healing film of the present invention comprises a gellan gum and a chitosan layer containing capsules loaded with sappanwood extract and vitamin leaf extract, thereby exhibiting excellent effects in controlling inflammation and wound regeneration.

[0026] The wound healing film of the present invention comprises a capsule containing sappanwood extract and vitamin leaf extract, thereby enabling sustained release of the extract without change in shape.

[0027] The wound healing film of the present invention has a denser structure and improved physical properties. Brief explanation of the drawing

[0028] Figure 1 is the GC-MS result of the Sappanwood extract of Preparation Example 1. Figure 2 is the GC-MS result of the vitamin leaf extract of Preparation Example 2. Figure 3 is a photograph of the results of the bacterial inhibition experiment of the extracts of Preparation Example 1 and Preparation Example 2. Figure 4 shows the results of confirming the cell viability of the complex extract mixed with each extract of Preparation Example 1 and Preparation Example 2. Figure 5 shows the cell motility results of a complex extract mixed with each extract of Preparation Example 1 and Preparation Example 2. Figure 6 is a photograph of the sappanwood and vitamin leaves used in Preparation Example 3. Figure 7 is a photograph of the gamma dextrin used in Preparation Example 3. Figure 8 shows the antioxidant capacity results of Preparation Example 3. Figure 9 shows the SEM and particle distribution results of Preparation Example 3. Figure 10 is a photograph of the film result of Preparation Example 4. Figure 11 shows the tensile strength measurement results of Manufacturing Example 4. Figure 12 is a photograph of the film of Example 1. Figure 13 shows the swelling degree results according to the GC / GEL composition. Figure 14 is a photograph of the film results according to the GC / GEL composition. Figure 15 is a film photograph of Comparative Example 3. Figure 16 is a film photograph of Comparative Example 4. Specific details for implementing the invention

[0029] The present invention will be described in detail below. Prior to this, terms or words used in this specification and claims should not be interpreted as being limited to their ordinary or dictionary meanings, but should be interpreted in a meaning and concept consistent with the technical spirit of the present invention, based on the principle that the inventor can appropriately define the concept of the terms to best describe his invention. Accordingly, the configurations described in the embodiments described in this specification are merely one preferred embodiment of the present invention and do not represent all of the technical spirit of the present invention; therefore, it should be understood that various equivalents and modifications capable of replacing them may exist at the time of filing this application.

[0030] According to one embodiment of the present invention, a wound healing film comprising a gellan gum and a chitosan layer containing capsules containing sappanwood extract and vitamin leaf extract is provided.

[0031] Sappanwood extract is pathogenic microorganisms S. mutans It is a substance that has excellent effects in inhibiting the proliferation of major causative bacteria, and can be utilized for inflammation control and regeneration.

[0032] Sappanwood extract may be an alcohol extract of sappanwood. For example, it can be prepared by thoroughly washing and drying sappanwood, extracting it with an alcohol such as ethanol, evaporating the alcohol using a vacuum evaporator, and then freeze-drying it.

[0033] Sappanwood extract may contain polyphenol compounds, such as gamma-sitosterol, carnosol, vitamin E, ambrettolide, etc.

[0034] Vitamin leaf extract contains a large amount of vitamin C and has strong water affinity, making it excellent for stopping bleeding and protecting wounds.

[0035] Vitamin leaf extract may be an alcohol extract. For example, it can be prepared by naturally drying thoroughly washed vitamin leaves, extracting them with an alcohol such as ethanol, evaporating the alcohol using a vacuum evaporator, and then freeze-drying the resulting product.

[0036] Vitamin leaf extract may contain polyphenol compounds, such as palmitic acid, linolelaidic acid, 6-octadecenoic acid, oleic acid, 1-tricosene, etc.

[0037] Capsules containing sappanwood extract and vitamin leaf extract may be capsules in which sappanwood extract and vitamin leaf extract are encapsulated individually or in a mixture.

[0038] When a film is manufactured without encapsulating the sappanwood extract and vitamin leaf extract, if the total of the sappanwood extract and vitamin leaf extract is 1% by weight or more, cracks occur in the film, resulting in a problem of not being able to contain a large amount of active substances.

[0039] Accordingly, the inventors have been able to include more than 1% by weight of an active substance in a film by encapsulating and applying sappanwood extract and vitamin leaf extract, thereby providing excellent effects in controlling inflammation and wound regeneration.

[0040] The encapsulation may involve loading Sappanwood extract and vitamin leaf extract inside an amphiphilic material that is hydrophilic on the outside and hydrophobic on the inside.

[0041] The amphiphilic substance is capable of carrying hydrophobic physiologically active substances, such as sappanwood extract and vitamin leaf extract, and may be, for example, a cyclodextrin. The cyclodextrin may be, for example, a gamma cyclodextrin.

[0042] A capsule solution can be prepared by mixing an amphiphilic substance, sappanwood extract, and vitamin leaf extract in a solvent, and the capsule solution can be powdered through freeze-drying.

[0043] Powdered capsules can be spherical.

[0044] The powdered capsule may have an average diameter of 300 to 400 nm.

[0045] The loading efficiency of the extract within the capsule can be 80% or higher.

[0046] The capsule may be a sappanwood extract and vitamin leaf extract-cyclodextrin inclusion complex.

[0047] In addition, a method for manufacturing a wound healing film is provided, comprising the steps of heat drying and freeze-drying a coated capsule containing sappanwood extract and vitamin leaf extract, gellan gum, and chitosan solution.

[0048] To avoid repetition, the aforementioned capsules containing sappanwood extract and vitamin leaf extract are omitted from the following description, but they can be applied in the same way to the method of manufacturing a wound healing film.

[0049] The method of the present invention may further include the step of preparing gellan gum and chitosan solutions by mixing a gellan gum solution and a chitosan solution.

[0050] In the present invention, gellan gum is a grayish-white, tasteless, and odorless powdered substance obtained by purifying and fermenting carbohydrates using Pseudomonas elodea, which is isolated from a water plant of the genus Elodea, and then purifying, drying, and grinding the high-molecular-weight polysaccharide gum substance using sopropanol as a solvent.

[0051] Gellan gum can be a heteropolysaccharide composed of, for example, rhamnose, glucuronic acid, and glucose in a 1:1:2 ratio.

[0052] Gellan gum is a type of thickening stabilizer, and as a polysaccharide produced by the fermentation of microorganisms, such as curdlan, gellan gum, and xanthan gum, it can be used as an additive in foods and pharmaceuticals.

[0053] Chitosan is a linear polysaccharide composed of D-glucosamine and N-acetylglucosamine. For example, it can be obtained by treating the shells of crustaceans such as snow crabs and shrimp with sodium hydroxide.

[0054] The gellan gum and chitosan solution refers to a solution containing gellan gum and chitosan, and preferably, the gellan gum solution and the chitosan solution may be prepared separately and then mixed.

[0055] The gellan gum solution may be prepared by dissolving a certain proportion of gellan gum in a solvent until it becomes a clear and transparent solution.

[0056] The solvent for the gellan gum solution may be any solvent capable of dissolving gellan gum, without restriction, and may be, for example, water.

[0057] The chitosan solution may be prepared by dissolving chitosan in a solvent until it becomes a clear and transparent solution.

[0058] The solvent of the chitosan solution can be any solvent capable of dissolving chitosan without limitation, and water-soluble solvents such as water, dimethyl sulfoxide (DMSO), methanol, ethanol, butanol, propanol, glycerin, propylene glycol, butylene glycol, and various other alcohols can be used.

[0059] The solvent of the chitosan solution may be a cation-containing solvent. Since cations are capable of crosslinking gellan gum, and gellan gum becomes immediately crosslinked if the solvent of the gellan gum solution contains cations, the solvent of the chitosan solution may contain cations.

[0060] The cation can be a monovalent or divalent cation, for example, Na + , k+ , Ca 2+ , Mg 2+ It could be the back.

[0061] Cation-containing solvents may include, for example, Dulbecco's phosphate-buffered saline (D-PBS), but are not limited thereto.

[0062] The temperature of the gellan gum solution and chitosan solution being mixed is a temperature at which the gellan gum and chitosan are completely dissolved into a clear and transparent solution, and may be, for example, 60 to 90°C. If the temperature is below the above range, the solution may solidify, and if it exceeds the above range, it may undergo thermal deformation.

[0063] The concentrations of gellan gum and chitosan in the mixed gellan gum and chitosan solution may each be 0.5 to 3 weight%. If the concentration of each is less than 0.5 weight%, it may be insufficient for film formation, and if it exceeds 3 weight%, the solution becomes high-viscosity, making film formation difficult, and there is a problem of foreign body sensation when applied to the oral cavity, etc. due to the increased thickness of the manufactured film.

[0064] The total concentration of gellan gum and chitosan in the mixed gellan gum and chitosan solution may be 2% by weight or more for film formation. If the total concentration is less than 2% by weight, the film may not be formed sufficiently and tearing may occur.

[0065] The weight ratio of gellan gum to chitosan in the above gellan gum and chitosan solution may be 7:3 to 3:7 to form a film and secure physical properties, and may be, for example, 6:4 to 4:6.

[0066] If the weight ratio of gellan gum exceeds 70%, the film thickness becomes thin and tears, and if the weight ratio of chitosan exceeds 70%, the thickness becomes thick and the shape becomes uneven and non-uniform.

[0067] The mixed gellan gum and chitosan solution can be mixed according to the aforementioned ratio and then homogenized for 3 to 10 seconds.

[0068] The method of the present invention may further include the step of mixing a capsule solution with a gellan gum and a chitosan solution to prepare the capsule containing the sappanwood extract and vitamin leaf extract, the gellan gum, and the chitosan solution.

[0069] The capsule solution can be prepared by mixing the extract solution and the carrier solution.

[0070] The extract solution may be prepared by adding sappanwood extract and vitamin leaf extract to a solvent and then stirring to dissolve them.

[0071] The solvent of the extract solution may be alcohol.

[0072] The support solution may be prepared by adding an amphiphilic substance, which is hydrophilic on the outside and hydrophobic on the inside, to a solvent and stirring it.

[0073] The solvent of the support solution can be distilled water.

[0074] The concentration of the capsule is 3 weight% or less, and preferably 1 to 3 weight%. If the concentration of the capsule exceeds 3 weight%, it may be difficult to form a film.

[0075] It may further include a step of homogenizing the mixed gellan gum and chitosan solution and the capsule solution, and then applying it.

[0076] The applied solution can be heat-dried to form a dense structure.

[0077] The heat drying temperature can be, for example, 40 to 80°C.

[0078] The heat drying time can be, for example, 3 to 9 hours.

[0079] The heat drying conditions can be appropriately adjusted to have an appropriate moisture content depending on the thickness of the applied solution, the solvent content, the application area, etc.

[0080] The heat-dried solution can be freeze-dried, and any technology and conditions applicable in the relevant technical field can be applied without limitation. As a non-limiting example, the gellan gum and chitosan solution, which has been heat-dried at a temperature of minus 10°C or lower, can be dried by freezing it and then lowering the surrounding pressure to directly sublimate it from the solid phase to the gas phase.

[0082] The present invention will be explained in more detail based on the following examples. However, the following examples are merely illustrative of the present invention and do not limit the present invention.

[0084] Meanwhile, in the following examples and drawings, the sappanwood extract may be used in combination with Biancaea sappan extract and Be. The vitamin leaf extract may be used in combination with Hippophae rhamniodes extract and He. Chitosan may be used in combination with GC, and gellan gum may be used in combination with GEL.

[0086] Preparation Example 1 - Preparation of Sappanwood Extract and Evaluation of Components

[0087] After naturally drying 100g of thoroughly washed wood from sappanwood, extract it with 30% ethanol, evaporate the organic solvent using a vacuum evaporator, and freeze-dry to obtain a sappanwood extract (yield 18%). To identify the major components, measurements were performed using GC-MS. Figure 1 shows the GC-MS results of the sappanwood extract from Preparation Example 1. Referring to Figure 1, various polyphenol compounds were detected as major components, and in particular, Gamma-Sitosterol, carnosol, Vitamin E, and Ambrettolide were identified.

[0089] Preparation Example 2 - Evaluation of Vitamin Leaf Extract and Components

[0090] 100g of thoroughly washed vitamin leaves were naturally dried, extracted with 10% ethanol, and the organic solvent was evaporated using a vacuum evaporator, followed by freeze-drying to obtain a vitamin leaf extract (yield 25%). To identify the main components, measurements were performed using GC-MS. Figure 2 shows the GC-MS results of the vitamin leaf extract from Preparation Example 2. Referring to Figure 2, polyphenol compounds were detected as the main components, and in particular, Palmitic acid, Linolelaidic acid, 6-0 ctadecenoic acid, Oleic acid, and 1-Tricosene were identified.

[0092] Evaluation Example 1- S.mutans and C.albicans Bacterial inhibition experiment

[0093] extracts with pharmacological properties S.mutans and C.albicans Antimicrobial activity test against bacteria was performed using the agar medium diffusion method (paper disc diffusion) of the extracts of Preparation Example 1 and Preparation Example 2 S.mutans and C.albicans The antibacterial activity against bacteria was measured. S.mutans and C.albicans The bacteria were activated by subculturing multiple times and 10 in BHI liquid medium and LB medium 7 100 μL of bacteria diluted to CFU ml was plated onto solid media. Each extract was used at a concentration of 10 mg / ml. 50 μL of each experimental group was spotted onto a paper disc (8 mm, Whatman, USA), dried, and then placed on the solid media plated with the experimental strains. The discs were incubated at 37°C for 24 hours, and the diameter of the clear zone around the disc was measured. According to each concentration S.mutans The liquid medium in which the sample was co-cultured was inoculated onto a solid medium to measure the zone of inhibition.

[0094] Figure 3 is a photograph of the results of the bacterial inhibition experiment of the extracts of Preparation Example 1 and Preparation Example 2. Referring to Figure 3, at a concentration of 10 mg / ml of each extract, the sample for mutans was 10 mm and the sample for Be was 16 mm, and for C. albicans, the sample was 12 mm and the sample for Be was 20 mm. According to the susceptibility reading criteria, a strong antibacterial effect was observed, and high antibacterial activity was observed for both strains.

[0096] Evaluation Example 2 - Evaluation of Cell Viability

[0097] An MTT reduction assay was performed to confirm the cell viability of the L929 cell line. The cell line was plated in a 96-well plate at a density of 1 x 10⁶ 5 100 μL aliquots were dispensed at a concentration of cells / mL and cultured for 24 hours in a 37°C, 5% CO2 incubator. Subsequently, the extracts were treated at concentrations of 125, 250, 500, and 1000 ppm, respectively, and cultured for another 24 hours. 1 μL of MTT (5 μg / mL) solution dissolved in PBS buffer was added to each well, and the mixture was reacted for another 1 hour. After confirming formazan formation, the medium was completely removed. 100 μL of DMSO was added to dissolve the formazan formed at the bottom of the wells. Absorbance was then measured at 540 nm using an ELISA reader (Model 680, BioRad, USA) to represent the relative cell viability compared to control cells cultured without extract treatment, which were set to 100%.

[0098] Figure 4 shows the results of confirming the cell viability of the complex extract mixed with the extracts of Preparation Example 1 and Preparation Example 2. Referring to Figure 4, a cell viability of 80% or more was confirmed in all complex extracts mixed with the extracts of Preparation Example 1 and Preparation Example 2.

[0100] Evaluation Example 3 - Evaluation of Cell Mobility Promotion

[0101] When a cell monolayer is scratched, surrounding cells repair the damaged area through cell proliferation and migration. In other words, cell migration, along with cell proliferation, is of great significance in the wound healing process. In this invention, to evaluate whether the experimental substance promotes cell migration, a void space was created by scratching a dermal fibroblast cell monolayer with a p200 pipet tip, and a serum-free medium was added and cultured for 24 hours; the degree of cell migration in the experimental group was compared with the control group using an inverted micoscope.

[0102] Figure 5 illustrates the cell motility results of the complex extract obtained by mixing the extracts of Preparation Example 1 and Preparation Example 2. Referring to Figure 5, it was observed that cell motility was more active in the group extracted by mixing the two extracts than in the group treated with each extract.

[0104] Preparation Example 3 - Encapsulation of extract

[0105] Vitamin leaf extract (Hippophae rhamniodes extract, He) and sappanwood extract (Biancaea sappan extract, Be) were mixed with 30% ethanol and encapsulated in gamma-cyclodextrin (Wacker Chemie AG, Iowa, USA). Gamma-cyclodextrin (1 g) was added to 50 ml of distilled water and stirred for 1 hour to dissolve. Sappanwood extract (1.0 g) and vitamin leaf extract (1.0 g) were added to 5% ethanol and stirred for 1 hour to dissolve. Each solution was mixed and homogenized using an Ultra Homogenizer (M-11OS Microfluidizer, Microfluidics Co. Newton, USA) at 20,000 rpm for 15 minutes to prepare capsules. At this time, Fig. 6 is a photograph of the sappanwood and vitamin leaf used in Preparation Example 3, and Fig. 7 is a photograph of the gamma-cyclodextrin used in Preparation Example 3.

[0106] The morphology and particle size of the capsules were observed using a HITACHI S-4700 scanning electron microscope (HITACHI Co., Kyoto, Japan). The prepared capsule solution was powdered via freeze-drying and stored frozen. To measure the amount of extract contained in the capsules, 10 mg capsules were dispersed in a mixed solution of distilled water and ethanol (1:1 v / v), ground using an ultrasonicator (VCX500, Sonics and Materials Inc., Newtown, CT, USA), and centrifuged. The supernatant was collected and quantitatively analyzed by measuring the absorbance at 420 nm using a UV / VIS spectrophotometer (UV-160A, Shimadzu Co., Kyoto, Japan) with a flavonoid assay method. Capsules containing extracts of vitamin leaf (He) and sappanwood (Be), prepared using gamma-cyclodextrin, were confirmed to be spherical in shape and have a size of 300–400 nm when observed under a scanning electron microscope. The loading efficiency of the extracts within the capsules was 90%. Gamma-cyclodextrin has a capsule shape in which the hydroxyl groups are chemically oriented outward, making the exterior hydrophilic, while the CH groups and ether bonds are oriented inward, making the interior hydrophobic. Therefore, gamma-cyclodextrin is utilized in many fields, including cosmetics, food, pharmaceuticals, and pesticides, due to its characteristic ability to encapsulate hydrophobic bioactive substances. Flavonoid and polyphenol components of extracts that are highly hydrophobic and soluble in organic solvents can be easily encapsulated within gamma-cyclodextrin.

[0107] Figure 8 shows the antioxidant activity results of Preparation Example 3. Referring to Figure 8, at 0.1 mg / ml of each extract, antioxidant inhibitory activity was observed as 80±1.42% for Be, 90±3.24% for He, and 100±1.2% for the Be and He mixed extract. Mixing the extracts showed an effect of increasing antioxidant activity.

[0108] Figure 9 shows the SEM and particle distribution results of Preparation Example 3. Referring to Figure 9, SEM analysis confirmed a particle size of 300-400 nm, and the particle distribution measurement showed a size pattern of the same.

[0110] Preparation Example 4 - Film Preparation

[0111] Films were prepared with a fixed total concentration of 2 wt%, using compositional ratios of water-soluble chitosan (GC) and gellan gum (GEL) of GC / GEL (0.7:0.3, 0.5:0.5, 0.3:0.7). Films loaded with drugs effective against dental caries and periodontal disease were prepared using the same composition. GC was dissolved in d-PBS and GEL in triple-distilled water, respectively, at 90°C and 200 rpm until clear and transparent solutions were obtained. 25 ml aliquots were cast into PET dishes and semi-dried in a 65°C heat dryer for 6 hours. The semi-dried films were freeze-dried before use.

[0112] The morphology of the film according to each composition was shown in images of the dry state and the wet state. Gellan gum (GEL), used as a thickening agent, was found to have adhesive properties as it became wet with water (OH).

[0114] Evaluation Example 4 - Tensile Strength Measurement

[0115] The tensile strength of the film prepared in Preparation Example 4 was measured using the American Society of Testing and Materials (ASTM) standard method D882-01 (20). All film samples used for measurement were prepared in a size of 50 mm x 8 mm and stored for 48 hours at 23±2℃ and 52±2% relative humidity prior to measurement. Each tensile property was calculated using a tensile property tester (Withlab Co., Ltd., Anyang, Korea).

[0116] Figure 11 shows the tensile strength measurement results of Preparation Example 4. Referring to Figure 11, the initial distance between grips was 50 mm, and the cross head speed was 30 mm / min. It was confirmed that the tensile strength decreased as the content of GC (chitosan) increased, and the tensile strength increased as the content of GEL (gellan gum) increased. Values ​​of 7.5 1.2 MPa were calculated for GC:GEL (0.7:0.3), 9.5 2.1 MPa for GC:GEL (OS:0.5), and 20.4 2.4 MPa for GC:GEL (0.3:0.7).

[0118] Example 1 - Preparation of extract-loading film

[0119] Chitosan (GC) and gellan gum (GEL) were mixed to produce films containing extract-carrying capsules in a GC / GEL (0.5:0.5) composition ratio. Capsule-carrying films were prepared by loading 2% by weight of each He extract-carrying capsule, 2% by weight of Be extract-carrying capsule, and 2% by weight of a mixture of He and Be capsules onto the film in a GC / GEL (0.5:0.5) composition ratio.

[0120] Figure 12 is a photograph of the film of Example 1. Referring to Figure 12, it was confirmed that the film was maintained without change in shape and the extract was released slowly after being treated in a 37°C incubator for 60 minutes.

[0122] Evaluation Example 5 - Measurement of Swelling Degree

[0123] The degree of swelling was the specimen prepared in Example 1 (30 x 30 mm 2 The sample was prepared to weigh 0.3g, immersed in 20 ml of PBS at 37°C, and evaluated by measuring its weight within 24 hours. The value was calculated by dividing the weight of the solid component swollen in water by the initial solid component weight and multiplying the result by 100. Figure 13 shows the swelling degree results according to the GC / GEL composition. Referring to Figure 13, it was observed that the initial swelling degree value increased as the proportion of GC increased, and the swelling degree value rose after 48 hours as the proportion of GEL increased. The same pattern was observed in the film loaded with the extract. It was confirmed that the moisture content was maintained while sustained release of the extract occurred.

[0125] Comparative Example 1

[0126] A capsule-containing film was prepared by using chitosan (GC) and gellan gum (GEL) in a GC / GEL (0.1:0.9) composition ratio to make the film 2% by weight of capsules loaded with a mixture of He and Be.

[0128] Comparative Example 2

[0129] A capsule-containing film was prepared by using chitosan (GC) and gellan gum (GEL) in a GC / GEL (0.9:0.1) composition ratio to make the film 2% by weight of capsules loaded with a mixture of He and Be.

[0131] Figure 14 shows the film results according to the GC / GEL composition. Referring to Figure 14, it was observed that as the concentration of GEL increased, the film thickness became thinner and tore easily. Additionally, it was observed that as the concentration of GC increased, the thickness became thicker and exhibited an uneven shape.

[0133] Comparative Example 3

[0134] A film was prepared by adding chitosan (GC) and gellan gum (GEL) in a GC / GEL (0.5:0.5) composition ratio to the film, such that the mixture of the He and Be extracts themselves was 2% by weight.

[0136] Figure 15 is a film photograph of Comparative Example 3. Referring to Figure 15, it was confirmed that cracking occurred when the extract concentration was 1 wt% or more in the case of non-encapsulation.

[0138] Comparative Example 4

[0139] A capsule-containing film was prepared by using chitosan (GC) and gellan gum (GEL) in a GC / GEL (0.5:0.5) composition ratio to make the film 3.5% by weight of capsules loaded with a mixture of He and Be.

[0141] Figure 16 is a photograph of the film of Comparative Example 4. Referring to Figure 16, it was confirmed that when a film loaded with more than 3% by weight of Sappanwood and Vitamin Leaf extract was prepared, the thickness of the film increased and it did not possess swelling properties.

[0143] As described above, although the present invention has been explained with reference to preferred test examples, those skilled in the art will understand that various modifications and changes can be made to the present invention without departing from the spirit and scope of the invention as set forth in the following claims.

Claims

Claim 1 A wound healing film comprising a gellan gum and a chitosan layer containing capsules loaded with sappanwood extract and vitamin leaf extract, wherein the capsules are sappanwood extract and vitamin leaf extract-cyclodextrin inclusion complexes, the weight ratio of the gellan gum to the chitosan is 7:3 to 3:7, and the total content of the sappanwood extract and vitamin leaf extract in the film is 1% by weight or more. Claim 2 A wound healing film according to claim 1, wherein the sappanwood extract and vitamin leaf extract are alcohol extracts. Claim 3 delete Claim 4 A method for manufacturing a wound healing film comprising the steps of heat-drying and freeze-drying a coated capsule containing sappanwood extract and vitamin leaf extract, gellan gum, and a chitosan solution, wherein the capsule is a sappanwood extract and vitamin leaf extract-cyclodextrin inclusion complex, the concentrations of gellan gum and chitosan in the solution are each 0.5 to 3 weight%, the weight ratio is 7:3 to 3:7, and the concentration of the capsule is 1 to 3 weight% relative to the total weight of the solution. Claim 5 A method for manufacturing a wound healing film according to claim 4, further comprising the step of mixing a capsule solution with a gellan gum and a chitosan solution to produce a capsule containing the sappanwood extract and vitamin leaf extract, and a gellan gum and chitosan solution. Claim 6 A method for manufacturing a wound healing film according to claim 5, further comprising the step of mixing a gellan gum solution and a chitosan solution to prepare the gellan gum and chitosan solution, wherein the solvent of the chitosan solution is a cation-containing solvent. Claim 7 A method for manufacturing a wound healing film according to claim 6, wherein the temperature of the mixed gellan gum solution and chitosan solution is 60 to 90 ℃. Claim 8 delete Claim 9 delete Claim 10 delete

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