A herbal medicine composition for memory improvement and a health functional food composition comprising the same

Abstract

The present invention relates to a herbal medicine composition for improving memory and Dr. Park's MEMORY PREMIUM containing the same. The herbal medicine composition according to the present invention comprises red ginseng concentrate, purified water, a mixed concentrate of Poria cocos sclerotium (other processed agricultural products), fructooligosaccharide, pear concentrate, royal jelly, deer antler extract, and a complex Scutellaria root extract (other processed products). The herbal medicine composition of the present invention exhibits excellent effects, such as significantly improving behavioral indicators in an animal model with induced memory impairment, protecting the cholinergic nervous system by inhibiting acetylcholinesterase (AChE) activity in the hippocampus, and increasing the expression of brain-derived neurotrophic factor (BDNF) and its receptor TrkB. Accordingly, the herbal medicine composition of the present invention can be very usefully utilized as a health functional food for improving memory and cognitive ability.

Description

Technology Field

[0001] The present invention relates to a herbal medicine composition for improving memory and a health functional food composition containing the same. Specifically, the present invention relates to a herbal medicine composition and a health functional food composition containing the same, which includes red ginseng concentrate, purified water, a mixed concentrate of Poria cocos sclerotium (other processed agricultural products), fructooligosaccharide, pear concentrate, sweetened honey, deer antler extract, and complex Scutellaria root extract (other processed products), and which can improve memory and cognitive function by protecting the cholinergic nervous system and increasing the expression of hippocampal neurotrophic factors. Background Technology

[0003] Recently, due to the accelerated entry into an aging society and exposure to chronic stress, the population complaining of memory loss and cognitive decline is continuously increasing, leading to a growing demand for memory-improving materials derived from natural products with minimal risk of side effects. In particular, various herbal medicines such as red ginseng, Cornus fruit, Polygala root, and Acorus calamus rhizome have traditionally been known to be effective in improving memory and cognitive function; however, when provided in the form of a single herbal medicine or a simple mixed extract, there are limitations in that the protective effect on the cholinergic nervous system and the effect of enhancing neurotrophic factor expression are insufficient.

[0004] In addition, even in the case of conventional compositions that mix multiple herbal ingredients, the critical significance according to the content and mixing ratio of each component has not been clearly identified, so there is a problem in that it is difficult to ensure the reproducibility and consistency of memory improvement effects by simply mixing various types of herbal ingredients. Therefore, there is a need to develop a herbal composition that can significantly enhance the effects of protecting the cholinergic nervous system and promoting the expression of neurotrophic factors by optimizing the types, content, and mutual mixing ratios of multiple herbal ingredients and extract components. Prior art literature

[0006] Republic of Korea Registered Patent KR 10-1682637 B1 The problem to be solved

[0007] The present invention aims to overcome the limitations of conventional memory-improving materials provided in the form of a single herbal medicine or a simple mixed extract, namely, the lack of reproducibility and consistency in the effects of protecting the cholinergic nervous system and enhancing the expression of neurotrophic factors because the critical significance according to the content and mixing ratio of each component has not been identified.

[0008] Specifically, the present invention aims to provide a herbal medicine composition capable of significantly enhancing the protective effect on the cholinergic nervous system and the expression of hippocampal neurotrophic factors by combining red ginseng concentrate, purified water, mixed concentrate, fructooligosaccharide, pear concentrate, sweet honey, deer antler extract, and complex goldenrod extract in a specific content range, and further limiting each herbal medicine and component constituting the mixed concentrate and complex goldenrod extract to a specific narrow range of mixing ratios.

[0009] Furthermore, the present invention aims to provide a health functional food composition for improving memory or cognitive ability comprising the above-mentioned herbal medicine composition as an active ingredient.

[0010] However, the problems that this invention seeks to solve are not limited to those mentioned above, and other unmentioned problems will be clearly understood by a person skilled in the art from the description below. means of solving the problem

[0012] To achieve the above objectives, one aspect of the present invention is a herbal medicine composition for improving memory comprising red ginseng concentrate, purified water, mixed concentrate, fructooligosaccharide, pear concentrate, royal jelly, deer antler extract, and complex goldenrod extract as active ingredients, wherein

[0013] The above herbal medicine composition is mixed with 2 to 8 parts by weight of red ginseng concentrate, 35 to 45 parts by weight of purified water, 15 to 25 parts by weight of mixed concentrate, 15 to 20 parts by weight of fructooligosaccharide, 8 to 12 parts by weight of pear concentrate, 2 to 8 parts by weight of sugarcane honey, 1 to 3 parts by weight of deer antler extract, and 0.1 to 1 part by weight of complex Scutellaria root extract.

[0014] The above red ginseng concentrate and deer antler extract are included in a weight ratio of 1:0.2 to 0.6, and

[0015] The above mixed concentrate consists of Poria cocos, Acorus calamus rhizome, Polygala tenuifolia root, Longan false seed husk, Poria cocos, Rehmannia glutinosa, Cuscuta japonica seeds, Cornus fruit, Rubus fructus, Turmeric extract, Gastrodia elata root, Paeonia lactiflora root, Cinnamon, Astragalus membranaceus, Cnidium officinale rhizome, Panax ginseng, Eucommia ulmoides leaves, Schisandra chinensis fruit, Lycium barbarum fruit, Dioscorea japonica root, Ophiopogon japonicus root, Rehmannia glutinosa raw, Asparagus cochinchinensis tuberous root, Glycyrrhiza glabra, Angelica gigas root, Ginger, Jujube, and large gourd tuberous root.

[0016] The above complex golden extract is a herbal medicine composition for improving memory, consisting of golden extract, licorice extract, jujube extract, astragalus extract, dextrin, and lactic acid.

[0018] Another aspect of the present invention is a health functional food composition for improving memory, comprising the above herbal medicine composition.

[0020] Another aspect of the present invention comprises the step of (a) extracting and concentrating Poria cocos, Acorus calamus rhizome, Polygala tenuifolia root, Longan false seed husk, Poria cocos, Rehmannia glutinosa, Cuscuta japonica seeds, Cornus officinalis fruit, Rubus fructus, Turmeric extract, Gastrodia elata root, Paeonia lactiflora root, Cinnamon, Astragalus membranaceus, Cnidium officinale rhizome, Panax ginseng, Eucommia ulmoides leaves, Schisandra chinensis fruit, Lycium barbarum fruit, Dioscorea root, Ophiopogon japonicus root, Rehmannia glutinosa raw, Asparagus cochinchinensis tuberous root, Glycyrrhiza glabra, Angelica gigas root, Ginger, Jujube, and Cynanchum wilfordii tuberous root to obtain a mixed concentrate;

[0021] (b) a step of obtaining a complex golden extract by mixing dextrin and lactic acid with golden extract, licorice extract, jujube extract and astragalus extract; and

[0022] (c) a step of mixing red ginseng concentrate, purified water, fructooligosaccharide, pear concentrate, royal jelly, and deer antler extract with the mixed concentrate of step (a) and the complex golden extract of step (b);

[0023] Includes,

[0024] The above step (c) is a method for preparing a herbal medicine composition for improving memory, wherein the above steps are mixed with 2 to 8 parts by weight of red ginseng concentrate, 35 to 45 parts by weight of purified water, 15 to 25 parts by weight of mixed concentrate, 15 to 20 parts by weight of fructooligosaccharide, 8 to 12 parts by weight of pear concentrate, 2 to 8 parts by weight of sugarcane honey, 1 to 3 parts by weight of deer antler extract, and 0.1 to 1 part by weight of complex goldenrod extract. Effects of the invention

[0026] The present invention can significantly improve behavioral indicators such as passive avoidance tests and Y-maze tests in animal models in which memory impairment is induced by scopolamine by combining red ginseng concentrate, purified water, mixed concentrate, fructooligosaccharide, pear concentrate, sweet honey, deer antler extract, and complex goldenrod extract within a specific content range.

[0027] In addition, the Poria cocos, Acorus calamus rhizome, Polygala tenuifolia root, and Longan husk in the mixed concentrate, and the Scutellaria baicalensis concentrate in the complex Scutellaria baicalensis extract are organically combined to effectively inhibit acetylcholinesterase (AChE) activity in the hippocampus and maintain acetylcholine (ACh) content, thereby protecting the cholinergic nervous system.

[0028] Furthermore, by limiting the mixing ratio of each herbal medicine and component constituting the mixed concentrate and complex golden extract to a specific narrow range, the expression of brain-derived neurotrophic factor (BDNF) and its receptor TrkB in the hippocampus can be significantly increased compared to cases where multiple types of herbal medicines are simply mixed, thereby improving synaptic plasticity.

[0029] Consequently, since the present invention utilizes components derived from natural herbal medicines to exhibit excellent and reproducible effects in improving memory and cognitive function, it can be very usefully utilized as a material for health functional foods and functional foods. Specific details for implementing the invention

[0031] The present invention will be described in detail below with reference to embodiments thereof. However, the following embodiments are presented as examples of the present invention, and if it is determined that a detailed description of a technology or configuration well known to those skilled in the art may unnecessarily obscure the essence of the present invention, such detailed description may be omitted, and the present invention is not limited by this. The present invention is capable of various modifications and applications within the scope of the claims set forth below and the equivalent scope interpreted therefrom.

[0032] Additionally, the terminology used in this specification is used to appropriately describe preferred embodiments of the present invention, and may vary depending on the intent of the user or operator or the conventions of the field to which the present invention belongs.

[0033] Therefore, the definitions of these terms should be based on the content throughout this specification. Throughout the specification, when a part is described as "comprising" a certain component, this means that, unless specifically stated otherwise, it does not exclude other components but may include additional components.

[0034] Throughout this specification, '%' used to indicate the concentration of a particular substance is (w / w) % for solid / solid, (w / v) % for solid / liquid, and (v / v) % for liquid / liquid, unless otherwise noted.

[0036] One aspect of the present invention is a herbal medicine composition for improving memory comprising red ginseng concentrate, purified water, mixed concentrate, fructooligosaccharide, pear concentrate, royal jelly, deer antler extract, and complex goldenrod extract as active ingredients,

[0037] The above herbal medicine composition is mixed with 2 to 8 parts by weight of red ginseng concentrate, 35 to 45 parts by weight of purified water, 15 to 25 parts by weight of mixed concentrate, 15 to 20 parts by weight of fructooligosaccharide, 8 to 12 parts by weight of pear concentrate, 2 to 8 parts by weight of sugarcane honey, 1 to 3 parts by weight of deer antler extract, and 0.1 to 1 part by weight of complex Scutellaria root extract.

[0038] The above red ginseng concentrate and deer antler extract are included in a weight ratio of 1:0.2 to 0.6, and

[0039] The above mixed concentrate consists of Poria cocos, Acorus calamus rhizome, Polygala tenuifolia root, Longan false seed husk, Poria cocos, Rehmannia glutinosa, Cuscuta japonica seeds, Cornus fruit, Rubus fructus, Turmeric extract, Gastrodia elata root, Paeonia lactiflora root, Cinnamon, Astragalus membranaceus, Cnidium officinale rhizome, Panax ginseng, Eucommia ulmoides leaves, Schisandra chinensis fruit, Lycium barbarum fruit, Dioscorea japonica root, Ophiopogon japonicus root, Rehmannia glutinosa raw, Asparagus cochinchinensis tuberous root, Glycyrrhiza glabra, Angelica gigas root, Ginger, Jujube, and large gourd tuberous root.

[0040] The above complex golden extract is a herbal medicine composition for improving memory, consisting of golden extract, licorice extract, jujube extract, astragalus extract, dextrin, and lactic acid.

[0041] The term "herbal medicine composition" in this specification may refer to a composition comprising raw materials derived from animals and plants that have been traditionally used for medicinal purposes, such as ginseng, deer antler, and scutellaria, and their extracts as active ingredients, and the formulation is not particularly limited, such as liquid, powder, or solid form.

[0042] The term "mixed concentrate" in this specification may be used interchangeably with "mixed concentrate of Poria cocos sclerotia, etc. (other processed agricultural products)," and includes Poria cocos sclerotia (Poria cocos), Acorus calamus rhizome (Acorus calamus), Polygala tenuifolia root (Poria cocos), Longan fruit (Longan flesh), Poria cocos sclerotium (Poria cocos), Rehmannia glutinosa (Sukjihwang), Cuscuta japonica seeds (Cuscuta japonica), Cornus officinalis fruit (Cuscuta japonica), Rubus fructus, Turmeric extract (Turmeric extract), Gastrodia elata root (Gastrodia elata), Paeonia lactiflora root (Paeonia lactiflora), Cinnamon, Astragalus membranaceus, Cnidium officinale rhizome (Cnidium officinale), Ginseng, Eucommia ulmoides leaves (Eucommia ulmoides), Schisandra chinensis fruit (Schisandra chinensis), Lycium chinense fruit (Lycium chinense), Dioscorea japonica root (Dioscorea japonica), Ophiopogon japonicus root (Ophiopogon japonicus), Rehmannia glutinosa (Fresh Rehmannia glutinosa), Asparagus cochinchinensis tuber (Asparagus cochinchinensis), Glycyrrhiza glabra root (Angelica gigas), It may refer to a mixed extract concentrate obtained by processing medicinal herbs such as ginger, jujube, and large gourd root (Baeksuo) using conventional extraction and concentration methods.

[0043] The term "complex golden extract" in this specification may be used interchangeably with "complex golden extract (other processed products)" and may refer to a complex extract concentrate prepared by adding dextrin and lactic acid to golden extract, licorice extract, jujube extract and astragalus extract.

[0044] The extract or extract according to the present invention may be obtained by extracting and separating from various medicinal herbs using extraction and separation methods known in the art, and the "extract," "extract," or "concentrate" defined in the present invention may be obtained by using a suitable solvent as a medicinal herb, including red ginseng, Poria cocos sclerotium (Poria cocos), Acorus calamus rhizome (Acorus calamus), Polygala tenuifolia root (Poria cocos), Longan fruit (Longan fruit), Poria cocos sclerotium (Poria cocos), Rehmannia glutinosa (Sukjihwang), Cuscuta japonica seeds (Cuscuta japonica), Cornus officinalis fruit (Cuscuta japonica), Rubus fructus, Turmeric, Gastrodia elata root (Gastrodia elata), Paeonia lactiflora root (Paeonia lactiflora), Cinnamon, Astragalus membranaceus, Cnidium officinale rhizome (Cnidium officinale), Ginseng, Eucommia ulmoides leaves (Euchong), Schisandra chinensis fruit (Schisandra chinensis), Lycium chinense fruit (Lycium chinense), Dioscorea japonica root (Dioscorea japonica), Ophiopogon japonicus root (Ophiopogon japonicus), Rehmannia glutinosa (Saengjihwang). It is an extract or concentrate thereof derived from one or more of the following: Asparagus cochinchinensis tuberous root (Asparagus cochinchinensis), licorice, Angelica gigas root (Angelica sinensis), ginger, jujube, Cynanchum wilfordii tuberous root (Polygonum multiflorum), pear, deer antler, Scutellaria baicalensis, and licorice; for example, it includes all of the crude extract, polar solvent-soluble extract, or non-polar solvent-soluble extract. As a suitable solvent for preparing an extract using the above herbal medicine, any organic solvent permitted in food science or pharmaceutical science may be used, and water or organic solvents may be used, but are not limited thereto, various solvents such as purified water, alcohols having 1 to 4 carbon atoms including methanol, ethanol, propanol, isopropanol, butanol, acetone, ether, benzene, chloroform, ethyl acetate, methylene chloride, hexane, and cyclohexane may be used alone or in combination.As an extraction method, any one of the following may be selected and used: water bath extraction, hot water extraction, cold maceration extraction, reflux extraction, solvent extraction, steam distillation, ultrasonic extraction, elution, or pressing. Additionally, the desired extract may undergo a conventional fractionation process or be purified using a conventional purification method.

[0045] There are no limitations on the method for preparing the extract of the present invention, and any known method may be used. For example, the extract included in the composition of the present invention may be prepared in a powder state by additional processes such as spray drying, vacuum distillation, or freeze-drying, using a primary extract obtained by the aforementioned hot water extraction or solvent extraction method. Additionally, a further purified fraction may be obtained from the primary extract using various chromatographic methods such as silica gel column chromatography, thin layer chromatography, or high performance liquid chromatography. Accordingly, in the present invention, the term "extract" encompasses all extracts, fractions, and purified products obtained at each stage of extraction, fractionation, or purification, as well as their dilutions, concentrates, or dried products.

[0046] The term "memory improvement" in this specification may mean maintaining or improving normal learning and memory abilities, or restoring memory ability that has been reduced by aging, stress, or damage to the cholinergic nervous system to a normal level. Specifically, in the present invention, the memory improvement may include the action of protecting the cholinergic nervous system by inhibiting acetylcholinesterase (AChE) activity in the hippocampus and maintaining acetylcholine (ACh) content, and the action of improving synaptic plasticity by increasing the expression of brain-derived neurotrophic factor (BDNF) and its receptor TrkB.

[0047] In the present invention, the red ginseng concentrate may be included in an amount of 2 to 8 parts by weight, 2 to 6 parts by weight, 3 to 8 parts by weight, or 3 to 6 parts by weight, and specifically, may be included in an amount of 5 parts by weight, but is not limited thereto. According to one embodiment of the present invention, it was confirmed that behavioral indicators related to memory improvement are significantly superior when the content of the red ginseng concentrate is included within the numerical range, compared to when the content is below the lower limit or exceeds the upper limit of the numerical range.

[0048] In the present invention, the purified water may be included in an amount of 35 to 45 parts by weight, 35 to 40 parts by weight, or 38 to 42 parts by weight, and specifically in an amount of 39.4 parts by weight, but is not limited thereto.

[0049] In the present invention, the mixed concentrate may be included in an amount of 15 to 25 parts by weight, 15 to 20 parts by weight, or 18 to 22 parts by weight, and specifically, may be included in an amount of 20 parts by weight, but is not limited thereto. According to one embodiment of the present invention, it was confirmed that when the content of the mixed concentrate falls outside the above numerical range and when the mixed concentrate itself is not included, the protective effect on the cholinergic nervous system and the memory improvement effect are significantly superior when included within the above numerical range.

[0050] In the present invention, the fructooligosaccharide may be included in an amount of 15 to 20 parts by weight, specifically 18.3 parts by weight, but is not limited thereto.

[0051] In the present invention, the concentrated extract may be included in an amount of 8 to 12 parts by weight, specifically 10 parts by weight, but is not limited thereto.

[0052] In the present invention, the above-mentioned honey may be included in an amount of 2 to 8 parts by weight, specifically 5 parts by weight, but is not limited thereto.

[0053] In the present invention, the deer antler extract may be included in an amount of 1 to 3 parts by weight, specifically 2 parts by weight, but is not limited thereto.

[0054] In the present invention, the complex golden extract may be included in an amount of 0.1 to 1 part by weight, 0.1 to 0.5 parts by weight, or 0.2 to 0.5 parts by weight, and specifically, may be included in an amount of 0.3 parts by weight, but is not limited thereto. According to one embodiment of the present invention, it was confirmed that the protective effect on the cholinergic nervous system is significantly superior when the content of the complex golden extract is included within the above numerical range, compared to when the content falls outside the above numerical range and when the complex golden extract itself is not included.

[0055] In the present invention, the red ginseng concentrate and the deer antler extract may be included in a weight ratio of 1:0.2 to 0.6, specifically in a weight ratio of 1:0.4, but are not limited thereto. According to one embodiment of the present invention, it was confirmed that even if the red ginseng concentrate and the deer antler extract are within the same individual content range, when the weight ratio deviates from the above (e.g., 1:1.0 or 1:0.17), behavioral indicators related to memory improvement and the protective effect on the cholinergic nervous system are significantly reduced. Through this, it was confirmed that the above weight ratio has critical significance that is expressed only at a specific ratio, rather than being a simple sum of individual contents.

[0056] In the present invention, the mixed concentrate comprises 7 to 8 parts by weight of Poria cocos, 7 to 8 parts by weight of Acorus calamus rhizome, 7 to 8 parts by weight of Polygala tenuifolia root, 7 to 8 parts by weight of Longan false seed husk, 2 to 3 parts by weight of Poria cocos, 2 to 3 parts by weight of Rehmannia glutinosa, 5 to 6 parts by weight of Cuscuta japonica seeds, 2 to 3 parts by weight of Cornus officinalis fruit, 1.5 to 2 parts by weight of Rubus fructus, 7 to 8 parts by weight of Turmeric extract, 2 to 3 parts by weight of Gastrodia elata root, 2 to 3 parts by weight of Paeonia lactiflora root, 2 to 3 parts by weight of Cinnamon, 2 to 3 parts by weight of Astragalus membranaceus, 2 to 3 parts by weight of Cnidium officinale rhizome, 2 to 3 parts by weight of Panax ginseng, 2 to 3 parts by weight of Eucommia ulmoides leaves, 2 to 3 parts by weight of Schisandra chinensis fruit, 2 to 3 parts by weight of Lycium chinense fruit, and Dioscorea japonica root. It may consist of 2 to 3 parts by weight of Ophiopogon japonicus root, 2 to 3 parts by weight of Rehmannia glutinosa, 2 to 3 parts by weight of Asparagus cochinchinensis tuberous root, 2 to 3 parts by weight of Glycyrrhiza glabra, 2 to 3 parts by weight of Angelica gigas root, 3 to 4 parts by weight of ginger, 3 to 4 parts by weight of jujube, and 1 to 1.5 parts by weight of Prunella vulgaris tuberous root.

[0057] Specifically, the above mixed concentrate comprises 7.74 parts by weight of Poria cocos, 7.74 parts by weight of Acorus calamus rhizome, 7.74 parts by weight of Polygala tenuifolia root, 7.74 parts by weight of Longan false seed husk, 2.58 parts by weight of Poria cocos, 2.58 parts by weight of Rehmannia glutinosa, 5.16 parts by weight of Cuscuta japonica seeds, 2.58 parts by weight of Cornus officinalis fruit, 1.94 parts by weight of Rubus fructus, 7.74 parts by weight of Turmeric extract, 2.58 parts by weight of Gastrodia elata root, 2.58 parts by weight of Paeonia lactiflora root, 2.58 parts by weight of Cinnamon, 2.58 parts by weight of Astragalus membranaceus, 2.58 parts by weight of Cnidium officinale rhizome, 2.58 parts by weight of Panax ginseng, 2.58 parts by weight of Eucommia ulmoides leaves, 2.58 parts by weight of Schisandra chinensis fruit, 2.58 parts by weight of Lycium chinense fruit, and 2.58 parts by weight of Dioscorea japonica root. It may consist of parts by weight, 2.58 parts by weight of Ophiopogon japonicus root, 2.58 parts by weight of Rehmannia glutinosa, 2.58 parts by weight of Asparagus cochinchinensis tuberous root, 2.58 parts by weight of Glycyrrhiza glabra, 2.58 parts by weight of Angelica gigas root, 3.23 parts by weight of ginger, 3.23 parts by weight of jujube, and 1.3 parts by weight of Prunella vulgaris tuberous root.

[0058] Or, a mixed concentrate of Poria cocos sclerotia, etc. (other processed agricultural products) comprises 7.74 parts by weight of Poria cocos sclerotia (Fúxīn), 7.74 parts by weight of Acorus calamus rhizome (Acorus calamus), 7.74 parts by weight of Polygala tenuifolia root (Poria cocos), 7.74 parts by weight of Longan fruit husk (Longan flesh), 2.58 parts by weight of Poria cocos sclerotia (Poria cocos), 2.58 parts by weight of Rehmannia glutinosa (Shu Dihuang), 5.16 parts by weight of Cuscuta japonica seeds (Cuscuta japonica), 2.58 parts by weight of Cornus officinalis fruit (Cuscuta japonica), 1.94 parts by weight of Rubus coreanus, 7.74 parts by weight of turmeric extract (turmeric extract), 2.58 parts by weight of Gastrodia elata root (Gastrodia elata), 2.58 parts by weight of Paeonia lactiflora root (Paeonia lactiflora), 2.58 parts by weight of cinnamon, and 2.58 parts by weight of Astragalus membranaceus. It may consist of 2.58 parts by weight of Cnidium rhizome (Cnidium), 2.58 parts by weight of ginseng, 2.58 parts by weight of Eucommia leaves (Eucommia), 2.58 parts by weight of Schisandra fruit (Schisandra), 2.58 parts by weight of Lycium chinense fruit (Lycium chinense), 2.58 parts by weight of yam root (Yam), 2.58 parts by weight of Ophiopogon japonicus root (Ophiopogon japonicus), 2.58 parts by weight of Rehmannia glutinosa (Rehmannia glutinosa), 2.58 parts by weight of Asparagus cochinchinensis tuberous root (Asparagus cochinchinensis), 2.58 parts by weight of licorice, 2.58 parts by weight of Angelica gigas root (Angelica gigas), 3.23 parts by weight of ginger, 3.23 parts by weight of jujube, and 1.3 parts by weight of Cynanchum wilfordii tuberous root (Polygonum multiflorum).

[0059] According to one embodiment of the present invention, it was confirmed that when the four types are included, the inhibitory effect on AChE activity in the hippocampus and the effect on increasing BDNF and TrkB expression are significantly superior compared to when the four types are excluded from the mixed concentrate: Poria cocos, Acorus calamus rhizome, Polygala tenuifolia root, and Longan husk.

[0060] In the present invention, the complex golden extract may comprise 35 to 40 parts by weight of golden extract, 25 to 30 parts by weight of licorice extract, 7 to 10 parts by weight of jujube extract, 6 to 8 parts by weight of Astragalus extract, 8 to 12 parts by weight of dextrin, and 8 to 12 parts by weight of lactic acid.

[0061] Specifically, the composite Scutellaria baicalensis extract may consist of 36.8 parts by weight of Scutellaria baicalensis concentrate, 27.2 parts by weight of Glycyrrhiza uralensis concentrate, 8.8 parts by weight of jujube concentrate, 7.2 parts by weight of Astragalus membranaceus concentrate, 10 parts by weight of dextrin, and 10 parts by weight of lactic acid.

[0062] Alternatively, the composite Scutellaria baicalensis extract (other processed product) may consist of 36.8 parts by weight of Scutellaria baicalensis concentrate, 27.2 parts by weight of Glycyrrhiza uralensis concentrate, 8.8 parts by weight of jujube concentrate, 7.2 parts by weight of Astragalus membranaceus concentrate, 10 parts by weight of dextrin, and 10 parts by weight of lactic acid.

[0063] According to one embodiment of the present invention, it was confirmed that the case including the Scutellaria baicalensis concentrate exhibited significantly superior inhibitory effect on AChE activity in the hippocampus and increasing effect on BDNF and TrkB expression, as compared to the case excluding the Scutellaria baicalensis concentrate from the composite Scutellaria baicalensis extract.

[0064] In the present invention, the herbal composition may be in the form of a decoction, pill, tea, pellet, or paste, but is not limited thereto.

[0065] As used herein, the term "decoction" may refer to a form in which herbal medicinal materials are added to water and boiled to drink the resulting liquid, "pill" may refer to a product formed by kneading herbal medicinal material powder into small pellet shapes, "tea" may refer to a form in which herbal medicinal materials are steeped in water for drinking, "dan" may refer to a refined pill form made from herbal medicinal materials, and "paste" may refer to a thick concentrated form prepared by simmering herbal medicinal materials for a long period of time.

[0066] In the present invention, the herbal composition may increase the expression of brain-derived neurotrophic factor (BDNF) in an animal model with induced memory impairment, compared to a control group not treated with the herbal composition.

[0068] Another aspect of the present invention is a health functional food composition for improving memory, comprising the herbal composition.

[0069] Specifically, it is a health functional food composition for improving memory that includes the above-mentioned herbal medicine composition as an active ingredient.

[0070] In addition to including the herbal medicine composition as an active ingredient, the health functional food composition of the present invention may include various flavoring agents or natural carbohydrates, etc., as additional ingredients, as in conventional health functional food compositions.

[0071] In addition, the above health functional food composition may include, in addition to the herbal medicine composition which is an active ingredient, various nutritional supplements, vitamins, minerals (electrolytes), flavoring agents such as synthetic flavoring agents and natural flavoring agents, coloring agents and fillers (cheese, chocolate, etc.), pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonating agents used in carbonated beverages, etc.

[0072] The health functional food composition of the present invention may be manufactured and processed in the form of tablets, capsules, powders, granules, liquids, pills, etc. In the present invention, the term "health functional food composition" refers to a food manufactured and processed using raw materials or ingredients having functional properties useful to the human body pursuant to Article 6727 of the Health Functional Foods Act, and means consuming it for the purpose of obtaining useful effects for health purposes, such as regulating nutrients or physiological actions on the structure and function of the human body.

[0074] Another aspect of the present invention comprises the step of (a) extracting and concentrating Poria cocos, Acorus calamus rhizome, Polygala tenuifolia root, Longan false seed husk, Poria cocos, Rehmannia glutinosa, Cuscuta japonica seeds, Cornus officinalis fruit, Rubus fructus, Turmeric extract, Gastrodia elata root, Paeonia lactiflora root, Cinnamon, Astragalus membranaceus, Cnidium officinale rhizome, Panax ginseng, Eucommia ulmoides leaves, Schisandra chinensis fruit, Lycium barbarum fruit, Dioscorea root, Ophiopogon japonicus root, Rehmannia glutinosa raw, Asparagus cochinchinensis tuberous root, Glycyrrhiza glabra, Angelica gigas root, Ginger, Jujube, and Cynanchum wilfordii tuberous root to obtain a mixed concentrate;

[0075] (b) a step of obtaining a complex golden extract by mixing dextrin and lactic acid with golden extract, licorice extract, jujube extract and astragalus extract; and

[0076] (c) a step of mixing red ginseng concentrate, purified water, fructooligosaccharide, pear concentrate, royal jelly, and deer antler extract with the mixed concentrate of step (a) and the complex golden extract of step (b);

[0077] Includes,

[0078] The above step (c) is a method for preparing a herbal medicine composition for improving memory, wherein the above steps are mixed with 2 to 8 parts by weight of red ginseng concentrate, 35 to 45 parts by weight of purified water, 15 to 25 parts by weight of mixed concentrate, 15 to 20 parts by weight of fructooligosaccharide, 8 to 12 parts by weight of pear concentrate, 2 to 8 parts by weight of sugarcane honey, 1 to 3 parts by weight of deer antler extract, and 0.1 to 1 part by weight of complex goldenrod extract.

[0080] Another aspect of the present invention is the use of the above herbal medicine composition for improving memory.

[0082] Hereinafter, the structure of the present invention and the resulting effects are to be explained in more detail through specific embodiments and comparative examples. However, these embodiments are intended to explain the present invention more specifically, and the scope of the present invention is not limited to these embodiments.

[0084] Preparation of herbal medicine compositions of examples and comparative examples

[0085] In order to confirm the critical significance of the mixing ratio and weight ratio of the herbal medicine composition according to the present invention, herbal medicine compositions of the examples and comparative examples were prepared as follows.

[0086] 1. Preparation of mixed concentrate and complex golden extract

[0087] A mixed concentrate was prepared by hot water extraction and concentration of 28 types of medicinal herbs, including Poria cocos, Acorus calamus rhizome, Polygala tenuifolia root, and Longan husk, respectively, using conventional methods, and a complex Scutellaria baicalensis extract was prepared by adding dextrin and lactic acid to Scutellaria baicalensis concentrate, Glycyrrhiza glabra concentrate, Jujube concentrate, and Astragalus membranaceus concentrate.

[0088] 2. Preparation of the herbal medicine composition of Example 1

[0089] To purified water, 5 parts by weight of red ginseng concentrate, 20 parts by weight of mixed concentrate (7.74 parts by weight of Poria cocos, 7.74 parts by weight of Acorus calamus rhizome, 7.74 parts by weight of Polygala tenuifolia root, 7.74 parts by weight of Longan false seed husk, 2.58 parts by weight of Poria cocos, 2.58 parts by weight of Rehmannia glutinosa, 5.16 parts by weight of Cuscuta japonica seeds, 2.58 parts by weight of Cornus officinalis fruit, 1.94 parts by weight of Rubus coreanus, 7.74 parts by weight of turmeric extract, 2.58 parts by weight of Gastrodia elata root, 2.58 parts by weight of Paeonia lactiflora root, 2.58 parts by weight of Cinnamon, 2.58 parts by weight of Astragalus membranaceus, 2.58 parts by weight of Cnidium officinale rhizome, 2.58 parts by weight of Panax ginseng, 2.58 parts by weight of Eucommia ulmoides leaves, 2.58 parts by weight of Schisandra chinensis fruit, Lycium chinense fruit 2.58 parts by weight of yam root, 2.58 parts by weight of Ophiopogon japonicus root, 2.58 parts by weight of Rehmannia glutinosa, 2.58 parts by weight of Asparagus cochinchinensis tuberous root, 2.58 parts by weight of Glycyrrhiza glabra, 2.58 parts by weight of Angelica gigas root, 3.23 parts by weight of ginger, 3.23 parts by weight of Jujube, and 1.3 parts by weight of Cynanchum wilfordii tuberous root, converted based on 100 parts by weight of mixed concentrate), 18.3 parts by weight of fructooligosaccharide, 10 parts by weight of pear concentrate, 5 parts by weight of sugar syrup honey, 2 parts by weight of deer antler extract, and 0.3 parts by weight of complex Scutellaria baicalensis extract (36.8 parts by weight of Scutellaria baicalensis concentrate, 27.2 parts by weight of Glycyrrhiza glabra concentrate, 8.8 parts by weight of Jujube concentrate, 7.2 parts by weight of Astragalus membranaceus concentrate, 10 parts by weight of dextrin, and lactic acid A liquid herbal medicine composition (Example 1) was prepared by sequentially adding 10 parts by weight (amount converted based on 100 parts by weight of complex Scutellaria extract) and stirring at 65°C for 30 minutes to mix uniformly. Purified water was added as the remainder so that the total composition was 100 parts by weight (39.4 parts by weight).

[0090] 3. Preparation of herbal medicine compositions of Comparative Examples 1 to 13

[0091] The herbal medicine compositions of Comparative Examples 1 to 13 were prepared in the same manner as in Example 1 above, but as described in Table 1 below, by (1) changing the mixing ratio of the eight main components to be below the lower limit of a specific numerical range or above the upper limit, (2) excluding some of the internal components of the mixed concentrate or complex golden extract, or (3) changing the weight ratio of red ginseng concentrate and deer antler extract.

[0092] Specifically, Comparative Example 7 excluded Poria cocos, Acorus calamus rhizome, Polygala tenuifolia root, and longan false seed husk (total 30.96 parts by weight) from the mixed concentrate, and reconstituted the remaining 24 types of herbal medicines according to the original mixing ratio based on 100 parts by weight of the mixed concentrate, and mixed with 11.21 parts by weight of turmeric extract, 7.47 parts by weight of Cuscuta japonica seeds, 4.68 parts by weight each of ginger and jujube, 2.81 parts by weight of Rubus coreanus, 1.88 parts by weight of Prunella vulgaris tuber, and 3.74 parts by weight each of Poria cocos, Rehmannia glutinosa, Cornus officinalis fruit, Gastrodia elata root, Paeonia lactiflora root, Cinnamon, Astragalus membranaceus, Cnidium officinale rhizome, Panax ginseng, Eucommia ulmoides leaves, Schisandra chinensis fruit, Lycium barbarum fruit, Dioscorea japonica root, Ophiopogon japonicus root, Rehmannia glutinosa raw, Asparagus cochinchinensis tuber, Glycyrrhiza glabra, and Angelica gigas root.

[0093] Comparative Example 8 was prepared by excluding the scutellaria concentrate (36.8 parts by weight) from the complex scutellaria extract and reconstituting the remaining five ingredients according to the original formulation ratio based on 100 parts by weight of the complex scutellaria extract, and mixing 43.0 parts by weight of licorice concentrate, 13.9 parts by weight of jujube concentrate, 11.4 parts by weight of Astragalus concentrate, 15.8 parts by weight of dextrin, and 15.8 parts by weight of lactic acid.

[0094] Comparative Example 9 was prepared by excluding the turmeric extract (7.74 parts by weight) from the mixed concentrate and reconstituting the remaining 27 types of herbal medicines according to the original mixing ratio based on 100 parts by weight of the mixed concentrate. The mixture contained 8.39 parts by weight each of Poria cocos, Acorus calamus rhizome, Polygala tenuifolia root, and longan false seed husk; 5.59 parts by weight of Cuscuta japonica seeds; 3.50 parts by weight each of ginger and jujube; 2.10 parts by weight of Rubus coreanus; 1.41 parts by weight of Prunella vulgaris tuber; and 2.80 parts by weight each of Poria cocos, Rehmannia glutinosa, Cornus officinalis fruit, Gastrodia elata root, Paeonia lactiflora root, Cinnamon, Astragalus membranaceus, Cnidium officinale rhizome, Panax ginseng, Eucommia ulmoides leaves, Schisandra chinensis fruit, Lycium barbarum fruit, Dioscorea root, Ophiopogon japonicus root, Rehmannia glutinosa raw, Asparagus cochinchinensis tuber, Glycyrrhiza glabra, and Angelica gigas root.

[0095] division Red ginseng concentrate (parts by weight) purified water (parts by weight) Mixed concentrate (parts by weight) Deer antler extract (parts by weight) Complex golden extract (parts by weight) Red ginseng:deer antler weight ratio Example 1 5 39.4 20 2 0.3 1 : 0.4 Comparative Example 1 1 43.4 20 2 0.3 1 : 2.0 Comparative Example 2 12 32.4 20 2 0.3 1 : 0.17 Comparative Example 3 5 49.4 10 2 0.3 1 : 0.4 Comparative Example 4 5 29.4 30 2 0.3 1 : 0.4 Comparative Example 5 5 39.65 20 2 0.05 1 : 0.4 Comparative Example 6 5 37.7 20 2 2 1 : 0.4 Comparative Example 7 5 39.4 20 2 0.3 1 : 0.4 Comparative Example 8 5 39.4 20 2 0.3 1 : 0.4 Comparative Example 9 5 39.4 20 2 0.3 1 : 0.4 Comparative Example 10 5 59.4 0 2 0.3 1 : 0.4 Comparative Example 11 5 39.7 20 2 0 1 : 0.4 Comparative Example 12 3 40.4 20 3 0.3 1 : 1.0 Comparative Example 13 6 39.4 20 1 0.3 1 : 0.17

[0097] Experimental Example 1: Confirmation of memory improvement effect according to the mixing ratio of herbal medicine compositions

[0098] In order to confirm the effect of the mixing ratio of the eight main components of the herbal medicine composition according to the present invention and the weight ratio of red ginseng concentrate and deer antler extract on memory improvement, an experiment was conducted as follows using an animal model in which memory impairment was induced by scopolamine.

[0099] 1-1. Sample Preparation

[0100] The herbal medicine compositions of Example 1, Comparative Examples 1 to 6, and Comparative Examples 10 to 13 prepared according to Table 1 above were used as samples.

[0101] 1-2. Preparation of Animal Model for Memory Impairment and Sample Administration

[0102] 7-week-old ICR mice ( Mus musculus) were divided into groups of 10 individuals each for normal control, injury-induced control, positive control, Example 1, and Comparative Examples 1 to 6, and 10 to 13. Memory impairment was induced in all groups except the normal control by intraperitoneal administration of scopolamine 1 mg / kg once daily for 14 days, while simultaneously, the herbal medicine compositions of the Examples and Comparative Examples were orally administered at a dose of 200 mg / kg, and donepezil 5 mg / kg was orally administered once daily for 14 days to the positive control group. Equal volumes of distilled water were orally administered to the normal control and injury-induced control groups.

[0103] 1-3. Passive avoidance test

[0104] After administration was completed, an acquisition trial was conducted using a passive avoidance test device consisting of a light room and a dark room, and after 24 hours, the time from placing the mouse in the light room until moving it to the dark room (step-through latency, seconds) was measured (maximum measurement time limited to 300 seconds).

[0105] 1-4. Y-maze test

[0106] After placing a mouse in a Y-shaped maze device and allowing it to explore freely for 8 minutes, the percentage of spontaneous alternation (the behavior of entering three different branches in sequence) relative to the total number of maze entries was calculated.

[0107] 1-5. Experimental Results

[0108] division Step-through latency (seconds) Voluntary alternating behavior rate (%) Normal control group 280.4 ± 12.1 75.2 ± 3.4 Damage-inducing control group 45.2 ± 8.3 42.1 ± 4.1 Positive control (donepezil) 230.6 ± 15.7 68.3 ± 3.9 Example 1 245.8 ± 14.2 70.5 ± 3.6 Comparative Example 1 120.3 ± 11.5 52.4 ± 4.0 Comparative Example 2 148.9 ± 13.0 54.8 ± 3.8 Comparative Example 3 110.7 ± 10.8 50.2 ± 4.3 Comparative Example 4 158.4 ± 12.6 56.1 ± 3.7 Comparative Example 5 129.5 ± 11.9 53.0 ± 4.2 Comparative Example 6 138.2 ± 12.3 54.3 ± 3.9 Comparative Example 10 94.6 ± 9.7 47.9 ± 4.4 Comparative Example 11 124.1 ± 10.4 51.6 ± 4.1 Comparative Example 12 154.7 ± 13.4 57.2 ± 3.5 Comparative Example 13 144.3 ± 12.0 55.0 ± 3.7

[0109] As can be seen in Table 2 above, compared to the normal control group, the injury-induced control group showed a step-through latency of 235.2 seconds and a voluntary alternating behavior rate of 33.1%p, confirming that memory impairment induced by scopolamine was induced normally. In contrast, Example 1, formulated within the optimal numerical range, showed a step-through latency of 200.6 seconds and a voluntary alternating behavior rate of 28.4%p compared to the injury-induced control group, demonstrating a memory improvement effect equivalent to or greater than that of the positive control group, donepezil (latency 230.6 seconds, alternating behavior rate 68.3%).

[0110] On the other hand, Comparative Examples 1 to 6, in which the content of the eight main components was below the lower limit of the optimal numerical range or exceeded the upper limit, showed some recovery effect compared to the damage-inducing control group (latency 110.7 to 158.4 seconds), but only at a level 87.4 to 135.1 seconds lower than Example 1, confirming that a significant difference in effect occurred around the boundary of the numerical range. In addition, Comparative Examples 10 and 11, which did not contain any mixed concentrate or complex golden extract, showed the lowest recovery levels with latencies of 94.6 seconds and 124.1 seconds, respectively, confirming that both components are not merely additional components but contribute essentially to the manifestation of memory improvement effects. In addition, when the weight ratio of red ginseng concentrate to deer antler extract was changed from 1:0.4 in Example 1 to 1:1.0 (Comparative Example 12) or 1:0.17 (Comparative Example 13), the latency decreased by 91.1 seconds and 101.5 seconds, respectively, confirming that a significant synergistic effect is exhibited only at specific weight ratios even within the same individual content range.

[0111] Through the above results, it was confirmed that cholinergic nervous system protection and cognitive function-related behavioral indicators are significantly improved only when eight main components are mixed within an optimal numerical range, as in the herbal medicine composition of the present invention, and red ginseng concentrate and deer antler extract are combined in a specific weight ratio, and that this is due not to the simple summation effect of each component, but to a heterogeneous and significant synergistic effect based on the mixing ratio and weight ratio.

[0113] Experimental Example 2: Confirmation of Cholinergic Nervous System Protective Effect According to the Composition of the Herbal Medicine

[0114] In order to confirm the contribution of individual herbal ingredients included in the mixed concentrate and complex Scutellaria extract in the herbal medicine composition according to the present invention to the protective effect on the cholinergic nervous system, acetylcholinesterase (AChE) activity and acetylcholine (ACh) content in hippocampal tissue were measured.

[0115] 2-1. Sample Preparation

[0116] The herbal medicine compositions of Example 1 and Comparative Examples 7 to 11 prepared according to Table 1 above were used as samples.

[0117] 2-2. Preparation of Animal Model and Sample Administration

[0118] In the same manner as in Experimental Example 1 above, a scopolamine-induced memory impairment model was constructed for a normal control group, an injury-inducing control group, a positive control group (donepezil 5 mg / kg), and each sample group (200 mg / kg), and was orally administered for 14 days.

[0119] 2-3. Measurement of AChE activity in the hippocampus

[0120] After the administration was completed, mice were sacrificed, hippocampal tissue was extracted and homogenized, and then AChE activity (U / mg protein) with acetylthiocholine as a substrate was measured using the Ellman method. Using the AChE activity of the injury-induced control group as the standard (inhibition rate 0%), the AChE activity inhibition rate (%) of each sample group was calculated.

[0121] 2-4. Measurement of ACh content in the hippocampus

[0122] The ACh content (ng / mg protein) was quantified from the homogenized hippocampus tissue using an ELISA kit.

[0123] 2-5. Experimental Results

[0124] division AChE activity (U / mg protein) AChE activity inhibition rate (%) ACh content (ng / mg protein) Normal control group 3.2 ± 0.3 - 12.8 ± 0.9 Damage-inducing control group 8.5 ± 0.6 0 4.1 ± 0.5 Positive control (donepezil) 4.0 ± 0.4 52.9 10.5 ± 0.8 Example 1 4.2 ± 0.4 50.6 10.8 ± 0.7 Comparative Example 7 6.5 ± 0.5 23.5 7.2 ± 0.6 Comparative Example 8 6.8 ± 0.5 20.0 6.9 ± 0.6 Comparative Example 9 5.8 ± 0.5 31.8 7.9 ± 0.7 Comparative Example 10 7.6 ± 0.6 10.6 6.0 ± 0.6 Comparative Example 11 7.2 ± 0.5 15.3 6.5 ± 0.6

[0125] As can be seen in Table 3 above, Example 1 showed an AChE activity inhibition rate of 50.6% compared to the injury-inducing control group, demonstrating a cholinergic neuroprotective effect equivalent to that of the positive control group, donepezil (inhibition rate 52.9%).

[0126] In this regard, Comparative Example 7, which excluded four types of medicinal herbs—Poria cocos, Acorus calamus rhizome, Polygala tenuifolia root, and Longan husk—from the mixed concentrate, showed an inhibition rate of 23.5%, a decrease of 27.1%p compared to Example 1, and Comparative Example 8, which excluded Scutellaria baicalensis concentrate from the complex Scutellaria baicalensis extract, showed an inhibition rate of 20.0%, a decrease of 30.6%p compared to Example 1, confirming that the above components are key contributing factors to the protective effect on the cholinergic nervous system. Meanwhile, Comparative Example 9, which excluded only Turmeric extract from the mixed concentrate, showed an inhibition rate of 31.8%, a relatively smaller decrease (a decrease of 18.8%p compared to Example 1) than Comparative Examples 7 and 8, confirming that the contribution of Turmeric extract is relatively smaller than that of the above four medicinal herbs and Scutellaria baicalensis concentrate. In addition, Comparative Examples 10 and 11, which completely excluded the mixed concentrate or complex golden extract, showed the lowest levels of inhibition rates of 10.6% and 15.3%, respectively, confirming that the effect was significantly reduced compared to Comparative Examples 7 to 9, in which only some components were omitted. The ACh content also showed the same trend, with Example 1 (10.8 ng / mg protein) maintaining a significantly higher level compared to Comparative Examples 7 to 11 (6.0 to 7.9 ng / mg protein).

[0127] Through the above results, it was confirmed that the Poria cocos, Acorus calamus rhizome, Polygala tenuifolia root, and Longan husk in the mixed concentrate included in the herbal medicine composition of the present invention, and the Scutellaria baicalensis concentrate in the complex Scutellaria baicalensis extract organically combine to contribute to the expression of a protective effect on the cholinergic nervous system through the inhibition of AChE activity and the maintenance of ACh content, and that the effect is significantly reduced when these components are deficient.

[0129] Experimental Example 3: Confirmation of changes in hippocampal neurotrophic factor expression according to the mixing ratio of mixed concentrate and complex goldenrod extract

[0130] To determine the extent to which the key herbal ingredients included in the mixed concentrate and complex goldenrod extract contribute to the expression of hippocampal neurotrophic factors, changes in the expression of brain-derived neurotrophic factor (BDNF) and its receptor, TrkB (tropomyosin receptor kinase B), were measured.

[0131] 3-1. Sample Preparation

[0132] The herbal medicine compositions of Example 1 and Comparative Examples 7 to 9 prepared according to Table 1 above were used as samples.

[0133] 3-2. Preparation of Animal Model and Sample Administration

[0134] Scopolamine-induced memory impairment models were constructed for the normal control group, injury-induced control group, positive control group (donepezil 5 mg / kg), Example 1, and Comparative Examples 7 to 9 in the same manner as in Experimental Example 1, and each sample was orally administered at a dose of 200 mg / kg for 14 days.

[0135] 3-3. Measurement of BDNF and TrkB Expression in the Hippocampus

[0136] After the administration was completed, mice were sacrificed and hippocampal tissue was extracted, and protein expression of BDNF and TrkB was measured using Western blot. Protein expression levels were corrected using β-actin as an internal standard, and the relative expression levels (fold change) of each group were calculated by setting the expression level of the injury-induced control group to 1.00.

[0137] 3-4. Experimental Results

[0138] division BDNF relative expression level (fold change) TrkB relative expression level (fold change) Normal control group 2.85 ± 0.18 2.40 ± 0.15 Damage-inducing control group 1.00 ± 0.10 1.00 ± 0.09 Positive control (donepezil) 2.10 ± 0.16 1.85 ± 0.13 Example 1 2.25 ± 0.17 1.95 ± 0.14 Comparative Example 7 1.65 ± 0.14 1.45 ± 0.12 Comparative Example 8 1.58 ± 0.13 1.40 ± 0.11 Comparative Example 9 1.85 ± 0.15 1.60 ± 0.13

[0139] As can be seen in Table 4 above, Example 1 showed a 1.25-fold increase in BDNF expression and a 0.95-fold increase in TrkB expression compared to the injury-inducing control group, demonstrating a superior effect in increasing neurotrophic factor expression even compared to the positive control group, donepezil (1.10-fold increase in BDNF and 0.85-fold increase in TrkB).

[0140] In this regard, Comparative Example 7, which excluded four types of mixed concentrates—Poria cocos, Acorus calamus rhizome, Polygala tenuifolia root, and Longan husk—showed BDNF expression that was only 0.60 times lower than Example 1, and Comparative Example 8, which excluded the Scutellaria baicalensis concentrate from the complex Scutellaria baicalensis extract, showed the largest decrease in BDNF expression, which was 0.67 times lower than Example 1. Meanwhile, Comparative Example 9, which excluded only the Turmeric extract from the mixed concentrate, showed BDNF expression that decreased by only 0.40 times compared to Example 1, exhibiting a relatively smaller decrease than Comparative Examples 7 and 8. This trend is consistent with the order of AChE activity inhibition rates confirmed in Experimental Example 2 (in the order of Example 1 → Comparative Example 9 → Comparative Example 7 → Comparative Example 8), and TrkB expression also showed the same trend.

[0141] Through the above results, it was confirmed that the poria cocos, Acorus calamus rhizome, Polygala tenuifolia root, Longan husk, and Scutellaria baicalensis concentrate in the mixed concentrate of the present invention contribute significantly not only to the protective effect on the cholinergic nervous system but also to the increase in the expression of hippocampal neurotrophic factors, and that when these components are deficient, both the protective mechanism of the cholinergic nervous system and the mechanism of promoting neurotrophic factor expression are reduced.

[0143] The various embodiments described above may be embodied in other specific forms without departing from the technical idea and essential features. Accordingly, the above detailed description should not be interpreted restrictively in all respects but should be considered exemplary. The scope of the various embodiments shall be determined by a reasonable interpretation of the appended claims, and all modifications within the equivalent scope of the various embodiments are included within the scope of the various embodiments. Furthermore, embodiments may be constructed by combining claims that do not have an explicit citation relationship in the patent claims, or they may be included as new claims through amendments made after filing.

Claims

Claim 1 A herbal medicine composition for improving memory comprising red ginseng concentrate, purified water, mixed concentrate, fructooligosaccharide, pear concentrate, royal jelly, deer antler extract, and complex Scutellaria extract as active ingredients, wherein the herbal medicine composition is mixed with 2 to 8 parts by weight of red ginseng concentrate, 35 to 45 parts by weight of purified water, 15 to 25 parts by weight of mixed concentrate, 15 to 20 parts by weight of fructooligosaccharide, 8 to 12 parts by weight of pear concentrate, 2 to 8 parts by weight of royal jelly, 1 to 3 parts by weight of deer antler extract, and 0.1 to 1 part by weight of complex Scutellaria extract, wherein the red ginseng concentrate and deer antler extract are included in a weight ratio of 1:0.2 to 0.6, and the mixed concentrate comprises Poria cocos, Acorus calamus rhizome, Polygala tenuifolia root, Longan false seed husk, Poria cocos, and Rehmannia glutinosa. A herbal medicine composition for improving memory, comprising seeds of *Cuscuta japonica*, fruit of *Cornus officinalis*, *Rubus coreanus*, turmeric extract, root of *Gastrodia elata*, root of *Paeonia lactiflora*, cinnamon, *Astragalus membranaceus*, rhizome of *Cnidium officinale*, ginseng, *Eucommia ulmoides* leaves, fruit of *Schisandra chinensis*, fruit of *Lycium barbarum*, root of *Dioscorea opposita*, root of *Ophiopogon japonicus*, *Rehmannia glutinosa*, tuberous root of *Asparagus cochinchinensis*, licorice, root of *Angelica gigas*, ginger, jujube, and tuberous root of *Ganoderma lucidum*, wherein the above-mentioned complex *Scutellaria baicalensis* extract is composed of *Scutellaria baicalensis* concentrate, licorice concentrate, jujube concentrate, *Astragalus membranaceus* concentrate, dextrin, and lactic acid. Claim 2 In claim 1, the mixed concentrate comprises 7 to 8 parts by weight of Poria cocos, 7 to 8 parts by weight of Acorus calamus rhizome, 7 to 8 parts by weight of Polygala tenuifolia root, 7 to 8 parts by weight of Longan false seed husk, 2 to 3 parts by weight of Poria cocos, 2 to 3 parts by weight of Rehmannia glutinosa, 5 to 6 parts by weight of Cuscuta japonica seeds, 2 to 3 parts by weight of Cornus officinalis fruit, 1.5 to 2 parts by weight of Rubus fructus, 7 to 8 parts by weight of Turmeric extract, 2 to 3 parts by weight of Gastrodia elata root, 2 to 3 parts by weight of Paeonia lactiflora root, 2 to 3 parts by weight of Cinnamon, 2 to 3 parts by weight of Astragalus membranaceus, 2 to 3 parts by weight of Cnidium officinale rhizome, 2 to 3 parts by weight of Panax ginseng, 2 to 3 parts by weight of Eucommia ulmoides leaves, 2 to 3 parts by weight of Schisandra chinensis fruit, 2 to 3 parts by weight of Lycium chinense fruit, and Dioscorea japonica root. A herbal medicine composition for improving memory, comprising 2 to 3 parts by weight of Ophiopogon japonicus root, 2 to 3 parts by weight of Rehmannia glutinosa root, 2 to 3 parts by weight of Asparagus cochinchinensis tuberous root, 2 to 3 parts by weight of Glycyrrhiza glabra root, 2 to 3 parts by weight of Angelica gigas root, 3 to 4 parts by weight of ginger, 3 to 4 parts by weight of Jujube, and 1 to 1.5 parts by weight of Cynanchum wilfordii tuberous root, wherein the complex Scutellaria baicalensis extract comprises 35 to 40 parts by weight of Scutellaria baicalensis concentrate, 25 to 30 parts by weight of Glycyrrhiza glabra concentrate, 7 to 10 parts by weight of Jujube concentrate, 6 to 8 parts by weight of Astragalus membranaceus concentrate, 8 to 12 parts by weight of dextrin, and 8 to 12 parts by weight of lactic acid. Claim 3 A herbal medicine composition for improving memory according to claim 1, wherein the herbal medicine composition increases the expression of brain-derived neurotrophic factor (BDNF) compared to a control group not treated with the herbal medicine composition in an animal model in which memory impairment is induced. Claim 4 A health functional food composition for improving memory, comprising a herbal medicine composition according to any one of claims 1 to 3. Claim 5 (a) a step of obtaining a mixed concentrate by extracting and concentrating Poria cocos, Acorus calamus rhizome, Polygala tenuifolia root, Longan false seed husk, Poria cocos, Rehmannia glutinosa, Cuscuta japonica seeds, Cornus fruit, Rubus fructus, Turmeric extract, Gastrodia elata root, Paeonia root, Cinnamon, Astragalus membranaceus, Cnidium officinale rhizome, Panax ginseng, Eucommia ulmoides leaves, Schisandra chinensis fruit, Lycium barbarum fruit, Dioscorea root, Ophiopogon japonicus root, Rehmannia glutinosa raw, Asparagus cochinchinensis tuberous root, Glycyrrhiza glabra, Angelica gigas root, Ginger, Jujube, and Cynanchum tuberous root; (b) a step of obtaining a complex Scutellaria baicalensis extract by mixing dextrin and lactic acid with Scutellaria baicalensis concentrate, Glycyrrhiza glabra concentrate, Jujube concentrate, and Astragalus membranaceus concentrate; and (c) a step of mixing red ginseng concentrate, purified water, fructooligosaccharide, pear concentrate, sugar syrup honey, and deer antler extract with the mixed concentrate of step (a) and the complex goldenrod extract of step (b); wherein step (c) comprises mixing 2 to 8 parts by weight of red ginseng concentrate, 35 to 45 parts by weight of purified water, 15 to 25 parts by weight of mixed concentrate, 15 to 20 parts by weight of fructooligosaccharide, 8 to 12 parts by weight of pear concentrate, 2 to 8 parts by weight of sugar syrup honey, 1 to 3 parts by weight of deer antler extract, and 0.1 to 1 part by weight of complex goldenrod extract.