Collagen-contaning liquid composition and method for preparing the same

Abstract

The present invention relates to a collagen-containing liquid composition and a method for manufacturing the same. More specifically, the collagen-containing liquid composition includes black carrot extract and / or prune extract, which are components capable of preventing color change of the composition itself due to external environments such as light. Therefore, even when the collagen-containing liquid composition is applied to a recyclable transparent PET container, it can be stored stably with minimal color change.

Description

Technology Field

[0001] The present invention relates to a collagen-containing liquid composition and a method for preparing the same. Background Technology

[0002] Since collagen plays a role in skin hydration, it is known that consuming collagen can prevent skin aging, joint weakening, and blood vessel damage. Utilizing these benefits, various functional products using collagen as a raw material are being developed.

[0003] Generally, liquid collagen products are primarily manufactured and sold in plastic containers.

[0004] The plastic containers currently in use are made of PET-PEN (polyethylene terephthalate-polyethylene naphthalate) material, which can block light and thus stably store contents in liquid form. Therefore, they were mainly used as containers for liquid products, but there was a problem with them not being recyclable.

[0005] It was confirmed that if the above PET-PEN material container is changed to a recyclable transparent PET container, the plastic container becomes recyclable and the amount of plastic used is reduced by about 65% compared to the existing one, so there was an attempt to change the existing plastic ampoule container to a transparent PET container.

[0006] However, when applying liquid collagen products to transparent PET containers, a problem arose in which the color of the contents inside the PET container changed significantly due to light.

[0007] Accordingly, there is a need to develop a liquid collagen product that exhibits excellent stability even when applied to recyclable transparent PET containers, thereby preventing color change caused by light. Prior art literature

[0008] Japanese Registered Patent No. 6296792 The problem to be solved

[0009] To solve the above problem, the inventors confirmed that when black carrot extract and / or prune extract are applied to a collagen-containing liquid composition, discoloration caused by external environments such as sunlight is minimized, thereby enhancing the stability of the collagen-containing liquid composition.

[0010] Accordingly, the objective of the present invention is to provide a collagen-containing liquid composition with enhanced stability and a method for manufacturing the same. means of solving the problem

[0011] To achieve the above objective, the present invention provides a collagen-containing liquid composition comprising (i) collagen; and (ii) one or more selected from black carrot extract and prune extract. Effects of the invention

[0012] The collagen-containing liquid composition according to the present invention, due to its composition including black carrot extract and / or prune extract, minimizes discoloration of the liquid composition caused by external environments such as sunlight, thereby exhibiting the effect of enhanced stability. Brief explanation of the drawing

[0013] Figure 1 shows the color difference of the liquid compositions prepared in the examples and comparative examples according to storage time. Specific details for implementing the invention

[0014] The present invention will be described in more detail below.

[0016] Collagen-containing liquid composition

[0017] The present invention relates to a collagen-containing liquid composition comprising (i) collagen; and (ii) one or more selected from black carrot extract and prune extract. When the collagen-containing liquid composition includes both black carrot extract and prune extract, the discoloration prevention effect of the liquid composition may be significantly improved.

[0019] In the present invention, the collagen plays a role in providing strength and tensile strength to the skin and protecting the skin, and is related to skin beauty and anti-aging. Animal collagen, fish collagen, etc., used in oral collagen products can be widely used.

[0020] For example, the above collagen may be fish collagen, and the absorption rate may be better when using fish collagen.

[0021] In addition, the collagen may be a collagen peptide, and the collagen peptide may have an average molecular weight of 1000 Da to 2000 Da, specifically 1000 Da or more, 1200 Da or more, or 1400 Da or more, 1600 Da or less, 1800 Da or less, or 2000 Da or less, and preferably 1500 Da.

[0022] In addition, the collagen contains glycine-proline-hydroxyproline (GPH), and the GPH may be included in an amount of 2.5% by weight or more based on the total weight of the collagen. Specifically, the content of the GPH may be 2.5% by weight or more, 2.8% by weight or more, 3.0% by weight or more, 3.2% by weight or more, or 3.4% by weight or more. If the content falls within the above range, there may be an advantage of excellent absorption into the body upon oral intake. The upper limit of the GPH content is not specifically restricted, but may be 4.2% by weight or less.

[0023] The above collagen peptide is a complex comprising three amino acids, including "glycine-xy," wherein x and y can be any amino acid, and x and y can be the same or different amino acids. That is, commonly naturally occurring alanine (Ala), valine (Val), leucine (Leu), isoleucine (Ile), proline (Pro), hydroxyproline (Hyp), phenylalanine (Phe), tryptophan (Trp), methionine (Met), serine (Ser), threonine (Thr), cysteine ​​(Cys), glutamine (Gln), glycine (Gly), asparagine (Ason), tyrosine (Tyr), lysine (Lys), arginine (Arg), histidine (His), aspartic acid (Asp), and glutamic acid (Glu) All amino acids such as the above may be used, but preferably, the composition may be Gly-Pro-Hyp, Gly-Pro-Ala, Gly-Ala-Hyp, Gly-Leu-Hyp, Gly-Glu-Lys, Gly-Pro-Lys, Gly-Glu-Hyp, Gly-Phe-Hyp, Gly-Ser-Hyp, Gly-Gln-Hyp, and Gly-Glu-Arg, Gly-Pro-Arg, but preferably, it may be glycine-proline-hydroxyproline (GPH) linked.

[0024] In addition, the collagen may be included in an amount of 0.1% to 10% by weight based on the total weight of the composition. Specifically, the content of the collagen may be 0.1% or more, 1% or more, 2% or more, or 3% or more by weight, and 6% or less, 8% or less, or 10% or less by weight. If the content of the collagen is less than 0.1% by weight, the inherent effects of collagen, such as the enhancement of skin elasticity, may not be significantly apparent, and if it exceeds 10% by weight, the preference for taste may decrease.

[0026] In the present invention, the black carrot extract can be obtained by extracting or juicing one or more of the leaves, stems, flowers, and fruits of the black carrot.

[0027] The above black carrot contains a large amount of anthocyanin. The anthocyanin is a pigment found in flowers or fruits, and when the black carrot extract is applied to a collagen-containing liquid composition, the anthocyanin acts as a light-blocking agent to prevent discoloration of the collagen-containing liquid composition, thereby improving stability.

[0028] Additionally, the black carrot extract may be in the form of a black carrot concentrate, and the black carrot concentrate may have a concentration of 60 Brix to 75 Brix. Specifically, the sugar content of the black carrot concentrate may be 60 Brix or higher, 62 Brix or higher, or 64 Brix or higher, or 71 Brix or lower, 73 Brix or lower, or 75 Brix or lower. If the sugar content of the black carrot concentrate is less than 60 Brix, the discoloration prevention effect of the collagen-containing liquid composition may be negligible, and if it exceeds 75 Brix, the formulation stability and sensory quality of the raw material may be degraded.

[0029] Additionally, the black carrot extract may be included in an amount of 0.1% to 5% by weight based on the total weight of the composition. Specifically, the content of the black carrot extract may be 0.1% or more by weight, 0.3% or more by weight, 0.5% or more by weight, or 1.0% or more by weight, and may be 1.8% or less by weight, 2.0% or less by weight, 3.0% or less by weight, 4.0% or less by weight, or 5.0% or less by weight. If the content of the black carrot extract is less than 0.1% by weight, the light-blocking effect against external environments such as sunlight may not be significantly present, and if it exceeds 5.0% by weight, the formulation stability and sensory quality of the product may be reduced.

[0031] In the present invention, the prune extract can be obtained by extracting or juicing the fruit of a prune, and preferably, by extracting or juicing the flesh portion surrounding the seed of the fruit of a prune.

[0032] The above-mentioned prunes contain a large amount of anthocyanin. The anthocyanin is a pigment found in flowers or fruits, and when the prune extract is applied to a collagen-containing liquid composition, the anthocyanin acts as a light-blocking agent, preventing discoloration of the collagen-containing liquid composition and improving its stability.

[0033] In addition, the prune extract may be in the form of a prune concentrate, and the prune concentrate may have a concentration of 65 Brix to 75 Brix. Specifically, the sugar content of the prune concentrate may be 65 Brix or higher, 66 Brix or higher, or 67 Brix or higher, or 73 Brix or lower, 74 Brix or lower, or 75 Brix or lower. If the sugar content of the prune concentrate is less than 60 Brix, the discoloration prevention effect of the collagen-containing liquid composition may be negligible, and if it exceeds 75 Brix, the formulation stability and sensory quality of the raw material may be degraded.

[0034] In addition, the prune extract may be included in an amount of 0.1% to 5% by weight based on the total weight of the composition. Specifically, the content of the prune extract may be 0.1% or more by weight, 0.3% or more by weight, 0.5% or more by weight, or 1.0% or more by weight, and may be 1.8% or less by weight, 2.0% or less by weight, 3.0% or less by weight, 4.0% or less by weight, or 5.0% or less by weight. If the content of the prune extract is less than 0.1% by weight, the light-blocking effect against external environments such as sunlight may not be significantly present, and if it exceeds 5.0% by weight, the formulation stability and sensory quality of the product may be reduced.

[0036] In the present invention, the color change of the collagen-containing liquid composition is a color difference (ΔE) defined by the following formula 1. * ab It is calculated as ), and the color difference may be 12 or less, specifically, 12 or less, 11 or less, 10 or less, 8 or less, or 7 or less:

[0037] <Equation 1>

[0038]

[0040] In the above Equation 1, L * 1, a * 1, b * 1 and L * 2, a * 2, b * 2 refers to colorimeter values ​​measured at different points in time at regular time intervals. For example, if the collagen-containing liquid composition is placed in a transparent PET container and stored in a sunny place for 12 weeks, L * 1, a * 1, b * 1 is the colorimeter value measured at week 0 (start of storage), and L * 2, a * 2, b * 2 may be the colorimeter value measured in week 12, and ΔE *ab The color difference may be a color change of the above collagen-containing liquid composition over 12 weeks.

[0041] The colorimeter mentioned in this specification is a “CIE-Lab colorimeter” defined by the International Commission on Illumination (CIE) and expressed as L*, a*, and b* (CIE-Lab values), where L*, a*, and b* represent the three characteristics of color: lightness, saturation, and hue. Specifically, L* represents lightness and is expressed as a value from 0 to 100. a* and b* are the planar coordinates (x,y) of the colorimeter, with a* on the horizontal axis and b* on the vertical axis. At this time, a* is the green-to-red spectrum, where +a approaches red and -a approaches green, and is expressed as a value from -120 to +120. In addition, b* represents the blue-to-yellow spectrum, where the +b side approaches yellow and the -b side approaches blue, and like a*, it is expressed with values ​​ranging from -120 to +120.

[0043] In the present invention, the pH of the collagen-containing liquid composition may be 3 to 4. Specifically, the pH may be 3 or higher, 3.2 or higher, or 3.4 or higher, and may be 3.6 or lower, 3.8 or lower, or 4.0 or lower. When such a pH range is satisfied, a discoloration effect due to light blocking is significantly exhibited, which can provide an advantageous effect in terms of stability.

[0045] In the present invention, the liquid composition may further include one or more additives selected from the group consisting of acidifiers, sweeteners, preservatives, antifoaming agents, suspending agents, antioxidants, stabilizers, and flavors.

[0046] The above acidity agent may be added in an appropriate amount to adjust acidity. Examples of the above acidity agents include citric acid, acetic acid, tartaric acid, malic acid, lactic acid, fumaric acid, phosphoric acid, and salts thereof, but are not limited thereto.

[0047] In addition, the above sweetener may be added in an appropriate amount to mask the bitter taste. Examples of the above sweeteners include, but are not limited to, xylitol, D-sorbitol, maltitol, mannitol, erythritol, lactose, glucose, oligosaccharide, fructose, refined sugar (sugar), aspartame, stevioside, enzyme-treated stevia, sucralose, and steviol glycosides.

[0048] In addition, the above-mentioned preservative may be added in an appropriate amount to improve preservation. Examples of the above-mentioned preservatives include dehydroacetic acids, sorbic acids, benzoic acids, propionic acids, and para-hydroxybenzoic acid esters, but are not limited thereto.

[0049] In addition, the above-mentioned antifoaming agent may be added in an appropriate amount to remove bubbles. Examples of the above-mentioned antifoaming agents include carnosic acid, lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid, etc., but are not limited thereto.

[0050] In addition, the above scent may be an air freshener. Examples of the air fresheners include, but are not limited to, orange oil, fruit juice extract, cinnamon oil, spearmint oil, peppermint oil, vanilla, peppermint oil, rose oil, lemon oil, berry scent, strawberry scent, grape scent, berry scent, yogurt scent, etc.

[0051] In addition, the liquid composition of the present invention may further include additives such as thickeners, volume expanders, or pH adjusters that are commonly used in food compositions.

[0053] In the present invention, the liquid composition may be a food composition or a health functional food composition.

[0054] In addition, the above liquid composition can be formulated into a beverage or drink.

[0055] In addition, since the above liquid composition can minimize discoloration caused by external environments such as sunlight even when packaged in a transparent PET container, the recycling rate of the transparent PET container can be increased.

[0057] Method for preparing a collagen-containing liquid composition

[0058] The present invention relates to a method for preparing a collagen-containing liquid composition, comprising the step of dissolving (i) collagen; and (ii) one or more selected from black carrot extract and prune extract; and may further comprise the step of dissolving an additive. At this time, the types and amounts of the collagen, black carrot extract, prune extract, and additive are as described above.

[0060] In the present invention, the black carrot extract may be prepared by undergoing the steps of grinding, extracting, and filtering black carrots, and a black carrot concentrate may be prepared by adding a concentration step. Alternatively, the black carrot concentrate may be prepared by extracting juice from the black carrots and then concentrating them. In this case, the extraction temperature may be 60°C or higher, 65°C or higher, or 70°C or higher, and may be 85°C or lower, 90°C or lower, or 95°C or lower. In addition, the extraction time may be 0.5 hours or higher or 1 hour or higher, and may be 2 hours or lower or 3 hours or lower. Furthermore, the extraction solvent may be one or more selected from the group consisting of water and alcohols having 1 to 5 carbon atoms, and preferably one or more selected from the group consisting of water and ethanol.

[0062] In the present invention, the prune extract may be prepared by grinding prunes and undergoing the steps of extraction and filtration, and a prune concentrate may be prepared by adding a concentration step. Alternatively, the prune concentrate may be prepared by extracting juice from the prunes and then concentrating it. At this time, the extraction temperature may be 60°C or higher, 65°C or higher, or 70°C or higher, and may be 85°C or lower, 90°C or lower, or 95°C or lower. In addition, the extraction time may be 0.5 hours or higher or 1 hour or higher, and may be 2 hours or lower or 3 hours or lower. In addition, the extraction solvent may be one or more selected from the group consisting of water and alcohols having 1 to 5 carbon atoms, and preferably one or more selected from the group consisting of water and ethanol.

[0064] Preferred embodiments are presented below to aid in understanding the present invention; however, the following embodiments are merely illustrative of the invention, and it is obvious to those skilled in the art that various changes and modifications are possible within the scope and spirit of the invention, and that such changes and modifications fall within the scope of the appended claims.

[0066] In the following examples and comparative examples, a collagen-containing liquid composition was prepared according to the composition listed in Table 1 below.

[0068] Raw Materials (Unit: Weight%) Example 1 Example 2 Example 3 Comparative Example 1 Collagen peptide 4.00 4.00 4.00 4.00 prune concentrate 1.50 2.00 0.00 0.00 Black Carrot Concentrate 0.50 0.00 2.00 0.00 incense 0.50 0.50 0.50 0.50 Sanmije Citric acid 0.15 0.15 0.15 0.15 sweetener xylitol 10.00 10.00 10.00 10.00 purified water 83.35 83.35 83.35 85.35 total 100.00 100.00 100.00 100.00

[0070] Example 1

[0071] According to the composition described in Table 1 above, collagen peptide (AP collagen enzymatic hydrolyzed peptide, Amorepacific Co., Ltd.), prune concentrate (prune concentrate, Taylor Brothers Farms), and black carrot concentrate (black carrot concentrate, Nature) were dissolved in purified water, and then flavor, citric acid, and xylitol were further dissolved to prepare a collagen-containing liquid composition.

[0073] Example 2

[0074] A collagen-containing liquid composition was prepared in the same manner as in Example 1, except that black carrot concentrate was not used.

[0076] Example 3

[0077] A collagen-containing liquid composition was prepared in the same manner as in Example 1, except that prune concentrate was not used.

[0079] Comparative Example 1

[0080] A collagen-containing liquid composition was prepared in the same manner as in Example 1, except that black carrot concentrate and prune concentrate were not used.

[0082] Experimental Example 1

[0083] The liquid compositions prepared in the examples and comparative examples were each placed in transparent PET containers and stored in a place exposed to sunlight. Using a spectrophotometer (Spectro Photometer CM-5, KONICA MINOLTA), the color (L) of the liquid compositions was measured at 0, 2, 4, 8, and 12 weeks after storage. * , a * , b * ) was measured.

[0084] The measured color was applied to Equation 1 below to calculate the color difference (ΔE*ab) according to the storage period.

[0085] <Equation 1>

[0086]

[0087] In the above Equation 1, L * 1, a * 1, b * 1 and L * 2, a * 2, b * 2 represents the colorimeter value measured over a set period of time.

[0089] Figure 1 shows the color difference of the liquid compositions prepared in the examples and comparative examples according to storage time.

[0090] Referring to FIG. 1, it can be seen that Example 1, which contains both black carrot concentrate and prune concentrate, has the smallest change in color difference over time. Specifically, it was confirmed that in Example 1, the color difference was 4 or less when stored for 2 weeks, 6 or less when stored for 4 weeks, 6 or less when stored for 8 weeks, and 7 or less when stored for 12 weeks.

[0091] Examples 2 and 3 are liquid compositions that do not contain black carrot concentrate and prune concentrate, respectively, compared to Example 1, and showed a relatively larger color difference over time compared to Example 1.

[0092] In addition, Comparative Example 1 is a liquid composition that does not contain either black carrot concentrate or prune concentrate, and it was found to have the largest color difference over time.

[0094] From these results, it was found that black carrot concentrate and prune concentrate play a role in preventing discoloration of the collagen-containing liquid composition, and in particular, the best discoloration prevention effect was observed when black carrot concentrate and prune concentrate were used together.

[0096] Although the present invention has been described above by limited embodiments and drawings, the present invention is not limited thereto, and it is obvious that various modifications and variations are possible within the scope of the technical spirit of the present invention and the equivalent scope of the claims described below by those skilled in the art to which the present invention belongs.

Claims

Claim 1 A collagen-containing liquid composition comprising (i) collagen; and (ii) one or more selected from black carrot extract and prune extract. Claim 2 A collagen-containing liquid composition according to claim 1, wherein the collagen is fish-derived collagen. Claim 3 A collagen-containing liquid composition according to claim 1, wherein the collagen has a glycine-proline-hydroxyproline (GPH) content of 2.5% to 5% by weight. Claim 4 A collagen-containing liquid composition according to claim 1, wherein the black carrot extract is included in an amount of 0.1% to 5% by weight based on the total weight of the composition. Claim 5 A collagen-containing liquid composition according to claim 1, wherein the prune extract is included in an amount of 0.1% to 5% by weight based on the total weight of the composition. Claim 6 A collagen-containing liquid composition according to claim 1, wherein the collagen is included in an amount of 0.1% to 10% by weight based on the total weight of the composition. Claim 7 In claim 1, the color change of the composition is a color difference (ΔE) defined by the following formula 1. * ab It is calculated as ), and the above color difference (ΔE * ab Liquid composition in which ) is 12 or less: <Formula 1> In the above Equation 1, L * 1, a * 1, b * 1 and L * 2, a * 2, b * 2 represents the colorimeter value measured over a set period of time. Claim 8 A collagen-containing liquid composition according to claim 1, wherein the composition has a pH of 3 to 4. Claim 9 A collagen-containing liquid composition according to claim 1, wherein the composition further comprises one or more additives selected from the group consisting of acidifiers, sweeteners, preservatives, antifoaming agents, suspending agents, antioxidants, stabilizers, and flavors. Claim 10 A collagen-containing liquid composition according to claim 1, wherein the composition is a food composition or a health functional food composition. Claim 11 A collagen-containing liquid composition according to claim 1, wherein the composition is formulated as a beverage or drink.

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