Cosmetic composition for preventing or improvement hair loss with Physalis Alkekengi Extract

Abstract

The present invention relates to a cosmetic composition for preventing or improving hair loss comprising Physalis extract. The cosmetic composition according to the present invention exhibits excellent hair loss alleviation and hair growth promotion efficacy by promoting the proliferation of hair papilla cells through intercellular hair proliferation signaling.

Description

Technology Field

[0001] The present invention relates to a cosmetic composition for preventing or improving hair loss comprising Physalis extract. Background Technology

[0003] Hair plays a role in protecting the human body from external stimuli, such as by protecting the scalp and maintaining body temperature. Hair follicles, where hair grows, are formed in the scalp in numbers ranging from approximately 100,000 to 150,000, and are characterized by a continuous repetition of cycles involving growth, regression, and resting phases throughout a lifetime. In particular, the dermal papilla cells that form the hair follicles play a crucial role in hair growth. Specifically, during the growth phase, the proliferation of the follicle's constituent cells surrounding the dermal papilla occurs actively; during the regression phase, follicle growth ceases, leading to apoptosis, condensation of the dermal papilla, and reorganization of the extracellular matrix; and during the resting phase, only the permanently persisting portion, including the raised area, remains in preparation for the follicle to re-enter the growth phase.

[0004] Hair loss refers to a phenomenon in which the proportion of hair growing in the regression or resting phase of the hair follicle growth cycle becomes higher than that growing in the growth phase, resulting in an abnormal increase in hair shedding. The hair cycle is reported to be altered or shortened by internal and external factors such as hormones, stress, disease, environmental pollution, and smoking, and that hair loss is caused by the complex interaction of these factors. Generally, the most typical form of hair loss is androgenic alopecia, which is caused by an excess of the male hormone testosterone. Testosterone is known to be reduced to dihydrotestosterone by reductase enzymes and is involved in hair loss, acne, and benign prostatic hyperplasia; in particular, dihydrotestosterone is known to induce hair loss by reducing the proliferation of hair follicle cells through the inhibition of protein synthesis around the hair follicle.

[0005] Treatments known to be effective for preventing hair loss or promoting hair growth to date include the application of minoxidil solution or the oral administration of tricosaccharide. Minoxidil was initially developed as a vasodilator for the treatment of hypertension, but it was later developed as a hair growth agent following reports of hypertrichosis as a side effect. The mechanism of action regarding the hair growth effect of minoxidil is believed to be the induction of hair growth through increased nutrient supply via vasodilation. In addition, finasteride is a substance that inhibits the activity of 5α-reductase, an enzyme involved in male hormone metabolism; it was initially developed as a treatment for benign prostatic hyperplasia, but was later developed as a hair growth agent following reports of hypertrichosis as a side effect.

[0006] However, since minoxidil and finasteride are chemically synthesized pharmaceutical preparations, they can cause unwanted side effects. Furthermore, they present the inconvenience of requiring continuous use because their effects disappear upon discontinuation; in the case of minoxidil, side effects such as a sticky sensation and skin irritation have been reported. In the case of finasteride, sexual dysfunction such as decreased libido and erectile dysfunction has been reported, which currently limits its continuous use. Consequently, there is an urgent need to develop hair growth agents composed of natural substances that provide efficacy equivalent to or greater than minoxidil or finasteride while having fewer side effects.

[0007] Accordingly, the inventors completed the present invention by confirming the effective proliferative ability of natural material extracts on hair papilla cells after making diligent efforts to resolve the aforementioned problems. Prior art literature

[0009] Korean Registered Patent No. 10-1017216 (February 16, 2011) relates to a hair growth agent composition and a method for manufacturing the same. Specifically, it discloses a hair growth agent composition obtained by mixing a mixture comprising 5 to 10 parts by weight of duck egg yolk, 10 to 20 parts by weight of duck eggshell, 10 to 20 parts by weight of white-flowered dandelion root or leaf, 10 to 20 parts by weight of *Ixeris dentata* root or leaf, 8 to 12 parts by weight of *Magnolia obovata* bark, 5 to 10 parts by weight of *Physalis longifolia* fruit, 5 to 10 parts by weight of *Imperata cylindrica* root, 1 to 5 parts by weight of pine needles, 1 to 5 parts by weight of *Opuntia ficus-indica* fruit, 1 to 5 parts by weight of Jerusalem artichoke, 1 to 5 parts by weight of black soybean, 1 to 5 parts by weight of black sesame, and 1 to 5 parts by weight of lotus root with water in a weight ratio of 1:1, boiling for 5 to 9 hours, and then distilling the mixture. Korean Registered Patent No. 10-1981678 (2019.05.17.) discloses a complex antioxidant extract and cosmetic composition for promoting hair growth and improving the scalp and hair, specifically a cosmetic composition comprising a mixed extract of aloe, prickly pear, schisandra, aronia, cordyceps, and cauliflower. The problem to be solved

[0010] The present invention aims to develop and provide a cosmetic composition for preventing hair loss made of natural materials having the efficacy of alleviating hair loss and promoting hair growth. means of solving the problem

[0012] The present invention provides a cosmetic composition for preventing or improving hair loss comprising a Physalis extract or a fraction thereof. In this case, the cosmetic composition may preferably further possess hair growth-promoting ability.

[0013] In the cosmetic composition of the present invention, the Physalis extract may preferably be extracted using any one solvent selected from purified water, lower alcohols having 1 to 4 carbon atoms, and mixed solvents thereof.

[0014] In the cosmetic composition of the present invention, the fraction may preferably be fractionated by additionally adding a solvent selected from hexane, methylene chloride, ethyl acetate, and butanol to a Physalis extract.

[0015] The cosmetic composition of the present invention may have any one formulation selected from the group consisting of, for example, shampoo, rinse, treatment, hair pack, hair gel, ampoule, hair tonic, essence, hair cream, mist, and soap. Effects of the invention

[0017] The cosmetic composition according to the present invention exhibits excellent efficacy in alleviating hair loss and promoting hair growth by promoting the proliferation of hair papilla cells through intercellular hair proliferation signaling. Brief explanation of the drawing

[0019] Figure 1 illustrates the process of isolating and purifying cynaroside, an indicator component, from Physalis extract. Figure 2 shows the results of qualitative analysis of cynaroside using high-speed liquid chromatography. Figure 3 is the screening result of the cynaroside content for the Physalis extracts and fractions thereof of Examples 1 to 5. Figure 4 shows the results of the evaluation of dermal papilla cell proliferation for the Physalis extract and fractions thereof of Examples 1 to 5. Figure 5 shows the results of confirming the level of IGF-1 (Insuline-like growth factor) expression in the Physalis extract of Example 1 and the fraction of Example 4. Figure 6 shows the results of confirming the degree of VEGF (Vascular endothelial growth factor) expression in the Physalis extract of Example 1 and the fraction of Example 4. Specific details for implementing the invention

[0020] In one embodiment of the present invention, it was confirmed that Physalis extract and its fractions exhibit hair loss alleviation and hair growth promotion effects by promoting the proliferation of hair papilla cells through intercellular hair proliferation signaling.

[0021] Accordingly, the present invention provides a cosmetic composition for preventing or improving hair loss comprising a Physalis extract or a fraction thereof. In this case, the cosmetic composition may preferably further possess hair growth-promoting ability.

[0022] ground cherry( Physalis alkekengi It is a perennial herb belonging to the Solanaceae family and is an ornamental plant grown in sunny, barren, and dry soil. Its roots and fruits are used for medicinal or culinary purposes. In traditional Korean medicine, the dried whole plant is called Sanjang and is prescribed for parasites, fever, gonorrhea, dysmenorrhea, eye diseases, lymphadenitis, hepatitis, cirrhosis, and uterine inflammation. It is known to have diuretic, analgesic, and detoxifying effects.

[0023] In the cosmetic composition of the present invention, the Physalis extract may preferably be extracted using any one solvent selected from purified water, lower alcohols having 1 to 4 carbon atoms, and mixed solvents thereof. As an example, an aqueous ethanol solution of 50 to 95% (v / v) may be used as the extraction solvent, and preferably, it may be extracted with a 70% aqueous ethanol solution.

[0024] In addition, the above Physalis extract may be prepared by adding about 1 to 20 times, preferably about 1 to 15 times, an extraction solvent to the raw Physalis material, and by heat extraction, cold maceration extraction, reflux cooling extraction, or ultrasonic extraction. Such extraction may also be performed under low temperature, room temperature, or high temperature conditions for 1 to 24 hours, preferably 3 to 7 hours.

[0025] In addition, the extract may be obtained by filtration and / or vacuum concentration, and the extraction process may be repeated 2 to 4 times and additionally subjected to processes such as filtration, concentration, and freeze-drying.

[0026] In the cosmetic composition of the present invention, the fraction may preferably be fractionated by adding any one solvent selected from hexane, methylene chloride, ethyl acetate, and butanol to a Physalis extract, preferably a powdered extract.

[0027] In particular, according to one embodiment of the present invention, excellent hair papilla cell proliferation ability was confirmed in the Physalis extract and its fractions prepared in the present invention, and in particular, in the Physalis extract and its ethyl acetate fraction, thereby confirming the efficacy of the extract and its fractions in preventing hair loss and promoting hair growth.

[0028] Meanwhile, in the cosmetic composition of the present invention, the Physalis extract or its fraction may be contained in an amount of 0.001 to 30 weight% relative to the total weight of the composition, and more specifically, in an amount of 0.001 to 10 weight%. If the content of the Physalis extract or its fraction is less than 0.001 weight%, the hair loss alleviation and hair growth promotion effects do not appear, and if it exceeds 30 weight%, the increase in hair loss alleviation and hair growth promotion effects with increasing content is negligible, making it uneconomical, and it is undesirable due to problems with the safety and stability of the formulation.

[0029] In addition to the Physalis extract or fractions thereof of the present invention as active ingredients, the ingredients included in the cosmetic composition of the present invention may also include other ingredients that can provide a synergistic effect to the main effect, provided that the main effect intended by the present invention is not impaired. For example, they may include conventional auxiliary agents such as antioxidants, stabilizers, solubilizers, vitamins, pigments, and fragrances, and carriers.

[0030] The cosmetic composition of the present invention may be prepared in any formulation conventionally manufactured in the art, for example, into a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, pack, massage cream, and spray, but is not limited thereto. More specifically, it may have any one formulation selected from the group consisting of shampoo, rinse, treatment, hair pack, hair gel, ampoule, hair tonic, essence, hair cream, mist, and soap.

[0031] In the case where the formulation of the cosmetic composition of the present invention is a paste, cream, or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide may be used as a carrier component.

[0032] When the formulation of the cosmetic composition of the present invention is a solution or an emulsion, a solvent, a solubilizing agent, or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, or fatty acid ester of sorbitan.

[0033] When the formulation of the cosmetic composition of the present invention is a suspension, a liquid diluent such as water, ethanol, or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracanthenic acid may be used as a carrier component.

[0034] When the formulation of the cosmetic composition of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, it may additionally include a propellant such as chlorofluorohydrocarbon, propane / butane, or dimethyl ether.

[0035] In the case where the formulation of the cosmetic composition of the present invention is a product containing a surfactant, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester, etc. may be used as a carrier component.

[0037] The contents of the present invention will be explained in more detail below through the following examples. However, the scope of the present invention is not limited to the following examples but includes variations of equivalent technical concepts.

[0039] [Example 1: Preparation of Physalis Extract]

[0040] 1 kg of Physalis was washed with clean water, dried, and ground to a size of 100 mesh. It was then reflux-extracted twice for 5 hours each with a 70% (v / v) aqueous ethanol solution and cold-macerated. Afterward, it was filtered through Whatman #4 filter paper and concentrated under reduced pressure to prepare 150 g of Physalis extract.

[0042] [ Examples 2 to 5: Physalis extract fractions purchase]

[0043] 150 g of the Physalis extract obtained in Example 1 above was suspended in purified water, and then solvent fractionation was performed in the order of hexane, methylene chloride, ethyl acetate, and butanol. Afterwards, each fraction was concentrated under reduced pressure to obtain 38 g of hexane fraction (Hex fraction; Example 2), 32 g of methylene chloride fraction (MC concentrate; Example 3), 14 g of ethyl acetate fraction (EA concentrate; Example 4), and 45 g of butanol fraction (BuOH concentrate; Example 5).

[0045] [Example 6: Isolation of cynaroside from the ethyl acetate fraction of Physalis extract]

[0046] Approximately 100 ml of a sample was prepared by dissolving the ethyl acetate fraction (EA concentrate) of the Physalis extract obtained in Example 4 in methanol. This was purified using a silica column and Sephadex LH-20 column chromatography to isolate cynaroside, and the isolated component was confirmed by HPLC analysis (Figs. 1 and 2). Fig. 1 illustrates the process of isolating and purifying cynaroside, an indicator component, from the Physalis extract, and Fig. 2 shows the results of qualitative analysis of cynaroside using high-speed liquid chromatography.

[0048] [Experimental Example 1: Component Analysis of Physalis Extract and Fractions]

[0049] HPLC was used to screen for cynaroside, an indicator component of the Physalis extract and its fractions prepared in Examples 1 to 5 above.

[0050] Specifically, about 1 g each of the Physalis extracts and fractions of Examples 1 to 5 was taken and dissolved in methanol to prepare 100 ml of a sample. This was filtered through a 0.45 micro syringe filter and used as the test solution.

[0051] The above test solution was analyzed according to the HPLC operating conditions shown in Table 1.

[0052] detector UV absorption spectrophotometer (260 nm) column A stainless steel tube with an inner diameter of approximately 4.6 mm and a length of approximately 25 cm filled with 5 µm octadecyl silylated silica gel for high-performance liquid chromatography. column temperature 30℃ flux 1.0 mL / min Sample injection volume 10 ㎕ Mobile phase Acetonitrile (A) · 0.1% aqueous formic acid solution (B) Gradient hour A (%) B (%) 0 15 85 5 15 85 20 25 75 21 90 10 27 90 10 28 15 85 35 15 85

[0054] As a result of the experiment, cynaroside, an indicator component of Physalis extract, was identified in all Physalis extracts and fractions of Examples 1 to 5, and in particular, Examples 4 and 5 were found to contain the highest content of cynaroside (Fig. 3). Fig. 3 is the screening result of the cynaroside content for the Physalis extracts and fractions thereof of Examples 1 to 5.

[0056] [Experimental Example 2: Evaluation of Hair Papilla Cell Proliferation]

[0057] 1×10⁶ human follicle dermal papilla cells (HFDPCs) 4 Cells were dispensed into a 96-well plate at a concentration of cells / mL and stabilized for 24 hours in a 37°C, 5% CO2 cell culture incubator. After replacing the medium with DMEM containing 1% FBS, Examples 1 to 5 were treated at different concentrations (3, 10, 30, 100 μg / mL), respectively, and reacted for 48 hours.

[0058] Subsequently, 20 µl of MTT reagent at a concentration of 5 mg / ml was added to each well, and the reaction was carried out for 2 hours in a 37°C, 5% CO2 cell culture incubator. After the reaction was complete, the supernatant from each well was completely removed, and 100 µl of DMSO was added to completely lyse the cells. Then, the absorbance was measured at 550 nm to calculate the cell proliferation rate of each sample group.

[0059] As a result of the experiment, in particular, the Physalis extract of Example 1 showed a cell proliferation effect of approximately 113.0% at a concentration of 30 μg / ml, and the ethyl acetate fraction of Example 4 showed a cell proliferation effect of approximately 116.7% at a concentration of 100 μg / ml (Fig. 4). Fig. 4 shows the results of the evaluation of dermal papilla cell proliferation for the Physalis extracts and fractions thereof of Examples 1 to 5.

[0061] [Experimental Example 3: Evaluation of Hair Growth Factor Expression Promotion Effect]

[0062] 1×10 dermal papilla cells 5 Cells were dispensed into a 96-well plate at a concentration of cells / mL and cultured for one day in a 37°C, 5% CO2 cell incubator. The Physalis extract of Example 1 and the ethyl acetate fraction of Example 4, which were used to confirm the efficacy of dermal papilla cell proliferation in Experimental Example 2, were each treated at different concentrations (3, 10, 30, and 100 μg / mL) and reacted for 24 hours in a 37°C, 5% CO2 cell incubator. Subsequently, the cell culture medium was measured using an enzyme-linked immunosorbent assay (ELISA).

[0063] The expression of IGF-1 (Insuline-like growth factor) and VEGF (Vascular endothelial growth factor) in cell culture medium was analyzed using an ELISA kit, and the absorbance was analyzed at 450 nm.

[0064] As a result of the experiment, it was confirmed that both the Physalis extract of Example 1 and the ethyl acetate fraction of Example 4 had an effect of increasing IGF-1 and VEGF expression (Figs. 5 and 6). Figs. 5 and 6 show the results of confirming the expression levels of IGF-1 (Insuline-like growth factor, Fig. 5) and VEGF (Vascular endothelial growth factor, Fig. 6) in the Physalis extract of Example 1 and the fraction of Example 4.

[0066] [Formulation Example 1: Preparation of a hair tonic for preventing hair loss containing Physalis extract]

[0067] Representatively, a hair tonic for preventing hair loss containing the Physalis extract of Example 1 was prepared with the composition of Table 2 below.

[0068] ingredient Formulation Example 1 (weight%) Comparative example 1 ( weight% ) Examples 1 3.0 - Disodium EDTA 0.02 0.02 Panthenol 0.5 0.5 Butylene glycol 10.0 10.0 ethanol 18.0 18.0 menthol 0.03 0.03 Polysorbate 80 1.0 1.0 Imidazolidinyl urea 0.2 0.2 biotin 0.1 0.1 incense Appropriate amount Appropriate amount purified water Remaining amount Remaining amount total 100.0 100.0

Claims

Claim 1 A cosmetic composition for preventing or improving hair loss comprising a 70% (v / v) ethanol extract of Physalis or an ethyl acetate fraction thereof. Claim 2 delete Claim 3 delete Claim 4 delete Claim 5 A cosmetic composition according to claim 1, wherein the cosmetic composition has any one formulation selected from the group consisting of shampoo, rinse, treatment, hair pack, hair gel, ampoule, hair tonic, essence, hair cream, mist, and soap.

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