Exosomal isolation and purification kit

Abstract

This utility model relates to biotechnology, nanobiotechnology, and laboratory research, specifically to means for isolating and purifying exosomes from biological fluids and conditioned cell culture media. The proposed utility model is a kit for isolating and purifying exosomes, including a reagent for precipitating exosomes and a chromatography column that provides additional selective purification of the fraction obtained after exosome precipitation. The kit provides additional removal of associated protein components and lipoproteins, reduces contamination, increases the purity of isolated exosomes, preserves their structural integrity, and improves the reproducibility of their physicochemical properties during repeated isolations. This technical result is achieved by combining exosome precipitation with subsequent purification on a chromatography column without the use of ultracentrifugation. The kit is suitable for the isolation and purification of exosomes from biological fluids and conditioned cell culture media and can be used for scientific research and laboratory analytical purposes.