Method for preparing serum suitable for high-throughput sequencing of chicken mirna

Abstract

The present invention relates to a method for preparing serum suitable for high- throughput sequencing of chicken miRNA. Prepared according to the following method: 1) After collecting fresh blood, immediately place it in a 4°C refrigerated centrifuge at a speed of 800~900 rpm / min for 4~6 min, remove blood cells and other contents, slowly aspirate the supernatant with a nuclease-free pipette tip, transfer to another new nuclease-free tube, strictly avoiding blood cells entering the aspirated supernatant; 2) Take the supernatant obtained in step 1), immediately place it in a 4°C refrigerated centrifuge at a speed of 1800~2000 rpm / min for 8~12 min to remove residual blood cells. Slowly aspirate the supernatant with a nuclease-free pipette tip and transfer it to another new nuclease-free tube; 3) Take the supernatant obtained in step 2), immediately store it in liquid nitrogen.