Method for simultaneously separating six phenolic substances from elm leaves, barks and fruits and application thereof

Abstract

The invention belongs to the technical field of plant component analysis, and in particular relates to a method for simultaneously separating six phenolic substances from elm leaves, barks and fruits and an application thereof. The method comprises the followings: gradient elution separation is carried out by using a solid-liquid separation method, wherein the mobile phase A is as follows: mmol of ammonium acetate and 0.1% formic acid, and the mobile phase B is as follows: methanol. The method for detecting six phenolic substances based on this method has good precision, repeatability and recovery rate. And the analysis can be completed within 10 min.