HETEROCYCLIC AMIDES USEFUL AS PROTEIN MODULATORS

Description

Related Application

[0001] The present application claims priority from US Provisional Application No. 62 / 319358 filed on April 7, 2016, US Provisional Application No. 62 / 461301 filed on February 21, 2017, and US provisional application No. 62 / 461975 filed on February 22, 2017.Field of the Invention

[0002] The present invention relates to a heterocyclic amide that is useful as a modulator of transmembrane protein 173 (TMEM173), which is also known as STING (Stimulator of Interferon Genes)) and methods of making and using the same.Background of the Invention

[0003] Vertebrates are constantly threatened by the invasion of microorganisms and have evolved mechanisms of immune defense to eliminate infective pathogens. In mammals, this immune system comprises two branches; innate immunity and adaptive immunity. The innate immune system is the first line of defense which is initiated by Pattern Recognition Receptors (PRRs) which detect ligands from the pathogens as well as damage associated molecular patterns (Takeuchi O. et al, Cell, 2010: 140, 805-820). A growing number of these receptors have been identified including Toll-like receptors (TLRs), C-type lectin receptors, retinoic acid inducible gene I (RIG-I)-like receptors and NOD-like receptors (NLRs) and also double stranded DNA sensors. Activation of PRRs leads to up-regulation of genes involved in the inflammatory response including type 1 interferons, pro-inflammatory cytokines and chemokines which suppress pathogen replication and facilitate adaptive immunity.

[0004] The adaptor protein STING (Stimulator of Interferon Genes), also known as TMEM 173, MPYS, MITA and ERIS, has been identified as a central signaling molecule in the innate immune response to cytosolic nucleic acids (Ishikawa H and Barber G N, Nature, 2008: 455, 674-678; WO2013 / 1666000). Activation of STING results in up-regulation of IRF3 and NFκB pathways leading to induction of Interferon-β and other cytokines. STING is critical for responses to cytosolic DNA of pathogen or host origin, and of unusual nucleic acids called Cyclic Dinucleotides (CDNs)

[0005] CDNs were first identified as bacterial secondary messengers responsible for controlling numerous responses in the prokaryotic cell. Bacterial CDNs, such as c-di-GMP are symmetrical molecules characterized by two 3',5' phosphodiester linkages.

[0006] Direct activation of STING by bacterial CDNs has recently been confirmed through X-ray crystallography (Burdette D L and Vance R E, Nature Immunology, 2013: 14, 19-26). Bacterial CDNs and their analogues have consequently attracted interest as potential vaccine adjuvants (Libanova R. et al, Microbial Biotechnology 2012: 5, 168-176; WO2007 / 054279, WO2005 / 087238).

[0007] More recently, the response to cytosolic DNA has been elucidated and shown to involve generation, by an enzyme called cyclic GMP-AMP synthase (cGAS, previously known as C6orf150 or MB21D1), of a novel mammalian CDN signaling molecule identified as cGAMP, which then activates STING. Unlike bacterial CDNs, cGAMP is an unsymmetrical molecule characterized by its mixed 2',5' and 3',5' phosphodiester linkages. (Gao P et al, Cell, 2013: 153, 1094-1107). Interaction of cGAMP (II) with STING has also been demonstrated by X-ray crystallography (Cai X et al, Molecular Cell, 2014: 54, 289-296).

[0008] Interferon was first described as a substance which could protect cells from viral infection (Isaacs & Lindemann, J. Virus Interference. Proc. R. Soc. Lon. Ser. B. Biol. Sci. 1957: 147, 258-267). In man, the type I interferons are a family of related proteins encoded by genes on chromosome 9 and encoding at least 13 isoforms of interferon alpha (IFNα) and one isoform of interferon beta (IFNβ). Recombinant IFNα was the first approved biological therapeutic and has become an important therapy in viral infections and in cancer. As well as direct antiviral activity on cells, interferons are known to be potent modulators of the immune response, acting on cells of the immune system.

[0009] Administration of a small molecule compound which could modulate the innate immune response, including the activation or inhibition of type I interferon production and other cytokines, could become an important strategy for the treatment or prevention of human diseases including viral infections and autoimmune disease. This type of immunomodulatory strategy has the potential to identify compounds which may be useful not only in infectious diseases innate immunity but also in cancer (Zitvogel, L., et al., Nature Reviews Immunology, 2015 15(7), p405-414), allergic diseases (Moisan J. et al, Am. J. Physiol. Lung Cell Mol. Physiol., 2006: 290, L987-995), neurodegenerative diseases such as amyotrophic lateral sclerosis and multiple sclerosis (Lemos, H. et al., J. Immunol., 2014: 192(12), 5571-8; Cirulli, E. et al., Science, 2015: 347(6229), 1436-41; Freischmidt, A., et al., Nat. Neurosci., 18(5), 631-6), other inflammatory conditions such as irritable bowel disease (Rakoff-Nahoum S., Cell., 2004, 23, 118(2): 229-41), and as vaccine adjuvants (Persing et al. Trends Microbiol. 2002: 10(10 Suppl), S32-7 and Dubensky et al., Therapeutic Advances in Vaccines, published on line Sept. 5, 2013).

[0010] STING is essential for antimicrobial host defense, including protection against a range of DNA and RNA viruses and bacteria (reviewed in Barber et al. Nat. Rev. Immunol. 2015: 15(2): 87-103, Ma and Damania, Cell Host & Microbe, 2016: 19(2) 150-158). Herpesviridae, Flaviviridae, Coronaviridae, Papillomaviridae, Adenoviridae, Hepadnaviridae, ortho- and paramyxoviridae and rhabdoviridae have evolved mechanisms to inhibit STING mediated Type I interferon production and evade host immune control (Holm et al., Nat Comm. 2016: 7:10680; Ma et al, PNAS 2015: 112(31) E4306-E4315; Wu et al, Cell Host Microbe 2015: 18(3) 333-44; Liu et al, J Virol 2016: 90(20) 9406-19; Chen et al., Protein Cell 2014: 5(5) 369-81; Lau et al, Science 2013: 350(6260) 568-71; Ding et al, J Hepatol 2013: 59(1) 52-8; Nitta et al, Hepatology 2013 57(1) 46-58; Sun et al, PloS One 2012: 7(2) e30802; Aguirre et al, PloS Pathog 2012: 8(10) e1002934; Ishikawa et al, Nature 2009: 461(7265) 788-92). Thus, small molecule activation of STING could be beneficial for treatment of these infectious diseases.

[0011] In contrast, increased and prolonged type I IFN production is associated with a variety of chronic infections, including Mycobacteria (Collins et al, Cell Host Microbe 2015: 17(6) 820-8); Wassermann et al., Cell Host Microbe 2015: 17(6) 799-810; Watson et al., Cell Host Microbe 2015: 17(6) 811-9), Franciscella (Storek et al., J Immunol. 2015: 194(7) 3236-45; Jin et al., J Immunol. 2011: 187(5) 2595-601), Chlamydia (Prantner et al., J Immunol 2010: 184(5) 2551-60; , Plasmodium (Sharma et al., Immunity 2011: 35(2) 194-207. and HIV (Herzner et al., Nat Immunol 2015 16(10) 1025-33; Gao et al., Science 2013: 341(6148) 903-6. Similarly, excess type I interferon production is found among patients with complex forms of autoimmune disease. Genetic evidence in humans and support from studies in animal models support the hypothesis that inhibition of STING results in reduced type I interferon that drives autoimmune disease (Crow YJ, et al., Nat. Genet. 2006; 38(8) 38917-920, Stetson DB, et al., Cell 2008; 134 587-598). Therefore, inhibitors of STING provide a treatment to patients with chronic type I interferon and proinflammatory cytokine production associated with infections or complex autoimmune diseases. Allergic diseases are associated with a Th2-biased immune-response to allergens. Th2 responses are associated with raised levels of IgE, which, via its effects on mast cells, promotes a hypersensitivity to allergens, resulting in the symptoms seen, for example, in allergic rhinitis and asthma. In healthy individuals the immune-response to allergens is more balanced with a mixed Th2 / Th1 and regulatory T cell response. Induction of Type 1 interferons have been shown to result in reduction of Th2-type cytokines in the local environment and promote Th1 / Treg responses. In this context, induction of type 1 interferons by, for example, activation of STING, may offer benefit in treatment of allergic diseases such as asthma and allergic rhinitis (HuberJ.P. et al J Immunol 2010: 185, 813-817).

[0012] Compounds that bind to STING and act as agonist have been shown to induce type 1 interferons and other cytokines on incubation with human PBMCs. Compounds which induce human interferons may be useful in the treatment of various disorders, for example the treatment of allergic diseases and other inflammatory conditions for example allergic rhinitis and asthma, the treatment of infectious diseases, neurodegenerative disease, pre-cancerous syndromes and cancer, and may also be useful as immugenic composition or vaccine adjuvants. Compounds that bind to STING may act as antagonists and could be useful in the treatment, for example of autoimmune diseases. It is envisaged that targeting STING with activation or inhibiting agents may be a promising approach for treating diseases and conditions in which modulation for the type 1 IFN pathway is beneficial, including inflammatory, allergic and autoimmune diseases, infectious diseases, cancer, pre-cancerous syndromes and as immugenic composition or vaccine adjuvants.

[0013] Skin cancers and various skin viral infections involve immune privileged environment and activation of local immune response to the lesions may be a topical therapeutic approach. STING agonists may be used for treating viral warts, superficial skin cancers and premalignant actinic keratoses. By a dual mechanism of action, STING activation (e.g., via microneedle patch delivery or topical formulation) may be used to control HPV directly via antiviral type I interferon production and indirectly by enhancing the adaptive immune response downstream of innate immune activation. STING agonist can activate the innate immune response in the lesion and drive the anti-HPV T-cell response.

[0014] Recent evidence has indicated that spontaneous activation of the STING pathway within tumor-resident dendritic cells leads to type I IFN production and adaptive immune responses against tumors. Furthermore, activation of this pathway in antigen presenting cells (APCs) within the tumor microenvironment drives the subsequent T-cell priming against tumor-associated antigens. Corrales and Gajewski, Clin Cancer Res; 21(21); 4774-9, 2015.

[0015] International Patent Applications WO2014 / 093936, WO2014 / 189805, WO2013 / 185052, U.S.2014 / 0341976, WO 2015 / 077354, PCT / EP2015 / 062281 (WO2015 / 185565) and GB 1501462.4 disclose certain cyclic di-nucleotides and their use in inducing an immune response via activation of STING.

[0016] WO2012 / 083053 describes anti-hepatitis C virus (HCV) compounds of Formula: compositions comprising the same and their use in treating an HCV infection.

[0017] WO2011 / 091446 describes antiviral compounds of Formula: compositions comprising the same and their use in treating a viral infection, for example an HCV infection.

[0018] The compounds of this invention modulate the activity of STING, and accordingly, may provide a beneficial therapeutic impact in treatment of diseases, disorders and / or conditions in which modulation of STING (Stimulator of Interferon Genes) is beneficial, for example for inflammation, allergic and autoimmune diseases, infectious diseases, cancer, pre-cancerous syndromes and as vaccine adjuvants.SUMMARY OF THE INVENTION

[0019] The invention is directed to a compound which is (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide having the structure of or a tautomer thereof, or a pharmaceutically acceptable salt thereof, or a hydrate thereof.

[0020] It is to be understood that the references herein to (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide and salts thereof covers (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide, as the free base, or as salts thereof, for example as pharmaceutically acceptable salts thereof. Thus, in one embodiment, the invention is directed to (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide as the free base. In another embodiment, the invention is directed to (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide and pharmaceutically acceptable salts thereof.

[0021] (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide, tautomers thereof, pharmaceutically acceptable salts thereof and hydrates thereof, are modulators of STING. Accordingly, this invention provides (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide carboxamide or a tautomer thereof, or a pharmaceutically acceptable salt thereof, or a hydrate thereof, for use in therapy. This invention specifically provides for the use of (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof, or a pharmaceutically acceptable salt thereof, or a hydrate thereof, as an active therapeutic substance in the treatment of a STING-mediated disease or disorder, specifically, for use in the treatment of a disease mediated by agonism or antagonism of STING.

[0022] Such STING-mediated diseases or disorders include inflammation, allergic and autoimmune diseases, infectious diseases, cancer, and pre-cancerous syndromes. In addition, modulators of STING may be useful as immugenic composition or vaccine adjuvants.

[0023] The present invention is further directed to a pharmaceutical composition comprising (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide, or a tautomer thereof, or a pharmaceutically acceptable salt thereof, or a hydrate thereof and a pharmaceutically acceptable excipient. Particularly, this invention is directed to a pharmaceutical composition for the treatment of a STING-mediated disease or disorder, where the composition comprises (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide, or a tautomer thereof, or a pharmaceutically acceptable salt thereof, or a hydrate thereof and a pharmaceutically acceptable excipient.Detailed Description of the Application

[0024] According to one aspect of the present invention, this invention relates to a compound which is (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide having the structure of or a tautomer thereof, or a pharmaceutically acceptable salt thereof, or a hydrate thereof.

[0025] Disclosed herein are compounds of Formula (I-N) wherein: q is 0 or 1; r is 0 or 1; s is 0 or 1; wherein q + r + s = 1 or 2; when q is 0, R A1< and R A2< are each independently H, halogen, hydroxy, -O-P(O)(OH) 2 , -O-P(O)(R I< R II< ) 2 , -N(R e< )(R f< ), -CO 2 R f< , -N(R f< )COR b< , -N(R g< )SO 2 (C 1 -C 4 alkyl)-N(R e< )(R f< ), -N(R g< )CO(C 1 -C 4 alkyl)-N(R h< )(R f< ), optionally substituted (C 1 -C 6 alkyl), optionally substituted (C 1 -C 6 alkyl)oxy-, optionally substituted (C 1 -C 6 alkyl)amino-, and optionally substituted (C 1 -C 6 alkyl)(C 1 -C 4 alkyl)amino-, wherein the (C 1 -C 6 alkyl) of said optionally substituted (C 1 -C 6 alkyl), optionally substituted (C 1 -C 6 alkyl)oxy-, optionally substituted (C 1 -C 6 alkyl)amino- and optionally substituted (C 1 -C 6 alkyl)(C 1 -C 4 alkyl)amino- is optionally substituted by 1-4 substituents each independently selected from hydroxy, -O-P(O)(OH) 2 , -O-P(O)(R I< R II< ) 2 , C 1 -C 4 alkoxy-, -N(R e< )(R f< ), -CO 2 (R f< ), -CON(R e< )(R f< ), optionally substituted phenyl, optionally substituted 5-6 membered heterocycloalkyl and optionally substituted 5-6 membered heteroaryl group, wherein said optionally substituted phenyl, 5-6 membered heterocycloalkyl or 5-6 membered heteroaryl is optionally substituted by 1-4 substituents each independently selected from halogen, hydroxy, -O-P(O)(OH) 2 , -O-P(O)(R I< R II< )2, amino, (C 1 -C 6 alkyl)amino-, (C 1 -C 6 alkyl)(C 1 -C 6 alkyl)amino-, -(C 1 -C 6 alkyl)-NH 2 , halo(C 1 -C 6 alkyl), hydroxy-(C 1 -C 4 alkyl)-, -(C 1 -C 4 alkyl)-O-P(O)(OH) 2 , -(C 1 -C 4 alkyl)-O-P(O)(R I< R II< ) 2 , halo(C 1 -C 4 alkoxy)-, C 1 -C 4 alkoxy-, hydroxy-(C 2 -C 4 alkoxy)-, -(C 2 -C 4 alkoxy)-O-P(O)(OH) 2 , -(C 2 -C 4 alkoxy)-O-P(O)(R I< R II< )2, -C 1 -C 4 alkyl-(C 1 -C 4 alkoxy) and C 1 -C 4 alkoxy-(C 1 -C 4 alkoxy)-; when r is 0, R B1< and R B2< are each independently H, optionally substituted C 1 -C 6 alkyl, halo(C 1 -C 6 alkyl), optionally substituted C 2 -C 6 alkenyl, optionally substituted C 2 -C 6 alkynyl, optionally substituted C 3 -C 6 cycloalkyl, optionally substituted 4-6 membered heterocycloalkyl, optionally substituted phenyl, optionally substituted 5-6 membered heteroaryl, or optionally substituted 9-10 membered heteroaryl, wherein said optionally substituted C 1 -C 6 alkyl, optionally substituted C 2 -C 6 alkenyl, optionally substituted C 2 -C 6 alkynyl, optionally substituted C 3 -C 6 cycloalkyl, optionally substituted 4-6 membered heterocycloalkyl, optionally substituted phenyl, optionally substituted 5-6 membered heteroaryl, or optionally substituted 9-10 membered heteroaryl is optionally substituted by 1-4 substituents each independently selected from halogen, nitro, -R c< , -OH, -O-P(O)(OH) 2 , -O-P(O)(R I< R II< )2, -OR c< , -NHz, -NR c< R c< , -NR c< R d< , -OCOR c< , -CO 2 H, -CO 2 R c< , -SOR c< , -SO 2 R c< , -CONHz, -CONR c< R d< , -SO 2 NH 2 , -SO 2 N R c< R d< , -OCONH 2 , -OCONR c< R d< , -NR d< COR c< , -NR d< SOR c< , -NR d< CO 2 R c< , and -NR d< SO 2 R c< ; when s is 0, R C1< is H, halogen, or C 1 -C 4 alkyl and R C2< is optionally substituted C 1 -C 4 alkyl, wherein said optionally substituted C 1 -C 4 alkyl group is optionally substituted by a substituent selected from -OR c< , -NR c< R d< , -CO 2 R c< , -CONR c< R d< , -SO 2 NR c< R d< , and -OCONR c< R d< ; when q is 1, R A1< and R A2< are each independently -CH 2 -, -NR e< -, or -O-, and A, taken together with R A1< and R A2< , forms a linking group, wherein A is -halo(C 1 -C 12 alkyl)-, optionally substituted -C 1 -C 12 alkyl-, optionally substituted -C 2 -C 12 alkenyl-, optionally substituted -C 2 -C 12 alkynyl-, optionally substituted -C 1 -C 6 alkyl-O-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-NR a< -C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-(C 3 -C 6 cycioalkyl)-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-phenyl-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-(4-6 membered heterocycloalkyl)-C 1 -C 6 alkyl-, or optionally substituted -C 1 -C 6 alkyl-(5-6 membered heteroaryl)-C 1 -C 6 alkyl-, wherein the alkyl moiety of said optionally substituted -C 1 -C 12 alkyl-, optionally substituted -C 2 -C 12 alkenyl-, optionally substituted -C 2 -C 12 alkynyl-, optionally substituted -C 1 -C 6 alkyl-O-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-NR a< -C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-(C 3 -C 6 cycioalkyl)-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-phenyl-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-(4-6 membered heterocycloalkyl)-C 1 -C 6 alkyl-, or optionally substituted -C 1 -C 6 alkyl-(5-6 membered heteroaryl)-C 1 -C 6 alkyl- is optionally substituted by 1-4 substituents each independently selected from halogen, halo(C 1 -C 4 alkyl), -OH, -O-P(O)(OH) 2 , -O-P(O)(R I< R II< ) 2 , -OR c< , -NH 2 , -NR c< R d< , -OCOR c< , -CO 2 H, -CO 2 R c< , -SOR c< , -SO 2 R c< , -CONH 2 , -CONR c< R d< , -SO 2 NH 2 , -SO 2 NR c< R d< , -OCONH 2 , -OCONR c< R d< , -NR d< COR c< , -NR d< SOR c< , -NR d< CO 2 R c< , and -NR d< SO 2 R c< , and the C 3 -C 6 cycloalkyl, phenyl, 4-6 membered heterocycloalkyl, or 5-6 membered heteroaryl moiety of said optionally substituted -C 1 -C 6 alkyl-(C 3 -C 6 cycioalkyl)-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-phenyl-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-(4-6 membered heterocycloalkyl)-C 1 -C 6 alkyl-, or optionally substituted -C 1 -C 6 alkyl-(5-6 membered heteroaryl)-C 1 -C 6 alkyl- is optionally substituted by 1-4 substituents each independently selected from halogen, hydroxy, -O-P(O)(OH) 2 , -O-P(O)(R I< R II< )2, amino, (C 1 -C 4 alkyl)amino-, (C 1 -C 4 alkyl)(C 1 -C 4 alkyl)amino-, C 1 -C 4 alkyl, halo(C 1 -C 4 alkyl), halo(C 1 -C 4 alkoxy)-, C 1 -C 4 alkoxy-, hydroxy-(C 1 -C 4 alkoxy)-, -(C 1 -C 4 alkoxyl)-O-P(O)(OH) 2 , -(C 1 -C 4 alkoxyl)-O-P(O)(R I< R II< ) 2 and C 1 -C 4 alkoxy-(C 1 -C 4 alkoxy)-; when r is 1, R B1< and R B2< are each independently -CH 2 -, and B, taken together with R B1< and R B2< , forms a linking group, wherein B is a bond or B is -halo(C 1 -C 10 alkyl)-, optionally substituted -C 1 -C 10 alkyl-, optionally substituted -C 2 -C 10 alkenyl-, optionally substituted -C 2 -C 10 alkynyl-, optionally substituted -C 1 -C 6 alkyl-O-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-NR a< -C 1 -C 6 alkyl-, optionally substituted C 3 -C 6 cycloalkyl, optionally substituted phenyl, optionally substituted 4-6 membered heterocycloalkyl, optionally substituted 5-6 membered heteroaryl, optionally substituted -C 1 -C 4 alkyl-(C 3 -C 6 cycloalkyl)-C 3 -C 4 alkyl-, optionally substituted -C 1 -C 4 alkyl-phenyl-C 1 -C 4 alkyl-, optionally substituted -C 1 -C 4 alkyl-(4-6 membered heterocycloalkyl)-C 1 -C 4 alkyl-, or optionally substituted -C 1 -C 4 alkyl-(5-6 membered heteroaryl)-C 1 -C 4 alkyl-, wherein the alkyl moiety of said optionally substituted -C 1 -C 10 alkyl-, optionally substituted -C 2 -C 10 alkenyl-, optionally substituted -C 2 -C 10 alkynyl-, optionally substituted -C 1 -C 6 alkyl-O-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-NR a< -C 1 -C 6 alkyl-, optionally substituted -C 1 -C 4 alkyl-(C 3 -C 6 cycloalkyl)-C 3 -C 4 alkyl-, optionally substituted -C 1 -C 4 alkyl-phenyl-C 1 -C 4 alkyl-, optionally substituted -C 1 -C 4 alkyl-(4-6 membered heterocycloalkyl)-C 1 -C 4 alkyl-, or optionally substituted -C 1 -C 4 alkyl-(5-6 membered heteroaryl-C 1 -C 4 alkyl)- is optionally substituted by 1 or 2 substituents each independently selected from halogen, halo(C 1 -C 4 alkyl), -OH, -O-P(O)(OH) 2 , -O-P(O)(R I< R II< ) 2 , -OR c< , -NH 2 , -NR c< R d< , -OCOR c< , -CO 2 H, -CO 2 R c< , -SOR c< , -SO 2 R c< , -CONH 2 , -CONR c< R d< , -SO 2 NH 2 , -SO 2 NR c< R d< , -OCONH 2 , -OCONR c< R d< , -NR d< COR c< , -NR d< SOR c< , -NR d< CO 2 R c< , and -NR d< SO 2 R c< , and the C 3 -C 6 cycloalkyl, phenyl, 4-6 membered heterocycloalkyl, or 5-6 membered heteroaryl moiety of said optionally substituted C 3 -C 6 cycloalkyl, optionally substituted phenyl, optionally substituted 4-6 membered heterocycloalkyl, optionally substituted 5-6 membered heteroaryl, optionally substituted -C 1 -C 4 alkyl-(C 3 -C 6 cycloalkyl)-C 3 -C 4 alkyl-, optionally substituted -C 1 -C 4 alkyl-phenyl-C 1 -C 4 alkyl-, optionally substituted -C 1 -C 4 alkyl-(4-6 membered heterocycloalkyl)-C 1 -C 4 alkyl-, or optionally substituted -C 1 -C 4 alkyl-(5-6 membered heteroaryl)-C 1 -C 4 alkyl- is optionally substituted by 1-4 substituents each independently selected from halogen, hydroxy, -O-P(O)(OH) 2 , -O-P(O)(R I< R II< )2, amino, (C 1 -C 4 alkyl)amino-, (C 1 -C 4 alkyl)(C 1 -C 4 alkyl)amino-, C 1 -C 4 alkyl, halo(C 1 -C 4 alkyl), halo(C 1 -C 4 alkoxy)-, C 1 -C 4 alkoxy-, hydroxy-(C 2 -C 4 alkoxy)-, -(C 2 -C 4 alkoxy) O-P(O)(OH) 2 , -(C 2 -C 4 alkoxy)-O-P(O)(R I< R II< ) 2 , and C 1 -C 4 alkoxy-(C 1 -C 4 alkoxy)-; when s is 1, R C1< and R C2< are each independently -CH 2 -, and C, taken together with R C1< and R C2< , forms a linking group, wherein C is -halo(C 1 -C 12 alkyl)-, optionally substituted -C 1 -C 12 alkyl-, optionally substituted -C 2 -C 12 alkenyl-, optionally substituted -C 2 -C 12 alkynyl-, optionally substituted -C 1 -C 6 alkyl-O-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-NR a< C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-(C 3 -C 6 cycloalkyl)-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-phenyl-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-(4-6 membered heterocycloalkyl)-C 1 -C 6 alkyl-, or optionally substituted -C 1 -C 6 alkyl-(5-6 membered heteroaryl)-C 1 -C 6 alkyl-, wherein the alkyl moiety of said optionally substituted -C 1 -C 12 alkyl-, optionally substituted -C 2 -C 12 alkenyl-, optionally substituted -C 2 -C 12 alkynyl-, optionally substituted -C 1 -C 6 alkyl-O-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-NR a< -C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-(C 3 -C 6 cycloalkyl)-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-phenyl-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-(4-6 membered heterocycloalkyl)-C 1 -C 6 alkyl-, or optionally substituted -C 1 -C 6 alkyl-(5-6 membered heteroaryl)-C 1 -C 6 alkyl- is optionally substituted by 1 or 2 substituents each independently selected from halogen, halo(C 1 -C 4 alkyl), -OH, -O-P(O)(OH) 2 , -O-P(O)(R I< R II< ) 2 , -OR c< , -NH 2 , -NR c< R d< , -OCOR c< , -CO 2 H, -CO 2 R c< , -SOR c< , -SO 2 R c< , -CONH 2 , -CONR c< R d< , -SO 2 NH 2 , -SO 2 NR c< R d< , -OCONH 2 , -OCONR c< R d< , -NR d< COR c< , -NR d< S OR c< , -NR d< CO 2 R c< , and -NR d< SO 2 R c< , and the C 3 -C 6 cycloalkyl, phenyl, 4-6 membered heterocycloalkyl, or 5-6 membered heteroaryl moiety of said optionally substituted -C 1 -C 6 alkyl-(C 3 -C 6 cycloalkyl)-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-phenyl-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-(4-6 membered heterocycloalkyl)-C 1 -C 6 alkyl-, or optionally substituted -C 1 -C 6 alkyl-(5-6 membered heteroaryl)-C 1 -C 6 alkyl- is optionally substituted by 1-4 substituents each independently selected from halogen, hydroxy, -O-P(O)(OH) 2 , -O-P(O)(R I< R II< )2, amino, (C 1 -C 4 alkyl)amino-, (C 1 -C 4 alkyl)(C 1 -C 4 alkyl)amino-, C 1 -C 4 alkyl, halo(C 1 -C 4 alkyl), halo(C 1 -C 4 alkoxy)-, C 1 -C 4 alkoxy-, hydroxy-(C 2 -C 4 alkoxy)-, -(C 2 -C 4 alkoxy)-O-P(O)(OH) 2 , -(C 2 -C 4 alkoxy)-O-P(O)(RIR II< ) 2 , and C 1 -C 4 alkoxy-(C 1 -C 4 alkoxy)-; R 3< and R 5< are each independently -CON(R d< )(R f< ), or one of R 3< and R 5< is -CON(R d< )(R f< ), and the other of R 3< and R 5< is H, COOH or -CO 2 (R c< ); R 4< and R 6< are each independently selected from H, halogen, halo(C 1 -C 6 alkyl), halo(C 1 -C 6 alkoxy)-, hydroxy, -O-P(O)(OH) 2 , -O-P(O)(R I< R II< )2, -NH 2 , -NR c< R c< , -NR c< R d< , -COR c< , -CO 2 R c< , -N(R d< )COR c< , -N(R d< )SO 2 R c< , -N(R g< )SO 2 (C 1 -C 2 alkyl)-N(R h< )(R f< ), -N(R g< )CO(C 1 -C 2 alkyl)-N(R h< )(R f< ), optionally substituted (C 1 -C 6 alkyl), optionally substituted (C 1 -C 6 alkyl)oxy-, optionally substituted (C 1 -C 6 alkyl)amino-, and optionally substituted (C 1 -C 6 alkyl)(C 1 -C 4 alkyl)amino-, wherein the (C 1 -C 6 alkyl) of said optionally substituted (C 1 -C 6 alkyl), optionally substituted (C 1 -C 6 alkyl)oxy-, optionally substituted (C 1 -C 6 alkyl)amino- and optionally substituted (C 1 -C 6 alkyl)(C 1 -C 4 alkyl)amino- is optionally substituted by 1-4 substituents each independently selected from -OH, -O-P(O)(OH) 2 , -OP(O)(R I< R II< )2,-OR c< , -NH 2 , -NR c< R c< , -NR c< R d< , -CO 2 H, -CO 2 R c< , -OCOR c< , -COzH, -CO 2 R c< , -SOR c< , -SO 2 R c< , -CONHz, -CONR c< R d< , -SO 2 NH 2 , -SO 2 NR c< R d< , -OCONHz, -OCONR c< R d< , -N R d< COR c< , -NR d< SOR c< , -NR d< CO 2 R c< , -NR d< SO 2 R c< , optionally substituted phenyl, optionally substituted 5-6 membered heterocycloalkyl and optionally substituted 5-6 membered heteroaryl group, wherein said optionally substituted phenyl, 5-6 membered heterocycloalkyl or 5-6 membered heteroaryl is optionally substituted by 1-4 substituents each independently selected from halogen, hydroxy, -O-P(O)(OH) 2 , -O-P(O)(R I< R II< )2, amino, (C 1 -C 4 alkyl)amino-, (C 1 -C 4 alkyl)(C 1 -C 4 alkyl)amino-, C 1 -C 4 alkyl, halo(C 1 -C 4 alkyl), hydroxy-(C 1 -C 4 alkyl)-, -(C 1 -C 4 alkyl)-O-P(O)(OH) 2 , -(C 1 -C 4 alkyl)-O-P(O)(R I< R II< ) 2 , halo(C 1 -C 4 alkoxy)-, C 1 -C 4 alkoxy-, hydroxy-(C 2 -C 4 alkoxy)-, -(C 2 -C 4 alkoxy)-O-P(O)(OH) 2 , -(C 2 -C 4 alkoxy)-O-P(O)(R I< R II< ) 2 , C 1 -C 4 alkoxy-(C 1 -C 4 alkoxy)-, -COR d< , -CON(R d< )(R f< ), and -CO 2 R d< ; R 14< is optionally substituted C 1 -C 4 alkyl, wherein said optionally substituted C 1 -C 4 alkyl is optionally substituted by a substituent selected from -OR c< , -NR c< R d< , -CO 2 R c< , -CONR c< R d< , -SO 2 NR c< R d< , and -OCONR c< R d< ; R 16< is H, halogen, or C 1 -C 4 alkyl; R 15< and R 17< are each independently H, cyclopropyl, or C 1 -C 4 alkyl; R a< is H, -R c< , -COR c< , -CO 2 H, -CO 2 R c< , -SOR c< , -SO 2 R c< , -CONH 2 , -CONR c< R d< , -SO 2 NH 2 , or -SO 2 NR c< R d< ; each R b< is independently C 1 -C 4 alkyl, halo(C 1 -C 4 alkyl), -(C 1 -C 4 alkyl)-OH, -(C 1 -C 4 alkyl)-O-P(O)(OH) 2 , -(C 1 -C 4 alkyl)-O-P(O)(R I< R II< ) 2 , -(C 1 -C 4 alkyl)-O-(C 1 -C 4 alkyl), -(C 1 -C 4 alkyl)-N(R e< )(R f< ), -(C 1 -C 4 alkyl)-O-CO(C 1 -C 4 alkyl), or -(C 1 -C 4 alkyl)-CO-O-(C 1 -C 4 alkyl); each R c< is independently C 1 -C 4 alkyl, halo(C 1 -C 4 alkyl), -(C 1 -C 4 alkyl)-OH, -(C 1 -C 4 alkyl)-O-P(O)(OH) 2 , -(C 1 -C 4 alkyl)-O-P(O)(R I< R II< ) 2 ,-(C 1 -C 4 alkyl)-O-(C 1 -C 4 alkyl), -(C 1 -C 4 alkyl)-N(R e< )(R f< ), -(C 1 -C 4 alkyl)-O-CO(C 1 -C 4 alkyl), -(C 1 -C 4 alkyl)-CO-O-(C 1 -C 4 alkyl), optionally substituted C 3 -C 6 cycloalkyl, optionally substituted phenyl, optionally substituted 4-6 membered heterocycloalkyl, optionally substituted 5-6 membered heteroaryl, optionally substituted 9-10 membered heteroaryl, optionally substituted -C 1 -C 4 alkyl-C 3 -C 6 cycloalkyl, optionally substituted -C 1 -C 4 alkyl-phenyl, optionally substituted -C 1 -C 4 alkyl-4-6 membered heterocycloalkyl, optionally substituted -C 1 -C 4 alkyl-5-6 membered heteroaryl, or optionally substituted -C 1 -C 4 alkyl-9-10 membered heteroaryl, wherein the C 3 -C 6 cycloalkyl, phenyl, 4-6 membered heterocycloalkyl, 5-6 membered heteroaryl or 9-10 membered heteroaryl moiety of said optionally substituted C 3 -C 6 cycloalkyl, optionally substituted phenyl, optionally substituted 4-6 membered heterocycloalkyl, optionally substituted 5-6 membered heteroaryl, optionally substituted 9-10 membered heteroaryl optionally substituted -C 1 -C 4 alkyl-C 3 -C 6 cycloalkyl, optionally substituted -C 1 -C 4 alkyl-phenyl, optionally substituted -C 1 -C 4 alkyl-4-6 membered heterocycloalkyl, optionally substituted -C 1 -C 4 alkyl-5-6 membered heteroaryl, or optionally substituted -C 1 -C 4 alkyl-9-10 membered heteroaryl is optionally substituted by 1-4 substituents each independently selected from halogen, hydroxy, -O-P(O)(OH) 2 , -O-P(O)(R I< R II< )2, amino, -(C 1 -C 4 alkyl)NH 2 , (C 1 -C 4 alkyl)amino-, (C 1 -C 4 alkyl)(C 1 -C 4 alkyl)amino-, C 1 -C 4 alkyl, halo(C 1 -C 4 alkyl), halo(C 1 -C 4 alkoxy)-, C 1 -C 4 alkoxy-, hydroxy-(C 2 -C 4 alkoxy)-, -(C 2 -C 4 alkoxy)-O-P(O)(OH) 2 , -(C 2 -C 4 alkoxy)-O-P(O)(R I< R II< ) 2 , C 1 -C 4 alkoxy-(C 1 -C 4 alkoxy)-, -COR d< , -CON(R d< )(R f< ), and -CO 2 R d< ; each R d< is independently H or C 1 -C 4 alkyl; each R e< is independently H, (C 1 -C 4 alkyl), -CO(C 1 -C 4 alkyl), -OCO(C 1 -C 4 alkyl), -CO 2 (C 1 -C 4 alkyl), -(C 1 -C 4 alkyl)NH 2 , -(C 1 -C 4 alkyl) C 1 -C 4 alkoxy, -CO-(optionally substituted 5-6 membered heterocycloalkyl), -CO(C 1 -C 4 alkyl)-(optionally substituted 5-6 membered heterocycloalkyl), -CO(optionally substituted 5-6 membered heteroaryl), -CO(C 1 -C 4 alkyl)-(optionally substituted 5-6 membered heteroaryl), wherein the optionally substituted 5-6 membered heterocycloalkyl or optionally substituted 5-6 membered heteroaryl is optionally substituted 1-4 substituents each independently selected from halogen, hydroxy, -O-P(O)(OH) 2 , -O-P(O)(R I< R II< )2, amino, (C 1 -C 4 alkyl)amino-, (C 1 -C 4 alkyl)(C 1 -C 4 alkyl)amino-, C 1 -C 4 alkyl, halo(C 1 -C 4 alkyl), halo(C 1 -C 4 alkoxy)-, C 1 -C 4 alkoxy-, hydroxy-(C 2 -C 4 alkoxy)-, -(C 2 -C 4 alkoxy) O-P(O)(OH) 2 , -(C 2 -C 4 alkoxy)-O-P(O)(R I< R II< ) 2 , C 1 -C 4 alkoxy-(C 1 -C 4 alkoxy)-, -COR d< , -CON(R d< )(R f< ), and -CO 2 R d< ; each R f< is independently H or (C 1 -C 4 alkyl); R g< and R h< are each independently H or (C 1 -C 4 alkyl) or R g< and R h< , taken together with the atom or atoms through which they are connected, form a 5-6 membered ring; and each occurrence of R I< and R II< are independently (C 1 -C 6 alkyl)1oxy-; or a tautomer thereof; or a salt, particularly a pharmaceutically acceptable salt, thereof.

[0026] Also disclosed herein are compounds according to Formula (I-P): wherein: q is 0 or 1; r is 0 or 1; s is 0 or 1; wherein q + r + s = 1 or 2; when q is 0, R A1< and R A2< are each independently H, halogen, hydroxy, -O-P(O)(OH) 2 , -O-P(O)(R I< R II< ) 2 , -N(R e< )(R f< ), -CO 2 R f< , -N(R f< )COR b< , -N(R g< )SO 2 (C 1 -C 4 alkyl)-N(R e< )(R f< ), -N(R g< )CO(C 1 -C 4 alkyl)-N(R h< )(R f< ), optionally substituted (C 1 -C 6 alkyl), optionally substituted (C 1 -C 6 alkyl)oxy-, optionally substituted (C 1 -C 6 alkyl)amino-, and optionally substituted (C 1 -C 6 alkyl)(C 1 -C 4 alkyl)amino-, wherein the (C 1 -C 6 alkyl) of said optionally substituted (C 1 -C 6 alkyl), optionally substituted (C 1 -C 6 alkyl)oxy-, optionally substituted (C 1 -C 6 alkyl)amino- and optionally substituted (C 1 -C 6 alkyl)(C 1 -C 4 alkyl)amino- is optionally substituted by 1-4 substituents each independently selected from hydroxy, -O-P(O)(OH) 2 , -O-P(O)(R I< R II< ) 2 , C 1 -C 4 alkoxy-, -N(R e< )(R f< ), -CO 2 (R f< ), -CON(R e< )(R f< ), optionally substituted phenyl, optionally substituted 5-6 membered heterocycloalkyl and optionally substituted 5-6 membered heteroaryl group, wherein said optionally substituted phenyl, 5-6 membered heterocycloalkyl or 5-6 membered heteroaryl is optionally substituted by 1-4 substituents each independently selected from halogen, hydroxy, -O-P(O)(OH) 2 , -O-P(O)(R I< R II< )2, amino, (C 1 -C 6 alkyl)amino-, (C 1 -C 6 alkyl)(C 1 -C 6 alkyl)amino-,-(C 1 -C 6 alkyl)-NH 2 , halo(C 1 -C 6 alkyl), hydroxy-(C 1 -C 4 alkyl)-, -(C 1 -C 4 alkyl)-O-P(O)(OH) 2 , -(C 1 -C 4 alkyl)-O-P(O)(R I< R II< ) 2 , halo(C 1 -C 4 alkoxy)-, C 1 -C 4 alkoxy-, hydroxy-(C 2 -C 4 alkoxy)-, -(C 2 -C 4 alkoxy)-O-P(O)(OH) 2 , -(C 2 -C 4 alkoxy)-O-P(O)(R I< R II< ) 2 , or C 1 -C 4 alkoxy-(C 1 -C 4 alkoxy)-; when r is 0, R B1< and R B2< are each independently H, optionally substituted C 1 -C 6 alkyl, halo(C 1 -C 6 alkyl), optionally substituted C 2 -C 6 alkenyl, optionally substituted C 2 -C 6 alkynyl, optionally substituted C 3 -C 6 cycloalkyl, optionally substituted 4-6 membered heterocycloalkyl, optionally substituted phenyl, optionally substituted 5-6 membered heteroaryl, or optionally substituted 9-10 membered heteroaryl, wherein said optionally substituted C 1 -C 6 alkyl, optionally substituted C 2 -C 6 alkenyl, optionally substituted C 2 -C 6 alkynyl, optionally substituted C 3 -C 6 cycloalkyl, optionally substituted 4-6 membered heterocycloalkyl, optionally substituted phenyl, optionally substituted 5-6 membered heteroaryl, or optionally substituted 9-10 membered heteroaryl is optionally substituted by 1-4 substituents each independently selected from halogen, nitro, -R c< , -OH, -O-P(O)(OH) 2 , -O-P(O)(R I< R II< )2, -OR c< , -NH 2 , -NR c< R c< , -NR c< R d< , -OCOR c< , -CO 2 H, -CO 2 R c< , -SOR c< , -SO 2 R c< , -CONH 2 , -CONR c< R d< , -SO 2 NH 2 , -SO 2 N R c< R d< , -OCONH 2 , -OCONR c< R d< , -NR d< COR c< , -NR d< SOR c< , -NR d< CO 2 R c< , and -NR d< SO 2 R c< ; when s is 0, R C1< is H, halogen, or C 1 -C 4 alkyl and R C2< is optionally substituted C 1 -C 4 alkyl, wherein said optionally substituted C 1 -C 4 alkyl group is optionally substituted by a substituent selected from -OR c< , -NR c< R d< , -CO 2 R c< , -CONR c< R d< , -SO 2 NR c< R d< , and -OCONR c< R d< ; when q is 1, R A1< and R A2< are each independently -CH 2 -, -NR e-< , or -O-, and A, taken together with R A1< and R A2< , forms a linking group, wherein A is -halo(C 1 -C 12 alkyl)-, optionally substituted -C 1 -C 12 alkyl-, optionally substituted -C 2 -C 12 alkenyl-, optionally substituted -C 2 -C 12 alkynyl-, optionally substituted -C 1 -C 6 alkyl-O-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-NR a< -C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-(C 3 -C 6 cycloalkyl)-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-phenyl-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-(4-6 membered heterocycloalkyl)-C 1 -C 6 alkyl-, or optionally substituted -C 1 -C 6 alkyl-(5-6 membered heteroaryl)-C 1 -C 6 alkyl-, wherein the alkyl moiety of said optionally substituted -C 1 -C 12 alkyl-, optionally substituted -C 2 -C 12 alkenyl-, optionally substituted -C 2 -C 12 alkynyl-, optionally substituted -C 1 -C 6 alkyl-O-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-NR a< -C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-(C 3 -C 6 cycloalkyl)-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-phenyl-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-(4-6 membered heterocycloalkyl)-C 1 -C 6 alkyl-, or optionally substituted -C 1 -C 6 alkyl-(5-6 membered heteroaryl)-C 1 -C 6 alkyl- is optionally substituted by 1-4 substituents each independently selected from halogen, halo(C 1 -C 4 alkyl), -OH, -O-P(O)(OH) 2 , -O-P(O)(R I< R II< ) 2 , -OR c< , -NH 2 , -NR c< R d< , -OCOR c< , -CO 2 H, -CO 2 R c< , -SOR c< , -SO 2 R c< , -CONHz, -CONR c< R d< , -SO 2 NH 2 , -SO 2 NR c< R d< , -OCONH 2 , -OCONR c< R d< , -NR d< COR c< , -NR d< SOR c< , -NR d< CO 2 R c< , and -NR d< SO 2 R c< , and the C 3 -C 6 cycloalkyl, phenyl, 4-6 membered heterocycloalkyl, or 5-6 membered heteroaryl moiety of said optionally substituted -C 1 -C 6 alkyl-(C 3 -C 6 cycloalkyl)-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-phenyl-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-(4-6 membered heterocycloalkyl)-C 1 -C 6 alkyl-, or optionally substituted -C 1 -C 6 alkyl-(5-6 membered heteroaryl)-C 1 -C 6 alkyl- is optionally substituted by 1-4 substituents each independently selected from halogen, hydroxy, -O-P(O)(OH) 2 , -O-P(O)(R I< R II< )2, amino, (C 1 -C 4 alkyl)amino-, (C 1 -C 4 alkyl)(C 1 -C 4 alkyl)amino-, C 1 -C 4 alkyl, halo(C 1 -C 4 alkyl), halo(C 1 -C 4 alkoxy)-, C 1 -C 4 alkoxy-, hydroxy-(C 1 -C 4 alkoxy)-, -(C 1 -C 4 alkoxyl)-O-P(O)(OH) 2 , -(C 1 -C 4 alkoxyl)-O-P(O)(R I< R II< ) 2 and C 1 -C 4 alkoxy-(C 1 -C 4 alkoxy)-; when r is 1, R B1< and R B2< are each independently -CH 2 -, and B, taken together with R B1< and R B2< , forms a linking group, wherein B is a bond or B is -halo(C 1 -C 10 alkyl)-, optionally substituted -C 1 -C 10 alkyl-, optionally substituted -C 2 -C 10 alkenyl-, optionally substituted -C 2 -C 10 alkynyl-, optionally substituted -C 1 -C 6 alkyl-O-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-NR a< -C 1 -C 6 alkyl-, optionally substituted C 3 -C 6 cycloalkyl, optionally substituted phenyl, optionally substituted 4-6 membered heterocycloalkyl, optionally substituted 5-6 membered heteroaryl, optionally substituted -C 1 -C 4 alkyl-(C 3 -C 6 cycloalkyl)-C 3 -C 4 alkyl-, optionally substituted -C 1 -C 4 alkyl-phenyl-C 1 -C 4 alkyl-, optionally substituted -C 1 -C 4 alkyl-(4-6 membered heterocycloalkyl)-C 1 -C 4 alkyl-, or optionally substituted -C 1 -C 4 alkyl-(5-6 membered heteroaryl)-C 1 -C 4 alkyl-, wherein the alkyl moiety of said optionally substituted -C 1 -C 10 alkyl-, optionally substituted -C 2 -C 10 alkenyl-, optionally substituted -C 2 -C 10 alkynyl-, optionally substituted -C 1 -C 6 alkyl-O-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-NR a< -C 1 -C 6 alkyl-, optionally substituted -C 1 -C 4 alkyl-(C 3 -C 6 cycloalkyl)-C 3 -C 4 alkyl-, optionally substituted -C 1 -C 4 alkyl-phenyl-C 1 -C 4 alkyl-, optionally substituted -C 1 -C 4 alkyl-(4-6 membered heterocycloalkyl)-C 1 -C 4 alkyl-, or optionally substituted -C 1 -C 4 alkyl-(5-6 membered heteroaryl-C 1 -C 4 alkyl)- is optionally substituted by 1 or 2 substituents each independently selected from halogen, halo(C 1 -C 4 alkyl), -OH, -O-P(O)(OH) 2 , -O-P(O)(R I< R II< )2, -OR c< , -NH 2 , -NR c< R d< , -OCOR c< , -CO 2 H, -CO 2 R c< , -SOR c< , -SO 2 R c< , -CONH 2 , -CONR c< R d< , -SO 2 NH 2 , -SO 2 NR c< R d< , -OCONHz, -OCONR c< R d< , -NR d< COR c< , -NR d< SOR c< , -NR d< CO 2 R c< , and -NR d< SO 2 R c< , and the C 3 -C 6 cycloalkyl, phenyl, 4-6 membered heterocycloalkyl, or 5-6 membered heteroaryl moiety of said optionally substituted C 3 -C 6 cycloalkyl, optionally substituted phenyl, optionally substituted 4-6 membered heterocycloalkyl, optionally substituted 5-6 membered heteroaryl, optionally substituted -C 1 -C 4 alkyl-(C 3 -C 6 cycloalkyl)-C 3 -C 4 alkyl-, optionally substituted -C 1 -C 4 alkyl-phenyl-C 1 -C 4 alkyl-, optionally substituted -C 1 -C 4 alkyl-(4-6 membered heterocycloalkyl)-C 1 -C 4 alkyl-, or optionally substituted -C 1 -C 4 alkyl-(5-6 membered heteroaryl)-C 1 -C 4 alkyl- is optionally substituted by 1-4 substituents each independently selected from halogen, hydroxy, -O-P(O)(OH) 2 , -O-P(O)(R I< R II< )2, amino, (C 1 -C 4 alkyl)amino-, (C 1 -C 4 alkyl)(C 1 -C 4 alkyl)amino-, C 1 -C 4 alkyl, halo(C 1 -C 4 alkyl), halo(C 1 -C 4 alkoxy)-, C 1 -C 4 alkoxy-, hydroxy-(C 2 -C 4 alkoxy)-, -(C 2 -C 4 alkoxy) O-P(O)(OH) 2 , -(C 2 -C 4 alkoxy)-O-P(O)(R I< R II< ) 2 , and C 1 -C 4 alkoxy-(C 1 -C 4 alkoxy)-; when s is 1, R C1< and R C2< are each independently -CH 2 -, and C, taken together with R C1< and R C2< , forms a linking group, wherein C is -halo(C 1 -C 12 alkyl)-, optionally substituted -C 1 -C 12 alkyl-, optionally substituted -C 2 -C 12 alkenyl-, optionally substituted -C 2 -C 12 alkynyl-, optionally substituted -C 1 -C 6 alkyl-O-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-NR a< -C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-(C 3 -C 6 cycloalkyl)-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-phenyl-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-(4-6 membered heterocycloalkyl)-C 1 -C 6 alkyl-, or optionally substituted -C 1 -C 6 alkyl-(5-6 membered heteroaryl)-C 1 -C 6 alkyl-, wherein the alkyl moiety of said optionally substituted -C 1 -C 12 alkyl-, optionally substituted -C 2 -C 12 alkenyl-, optionally substituted -C 2 -C 12 alkynyl-, optionally substituted -C 1 -C 6 alkyl-O-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-NR a< -C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-(C 3 -C 6 cycloalkyl)-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-phenyl-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-(4-6 membered heterocycloalkyl)-C 1 -C 6 alkyl-, or optionally substituted -C 1 -C 6 alkyl-(5-6 membered heteroaryl)-C 1 -C 6 alkyl- is optionally substituted by 1 or 2 substituents each independently selected from halogen, halo(C 1 -C 4 alkyl), -OH, -O-P(O)(OH) 2 , -O-P(O)(R I< R II< ) 2 , -OR c< , -NH 2 , -NR c< R d< , -OCOR c< , -CO 2 H, -CO 2 R c< , -SOR c< , -SO 2 R c< , -CONHz, -CONR c< R d< , -SO 2 NH 2 , -SO 2 NR c< R d< , -OCONH 2 , -OCONR c< R d< , -NR d< COR c< , -NR d< SOR c< , -NR d< CO 2 R c< , and -NR d< SO 2 R c< , and the C 3 -C 6 cycloalkyl, phenyl, 4-6 membered heterocycloalkyl, or 5-6 membered heteroaryl moiety of said optionally substituted -C 1 -C 6 alkyl-(C 3 -C 6 cycloalkyl)-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-phenyl-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-(4-6 membered heterocycloalkyl)-C 1 -C 6 alkyl-, or optionally substituted -C 1 -C 6 alkyl-(5-6 membered heteroaryl)-C 1 -C 6 alkyl- is optionally substituted by 1-4 substituents each independently selected from halogen, hydroxy, -O-P(O)(OH) 2 , -O-P(O)(R I< R II< )2, amino, (C 1 -C 4 alkyl)amino-, (C 1 -C 4 alkyl)(C 1 -C 4 alkyl)amino-, C 1 -C 4 alkyl, halo(C 1 -C 4 alkyl), halo(C 1 -C 4 alkoxy)-, C 1 -C 4 alkoxy-, hydroxy-(C 2 -C 4 alkoxy)-, -(C 2 -C 4 alkoxy)-O-P(O)(OH) 2 , -(C 2 -C 4 alkoxy)-O-P(O)(RIR II< ) 2 ,and C 1 -C 4 alkoxy-(C 1 -C 4 alkoxy)-; R 3< and R 5< are each independently -CON(R d< )(R f< ), or one of R 3< and R 5< is -CON(R d< )(R f< ), and the other of R 3< and R 5< is H, COOH or -CO 2 (R c< ); R 4< and R 6< are each independently selected from H, halogen, halo(C 1 -C 6 alkyl), halo(C 1 -C 6 alkoxy)-, hydroxy, -O-P(O)(OH) 2 , -O-P(O)(R I< R II< )2, -NH 2 , -NR c< R c< , -NR c< R d< , -COR c< , -CO 2 R c< , -N(R d< )COR c< , -N(R d< )SO 2 R c< , -N(R g< )SO 2 (C 1 -C 2 alkyl)-N(R h< )(R f< ), -N(R g< )CO(C 1 -C 2 alkyl)-N(R h< )(R f< ), optionally substituted (C 1 -C 6 alkyl), optionally substituted (C 1 -C 6 alkyl)oxy-, optionally substituted (C 1 -C 6 alkyl)amino-, and optionally substituted (C 1 -C 6 alkyl)(C 1 -C 4 alkyl)amino-, wherein the (C 1 -C 6 alkyl) of said optionally substituted (C 1 -C 6 alkyl), optionally substituted (C 1 -C 6 alkyl)oxy-, optionally substituted (C 1 -C 6 alkyl)amino- and optionally substituted (C 1 -C 6 alkyl)(C 1 -C 4 alkyl)amino- is optionally substituted by 1-4 substituents each independently selected from -OH, -O-P(O)(OH) 2 , -O-P(O)(R I< R II< ) 2 ,-OR c< , -NH 2 , -NR c< R c< , -NR c< R d< , -CO 2 H, -CO 2 R c< , -OCOR c< , -CO 2 H, -CO 2 R c< , -SOR c< , -SO 2 R c< , -CONH 2 , -CONR c< R d< , -SO 2 NH 2 , -SO 2 NR c< R d< , -OCONH 2 , -OCONR c< R d< , -NR d< COR c< , -NR d< SOR c< , -NR d< CO 2 R c< , -NR d< SO 2 R c< , optionally substituted phenyl, optionally substituted 5-6 membered heterocycloalkyl and optionally substituted 5-6 membered heteroaryl group, wherein said optionally substituted phenyl, 5-6 membered heterocycloalkyl or 5-6 membered heteroaryl is optionally substituted by 1-4 substituents each independently selected from halogen, hydroxy, -O-P(O)(OH) 2 , -O-P(O)(R I< R II< )2, amino, (C 1 -C 4 alkyl)amino-, (C 1 -C 4 alkyl)(C 1 -C 4 alkyl)amino-, C 1 -C 4 alkyl, halo(C 1 -C 4 alkyl), hydroxy-(C 1 -C 4 alkyl)-, -(C 1 -C 4 alkyl)-O-P(O)(OH) 2 , -(C 1 -C 4 alkyl)-O-P(O)(R I< R II< ) 2 , halo(C 1 -C 4 alkoxy)-, C 1 -C 4 alkoxy-, hydroxy-(C 2 -C 4 alkoxy)-, -(C 2 -C 4 alkoxy)-O-P(O)(OH) 2 , -(C 2 -C 4 alkoxy)-O-P(O)(R I< R II< ) 2 , C 1 -C 4 alkoxy-(C 1 -C 4 alkoxy)-, -COR d< , -CON(R d< )(R f< ), and -CO 2 R d< ; R 14< is optionally substituted C 1 -C 4 alkyl, wherein said optionally substituted C 1 -C 4 alkyl is optionally substituted by a substituent selected from -OR c< , -NR c< R d< , -CO 2 R c< , -CONR c< R d< , -SO 2 NR c< R d< , and -OCONR c< R d< ; R 16< is H, halogen, or C 1 -C 4 alkyl; R 15< and R 17< are each independently H, cyclopropyl, or C 1 -C 4 alkyl; R a< is H, -R c< , -COR c< , -CO 2 H, -CO 2 R c< , -SOR c< , -SO 2 R c< , -CONH 2 , -CONR c< R d< , -SO 2 NH 2 , or -SO 2 NR c< R d< ; each R b< is independently C 1 -C 4 alkyl, halo(C 1 -C 4 alkyl), -(C 1 -C 4 alkyl)-OH, -(C 1 -C 4 alkyl)-O-P(O)(OH) 2 , -(C 1 -C 4 alkyl)-O-P(O)(R I< R II< ) 2 , -(C 1 -C 4 alkyl)-O-(C 1 -C 4 alkyl), -(C 1 -C 4 alkyl)-N(R e< )(R f< ), -(C 1 -C 4 alkyl)-O-CO(C 1 -C 4 alkyl), or -(C 1 -C 4 alkyl)-CO-O-(C 1 -C 4 alkyl); each R c< is independently C 1 -C 4 alkyl, halo(C 1 -C 4 alkyl), -(C 1 -C 4 alkyl)-OH, -(C 1 -C 4 alkyl)-O-P(O)(OH) 2 , -(C 1 -C 4 alkyl)-O-P(O)(R I< R II< ) 2 ,-(C 1 -C 4 alkyl)-O-(C 1 -C 4 alkyl), -(C 1 -C 4 alkyl)-N(R e< )(R f< ), -(C 1 -C 4 alkyl)-O-CO(C 1 -C 4 alkyl), -(C 1 -C 4 alkyl)-CO-O-(C 1 -C 4 alkyl), optionally substituted C 3 -C 6 cycloalkyl, optionally substituted phenyl, optionally substituted 4-6 membered heterocycloalkyl, optionally substituted 5-6 membered heteroaryl, optionally substituted 9-10 membered heteroaryl, optionally substituted -C 1 -C 4 alkyl-C 3 -C 6 cycloalkyl, optionally substituted -C 1 -C 4 alkyl-phenyl, optionally substituted -C 1 -C 4 alkyl-4-6 membered heterocycloalkyl, optionally substituted -C 1 -C 4 alkyl-5-6 membered heteroaryl, or optionally substituted -C 1 -C 4 alkyl-9-10 membered heteroaryl, wherein the C 3 -C 6 cycloalkyl, phenyl, 4-6 membered heterocycloalkyl, 5-6 membered heteroaryl or 9-10 membered heteroaryl moiety of said optionally substituted C 3 -C 6 cycloalkyl, optionally substituted phenyl, optionally substituted 4-6 membered heterocycloalkyl, optionally substituted 5-6 membered heteroaryl, optionally substituted 9-10 membered heteroaryl optionally substituted -C 1 -C 4 alkyl-C 3 -C 6 cycloalkyl, optionally substituted -C 1 -C 4 alkyl-phenyl, optionally substituted -C 1 -C 4 alkyl-4-6 membered heterocycloalkyl, optionally substituted -C 1 -C 4 alkyl-5-6 membered heteroaryl, or optionally substituted -C 1 -C 4 alkyl-9-10 membered heteroaryl is optionally substituted by 1-4 substituents each independently selected from halogen, hydroxy, -O-P(O)(OH) 2 , -O-P(O)(R I< R II< ) 2 , amino, -(C 1 -C 4 alkyl)NH 2 , (C 1 -C 4 alkyl)amino-, (C 1 -C 4 alkyl)(C 1 -C 4 alkyl)amino-, C 1 -C 4 alkyl, halo(C 1 -C 4 alkyl), halo(C 1 -C 4 alkoxy)-, C 1 -C 4 alkoxy-, hydroxy-(C 2 -C 4 alkoxy)-, -(C 2 -C 4 alkoxy)-O-P(O)(OH) 2 , -(C 2 -C 4 alkoxy)-O-P(O)(R I< R II< ) 2 , C 1 -C 4 alkoxy-(C 1 -C 4 alkoxy)-, -COR d< , -CON(R d< )(R f< ), and -CO 2 R d< ; each R d< is independently H or C 1 -C 4 alkyl; each R e< is independently H, (C 1 -C 4 alkyl), -CO(C 1 -C 4 alkyl), -OCO(C 1 -C 4 alkyl), -CO 2 (C 1 -C 4 alkyl), -(C 1 -C 4 alkyl)NH 2 , -(C 1 -C 4 alkyl) C 1 -C 4 alkoxy, -CO-(optionally substituted 5-6 membered heterocycloalkyl), -CO(C 1 -C 4 alkyl)-(optionally substituted 5-6 membered heterocycloalkyl), -CO(optionally substituted 5-6 membered heteroaryl), -CO(C 1 -C 4 alkyl)-(optionally substituted 5-6 membered heteroaryl), wherein the optionally substituted 5-6 membered heterocycloalkyl or optionally substituted 5-6 membered heteroaryl is optionally substituted 1-4 substituents each independently selected from halogen, hydroxy, -O-P(O)(OH) 2 , -O-P(O)(R I< R II< ) 2 , amino, (C 1 -C 4 alkyl)amino-,(C 1 -C 4 alkyl)(C 1 -C 4 alkyl)amino-, C 1 -C 4 alkyl, halo(C 1 -C 4 alkyl), halo(C 1 -C 4 alkoxy)-, C 1 -C 4 alkoxy-, hydroxy-(C 2 -C 4 alkoxy)-, -(C 2 -C 4 alkoxy) O-P(O)(OH) 2 , -(C 2 -C 4 alkoxy)-O-P(O)(R I< R II< ) 2 , C 1 -C 4 alkoxy-(C 1 -C 4 alkoxy)-, -COR d< , -CON(R d< )(R f< ), and -CO 2 R d< ; each R f< is independently H or (C 1 -C 4 alkyl); R g< and R h< are each independently H or (C 1 -C 4 alkyl) or R g< and R h< , taken together with the atom or atoms through which they are connected, form a 5-6 membered ring; and each occurrence of R I< and R II< are independently (C 1 -C 6 alkyl)oxy-; or a tautomer thereof; or a salt thereof, particularly a pharmaceutically acceptable salt, thereof.

[0027] Further disclosed are compounds of Formula (I) wherein: q is 0 or 1; r is 0 or 1; s is 0 or 1; wherein q + r + s = 1 or 2; when q is 0, R A1< and R A2< are each independently H, halogen, hydroxy, -N(R e< )(R f< ), -CO 2 R f< , -N(R f< )COR b< , -N(R g< )SO 2 (C 1 -C 4 alkyl)-N(R e< )(R f< ), -N(R g< )CO(C 1 -C 4 alkyl)-N(R h< )(R f< ), optionally substituted (C 1 -C 6 alkyl), optionally substituted (C 1 -C 6 alkyl)oxy-, optionally substituted (C 1 -C 6 alkyl)amino-, and optionally substituted (C 1 -C 6 alkyl)(C 1 -C 4 alkyl)amino-, wherein the (C 1 -C 6 alkyl) of said optionally substituted (C 1 -C 6 alkyl), optionally substituted (C 1 -C 6 alkyl)oxy-, optionally substituted (C 1 -C 6 alkyl)amino- and optionally substituted (C 1 -C 6 alkyl)(C 1 -C 4 alkyl)amino- is optionally substituted by 1-4 substituents each independently selected from hydroxy, C 1 -C 4 alkoxy-, -N(R e< )(R f< ), -CO 2 (R f< ), -CON(R e< )(R f< ), optionally substituted phenyl, optionally substituted 5-6 membered heterocycloalkyl and optionally substituted 5-6 membered heteroaryl group, wherein said optionally substituted phenyl, 5-6 membered heterocycloalkyl or 5-6 membered heteroaryl is optionally substituted by 1-4 substituents each independently selected from halogen, hydroxy, amino, (C 1 -C 6 alkyl)amino-, (C 1 -C 6 alkyl)(C 1 -C 6 alkyl)amino-, halo(C 1 -C 6 alkyl), hydroxy-(C 1 -C 4 alkyl)-, halo(C 1 -C 4 alkoxy)-, C 1 -C 4 alkoxy-, hydroxy-(C 2 -C 4 alkoxy)-, and C 1 -C 4 alkoxy-(C 1 -C 4 alkoxy)-; when r is 0, R B1< and R B2< are each independently H, optionally substituted C 1 -C 6 alkyl, halo(C 1 -C 6 alkyl), optionally substituted C 2 -C 6 alkenyl, optionally substituted C 2 -C 6 alkynyl, optionally substituted C 3 -C 6 cycloalkyl, optionally substituted 4-6 membered heterocycloalkyl, optionally substituted phenyl, optionally substituted 5-6 membered heteroaryl, or optionally substituted 9-10 membered heteroaryl, wherein said optionally substituted C 1 -C 6 alkyl, optionally substituted C 2 -C 6 alkenyl, optionally substituted C 2 -C 6 alkynyl, optionally substituted C 3 -C 6 cycloalkyl, optionally substituted 4-6 membered heterocycloalkyl, optionally substituted phenyl, optionally substituted 5-6 membered heteroaryl, or optionally substituted 9-10 membered heteroaryl is optionally substituted by 1-4 substituents each independently selected from halogen, nitro, -R c< , -OH, -OR c< , -NH 2 , -NR c< R c< , -NR c< R d< , -OCOR c< , -CO 2 H, -CO 2 R c< , -SOR c< , -SO 2 R c< , -CONH 2 , -CONR c< R d< , -SO 2 NH 2 , -SO 2 NR c< R d< , -OCONH 2 , -OCONR c< R d< , -NR d< COR c< , -NR d< SOR c< , -NR d< CO 2 R c< , and -NR d< SO 2 R c< ; when s is 0, R C1< is H, halogen, or C 1 -C 4 alkyl and R C2< is optionally substituted C 1 -C 4 alkyl, wherein said optionally substituted C 1 -C 4 alkyl group is optionally substituted by a substituent selected from -OR c< , -NR c< R d< , -CO 2 R c< , -CONR c< R d< , -SO 2 NR c< R d< , and -OCONR c< R d< ; when q is 1, R A1< and R A2< are each independently -CH 2 -, -NR e-< , or -O-, and A, taken together with R A1< and R A2< , forms a linking group, wherein A is -halo(C 1 -C 12 alkyl)-, optionally substituted -C 1 -C 12 alkyl-, optionally substituted -C 2 -C 12 alkenyl-, optionally substituted -C 2 -C 12 alkynyl-, optionally substituted -C 1 -C 6 alkyl-O-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-NR a-< C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-(C 3 -C 6 cycloalkyl)-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-phenyl-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-(4-6 membered heterocycloalkyl)-C 1 -C 6 alkyl-, or optionally substituted -C 1 -C 6 alkyl-(5-6 membered heteroaryl)-C 1 -C 6 alkyl-, wherein the alkyl moiety of said optionally substituted -C 1 -C 12 alkyl-, optionally substituted -C 2 -C 12 alkenyl-, optionally substituted -C 2 -C 12 alkynyl-, optionally substituted -C 1 -C 6 alkyl-O-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-NR a-< C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-(C 3 -C 6 cycloalkyl)-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-phenyl-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-(4-6 membered heterocycloalkyl)-C 1 -C 6 alkyl-, or optionally substituted -C 1 -C 6 alkyl-(5-6 membered heteroaryl)-C 1 -C 6 alkyl- is optionally substituted by 1-4 substituents each independently selected from halogen, halo(C 1 -C 4 alkyl), -OH, -OR c< , -NH 2 , -NR c< R d< , -OCOR c< , -COzH, -CO 2 R c< , -SOR c< , -SO 2 R c< , -CONHz, -CONR c< R d< , -SO 2 NH 2 , -SO 2 NR c< R d< , -O CONH 2 , -OCONR c< R d< , -NR d< COR c< , -NR d< SOR c< , -NR d< CO 2 R c< , and -NR d< SO 2 R c< , and the C 3 -C 6 cycloalkyl, phenyl, 4-6 membered heterocycloalkyl, or 5-6 membered heteroaryl moiety of said optionally substituted -C 1 -C 6 alkyl-(C 3 -C 6 cycloalkyl)-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-phenyl-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-(4-6 membered heterocycloalkyl)-C 1 -C 6 alkyl-, or optionally substituted -C 1 -C 6 alkyl-(5-6 membered heteroaryl)-C 1 -C 6 alkyl- is optionally substituted by 1-4 substituents each independently selected from halogen, hydroxy, amino, (C 1 -C 4 alkyl)amino-, (C 1 -C 4 alkyl)(C 1 -C 4 alkyl)amino-, C 1 -C 4 alkyl, halo(C 1 -C 4 alkyl), halo(C 1 -C 4 alkoxy)-, C 1 -C 4 alkoxy-, hydroxy-(C 2 -C 4 alkoxy)-, and C 1 -C 4 alkoxy-(C 1 -C 4 alkoxy)-; when r is 1, R B1< and R B2< are each independently -CH 2 -, and B, taken together with R B1< and R B2< , forms a linking group, wherein B is a bond or B is -halo(C 1 -C 10 alkyl)-, optionally substituted -C 1 -C 10 alkyl-, optionally substituted -C 2 -C 10 alkenyl-, optionally substituted -C 2 -C 10 alkynyl-, optionally substituted -C 1 -C 6 alkyl-O-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-NR a-< C 1 -C 6 alkyl-, optionally substituted C 3 -C 6 cycloalkyl, optionally substituted phenyl, optionally substituted 4-6 membered heterocycloalkyl, optionally substituted 5-6 membered heteroaryl, optionally substituted -C 1 -C 4 alkyl-(C 3 -C 6 cycloalkyl)-C 1 -C 4 alkyl-, optionally substituted -C 1 -C 4 alkyl-phenyl-C 1 -C 4 alkyl-, optionally substituted -C 1 -C 4 alkyl-(4-6 membered heterocycloalkyl)-C 1 -C 4 alkyl-, or optionally substituted -C 1 -C 4 alkyl-(5-6 membered heteroaryl)-C 1 -C 4 alkyl-, wherein the alkyl moiety of said optionally substituted -C 1 -C 10 alkyl-, optionally substituted -C 2 -C 10 alkenyl-, optionally substituted -C 2 -C 10 alkynyl-, optionally substituted -C 1 -C 6 alkyl-O-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-NR a-< C 1 -C 6 alkyl-, optionally substituted -C 1 -C 4 alkyl-(C 3 -C 6 cycloalkyl)-C 1 -C 4 alkyl-,optionally substituted -C 1 -C 4 alkyl-phenyl-C 1 -C 4 alkyl-, optionally substituted -C 1 -C 4 alkyl-(4-6membered heterocycloalkyl)-C 1 -C 4 alkyl-, or optionally substituted -C 1 -C 4 alkyl-(5-6 membered heteroaryl-C 1 -C 4 alkyl)- is optionally substituted by 1 or 2 substituents each independently selected from halogen, halo(C 1 -C 4 alkyl), -OH, -OR c< , -NH 2 , -NR c< R d< , -OCOR c< , -CO 2 H, -CO 2 R c< , -SOR c< , -SO 2 R c< , -CONH 2 , -CONR c< R d< , -SO 2 NH 2 , -SO 2 NR c< R d< , -OCONH 2 , -OCONR c< R d< , -NR d< COR c< , -NR d< SOR c< , -NR d< CO 2 R c< , and -NR d< SO 2 R c< , and the C 3 -C 6 cycloalkyl, phenyl, 4-6 membered heterocycloalkyl, or 5-6 membered heteroaryl moiety of said optionally substituted C 3 -C 6 cycloalkyl, optionally substituted phenyl, optionally substituted 4-6 membered heterocycloalkyl, optionally substituted 5-6 membered heteroaryl, optionally substituted -C 1 -C 4 alkyl-(C 3 -C 6 cycloalkyl)-C 1 -C 4 alkyl-,optionally substituted -C 1 -C 4 alkyl-phenyl-C 1 -C 4 alkyl-, optionally substituted -C 1 -C 4 alkyl-(4-6membered heterocycloalkyl)-C 1 -C 4 alkyl-, or optionally substituted -C 1 -C 4 alkyl-(5-6 membered heteroaryl)-C 1 -C 4 alkyl- is optionally substituted by 1-4 substituents each independently selected from halogen, hydroxy, amino, (C 1 -C 4 alkyl)amino-, (C 1 -C 4 alkyl)(C 1 -C 4 alkyl)amino-, C 1 -C 4 alkyl, halo(C 1 -C 4 alkyl), halo(C 1 -C 4 alkoxy)-, C 1 -C 4 alkoxy-, hydroxy-(C 2 -C 4 alkoxy)-, and C 1 -C 4 alkoxy-(C 1 -C 4 alkoxy)-; when s is 1, R C1< and R C2< are each independently -CH 2 -, and C, taken together with R C1< and R C2< , forms a linking group, wherein C is -halo(C 1 -C 12 alkyl)-, optionally substituted -C 1 -C 12 alkyl-, optionally substituted -C 2 -C 12 alkenyl-, optionally substituted -C 2 -C 12 alkynyl-, optionally substituted -C 1 -C 6 alkyl-O-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-NR a-< C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-(C 3 -C 6 cycloalkyl)-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-phenyl-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-(4-6 membered heterocycloalkyl)-C 1 -C 6 alkyl-, or optionally substituted -C 1 -C 6 alkyl-(5-6 membered heteroaryl)-C 1 -C 6 alkyl-, wherein the alkyl moiety of said optionally substituted -C 1 -C 12 alkyl-, optionally substituted -C 2 -C 12 alkenyl-, optionally substituted -C 2 -C 12 alkynyl-, optionally substituted -C 1 -C 6 alkyl-O-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-NR a-< C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-(C 3 -C 6 cycloalkyl)-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-phenyl-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-(4-6 membered heterocycloalkyl)-C 1 -C 6 alkyl-, or optionally substituted -C 1 -C 6 alkyl-(5-6 membered heteroaryl)-C 1 -C 6 alkyl- is optionally substituted by 1 or 2 substituents each independently selected from halogen, halo(C 1 -C 4 alkyl), -OH, -OR c< , -NH 2 , -NR c< R d< , -OCOR c< , -CO 2 H, -CO 2 R c< , -SOR c< , -SO 2 R c< , -CONH 2 , -CONR c< R d< , -SO 2 NH 2 , -SO 2 NR c< R d< , -OCONH 2 , -OCONR c< R d< , -NR d< COR c< , -NR d< SOR c< , -NR d< CO 2 R c< , and -NR d< SO 2 R c< , and the C 3 -C 6 cycloalkyl, phenyl, 4-6 membered heterocycloalkyl, or 5-6 membered heteroaryl moiety of said optionally substituted -C 1 -C 6 alkyl-(C 3 -C 6 cycloalkyl)-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-phenyl-C 1 -C 6 alkyl-, optionally substituted -C 1 -C 6 alkyl-(4-6 membered heterocycloalkyl)-C 1 -C 6 alkyl-, or optionally substituted -C 1 -C 6 alkyl-(5-6 membered heteroaryl)-C 1 -C 6 alkyl- is optionally substituted by 1-4 substituents each independently selected from halogen, hydroxy, amino, (C 1 -C 4 alkyl)amino-, (C 1 -C 4 alkyl)(C 1 -C 4 alkyl)amino-, C 1 -C 4 alkyl, halo(C 1 -C 4 alkyl), halo(C 1 -C 4 alkoxy)-, C 1 -C 4 alkoxy-, hydroxy-(C 2 -C 4 alkoxy)-, and C 1 -C 4 alkoxy-(C 1 -C 4 alkoxy)-; R 3< and R 5< are each independently -CON(R d< )(R f< ), or one of R 3< and R 5< is -CON(R d< )(R f< ), and the other of R 3< and R 5< is H or -CO 2 (R c< ); R 4< and R 6< are each independently selected from H, halogen, halo(C 1 -C 6 alkyl), halo(C 1 -C 6 alkoxy)-, hydroxy, -NH 2 , -NR c< R c< , -NR c< R d< , -COR c< , -CO 2 R c< , -N(R d< )COR c< , -N(R d< )SO 2 R c< , -N(R g< )SO 2 (C 1 -C 2 alkyl)-N(R h< )(R f< ), -N(R 9< )CO(C 1 -C 2 alkyl)-N(R h< )(R f< ), optionally substituted (C 1 -C 6 alkyl), optionally substituted (C 1 -C 6 alkyl)oxy-, optionally substituted (C 1 -C 6 alkyl)amino-, and optionally substituted (C 1 -C 6 alkyl)(C 1 -C 4 alkyl)amino-, wherein the (C 1 -C 6 alkyl) of said optionally substituted (C 1 -C 6 alkyl), optionally substituted (C 1 -C 6 alkyl)oxy-, optionally substituted (C 1 -C 6 alkyl)amino- and optionally substituted (C 1 -C 6 alkyl)(C 1 -C 4 alkyl)amino- is optionally substituted by 1-4 substituents each independently selected from -OH, -OR c< , -NH 2 , -NR c< R c< , -NR c< R d< , -CO 2 H, -CO 2 R c< , -OCOR c< , -CO 2 H, -CO 2 R c< , -SOR c< , -SO 2 R c< , -CONH 2 , -CONR c< R d< , -SO 2 NH 2 , -SO 2 NR c< R d< , -OCONH 2 , -OCONR c< R d< , -NR d< COR c< , -NR d< SOR c< , -NR d< CO 2 R c< , -NR d< SO 2 R c< , optionally substituted phenyl, optionally substituted 5-6 membered heterocycloalkyl and optionally substituted 5-6 membered heteroaryl group, wherein said optionally substituted phenyl, 5-6 membered heterocycloalkyl or 5-6 membered heteroaryl is optionally substituted by 1-4 substituents each independently selected from halogen, hydroxy, amino, (C 1 -C 4 alkyl)amino-,(C 1 -C 4 alkyl)(C 1 -C 4 alkyl)amino-, C 1 -C 4 alkyl, halo(C 1 -C 4 alkyl), hydroxy-(C 1 -C 4 alkyl)-, halo(C 1 -C 4 alkoxy)-, C 1 -C 4 alkoxy-, hydroxy-(C 2 -C 4 alkoxy)-, C 1 -C 4 alkoxy-(C 1 -C 4 alkoxy)-, -COR d< , -CON(R d< )(R f< ), and -CO 2 R d< ; R 14< is optionally substituted C 1 -C 4 alkyl, wherein said optionally substituted C 1 -C 4 alkyl is optionally substituted by a substituent selected from -OR c< , -NR c< R d< , -CO 2 R c< , -CONR c< R d< , -SO 2 NR c< R d< , and -OCONR c< R d< ; R 16< is H, halogen, or C 1 -C 4 alkyl; R 15< and R 17< are each independently H, cyclopropyl, or C 1 -C 4 alkyl; R a< is H, -R c< , -COR c< , -CO 2 H, -CO 2 R c< , -SOR c< , -SO 2 R c< , -CONH 2 , -CONR c< R d< , -SO 2 NH 2 , or -SO 2 NR c< R d< ; each R b< is independently C 1 -C 4 alkyl, halo(C 1 -C 4 alkyl), -(C1-C4alkyl)-OH, -(C 1 -C 4 alkyl)-O-(C 1 -C 4 alkyl), -(C 1 -C 4 alkyl)-N(R e< )(R f< ), -(C 1 -C 4 alkyl)-O-CO(C 1 -C 4 alkyl), or -(C 1 -C 4 alkyl)-CO-O-(C 1 -C 4 alkyl); each R c< is independently C 1 -C 4 alkyl, halo(C 1 -C 4 alkyl), -(C 1 -C 4 alkyl)-OH, -(C 1 -C 4 alkyl)-O-(C 1 -C 4 alkyl), -(C 1 -C 4 alkyl)-N(R e< )(R f< ), -(C 1 -C 4 alkyl)-O-CO(C 1 -C 4 alkyl), -(C 1 -C 4 alkyl)-CO-O-(C 1 -C 4 alkyl), optionally substituted C 3 -C 6 cycloalkyl, optionally substituted phenyl, optionally substituted 4-6 membered heterocycloalkyl, optionally substituted 5-6 membered heteroaryl, optionally substituted 9-10 membered heteroaryl, optionally substituted -C 1 -C 4 alkyl-C 3 -C 6 cycloalkyl, optionally substituted -C 1 -C 4 alkyl-phenyl, optionally substituted -C 1 -C 4 alkyl-4-6 membered heterocycloalkyl, optionally substituted -C 1 -C 4 alkyl-5-6 membered heteroaryl, or optionally substituted -C 1 -C 4 alkyl-9-10 membered heteroaryl, wherein the C 3 -C 6 cycloalkyl, phenyl, 4-6 membered heterocycloalkyl, 5-6 membered heteroaryl or 9-10 membered heteroaryl moiety of said substituted C 3 -C 6 cycloalkyl, optionally substituted phenyl, optionally substituted 4-6 membered heterocycloalkyl, optionally substituted 5-6 membered heteroaryl, optionally substituted 9-10 membered heteroaryl optionally substituted -C 1 -C 4 alkyl-C 3 -C 6 cycloalkyl, optionally substituted -C 1 -C 4 alkyl-phenyl, optionally substituted -C 1 -C 4 alkyl-4-6 membered heterocycloalkyl, optionally substituted -C 1 -C 4 alkyl-5-6 membered heteroaryl, or optionally substituted -C 1 -C 4 alkyl-9-10 membered heteroaryl is optionally substituted by 1-4 substituents each independently selected from halogen, hydroxy, amino, (C 1 -C 4 alkyl)amino-, (C 1 -C 4 alkyl)(C 1 -C 4 alkyl)amino-, C 1 -C 4 alkyl, halo(C 1 -C 4 alkyl), halo(C 1 -C 4 alkoxy)-, C 1 -C 4 alkoxy-, hydroxy-(C 2 -C 4 alkoxy)-, C 1 -C 4 alkoxy-(C 1 -C 4 alkoxy)-, -COR d< , -CON(R d< )(R f< ), and -CO 2 R d< ; each R d< is independently H or C 1 -C 4 alkyl; each R e< is independently H, (C 1 -C 4 alkyl), -CO(C 1 -C 4 alkyl), -OCO(C 1 -C 4 alkyl), -CO 2 (C 1 -C 4 alkyl), -CO-(optionally substituted 5-6 membered heterocycloalkyl), -CO(C 1 -C 4 alkyl)-(optionally substituted 5-6 membered heterocycloalkyl), -CO(optionally substituted 5-6 membered heteroaryl), -CO(C 1 -C 4 alkyl)-(optionally substituted 5-6 membered heteroaryl), wherein the optionally substituted 5-6 membered heterocycloalkyl or optionally substituted 5-6 membered heteroaryl is optionally substituted 1-4 substituents each independently selected from halogen, hydroxy, amino, (C 1 -C 4 alkyl)amino-, (C 1 -C 4 alkyl)(C 1 -C 4 alkyl)amino-, C 1 -C 4 alkyl, halo(C 1 -C 4 alkyl), halo(C 1 -C 4 alkoxy)-, C 1 -C 4 alkoxy-, hydroxy-(C 2 -C 4 alkoxy)-, C 1 -C 4 alkoxy-(C 1 -C 4 alkoxy)-, -COR d< , -CON(R d< )(R f< ), and -CO 2 R d< ; each R f< is independently H or (C 1 -C 4 alkyl); R g< and R h< are each independently H or (C 1 -C 4 alkyl) or R g< and R h< , taken together with the atom or atoms through which they are connected, form a 5-6 membered ring; or a tautomer thereof; or a salt, particularly a pharmaceutically acceptable salt, thereof.

[0028] The alternative definitions for the various groups and substituent groups of Formula (I-N), (I) or Formula (I-P) provided throughout the specification are intended to particularly describe each compound species disclosed herein, individually, as well as groups of one or more compound species.

[0029] It will be appreciated by those skilled in the art that the compounds of this invention may exist in other tautomeric forms including zwitterionic forms, or isomeric forms. All tautomeric (including zwitterionic forms) and isomeric forms of the compounds of the invention described herein are intended to be encompassed within the scope of the present invention.

[0030] It will also be appreciated by those skilled in the art that the compounds of this invention may exist in tautomeric forms including, but not limited to, Formula (A), Formula (B) and / or Formula (C) or zwitterionic forms including, but not limited to, Formula (D) or Formula (E).

[0031] The chemical names provided for the intermediate compounds and / or the compounds of this invention described herein may refer to any one of the tautomeric representations of such compounds (in some instances, such alternate names are provided with the experimental). It is to be understood that any reference to a named compound (an intermediate compound or a compound of the invention) or a structurally depicted compound (an intermediate compound or a compound of the invention) is intended to encompass all tautomeric forms including zwitterionic forms of such compounds and any mixture thereof.

[0032] As used herein, the term "alkyl" represents a saturated, straight or branched hydrocarbon group having the specified number of carbon atoms. The term "C 1 -C 4 alkyl" refers to a straight or branched alkyl moiety containing from 1 to 4 carbon atoms. Exemplary alkyls include, but are not limited to methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, s-butyl, t-butyl, pentyl and hexyl.

[0033] When a substituent term such as "alkyl" is used in combination with another substituent term, for example as in "hydroxy(C 1 -C 4 alkyl)", the linking substituent term (e.g., alkyl) is intended to encompass a divalent moiety, wherein the point of attachment is through that linking substituent. Examples of "hydroxy(C 1 -C 4 alkyl)" groups include, but are not limited to, hydroxymethyl, hydroxyethyl, and hydroxyisopropyl.

[0034] As used herein, the term "halo(alkyl)" represents a saturated, straight or branched hydrocarbon group having the specified number (n) of carbon atoms and one or more (up to 2n+1) halogen atoms. For example, the term "halo(C 1 -C 4 alkyl)" represents a group having one or more halogen atoms, which may be the same or different, at one or more carbon atoms of an alkyl moiety containing from 1 to 4 carbon atoms. Examples of "halo(C 1 -C 4 alkyl)" groups include, but are not limited to, -CF 3 (trifluoromethyl), -CCl 3 (trichloromethyl), 1,1-difluoroethyl, 2,2,2-trifluoroethyl, and hexafluoroisopropyl.

[0035] "Alkenyl" refers to straight or branched hydrocarbon group having the specified number of carbon atoms and at least 1 and up to 3 carbon-carbon double bonds. Examples include ethenyl and propenyl.

[0036] "Alkynyl" refers to straight or branched hydrocarbon group having the specified number of carbon atoms and at least 1 and up to 3 carbon-carbon triple bonds. Examples include ethynyl and propynyl.

[0037] "Alkoxy-" or "(alkyl)oxy-" refers to an "alkyl-oxy-" group, containing an alkyl moiety, having the specified number of carbon atoms, attached through an oxygen linking atom. For example, the term "C 1 -C 4 alkoxy-" represents a saturated, straight or branched hydrocarbon moiety having at least 1 and up to 4 carbon atoms attached through an oxygen linking atom. Exemplary "C 1 -C 4 alkoxy-" or "(C 1 -C 4 alkyl)oxy-" groups include, but are not limited to, methoxy, ethoxy, n-propoxy, isopropoxy, n-butoxy, s-butoxy, and t-butoxy.

[0038] As used herein, the term "halo(alkoxy)-" represents a saturated, straight or branched hydrocarbon group having the specified number (n) of carbon atoms and one or more (up to 2n+1) halogen atoms, attached through an oxygen linking atom. For example, the term "halo(C 1 -C 4 alkoxy)-" refers to a "haloalkyl-oxy-" group, containing a "halo(C 1 -C 4 alkyl)" moiety attached through an oxygen linking atom. Exemplary "halo(C 1 -C 4 alkoxy)-" groups include, but are not limited to, -OCHF 2 (difluoromethoxy), -OCF 3 (trifluoromethoxy), -OCH 2 CF 3 (trifluoroethoxy), and -OCH(CF 3 ) 2 (hexafluoroisopropoxy).

[0039] A carbocyclic group or moiety is a cyclic group or moiety in which the ring members are carbon atoms, which may be saturated, partially unsaturated (non-aromatic) or fully unsaturated (aromatic).

[0040] "Cycloalkyl" refers to a non-aromatic, saturated, hydrocarbon ring group containing the specified number of carbon atoms in the ring. For example, the term "C 3 -C 6 cycloalkyl" refers to a cyclic group having from three to six ring carbon atoms. Exemplary "C 3 -C 6 cycloalkyl" groups include cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

[0041] A heterocyclic group or moiety is a cyclic group or moiety having, as ring members, atoms of at least two different elements, which cyclic group or moiety may be saturated, partially unsaturated (non-aromatic) or fully unsaturated (aromatic).

[0042] "Heteroatom" refers to a nitrogen, sulfur, or oxygen atom, for example a nitrogen atom or an oxygen atom.

[0043] "Heterocycloalkyl" refers to a non-aromatic, monocyclic or bicyclic group containing 3-10 ring atoms and containing one or more (generally one or two) heteroatom ring members independently selected from oxygen, sulfur, and nitrogen. The point of attachment of a heterocycloalkyl group may be by any suitable carbon or nitrogen atom.

[0044] Examples of "heterocycloalkyl" groups include, but are not limited to, aziridinyl, thiiranyl, oxiranyl, azetidinyl, oxetanyl, thietanyl, pyrrolidinyl, tetrahydrofuranyl, tetrahydrothienyl, 1,3-dioxolanyl, piperidinyl, piperazinyl, tetrahydropyranyl, dihydropyranyl, tetrahydrothiopyranyl, 1,3-dioxanyl, 1,4-dioxanyl, 1,3-oxathiolanyl, 1,3-oxathianyl, 1,3-dithianyl, 1,4-oxathiolanyl, 1,4-oxathianyl, 1,4-dithianyl, morpholinyl, thiomorpholinyl, and hexahydro-1H-1,4-diazepinyl. Examples of "4-membered heterocycloalkyl" groups include oxetanyl, thietanyl and azetidinyl.

[0045] The term "5-6 membered heterocycloalkyl" represents a saturated, monocyclic group, containing 5 or 6 ring atoms, which includes one or two heteroatoms selected independently from oxygen, sulfur, and nitrogen. Illustrative examples of 5-6 membered heterocycloalkyl groups include, but are not limited to pyrrolidinyl, tetrahydrofuranyl, tetrahydrothienyl, tetrahydropyranyl, tetrahydrothiopyranyl, piperidinyl, piperazinyl, morpholinyl, and thiomorpholinyl.

[0046] "Heteroaryl" refers to an aromatic monocyclic or bicyclic group containing 5 to 10 ring atoms, including 1 to 4 heteroatoms independently selected from nitrogen, oxygen and sulfur, wherein at least a portion of the group is aromatic. For example, this term encompasses bicyclic heterocyclic-aryl groups containing either a phenyl ring fused to a heterocyclic moiety or a heteroaryl ring moiety fused to a carbocyclic moiety. The point of attachment of a heteroaryl group may be by any suitable carbon or nitrogen atom.

[0047] The term "5-6 membered heteroaryl" represents an aromatic monocyclic group containing 5 or 6 ring atoms, including at least one carbon atom and 1 to 4 heteroatoms independently selected from nitrogen, oxygen and sulfur. Selected 5-membered heteroaryl groups contain one nitrogen, oxygen, or sulfur ring heteroatom, and optionally contain 1, 2, or 3 additional nitrogen ring atoms. Selected 6-membered heteroaryl groups contain 1, 2, or 3 nitrogen ring heteroatoms. Examples of 5-membered heteroaryl groups include furyl (furanyl), thienyl, pyrrolyl, imidazolyl, pyrazolyl, triazolyl, tetrazolyl, thiazolyl, isothiazolyl, thiadiazolyl, oxazolyl, isoxazolyl, and oxadiazolyl. Selected 6-membered heteroaryl groups include pyridinyl (pyridyl), pyrazinyl, pyrimidinyl, pyridazinyl and triazinyl.

[0048] The term "9-10 membered heteroaryl" refers to an aromatic bicyclic group containing 9 or 10 ring atoms, including 1 to 4 heteroatoms independently selected from nitrogen, oxygen and sulfur. Examples of 9-membered heteroaryl (6,5-fused heteroaryl) groups include benzothienyl, benzofuranyl, indolyl, indolinyl (dihydroindolyl), isoindolyl, isoindolinyl, indazolyl, isobenzofuryl, 2,3-dihydrobenzofuryl, benzoxazolyl, benzoisoxazolyl, benzothiazolyl, benzoisothiazolyl, benzimidazolyl, benzoxadiazolyl, benzothiadiazolyl, benzotriazolyl, purinyl, imidazopyridinyl, pyrazolopyridinyl, triazolopyridinyl and 1,3-benzodioxolyl.

[0049] Examples of 10-membered heteroaryl (6,6-fused heteroaryl) groups include quinolinyl (quinolyl), isoquinolyl, phthalazinyl, naphthridinyl (1,5-naphthyridinyl, 1,6-naphthyridinyl, 1,7-naphthyridinyl, 1,8-naphthyridinyl), quinazolinyl, quinoxalinyl, 4H-quinolizinyl, 1,2,3,4-tetrahydroquinolinyl (tetrahydroquinolinyl), 1,2,3,4-tetrahydroisoquinolinyl (tetrahydroisoquinolinyl), cinnolinyl, pteridinyl, and 2,3-dihydrobenzo[b][1,4]dioxinyl.

[0050] The terms "halogen" and "halo" refers to a halogen radical, for example, a fluoro, chloro, bromo, or iodo substituent.

[0051] "Oxo" represents a double-bonded oxygen moiety; for example, if attached directly to a carbon atom forms a carbonyl moiety (C = O).

[0052] "Hydroxy" or "hydroxyl" is intended to mean the radical -OH.

[0053] As used herein, the term "cyano" refers to a nitrile group, -C=N.

[0054] As used herein, the term "optionally substituted" indicates that a group (such as an alkyl, cycloalkyl, alkoxy, heterocycloalkyl, aryl, or heteroaryl group) or ring or moiety may be unsubstituted, or the group, ring or moiety may be substituted with one or more substituent(s) as defined in the substituent definitions (A, R 3< , etc,) provided herein. In the case where groups may be selected from a number of alternative groups, the selected groups may be the same or different.

[0055] The term "independently" means that where more than one substituent is selected from a number of possible substituents, those substituents may be the same or different.

[0056] The term "pharmaceutically acceptable" refers to those compounds, materials, compositions, and dosage forms which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of human beings and animals without excessive toxicity, irritation, or other problem or complication, commensurate with a reasonable benefit / risk ratio.

[0057] As used herein, the terms "compound(s) of the invention" or "compound(s) of this invention" mean (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide, as defined herein, in any form, i.e., any tautomeric form, any isomeric form, any salt or non-salt form (e.g., as a free acid or base form, or as a salt, particularly a pharmaceutically acceptable salt thereof) and any physical form thereof (e.g., including non-solid forms (e.g., liquid or semi-solid forms), and solid forms (e.g., amorphous or crystalline forms, specific polymorphic forms, solvate forms, including hydrate forms (e.g., mono-, di- and hemi- hydrates)), and mixtures of various forms.

[0058] Accordingly, included within the present invention is (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide, in any salt or non-salt form and any physical form thereof, and mixtures of various forms. While such are included within the present invention, it will be understood that (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide as defined herein, in any salt or non-salt form, and in any physical form thereof, may have varying levels of activity, different bioavailabilities and different handling properties for formulation purposes.

[0059] Representative compounds of this invention include the compound of Example 14. Reference compounds of this disclosure include the compounds of Examples 1 to 13 and 15 to 197. It will be appreciated that the present invention encompasses (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide as the free base and as salts thereof, for example as a pharmaceutically acceptable salt thereof. In one embodiment the invention relates to (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide in the form of a free base. In another embodiment the invention relates to (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide in the form of a salt, particularly, a pharmaceutically acceptable salt. It will be further appreciated that, in one embodiment, the invention relates to (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide in the form of a free base. In another embodiment the invention relates to (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide in the form of a salt, particularly, a pharmaceutically acceptable salt.

[0060] Reference embodiments of the compounds of this disclosure include: 1,1'-((2R,3R)-2,3-dihydroxybutane-1,4-diyl)bis(2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-1H-benzo[d]imidazole-5-carboxamide); (E)-1,1'-(but-2-ene-1,4-diyl)bis(2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-1H-benzo[d]imidazole-5-carboxamide); 1,1'-((methylazanediyl)bis(-ethane-2,1-diyl))bis(2-(1-ethyl-3-methyl-1H-pyrazole-5 carboxamido)-1H-benzo[d]imidazole-5-carboxamide); methyl 1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-1H-benzo[d]imidazol-1-yl)butyl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxylate; 1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-1H-benzo[d]imidazol-1-yl)butyl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide; (E)-1,1'-(but-2-ene-1,4-diyl)bis(2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-hydroxypropoxy)-1H-benzo[d]imidazole-5-carboxamide); 8-ethyl-10,18-dimethyl-7,20-dioxo-6,7,8,11,12,13,14,15,20,21,28,29,30,31-tetradecahydrobenzo[4,5]imidazo[1,2-a]benzo[4,5]imidazo[2,1-p]dipyrazolo[5,1-e:4',3'-1][1,3,6,15,17]pentaazacyclohenicosine-3,24-dicarboxamide; 8-ethyl-10,18,30-trimethyl-7,20-dioxo-7,8,11,12,13,14,15,20,21,28,29,30,31,32-tetradecahydro-1H-benzo[4,5]imidazo[2,1-b]benzo[4,5]imidazo[1,2-i]dipyrazolo[5,1-m:4',3'-t][1,3,6,9,11,14]hexaazacyclodocosine-3,24-dicarboxamide; and 1,15-bis(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-8,9,16,17,18,19-hexahydro-7H-6,10-dioxa-2,14,15a,19a-tetraazacyclopentadeca[1,2,3-cd:11,10,9-c'd']diindene-4,12-dicarboxamide; as a free base, or a tautomer thereof; or a salt thereof, particularly a pharmaceutically acceptable salt, thereof.

[0061] Reference embodiments of the compounds of this disclosure include: (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-hydroxypropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide; (E)-1,1'-(but-2-ene-1,4-diyl)bis(2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazole-5-carboxamide); (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazole-5-carboxamide; (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide; (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-((4-methoxybenzyl)oxy)-1H-benzo[d]imidazole-5-carboxamide; (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-hydroxy-1H-benzo[d]imidazole-5-carboxamide; 1,1'-(2,2,3,3-tetrafluorobutane-1,4-diyl)bis(2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-1H-benzo[d]imidazole-5-carboxamide); di-tert-butyl(3-(((Z)-6-carbamoyl-3-((E)-4-((Z)-5-carbamoyl-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-7-methoxy-2,3-dihydro-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-2,3-dihydro-1H-benzo[d]imidazol-4-yl)oxy)propyl) phosphate; 3-(((Z)-6-carbamoyl-3-((E)-4-((Z)-5-carbamoyl-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-7-methoxy-2,3-dihydro-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-2,3-dihydro-1H-benzo[d]imidazol-4-yl)oxy)propyl dihydrogen phosphate; (E)-7-bromo-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-methoxypropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-1H-benzo[d]imidazole-5-carboxamide; ethyl(E)-3-(5-carbamoyl-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-methoxypropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-1H-benzo[d]imidazol-7 -yl)propanoate; ethyl(E)-3-(5-Carbamoyl-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-methoxypropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-1H-benzo[d]imidazol-7-yl)propanoic acid; methyl-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-1H-benzo[d]imidazol-1-yl)butyl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-1H-benzo[d]imidazole-5-carboxylate; methyl 1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-1H-benzo[d]imidazol-1-yl)butyl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-1H-benzo[d]imidazole-5-carboxylate; 1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-1H-benzo[d]imidazol-1-yl)butyl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-1H-benzo[d]imidazole-5-carboxylic acid; (E)-1,1'-(but-2-ene-1,4-diyl)bis(2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide); 1,1-(butane-1,4-diyl)bis(2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-hydroxypropoxy)-1H-benzo[d]imidazole-5-carboxamide); (E)-8-ethyl-4,26-bis(3-hydroxypropoxy)-10,18-dimethyl-7,20-dioxo-6,7,8,11,12,13,14,15,20,21,28,31-dodecahydrobenzo[4,5]imidazo[1,2-a]benzo[4,5]imidazo[2,1-p]dipyrazolo[5,1-e:4',3'-l][1,3,6,15,17]pentaazacyclohenicosine-2,24-dicarboxamide; (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-hydroxypropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-methoxypropoxy)-1H-benzo[d]imidazole-5-carboxamide; (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-1Hbenzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(2-methoxyethoxy)-1H-benzo[d]imidazole-5-carboxamide; (E)-1,1'-(but-2-ene-1,4-diyl)bis(2-(l-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-isopropoxy-1H-benzo[d]imidazole-5-carboxamide); (E)-7-(benzyloxy)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-1H-benzo[d]imidazole-5-carboxamide; (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-1Hbenzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methyl-1Hbenzo[d]imidazole-5-carboxamide; (E)-1,1'-(but-2-ene-1,4-diyl)bis(7-butoxy-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-1H-benzo[d]imidazole-5-carboxamide); (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-1Hbenzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-isopropoxy-1H-benzo[d]imidazole-5-carboxamide; (E)-1,1'-(but-2-ene-1,4-diyl)bis(2-(l-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-isopropoxypropoxy)-1H-benzo[d]imidazole-5-carboxamide); (E)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-1-(4-(2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(morpholinomethyl)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-1H-benzo[d]imidazole-5-carboxamide; as a free base, or a tautomer thereof; or a salt thereof, particularly a pharmaceutically acceptable salt, thereof.

[0062] One embodiment of the compounds of this invention include: (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide; (E)-1-((E)-4-((E)-5-carbamoyl-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-7-(3-morpholinopropoxy)-2,3-dihydro-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-7-methoxy-2,3-dihydro-1H-benzo[d]imidazole-5-carboxamide; or a tautomer thereof; or a salt thereof, particularly a pharmaceutically acceptable salt, thereof.

[0063] Reference compounds of this disclosure include: (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-hydroxypropoxy)-1H-benzo[d]imidazole-5-carboxamide; (E)-1-((E)-4-((E)-5-carbamoyl-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-2,3-dihydro-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-7-(3-hydroxypropoxy)-2,3-dihydro-1H-benzo[d]imidazole-5-carboxamide; (Z)-1-((E)-4-((Z)-5-carbamoyl-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-2,3-dihydro-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-7-(3-hydroxypropoxy)-2,3-dihydro-1H-benzo[d]imidazole-5-carboxamide; (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-hydroxypropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7 -methoxy-1H-benzo[d]imidazole-5-carboxamide; (E)-1-((E)-4-((E)-5-carbamoyl-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-7-(3-hydroxypropoxy)-2,3-dihydro-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-7-methoxy-2,3-dihydro-1H-benzo[d]imidazole-5-carboxamide; (Z)-1-((E)-4-((Z)-5-carbamoyl-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-7-(3-hydroxypropoxy)-2,3-dihydro-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-7-methoxy-2,3-dihydro-1H-benzo[d]imidazole-5-carboxamide; (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazole-5-carboxamide; (E)-1-((E)-4-((E)-5-carbamoyl-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-2,3-dihydro-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-7-(3-morpholinopropoxy)-2,3-dihydro-1H-benzo[d]imidazole-5-carboxamide; (Z)-1-((E)-4-((Z)-5-carbamoyl-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-2,3-dihydro-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-7-(3-morpholinopropoxy)-2,3-dihydro-1H-benzo[d]imidazole-5-carboxamide; (Z)-1-((E)-4-((Z)-5-carbamoyl-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-7-(3-morpholinopropoxy)-2,3-dihydro-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-7-methoxy-2,3-dihydro-1H-benzo[d]imidazole-5-carboxamide; 3-(((Z)-6-carbamoyl-3-((E)-4-((Z)-5-carbamoyl-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-7-methoxy-2,3-dihydro-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-2,3-dihydro-1H-benzo[d]imidazol-4-yl)oxy)propyldihydrogen phosphate; (E)-3-((5-carbamoyl-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-1H-benzo[d]imidazol-7-yl)oxy)propyl dihydrogen phosphate; 3-(((Z)-6-carbamoyl-3-((E)-4-((Z)-5-carbamoyl-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-7-methoxy-2,3-dihydro-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-2,3-dihydro-1H-benzo[d]imidazol-4-yl)oxy)propyl dihydrogen phosphate; or a tautomer thereof; or a salt thereof, particularly a pharmaceutically acceptable salt, thereof.

[0064] Disclosed herein, is reference compound (E)-1-(4-(5-Carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-hydroxypropoxy)-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof; or a salt thereof, particularly a pharmaceutically acceptable salt, thereof.

[0065] Disclosed herein, is reference compound (E)-l-((E)-4-((E)-5-carbamoyl-2-((l-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-2,3-dihydro-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-7-(3-hydroxypropoxy)-2,3-dihydro-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof; or a salt thereof, particularly a pharmaceutically acceptable salt, thereof.

[0066] Disclosed herein, is reference compound (Z)-1-((E)-4-((Z)-5-carbamoyl-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-2,3-dihydro-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-7-(3-hydroxypropoxy)-2,3-dihydro-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof; or a salt thereof, particularly a pharmaceutically acceptable salt, thereof.

[0067] Disclosed herein, is reference compound (E)-1-(4-(5-Carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-hydroxypropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof; or a salt thereof, particularly a pharmaceutically acceptable salt, thereof.

[0068] Disclosed herein, is reference compound (E)-1-((E)-4-((E)-5-carbamoyl-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-7-(3-hydroxypropoxy)-2,3-dihydro-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-7-methoxy-2,3-dihydro-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof; or a salt thereof, particularly a pharmaceutically acceptable salt, thereof.

[0069] Disclosed herein, is reference compound (Z)-1-((E)-4-((Z)-5-carbamoyl-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-7-(3-hydroxypropoxy)-2,3-dihydro-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-7-methoxy-2,3-dihydro-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof; or a salt thereof, particularly a pharmaceutically acceptable salt, thereof.

[0070] Disclosed herein, is reference compound (E)-1-(4-(5-Carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof; or a salt thereof, particularly a pharmaceutically acceptable salt, thereof.

[0071] Disclosed herein, is reference compound (E)-1-((E)-4-((E)-5-carbamoyl-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-2,3-dihydro-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-7-(3-morpholinopropoxy)-2,3-dihydro-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof; or a salt thereof, particularly a pharmaceutically acceptable salt, thereof.

[0072] Disclosed herein, is reference compound (Z)-1-((E)-4-((Z)-5-carbamoyl-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-2,3-dihydro-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-7-(3-morpholinopropoxy)-2,3-dihydro-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof; or a salt thereof, particularly a pharmaceutically acceptable salt, thereof.

[0073] In one embodiment, the compound of the invention is (E)-1-(4-(5-Carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof; or a salt thereof, particularly a pharmaceutically acceptable salt, thereof.

[0074] In one embodiment, the compound of the invention is (E)-1-((E)-4-((E)-5-carbamoyl-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-7-(3-morpholinopropoxy)-2,3-dihydro-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-7-methoxy-2,3-dihydro-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof; or a salt thereof, particularly a pharmaceutically acceptable salt, thereof.

[0075] Disclosed herein, is reference compound (Z)-1-((E)-4-((Z)-5-carbamoyl-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-7-(3-morpholinopropoxy)-2,3-dihydro-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-7-methoxy-2,3-dihydro-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof; or a salt thereof, particularly a pharmaceutically acceptable salt, thereof.

[0076] Disclosed herein, is reference compound 3-(((Z)-6-Carbamoyl-3-((E)-4-((Z)-5-carbamoyl-2-((l-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-7-methoxy-2,3-dihydro-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-2,3-dihydro-1H-benzo[d]imidazol-4-yl)oxy)propyl dihydrogen phosphate or a tautomer thereof; or a salt thereof, particularly a pharmaceutically acceptable salt, thereof.

[0077] Disclosed herein, is reference compound (E)-3-((5-carbamoyl-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-1H-benzo[d]imidazol-7-yl)oxy)propyl dihydrogen phosphate or a tautomer thereof; or a salt thereof, particularly a pharmaceutically acceptable salt, thereof.

[0078] Disclosed herein, is reference compound 3-(((Z)-6-carbamoyl-3-((E)-4-((Z)-5-carbamoyl-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-7-methoxy-2,3-dihydro-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-2,3-dihydro-1H-benzo[d]imidazol-4-yl)oxy)propyl dihydrogen phosphate or a tautomer thereof; or a salt thereof, particularly a pharmaceutically acceptable salt, thereof.

[0079] The compounds of this invention may contain one or more asymmetric centers (also referred to as a chiral center), such as a chiral carbon, or a chiral -SO- moiety. Compounds of this invention containing one or more chiral centers may be present as racemic mixtures, diastereomeric mixtures, enantiomerically enriched mixtures, diastereomerically enriched mixtures, or as enantiomerically or diastereomerically pure individual stereoisomers.

[0080] The stereochemistry of the chiral center present in compounds of this invention is generally represented in the compound names and / or in the chemical structures illustrated herein. Where the stereochemistry of a chiral center present in a compound of this invention, or in any chemical structure illustrated herein, is not specified, the structure is intended to encompass any stereoisomer and all mixtures thereof. An individual isomer isolated such as to be substantially free of the other isomer (i.e. pure) may be isolated such that less than 10%, particularly less than about 1%, for example less than about 0.1% of the other isomer is present.

[0081] Individual stereoisomers of a compound of this invention may be resolved (or mixtures of stereoisomers may be enriched) using methods known to those skilled in the art. For example, such resolution may be carried out (1) by formation of diastereoisomeric salts, complexes or other derivatives; (2) by selective reaction with a stereoisomer-specific reagent, for example by enzymatic oxidation or reduction; or (3) by gas-liquid or liquid chromatography in a chiral environment, for example, on a chiral support such as silica with a bound chiral ligand or in the presence of a chiral solvent. It will be appreciated that where the desired stereoisomer is converted into another chemical entity by one of the separation procedures described above, a further step is required to liberate the desired form. Alternatively, specific stereoisomers may be synthesized by asymmetric synthesis using optically active reagents, substrates, catalysts or solvents, or by converting one enantiomer to the other by asymmetric transformation.

[0082] The invention also includes various deuterated forms of the compounds of this invention. Each available hydrogen atom attached to a carbon atom may be independently replaced with a deuterium atom. A person of ordinary skill in the art will know how to synthesize deuterated forms of the compounds of this invention. For example, a-deuterated a-amino acids are commercially available or may be prepared by conventional techniques (see for example: Elemes, Y. and Ragnarsson, U. J. Chem. Soc., Perkin Trans. 1, 1996, 6, 537-40). Employing such compounds may allow for the preparation of compounds in which the hydrogen atom at a chiral center is replaced with a deuterium atom. Other commercially available deuterated starting materials may be employed in the preparation of deuterated analogs of the compounds of this invention (see for example: methyl-d 3 -amine available from Aldrich Chemical Co., Milwaukee, WI), or they may be synthesized using conventional techniques employing deuterated reagents (e.g. by reduction using lithium aluminum deuteride or sodium borodeuteride or by metal-halogen exchange followed by quenching with D 2 O or methanol-d 3 ).

[0083] Suitable pharmaceutically acceptable salts of (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide can include acid addition salts or base addition salts. For reviews of suitable pharmaceutically acceptable salts see Berge et al., J. Pharm. Sci., 66:1-19, (1977) and P. H. Stahl and C. G. Wermuth, Eds., Handbook of Pharmaceutical Salts: Properties, Selection and Use, Weinheim / Zürich:Wiley-VCH / VHCA (2002).

[0084] Salts of (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide containing a basic amine or other basic functional group may be prepared by any suitable method known in the art, such as treatment of the free base with a suitable inorganic or organic acid. Examples of pharmaceutically acceptable salts so formed include acetate, adipate, ascorbate, aspartate, benzenesulfonate, benzoate, camphorate, camphor-sulfonate (camsylate), caprate (decanoate), caproate (hexanoate), caprylate (octanoate), carbonate, bicarbonate, cinnamate, citrate, cyclamate, dodecylsulfate (estolate), ethane-1,2-disulfonate (edisylate), ethanesulfonate (esylate), formate, fumarate (hemi-fumarate, etc.), galactarate (mucate), gentisate (2,5-dihydroxybenzoate), glucoheptonate (gluceptate), gluconate, glucuronate, glutamate, glutarate, glycerophosphorate, glycolate, hippurate, hydrobromide, hydrochloride (dihydrochloride, etc.), hydroiodide, isobutyrate, lactate, lactobionate, laurate, maleate, malate, malonate, mandelate, methanesulfonate (mesylate), naphthalene-1,5-disulfonate (napadisylate), naphthalene-sulfonate (napsylate), nicotinate, nitrate, oleate, oxalate, palmitate, pamoate, phosphate (diphosphate, etc.), proprionate, pyroglutamate, salicylate, sebacate, stearate, succinate, sulfate, tartrate, thiocyanate, p-toluenesulfonate (tosylate), undecylenate, 1-hydroxy-2-naphthoate, 2,2-dichloroacetate, 2-hydroxyethanesulfonate (isethionate), 2-oxoglutarate, 4-acetamidobenzoate, and 4-aminosalicylate.

[0085] Salts of the disclosed compounds containing a carboxylic acid or other acidic functional group can be prepared by reacting with a suitable base. Such a pharmaceutically acceptable salt may be made with a base which affords a pharmaceutically acceptable cation, which includes alkali metal salts (especially sodium and potassium), alkaline earth metal salts (especially calcium and magnesium), aluminum salts and ammonium salts, as well as salts made from physiologically acceptable organic bases such as trimethylamine, triethylamine, morpholine, pyridine, piperidine, picoline, dicyclohexylamine, N,N'-dibenzylethylenediamine, 2-hydroxyethylamine, bis-(2-hydroxyethyl)amine, tri-(2-hydroxyethyl)amine, procaine, dibenzylpiperidine, dehydroabietylamine, N,N'-bisdehydroabietylamine, glucamine, N-methylglucamine, collidine, choline, quinine, quinoline, and basic amino acids such as lysine and arginine.

[0086] The invention includes within its scope all possible stoichiometric and non-stoichiometric forms of the salts (e.g., hydrobromide, dihydrobromide, fumarte, hemi-fumarate, etc.) of (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide.

[0087] When a disclosed compound or its salt is named or depicted by structure, it is to be understood that the compound or salt, including solvates (particularly, hydrates) thereof, may exist in crystalline forms, non-crystalline forms or a mixture thereof. The compound or salt, or solvates (particularly, hydrates) thereof, may also exhibit polymorphism (i.e. the capacity to occur in different crystalline forms). These different crystalline forms are typically known as "polymorphs." It is to be understood that the invention includes all polymorphs of any compound of this invention, e.g., all polymorphic forms of any compound named or depicted by structure herein, including any salts and / or solvates (particularly, hydrates) thereof.

[0088] Polymorphs have the same chemical composition but differ in packing, geometrical arrangement, and other descriptive properties of the crystalline solid state. Polymorphs, therefore, may have different physical properties such as shape, density, hardness, deformability, stability, and dissolution properties. Polymorphs typically exhibit different melting points, IR spectra, and X-ray powder diffraction patterns, which may be used for identification. It will be appreciated that different polymorphs may be produced, for example, by changing or adjusting the conditions used in crystallizing / recrystallizing the compound. Polymorphic forms may be characterized and differentiated using a number of conventional analytical techniques, including, but not limited to, X-ray powder diffraction (XRPD) patterns, infrared (IR) spectra, Raman spectra, differential scanning calorimetry (DSC), thermogravimetric analysis (TGA) and solid state nuclear magnetic resonance (SSNMR).

[0089] The skilled artisan will appreciate that pharmaceutically acceptable solvates (particularly, hydrates) of (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide, including pharmaceutically acceptable solvates of a pharmaceutically acceptable salt of (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido )-7-methoxy-1H-benzo[d]imidazole-5-carboxamide, may be formed when solvent molecules are incorporated into the crystalline lattice during crystallization. Solvates may involve nonaqueous solvents such as ethanol, or they may involve water as the solvent that is incorporated into the crystalline lattice. Solvates wherein water is the solvent that is incorporated into the crystalline lattice are typically referred to as "hydrates."

[0090] The present invention includes within its scope all possible stoichiometric and non-stoichiometric salt and / or hydrate forms.

[0091] Salts and solvates (e.g. hydrates and hydrates of salts) of the compounds of the invention which are suitable for use in medicine are those wherein the counterion or associated solvent is pharmaceutically acceptable. Salts having non-pharmaceutically acceptable counterions are within the scope of the present invention, for example, for use as intermediates in the preparation of other compounds of the invention.

[0092] Typically, a pharmaceutically acceptable salt may be readily prepared by using a desired acid or base as appropriate. The resultant salt may crystallize or precipitate from solution, or form by trituration, and may be recovered by filtration, or by evaporation of the solvent.

[0093] Because the compounds of this invention are intended for use in pharmaceutical compositions it will readily be understood that they are each preferably provided in substantially pure form, for example at least 60% pure, more suitably at least 75% pure and preferably at least 85%, especially at least 98% pure (% are on a weight for weight basis). Impure preparations of the compounds may be used for preparing the more pure forms used in the pharmaceutical compositions.

[0094] The invention encompasses all prodrugs of the compounds of this invention, which upon administration to the recipient are capable of providing (directly or indirectly) a compound of this invention, or an active metabolite or residue thereof. Such derivatives are recognisable to those skilled in the art, without undue experimentation. Nevertheless, reference is made to the teaching of Burger's Medicinal Chemistry and Drug Discovery, 5 th< Edition, Vol 1: Principles and Practice, which is incorporated herein by reference to the extent of teaching such derivatives.

[0095] It is to be further understood that the present invention includes within its scope all tautomeric or isomer forms of any free base form of the compounds of this invention as well as all possible stoichiometric and non-stoichiometric salt forms. The compounds of the invention are useful in the treatment or prevention of diseases and disorders in which modulation of STING is beneficial. Such STING mediated diseases and disorders include inflammation, allergic and autoimmune diseases, infectious diseases, cancer and pre-cancerous syndromes. The compounds of the invention are also useful as an immunogenic composition or vaccine adjuvant. Accordingly, this invention is directed to compounds of the invention for use in modulating STING comprising contacting a cell with a compound of the invention.

[0096] One aspect of the invention provides a compounds of the invention for use in the treatment or prevention of STING mediated diseases and disorders, in which agonizing STING is beneficial. Exemplary diseases / disorders include, but are not limited to, cancer, infectious disease (e.g., HIV, HBV, HCV, HPV, and influenza), vaccine adjuvant.

[0097] In one embodiment, this invention provides a compound of the invention for use in therapy. This invention also provides (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof, or a pharmaceutically acceptable salt thereof, or a hydrate thereof for use in therapy. This invention particularly provides (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido )-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide, or a tautomer thereof, or a pharmaceutically acceptable salt thereof, or a hydrate thereof for use in the treatment of a STING-mediated disease or disorder.

[0098] This invention also provides (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide, or a tautomer thereof, or a pharmaceutically acceptable salt thereof, or a hydrate thereof for use as a vaccine adjuvant. There is also therefore provided an immunogenic composition or vaccine adjuvant comprising (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof, or a pharmaceutically acceptable salt thereof, or a hydrate thereof.

[0099] In a further embodiment of the invention, there is provided a composition comprising (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof, or a pharmaceutically acceptable salt thereof, or a hydrate thereof, and one or more immunostimulatory agents.

[0100] In another embodiment, this invention provides a compound of the invention for use in the treatment of a STING-mediated disease or disorder and / or for use as an immunogenic composition or a vaccine adjuvant. In another embodiment, this invention provides (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof, or a pharmaceutically acceptable salt thereof, or a hydrate thereof for use in the amelioration of organ injury or damage sustained as a result of a STING-mediated disease or disorder.

[0101] The disclosure provides for the use of (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof, or a pharmaceutically acceptable salt thereof, or a hydrate thereof, in the manufacture of a vaccine. The disclosure further provides the use of (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof, or a pharmaceutically acceptable salt thereof, or a hydrate thereof for the manufacture of an immunogenic composition comprising an antigen or antigenic composition, for the treatment or prevention of disease. The disclosure further provides the use of (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof, or a pharmaceutically acceptable salt thereof, or a hydrate thereof for the manufacture of a vaccine composition comprising an antigen or antigenic composition, for the treatment or prevention of disease.

[0102] In one embodiment, this invention is directed to (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof, or a pharmaceutically acceptable salt thereof, or a hydrate thereof for use in the treatment of inflammation.

[0103] In one embodiment, this invention is directed (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof, or a pharmaceutically acceptable salt thereof, or a hydrate thereof for use in the treatment of an allergic disease.

[0104] In one embodiment, this invention is directed to (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof, or a pharmaceutically acceptable salt thereof, or a hydrate thereof for use in the treatment of an autoimmune disease.

[0105] In one embodiment, this invention is directed to (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof, or a pharmaceutically acceptable salt thereof, or a hydrate thereof for use in the treatment of an infectious disease.

[0106] In one embodiment, this invention is directed to (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof, or a pharmaceutically acceptable salt thereof, or a hydrate thereof for use in the treatment of an HIV infection in a human. In one embodiment, this invention is directed to (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof, or a pharmaceutically acceptable salt thereof, or a hydrate thereof for use in the treatment of an HIV infection, in a human having or at risk of having the infection. In another embodiment, this invention is directed to (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof, or a pharmaceutically acceptable salt thereof, or a hydrate thereof for use in the treatment of an AIDS infection, in a human.

[0107] In one embodiment, this invention is directed to (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof, or a pharmaceutically acceptable salt thereof, or a hydrate thereof for use in the treatment of an HBV infection in a human. In one embodiment, this invention is directed to (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof, or a pharmaceutically acceptable salt thereof, or a hydrate thereof for use in the treatment of an HCV infection in a human. In one embodiment, this invention is directed to E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof, or a pharmaceutically acceptable salt thereof, or a hydrate thereof for use in the treatment of an HCV infection, in a human having or at risk of having the infection.

[0108] In one embodiment, this invention is directed to (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof, or a pharmaceutically acceptable salt thereof, or a hydrate thereof for use in the treatment of influenza in a human. In one embodiment, this invention is directed to (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof, or a pharmaceutically acceptable salt thereof, or a hydrate thereof for use in the treatment of influenza, in a human having or at risk of having the infection.

[0109] In one embodiment, this invention is directed to (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof, or a pharmaceutically acceptable salt thereof, or a hydrate thereof for use in the treatment of human papillomavirus (HPV) infection in a human. In one embodiment, this invention is directed to (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof, or a pharmaceutically acceptable salt thereof, or a hydrate thereof for use in the treatment of an HPV infection, in a human having or at risk of having the infection.

[0110] As used herein, the terms "cancer," "neoplasm," and "tumor" are used interchangeably and, in either the singular or plural form, refer to cells that have undergone a malignant transformation that makes them pathological to the host organism. Primary cancer cells can be readily distinguished from non-cancerous cells by well-established techniques, particularly histological examination. The definition of a cancer cell, as used herein, includes not only a primary cancer cell, but any cell derived from a cancer cell ancestor. This includes metastasized cancer cells, and in vitro cultures and cell lines derived from cancer cells. When referring to a type of cancer that normally manifests as a solid tumor, a "clinically detectable" tumor is one that is detectable on the basis of tumor mass; e.g., by procedures such as computed tomography (CT) scan, magnetic resonance imaging (MRI), X-ray, ultrasound or palpation on physical examination, and / or which is detectable because of the expression of one or more cancer-specific antigens in a sample obtainable from a patient. Tumors may be a hematopoietic (or hematologic or hematological or blood-related) cancer, for example, cancers derived from blood cells or immune cells, which may be referred to as "liquid tumors." Specific examples of clinical conditions based on hematologic tumors include leukemias such as chronic myelocytic leukemia, acute myelocytic leukemia, chronic lymphocytic leukemia and acute lymphocytic leukemia; plasma cell malignancies such as multiple myeloma, MGUS and Waldenstrom's macroglobulinemia; lymphomas such as non-Hodgkin's lymphoma, Hodgkin's lymphoma; and the like.

[0111] The cancer may be any cancer in which an abnormal number of blast cells or unwanted cell proliferation is present or that is diagnosed as a hematological cancer, including both lymphoid and myeloid malignancies. Myeloid malignancies include, but are not limited to, acute myeloid (or myelocytic or myelogenous or myeloblastic) leukemia (undifferentiated or differentiated), acute promyeloid (or promyelocytic or promyelogenous or promyeloblastic) leukemia, acute myelomonocytic (or myelomonoblastic) leukemia, acute monocytic (or monoblastic) leukemia, erythroleukemia and megakaryocytic (or megakaryoblastic) leukemia. These leukemias may be referred together as acute myeloid (or myelocytic or myelogenous) leukemia (AML). Myeloid malignancies also include myeloproliferative disorders (MPD) which include, but are not limited to, chronic myelogenous (or myeloid) leukemia (CML), chronic myelomonocytic leukemia (CMML), essential thrombocythemia (or thrombocytosis), and polcythemia vera (PCV). Myeloid malignancies also include myelodysplasia (or myelodysplastic syndrome or MDS), which may be referred to as refractory anemia (RA), refractory anemia with excess blasts (RAEB), and refractory anemia with excess blasts in transformation (RAEBT); as well as myelofibrosis (MFS) with or without agnogenic myeloid metaplasia.

[0112] Hematopoietic cancers also include lymphoid malignancies, which may affect the lymph nodes, spleens, bone marrow, peripheral blood, and / or extranodal sites. Lymphoid cancers include B-cell malignancies, which include, but are not limited to, B-cell non-Hodgkin's lymphomas (B-NHLs). B-NHLs may be indolent (or low-grade), intermediate-grade (or aggressive) or high-grade (very aggressive). Indolent Bcell lymphomas include follicular lymphoma (FL); small lymphocytic lymphoma (SLL); marginal zone lymphoma (MZL) including nodal MZL, extranodal MZL, splenic MZL and splenic MZL with villous lymphocytes; lymphoplasmacytic lymphoma (LPL); and mucosa-associated-lymphoid tissue (MALT or extranodal marginal zone) lymphoma. Intermediate-grade B-NHLs include mantle cell lymphoma (MCL) with or without leukemic involvement, diffuse large cell lymphoma (DLBCL), follicular large cell (or grade 3 or grade 3B) lymphoma, and primary mediastinal lymphoma (PML). High-grade B-NHLs include Burkitt's lymphoma (BL), Burkitt-like lymphoma, small non-cleaved cell lymphoma (SNCCL) and lymphoblastic lymphoma. Other B-NHLs include immunoblastic lymphoma (or immunocytoma), primary effusion lymphoma, HIV associated (or AIDS related) lymphomas, and post-transplant lymphoproliferative disorder (PTLD) or lymphoma. B-cell malignancies also include, but are not limited to, chronic lymphocytic leukemia (CLL), prolymphocytic leukemia (PLL), Waldenstrom's macroglobulinemia (WM), hairy cell leukemia (HCL), large granular lymphocyte (LGL) leukemia, acute lymphoid (or lymphocytic or lymphoblastic) leukemia, and Castleman's disease. NHL may also include T-cell non-Hodgkin's lymphoma s(T-NHLs), which include, but are not limited to T-cell non-Hodgkin's lymphoma not otherwise specified (NOS), peripheral T-cell lymphoma (PTCL), anaplastic large cell lymphoma (ALCL), angioimmunoblastic lymphoid disorder (AILD), nasal natural killer (NK) cell / T-cell lymphoma, gamma / delta lymphoma, cutaneous T cell lymphoma, mycosis fungoides, and Sezary syndrome.

[0113] Hematopoietic cancers also include Hodgkin's lymphoma (or disease) including classical Hodgkin's lymphoma, nodular sclerosing Hodgkin's lymphoma, mixed cellularity Hodgkin's lymphoma, lymphocyte predominant (LP) Hodgkin's lymphoma, nodular LP Hodgkin's lymphoma, and lymphocyte depleted Hodgkin's lymphoma. Hematopoietic cancers also include plasma cell diseases or cancers such as multiple myeloma (MM) including smoldering MM, monoclonal gammopathy of undetermined (or unknown or unclear) significance (MGUS), plasmacytoma (bone, extramedullary), lymphoplasmacytic lymphoma (LPL), Waldenstrom's Macroglobulinemia, plasma cell leukemia, and primary amyloidosis (AL). Hematopoietic cancers may also include other cancers of additional hematopoietic cells, including polymorphonuclear leukocytes (or neutrophils), basophils, eosinophils, dendritic cells, platelets, erythrocytes and natural killer cells. Tissues which include hematopoietic cells referred herein to as "hematopoietic cell tissues" include bone marrow; peripheral blood; thymus; and peripheral lymphoid tissues, such as spleen, lymph nodes, lymphoid tissues associated with mucosa (such as the gut-associated lymphoid tissues), tonsils, Peyer's patches and appendix, and lymphoid tissues associated with other mucosa, for example, the bronchial linings.

[0114] In one embodiment, this invention is directed to (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof, or a pharmaceutically acceptable salt thereof, or a hydrate thereof for use in the treatment of cancer and pre-cancerous syndromes.

[0115] Autoimmune diseases associated include, but are not limited to STING associated vasculitis with onset at infancy (SAVI), Aicardi Goutieres syndrome (AGS), chilblain lupus, ataxia telanogiectasia (also referred to as Louis-Bar Syndrome), retinal vasculopathy with cerebral leukodystrophy (RCVL), systemic lupus erythematosus (SLE), cutaneous lupus, lupus nephritis, psoriasis, diabetes mellitus including insulin-dependent diabetes mellitus (IDDM), dermatomyositis, human immunodeficiency virus (HIV), AIDS, polymyositis, systemic sclerosis (scleroderma), and Sjögren's syndrome (SS), rheumatoid arthritis, psoriatic arthritis, polyarthritis, myasthenia gravis, polyarteritis nodosa, vasculitis, cutaneous vasculitis, anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis, Henoch-Schonlein purpura, autoimmune hepatitis, primary sclerosing cholangitis, Wegener's granulomatosis, microscopi polyangiitis, Behcet's disease, spondylitis, giant cell arteritis, polymyalgia rheumatic, Raynaud's phenomenon, primary biliary cirrhosis, primary angiitis of the central nervous system microscopic polyangiitis, neuromyelitis optica and mixed connective tissue disease.

[0116] Inflammation represents a group of vascular, cellular and neurological responses to trauma. Inflammation can be characterized as the movement of inflammatory cells such as monocytes, neutrophils and granulocytes into the tissues. This is usually associated with reduced endothelial barrier function and oedema into the tissues. Inflammation can be classified as either acute or chronic. Acute inflammation is the initial response of the body to harmful stimuli and is achieved by the increased movement of plasma and leukocytes from the blood into the injured tissues. A cascade of biochemical event propagates and matures the inflammatory response, involving the local vascular system, the immune system, and various cells within the injured tissue. Prolonged inflammation, known as chronic inflammation, leads to a progressive shift in the type of cells which are present at the site of inflammation and is characterized by simultaneous destruction and healing of the tissue from the inflammatory process.

[0117] When occurring as part of an immune response to infection or as an acute response to trauma, inflammation can be beneficial and is normally self-limiting. However, inflammation can be detrimental under various conditions. This includes the production of excessive inflammation in response to infectious agents, which can lead to significant organ damage and death (for example, in the setting of sepsis). Moreover, chronic inflammation is generally deleterious and is at the root of numerous chronic diseases, causing severe and irreversible damage to tissues. In such settings, the immune response is often directed against self-tissues (autoimmunity), although chronic responses to foreign entities can also lead to bystander damage to self tissues.

[0118] The aim of anti-inflammatory therapy is therefore to reduce this inflammation, to inhibit autoimmunity when present, and to allow for the physiological process or healing and tissue repair to progress.

[0119] The compounds of this invention may be used to treat inflammation of any tissue and organs of the body, including musculoskeletal inflammation, vascular inflammation, neural inflammation, digestive system inflammation, ocular inflammation, inflammation of the reproductive system, and other inflammation, as exemplified below.

[0120] Musculoskeletal inflammation refers to any inflammatory condition of the musculoskeletal system, particularly those conditions affecting skeletal joints, including joints of the hand, wrist, elbow, shoulder, jaw, spine, neck, hip, knee, ankle, and foot, and conditions affecting tissues connecting muscles to bones such as tendons. Examples of musculoskeletal inflammation which may be treated with compounds of the invention include arthritis (including, for example, osteoarthritis, rheumatoid arthritis, psoriatic arthritis, ankylosing spondylitis, acute and chronic infectious arthritis, arthritis associated with gout and pseudogout, and juvenile idiopathic arthritis), tendonitis, synovitis, tenosynovitis, bursitis, fibrositis (fibromyalgia), epicondylitis, myositis, and osteitis (including, for example, Paget's disease, osteitis pubis, and osteitis fibrosa cystic).

[0121] Ocular inflammation refers to inflammation of any structure of the eye, including the eye lids. Examples of ocular inflammation which may be treated with the compounds of the invention include blepharitis, blepharochalasis, conjunctivitis, dacryoadenitis, keratitis, keratoconjunctivitis sicca (dry eye), scleritis, trichiasis, and uveitis.

[0122] Examples of inflammation of the nervous system which may be treated with the compounds of the invention include encephalitis, Guillain-Barre syndrome, meningitis, neuromyotonia, narcolepsy, multiple sclerosis, myelitis, CNS vasculitis, and schizophrenia.

[0123] Examples of inflammation of the vasculature or lymphatic system which may be treated with the compounds of the invention include arthrosclerosis, arthritis, phlebitis, vasculitis, and lymphangitis.

[0124] Examples of inflammatory conditions of the digestive system which may be treated with the compounds of the invention include cholangitis, cholecystitis, enteritis, enterocolitis, gastritis, gastroenteritis, inflammatory bowel disease (such as Crohn's disease and ulcerative colitis), ileitis, and proctitis.

[0125] Examples of inflammatory conditions of the reproductive system which may be treated with the compounds of the invention include cervicitis, chorioamnionitis, endometritis, epididymitis, omphalitis, oophoritis, orchitis, salpingitis, tubo-ovarian abscess, urethritis, vaginitis, vulvitis, and vulvodynia.

[0126] The compounds of this invention may be used to treat autoimmune conditions having an inflammatory component. Such conditions include acute disseminated alopecia universalise, Behcet's disease, Chagas' disease, STING associated vasculitis with onset at infancy (SAVI), Aicardi Goutieres syndrome (AGS), chilblain lupus, ataxia telangiectasia (also referred to as Louis-Bar Syndrome), retinal vasculopathy with cerebral leukodystrophy (RCVL), ANCA)-associated vasculitis, chronic fatigue syndrome, dysautonomia, encephalomyelitis, ankylosing spondylitis, aplastic anemia, hidradenitis suppurativa, autoimmune hepatitis, autoimmune oophoritis, celiac disease, Crohn's disease, diabetes mellitus type 1, giant cell arteritis, goodpasture's syndrome, Grave's disease, Guillain-Barre syndrome, Hashimoto's disease, Henoch-Schonlein purpura, Kawasaki's disease, lupus erythematosus, microscopic colitis, microscopic polyarteritis, mixed connective tissue disease, multiple sclerosis, myasthenia gravis, opsoclonus myoclonus syndrome, optic neuritis, ord's thyroiditis, pemphigus, polyarteritis nodosa, polymyalgia, rheumatoid arthritis, Reiter's syndrome, Sjogren's syndrome, temporal arteritis, Wegener's granulomatosis, warm autoimmune haemolytic anemia, interstitial cystitis, lyme disease, morphea, psoriasis, sarcoidosis, scleroderma, ulcerative colitis, and vitiligo.

[0127] The compounds of this invention may be used to treat T-cell mediated hypersensitivity diseases having an inflammatory component. Such conditions include contact hypersensitivity, contact dermatitis (including that due to poison ivy), uticaria, skin allergies, respiratory allergies (hayfever, allergic rhinitis) and gluten-sensitive enteropathy (Celiac disease).

[0128] Other inflammatory conditions which may be treated with the compounds of this invention include, for example, appendicitis, dermatitis, dermatomyositis, endocarditis, fibrositis, gingivitis, glossitis, hepatitis, hidradenitis suppurativa, iritis, laryngitis, mastitis, myocarditis, nephritis, otitis, pancreatitis, parotitis, percarditis, peritonitis, pharyngitis, pleuritis, pneumonitis, prostatitis, pyelonephritis, and stomatitis, transplant rejection (involving organs such as kidney, liver, heart, lung, pancreas (e.g., islet cells), bone marrow, cornea, small bowel, skin allografts, skin homografts, and heart valve xenografts, serum sickness, and graft vs host disease), acute pancreatitis, chronic pancreatitis, acute respiratory distress syndrome, Sezary's syndrome, congenital adrenal hyperplasia, nonsuppurative thyroiditis, hypercalcemia associated with cancer, pemphigus, bullous dermatitis herpetiformis, severe erythema multiforme, exfoliative dermatitis, seborrheic dermatitis, seasonal or perennial allergic rhinitis, bronchial asthma, contact dermatitis, atopic dermatitis, drug hypersensitivity reactions, allergic conjunctivitis, keratitis, herpes zoster ophthalmicus, iritis and iridocyclitis, chorioretinitis, optic neuritis, symptomatic sarcoidosis, fulminating or disseminated pulmonary tuberculosis chemotherapy, idiopathic thrombocytopenic purpura in adults, secondary thrombocytopenia in adults, acquired (autoimmune) haemolytic anemia, leukemia and lymphomas in adults, acute leukemia of childhood, regional enteritis, autoimmune vasculitis, multiple sclerosis, chronic obstructive pulmonary disease, solid organ transplant rejection, sepsis. Preferred treatments include treatment of transplant rejection, rheumatoid arthritis, psoriatic arthritis, multiple sclerosis, Type 1 diabetes, asthma, inflammatory bowel disease, systemic lupus erythematosus, psoriasis, chronic pulmonary disease, and inflammation accompanying infectious conditions (e.g., sepsis). In one embodiment, the compounds of this invention may be used to treat asthma.

[0129] Examples of cancer diseases and conditions in which a compounds of this invention may have potentially beneficial antitumor effects include, but are not limited to, cancers of the lung, bone, pancreas, skin, head, neck, uterus, ovaries, stomach, colon, breast, esophagus, small intestine, bowel, endocrine system, thyroid gland, parathyroid gland, adrenal gland, urethra, prostate, penis, testes, ureter, bladder, kidney or liver; rectal cancer; cancer of the anal region; carcinomas of the fallopian tubes, endometrium, cervix, vagina, vulva, renal pelvis, renal cell; sarcoma of soft tissue; myxoma; rhabdomyoma; fibroma; lipoma; teratoma; cholangiocarcinoma; hepatoblastoma; angiosarcoma; hemangioma; hepatoma; fibrosarcoma; chondrosarcoma; myeloma; chronic or acute leukemia; lymphocytic lymphomas; primary CNS lymphoma; neoplasms of the CNS; spinal axis tumours; squamous cell carcinomas; synovial sarcoma; malignant pleural mesotheliomas; brain stem glioma; pituitary adenoma; bronchial adenoma; chondromatous hamartoma; mesothelioma; Hodgkin's Disease or a combination of one or more of the foregoing cancers.

[0130] Suitably the present invention relates to compounds of the invention for use in treating or lessening the severity of cancers selected from the group consisting of brain (gliomas), glioblastomas, astrocytomas, glioblastoma multiforme, Bannayan-Zonana syndrome, Cowden disease, Lhermitte-Duclos disease, Wilm's tumor, Ewing's sarcoma, Rhabdomyosarcoma, ependymoma, medulloblastoma, head and neck, kidney, liver, melanoma, ovarian, pancreatic, adenocarcinoma, ductal adenocarcinoma, adenosquamous carcinoma, acinar cell carcinoma, glucagonoma, insulinoma, prostate, sarcoma, osteosarcoma, giant cell tumor of bone, thyroid, lymphoblastic T cell leukemia, chronic myelogenous leukemia, chronic lymphocytic leukemia, hairy-cell leukemia, acute lymphoblastic leukemia, acute myelogenous leukemia, chronic neutrophilic leukemia, acute lymphoblastic T cell leukemia, plasmacytoma, immunoblastic large cell leukemia, mantle cell leukemia, multiple myeloma, megakaryoblastic leukemia, multiple myeloma, acute megakaryocytic leukemia, promyelocytic leukemia, erythroleukemia, malignant lymphoma, Hodgkin's lymphoma, non-Hodgkin's lymphoma, lymphoblastic T cell lymphoma, Burkitt's lymphoma, follicular lymphoma, neuroblastoma, bladder cancer, urothelial cancer, vulval cancer, cervical cancer, endometrial cancer, renal cancer, mesothelioma, esophageal cancer, salivary gland cancer, hepatocellular cancer, gastric cancer, nasopharangeal cancer, buccal cancer, cancer of the mouth, GIST (gastrointestinal stromal tumor) and testicular cancer. In some embodiments, the compounds of the present invention may be used to treat solid or liquid tumors. In some embodiments, the compounds of the present invention may be used to treat sarcoma, breast cancer, colorectal cancer, gastroesophageal cancer, melanoma, non-small cell lung cancer (NSCLC), clear cell renal cell carcinoma (RCC), lymphomas, squamous cell carcinoma of the head and neck (SCCHN), hepatocellular carcinoma (HCC), and / or Non Hodgkin lymphoma (NHL). Suitably the present invention relates to a method for treating or lessening the severity of pre-cancerous syndromes in a mammal, including a human, wherein the pre-cancerous syndrome is selected from: cervical intraepithelial neoplasia, monoclonal gammopathy of unknown significance (MGUS), myelodysplastic syndrome, aplastic anemia, cervical lesions, skin nevi (pre-melanoma), prostatic intraepithelial (intraductal) neoplasia (PIN), Ductal Carcinoma in situ (DCIS), colon polyps and severe hepatitis or cirrhosis.

[0131] In one aspect the human has a solid tumor. In one aspect the tumor is selected from head and neck cancer, gastric cancer, melanoma, renal cell carcinoma (RCC), esophageal cancer, non-small cell lung carcinoma, prostate cancer, colorectal cancer, ovarian cancer and pancreatic cancer. In one aspect the human has one or more of the following: colorectal cancer (CRC), esophageal, cervical, bladder, breast, head and neck, ovarian, melanoma, renal cell carcinoma (RCC), EC squamous cell, non-small cell lung carcinoma, mesothelioma, and prostate cancer. In another aspect the human has a liquid tumor such as diffuse large B cell lymphoma (DLBCL), multiple myeloma, chronic lymphoblastic leukemia (CLL), follicular lymphoma, acute myeloid leukemia and chronic myelogenous leukemia.

[0132] In one embodiment, the compounds of the present invention may be useful for treatment of skin cancers (e.g., non-melanoma skin cancer, squamous cell carcinoma, basal cell carcinoma) or actinic keratosis. In addition to a field effect for clearing superficial skin cancers, the compounds of the present invention may prevent the development of subsequent skin cancers and pre-malignant actinic keratosis in treated patients.

[0133] The compounds of the present invention may also be useful in the treatment of one or more diseases afflicting mammals which are characterized by cellular proliferation in the area of disorders associated with neo-vascularization and / or vascular permeability including blood vessel proliferative disorders including arthritis (rheumatoid arthritis) and restenosis; fibrotic disorders including hepatic cirrhosis and atherosclerosis; mesangial cell proliferative disorders include glomerulonephritis, diabetic nephropathy, malignant nephrosclerosis, thrombotic microangiopathy syndromes, proliferative retinopathies, organ transplant rejection and glomerulopathies; and metabolic disorders include psoriasis, diabetes mellitus, chronic wound healing, inflammation and neurodegenerative diseases.

[0134] The compounds of this invention may be used to treat neurodegenerative diseases. Exemplary neurodegenerative diseases include, but are not limited to, multiple sclerosis, Huntington's disease, Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis (ALS).

[0135] The compounds of this invention may be used to treat an infectious disease, which is any disease instigated by or coincident with an infection from a pathogen. Pathogens are broadly defined as any species of organism that is foreign to a human tissue environment. Common disease causing pathogens include bacteria (many like TB), viruses (many like HBV, HIV, flu) and parasitic protozoans (like P falciparum that causes malaria). The compounds of this invention may be used to treat infectious diseases derived from bacteria, such as TB infection (Mycobacterium tuberculosis), Chlamydia, Tularemia infection (Francisella tularensis), plasmodium infection or infections from DNA or RNA virus. The compounds of this invention may be used to treat infectious diseases derived from the DNA virus families: Herpesviridae (herpes simplex virus-1, Kaposi's sarcoma-associated virus and Epstein-Barr virus), Papillomaviridae (human papilloma virus), Adenovirus and Hepadnaviridae (Hepatitis B virus). Examples of RNA virus families include Retroviridae (human immunodeficiency virus) Flaviviridae (Dengue virus, Hepatitis C virus), Orthomyxoviridae (influenza), and Coronaviridae (human coronavirus and SARS coronzvirus).

[0136] The compounds of this invention may be employed alone or in combination with other therapeutic agents. As modulators of the immune response, the compounds of this invention may also be used in monotherapy or used in combination with another therapeutic agent in the treatment of diseases and conditions in which modulation of STING is beneficial. Combination therapies according to the present invention thus comprise the administration of (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof, or a pharmaceutically acceptable salt thereof, or a hydrate thereof and at least one other therapeutically active agent. In one embodiment, combination therapies according to the present invention comprise the administration of at least (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof, or a pharmaceutically acceptable salt thereof, or a hydrate thereof and at least one other therapeutic agent. (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide, tautomers thereof, pharmaceutically acceptable salts thereof and hydrates thereof and the other therapeutic agent(s) may be administered together in a single pharmaceutical composition or separately and, when administered separately this may occur simultaneously or sequentially in any order. The amounts of (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof, or a pharmaceutically acceptable salt thereof, or a hydrate thereof, and the other therapeutic agent(s) and the relative timings of administration will be selected in order to achieve the desired combined therapeutic effect. Thus, in a further aspect, there is provided a combination comprising (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof, or a pharmaceutically acceptable salt thereof, or a hydrate thereof, together with one or more other therapeutic agents.

[0137] (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof, or a pharmaceutically acceptable salt thereof, or a hydrate thereof may be used in combination with one or more other therapeutic agents which may be useful in the prevention or treatment of allergic disease, inflammatory disease, or autoimmune disease, for example; antigen immunotherapy, anti-histamines, steroids, NSAIDs, bronchodilators (e.g. beta 2 agonists, adrenergic agonists, anticholinergic agents, theophylline), methotrexate, leukotriene modulators and similar agents; monoclonal antibody therapy such as anti-IgE, anti-TNF, anti-IL-5, anti-IL-6, anti-IL-12, anti-IL-1 and similar agents; receptor therapies e.g. etanercept and similar agents; antigen non-specific immunotherapies (e.g. interferon or other cytokines / chemokines, cytokine / chemokine receptor modulators, cytokine agonists or antagonists, TLR agonists and similar agents).

[0138] (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide, tautomers thereof, pharmaceutically acceptable salts thereof and hydrates thereof may be used in combination with radiotherapy and / or surgery and / or at least one other therapeutic agent which may be useful in the treatment of cancer and pre-cancerous syndromes. Any anti-neoplastic agent that has activity versus a susceptible tumor being treated may be utilized in the combination. Typical anti-neoplastic agents useful include, but are not limited to, anti-microtubule agents such as diterpenoids and vinca alkaloids; platinum coordination complexes; alkylating agents such as nitrogen mustards, oxazaphosphorines, alkylsulfonates, nitrosoureas, and triazenes; antibiotic agents such as anthracyclins, actinomycins and bleomycins; topoisomerase II inhibitors such as epipodophyllotoxins; antimetabolites such as purine and pyrimidine analogues and anti-folate compounds; topoisomerase I inhibitors such as camptothecins; hormones and hormonal analogues; signal transduction pathway inhibitors; non-receptor tyrosine angiogenesis inhibitors; immunotherapeutic agents; proapoptotic agents; cell cycle signaling inhibitors; immuno-oncology agents and immunostimulatory agents.

[0139] Anti-microtubule or anti-mitotic agents are phase specific agents active against the microtubules of tumor cells during M or the mitosis phase of the cell cycle. Examples of anti-microtubule agents include, but are not limited to, diterpenoids and vinca alkaloids.

[0140] Diterpenoids, which are derived from natural sources, are phase specific anti -cancer agents that operate at the G 2 / M phases of the cell cycle. It is believed that the diterpenoids stabilize the β-tubulin subunit of the microtubules, by binding with this protein. Disassembly of the protein appears then to be inhibited with mitosis being arrested and cell death following. Examples of diterpenoids include, but are not limited to, paclitaxel and its analog docetaxel.

[0141] Paclitaxel, 5β,20-epoxy-1,2α,4,7β,10β,13α-hexa-hydroxytax-11-en-9-one 4,10-diacetate 2-benzoate 13-ester with (2R,3S)-N-benzoyl-3-phenylisoserine; is a natural diterpene product isolated from the Pacific yew tree Taxus brevifolia and is commercially available as an injectable solution TAXOL ®< . It is a member of the taxane family of terpenes. Paclitaxel has been approved for clinical use in the treatment of refractory ovarian cancer in the United States (Markman et al., Yale Journal of Biology and Medicine, 64:583, 1991; McGuire et al., Ann. Intem, Med., 111:273, 1989) and for the treatment of breast cancer (Holmes et al., J. Nat. Cancer Inst., 83:1797, 1991.) It is a potential candidate for treatment of neoplasms in the skin (Einzig et. al., Proc. Am. Soc. Clin. Oncol., 20:46) and head and neck carcinomas (Forastire et. al., Sem. Oncol., 20:56, 1990). The compound also shows potential for the treatment of polycystic kidney disease (Woo et. al., Nature, 368:750. 1994), lung cancer and malaria. Treatment of patients with paclitaxel results in bone marrow suppression (multiple cell lineages, Ignoff, R.J. et. al, Cancer Chemotherapy Pocket Guide, 1998) related to the duration of dosing above a threshold concentration (50nM) (Kearns, C.M. et. al., Seminars in Oncology, 3(6) p.16-23, 1995).

[0142] Docetaxel, (2R,3S)- N-carboxy-3-phenylisoserine,N-tert-butyl ester, 13-ester with 5β-20-epoxy-1,2α,4,7β,10β,13α-hexahydroxytax-11-en-9-one 4-acetate 2-benzoate, trihydrate; is commercially available as an injectable solution as TAXOTERE ®< . Docetaxel is indicated for the treatment of breast cancer. Docetaxel is a semisynthetic derivative of paclitaxel q.v., prepared using a natural precursor, 10-deacetyl-baccatin III, extracted from the needle of the European Yew tree.

[0143] Vinca alkaloids are phase specific anti-neoplastic agents derived from the periwinkle plant. Vinca alkaloids act at the M phase (mitosis) of the cell cycle by binding specifically to tubulin. Consequently, the bound tubulin molecule is unable to polymerize into microtubules. Mitosis is believed to be arrested in metaphase with cell death following. Examples of vinca alkaloids include, but are not limited to, vinblastine, vincristine, and vinorelbine.

[0144] Vinblastine, vincaleukoblastine sulfate, is commercially available as VELBAN ®< as an injectable solution. Although, it has possible indication as a second line therapy of various solid tumors, it is primarily indicated in the treatment of testicular cancer and various lymphomas including Hodgkin's Disease; and lymphocytic and histiocytic lymphomas. Myelosuppression is the dose limiting side effect of vinblastine.

[0145] Vincristine, vincaleukoblastine, 22-oxo-, sulfate, is commercially available as ONCOVIN ®< as an injectable solution. Vincristine is indicated for the treatment of acute leukemias and has also found use in treatment regimens for Hodgkin's and non-Hodgkin's malignant lymphomas. Alopecia and neurologic effects are the most common side effect of vincristine and to a lesser extent myelosuppression and gastrointestinal mucositis effects occur.

[0146] Vinorelbine, 3',4'-didehydro -4'-deoxy-C'-norvincaleukoblastine [R-(R ∗< ,R ∗< )-2,3-dihydroxybutanedioate (1:2)(salt)], commercially available as an injectable solution of vinorelbine tartrate (NAVELBINE ®< ), is a semisynthetic vinca alkaloid. Vinorelbine is indicated for use as a single agent or in combination with other chemotherapeutic agents, such as cisplatin, in the treatment of various solid tumors, particularly non-small cell lung, advanced breast, and hormone refractory prostate cancers. Myelosuppression is the most common dose limiting side effect of vinorelbine.

[0147] Platinum coordination complexes are non-phase specific anti-cancer agents, which are interactive with DNA. The platinum complexes enter tumor cells, undergo, aquation and form intra- and interstrand crosslinks with DNA causing adverse biological effects to the tumor. Examples of platinum coordination complexes include, but are not limited to, oxaliplatin, cisplatin and carboplatin.

[0148] Cisplatin, cis-diamminedichloroplatinum, is commercially available as PLATINOL ®< as an injectable solution. Cisplatin is primarily indicated in the treatment of metastatic testicular and ovarian cancer and advanced bladder cancer.

[0149] Carboplatin, platinum, diamine [1,1-cyclobutane-dicarboxylate(2-)-O,O'], is commercially available as PARAPLATIN ®< as an injectable solution. Carboplatin is primarily indicated in the first and second line treatment of advanced ovarian carcinoma.

[0150] Alkylating agents are non-phase anti-cancer specific agents and strong electrophiles. Typically, alkylating agents form covalent linkages, by alkylation, to DNA through nucleophilic moieties of the DNA molecule such as phosphate, amino, sulfhydryl, hydroxy, carboxyl, and imidazole groups. Such alkylation disrupts nucleic acid function leading to cell death. Examples of alkylating agents include, but are not limited to, nitrogen mustards such as cyclophosphamide, melphalan, and chlorambucil; alkyl sulfonates such as busulfan; nitrosoureas such as carmustine; and triazenes such as dacarbazine.

[0151] Cyclophosphamide, 2-[bis(2-chloroethyl)amino]tetrahydro-2H-1,3,2-oxazaphosphorine 2-oxide monohydrate, is commercially available as an injectable solution or tablets as CYTOXAN ®< . Cyclophosphamide is indicated for use as a single agent or in combination with other chemotherapeutic agents, in the treatment of malignant lymphomas, multiple myeloma, and leukemias.

[0152] Melphalan, 4-[bis(2-chloroethyl)amino]-L-phenylalanine, is commercially available as an injectable solution or tablets as ALKERAN ®< . Melphalan is indicated for the palliative treatment of multiple myeloma and non-resectable epithelial carcinoma of the ovary. Bone marrow suppression is the most common dose limiting side effect of melphalan.

[0153] Chlorambucil, 4-[bis(2-chloroethyl)amino]benzenebutanoic acid, is commercially available as LEUKERAN ®< tablets. Chlorambucil is indicated for the palliative treatment of chronic lymphatic leukemia, and malignant lymphomas such as lymphosarcoma, giant follicular lymphoma, and Hodgkin's disease.

[0154] Busulfan, 1,4-butanediol dimethanesulfonate, is commercially available as MYLERAN ®< TABLETS. Busulfan is indicated for the palliative treatment of chronic myelogenous leukemia.

[0155] Carmustine, 1,3-[bis(2-chloroethyl)-1-nitrosourea, is commercially available as single vials of lyophilized material as BiCNU ®< . Carmustine is indicated for palliative treatment as a single agent or in combination with other agents for brain tumors, multiple myeloma, Hodgkin's disease, and non-Hodgkin's lymphomas.

[0156] Dacarbazine, 5-(3,3-dimethyl-1-triazeno)-imidazole-4-carboxamide, is commercially available as single vials of material as DTIC-Dome ®< . Dacarbazine is indicated for the treatment of metastatic malignant melanoma and for use in combination with other agents for the second line treatment of Hodgkin's Disease.

[0157] Antibiotic anti-neoplastics are non-phase specific agents, which bind or intercalate with DNA. Typically, such action results in stable DNA complexes or strand breakage, which disrupts ordinary function of the nucleic acids leading to cell death. Examples of antibiotic anti-neoplastic agents include, but are not limited to, actinomycins such as dactinomycin, anthracyclines such as daunorubicin and doxorubicin; and bleomycins.

[0158] Dactinomycin, also known as Actinomycin D, is commercially available in injectable form as COSMEGEN ®< . Dactinomycin is indicated for the treatment of Wilm's tumor and rhabdomyosarcoma.

[0159] Daunorubicin, (8S-cis-)-8-acetyl-10-[(3-amino-2,3,6-trideoxy-α-L-lyxo-hexopyranosyl)oxy]-7,8,9,10-tetrahydro-6,8,11-trihydroxy-1-methoxy-5,12 naphthacenedione hydrochloride, is commercially available as a liposomal injectable form as DAUNOXOME ®< or as an injectable as CERUBIDINE ®< . Daunorubicin is indicated for remission induction in the treatment of acute nonlymphocytic leukemia and advanced HIV associated Kaposi's sarcoma.

[0160] Doxorubicin, (8S, 10S)-10-[(3-amino-2,3,6-trideoxy-α-L-lyxo-hexopyranosyl)oxy]-8-glycoloyl, 7,8,9,10-tetrahydro-6,8,11-trihydroxy-1-methoxy-5,12 naphthacenedione hydrochloride, is commercially available as an injectable form as RUBEX ®< or ADRIAMYCIN RDF ®< . Doxorubicin is primarily indicated for the treatment of acute lymphoblastic leukemia and acute myeloblastic leukemia, but is also a useful component in the treatment of some solid tumors and lymphomas.

[0161] Bleomycin, a mixture of cytotoxic glycopeptide antibiotics isolated from a strain of Streptomyces verticillus, is commercially available as BLENOXANE ®< . Bleomycin is indicated as a palliative treatment, as a single agent or in combination with other agents, of squamous cell carcinoma, lymphomas, and testicular carcinomas.

[0162] Topoisomerase II inhibitors include, but are not limited to, epipodophyllotoxins. Epipodophyllotoxins are phase specific anti-neoplastic agents derived from the mandrake plant. Epipodophyllotoxins typically affect cells in the S and G 2 phases of the cell cycle by forming a ternary complex with topoisomerase II and DNA causing DNA strand breaks. The strand breaks accumulate and cell death follows. Examples of epipodophyllotoxins include, but are not limited to, etoposide and teniposide.

[0163] Etoposide, 4'-demethyl-epipodophyllotoxin 9[4,6-0-(R)-ethylidene-β-D-glucopyranoside], is commercially available as an injectable solution or capsules as VePESID ®< and is commonly known as VP-16. Etoposide is indicated as a single agent or in combination with other chemotherapy agents in the treatment of testicular and non-small cell lung cancers.

[0164] Teniposide, 4'-demethyl-epipodophyllotoxin 9[4,6-0-(R)-thenylidene-β-D-glucopyranoside], is commercially available as an injectable solution as VUMON ®< and is commonly known as VM-26. Teniposide is indicated as a single agent or in combination with other chemotherapy agents in the treatment of acute leukemia in children.

[0165] Antimetabolite neoplastic agents are phase specific anti-neoplastic agents that act at S phase (DNA synthesis) of the cell cycle by inhibiting DNA synthesis or by inhibiting purine or pyrimidine base synthesis and thereby limiting DNA synthesis. Consequently, S phase does not proceed and cell death follows. Examples of antimetabolite anti-neoplastic agents include, but are not limited to, fluorouracil, methotrexate, cytarabine, mercaptopurine, thioguanine, and gemcitabine.

[0166] 5-Fluorouracil, 5-fluoro-2,4- (1H,3H) pyrimidinedione, is commercially available as fluorouracil. Administration of 5-fluorouracil leads to inhibition of thymidylate synthesis and is also incorporated into both RNA and DNA. The result typically is cell death. 5-Fluorouracil is indicated as a single agent or in combination with other chemotherapy agents in the treatment of carcinomas of the breast, colon, rectum, stomach and pancreas. Other fluoropyrimidine analogs include 5-fluoro deoxyuridine (floxuridine) and 5-fluorodeoxyuridine monophosphate.

[0167] Cytarabine, 4-amino-1-β-D-arabinofuranosyl-2 (1H)-pyrimidinone, is commercially available as CYTOSAR-U ®< and is commonly known as Ara-C. It is believed that cytarabine exhibits cell phase specificity at S-phase by inhibiting DNA chain elongation by terminal incorporation of cytarabine into the growing DNA chain. Cytarabine is indicated as a single agent or in combination with other chemotherapy agents in the treatment of acute leukemia. Other cytidine analogs include 5-azacytidine and 2',2'-difluorodeoxycytidine (gemcitabine).

[0168] Mercaptopurine, 1,7-dihydro-6H-purine-6-thione monohydrate, is commercially available as PURINETHOL ®< . Mercaptopurine exhibits cell phase specificity at S-phase by inhibiting DNA synthesis by an as of yet unspecified mechanism. Mercaptopurine is indicated as a single agent or in combination with other chemotherapy agents in the treatment of acute leukemia. A useful mercaptopurine analog is azathioprine.

[0169] Thioguanine, 2-amino-1,7-dihydro-6H-purine-6-thione, is commercially available as TABLOID ®< . Thioguanine exhibits cell phase specificity at S-phase by inhibiting DNA synthesis by an as of yet unspecified mechanism. Thioguanine is indicated as a single agent or in combination with other chemotherapy agents in the treatment of acute leukemia. Other purine analogs include pentostatin, erythrohydroxynonyladenine (EHNA), fludarabine phosphate, and cladribine.

[0170] Gemcitabine, 2'-deoxy-2', 2'-difluorocytidine monohydrochloride (β-isomer), is commercially available as GEMZAR ®< . Gemcitabine exhibits cell phase specificity at S-phase and by blocking progression of cells through the G1 / S boundary. Gemcitabine is indicated in combination with cisplatin in the treatment of locally advanced non-small cell lung cancer and alone in the treatment of locally advanced pancreatic cancer.

[0171] Methotrexate, N-[4[[(2,4-diamino-6-pteridinyl) methyl]methylamino] benzoyl]-L-glutamic acid, is commercially available as methotrexate sodium. Methotrexate exhibits cell phase effects specifically at S-phase by inhibiting DNA synthesis, repair and / or replication through the inhibition of dihydrofolic acid reductase which is required for synthesis of purine nucleotides and thymidylate. Methotrexate is indicated as a single agent or in combination with other chemotherapy agents in the treatment of choriocarcinoma, meningeal leukemia, non-Hodgkin's lymphoma, and carcinomas of the breast, head, neck, ovary and bladder.

[0172] Camptothecins, including, camptothecin and camptothecin derivatives are available or under development as Topoisomerase I inhibitors. Camptothecins cytotoxic activity is believed to be related to its Topoisomerase I inhibitory activity. Examples of camptothecins include, but are not limited to irinotecan, topotecan, and the various optical forms of 7-(4-methylpiperazino-methylene)-10, 11-ethylenedioxy-20-camptothecin described below.

[0173] Irinotecan HCI, (4S)-4,11-diethyI-4-hydroxy-9-[(4-piperidinopiperidino) carbonyloxy]-1H-pyrano[3',4',6,7]indolizino[1,2-b]quinoline-3,14(4H,12H)-dione hydrochloride, is commercially available as the injectable solution CAMPTOSAR ®< . Irinotecan is a derivative of camptothecin which binds, along with its active metabolite SN-38, to the topoisomerase I - DNA complex. It is believed that cytotoxicity occurs as a result of irreparable double strand breaks caused by interaction of the topoisomerase I: DNA: irinotecan or SN-38 ternary complex with replication enzymes. Irinotecan is indicated for treatment of metastatic cancer of the colon or rectum.

[0174] Topotecan HCl, (S)-10-[(dimethylamino)methyl]-4-ethyl-4,9-dihydroxy-1H-pyrano[3';4',6,7]indolizino[1,2-b]quinoline-3,14-(4H,12H)-dione monohydrochloride, is commercially available as the injectable solution HYCAMTIN ®< . Topotecan is a derivative of camptothecin which binds to the topoisomerase I - DNA complex and prevents relegation of single strand breaks caused by Topoisomerase I in response to torsional strain of the DNA molecule. Topotecan is indicated for second line treatment of metastatic carcinoma of the ovary and small cell lung cancer.

[0175] Hormones and hormonal analogues are useful compounds for treating cancers in which there is a relationship between the hormone(s) and growth and / or lack of growth of the cancer. Examples of hormones and hormonal analogues useful in cancer treatment include, but are not limited to, adrenocorticosteroids such as prednisone and prednisolone which are useful in the treatment of malignant lymphoma and acute leukemia in children ; aminoglutethimide and other aromatase inhibitors such as anastrozole, letrozole, vorozole, and exemestane useful in the treatment of adrenocortical carcinoma and hormone dependent breast carcinoma containing estrogen receptors; progestins such as megestrol acetate useful in the treatment of hormone dependent breast cancer and endometrial carcinoma; estrogens, and anti-estrogens such as fulvestrant, flutamide, nilutamide, bicalutamide, cyproterone acetate and 5α-reductases such as finasteride and dutasteride, useful in the treatment of prostatic carcinoma and benign prostatic hypertrophy; anti-estrogens such as tamoxifen, toremifene, raloxifene, droloxifene, iodoxyfene, as well as selective estrogen receptor modulators (SERMS) such those described in U.S. Patent Nos. 5,681,835, 5,877,219, and 6,207,716, useful in the treatment of hormone dependent breast carcinoma and other susceptible cancers; and gonadotropin-releasing hormone (GnRH) and analogues thereof which stimulate the release of leutinizing hormone (LH) and / or follicle stimulating hormone (FSH) for the treatment prostatic carcinoma, for instance, LHRH agonists and antagonists such as goserelin acetate and luprolide.

[0176] Signal transduction pathway inhibitors are those inhibitors, which block or inhibit a chemical process which evokes an intracellular change. As used herein this change is cell proliferation or differentiation. Signal transduction inhibitors useful in the present invention include inhibitors of receptor tyrosine kinases, non-receptor tyrosine kinases, SH2 / SH3domain blockers, serine / threonine kinases, phosphotidyl inositol-3 kinases, myo-inositol signaling, and Ras oncogenes.

[0177] Several protein tyrosine kinases catalyze the phosphorylation of specific tyrosyl residues in various proteins involved in the regulation of cell growth. Such protein tyrosine kinases can be broadly classified as receptor or non-receptor kinases.

[0178] Receptor tyrosine kinases are transmembrane proteins having an extracellular ligand binding domain, a transmembrane domain, and a tyrosine kinase domain. Receptor tyrosine kinases are involved in the regulation of cell growth and are generally termed growth factor receptors. Inappropriate or uncontrolled activation of many of these kinases, i.e. aberrant kinase growth factor receptor activity, for example by over-expression or mutation, has been shown to result in uncontrolled cell growth. Accordingly, the aberrant activity of such kinases has been linked to malignant tissue growth. Consequently, inhibitors of such kinases could provide cancer treatment methods. Growth factor receptors include, for example, epidermal growth factor receptor (EGFr), platelet derived growth factor receptor (PDGFr), erbB2, erbB4, ret, vascular endothelial growth factor receptor (VEGFr), tyrosine kinase with immunoglobulin-like and epidermal growth factor homology domains (TIE-2), insulin growth factor -I (IGFI) receptor, macrophage colony stimulating factor (cfms), BTK, ckit, cmet, fibroblast growth factor (FGF) receptors, Trk receptors (TrkA, TrkB, and TrkC), ephrin (eph) receptors, and the RET protooncogene. Several inhibitors of growth receptors are under development and include ligand antagonists, antibodies, tyrosine kinase inhibitors and anti-sense oligonucleotides. Growth factor receptors and agents that inhibit growth factor receptor function are described, for instance, in Kath, John C., Exp. Opin. Ther. Patents (2000) 10(6):803-818; Shawver et al DDT Vol 2, No. 2 February 1997; and Lofts, F. J. et al, "Growth factor receptors as targets", New Molecular Targets for Cancer Chemotherapy, ed. Workman, Paul and Kerr, David, CRC press 1994, London.

[0179] Tyrosine kinases, which are not growth factor receptor kinases are termed non-receptor tyrosine kinases. Non-receptor tyrosine kinases useful in the present invention, which are targets or potential targets of anti-cancer drugs, include cSrc, Lck, Fyn, Yes, Jak, cAbl, FAK (Focal adhesion kinase), Brutons tyrosine kinase, and Bcr-Abl. Such non-receptor kinases and agents which inhibit non-receptor tyrosine kinase function are described in Sinh, S. and Corey, S.J., (1999) Journal of Hematotherapy and Stem Cell Research 8 (5): 465 - 80; and Bolen, J.B., Brugge, J.S., (1997) Annual review of Immunology. 15: 371-404.

[0180] SH2 / SH3 domain blockers are agents that disrupt SH2 or SH3 domain binding in a variety of enzymes or adaptor proteins including, PI3-K p85 subunit, Src family kinases, adaptor molecules (Shc, Crk, Nck, Grb2) and Ras-GAP. SH2 / SH3 domains as targets for anti-cancer drugs are discussed in Smithgall, T.E. (1995), Journal of Pharmacological and Toxicological Methods. 34(3) 125-32.

[0181] Inhibitors of Serine / Threonine Kinases including MAP kinase cascade blockers which include blockers of Raf kinases (rafk), Mitogen or Extracellular Regulated Kinase (MEKs), and Extracellular Regulated Kinases (ERKs); and Protein kinase C family member blockers including blockers of PKCs (alpha, beta, gamma, epsilon, mu, lambda, iota, zeta). IkB kinase family (IKKa, IKKb), PKB family kinases, akt kinase family members, and TGF beta receptor kinases. Such Serine / Threonine kinases and inhibitors thereof are described in Yamamoto, T., Taya, S., Kaibuchi, K., (1999), Journal of Biochemistry. 126 (5) 799-803; Brodt, P, Samani, A., and Navab, R. (2000), Biochemical Pharmacology, 60. 1101-1107; Massague, J., Weis-Garcia, F. (1996) Cancer Surveys. 27:41-64; Philip, P.A., and Harris, A.L. (1995), Cancer Treatment and Research. 78: 3-27, Lackey, K. et al Bioorganic and Medicinal Chemistry Letters, (10), 2000, 223-226; U.S. Patent No. 6,268,391; and Martinez-Iacaci, L., et al, Int. J. Cancer (2000), 88(1), 44-52.

[0182] Inhibitors of Phosphotidyl inositol-3 Kinase family members including blockers of PI3-kinase, ATM, DNA-PK, and Ku are also useful in the present invention. Such kinases are discussed in Abraham, R.T. (1996), Current Opinion in Immunology. 8 (3) 412-8; Canman, C.E., Lim, D.S. (1998), Oncogene 17 (25) 3301-3308; Jackson, S.P. (1997), International Journal of Biochemistry and Cell Biology. 29 (7):935-8; and Zhong, H. et al, Cancer res, (2000) 60(6), 1541-1545.

[0183] Also useful in the present invention are Myo-inositol signaling inhibitors such as phospholipase C blockers and Myoinositol analogues. Such signal inhibitors are described in Powis, G., and Kozikowski A., (1994) New Molecular Targets for Cancer Chemotherapy ed., Paul Workman and David Kerr, CRC press 1994, London.

[0184] Another group of signal transduction pathway inhibitors are inhibitors of Ras Oncogene. Such inhibitors include inhibitors of farnesyltransferase, geranyl-geranyl transferase, and CAAX proteases as well as anti-sense oligonucleotides, ribozymes and immunotherapy. Such inhibitors have been shown to block ras activation in cells containing wild type mutant ras, thereby acting as antiproliferation agents. Ras oncogene inhibition is discussed in Scharovsky, O.G., Rozados, V.R., Gervasoni, S.I. Matar, P. (2000), Journal of Biomedical Science. 7(4) 292-8; Ashby, M.N. (1998), Current Opinion in Lipidology. 9 (2) 99 - 102; and BioChim. Biophys. Acta, (19899) 1423(3): 19-30.

[0185] As mentioned above, antibody antagonists to receptor kinase ligand binding may also serve as signal transduction inhibitors. This group of signal transduction pathway inhibitors includes the use of humanized antibodies to the extracellular ligand binding domain of receptor tyrosine kinases. For example Imclone C225 EGFR specific antibody (see Green, M.C. et al, Monoclonal Antibody Therapy for Solid Tumors, Cancer Treat. Rev., (2000), 26(4), 269-286); Herceptin ®< erbB2 antibody (see Tyrosine Kinase Signaling in Breast cancer:erbB Family Receptor Tyrosine Kinases, Breast cancer Res., 2000, 2(3), 176-183); and 2CB VEGFR2 specific antibody (see Brekken, R.A. et al, Selective Inhibition of VEGFR2 Activity by a monoclonal Anti-VEGF antibody blocks tumor growth in mice, Cancer Res. (2000) 60, 5117-5124).

[0186] Anti-angiogenic therapeutic agents including non-receptor MEK angiogenesis inhibitors may also be useful. Anti-angiogenic agents such as those which inhibit the effects of vascular endothelial growth factor, (for example the anti-vascular endothelial cell growth factor antibody bevacizumab [Avastin ™< ], and compounds that work by other mechanisms (for example linomide, inhibitors of integrin αvβ3 function, endostatin and angiostatin).

[0187] Agents used in immunotherapeutic regimens may also be useful in combination with the compounds of the invention. Immunotherapy approaches, including for example ex-vivo and in-vivo approaches to increase the immunogenecity of patient tumor cells, such as transfection with cytokines such as interleukin 2, interleukin 4 or granulocyte-macrophage colony stimulating factor, approaches to decrease T-cell energy, approaches using transfected immune cells such as cytokine-transfected dendritic cells, approaches using cytokine-transfected tumor cell lines and approaches using anti-idiotypic antibodies.

[0188] Therapeutic agents used in proapoptotic regimens (e.g., bcl-2 antisense oligonucleotides) may also be used in the combination of the present invention.

[0189] Cell cycle signaling inhibitors inhibit molecules involved in the control of the cell cycle. A family of protein kinases called cyclin dependent kinases (CDKs) and their interaction with a family of proteins termed cyclins controls progression through the eukaryotic cell cycle. The coordinate activation and inactivation of different cyclin / CDK complexes is necessary for normal progression through the cell cycle. Several inhibitors of cell cycle signaling are under development. For instance, examples of cyclin dependent kinases, including CDK2, CDK4, and CDK6 and inhibitors for the same are described in, for instance, Rosania et al, Exp. Opin. Ther. Patents (2000) 10(2):215-230.

[0190] In one embodiment, the combination of the present invention comprises a compound of the inventionand at least one anti-neoplastic agent selected from anti-microtubule agents, platinum coordination complexes, alkylating agents, antibiotic agents, topoisomerase II inhibitors, antimetabolites, topoisomerase I inhibitors, hormones and hormonal analogues, signal transduction pathway inhibitors, non-receptor tyrosine MEK angiogenesis inhibitors, immunotherapeutic agents, proapoptotic agents, and cell cycle signaling inhibitors.

[0191] In one embodiment, the combination of the present invention comprises a compound of the invention, and at least one anti-neoplastic agent which is an anti-microtubule agent selected from diterpenoids and vinca alkaloids.

[0192] In a further embodiment, at least one anti-neoplastic agent is a diterpenoid. In a further embodiment, at least one anti-neoplastic agent is a vinca alkaloid.

[0193] In one embodiment, the combination of the present invention comprises a compound of the invention , and at least one anti-neoplastic agent, which is a platinum coordination complex.

[0194] In a further embodiment, at least one anti-neoplastic agent is paclitaxel, carboplatin, or vinorelbine. In a further embodiment, at least one anti-neoplastic agent is carboplatin. In a further embodiment, at least one anti-neoplastic agent is vinorelbine. In a further embodiment, at least one anti-neoplastic agent is paclitaxel. In one embodiment, the combination of the present invention comprises a compound of the invention, and at least one anti-neoplastic agent which is a signal transduction pathway inhibitor.

[0195] In a further embodiment, the signal transduction pathway inhibitor is an inhibitor of a growth factor receptor kinase VEGFR2, TIE2, PDGFR, BTK, erbB2, EGFr, IGFR-1, TrkA, TrkB, TrkC, or c-fms. In a further embodiment, the signal transduction pathway inhibitor is an inhibitor of a serine / threonine kinase rafk, akt, or PKC-zeta. In a further embodiment, the signal transduction pathway inhibitor is an inhibitor of a non- receptor tyrosine kinase selected from the src family of kinases. In a further embodiment, the signal transduction pathway inhibitor is an inhibitor of c-src. In a further embodiment, the signal transduction pathway inhibitor is an inhibitor of Ras oncogene selected from inhibitors of farnesyl transferase and geranylgeranyl transferase. In a further embodiment, the signal transduction pathway inhibitor is an inhibitor of a serine / threonine kinase selected from the group consisting of PI3K.

[0196] In a further embodiment, the signal transduction pathway inhibitor is a dual EGFr / erbB2 inhibitor, for example N-{3-chloro-4-[(3-fluorobenzyl) oxy]phenyl}-6-[5-({[2-(methanesulphonyl) ethyl]amino}methyl)-2-furyl]-4-quinazolinamine.

[0197] In one embodiment, the combination of the present invention comprises a compound of the invention, and at least one anti-neoplastic agent which is a cell cycle signaling inhibitor. In further embodiment, cell cycle signaling inhibitor is an inhibitor of CDK2, CDK4 or CDK6.

[0198] Additional examples of other therapeutic agents (e.g., anti-neoplastic agent) for use in combination or co-administered with a compound of the invention are immuno-modulators.

[0199] As used herein "immuno-modulators" refer to any substance including monoclonal antibodies that affects the immune system. Immuno-modulators can be used as anti-neoplastic agents for the treatment of cancer. For example, immune-modulators include, but are not limited to, anti-CTLA-4 antibodies such as ipilimumab (YERVOY) and anti-PD-1 antibodies (Opdivo / nivolumab and Keytruda / pembrolizumab). Other immuno-modulators include, but are not limited to, ICOS antibodies, OX-40 antibodies, PD-L1 antibodies, LAG3 antibodies, TIM-3 antibodies, 41BB antibodies and GITR antibodies.

[0200] Additional examples of other therapeutic agents (anti-neoplastic agent) for use in combination or co-administered with a compound of this invention are anti-PD-Ll agents. Anti-PD-Ll antibodies and methods of making the same are known in the art. Such antibodies to PD-L1 may be polyclonal or monoclonal, and / or recombinant, and / or humanized. Exemplary PD-L1 antibodies are disclosed in US Patent Nos. 8,217,149, 8,383,796, 8,552,154, 9,212,224, and 8,779,108, and US Patent Appln. Pub. Nos. 20110280877, 2014 / 0341902 and 20130045201. Additional exemplary antibodies to PD-L1 (also referred to as CD274 or B7-H1) and methods for use are disclosed in US Patent Nos. 7,943,743, 8,168,179; and 7,595,048 WO2014055897, WO2016007235 and US Patent Appln. Pub. Nos. 20130034559, 20130034559 and 20150274835. PD-L1 antibodies are in development as immuno-modulatory agents or immuno-modulator for the treatment of cancer.

[0201] In one embodiment, the antibody to PD-L1 is an antibody disclosed in US Patent No. 8,217,149. In another embodiment, the anti-PD-Ll antibody comprises the CDRs of an antibody disclosed in US Patent No. 8,217,149. In another embodiment, the antibody to PD-L1 is an antibody disclosed in US Patent No. 8,779,108. In another embodiment, the anti-PD-L1 antibody comprises the CDRs of an antibody disclosed in US Application No. 8,779,108. In another embodiment, the antibody to PD-L1 is an antibody disclosed in US Patent Appln. Pub. No. 20130045201. In another embodiment, the anti-PD-Ll antibody comprises the CDRs of an antibody disclosed in US Patent Appln. Pub. No. 20130045201. In one embodiment, the anti-PD-Ll antibody is BMS-936559 (MDX-1105), which was described in WO 2007 / 005874. In another embodiment, the anti-PD-Ll antibody is MPDL3280A (RG7446). In another embodiment, the anti-PD-Ll antibody is MEDI4736, which is an anti-PD-L1 monoclonal antibody described in WO 2011 / 066389 and US 2013 / 034559. In another embodiment, the anti-PD-Ll antibody is TECENTRIQ ™< (atezolizumab), which is an anti-PDL1 cancer immunotherapy which was approved in the US in May 2016 for specific types of bladder cancer. In another embodiment, anti-PD-Ll antibody is YW243.55.S70 which is an anti-PD-Ll described in WO 2010 / 077634 and U.S. Pat. No. 8,217,149. Examples of anti-PD-L1 antibodies useful for the methods of this invention, and methods for making thereof are described in PCT patent application WO 2010 / 077634, WO 2007 / 005874, WO 2011 / 066389, U.S. Pat. No. 8,217,149, and US 2013 / 034559.

[0202] Other examples of mAbs that bind to human PD-L1, and useful in the treatment method, medicaments and uses of the present invention, are described in WO2013 / 019906, WO2010 / 077634 A1 and US8383796. Specific anti-human PD-L1 mAbs useful as the PD-1 antagonist in the treatment method, medicaments and uses of the present invention include MPDL3280A, BMS-936559, MEDI4736, MSB0010718C.

[0203] Additional examples of other therapeutic agents (anti-neoplastic agent) for use in combination or co-administered with a compound of this invention are PD-1 antagonist.

[0204] "PD-1 antagonist" means any chemical compound or biological molecule that blocks binding of PD-L1 expressed on a cancer cell to PD-1 expressed on an immune cell (T cell, B cell or NKT cell) and preferably also blocks binding of PD-L2 expressed on a cancer cell to the immune-cell expressed PD-1. Alternative names or synonyms for PD-1 and its ligands include: PDCD1, PD1, CD279 and SLEB2 for PD-1; PDCD1L1, PDL1, B7H1, B7-4, CD274 and B7-H for PD-L1; and PDCD1L2, PDL2, B7-DC, Btdc and CD273 for PD-L2. In any embodiments of the aspects or embodiments of the present invention in which a human individual is to be treated, the PD-1 antagonist blocks binding of human PD-L1 to human PD-1, and preferably blocks binding of both human PD-L1 and PD-L2 to human PD-1. Human PD-1 amino acid sequences can be found in NCBI Locus No.: NP_005009. Human PD-L1 and PD-L2 amino acid sequences can be found in NCBI Locus No.: NP_054862 and NP_079515, respectively.

[0205] PD-1 antagonists useful in any of the aspects of the present invention include a monoclonal antibody (mAb), or antigen binding fragment thereof, which specifically binds to PD-1 or PD-L1, and preferably specifically binds to human PD-1 or human PD-L1. The mAb may be a human antibody, a humanized antibody or a chimeric antibody, and may include a human constant region. In some embodiments, the human constant region is selected from the group consisting of IgG1, IgG2, IgG3 and IgG4 constant regions, and in preferred embodiments, the human constant region is an IgG1 or IgG4 constant region. In some embodiments, the antigen binding fragment is selected from the group consisting of Fab, Fab'-SH, F(ab') 2 , scFv and Fv fragments.

[0206] Examples of mAbs that bind to human PD-1, and useful in the various aspects and embodiments of the present invention, are described in US7488802, US7521051, US8008449, US8354509, US8168757, WO2004 / 004771, WO2004 / 072286, WO2004 / 056875, and US2011 / 0271358.

[0207] Specific anti-human PD-1 mAbs useful as the PD-1 antagonist in any of the aspects and embodiments of the present invention include: MK-3475, a humanized IgG4 mAb with the structure described in WHO Drug Information, Vol. 27, No. 2, pages 161-162 (2013) and which comprises the heavy and light chain amino acid sequences shown in Figure 6; nivolumab, a human IgG4 mAb with the structure described in WHO Drug Information, Vol. 27, No. 1, pages 68-69 (2013) and which comprises the heavy and light chain amino acid sequences shown in Figure 7; the humanized antibodies h409A11, h409A16 and h409A17, which are described in WO2008 / 156712, and AMP-514, which is being developed by Medimmune.

[0208] Other PD-1 antagonists useful in the any of the aspects and embodiments of the present invention include an immunoadhesin that specifically binds to PD-1, and preferably specifically binds to human PD-1, e.g., a fusion protein containing the extracellular or PD-1 binding portion of PD-L1 or PD-L2 fused to a constant region such as an Fc region of an immunoglobulin molecule. Examples of immunoadhesion molecules that specifically bind to PD-1 are described in WO2010 / 027827 and WO2011 / 066342. Specific fusion proteins useful as the PD-1 antagonist in the treatment method, medicaments and uses of the present invention include AMP-224 (also known as B7-DCIg), which is a PD-L2-FC fusion protein and binds to human PD-1.

[0209] KEYTRUDA / pembrolizumab is an anti-PD-1 antibody marketed for the treatment of lung cancer by Merck. The amino acid sequence of pembrolizumab and methods of using are disclosed in US Patent No. 8,168,757.

[0210] Opdivo / nivolumab is a fully human monoclonal antibody marketed by Bristol Myers Squibb directed against the negative immunoregulatory human cell surface receptor PD-1 (programmed death-1 or programmed cell death-1 / PCD-1) with immunopotentiation activity. Nivolumab binds to and blocks the activation of PD-1, an Ig superfamily transmembrane protein, by its ligands PD-L1 and PD-L2, resulting in the activation of T-cells and cell-mediated immune responses against tumor cells or pathogens. Activated PD-1 negatively regulates T-cell activation and effector function through the suppression of PI3K / Akt pathway activation. Other names for nivolumab include: BMS-936558, MDX-1106, and ONO-4538. The amino acid sequence for nivolumab and methods of using and making are disclosed in US Patent No. US 8,008,449.

[0211] Additional examples of other therapeutic agents (anti-neoplastic agent) for use in combination or co-administered with a compound of the invention are antibodies to ICOS.

[0212] ICOS is a co-stimulatory T cell receptor with structural and functional relation to the CD28 / CTLA-4-Ig superfamily (Hutloff, et al., "ICOS is an inducible T-cell co-stimulator structurally and functionally related to CD28", Nature, 397: 263-266 (1999)). Activation of ICOS occurs through binding by ICOS-L (B7RP-1 / B7-H2). Neither B7-1 nor B7-2 (ligands for CD28 and CTLA4) bind or activate ICOS. However, ICOS-L has been shown to bind weakly to both CD28 and CTLA-4 (Yao S et al., "B7-H2 is a costimulatory ligand for CD28 in human", Immunity, 34(5); 729-40 (2011)). Expression of ICOS appears to be restricted to T cells. ICOS expression levels vary between different T cell subsets and on T cell activation status. ICOS expression has been shown on resting TH17, T follicular helper (TFH) and regulatory T (Treg) cells; however, unlike CD28; it is not highly expressed on naïve T H 1 and T H 2 effector T cell populations (Paulos CM et al., "The inducible costimulator (ICOS) is critical for the development of human Th17 cells", Sci Transl Med, 2(55); 55ra78 (2010)). ICOS expression is highly induced on CD4+ and CD8+ effector T cells following activation through TCR engagement (Wakamatsu E, et al., "Convergent and divergent effects of costimulatory molecules in conventional and regulatory CD4+ T cells", Proc Natal Acad Sci USA, 110(3); 1023-8 (2013)).

[0213] CDRs for murine antibodies to human ICOS having agonist activity are shown in PCT / EP2012 / 055735 (WO 2012 / 131004). Antibodies to ICOS are also disclosed in WO 2008 / 137915, WO 2010 / 056804, EP 1374902, EP1374901, and EP1125585.

[0214] Agonist antibodies to ICOS or ICOS binding proteins are disclosed in WO2012 / 13004, WO 2014 / 033327, WO2016 / 120789, US20160215059, and US20160304610. In one embodiment, agonist antibodies to ICOS include ICOS binding proteins or antigen binding portions thereof comprising one or more of: CDRH1 as set forth in SEQ ID NO:1; CDRH2 as set forth in SEQ ID NO:2; CDRH3 as set forth in SEQ ID NO:3; CDRL1 as set forth in SEQ ID NO:4; CDRL2 as set forth in SEQ ID NO:5 and / or CDRL3 as set forth in SEQ ID NO:6 or a direct equivalent of each CDR wherein a direct equivalent has no more than two amino acid substitutions in said CDR as disclosed in WO2016 / 120789, which is incorporated by reference in its entirety herein. In one embodiment, the ICOS binding protein or antigen binding portion thereof is an agonist antibody to ICOS comprising a V H domain comprising an amino acid sequence at least 90% identical to the amino acid sequence set forth in SEQ ID NO:7 and / or a V L domain comprising an amino acid sequence at least 90% identical to the amino acid sequence as set forth in SEQ ID NO:8 as set forth in WO2016 / 120789 wherein said ICOS binding protein specifically binds to human ICOS. In one embodiment, the ICOS binding protein is an agonist antibody to ICOS comprising a V H domain comprising the amino acid sequence set forth in SEQ ID NO:7 and a V L domain comprising the amino acid sequence set forth in SEQ ID NO:8 as set forth in WO2016 / 120789.

[0215] Yervoy (ipilimumab) is a fully human CTLA-4 antibody marketed by Bristol Myers Squibb. The protein structure of ipilimumab and methods are using are described in US Patent Nos. 6,984,720 and 7,605,238.

[0216] CD134, also known as OX40, is a member of the TNFR-superfamily of receptors which is not constitutively expressed on resting naïve T cells, unlike CD28. OX40 is a secondary costimulatory molecule, expressed after 24 to 72 hours following activation; its ligand, OX40L, is also not expressed on resting antigen presenting cells, but is following their activation. Expression of OX40 is dependent on full activation of the T cell; without CD28, expression of OX40 is delayed and of fourfold lower levels. OX-40 antibodies, OX-40 fusion proteins and methods of using them are disclosed in US Patent Nos: US 7,504,101; US 7,758,852; US 7,858,765; US 7,550,140; US 7,960,515; WO2012027328; WO2013028231.

[0217] In one embodiment, the OX40 antigen binding protein is one disclosed in WO2012 / 027328 (PCT / US2011 / 048752), international filing date 23 August 2011. In another embodiment, the antigen binding protein comprises the CDRs of an antibody disclosed in WO2012 / 027328 (PCT / US2011 / 048752), international filing date 23 August 2011, or CDRs with 90% identity to the disclosed CDR sequences. In a further embodiment the antigen binding protein comprises a VH, a VL, or both of an antibody disclosed in WO2012 / 027328 (PCT / US2011 / 048752), international filing date 23 August 2011, or a VH or a VL with 90% identity to the disclosed VH or VL sequences.

[0218] In another embodiment, the OX40 antigen binding protein is disclosed in WO2013 / 028231 (PCT / US2012 / 024570), international filing date 9 Feb. 2012, which is incorporated by reference in its entirety herein. In another embodiment, the antigen binding protein comprises the CDRs of an antibody disclosed in WO2013 / 028231 (PCT / US2012 / 024570), international filing date 9 Feb. 2012, or CDRs with 90% identity to the disclosed CDR sequences. In a further embodiment, the antigen binding protein comprises a VH, a VL, or both of an antibody disclosed in WO2013 / 028231 (PCT / US2012 / 024570), international filing date 9 Feb. 2012, or a VH or a VL with 90% identity to the disclosed VH or VL sequences. In one embodiment, the OX40 antigen binding protein is an isolated agonist antibody to OX40 comprising a light chain variable region having a sequence at least 90% identical to the amino acid sequence of SEQ ID NO:10 as set forth in WO2013 / 028231 and a heavy chain variable region having a sequence at least 90% identical to the amino acid sequence of SEQ ID NO:4 as set forth in WO2013 / 028231. In one embodiment, the OX40 antigen binding protein is an isolated antibody comprising a light chain variable comprising the amino acid sequence of SEQ ID NO:10 as set forth in WO2013 / 028231 and a heavy chain variable region comprising the amino acid sequence of SEQ ID NO:4 as set forth in WO2013 / 028231.

[0219] Thus, in one embodiment there is provided (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof, or a pharmaceutically acceptable salt thereof, or a hydrate thereof with at least one immuno-modulator for the treatment of a human in need thereof. In one embodiment, the immuno-modulator is selected from an ICOS agonist antibody, an OX-40 antibody or a PD-1 antibody. In one embodiment, the human has cancer.

[0220] Additional examples of other therapeutic agents for use in combination or co-administered with (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof, or a pharmaceutically acceptable salt thereof, or a hydrate thereof are immunostimulatory agents.

[0221] As used herein "immunostimulatory agent" refers to any agent that can stimulate the immune system. As used herein immunostimulatory agents include, but are not limited to, vaccine adjuvants, such as Toll-like receptor agonists, T-cell checkpoint blockers, such as mAbs to PD-1 and CTL4 and T-cell checkpoint agonist, such as agonist mAbs to OX-40 and ICOS. As used herein "immunostimulatory agent" refers to any agent that can stimulate the immune system. As used herein immunostimulatory agents include, but are not limited to, vaccine adjuvants.

[0222] The term "Toll-like receptor" (or "TLR") as used herein refers to a member of the Toll-like receptor family of proteins or a fragment thereof that senses a microbial product and / or initiates an adaptive immune response. In one embodiment, a TLR activates a dendritic cell (DC). Toll-like receptors (TLRs) are a family of pattern recognition receptors that were initially identified as sensors of the innate immune system that recognize microbial pathogens. TLRs recognize distinct structures in microbes, often referred to as "PAMPs" (pathogen associated molecular patterns). Ligand binding to TLRs invokes a cascade of intra-cellular signaling pathways that induce the production of factors involved in inflammation and immunity. In humans, ten TLR have been identified. TLRs that are expressed on the surface of cells include TLR-1, -2, -4, -5, and -6, while TLR-3, -7 / 8, and -9 are expressed with the ER compartment. Human DC subsets can be identified on the basis of distinct TLR expression patterns. By way of example, the myeloid or "conventional" subset of DC (mDC) expresses TLRs 1-8 when stimulated, and a cascade of activation markers (e.g. CD80, CD86, MHC class I and II, CCR7), pro-inflammatory cytokines, and chemokines are produced. A result of this stimulation and resulting expression is antigen-specific CD4+ and CD8+ T cell priming. These DCs acquire an enhanced capacity to take up antigens and present them in an appropriate form to T cells. In contrast, the plasmacytoid subset of DC (pDC) expresses only TLR7 and TLR9 upon activation, with a resulting activation of NK cells as well as T-cells. As dying tumor cells may adversely affect DC function, it has been suggested that activating DC with TLR agonists may be beneficial for priming anti-tumor immunity in an immunotherapy approach to the treatment of cancer. It has also been suggested that successful treatment of breast cancer using radiation and chemotherapy requires TLR4 activation.

[0223] TLR agonists known in the art and finding use in the present invention include, but are not limited to, the following: Pam3Cys, a TLR1 / 2 agonist; CFA, a TLR2 agonist; MALP2, a TLR2 agonist; Pam2Cys, a TLR2 agonist; FSL-I, a TLR-2 agonist; Hib-OMPC, a TLR-2 agonist; polyinosinic:polycytidylic acid (Poly I:C), a TLR3 agonist; polyadenosine-polyuridylic acid (poly AU), a TLR3 agonist; Polyinosinic-Polycytidylic acid stabilized with poly-L-lysine and carboxymethylcellulose (Hiltonol), a TLR3 agonist; bacterial flagellin a TLR5 agonist; imiquimod, a TLR7 agonist; resiquimod, a TLR7 / 8 agonist; loxoribine, a TLR7 / 8 agonist; and unmethylated CpG dinucleotide (CpG-ODN), a TLR9 agonist.

[0224] Additional TLR agonists known in the art and finding use in the present invention further include, but are not limited to aminoalkyl glucosaminide phosphates (AGPs) which bind to the TLR4 receptor are known to be useful as vaccine adjuvants and immunostimulatory agents for stimulating cytokine production, activating macrophages, promoting innate immune response, and augmenting antibody production in immunized animals. An example of a naturally occurring TLR4 agonist is bacterial LPS. An example of a semisynthetic TLR4 agonist is monophosphoryl lipid A (MPL). AGPs and their immunomodulating effects via TLR4 are disclosed in patent publications such as WO 2006 / 016997, WO 2001 / 090129, and / or U.S. Patent No. 6,113,918 and have been reported in the literature. Additional AGP derivatives are disclosed in U.S. Patent No. 7,129,219, U.S. Patent No. 6,525,028 and U.S. Patent No 6,911,434. Certain AGPs act as agonists of TLR4, while others are recognized as TLR4 antagonist.

[0225] In one embodiment the immunostimulatory agent for use in combination with the compounds of the present invention is a TLR4 agonist. In one embodiment, the TLR4 agonist are referred to as CRX-601 and CRX-527. Their structures are set forth as follows:

[0226] Additionally, another preferred embodiment employs the TLR4 agonist CRX 547 having the structure shown.

[0227] Still other embodiments include AGPs such as CRX 602 or CRX 526 providing increased stability to AGPs having shorter secondary acyl or alkyl chains.

[0228] Thus, in one embodiment, there is provided (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof, or a pharmaceutically acceptable salt thereof, or a hydrate thereof in combination with at least one immunestimulatory agent for the treatment of a human in need thereof. In one embodiment, the immunostimulatory agent is a TLR4 agonist. In one embodiment, the immunostimulatory agent is an AGP. In yet another embodiment, the TLR4 agonist is selected from a compound having the formula CRX-601, CRX-527, CRX-547, CRX-602 or CRX-526. In one embodiment, the human has cancer.

[0229] In addition to the immunostimulatory agents described above, the compositions of the present invention may further comprise other therapeutic agents which, because of their adjuvant nature, can act to stimulate the immune system to respond to the cancer antigens present on the inactivated tumor cell(s). Such adjuvants include, but are not limited to, lipids, liposomes, inactivated bacteria which induce innate immunity (e.g., inactivated or attenuated Listeriamonocytogenes), compositions which mediate innate immune activation via, (NOD)-like receptors (NLRs), Retinoic acid inducible gene-based (RIG)-I-like receptors (RLRs), and / or C-type lectin receptors (CLRs). Examples of PAMPs include lipoproteins, lipopolypeptides, peptidoglycans, zymosan, lipopolysaccharide, neisserial porins, flagellin, profillin, galactoceramide, muramyl dipeptide. Peptidoglycans, lipoproteins, and lipoteichoic acids are cell wall components of Gram-positive. Lipopolysaccharides are expressed by most bacteria, with MPL being one example. Flagellin refers to the structural component of bacterial flagella that is secreted by pathogenic and commensal bacteria. rt.-Galactosylceramide (rt.-GalCer) is an activator of natural killer T (NKT) cells. Muramyl dipeptide is a bioactive peptidoglycan motif common to all bacteria.

[0230] Because of their adjuvant qualities, TLR agonists are preferably used in combinations with other vaccines, adjuvants and / or immune modulators, and may be combined in various combinations. Thus, in certain embodiments, (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide or a tautomer thereof, or a pharmaceutically acceptable salt thereof, or a hydrate thereof that bind to STING and induce STING-dependent TBKI activation and an inactivated tumor cell which expresses and secretes one or more cytokines which stimulate DC induction, recruitment and / or maturation, as described herein can be administered together with one or more TLR agonists for therapeutic purposes.

[0231] Indoleamine 2,3-dioxygenase 1 (ID01) is a key immunosuppressive enzyme that modulates the anti-tumor immune response by promoting regulatory T cell generation and blocking effector T cell activation, thereby facilitating tumor growth by allowing cancer cells to avoid immune surveillance. (Lemos H, et al., Cancer Res. 2016 Apr 15;76(8):2076-81), (Munn DH, et at., Trends Immunol. 2016 Mar;37(3): 193-207). Further active ingredients (anti-neoplastic agents) for use in combination or co-administered with the compounds of the invention are IDO inhibitors. Epacadostat, ((Z)-N-(3-bromo-4-fluorophenyl)-N'-hydroxy-4-[2-(sulfamoylamino)ethylamino]-1,2,5-oxadiazole-3-carboxamidine) is a highly potent and selective oral inhibitor of the IDOl enzyme that reverses tumor-associated immune suppression and restores effective anti-tumor immune responses. Epacadostat is disclosed in US patent No. 8,034,953.

[0232] Additional examples of other therapeutic agents (anti-neoplastic agent) for use in combination or co-administered with a compound of the invention are CD73 inhibitors and A2a and A2b adenosine antagonists.

[0233] In one embodiment, the compound of the invention may be employed with other therapeutic methods of treating infectious disease. In particular, antiviral and antibacterial agents are envisaged.

[0234] The compounds of the invention may be used in combination with at least one other therapeutic agent useful in the prevention or treatment of bacterial and viral infections. Examples of such agents include, without limitation: polymerase inhibitors such as those disclosed in WO 2004 / 037818-A1, as well as those disclosed in WO 2004 / 037818 and WO 2006 / 045613; JTK-003, JTK-019, NM-283, HCV-796, R-803, R1728, R1626, as well as those disclosed in WO 2006 / 018725, WO 2004 / 074270, WO 2003 / 095441, US2005 / 0176701, WO 2006 / 020082, WO 2005 / 080388, WO 2004 / 064925, WO 2004 / 065367, WO 2003 / 007945, WO 02 / 04425, WO 2005 / 014543, WO 2003 / 000254, EP 1065213, WO 01 / 47883, WO 2002 / 057287, WO 2002 / 057245 and similar agents; replication inhibitors such as acyclovir, famciclovir, ganciclovir, cidofovir, lamivudine and similar agents; protease inhibitors such as the HIV protease inhibitors saquinavir, ritonavir, indinavir, nelfinavir, amprenavir, fosamprenavir, brecanavir, atazanavir, tipranavir, palinavir, lasinavir, and the HCV protease inhibitors BILN2061, VX-950, SCH503034; and similar agents; nucleoside and nucleotide reverse transcriptase inhibitors such as zidovudine, didanosine, lamivudine, zalcitabine, abacavir, stavudine, adefovir, adefovir dipivoxil, fozivudine, todoxil, emtricitabine, alovudine, amdoxovir, elvucitabine, tenofovir disproxil fumarate, tenofovir alafenamide fumarate / hemifumarate, and similar agents; non-nucleoside reverse transcriptase inhibitors (including an agent having anti-oxidation activity such as immunocal, oltipraz etc.) such as nevirapine, delavirdine, efavirenz, loviride, immunocal, oltipraz, capravirine, TMC-278, TMC-125, etravirine, rilpivirine and similar agents; entry inhibitors such as enfuvirtide (T-20), T-1249, PRO-542, PRO-140, TNX-355, BMS-806, 5-Helix and similar agents; integrase inhibitors such as dolutegravir, elvitegravir, raltegravir L-870,180 and similar agents; budding inhibitors such as PA-344 and PA-457, and similar agents; chemokine receptor inhibitors such as vicriviroc (Sch-C), Sch-D, TAK779, maraviroc (UK-427,857), TAK449, as well as those disclosed in WO 02 / 74769, WO 2004 / 054974, WO 2004 / 055012, WO 2004 / 055010, WO 2004 / 055016, WO 2004 / 055011, and WO 2004 / 054581, and similar agents; pharmacokinetic enhancers such as cobicistat; neuraminidase inhibitors such as CS-8958, zanamivir, oseltamivir, peramivir and similar agents; ion channel blockers such as amantadine or rimantadine and similar agents; and interfering RNA and antisense oligonucleotides and such as ISIS-14803 and similar agents; antiviral agents of undetermined mechanism of action, for example those disclosed in WO 2005 / 105761, WO 2003 / 085375, WO 2006 / 122011, ribavirin, and similar agents.

[0235] The compounds of the invention may also be used in combination with other therapeutic agents which may be useful in the treatment of Kaposi's sarcoma-associated herpesvirus infections (KSHV and KSHV-related) include, without limitation chemotherapeutic agents such as bleomycin, vinblastine, vincristine, cyclophosphamide, prednisone, alitretinoin and liposomal anthracyclines such as doxorubicin, daunorubicin, immunotherapeutics such as Rituximab, Tocilizumab, Siltuximab and others such as Paclitaxel and Rapamycin.

[0236] In one embodiment of this invention, the at least one other therapeutic agent is an antimycobacterial agent or a bactericidal antibiotic. The compounds of the invention may also be used in combination with at least one other therapeutic agent which may be useful in the treatment of TB infection (Mycobacterium tuberculosis) and Tularemia (Francisella tularensis) include without limitation to first line oral agents isoniazid, Rifampicin, pyrazinamide, ethambutol, streptomycin, rifabutin; injectable agents including kanamycin, amikacin, capreomycin, streptomycin; fluoroquinolones including levofloxacin moxifloxacin ofloxacin; oral bacteriostatic agents para-aminosalicylic acid cycloserine terizidone thionamide protionamide; SQ-109 PNU-100480, Rifapentine Linezolid, PA-824 AZD5847, Gatifloxacin Moxifloxacin, Sirturo (bedaquiline) Delamanid (OPC-67683) and agents with undetermined mechanism of action in the treatment of drug-resistant TB, including clofazimine, linezolid, amoxicillin / clavulanate thioacetazone imipenem / cilastatin high dose isoniazid clarithromycin, ciprofloxacin. The compounds of the invention may also be used in combination with an antimycobacterial agent (such as isoniazid (INH), ehambutol (Myambutol ®< ), rifampin (Rifadin ®< ), and pyrazinamide (PZA)) a bactericidal antibiotic (such as rifabutin (Mycobutin ®< ) or rifapentine (Priftin ®< )), an aminoglycoside (Capreomycin ®< ), a fluorquinolone (levofloxacin, moxifloxicin, ofloxacin), thioamide (ehionamide), cyclosporine (Sandimmune ®< ), para-aminosalicyclic acid (Paser ®< ),cycloserine (Seromycin ®< ), kanamycin (Kantrex ®< ), streptomycin, viomycin, capreomycin (Capastat ®< )), bedaquiline fumarate (Sirturo ®< ), oxazolidinone (Sutezolid ®< ), PNU-100480, or delamanid (OPC-67683).

[0237] The compounds of the invention may also be used in combination with at least one other therapeutic agent which may be useful in the treatment of Chlamydia include, without limitations Azithromycin, Doxycycline, Erythromycin, Levofloxacin, Ofloxacin.

[0238] The compounds of this invention may also be used in combination with at least one other therapeutic agent which may be useful in the treatment of plasmodium infection include, without limitations to chloroquine, atovaquone-proguanil, artemether-lumefantrine, mefloquine, quinine, quinidine, doxocycline, cindamycin, artesunate, primaquine.

[0239] In the treatment of amyotrophic lateral sclerosis (ALS), a compound of the invention may be used in combination with a glutamate blocker (Riluzole (Rilutek ®< )), quinidine (Nuedexta ®< ), anticholinergics (amitriptyline ®< , Artane ®< , scopolamine patch (Transderm Scop ®< )), sympathomimetics (pseudoephedrine), mucolytics (guaifenesin), or analgesics (tramadol (Ultram ®< ); ketorolac (Toradol ®< ); morphine; fentanyl patch (Duragesic ®< )).

[0240] In the treatment of multiple sclerosis, a compound of the invention may be used in combination with corticosteroids (prednisone, methylprednisolone), Interferon Beta-1A (Avonex ®< , Extavia ®< , Rebif ®< , Betaseron ®< ), peginterferon beta-1A (Plegridy ®< ), Glatiramer acetate (Copaxone ®< ); glatiramer acetate (Glatopa ®< -generic equivalent of Copaxone); Dimethyl fumarate (Tecfidera ®< ); Fingolimod (Gilenya ®< )); teriflunomide (Aubagio ®< ); dalfampridine (Ampyra ®< ); daclizumab (Zinbryta); alemtuzumab (Lemtrada ®< ); natalizumab (Tysabri ®< ); or mitoxantrone hydrochloride (Novantrone ®< ).

[0241] The compounds of this invention may also be used as adjuvants to improve the immune response raised to any given antigen and / or reduce reactogenicity / toxicity in a patient, particularly a human, in need therof. As such, a compound of this invention may be used in combination with vaccine compositions to modify, especially to enhance, the immune response for example by increasing the level or duration of protection and / or allowing a reduction in the antigenic dose.

[0242] The compounds of the invention may be used in combination with one or more vaccines or immunogenic antigens useful in the prevention or treatment of viral infections. Such vaccines or immunogenic antigens include, without limitation to pathogen derived proteins or particles such as attenuated viruses, virus particles, and viral proteins typically used as immunogenic substances. Examples of viruses and viral antigens include, without limitations to Polioviruses, Cioronaviridae and Coronaviruses, Rhinovirus (all subtypes), Adenoviruses (all subtypes), Hepatitis A, Hepatitis B, Hepatitis C, Hepatitis D, Human papillomavirus (including all subtypes), Rabies viruses, Human T-cell lympotropic virus (all subtypes), Rubella virus, Mumps virus, Coxsackie virus A (all subtypes), Cosackie virus B (all subtypes), human enteroviruses, herpesviruses including cytomegalovirus, Epstein-Barr virus, human herepesviruses (all subtypes), herpes simplex virus, varicella zoster virus, human immunodeficiency virus (HIV) (all subtypes), Epstein-Barr virus, Reoviruses (all subtypes), Filoviruses including Marburg virus and Ebola virus (all stains), Arenaviruses including Lymphocytic choriomeningitis virus, Lassa virus, Junin virus, and Machupo virus, Arboviruses including West Nile virus, Dengue viruses (all serotypes), Zika virus, Colorado tick fever virus, Sindbis virus, Togaviraidae, Flaviviridae, Bunyaviridae, Reoviridae, Rhabdoviridae, Orthomyxoviridae, Poxviruses including orthopoxvirus (variola virus, monkypox virus, vaccinia virus, cowpox virus), yatapoxviruses (tanapox virus, Yaba monkey tumor virus), parapoxvirus, molluscipoxvirus, Yellow fever, Hantaviruses including Hantaan, Seoul, Dobrava, Sin Nombre, Puumala, and Dobrava-like Saaremaa, human para influenza viruses and influenza viruses (all types), H1N1 influenza and swine influenza viruses, respiratory syncytial virus (all subgroups), rotaviruses including human rotaviruses A-E, bovine rotavirus, rhesus monkey rotavirus, Polyomaviruses including simian virus 40, JC virus, BK virus, Coltiviruses, eyach virus, calciviruses, and Parvoviridae including dependovirus, parvovirus and erythrovirus.

[0243] Accordingly, this invention provides an immunogenic composition comprising an antigen or antigenic composition and a compound of the invention. There is further provided a vaccine composition comprising an antigen or antigenic composition and a compound of the invention.

[0244] The compounds of the invention may also be used in combination with at least one other therapeutic agent which may be useful in the prevention or treatment of viral infections for example immune therapies (e.g. interferon or other cytokines / chemokines, cytokine / chemokine receptor modulators, cytokine agonists or antagonists and similar agents); and therapeutic vaccines, anti-fibrotic agents, anti-inflammatory agents such as corticosteroids or NSAIDs (non-steroidal anti-inflammatory agents) and similar agents.

[0245] A compound that modulate STING, particularly a compound of the invention, may be administered in combination with other anti-inflammatory agents, including oral or topical corticosteroids, anti-TNF agents, 5-aminosalicyclic acid and mesalamine preparations, hydroxycloroquine, thiopurines, methotrexate, cyclophosphamide, cyclosporine, calcineurin inhibitors, mycophenolic acid, mTOR inhibitors, JAK inhibitors, Syk inhibitors, anti-inflammatory biologic agents, including anti-IL6 biologics, anti-IL1 agents, anti-IL17 biologics, anti-CD22, anti-integrin agents, anti-IFNa, anti-CD20 or CD4 biologics and other cytokine inhibitors or biologics to T-cell or B-cell receptors or interleukins.

[0246] For example, in the treatment of systemic lupus erythematosus and related lupus disorders, a compound that modulates STING, particularly a compound of the invention, may be administered in combination with at least one other therapeutic agent, including, a corticosteroid (such as prednisolone (Delatsone ®< , Orapred, Millipred, Omnipred, Econopred, Flo-Pred), an immunosuppressive agent (such as methotrexate (Rhuematrex ®< , Trexall ®< ), dexamethasone (Decadron ®< , Solurex ®< ), Mycophenolate mofetil (Cellcept ®< ), Tacrolimus ®< , Sirolimus ®< ), B-cell therapy (belimumab (Benlysta ®< ), B-cell inhibitor (Atacicept ®< , Apratuzumab ®< (anti-CD22), SBI-087 (anti-CD20), an anti-BAFF antibody (LY2127399, A623), Velcade ®< ), azathioprine (Azasan ®< , Imuran ®< ), triamcinolone (Clinacort ®< , Kenalog-10 ®< ), hydroxychloroquine (Plaquenil ®< ), thalidomide (Immunoprin ®< , Contergan ®< ), immunoglobulin therapy (HyQiva ®< , Flebogamma ®< , Gamunex ®< , Privigen ®< , Gammagard ®< ), anti-interferon-alpha therapy (Rontalizumab ®< , Sifalimumab ®< , AGS-009 ®< , IFN Kinoid), TLR7 and TLR9 blockers (IMO-3100), anti-cytokine therapies (anti-IL6 (CNTO-136), anti-interferon-gamma (AMG811), immunomodulatory therapy (Lupuzor ™< , Abatacept, Orencia ®< , AMG557, Laquinimod, Paquinimod, Leflunomide, anti-ICOS (Medi-570), anti-CD40 ligand antibody (CDP7657)), and / or a platelet aggregation inhibitor (aspirin).

[0247] In treatment of vasculitis and disease with inflammation of small or medium size blood vessels, a compound that modulates STING, particularly a compound of the invention, may be administered in combination with alkylating agents (cyclophosphamide, Cytoxan ®< ), anti-rheumatic anti-CD20 antibody (Rituxan ®< , Rituximab ®< ), and anti-TNFa inhibitors (Etanrcept ®< ).

[0248] In the treatment of psoriasis, a compound that modulates STING, particularly a compound of the invention, may be administered in combination with ixekizumab, tildrakizumab (MK-3222), or secukinumab (AIN457).

[0249] In one embodiment of this invention, the at least one other therapeutic agent is selected from an inhaled corticosteroid, a long acting beta agonist, a combination of an inhaled corticosteroid and a long acting beta agonist, a short acting beta agonist, a leukotriene modifier, an anti-IgE, a methylxanthine bronchodilator, a mast cell inhibitor, and a long-acting muscarinic antagonist. For example, in the treatment of asthma, a compound that inhibits STING, particularly a compound of the invention, may be administered in combination with an inhaled corticosteroid ((ICS) such as fluticasone propionate (Flovent ®< ), beclomethasone dipropionate (QVAR ®< ), budesonide (Pulmicort), trimcinolone acetonide (Azmacort ®< ), flunisolide (Aerobid ®< ), mometasone fuorate (Asmanex ®< Twisthaler ®< ), or Ciclesonide (Alvesco ®< )), a long acting beta agonist ((LABA) such as formoterol fumarate (Foradil ®< ), salmeterol xinafoate (Serevent ®< )), a combination of an ICS and LABA (such as fluticasone furoate and vilanterol (Breo Ellipta ®< ), formoterol / budesonide inhalation (Symbicort ®< ), beclomethasone dipropionate / formoterol (Inuvair ®< ), and fluticasone propionate / salmeterol (Advair ®< ), a short acting beta agonist ((SABA) such as albuterol sulfate (ProAir ®< , Proventil HFA ®< , Ventolin HFA ®< , AccuNeb ®< Inhalation Solution), levalbuterol tartrate (Xopenex ®< HFA), ipratropium bromide / albuterol (Combivent ®< Respimat ®< ), ipratropium bromide (Atrovent ®< HFA), a leukotriene modifier (such as montelukast sodium (Singulair ®< ), zafirlukast (Accolate ®< ),or zileuton (Zyflo ®< ), and anti-IgE (such as omalizumab (Xolair ®< )), a methylxanthine bronchodilator (such as theophylline (Accurbron ®< , Aerolate ®< , Aquaphyllin ®< , Asbron ®< , Bronkodyl ®< , Duraphyl ®< , Elixicon ®< , Elixomin ®< , Elixophyllin ®< , Labid ®< , Lanophyllin ®< , Quibron-T ®< , Slo-Bid ®< , Slo-Phyllin ®< , Somophyllin ®< , Sustaire ®< , Synophylate ®< , T-Phyll ®< , Theo-24 ®< , Theo-Dur ®< , Theobid ®< , Theochron ®< , Theoclear ®< , Theolair ®< , Theolixir ®< , Theophyl ®< , Theovent ®< , Uni-dur ®< , Uniphyl ®< ), a mast cell inhibitor (such as cromulyn sodium (Nasalcrom ®< ) and nedocromil sodium (Tilade ®< )), a long-acting muscarinic antagonist ((LAMA) such as mometasone furoate / formoterol fumarate dihydrate (Dulera ®< )).

[0250] Other agents that may be suitable for use in combination therapy in the treatment of asthma include a protein tyrosine kinase inhibitor (masitinib), CRTH2 / D-prostanoid receptor antagonist (AMG 853), indacaterol (Arcapta ®< Neohaler ®< ), an epinephrine inhalation aerosol (E004), fluticasone furoate / fluticasone proprionate, vinanterol inhalation / fluticasone furoate powder (Relovair ™< ), fluticasone propionate / eformoterol fumarate dehydrate (Flutiform ®< ), reslizumab, salbutamol dry-powder inhalation, tiotropium bromide (Spiriva ®< HandiHaler ®< ), formoterol / budesonide (Symbicort ®< SMART ®< ), fluticasone furoate (Veramyst ®< ), Vectura's VR506, lebrikizumab (RG3637), a combination phosphodiesterase (PDE)-3 and (PDE)-4 inhibitor (RPL554).

[0251] In one embodiment of this invention, the at least one other therapeutic agent is selected from a long acting beta agonist, a long-acting inhaled anticholinergic or muscarinic antagonist, a phosphodiesterase inhibitor, a combination an inhaled corticosteroid long acting beta agonist, a short acting beta agonist, and an inhaled corticosteroid. For example, in the treatment of COPD, a compound that modulates STING, particularly a compound of the invention, may be administered in combination with a LABA (such as salmeterol xinafoate (Serevent), umeclidinium / vilanterol (Anuro Ellipta ®< ), umeclidinium (Incruse Ellipta ®< ), aformoterol tartrate (Brovana ®< ), formoterol fumarate inhalation powder (Foradil ®< ), indacterol maleate (Arcapta ®< Neohaler ®< ), or fluticasone propionate / eformoterol fumarate dehydrate (Flutiform ®< )), a long-acting inhaled anticholinergic (or muscarinic antagonist, such as tiotropium bromide (Spiriva ®< ), and aclidinium bromide (Tudorza ®< Pressair ®< ), a phosphodiesterase (PDE-r) inhibitor (such as roflumilast, Daliresp ®< ), a combination ICS / LABA (such as fluticasone furoate and vilanterol (Breo Ellipta ®< ), fluticasone propionate / salmeterol (Advair ®< ), budesonide / formoterol (Symbicort ®< ), mometasone / formoterol (Dulera ®< ), ipratropium bromide / albuterol sulfate (Duoneb ®< , Atrovent ®< ), albuterol / ipratropium (Combivent Respimat ®< )), a SABA (such as ipratropium bromide (Atrovent ®< ), and albuterol sulfate(ProAir ®< ,Proventil ®< )), and an ICS (such as budesonide (Pulmicort ®< ) and fluticasone propionate (Flovent ®< ), beclometasone dipropionate (QVAR ®< ).

[0252] Other agents that may be suitable for use in combination therapy in the treatment of COPD include SCH527123 (a CXCR2 antagonist), glycoprronium bromide ((NVA237) Seebri ®< Breezhaler ®< ), glycopyrronium bromide and indacaterol maleate ((QVA149) Ultibro ®< Breezhaler ®< ), glycopyrrolate and formoterol fumarate (PT003), indacaterol maleate (QVA149), olodaterol (Striverdi ®< Respimat ®< ), tiotropium (Spiriva ®< ) / olodaterol (Striverdi ®< Respimat ®< ), and aclidinium / formoterol inhalation.

[0253] In one embodiment of this invention, the at least one other therapeutic agent is selected from an oral corticosteroid, anti-thymocyte globulin, thalidomide, chlorambucil, a calcium channel blocker, a topical emollient, an ACE inhibitor, a serotonin reuptake inhibitor, an endothelin-1 receptor inhibitor, an anti-fibrotic agent, a proton-pump inhibitor or imatinib, ARG201, and tocilizumab. For example, in the treatment of systemic scleroderma, a compound that modulates STING, particularly a compound of the invention, may be administered in combination with an oral corticosteroid (such as prednisolone (Delatsone ®< , Orapred, Millipred, Omnipred, Econopred, Flo-Pred), an immunosuppressive agent (such as methotrexate (Rhuematrex ®< , Trexall ®< ), cyclosporine (Sandimmune ®< ), anti-thymocyte globulin (Atgam ®< ), mycophenolate mofetil (CellCept ®< ), cyclophosphamide (Cytoxan ®< ), FK506 (tacrolimus), thalidomide (Thalomid ®< ), chlorambucil (Leukeran ®< ), azathioprine (Imuran ®< , Azasan ®< )), a calcium channel blocker (such as nifedipine (Procardia ®< , Adalat ®< ) or nicardipine (Cardene ®< ), a topical emollient (nitroglycerin ointment), an ACE inhibitor (such as lisinopril (Zestril ®< , Prinivil ®< ), diltaizem (Cardizem ®< , Cardizem SR ®< , Cardizem CD ®< , Cardia ®< , Dilacor ®< , Tiazac ®< )), a serotonin reuptake inhibitor (such as fluoxetine (Prozac ®< )), an endothelin-1 receptor inhibitor (such as bosentan (Tracleer ®< ) or epoprostenol (Flolan ®< , Veletri ®< , Prostacyclin ®< )) an anti-fibrotic agent (such as colchicines (Colcrys ®< ), para-aminobenzoic acid (PABA), dimethyl sulfoxide (DMSO), and D-penicillamine (Cuprimine ®< , Depen ®< ), interferon alpha and interferon gamma (INF-g)), a proton-pump Inhibitor (such as omeprazole (Prilosec ®< ), metoclopramide (Reglan ®< ), lansoprazole (Prevacid ®< ), esomeprazole (Nexium ®< ), pantoprazole (Protonix ®< ), rabeprazole (Aciphex ®< )) or imatinib (Gleevec ®< ) ARG201 (arGentis Pharmaceutical), belimumab (Benlysta ®< ), tocilizumab (Actema ®< ).

[0254] In the treatment of Sjögren's syndrome, a compound that modulates STING, particularly a compound of the invention, may be administered in combination with anti-rheumatic agents (hydroxychloroquine and Plaquenil ®< , Ridaura ®< , Kineret ®< ), cholinergic agonists (Salagen ®< , Evoxac ®< ), a JAK inhibitor (Xelijanz ®< , and anti-TNFa treatments (Remicade ®< , Humira ®< , Enbrel ®< , Cimzia ®< , Simponi ®< ).

[0255] In one embodiment of this invention, the at least one other therapeutic agent is a ciliary neurtotrophic growth factor or a gene transfer agent. For example, in the treatment of retinitis pigmentosa, a compound that modulates STING, particularly a compound of the invention, may be administered in combination with a ciliary neurtotrophic growth factor (NT-501-CNTF) or gene transfer agent, UshStat ®< .

[0256] In one embodiment of this invention, the at least one other therapeutic agent is selected from a trivalent (IIV3) inactivated influenza vaccine, a quadrivalent (IIV4) inactivated influenza vaccine, a trivalent recombinant influenza vaccine, a quadrivalent live attenuated influenza vaccine, an antiviral agent, or inactivated influenza vaccine. For example, in the treatment of influenza, a compound that modulates STING, particularly a compound of the invention, may be administered in combination with a trivalent (IIV3) inactivated influenza vaccine (such as Afluria ®< , Fluarix ®< , Flucelvax ®< , FluLaval ®< , Fluvirin ®< , Fluzone ®< ), a quadrivalent (IIV4) inactivated influenza vaccine (such as Fluarix ®< Quadrivalent, Flulaval ®< Quadrivalent, Fluzone ®< Quadrivalent), a trivalent recombinant influenza vaccine (such as FluBlok ®< ), a quadrivalent live attenuated influenza vaccine (such as FluMist ®< Quadrivalent), an antiviral agent (such as oseltamivir (Tamiflu ®< ), zanamivir (Relenza ®< ), rimantadine (Flumadine ®< ), or amantadine (Symmetrel ®< )), or Fluad ®< , Fludase, FluNhance ®< , Preflucel, or VaxiGrip ®<

[0257] In the treatment of a staphylococcus infection, a compound that modulates STING, particularly a compound of the invention, may be administered in combination with an antibiotic (such as a β-Lactam cephalosporin (Duricef ®< , Kefzol ®< , Ancef ®< , Biocef ®< , etc), nafcillin (Unipen ®< ), a sulfonamide (sulfamethoxazole and trimethoprim (Bacrim ®< , Septra ®< ,) sulfasalazine (Azulfidine ®< ), acetyl sulfisoxazole (Gantrisin ®< ), etc), or vancomycin (Vancocin ®< )).

[0258] In one embodiment of this invention, the at least one other therapeutic agent is selected from a topical immunomodulator or calcineurin inhibitor, a topical corticosteroid, an oral corticosteroid, an interferon gamma, an antihistamine, or an antibiotic. For example, in the treatment of atopic dermatitis, a compound that modulates STING , particularly a compound of the invention, may be administered in combination with a topical immunomodulator or calcineurin inhibitor (such as pimecrolimus (Elidel ®< ) or tacrolimus ointment (Protopic ®< )), a topical corticosteroid (such as hydrocortizone (Synacort ®< , Westcort ®< ), betamethasone (Diprolene ®< ), flurandrenolide (Cordan ®< ), fluticasone (Cutivate ®< ), triamcinolone (Kenalog ®< ), fluocinonide (Lidex ®< ), and clobetasol (Temovate ®< )), an oral corticosteroid (such as hydrocortisone (Cortef ®< ), methylprednisolone (Medrol ®< ), or prednisolone (Pediapred ®< , Prelone ®< ), an immunosuppressant (such as cyclosporine (Neoral ®< ) or interferon gamma (Alferon N ®< , Infergen ®< , Intron A, Roferon-A ®< )), an antihistamine (for itching such as Atarax ®< , Vistaril ®< , Benadryl ®< ), an antibiotic (such as penicillin derivatives flucloxacillin (Floxapen ®< ) or dicloxacillin (Dynapen ®< ), erythromycin (Eryc ®< , T-Stat ®< , Erythra-Derm ®< , etc.)), anon-steroidal immunosuppressive agent (such as azathioprine (Imuran ®< , Azasan ®< ), methotrexate (Rhuematrex ®< , Trexall ®< ), cyclosporin (Sandimmune ®< ), or mycophenolate mofetil (CellCept ®< )).

[0259] The compounds of the invention may also be formulated with vaccines as adjuvants to modulate their activity. Such compositions may contain antibody(ies) or antibody fragment(s) or an antigenic component including but not limited to protein, DNA, live or dead bacteria and / or viruses or virus-like particles, together with one or more components with adjuvant activity including but not limited to aluminum salts, oil and water emulsions, heat shock proteins, lipid A preparations and derivatives, glycolipids, other TLR agonists such as CpG DNA or similar agents, cytokines such as GM-CSF or IL-12 or similar agents.

[0260] In a further aspect of the invention, there is provided a vaccine adjuvant comprising a compound of the invention. There is further provided a vaccine composition comprising a compound of the invention, and an antigen or antigen composition.

[0261] A therapeutically "effective amount" is intended to mean that amount of a compound that, when administered to a patient in need of such treatment, is sufficient to effective treat or prevent, as defined herein. Thus, e.g., a therapeutically effective amount of a compound of the invention, is a quantity of an inventive agent that, when administered to a human in need thereof, is sufficient to modulate the activity of STING such that a disease condition which is mediated by that activity is reduced, alleviated or prevented. The amount of a given compound that will correspond to such an amount will vary depending upon factors such as the particular compound (e.g., the potency ( P IC 50 ), efficacy (EC 50 ), and the biological half-life of the particular compound), disease condition and its severity, the identity (e.g., age, size and weight) of the patient in need of treatment, but can nevertheless be routinely determined by one skilled in the art. Likewise, the duration of treatment and the time period of administration (time period between dosages and the timing of the dosages, e.g., before / with / after meals) of the compound will vary according to the identity of the mammal in need of treatment (e.g., weight), the particular compound and its properties (e.g., pharmacokinetic properties), disease or disorder and its severity and the specific composition and method being used, but can nevertheless be determined by one of skill in the art.

[0262] "Treating" or "treatment" is intended to mean at least the mitigation of a disease or disorder in a patient. The methods of treatment for mitigation of a disease or disorder include the use of the compounds in this invention in any conventionally acceptable manner, for example for retardation, therapy or cure of a STING-mediated disease or disorder, as described hereinabove. In one embodiment, "treat" "treating" or "treatment" in reference to cancer refers to alleviating the cancer, eliminating or reducing one or more symptoms of the cancer, slowing or eliminating the progression of the cancer, and delaying the reoccurrence of the condition in a previously afflicted or diagnosed patient or subject.

[0263] "Prevent", "preventing" or "prevention" refers to the prophylactic administration of a drug to diminish the likelihood of the onset of or to delay the onset of a disease or biological manifestation thereof. Prophylactic therapy is appropriate, for example, when a subject is considered at high risk for developing cancer, such as when a subject has a strong family history of cancer or when a subject has been exposed to a carcinogen.

[0264] The compounds of the invention may be administered by any suitable route of administration, including both systemic administration and topical administration. Systemic administration includes oral administration, parenteral administration, transdermal administration, rectal administration, and administration by inhalation. Parenteral administration refers to routes of administration other than enteral, transdermal, or by inhalation, and is typically by injection or infusion. Parenteral administration includes intravenous, intramuscular, and subcutaneous injection or infusion. Inhalation refers to administration into the patient's lungs whether inhaled through the mouth or through the nasal passages. Topical administration includes application to the skin.

[0265] In addition to the above described routes of administration suitable for treatment of oncology, the pharmaceutical compositions may be adapted for administration by intratumoral or peritumoral injection. The intratumoral or peritumoral injection of a compound of the present invention directly into or adjacent to a single solid tumor is expected to elicit an immune response that can attack and destroy cancer cells throughout the body, substantially reducing and in some cases permanently eliminating the tumor from the diseased subject. The activation of the immune system in this manner to kill tumors at a remote site is commonly known as the abscopal effect and has been demonstrated in animals with multiple therapeutic modalities, (van derJeught, etal., Oncotarget, 2015, 6(3), 1359-1381). A further advantage of local or intratumoral or peritumoral administration is the ability to achieve equivalent efficacy at much lower doses, thus minimizing or eliminating adverse events that may be observed at much higher systemic doses (Marabelle, A., et al., Clinical Cancer Research, 2014, 20(7), p1747-1756).

[0266] The compounds of the invention may be administered once or according to a dosing regimen wherein a number of doses are administered at varying intervals of time for a given period of time. For example, doses may be administered one, two, three, or four times per day. Doses may be administered until the desired therapeutic effect is achieved or indefinitely to maintain the desired therapeutic effect. Suitable dosing regimens for a compound of the invention depend on the pharmacokinetic properties of that compound, such as absorption, distribution, and half-life, which can be determined by the skilled artisan. In addition, suitable dosing regimens, including the duration such regimens are administered, for a compound of the invention depend on the disease or disorder being treated, the severity of the disease or disorder being treated, the age and physical condition of the patient being treated, the medical history of the patient to be treated, the nature of concurrent therapy, the desired therapeutic effect, and like factors within the knowledge and expertise of the skilled artisan. It will be further understood by such skilled artisans that suitable dosing regimens may require adjustment given an individual patient's response to the dosing regimen or over time as individual patient needs change. Total daily dosages range from 1 mg to 2000 mg, preferably, total daily dosages range from 1 mg to 250 mg.

[0267] For use in therapy, the compounds of the invention will be normally, but not necessarily, formulated into a pharmaceutical composition prior to administration to a patient. Accordingly, the invention also is directed to pharmaceutical compositions comprising a compound of the invention and at least one pharmaceutically acceptable excipient.

[0268] The pharmaceutical compositions of the invention may be prepared and packaged in bulk form wherein an effective amount of a compound of the invention can be extracted and then given to the patient such as with powders, syrups, and solutions for injection. Alternatively, the pharmaceutical compositions of the invention may be prepared and packaged in unit dosage form. For oral application, for example, one or more tablets or capsules may be administered. A dose of the pharmaceutical composition contains at least a therapeutically effective amount of a compound of this invention. When prepared in unit dosage form, the pharmaceutical compositions may contain from 1 mg to 1000 mg of a compound of this invention.

[0269] As provided herein, unit dosage forms (pharmaceutical compositions) containing from 1 mg to 1000 mg of a compound of the invention may be administered one, two, three, or four times per day, preferably one, two, or three times per day, and more preferably, one or two times per day, to effect treatment of a STING-mediated disease or disorder.

[0270] The pharmaceutical compositions of the invention typically contain one compound of the invention. However, in certain embodiments, the pharmaceutical compositions of the invention contain more than one compound of the invention. In addition, the pharmaceutical compositions of the invention may optionally further comprise one or more additional therapeutic agents, (e.g., pharmaceutically active compounds).

[0271] As used herein, "pharmaceutically acceptable excipient" refers to a pharmaceutically acceptable material, composition or vehicle involved in giving form or consistency to the pharmaceutical composition. Each excipient must be compatible with the other ingredients of the pharmaceutical composition when commingled such that interactions which would substantially reduce the efficacy of the compound of the invention when administered to a patient and interactions which would result in pharmaceutical compositions that are not pharmaceutically acceptable are avoided. In addition, each excipient must of course be of sufficiently high purity to render it pharmaceutically acceptable.

[0272] The compounds of the invention and the pharmaceutically acceptable excipient or excipients will typically be formulated into a dosage form adapted for administration to the patient by the desired route of administration. Conventional dosage forms include those adapted for (1) oral administration such as tablets, capsules, caplets, pills, troches, powders, syrups, elixirs, suspensions, solutions, emulsions, sachets, and cachets; (2) parenteral administration such as sterile solutions, suspensions, and powders for reconstitution; (3) transdermal administration such as transdermal patches; (4) rectal administration such as suppositories; (5) inhalation such as aerosols and solutions; and (6) topical administration such as creams, ointments, lotions, solutions, pastes, sprays, foams, and gels.

[0273] Suitable pharmaceutically acceptable excipients will vary depending upon the particular dosage form chosen. In addition, suitable pharmaceutically acceptable excipients may be chosen for a particular function that they may serve in the composition. For example, certain pharmaceutically acceptable excipients may be chosen for their ability to facilitate the production of uniform dosage forms. Certain pharmaceutically acceptable excipients may be chosen for their ability to facilitate the production of stable dosage forms. Certain pharmaceutically acceptable excipients may be chosen for their ability to facilitate the carrying or transporting the compound or compounds of the invention once administered to the patient from one organ, or portion of the body, to another organ, or portion of the body. Certain pharmaceutically acceptable excipients may be chosen for their ability to enhance patient compliance.

[0274] Suitable pharmaceutically acceptable excipients include the following types of excipients: diluents, fillers, binders, disintegrants, lubricants, glidants, granulating agents, coating agents, wetting agents, solvents, co-solvents, suspending agents, emulsifiers, sweeteners, flavoring agents, flavor masking agents, coloring agents, anti-caking agents, humectants, chelating agents, plasticizers, viscosity increasing agents, antioxidants, preservatives, stabilizers, surfactants, and buffering agents. The skilled artisan will appreciate that certain pharmaceutically acceptable excipients may serve more than one function and may serve alternative functions depending on how much of the excipient is present in the formulation and what other ingredients are present in the formulation.

[0275] Skilled artisans possess the knowledge and skill in the art to enable them to select suitable pharmaceutically acceptable excipients in appropriate amounts for use in the invention. In addition, there are a number of resources that are available to the skilled artisan which describe pharmaceutically acceptable excipients and may be useful in selecting suitable pharmaceutically acceptable excipients. Examples include Remington's Pharmaceutical Sciences (Mack Publishing Company), The Handbook of Pharmaceutical Additives (Gower Publishing Limited), and The Handbook of Pharmaceutical Excipients (the American Pharmaceutical Association and the Pharmaceutical Press).

[0276] The pharmaceutical compositions of the invention are prepared using techniques and methods known to those skilled in the art. Some of the methods commonly used in the art are described in Remington's Pharmaceutical Sciences (Mack Publishing Company).

[0277] In one aspect, the invention is directed to a solid oral dosage form such as a tablet or capsule comprising an effective amount of a compound of the invention and a diluent or filler. Suitable diluents and fillers include lactose, sucrose, dextrose, mannitol, sorbitol, starch (e.g. corn starch, potato starch, and pre-gelatinized starch), cellulose and its derivatives (e.g. microcrystalline cellulose), calcium sulfate, and dibasic calcium phosphate. The oral solid dosage form may further comprise a binder. Suitable binders include starch (e.g. corn starch, potato starch, and pre-gelatinized starch), gelatin, acacia, sodium alginate, alginic acid, tragacanth, guar gum, povidone, and cellulose and its derivatives (e.g. microcrystalline cellulose). The oral solid dosage form may further comprise a disintegrant. Suitable disintegrants include crospovidone, sodium starch glycolate, croscarmelose, alginic acid, and sodium carboxymethyl cellulose. The oral solid dosage form may further comprise a lubricant. Suitable lubricants include stearic acid, magnesium stearate, calcium stearate, and talc. For example, tablets may be prepared using conventional methods and are formulated as follows: Compound, 5mg; Microcrystalline cellulose, 100mg; Lactose, 100mg; Sodium starch glycollate, 30mg; Magnesium stearate, 2mg; Total wt. 237mg. Capsules may be prepared using conventional methods and are formulated as follows: Compound, 15mg; dried starch, 178mg; Magnesium stearate, 2mg; Total wt. 195mg.

[0278] It will be understood that the compounds of this invention may also be formulated with vaccines as adjuvants to modulate their activity. Such compositions may contain antibody (antibodies) or antibody fragment(s) or an antigenic component including but not limited to protein, DNA, live or dead bacteria and / or whole, inactivated or split viruses or virus-like particles, recombinant proteins or antigenic fragments thereof, optionally together with one or more other components with adjuvant activity including but not limited to aluminum salts, oil and water emulsions, heat shock proteins, saponins, lipid A preparations and derivatives, glycolipids, liposomes, TLR agonists such as CpG DNA or similar agents, cytokines such as GM-CSF or IL-12, or similar agents.

[0279] Certain compounds of the invention may be potent immunomodulators and accordingly, care should be exercised in their handling.Examples

[0280] The following examples illustrate the invention. These examples are not intended to limit the scope of the present invention, but rather to provide guidance to the skilled artisan to prepare and use the compounds, compositions, and methods of the present invention. While particular embodiments of the present invention are described, the skilled artisan will appreciate that various changes and modifications can be made without departing from the spirit and scope of the invention.

[0281] It will be understood that certain compounds of the invention may be potent immunomodulators and accordingly, care should be exercised in their handling.

[0282] The reactions described herein are applicable for producing compounds of the invention having a variety of different substituent groups (e.g., R 1< , R 2< , etc.), as defined herein. The skilled artisan will appreciate that if a particular substituent is not compatible with the synthetic methods described herein, the substituent may be protected with a suitable protecting group that is stable to the reaction conditions. Suitable protecting groups and the methods for protecting and de-protecting different substituents using such suitable protecting groups are well known to those skilled in the art; examples of which may be found in T. W. Greene 'Protective Groups in Organic Synthesis' (4th edition, J. Wiley and Sons, 2006). Unless otherwise noted, all starting materials were obtained from commercial suppliers and used without further purification.

[0283] Certain intermediate compounds described herein form a yet further aspect of the invention.General Synthetic Methods

[0284] The compounds of this invention may be prepared using synthetic procedures illustrated in the reaction schemes below, which can be readily adapted to prepare other compounds of the invention by drawing on the knowledge of a skilled organic chemist. The syntheses provided in these schemes are applicable for producing compounds of the invention having a variety of different R groups employing appropriate precursors, which are suitably protected if needed, to achieve compatibility with the reactions outlined herein. Subsequent deprotection, where needed, affords compounds of the nature generally disclosed. While the schemes are shown with compounds only of Formula (I-N), (I-P) or (I), they are illustrative of processes that may be used to make the compounds of the invention. Intermediates (compounds used in the preparation of the compounds of the invention) may also be present as salts.

[0285] Method 1: An appropriate nitro-halo benzamide (1A) may be treated with an amine (allylamine used as an example) under base or metal-mediated coupling conditions to afford the aniline (1B). Subsequent reduction of the nitro group via appropriate conditions will provide dianiline (1C). Reaction with cyanogen bromide provides the aminobenzimidazole (1D). Peptide coupling between the aminobenzimiazole and pyrazole acid (1E) generates the amidobenzimidazole monomer (1F). Cross metathesis reaction between two molecules of (1F) affords the unsaturated dimer (1G), which can be hydrogenated to afford saturated dimer 1H.

[0286] Method 2: Two molecules of an appropriate nitro-halo benzamide (2A) may be treated with a diamine under base or metal-mediated coupling conditions to afford the biscoupled aniline (2B). Subsequent bis-reduction of the nitro groups via appropriate conditions can provide the dianiline (2C). Reaction with cyanogen bromide provides the aminobenzimidazole dimer (2D). Peptide coupling between bis amine (2D) and two molecules of pyrazole acid (2E) generates the amidobenzimidazole dimer (2F).

[0287] Method 3: Fluoronitrobenzamide (3A) is treated with a monoprotected diamine under base or metal-mediated coupling conditions to afford the aniline (3B). Subsequent reduction of the nitro group via appropriate conditions can provide the dianiline (3C). Reaction with cyanogen bromide provides the aminobenzimidazole (3D). Peptide coupling between amine (3D) and pyrazole acid (3E) generates the amidobenzimidazole (31), which can be deprotected under appropriate conditions for the amine protecting group to afford amine (3G). Amine (3G) can be coupled with an appropriate halonitrobenzamide (3H) to provide (31); reduction of the nitro group can provides dianiline (3J). Treatment of (3J) with cyanogen bromide generates aminobenzimidazole (3K), which can be treated with pyrazole acid (3E) under amide coupling conditions to afford the unsymmetrical dimer (3L).

[0288] Method 4: An appropriate nitro-halo benzamide (4A) is treated with a monoprotected diamine under base or metal-mediated coupling conditions to afford the allylaniline (4B). Subsequent reduction of the nitro group via appropriate conditions will provide the dianiline (4C). Reaction with cyanogen bromide provides the aminobenzimidazole (4D). Peptide coupling between amine (4D) and pyrazole acid (4E) generates the amidobenzimidazole (4F), which can be deprotected under appropriate conditions for the amine protecting group to afford amine (4G). Amine (4G) can be coupled with fluoronitrobenzamide (4H) to provide (41), and then reduction of the nitro group will provide dianiline (4J). Treatment of (4J) with cyanogen bromide generates aminobenzimidazole (4K), which can be treated with pyrazole acid (4E) under amide coupling conditions to afford the unsymmetrical dimer (4L).

[0289] Method 5 Two molecules of an appropriate phenol (5A) are reacted with a bis-electrophile such as dibromopropane to provide ether linked dimer (5B). Dimer (5B) is then reacted with a suitable diamine to afford macrocycle (5C). Reduction of the nitro groups, followed by treatment with cyanogen bromide affords bisaminobenzimidazole (5D). Amide coupling with an appropriate acid (5E) affords bisamidobenzimidazole macrocycle (5F).

[0290] Method 6: Bispyrazole acid 6A (Method 8) is reacted with Aminobenzimidazole dimer (6B) under amide coupling conditions to afford amidobenzimidazole macrocycle (6C), wherein each R may be the same or different.

[0291] Method 7: Bispyrazole acid 7A (Method 8) is reacted with two molecules of aminobenzimidazole (7B) under amide coupling conditions to afford pyrazole-linked dimer (7C). Ring closing metathesis reaction will afford the unsaturated macrocycle (7D), which can be hydrogenated to provide the saturated amidobenzimidazole macrocycle (7E).

[0292] Method 8: A substituted (pent-4-yn-1-yl)-1H-pyrazole-carboxylate (8C) may be formed by N-alkylation of a substituted 1H-pyrazole-carboxylate (8A) with (5-chloropent-1-yn-1-yl)trimethylsilane followed by de-silylation. A 4-iodo-1H-pyrazole-carboxylate (8F) may be formed by esterification of the corresponding 1H-pyrazole-carboxylic acid (8D), followed by iodination using 1-iodopyrrolidine-2,5-dione. Palladium catalyzed coupling of the substituted (pent-4-yn-1-yl)-1H-pyrazole-carboxylate (8C) with the 4-iodo-1H-pyrazole-carboxylate (8F) forms an alkynyl-linked bispyrazole (8G). Hydrogenation of the alkynyl-linked bispyrazole, followed by hydrolysis provided the bispyrazole acid used in Methods 6 and 7 (6A / 7A), above.

[0293] Method 9: An appropriate nitro-halo benzamide (9A) may be treated with a monoprotected diamine (such as 9B) under base or metal-mediated coupling conditions to afford the coupled aniline (9C). Subsequent deprotection of the primary amine will provide amine 9D. A second nitro-halo benzamide (9E) may be reacted with amine 9D under base or metal-mediated coupling conditions to afford a bis-nitro dimeric benzamide (9F). Double reduction of the nitro groups via appropriate conditions can provide the dianiline (9G). Reaction with cyanogen bromide provides the aminobenzimidazole dimer (9H). Peptide coupling between bis amine 9H and two molecules of pyrazole acid (91) generates the amidobenzimidazole dimer (9J).Method 9

[0294]

[0295] Method 10: A tetraaniline (10A, prepared by method 9 or another general method) may be treated with isothiocyanate (10B) until completion of the reaction. Upon completion of step 1, EDC (or other suitable coupling reagent) and triethylamine (or other suitable base) are added and the reaction stirred until completion to afford amidobenzimidazole dimer (10C).

[0296] Method 11: All variables are as defined in Formula (I-N), (I-P) or Formula (I). A suitably substituted halonitrobenzamide (11A) is reacted with a monoprotected diamine such as 11B to provide nitroaniline 11C. Deprotection of the amine protecting group affords amine 11D, which can be reacted with a halo-nitrophenyl compound 11E to afford bis-nitro 11F. Reduction of both nitro groups will provide a bis-aniline 11G which is treated with cyanogen bromide to afford bisaminobenzimidazole 11H. Amide coupling with a pyrazole acid such as 11I will afford a substituted amidobenzimidazole dimer 11J. When suitable functional groups are present on 11J, further functionalization of these groups will be possible to afford additional compounds such as 11K.

[0297] Method 12: In method 12, R C2< =R 14< , R 17< =R 15< and R 16< =R C1< , all other variables are as defined in Formula (I-N), Formula (I-P) or Formula (I). A tetraaniline (12A, prepared by method 11, 16 or another general method) may be treated with an isothiocyanate such as 12B until completion of the reaction. Upon completion of step 1, EDC (or other suitable coupling reagent) and triethylamine (or other suitable base) are added and the reaction stirred until completion to afford amidobenzimidazole dimer (12C).

[0298] Method 13: In method 13, all variables are as defined in Formula (I-N), Formula (I-P) or Formula (I). Macrocyclic compounds with substitutions can be prepared via this method. A substituted halonitrophenyl compound (13A) is treated with a suitable diamine (13B) containing a linker group (B) between the two amine groups to afford amine 13C. Reduction of the nitro group followed by treatment with cyanogen bromide can afford aminobenzimidazole 13D. Amide coupling between 13D and a mono-carboxylic acid bispyrazole (such as 13E) containing a linker group (C) between the two pyrazoles will afford an amidobenzimidazole (13F). Deprotection of the amine group enables addition to a second substituted halo-nitro-phenyl (13G) to provide nitro-ester 13H. Reduction of the nitro group of 13H followed by treatment with cyanogen bromide will provide aminobenzimidazole 131. Hydrolysis of the pyrazole ester then enables a macrocyclic amide formation to provide the macrocyclic amidobenzimidazole 13J. When suitable functional groups are present on 13J, further functionalization of these groups will be possible to afford additional compounds such as 13K.

[0299] Method 14: -O-M 1< is defined as optionally substituted (C 1 -C 6 alkyl)oxy as defined for R A1< when q is 0 in Formula (I) Formula (I-N), or Formula (I-P). All other variables are as defined in Formula (I-N), Formula (I-P) or Formula (I). A dimeric amidobenzimidazole containing a phenol, such as 14A, prepared via one of the general synthetic methods described here, can be alkylated on the phenol through the use of a suitable alkylating agent such as an alkyl bromide and base such as potassium carbonate. When suitable functional groups are present on 14B, further functionalization of these groups will be possible to afford additional compounds.

[0300] Method 15: All variables are as defined in Formula (I-N), Formula (I-P) or Formula (I). A suitably substituted halo-nitrophenyl compound (15A) is reacted with a diamine containing a linker group (B) such as 15B to provide bis-nitro dimer 15C. Reduction of both nitro groups will provide a tetraaniline 15D which can be converted to an amidobenzimidazole dimer (15E) via one of two methods: 1) Treatment with cyanogen bromide to afford a bisaminobenzimidazole followed by amide coupling with a pyrazole acid such as 15F; or 2) Treatment with isothiocyanate (15G) until completion of the reaction, then addition of EDC (or other suitable coupling reagent) and triethylamine (or other suitable base) and the reaction is stirred until completion. When suitable functional groups are present on 15E, further functionalization of these groups will be possible to afford additional compounds.

[0301] Method 16: All variables are as defined in Formula (I-N), Formula (I-P) or Formula (I). A suitably substituted halo-nitrophenyl compound (16A) is reacted with a monoprotected diamine containing a linker group (B) such as 16B to provide nitro-aniline 16C. Deprotection of the amine protecting group affords amine 16D, which can be reacted with a halo-nitrophenyl compound 16E to afford bis-nitro dimer 16F. Reduction of both nitro groups will provide a tetraaniline 16G which can be converted to an amidobenzimidazole dimer (16H) via one of two methods: 1) Treatment with cyanogen bromide to afford a bisaminobenzimidazole followed by amide coupling with a pyrazole acid such as 161; or 2) Treatment with isothiocyanate (16J) until completion of the reaction, then addition of EDC (or other suitable coupling reagent) and triethylamine (or other suitable base) and the reaction is stirred until completion. When suitable functional groups are present on 16H, further functionalization of these groups will be possible to afford additional compounds.

[0302] Method 17: M 2< is C 1 -C 6 alkyl or COOM 2< can be any ester that is inactive to hydrogenolysis of benzyl ester. All other variables are as defined in Formula (I-N), Formula (I-P) or Formula (I). A substituted pyrazole ester such as (pent-4-yn-1-yl)-1H-pyrazole-carboxylate (17D) may be formed by esterification of a substituted 1H-pyrazole-carboxylate (17A) to afford ester 17B followed by N-alkylation under mitsunobu conditions. A 4-iodo-pyrazole ester (17G) may be formed by esterification of the corresponding pyrazole-carboxylic acid (17E), followed by iodination using 1-iodopyrrolidine-2,5-dione (NIS). Palladium-catalyzed coupling of an alkylated pyrazole such as 17D with the 4-iodo-pyrazole-ester (17G) forms a linked bispyrazole (17H). Reduction and hydrogenolysis of the linked bispyrazole will provide a bispyrazole monoacid (171).

[0303] Method 18: all variables are as defined in Formula (A). (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-hydroxy-propoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide (18A) may be treated with methanesulfonyl chloride and triethylamine. Upon completion of step 1, the resulting mesylate (18B) is treated with an amine (NHR A< R B< ) and K 2 CO 3 and the reaction heated at 50-80 °C until completion to afford the desired compounds (18C).

[0304] Method 19: All variables are as defined in Formula (I-N), Formula (I-P) or Formula (I). A suitably substituted bis-aminobenzimidazole (19B) containing a linker group (B), prepared via one of the methods described here, is reacted with a bispyrazole (19A) incorporating a linker group (C) and amide coupling reagents to afford a macrocylic bisamidobenzimidazole.

[0305] Method 20: All variables are as defined in Formula (I-N), Formula (I-P) or Formula (I). A suitably substituted halo-nitrophenyl compound (20A) is reacted with a monoprotected diamine containing a linker group (B) such as 20B to provide nitro-aniline 20C. Reduction of the nitro group under appropriate conditions will afford dianiline 20D, which can be converted to an amidobenzimidazole 20F via one of two methods: 1) treatment with cyanogen bromide followed by amide coupling with a pyrazole acid such as 20E; or 2) treatment with isothiocyanate (20L) until completion of the reaction, then addition of EDC (or other suitable coupling reagent) and triethylamine (or other suitable base) and the reaction is stirred until completion. Deprotection of the amine protecting group affords amine 20G, which can be reacted with a halo-nitrophenyl compound 20H to afford dimeric nitro aniline 201. Reduction of the nitro group will provide bis-aniline 20J which can be converted to an amidobenzimidazole dimer (20K) via one of two methods: 1) Treatment with cyanogen bromide to afford a bisaminobenzimidazole followed by amide coupling with a pyrazole acid such as 20M; or 2) Treatment with isothiocyanate (20N) until completion of the reaction, then addition of EDC (or other suitable coupling reagent) and triethylamine (or other suitable base) and the reaction is stirred until completion. When suitable functional groups are present on 20K, further functionalization of these groups will be possible to afford additional compounds.

[0306] Method 21: All variables are as defined in Formula (I-N), Formula (I-P) or Formula (I). Two molecules of an appropriate functionalized nitro-halo-phenyl (21A) are dimerized to provide bis-nitro dimer 21B containing a linker group (A). 21B is then reacted with an amine or diamine to afford dianiline 21C. Reduction of the nitro groups, provides tetraaniline 21C which can be converted to an amidobenzimidazole dimer (21G) via one of two methods: 1) Treatment with cyanogen bromide to afford a bisaminobenzimidazole followed by amide coupling with a pyrazole acid such as 21E; or 2) Treatment with isothiocyanate (21F) until completion of the reaction, then addition of EDC (or other suitable coupling reagent) and triethylamine (or other suitable base) and the reaction is stirred until completion. When suitable functional groups are present on 21G, further functionalization of these groups will be possible to afford additional compounds. A general example of a dimerization would be the reaction of a suitable nitro-phenol (21H) with a bis-halide and a base to afford the bisphenol dimer 21J.

[0307] Method 22: All variables are as defined in Formula (I-N), Formula (I-P) or Formula (I). A suitably substituted aminobenzimidazole (22A), prepared via one of the methods described here, is reacted with a bispyrazole (22B) incorporating a linker group (C) and amide coupling reagents to afford a dimeric bisamidobenzimidazole. When suitable functional groups are present on 22C, further functionalization of these groups will be possible to afford additional compounds.

[0308] Method 23: All variables are as defined in Formula (I-N), Formula (I-P) or Formula (I). A suitably substituted aminobenzimidazole (23A), prepared via one of the methods described here, is reacted with a mono-carboxylic acid bispyrazole (23B) incorporating a linker group (C), and amide coupling reagents to afford an amidobenzimidazole ester such as 23C. Hydrolysis of the pyrazole ester will provide acid 23D, which can be coupled with a second aminobenzimidazole (23E) to provide a dimeric bisamidobenzimidazole (23F). When suitable functional groups are present on 23F, further functionalization of these groups will be possible to afford additional compounds.

[0309] Method 24: M 2< is C 1 -C 6 alkyl or COOM 2< can be any ester that is inactive to hydrogenolysis of benzyl ester. All other variables are as defined in Formula (I-N), Formula (I-P) or Formula (I). A substituted pyrazole ester such as (pent-4-yn-1-yl)-1H-pyrazole-carboxylate (24D) may be formed by esterification of a substituted 1H-pyrazole-carboxylate (24A) to afford ester 24B followed by N-alkylation under suitable conditions such as an alkyl halide and base. In the case of using with (5-chloropent-1-yn-1-yl)trimethylsilane, a subsequent desilation will afford pyrazole ester 24D. A 4-iodo-pyrazole ester (24G) may be formed by esterification of the corresponding pyrazole-carboxylic acid (24E), followed by iodination using 1-iodopyrrolidine-2,5-dione (NIS). Palladium-catalyzed coupling of an alkylated pyrazole such as 24D with the 4-iodo-pyrazole-ester (24G) forms a linked bispyrazole (24H). Reduction and hydrogenolysis of the linked bispyrazole will provide a bispyrazole monoacid (241), which can be further hydrolyzed to afford a bispyrazole di acid 24J.

[0310] It will be appreciated that in any of the routes described above, the precise order of the synthetic steps by which the various groups and moieties are introduced into the molecule may be varied. It will be within the skill of the practitioner in the art to ensure that groups or moieties introduced at one stage of the process will not be affected by subsequent transformations and reactions, and to select the order of synthetic steps accordingly.

[0311] Names for the intermediate and final compounds described herein were generated using the software naming programs ChemDraw Pro 12.0.2.1076 Plug-In inside of Perkin Elmer E-Notebook or MarvinSketch 5.11.4_b82 (Chemaxon).

[0312] It will be appreciated by those skilled in the art that in certain instances these programs may name a structurally depicted compound as a tautomer or isomer of that compound. It is to be understood that any reference to a named compound or a structurally depicted compound is intended to encompass all tautomers or isomers of such compounds and any mixtures of tautomers and / or isomers thereof.

[0313] The definitions for LCMS analysis conditions listed below and apply to all compounds. LCMS Method LCMS Method A Wavelength214 nm and 254 nmInstrumentAgilent 1200-6110ColumnHalo C18 4.6 × 50 umFlow Rate1.8 mL / minGradient MethodTime (min)ACN (0.05% FA)H 2 O (0.05% FA)0595195529552.5595 LCMS Method LCMS Method B Wavelength214 nm and 254 nmInstrumentShimadzu 2020ColumnHalo C18 4.6 × 50 umFlow Rate1.5 mL / minGradient Method Time (min)ACN (0.05% FA)H 2 O (0.05% FA)0595195549554.55955595 LCMS Method: LCMS Method C Instrumentation

[0314] LC:Shimadzu 10Avp (controller, pumps, and UV detector)UV:Shimadzu 10AVp (214nm)ELS:Sedere Sedex 75C (45C)MS:PE Sciex Single Quadrupole 150EXPolarity (positive); Mode (profile); Scan Time (0.33s); Step (0.2 m / z) Capillary V (5500); Cone V (25-45)or Waters ZQ Single QuadrupolePolarity (positive); Mode (continuum); Scan Time (0.25s)Capillary V (3500); Cone V (25-35)Autosampler:CTC Leap; 3uL loop; default injection volume = 2uL (default)Column:Thermo Hypersil Gold (C18, 20x2.1 mm, 1.9 u particle diam.)Heater:Phenomenex 50-55°CSolvent A:H 2 O, 0.02% TFASolvent B:MeCN, 0.02% TFAGradient:Time (min)Flow (mL / min)Sol. B0.021.44.01.9095.01.914.02.00Stop LCMS Method: LCMS Method D Instrumentation

[0315] LC:Waters Acquity Binary Solvent Manager, Column Manager 55CAutosampler:CTC Leap PAL AutosamplerUV:Waters Acquity PDA (210-360nm)ELS:Waters Acquity ELSD (50C) or Sedere Sedex 75C (45C)MS:Waters Acquity SQDPolarity (positive or negative); Mode (continuum); Scan Time (0.15s) Capillary V (3500); Cone V (25-35);Column:Thermo Hypersil Gold (C18, 20x2.1 mm, 1.9 u particle diam.)Solvent A:H 2 O, 0.02% TFASolvent B:MeCN, 0.02% TFAGradient:Time (min)Flow (mL / min)Sol. B0.021.62.01.9095.01.91stop4.0 LCMS Method: LCMS Method E Instrumentation

[0316] LC:Waters Acquity I-Class Binary Solvent Manager, Column Manager 55CAutosampler:CTC Leap PAL 3 AutosamplerUV:Waters Acquity PDA (210-360nm)ELS:Waters Acquity ELSD (50C) or Sedere Sedex 85C (45C)MS:Waters Acquity QDa Mass DetectorPolarity (positive or negative); Mode (continuum); Scan Time (10Hz) Capillary kV (0.8); Cone V (12);Column:Thermo Hypersil Gold (C18, 20x2.1 mm, 1.9 u particle diam.)Solvent A:H 2 O, 0.02% TFASolvent B:MeCN, 0.02% TFAGradient:Time (min)Flow (mL / min)Sol. B%0.021.60.51.9090 to 951.91stop0.5 LCMS Method: LCMS Method F Instrumentation

[0317] LC:Waters Acquity Binary Solvent Manager, Column Manager 55CAutosampler:CTC Leap PAL AutosamplerUV:Waters Acquity PDA (210-360nm)ELS:Waters Acquity ELSD (50C) or Sedere Sedex 75C (45C)MS:Waters Acquity SQDPolarity (positive or negative); Mode (continuum); Scan Time (0.15s) Capillary V (3500); Cone V (25-35);Column:Waters BEH (C18, 30x2.1 mm, 1.7 u particle diam.)Solvent A:H 2 O, 0.02% TFASolvent B:MeCN, 0.02% TFAGradient:Time (min)Flow (mL / min)Sol. B0.021.51.04.9085.04.911.05.00stop1.0 LCMS Method: LCMS Method G

[0318] The UPLC analysis was conducted on an Acquity UPLC CSH C18 column (50mm × 2.1mm i.d. 1.7µm packing diameter) at 40 degrees centigrade.

[0319] The solvents employed were: A = 0.1% v / v solution of Formic Acid in Water. B = 0.1% v / v solution of Formic Acid in Acetonitrile.

[0320] The gradient employed was: Time (min)Flow Rate (mL / min)%A%B019731.515951.915952.01973

[0321] The UV detection was a summed signal from wavelength of 210nm to 350nm. Injection volume :0.5ul MS Conditions

[0322] MS :Waters ZQIonisation mode :Alternate-scan Positive and Negative Electrospray Scan LCMS Method: LCMS Method H

[0323] The UPLC analysis was conducted on an Acquity UPLC CSH C18 column (50mm × 2.1mm i.d. 1.7µm packing diameter) at 40 degrees centigrade.

[0324] The solvents employed were: A = 10 mM Ammonium Bicarbonate in water adjusted to pH 10 with ammonia solution. B = Acetonitrile.

[0325] The gradient employed was: Time (min)Flow Rate (mL / min)%A%B019730.0519731.515951.915952.01973

[0326] The UV detection was a summed signal from wavelength of 210nm to 350nm. Injection volume :0.3ul MS Conditions

[0327] MS :Waters ZQIonisation mode :Alternate-scan Positive and Negative Electrospray LCMS Method: LCMS Method I

[0328] The UPLC analysis was conducted on an Acquity UPLC CSH C18 column (50mm × 2.1mm i.d. 1.7µm packing diameter) at 40 degrees centigrade.

[0329] The solvents employed were: A = 10 mM Ammonium Bicarbonate in water adjusted to pH 10 with 25% ammonium hydroxide solution. B = Acetonitrile

[0330] The gradient employed was: Time (min)Flow Rate (mL / min)%A%B019730.0519731.515951.915952.01973

[0331] The UV detection was a summed signal from wavelength of 210nm to 350nm. Injection volume:0.5 uL MS Conditions

[0332] MS :Waters Acquity SQD or QDa mass detectorIonisation mode :Alternate-scan Positive and Negative LCMS Method: LCMS Method J

[0333] The UPLC analysis was conducted on an Acquity UPLC CSH C18 column (50mm × 2.1mm i.d. 1.7µm packing diameter) at 40 degrees centigrade.

[0334] The solvents employed were: A = 0.1% v / v solution of Formic Acid in Water. B = 0.1% v / v solution of Formic Acid in Acetonitrile.

[0335] The gradient employed was: Time (min)Flow Rate (mL / min)%A%B019731.515951.915952.01973

[0336] The UV detection was a summed signal from wavelength of 210nm to 350nm. Injection volume:0.5 uL MS Conditions

[0337] MS :Waters Acquity SQD or QDa mass detectorIonisation mode :Alternate-scan Positive and Negative LCMS Method: LCMS Method K

[0338] The UPLC analysis was conducted on an Acquity UPLC CSH C18 column (50mm × 2.1mm i.d. 1.7µm packing diameter) at 40 degrees centigrade.

[0339] The solvents employed were: A = 0.1% v / v solution of TFA in Water. B = 0.1% v / v solution of TFA in Acetonitrile.

[0340] The gradient employed was: Time (min)Flow Rate (mL / min)%A%B019731.515951.915952.01973

[0341] The UV detection was a summed signal from wavelength of 210nm to 350nm. Injection volume:0.5 uL MS Conditions

[0342] MS:Waters Acquity SQD or QDa mass detectorIonisation mode:Alternate-scan Positive and Negative Electrospray LCMS Method: LCMS Method L Instrumentation

[0343] LC:Waters Acquity I-Class Binary Solvent Manager, I-Class Column Manager 55CAutosampler:CTC PAL 3 AutosamplerUV:Waters Acquity PDA (210-360nm)ELS:Sedere Sedex 85C (45C)MS:Waters Acquity QDa Mass DetectorPolarity (positive or negative); Mode (continuum); Scan Time (10Hz)Capillary kV (0.8); Cone V (12);Column:Thermo Hypersil Gold (C18, 20x2.1 mm, 1.9 u particle diam.)Solvent A:H 2 O, 0.02% TFASolvent B:MeCN, 0.02% TFAGradient:Time (min)Flow (mL / min)Sol. B %0.021.60.51.90951.910.52.00stop LCMS Method: LCMS Method M

[0344] The LCMS analysis was conducted on a Waters Sunfire C18 column (50mm × 3.0mm i.d. 5µm packing diameter) at Ambient temperature on an Agilent 1200 HPLC with a Model 6140 Quad MS The solvents employed were: A = 0.1% v / v solution of TFA in Water. B = 0.1% v / v solution of TFA in Acetonitrile.

[0345] The gradient employed was: TimeFlow%A%B01mL90102.51mL01004.21mL0100

[0346] The UV detection wavelength (Bandwidth 8): 220nm and 254nm. Injection volume: 1ulMS Conditions

[0347] MS:Agilent 6140 Quad MSIonisation mode:Positive LCMS Method: LCMS Method N

[0348] The LCMS analysis was conducted on an Agilent Zorbax Eclipse XDB-C18 (150mm × 4.6 mm, i.d. 5 µm packing diameter) at Ambient temperature on an Agilent 1200 HPLC with a Model 6140 Quad MS

[0349] The solvents employed were: A = 0.1% v / v solution of TFA in Water. B = 0.1% v / v solution of TFA in Acetonitrile.

[0350] The gradient employed was: TimeFlow%A%B01mL9010121mL0100131mL0100

[0351] The UV detection wavelength (Bandwidth 8): 220nm and 254nm. Injection volume: 1ulMS Conditions

[0352] MS: Agilent 6140 Quad MS Ionisation mode: Positive

[0353] The following abbreviations may be used in this specification: Abbreviation Meaning AcOHacetic acidaq.aqueousBBr 3 boron tribromideBOC, tBOCtert-butoxyca rbonylbrinesaturated aqueous sodium chlorideBuOHbutanolCDCl 3 deuterated chloroformCDI1,1'-carbonyldiimidazoleCH 2 Cl 2 or DCMmethylene chloride or dichloromethaneCH 3 CN or MeCNacetonitrileCH 3 NH 2 methylamineddayDASTdiethylaminosulfur trifluorideDCE1,2-dichloroethaneDIEA or DIPEAdiisopropyl ethylamineDMAdimethylacetamideDMAP4-dimethylaminopyridineDMFN,N-dimethylformamideDMSOdimethylsulfoxideEDC1-ethyl-3-(3-dimethylaminopropyl)carbodiimideequivequivalentsEtethylEt 3 N or TEAtriethylamineEt 2 Odiethyl etherEtOAcethyl acetateEtOHethanolFCCflash column chromatographyh, hrhour(s)HATUO-(7-azabenzotriazol-1yl)-N,N,N',N'-tetramethylyronium hexafluorophosphateHCIhydrochloric acidHOAt1-hydroxy-7-azabenzotriazoleHOBthyd roxybenzotriazoleHPLChigh-performance liquid chromatographyICliodine monochlorideIPAisopropyl alcoholi-Pr 2 NEtN',N'-diisopropylethylamineK 2 CO 3 potassium carbonateKHMDSpotassium bis(trimethylsilyl)amideKOt-Bupotassium tert-butoxideKOHpotassium hydroxideLCMSliquid chromatography-mass spectroscopyLiAlH 4 lithium aluminum hydrideLiHDMSlithium hexamethyldisilazideLiOHlithium hydroxideMemethylMeOH or CH 3 OHmetha nolMgSO 4 magnesium sulfateminminute(s)MSmass spectrumµwmicrowaveNaBH4sodium borohydrideNa 2 CO 3 sodium carbonateNaHCO 3 sodium bicarbonateNaOHsodium hydroxideNa 2 SO 4 sodium sulfateNBSN-bromosuccinimideN 2 H 2 hydrazineNH 4 Clammonium chlorideNH 4 OHammonium hydroxideNiC 2 ·6H 2 Onickel (II) chloride hexahydrateNMPN-methyl-2-pyrrolidoneNMRnuclear magnetic resonancePd / Cpalladium on carbonPhphenylPOCl 3 phosphoryl chloridePSIpound-force per square inchRBround bottomrm or rxn mixturereaction mixturert / RTroom temperaturesatd.saturatedsmstarting materialTBAFtetra-n-butylammonium fluorideTFAtrifluoroacetic acidTHFtetrahydrofuranTMEDAtetra methylethylened ia mi neTMSItrimethylsilyl iodideTMSN 3 trimethylsilyl azideT3P2,4,6-tripropyl-1,3,5,2,4,6-trioxatriphosphorinane-2,4,6-trioxidet R or Rf or Rtretention timeTsOHp-toluenesulfonic acid Step 1: 4-chloro-3-methoxy-5-nitrobenzamide

[0354]

[0355] Methyl 4-chloro-3-methoxy-5-nitrobenzoate (1000 mg, 4.07 mmol) was stirred in NH 4 OH (10 mL, 77 mmol) at RT for 24 h. The reaction temperature was then increased to 50 °C for 2 h. An additional 2 mL (~ 3.7 eq) of NH 4 OH was added to the vessel. After an additional 2 h stirring at 50 °C (4 h total) the reaction was cooled to RT. The solid was filtered and rinsed with cold water. The solid was dried under house vacuum and lyophilized to give 4-chloro-3-methoxy-5-nitrobenzamide (710 mg, 2.99 mmol, 73% yield) as a tan solid. 1< H NMR (400 MHz, DMSO-d 6 ) δ ppm 8.31 (br. s., 1 H), 8.06 (d, J=1.77 Hz, 1 H), 7.88 (d, J=1.77 Hz, 1 H), 7.81 (br. s., 1 H), 4.02 (s, 3 H). LCMS (LCMS Method D): Rt = 0.71 min, [M+H] +< = 230.9.Step 2: 4-chloro-3-hydroxy-5-nitrobenzamide

[0356]

[0357] 4-chloro-3-methoxy-5-nitrobenzamide (1 g, 4.34 mmol) was suspended in dry DCM (15 mL) and stirred at rt. To the reaction was added BBr 3 (17.4 mL, 1M in DCM) dropwise. A slurry rapidly formed which was stirred overnight at rt under nitrogen. The reaction was poured into ice water (300 mL) and stirred vigorously for 30 min. The resulting suspension was filtered and the solids dried to afford the title compound (610 mg, 2.82 mmol, 65% yield). 1< H NMR (400 MHz, DMSO-d 6 ) δ ppm 11.53 (br. s., 1 H), 8.17 (br. s., 1 H), 7.92 (s, 1 H), 7.72 (s, 1 H), 7.66 (br. s., 1 H). LC-MS (LCMS Method D) Rt = 0.60 min, [M+H] +< = 217.Intermediate 24-(5-(5-Carboxy-3-methyl-1H-pyrazol-1-yl)pentyl)-1-ethyl-3-methyl-1H-pyrazole-5-carboxylic acid

[0358] Step 1:Ethyl 3-methyl-1-(5-(trimethylsilyl)pent-4-yn-1-yl)-1H-pyrazole-5-carboxylate

[0359]

[0360] A mixture of ethyl 3-methyl-1H-pyrazole-5-carboxylate (22 g, 143 mmol), (5-chloropent-1-yn-1-yl)trimethylsilane (24.94 g, 143 mmol), K 2 CO 3 (39.4 g, 285 mmol), and DMF (4 mL) was stirred at 60 °C overnight under a nitrogen gas atmosphere. The mixture was then dissolved in DCM and washed with water. The organic phase was dried over anhydrous Na 2 SO 4 , filtered, concentrated under reduced pressure, and purified by column chromatography on silica gel (petroleum ether / EtOAc = 10:1) to afford ethyl 3-methyl-1-(5-(trimethylsilyl)pent-4-yn-1-yl)-1H-pyrazole-5-carboxylate (12.5 g, 42.7 mmol, 30% yield) as a colorless oil. LCMS (LCMS Method A): Rt = 2.43 min, [M+H] +< = 293.Step 2:Ethyl 3-methyl-1-(pent-4-yn-1-yl)-1H-pyrazole-5-carboxylate

[0361]

[0362] A mixture of ethyl 3-methyl-1-(5-(trimethylsilyl)pent-4-yn-1-yl)-1H-pyrazole-5-carboxylate (37.7 g, 129 mmol), K 2 CO 3 (44.5 g, 322 mmol), and EtOH (800 mL) was stirred at rt overnight. The mixture was then filtered and the filtrate was concentrated under reduced pressure. The residue was dissolved in DCM, washed with water, dried over anhydrous Na 2 SO 4 , filtered, and concentrated under reduced pressure to afford ethyl 3-methyl-1-(pent-4-yn-1-yl)-1H-pyrazole-5-carboxylate (20 g, 91 mmol, 70.4% yield) as a colorless oil. LCMS (LCMS Method A): Rt = 2.08 min, [M+H] +< = 221.Step 3:Benzyl 1-ethyl-3-methyl-1H-pyrazole-5-carboxylate

[0363]

[0364] A mixture of 1-ethyl-3-methyl-1H-pyrazole-5-carboxylic acid (20 g, 130 mmol), (bromomethyl)benzene (22.2 g, 130 mmol), K 2 CO 3 (26.9 g, 195 mmol), and DMF (200 mL) was stirred at 60 °C overnight. The mixture was then dissolved in DCM, washed with water, dried over anhydrous Na 2 SO 4 , filtered, concentrated under reduced pressure, and purified by column chromatography on silica gel (petroleum ether / EtOAc = 10:1) to afford benzyl 1-ethyl-3-methyl-pyrazole-5-carboxylate (31.4 g, 129 mmol, 99% yield) as a colorless oil. LCMS (LCMS Method A): Rt = 2.09 min, [M+H] +< = 245.Step 4:Benzyl 1-ethyl-4-iodo-3-methyl-1H-pyrazole-5-carboxylate

[0365]

[0366] A mixture of benzyl 1-ethyl-3-methyl-1H-pyrazole-5-carboxylate (31.6 g, 129 mmol), 1-iodopyrrolidine-2,5-dione (34.9 g, 155 mmol) and DMF (400 mL) was stirred at 90 °C for 2 days. The mixture was then allowed to cool to rt, dissolved in DCM, and washed with a saturated aqueous sodium thiosulfate solution. The organic layer was dried over anhydrous Na 2 SO 4 , filtered, concentrated under reduced pressure, and purified by column chromatography (petroleum ether / EtOAc = 10:1) to afford benzyl 1-ethyl-4-iodo-3-methyl-1H-pyrazole-5-carboxylate (42.6 g, 115 mmol, 89% yield). LCMS (LCMS Method A): Rt = 2.31 min, [M+H] +< = 371.Step 5:Benzyl 4-(5-(5-(ethoxycarbonyl)-3-methyl-1H-pyrazol-1-yl)pent-1-yn-1-yl)-1-ethyl-3-methyl-1H-pyrazole-5-carboxylate

[0367]

[0368] A mixture of ethyl 3-methyl-1-(pent-4-yn-1-yl)-1H-pyrazole-5-carboxylate (10.0 g, 45.4 mmol), benzyl 1-ethyl-4-iodo-3-methyl-1H-pyrazole-5-carboxylate (16.8 g, 45.4 mmol), copper(I) iodide (0.864g, 4.54 mmol), bis(triphenylphosphine)palladium(II) chloride (0.319 g, 0.454 mmol), and Et 3 N (200 mL) was stirred at 60 °C overnight under a nitrogen gas atmosphere. The mixture was then dissolved in DCM and washed with water. The organic phase was dried over anhydrous Na 2 SO 4 , filtered, concentrated under reduced pressure, and purified by column chromatography on silica gel (petroleum ether / EtOAc = 5:1) to afford benzyl 4-(5-(5-(ethoxycarbonyl)-3-methyl-1H-pyrazol-1-yl)pent-1-yn-1-yl)-1-ethyl-3-methyl-1H-pyrazole-5-carboxylate (9.5 g, 20.5 mmol, 45.3% yield) as a yellow solid. LCMS (LCMS Method B): Rt = 2.66 min, [M+H] +< = 463.Step 6:4-(5-(5-(Ethoxycarbonyl)-3-methyl-1H-pyrazol-1-yl)pentyl)-1-ethyl-3-methyl-1H-pyrazole-5-carboxylic acid

[0369]

[0370] A mixture of benzyl 4-(5-(5-(ethoxycarbonyl)-3-methyl-1H-pyrazol-1-yl)pent-1-yn-1-yl)-1-ethyl-3-methyl-1H-pyrazole-5-carboxylate (19.0 g, 41.10 mmol), 10% Pd / C (0.22 g, 2.05 mmol), and THF (500 mL) was stirred at rt under a hydrogen gas atmosphere (4 atm) for 2 days. The reaction mixture was then filtered and concentrated under reduced pressure. The residue obtained was recrystallized from EtOAc / petroleum ether (1:5, v / v) to afford 4-(5-(5-(ethoxycarbonyl)-3-methyl-pyrazol-1-yl)pentyl)-1-ethyl-3-methyl-pyrazole-5-carboxylic acid (10.5 g, 27.90 mmol, 67.9% yield). 1< H-NMR (400 MHz, CDCl 3 ) δ NMR (400 MHz, CDCI, v / v) to afford 4-(5-(5-(ethoxycarbonyl)-3-methyl-pyrazol-1-yl)pentyl)-1-ethyl-3-methyl-pyrazole-5-carboxylic acid (10.5 g, 27.90 mmol, 67.9% yield). 1< H-NMR (400 MHz, CDCl 3 ) δ ppm 6.63 (s, 1H), 4.57-4.48 (m, 4H), 4.38-4.32 (m, 2H), 2.74-2.62 (m, 2H), 2.32 (s, 3H), 2.23 (s, 3H), 1.91-1.86 (m, 2H), 1.59-1.54 (m, 2H), 1.45-1.37 (m, 8H). LCMS (LCMS Method A): Rt = 1.59 min, [M+H] +< = 377.Step 7:4-4-(7-(5-Carboxy-3-methyl-1H-pyrazol-1-yl)heptyl)-1-ethyl-3-methyl-1H-pyrazole-5-carboxylic acid

[0371]

[0372] To a suspension of 4-(5-(5-(ethoxycarbonyl)-3-methyl-1H-pyrazol-1-yl)pentyl)-1-ethyl-3-methyl-1H-pyrazole-5-carboxylic acid (9.0 g, 23.9 mmol) in MeOH (120 mL) and water (120 mL) stirred at rt was added a 2 M aq. NaOH solution (60 mL, 119.5 mmol). The reaction mixture was stirred at rt for 30 min. The mixture was then acidified to pH 4 with the addition of a 6 M HCI solution upon which a solid precipitated from the reaction mixture. The solid was collected by filtration and dried under reduced pressure to afford 4-(5-(5-carboxy-3-methyl-1H-pyrazol-1-yl)pentyl)-1-ethyl-3-methyl-1H-pyrazole-5-carboxylic acid (6.5 g, 18.7 mmol, 78.1% yield) as a white solid. 1< H-NMR (400 MHz, DMSO-d 6 ) δ ppm 6.57 (s, 1H), 4.40-4.34 (m, 4H), 2.53 (t, J = 7.6 Hz, 2H), 2.16 (s, 3H), 2.09 (s, 3H), 1.74-1.67 (m, 2H), 1.44-1.37 (m, 2H), 1.27-1.16 (m, 5H). LCMS (LCMS Method A): Rt = 1.40 min, [M+H] +< = 349.Intermediate 3(3-Bromopropoxy)(tert-butyl)dimethylsilane

[0373]

[0374] To 1H-imidazole (13.4 g, 197 mmol) in DCM (100 mL) was added 3-bromopropan-1-ol (13.7 g, 99 mmol) followed slowly by tert-butylchlorodimethylsilane (17.8 g, 118 mmol) in DCM (20 ml). After 3 hr at RT, the reaction was concentrated to ~100 mL and poured in EtOAc (800 mL), washed with 5% aq citric acid (2 × 200 mL) and brine. The organic layer was dried over MgSO 4 , filtered and concentrated to yield the title compound (10.0 g, 39.5 mmol, 40 % yield). 1< H NMR (400 MHz, chloroform-d) δ ppm 3.78 (t, J=5.70 Hz, 2 H), 3.56 (t, J=6.46 Hz, 2 H), 2.07 (t, J=5.83 Hz, 2 H), 0.94 (s, 9 H), 0.11 (s, 6 H).Intermediate 42,2,3,3- Tetrafluorobutane-1,4-diamine

[0375] Step 1: 2,2,3,3-Tetrafluorobutane-1,4-diyl bis(4-methylbenzenesulfonate)

[0376]

[0377] To 2,2,3,3-tetrafluorobutane-1,4-diol (10.0 g, 61.7 mmol) in pyridine (150 mL) at 0 °C was added 4-methylbenzene-1-sulfonyl chloride (29.4 g, 154 mmol) over 5 min, and then the reaction was heated to 55 °C. After 1 day, the reaction was quenched with ice water, and the resulting solid was collected by filtration, dissolved in DCM (200 mL) and washed with 5 % aq H 2 SO 4 (100 mL X 3). The organic layer was dried over Na 2 SO 4 and concentrated to yield the title compound (27.3 g, 58.0 mmol, 94 % yield) as a white solid. LCMS (LCMS Method A): Rt = 1.750 min, [M+H] +< = 470.9Step 2: 1,4-Diazido-2,2,3,3-tetrafluorobutane

[0378]

[0379] 2,2,3,3-Tetrafluorobutane-1,4-diyl bis(4-methylbenzenesulfonate) (10.0 g, 21.3 mmol) and sodium azide (5.53 g, 85.0 mmol) in DMF (40 mL) was stirred at 110 °C overnight. The reaction was quenched with NaClO(aq) and extracted with DCM (5 mL X 3). The combined organic layers were washed with water (10 mL), dried over Na 2 SO 4 and concentrated to yield the title compound (3.5 g, 16.5 mmol, 78 % yield). LCMS (LCMS Method A): Rt = 1.520 min, [M+H] +< = 213.1Step 3: 2,2,3,3-Tetrafluorobutane-1,4-diamine

[0380]

[0381] To a solution of 1,4-diazido-2,2,3,3-tetrafluorobutane (36.0 g, 170 mmol) in MeOH (350 mL) was added 10 % Pd on carbon (18.1 g, 17.0 mmol). The reaction mixture was stirred at 40 °C under hydrogen (4 atm) for 16 hrs. The mixture was filtered through a pad of Celite, washed with MeOH and the filtrate was concentrated in vacuo to yield the title compound (22.0 g, 124 mmol, 73 % yield). 1< H NMR (400 MHz, chloroform-d) δ ppm 3.12 - 3.37 (m, 4 H), 1.43 (br. s., 4 H).Intermediate 51-ethyl-3-methyl-1H-pyrazole-5-carbonyl isothiocyanate

[0382]

[0383] To a 1L round bottom flask was added 1-ethyl-3-methyl-1H-pyrazole-5-carboxylic acid (25 g, 162 mmol) and DCM (500 mL). To this heterogeneous solution was added DMF (0.1 mL, 1.291 mmol) followed by the slow addition of oxalyl chloride (15.61 mL, 178 mmol). During the addition, bubbling was noticed. After stirring for 1 hr at room temperature, the volatiles were removed under vacuum and the crude was co-evaporated twice with dichloromethane (100 mL each). It was assumed 100% yield and the crude (1-ethyl-3-methyl-1H-pyrazole-5-carbonyl chloride (28.0 g, 162 mmol, 100 % yield)) was used directly as it is in the next reaction.

[0384] To a dry 1L round bottom flask was added KSCN (18.92 g, 195 mmol) and acetone (463 ml). This clear homogenous solution was cooled to 0 °C. After 5 min. stirring at 0 °C, 1-ethyl-3-methyl-1H-pyrazole-5-carbonyl chloride (28 g, 162 mmol) was added as a solution in acetone (25 mL). Once the addition was complete, the reaction was allowed to stir at 0 °C. After 1 min. Additional KSCN was added (~2 g) and the reaction was stirred for an additional 20 min. At this time, hexanes (200 mL) was added to the reaction mixture and the crude heterogeneous solution was concentrated in vacuo to one third of the volume. The process of hexanes addition and concentration was repeated twice (300 mL of Hexanes each). After the last concentration, hexanes (200 mL) were added and the solid was removed by filtration, rinsing with hexanes (100 mL). The resulting clear light yellow filtrate concentrated and purified by chromatography (330g Gold silica column; eluting with 0-20% EtOAc / hexanes). The desired product elutes at ~7% EtOAc / hexanes. The desired fractions were combined and concentrated yielding 1-ethyl-3-methyl-1H-pyrazole-5-carbonyl isothiocyanate (27.5 g, 139 mmol, 86 % yield) as a clear colorless liquid. 1H NMR (400 MHz, chloroform-d) δ ppm 6.77 (s, 1 H), 4.54 (q, J=7.10 Hz, 2 H), 2.34 (s, 3 H), 1.44 (t, J=7.22 Hz, 3 H); LCMS (LCMS Method D): Rt = 1.16 min, [M+H] +< = 196.1. The acylisothiocyanate product degrades over time, and so a ~0.4 M 1,4-dioxane solution was prepared and frozen to avoid / slow decomposition. This solution was thawed and used directly in subsequent reactions.Intermediate 6(E)-1-(4-Aminobut-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-1H-benzo[d]imidazole-5-carboxamide hydrochloride

[0385] Step 1: (E)-tert-Butyl (4-((4-carbamoyl-2-nitrophenyl)amino)but-2-en-1-yl)carbamate

[0386]

[0387] A mixture of 4-fluoro-3-nitrobenzamide (10.0 g, 54.3 mmol), (E)-tert-butyl (4-aminobut-2-en-1-yl)carbamate (10.62 g, 57.0 mmol) and K 2 CO 3 (15.01 g, 109 mmol) in DMSO (200 mL) was stirred at RT overnight. The reaction was poured into water (2000 mL) and stirred for 30 min. The resulting solid was collected by filtration to yield the title compound (18.3 g, 52.2 mmol, 96 % yield). LCMS (LCMS Method A): Rt = 1.38 min, [2M+H] +< = 700.5Step 2: (E)-tert-Butyl (4-((2-amino-4-carbamoylphenyl)amino)but-2-en-1-yl)carbamate

[0388]

[0389] To (E)-tert-butyl (4-((4-carbamoyl-2-nitrophenyl)amino)but-2-en-1-yl)carbamate(18.3 g, 52.2 mmol) in DMF (300 mL) was added stannous chloride dihydrate (58.9 g, 261 mmol). After stirring at RT overnight, the reaction was added to sat aq NaHCO 3 (2000 mL), dropwise, and extracted with EtOAc (5 X 500 mL). The combined organic layers were washed with brine (200 mL), dried over Na 2 SO 4 , filtered and concentrated to yield the title compound (16.5 g, 51.5 mmol, 99 % yield) as a yellow oil. LCMS (LCMS Method A): Rt = 1.275 min, [M-BOC+H] +< = 221.1Step 3: (E)-tert-Butyl (4-(2-amino-5-carbamoyl-1Hbenzo[d]imidazol-1-yl)but-2-en-1-yl)carbamate

[0390]

[0391] A mixture of (E)-tert-butyl (4-((2-amino-4-carbamoylphenyl)amino)but-2-en-1-yl)carbamate (16.5 g, 51.5 mmol) and cyanogen bromide (8.18 g, 77 mmol) in THF (200 mL) was heated to reflux overnight. The reaction was cooled to room temperature, diluted with sat aq NaHCO 3 (500 mL), and extracted with EtOAc (5 X 300 mL). The combined organic layers were washed with brine, dried over Na 2 SO 4 , filtered and concentrated. The residue was purified over silica gel, eluting with 50:1 to 20:1 DCM in MeOH (+ 3 % NH 4 OH) to yield the title compound (13.7 g, 39.7 mmol, 77 % yield) as an off-white solid. LCMS (LCMS Method A): Rt = 1.150 min, [M+H] +< = 346.1Step 4: (E)-tert-Butyl (4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)carbamate

[0392]

[0393] To 1-ethyl-3-methyl-1H-pyrazole-5-carboxylic acid (9.17 g, 59.5 mmol) in DCM (500 mL) at 0 °C was added EDC (20.53 g, 107 mmol) and HOBT (18.22 g, 119 mmol). After 15 min, a mixture of (E)-tert-butyl(4-(2-amino-5-carbamoyl-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)carbamate (13.7 g, 39.7 mmol) in DMF (50 mL) was added, followed by TEA (27.6 mL, 198 mmol). The reaction was warmed to RT, stirred overnight and concentrated. The residue was diluted with water (500 mL) and extracted with ethyl acetate (3 X 300 mL), and the combined organic phases were washed with brine, dried over Na 2 SO 4 , filtered and concentrated. The residue was purified over silica gel, eluting with 50:1 to 20:1 DCM: MeOH to give the crude product, which was washed with DCM (300 mL) and collected by filtration to yield the title compound (14.0 g, 29.1 mmol, 73 % yield) as an off-white solid. 1< H NMR (400 MHz, DMSO-d 6 ) δ ppm 12.84 (s, 1 H), 8.00 - 7.97 (m, 2 H), 7.80 - 7.78 (m, 1 H), 7.49 (d, J=8.4 Hz, 1 H), 7.34 (s, 1 H), 6.95 (t, J=5.5 Hz, 1 H), 6.66 (s, 1 H), 5.73 - 5.65 (m, 2 H), 4.83 (d, J=4.3 Hz, 2 H), 4.62 (q, J=7.0 Hz, 2 H), 3.52 (s, 2 H), 2.18 (s, 3 H), 1.38 - 1.33 (m, 12 H); LCMS (LCMS Method A): Rt = 1.409 min, [M+H] +< = 482.0Step 5: (E)-1-(4-Aminobut-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-1H-benzo[d]imidazole-5-carboxamide hydrochloride

[0394]

[0395] To a suspension of (E)-tert-butyl (4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)carbamate (3.00 g, 6.23 mmol) in dioxane (60 mL) was added 4N HCl in dioxane (15.6 mL, 62.3 mmol), followed by MeOH (15 mL) to dissolve some remaining solid. After 30 min at RT, the reaction mixture became cloudy and was allowed to stir for approximately 3 days. The resulting solid was collected by filtration and washed with DCM to yield the title compound (2.0 g, 4.8 mmol, 77 % yield) as a white solid. 1< H NMR (400 MHz, DMSO-d 6 ) δ ppm 7.97 - 8.09 (br. s., 1 H), 7.82 (d, J=8.11 Hz, 1 H), 7.50 (d, J=8.11 Hz, 1 H), 7.38 (br. s., 1 H), 6.70 (s, 1 H), 5.97 - 6.08 (m, 1 H), 5.68 - 5.80 (m, 1 H), 4.91 (d, J=4.31 Hz, 2 H), 4.60 (q, J=6.67 Hz, 2 H), 3.42 (br. s., 2 H), 2.18 (s, 3 H), 1.36 (t, J=6.97 Hz, 3 H); LCMS (LCMS Method D): Rt = 0.53 min, [M+H] +< = 382.2Intermediate 71-(5-(5-(ethoxycarbonyl)-1-ethyl-3-methyl-1H-pyrazol-4-yl)pentyl)-3-methyl-1H-pyrazole-5-carboxylic acid

[0396] Step 1: benzyl 3-methyl-1H-pyrazole-5-carboxylate

[0397]

[0398] A mixture of 3-methyl-1H-pyrazole-5-carboxylic acid (50 mg, 0.396 mmol) and KHCO 3 (47.6 mg, 0.476 mmol) in DMSO (2 mL) was stirred for 30 min, and (bromomethyl) benzene (0.045 mL, 0.377 mmol) was added. The mixture was stirred for 4 h at RT, diluted with EtOAc (20 mL), washed with water and brine, and dried over Na 2 SO 4 . The mixture was filtered and concentrated, and the residue was purified by column chromatography (Combiflash, 0-50 % EtOAc in hexane) to afford the title compound (66 mg, 0.305 mmol, 77 % yield) as a white solid. 1< H NMR (400 MHz, DMSO-d 6 ) δ ppm 13.19 (br. s., 1 H) 7.34 - 7.48 (m, 5 H) 6.52 (s, 1 H) 5.29 (s, 2 H) 2.27 (s, 3 H). LCMS (LCMS Method D): Rt = 0.86 min, [M+H] +< = 216.9.Step 2: benzyl 3-methyl-1-(pent-4-yn-1-yl)-1H-pyrazole-5-carboxylate

[0399]

[0400] A mixture of DIAD (25.9 mL, 133 mmol) and triphenylphosphine (34.9 g, 133 mmol) in tetrahydrofuran (THF) (600 mL) was stirred for 30 min at 0 °C, and pent-4-yn-1-ol (11.36 mL, 122mmol) was then added. The mixture was stirred for 30 min, and benzyl 3-methyl-1H-pyrazole-5-carboxylate (24 g, 111 mmol) was added. It was allowed to warm to RT and stirred overnight. The reaction was diluted with EtOAc (1000 mL), washed with saturated NaHCO 3 , and brine, dried over Na 2 SO 4 , filtered and concentrated. The oily residue was treated with 10 % EtOAc in hexane (500 mL), and a white precipitate formed. The precipitate was filtered off and washed with 10 % EtOAc in hexane. The combined filtrates were concentrated, and the residue was purified by column chromatography (Combiflash, 0-15 % EtOAc in hexane) to afford the title compound (27.5 g, 97 mmol, 88 % yield) as a white solid. 1< H NMR (400 MHz, CHLOROFORM-d) δ ppm 7.34 - 7.47 (m, 5 H) 6.68 (s, 1 H) 5.33 (s, 2 H) 4.63 (t, J=7.03 Hz, 2 H) 2.30 (s, 3 H) 2.19 - 2.26 (m, 2 H) 2.09 (quin, J=7.09 Hz, 2 H) 1.97 (br. s., 1 H); LCMS (LCMS Method D): Rt = 1.21 min, [M+H] +< = 283.0.Step 3: ethyl 1-ethyl-3-methyl-1H-pyrazole-5-carboxylate

[0401]

[0402] Oxalyl chloride (5.68 ml, 64.9 mmol) was added to a suspension of 1-ethyl-3-methyl-1H-pyrazole-5-carboxylic acid (5 g, 32.4 mmol) in DCM (40 mL) at RT under N 2 and two drops of DMF were added. The mixture was stirred for 2 hours at RT, concentrated and dried in vacuo. Ethanol (50 ml, 856 mmol) was added, and the mixture was stirred for 1 hour at RT. The reaction was concentrated and dried in vacuo to give a light-yellow oil which was taken into EtOAc (100 mL), washed with saturated NaHCO 3 and brine, dried over Na 2 SO 4 , filtered, concentrated and the resulting residue was dried in vacuo to give the title compound (5.5 g, 30.2 mmol, 93 % yield) as a light-yellow oil. 1< H NMR (400 MHz, CHLOROFORM-d) δ ppm 6.63 (s, 1 H) 4.56 (q, J=7.11 Hz, 2 H) 4.35 (q, J=7.11 Hz, 2 H) 2.30 (s, 3 H) 1.44 (t, J=7.28 Hz, 3 H) 1.39 (t, J=7.28 Hz, 3 H). LCMS (LCMS Method E): Rt = 0.81 min, [M+H] +< = 183.1.Step 4: ethyl 1-ethyl-4-iodo-3-methyl-1H-pyrazole-5-carboxylate

[0403]

[0404] A mixture of ethyl 1-ethyl-3-methyl-1H-pyrazole-5-carboxylate (5.5 g, 30.2 mmol) and NIS (8.15 g, 36.2 mmol) in DMF (100 mL) was heated to 90 °C and stirred for 3 days under N 2 . The reaction was cooled to RT, diluted with EtOAc (200 mL), washed with saturated Na 2 S 2 O 3 , 5 % LiCl, and brine, dried over Na 2 SO 4 , filtered, concentrated, and the resulting residue was purified by column chromatography (Combiflash, 0-7 % EtOAc in hexane) ...

Claims

1. A compound which is (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide having the structure of or a tautomer thereof, or a pharmaceutically acceptable salt thereof, or a hydrate thereof.

2. The compound according to claim 1, which is a pharmaceutically acceptable salt of (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide, or a tautomer thereof.

3. The compound according to claim 1, which is (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide, or a tautomer thereof.

4. The compound according to claim 1, which is (E)-1-((E)-4-((E)-5-carbamoyl-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-7-(3-morpholinopropoxy)-2,3-dihydro-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-7-methoxy-2,3-dihydro-1H-benzo[d]imidazole-5-carboxamide having the structure of or a pharmaceutically acceptable salt thereof, or a hydrate thereof.

5. The compound according to claim 4, which is a pharmaceutically acceptable salt of (E)-1-((E)-4-((E)-5-carbamoyl-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-7-(3-morpholinopropoxy)-2,3-dihydro-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-7-methoxy-2,3-dihydro-1H-benzo[d]imidazole-5-carboxamide.

6. The compound according to claim 4, which is (E)-1-((E)-4-((E)-5-carbamoyl-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-7-(3-morpholinopropoxy)-2,3-dihydro-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-7-methoxy-2,3-dihydro-1H-benzo[d]imidazole-5-carboxamide.

7. The compound according to claim 1, which is (Z)-1-((E)-4-((Z)-5-carbamoyl-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-7-(3-morpholinopropoxy)-2,3-dihydro-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-7-methoxy-2,3-dihydro-1H-benzo[d]imidazole-5-carboxamide having the structure of or a pharmaceutically acceptable salt thereof, or a hydrate thereof.

8. The compound according to claim 7, which is a pharmaceutically acceptable salt of (Z)-1-((E)-4-((Z)-5-carbamoyl-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-7-(3-morpholinopropoxy)-2,3-dihydro-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-7-methoxy-2,3-dihydro-1H-benzo[d]imidazole-5-carboxamide.

9. The compound according to claim 7, which is (Z)-1-((E)-4-((Z)-5-carbamoyl-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-7-(3-morpholinopropoxy)-2,3-dihydro-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-((1-ethyl-3-methyl-1H-pyrazole-5-carbonyl)imino)-7-methoxy-2,3-dihydro-1H-benzo[d]imidazole-5-carboxamide.

10. The compound according to claim 1, which is (E)-1-(4-(5-carbamoyl-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-(3-morpholinopropoxy)-1H-benzo[d]imidazol-1-yl)but-2-en-1-yl)-2-(1-ethyl-3-methyl-1H-pyrazole-5-carboxamido)-7-methoxy-1H-benzo[d]imidazole-5-carboxamide tris hydrochloride, or a tautomer thereof.

11. A pharmaceutical composition comprising the compound, a tautomer thereof, a pharmaceutically acceptable salt thereof, or a hydrate thereof according to any one of claims 1-10, and a pharmaceutically acceptable excipient.

12. A compound, a tautomer thereof, a pharmaceutically acceptable salt thereof, or a hydrate thereof according to any one of claims 1-10 for use in therapy.

13. A compound, a tautomer thereof, a pharmaceutically acceptable salt thereof, or a hydrate thereof according to any one of claims 1-10 for use in the treatment of a STING-mediated disease.

14. The compound, a tautomer thereof, a pharmaceutically acceptable salt thereof, or a hydrate thereof for use as defined in claim 13, wherein the disease is cancer.

15. The compound, a tautomer thereof, a pharmaceutically acceptable salt thereof, or a hydrate thereof for use as defined in claim 13, wherein the disease is a solid tumor.

16. The compound, a tautomer thereof, a pharmaceutically acceptable salt thereof, or a hydrate thereof for use as defined in claim 14, wherein the cancer is acute myeloid leukemia (AML).

17. A combination comprising a compound, a tautomer thereof, a pharmaceutically acceptable salt thereof, or a hydrate thereof according to any one of claims 1-10 and an immuno-modulator.

18. The combination as defined in claim 17, wherein the immuno-modulator is an anti-PD-L1 agent.

19. The combination as defined in claim 17, wherein the immuno-modulator is a PD-1 antagonist.

20. The combination as defined in claim 19, wherein the PD-1 antagonist is an anti-PD-1 antibody which is pembrolizumab.