Repellent composition against insects that transmit tropical diseases, in spray form

Abstract

The invention relates to a spray composition for repelling insects that are vectors of a tropical disease, characterized in that it further comprises: microcapsules including a core covered with a membrane, said core containing repellent active ingredients including lavandin essential oil and α-menthane-3,8-diol; a binder; a wetting agent; and water. The invention also relates to a repellent treatment method in which said repellent composition is sprayed onto a portion of a textile material.

Description

[0001] The present invention relates to repellent compositions in spray form against insect vectors of tropical diseases which are intended to treat textile materials.

[0002] The European Centre for Disease Prevention and Control (ECDC) estimates that nearly one billion people die each year from diseases transmitted by mosquito saliva. It is estimated that approximately 700,000 deaths per year worldwide are caused by mosquitoes, which are vectors of tropical diseases. Indeed, 5% of the 3,000 known mosquito species carry pathogens dangerous to humans, such as dengue fever, chikungunya, and malaria.

[0003] Dengue fever and yellow fever are diseases transmitted by mosquitoes of the genus Aedes. The World Health Organization (WHO) has observed a resurgence of the dengue virus in recent years, to which more than 40% of the world's population is exposed. Malaria is transmitted by mosquitoes of the genus Anapholes. These mosquitoes are mostly found in tropical and subtropical areas.

[0004] The increase in these tropical diseases is related to the growing spread of mosquitoes of the genus Aedes aegypti And Aedes albopictus (tiger mosquito), a highly invasive species that also carries chikungunya.

[0005] The progression of the mosquito Aedes The first cases of chikungunya in Europe appeared in 2007 in the Ravenna region of Italy, even though the virus was primarily concentrated in certain areas of Africa, Southeast Asia, and the Indian subcontinent. Since then, the tiger mosquito has been establishing itself further and further in Europe each year and is subject to increased surveillance by health authorities.

[0006] Among all the methods used to combat these tropical diseases transmitted by insects such as mosquitoes, repellent protection is a key strategy. Indeed, repellency prevents insect bites and thus the spread of the aforementioned tropical diseases. Therefore, there is a constant need to improve repellent formulations against insect vectors of tropical diseases.

[0007] Repellent compositions can come in various forms such as sprays, lotions, wipes and sticks for application to the skin to protect it from insects carrying tropical diseases.

[0008] It is also known to impregnate textile materials (e.g., clothing, bedding, mosquito nets, upholstery fabrics) with repellent compositions in order to protect against insects that transmit tropical diseases: either by wearing treated clothing, or by protecting one's environment with a mosquito net or treated bedding or furniture fabric.

[0009] Therefore, various treatments for textile materials have been developed to give them repellent properties against insects that transmit tropical diseases. These treatments consist of impregnating the textile material with a repellent composition, which can, for example, be in the form of a spray containing repellent active ingredients.

[0010] Known repellent compositions in spray form often include repellent active ingredients such as permethrin or essential oils that are solubilized in a solution.

[0011] However, the effect of these treatments, which consist of spraying such repellent compositions on a textile material, is generally very short-lived, lasting from 4 to 8 hours after application, regardless of the use of the textile material (wearing or even storage before use).

[0012] Furthermore, very few active ingredients exhibit satisfactory repellent properties against insect vectors of tropical diseases, such as mosquitoes. US 2009 / 010977 A1 describes repellent nanocapsules. WO 2007 / 085856 A1 and US 2009 / 186096 A1 describe the repellent properties of p-menthane-3,8-diol and lavandin, respectively.

[0013] The present invention remedies all these drawbacks by proposing a new repellent composition against insect vectors of tropical diseases such as mosquitoes in the form of a spray and which is intended to impregnate a textile material to give it a perfectly effective and long-lasting repellent effect over time.

[0014] The inventors of the present invention have indeed developed a new repellent composition in the form of a spray by choosing specific repellent active ingredients which are also micro-encapsulated in such a way that they are released progressively over time, so as to increase the duration of protection of the textile material compared to that of textile materials impregnated with other repellent compositions in the form of a spray.

[0015] More specifically, the microcapsules of the repellent composition according to the invention comprise a core containing at least said repellent active ingredients, said core being coated with a membrane.

[0016] Thus, the present invention has as its primary object a repellent composition against insect vectors of a tropical disease, such as mosquitoes, in the form of a spray which comprises at least: microcapsules comprising a core coated with a membrane, said core containing at least repellent active ingredients which include at least lavandin essential oil and p-menthane-3,8-diol; at least one binding agent; at least one wetting agent; water.

[0017] The microencapsulation of the aforementioned repellent active ingredients offers the advantage of protecting them within the membrane and releasing them only when the membrane bursts. Therefore, these encapsulated active ingredients retain their repellent properties for extended periods.

[0018] The release of the active ingredients contained within the microcapsule core occurs through the rupture of the microcapsule membrane by a mechanical process such as rubbing, friction, shearing, or scratching. For example, this could be friction created when wearing clothing treated with the repellent composition or a piece of bedding and their contact with the skin, or friction of the treated textile material against any contact surface (e.g., furniture, skin).

[0019] Textile materials treated with the repellent composition according to the invention exhibit a lasting repellent effect against insect vectors of tropical diseases, even after several years of storage (at least 3 years) if there has been no rupture of the microcapsule membranes by a mechanical process as detailed above.

[0020] Furthermore, micro-encapsulation allows the fixing of repellent active ingredients onto the textile material via microcapsules.

[0021] Furthermore, due to their microencapsulation, the repellent active ingredients contained in the repellent composition according to the invention have the advantage of being protected against harmful external influences such as light, bacteria, chemical changes in the environment (e.g. pH, presence of water).

[0022] In the context of the present invention, "binding agent" means an agent that enables the microcapsules to adhere to the textile material. Such agents for binding microcapsules to textile materials are perfectly within the capabilities of those skilled in the art.

[0023] In the context of the present invention, "wetting agent" means an agent that ensures the wettability of the repellent composition on the textile material.

[0024] In one embodiment of the invention, the microcapsules comprise, relative to the total mass of the active ingredients they contain: between 5 and 50%, preferably between 5 and 10%, by mass of lavandin essential oil; between 1 and 95%, preferably between 50 and 95%, by mass of p-menthane-3,8-diol.

[0025] In one embodiment of the invention, the core of the microcapsules comprises lemon eucalyptus essential oil. This essential oil contains p-menthane-3,8-diol. Thus, in one embodiment of the invention, the core of the microcapsules comprises at least lemon eucalyptus essential oil and lavandin essential oil.

[0026] In one embodiment of the invention, the core of the microcapsules further comprises at least one compound selected from perfumes, deodorants, skin moisturizers, vitamins, colorants, pigments, antioxidants, acids, bases, bleaching agents, peroxides, adhesives, catalysts, cosmetic oils, plant or algae extracts, softening agents, water repellents, biocidal molecules, insect repellents, heat-insulating agents, flame retardants, and bacteriostatic agents.

[0027] In one embodiment according to the invention, the core of the microcapsules comprises at least one perfumed or deodorizing active ingredient which will be advantageously chosen according to the use of the treated textile material.

[0028] The microcapsule membrane is made of at least one material which can be chosen from gelatin, silicone, polyamides, polyurethanes, polyolefins, proteins, lipids, cellulose and its derivatives, polysaccharides, chitosans, aminoplast resins such as urea-formaldehyde and melamine-formaldehyde, gums, polyacrylates, polystyrenes, gums and polyesters.

[0029] For example, said at least one membrane material may be a polymer selected from polymers such as poly(methylene urea, poly(oxymethylene urea, poly(oxymethylene melamine, polyols, poly(vinyl alcohols, poly(vinylpyrolidones, poly(lactic-co-glycolic acid, polycaprolactones, gums such as acacia, guar, and arabic gums, as well as polymethyl methacrylates.

[0030] Microcapsules can be obtained from any process well known to those skilled in the art, such as a heterogeneous dispersion process in which the active ingredients to be encapsulated are dispersed in a continuous phase (e.g., water) and the membrane material is dispersed so as to be at the interface between said active ingredients and the continuous phase. The membrane material can, for example, be "cured" by cross-linking under appropriate pH and / or temperature conditions, and possibly in the presence of a catalyst. These conditions are known to those skilled in the art.

[0031] Microcapsules can also be manufactured using a gelatin coacervation process.

[0032] Within the framework of the invention, the microcapsules can be made in the form of an aqueous suspension. The microcapsules can, for example, have a solid mass content of between 15 and 50% relative to their total mass.

[0033] In one embodiment of the invention, the microcapsule membrane is an aminoplast resin. This means that the membrane is based on a urea / formaldehyde or melamine / formaldehyde complex. The microcapsules may have been manufactured by polycondensation of urea with formaldehyde, or of melamine (2,4,6-triamino-1,3,5-triazine) with formaldehyde.

[0034] More specifically, in this embodiment, the microcapsules can be obtained in the following manner: A mixture of active ingredients is prepared, comprising at least lavandin essential oil and p-menthane-3,8-diol. This mixture is dispersed in an aqueous solution containing precursors of an aminoplast resin, for example, a formaldehyde / urea or formaldehyde / melamine complex, to obtain a dispersion. An acid catalyst is added to the dispersion to initiate the polycondensation of the aminoplast precursors. A solid and resistant membrane then forms around the droplets of active ingredients.

[0035] The mass of the microcapsule nucleus can be between 2% and 98%, preferably between 70% and 95%, relative to the total mass of said microcapsules.

[0036] The mass of the microcapsule membrane can be between 2% and 98%, preferably between 5% and 30%, relative to the total mass of said microcapsules.

[0037] The average size of the microcapsules can be between 1 and 300 µm, preferably between 1 and 25 µm. The size of the microcapsules depends on the shear stress applied to the mixture during microcapsule formation.

[0038] The binding agent may comprise at least one compound selected from cellulose, cellulose derivatives such as cellulose nitrate and acetate, cationic cellulose derivatives such as those marketed under the trade name UCARE® by Dow, gums such as acacia or lacquer gums, quaternized gums such as quaternized guar gum marketed under the trade name JAGUAR® by Rhodia, polyethyleneimine, modified polyethyleneimine, gelatin, quaternized protein hydrolysates, diallyl dimethyl ammonium chloride / acrylamide polymers such as those marketed under the trade name MERQUAT® by Nalco, vinyl-pyrrolidone and quaternized dimethylaminoalkyl methacrylate copolymers such as those marketed under the trade names GAFQUAT HS 50 and HS 100 by ISP,Polyvinylpyrrolidone and polyvinylcaprolactam polymers, proteins and their derivatives of animal and vegetable origin, waxes (e.g. beeswax, candelilla and carnauba wax), chitosans and their derivatives, resins (e.g. shellac resin), copolymers of polyvinyl methyl ether / maleic anhydride, vinyl acetate / crotonate, vinyl neodecanoate, saturated methylene diphenyl diisothianate, polyperfluoroperhydrophenanthrene, acrylate and acrylamides polymers, polyvinylpyrrolidone-vinyl acetate copolymers.

[0039] The binding agent can also be a dispersion of polyurethane polymers or acrylic copolymers marketed under the trade name ARRISTAN ®< by the company CHT / Bezema.

[0040] Preferably, the binding agent is a polyvinylpyrrolidone-vinyl acetate copolymer.

[0041] The wetting agent may comprise at least one compound selected from ethoxylated fatty alcohols; ethylene oxide ethers formed with C10 to C20 alcohols such as stearylpolyoxyethylene, oleylpolyoxyethylene; ethers formed with alkool phenols such as polyglycol ethers formed from tert-butyl-, octyl- or nonyl-phenol; esters formed with various organic acids such as polyethylene glycol ester of stearic acid or myristic acid, polyethylene glycol oleate; glycerol esters; copolymers of ethylene oxide and propylene oxide; partial esters of fatty or oleic acids formed with hexitol anhydrides such as sorbitol esters formed with oleic acid or stearic acid; tertiary glycols such as 3,6-dimethyl-4-octin-3,6-diol or 4,7-dimethyl-5decine-4,7-diol; polyethylene glycol thioethers such as dodecine mercaptan ether; ethoxylated and / or propoxylated amines; alkyl glucosides;Cationic amine oxides with an amine function or a quaternary ammonium salt; betaines; alkyliminodipropionates.

[0042] The wetting agent may also include at least one compound selected from polyacrylic acid salts, lignosulfonic acid salts, phenolsulfonic or naphthalenesulfonic acid salts, ethylene oxide polycondensates on fatty alcohols or fatty acids or fatty amines, substituted phenols (in particular alkylphenols or arylphenols), sulfosuccinic acid ester salts, taurine derivatives (for example alkyltaurates), phosphoric esters of polyoxyethylated alcohols or phenols, fatty acid and polyol esters, sulfate, sulfonate and phosphate derivatives of the preceding compounds.

[0043] Preferably, the wetting agent is an ethoxylated fatty alcohol.

[0044] The repellent composition may further include at least one preservative agent which is preferably selected from phenoxyethanol, ethylhexylglycerin, benzyl alcohol, methylchloroisothiazolinone, methylisothiazolinone, 2-bromo-2-nitropropane-1,3-diol, methylparaben, ethylparaben, diazolidinyl urea, sodium benzoate, potassium sorbate, 1,3-Bis(hydroxymethyl)-5,5-dimethylimidazolidine-2,4-dione, methylchloroisothiazolinone, methylisothiazolinone, benzoic acid, dehydroacetic acid, polyamine-propyl-biguanidine and methyldibromo glutaronitrile.

[0045] The repellent composition according to the invention may comprise, in relation to the total mass of said composition: between 1 and 30%, preferably between 2.5% and 20%, of microcapsules comprising a core coated with a membrane, said core containing at least repellent active ingredients which include at least lavandin essential oil and p-menthane-3,8-diol; between 0.5% and 50%, preferably between 1% and 10%, of at least one binding agent; between 0.1% and 50%, preferably between 2% and 20%, of at least one wetting agent; optionally between 0.1% and 10%, preferably between 0.1% and 2%, of at least one preservative; q.s. water.

[0046] "Qsp" is the acronym for "Quantity sufficient for" to signify that the mass percentage of water in the composition is such that, added to the percentages of all the other constituents of said composition, a total of 100% is obtained.

[0047] Within the scope of the present invention, the insect vectors of tropical diseases against which the composition according to the invention is repellent may be mosquitoes. For example, these may include the following mosquito species: Aedes aegypti, Culex pipien, Anopheles gambiae. The repellent composition according to the invention can also be effective against mites, lice and bed bugs.

[0048] Within the scope of the present invention, tropical diseases may be dengue fever, yellow fever, chikungunya, and malaria.

[0049] The present invention also relates to a method for treating a textile material to repel insects that are vectors of a tropical disease, characterized in that at least one part of a textile material is sprayed with at least one repellent composition as described above.

[0050] The textile material can be chosen from natural textile materials such as cotton, feather, down, synthetic textile materials such as polyesters, nylon, acrylics or even mixed textile materials.

[0051] In the context of the present invention, the term "textile material" means any textile material, chosen for example from textiles: non-woven (e.g. felts), woven (e.g. warp and weft fabrics), or knitted (e.g., knits), which consists of fibers, filaments or threads, taken alone or in mixtures thereof, which are of natural or synthetic origin or a mixture of natural and synthetic origins thereof.

[0052] By this we mean: Fiber, a thread-like element of a natural or synthetic material of relatively short length; filament, a thread-like element of a natural or synthetic material of infinite or nearly infinite length. Thread, both an elementary thread, namely a unit assembly of fibers resulting for example from spinning, and an assembly of elementary threads or filaments resulting for example from twisting.

[0053] The fibers can be spun, carded, or twisted.

[0054] Natural fibers, filaments or yarns can have a natural plant or animal origin and can thus be chosen from cotton, wool, silk, jute, linen, hemp and viscose.

[0055] Synthetic fibers, filaments or yarns can also have a synthetic origin and be chosen from polyamides, aramids, polyesters, polyacryl-nitrites, aramids, polybenzimidazole, polyether-etherketone, polyphenylene sulfides, polyacrylics, chlorofibers, polyolefins such as polypropylene or polyethylene.

[0056] The textile material treated with the repellent composition according to the invention can be used in various fields such as: bedding (duvets, duvets, pillows, sheets, pillowcases, blankets); clothing; furniture (mosquito nets, rugs, curtains); storage (packing bags for clothes and bedding items such as duvets and blankets or furniture).

[0057] In one embodiment according to the invention, between 0.01 and 5 g / m², preferably between 0.01 and 1 g / m², of a repellent composition according to the invention is sprayed onto said textile material.

[0058] Advantageously, during the treatment process according to the invention, the repellent composition as described above is sprayed so that the textile material is impregnated with at least 0.11 g / m² of p-menthane-3,8-diol and at least 0.01 g / m² of lavandin essential oil.

[0059] Spraying can be done manually or automatically, for example with a mechanical, pneumatic, electric or compressed air sprayer.

[0060] The repellent treatment method according to the invention can be used for domestic purposes. For example, the user sprays the repellent composition on clothing, bedding or furniture, a storage cover, or even a mosquito net to protect it from insects that transmit tropical diseases.

[0061] In another embodiment, the repellent treatment process according to the invention is implemented on an industrial line. The repellent composition according to the invention can thus be sprayed onto a textile material during an industrial process, for example, an industrial process for manufacturing clothing, bedding or furniture, storage bags, or even mosquito nets, so as to treat them before use. As explained above, in the absence of rupture of the microcapsules (for example, by a mechanical process such as friction), the repellent composition according to the invention has the advantage of retaining its repellent effect for several years (at least three years).Thus, textile materials treated, for example during their manufacturing process, with the repellent composition according to the invention can be stored for several years and produce their repellent effect against insect vectors of tropical diseases at the time of their use.

[0062] The textile material can be chosen from clothing, bedding or furniture, storage covers or even mosquito nets.

[0063] The present invention thus relates to a textile material which has been treated by spraying with a repellent composition as described above.

[0064] The textile material can be a textile material as described above.

[0065] This document also applies to a product that has been treated by spraying with a repellent composition as described above, preferably in the quantities described above. The product may be one such product as described above, for example, an item of clothing, bedding or furniture, a storage bag, or a mosquito net. PARTIE EXPERIMENTALE : LE PROTOCOLE EXPERIMENTAL :

[0066] Experimental tests were carried out to measure the repellent effect of a textile material treated with a composition according to the invention against the following three mosquito species: Aedes aegypti ; Culex pipiens ; Anopheles gambiae.

[0067] These were females aged between 5 and 7 days who were deprived of blood food for 12 hours before the tests.

[0068] The trials were conducted on 10 volunteers according to the following experimental protocols: Guidelines for testing mosquito repellent efficacy on human skin (WHO / HTM / NTD / WHOPES / 2009.4), WHO, 2009; Testing Guidelines for Product Performance "Insect Repellent for Application to Human Skin" (OPPTS 810.3700), July 2010 version, U.S. Environmental Protection Agency.

[0069] The 10 volunteers were 5 men aged 20 to 52 years and 5 women aged 24 to 55 years. The area tested was the forearm extending from the wrist to the elbow, namely an average area of ​​600 cm2.

[0070] The repellent composition according to the tested invention comprised, in mass percentages relative to the total mass of said composition: 5% of microcapsules containing by mass relative to the total mass of said capsules: 6.8% of lavandin essential oil and 78.2% of p-menthane-3,8-diol constituting the core of the microcapsules; 15% of a melanin formaldehyde membrane; 3% of a polyvinylpyrrolidone-vinyl acetate copolymer; 4% of an ethoxylated fatty alcohol; 0.5% of a mixture of two preservatives: phenethyl alcohol and ethylhexylglycerin; Qsp of water.

[0071] In a first series of tests, 7.2 g of said repellent composition were sprayed on a size XL cotton t-shirt over its entire surface except the inside, using 8 sprays.

[0072] In a second series of tests, 9 g of the said repellent composition were sprayed onto an XL size cotton t-shirt over its entire surface but not inside, using 10 sprays.

[0073] The test procedure was then identical for the first and second series of tests: The t-shirts were rubbed six times from top to bottom using a flat metal spatula to simulate the friction experienced when wearing the t-shirt, thereby causing the microcapsules to rupture and release the repellent active ingredients they contain. The t-shirts were rubbed at the beginning of the test (i.e., at the initial time), then after 4 hours, 8 hours, and 24 hours.

[0074] The operating conditions were as follows: The tests were carried out in a room with a floor area of ​​12 m² and a volume of 30 m³. The room conditions were: temperature of 25 °C + / - 2°C; 65% + / -5% relative humidity; under air extraction at 30 m 3 < / h.

[0075] The cages containing the mosquitoes had dimensions of 40 cm x 40 cm x 40 cm (i.e. a volume of 64,000 cm3).

[0076] During the tests, the cages were kept under the following conditions: Temperature of 27 °C + / - 2°C; 70% + / -5% relative humidity; Light intensity of 700 lux.

[0077] The number of mosquitoes in each cage was 200 + / - 10 (i.e. an average density of 1 mosquito per 320 cm³).

[0078] Before each test, the volunteers' forearms were washed with unscented soap, then rinsed with water followed by a 70% ethanol solution, and finally dried. Each hand was covered with a vinyl glove.

[0079] Next, one forearm was left uncovered by the treated t-shirt to serve as a control test to check the attractiveness of the volunteer's skin to mosquitoes. This was the so-called "control forearm".

[0080] The other forearm was covered with the fabric of the treated t-shirt, fitted perfectly (over an area of ​​between 450 and 600 cm² depending on the size of the volunteers). This was the so-called "test forearm".

[0081] The tests began 30 minutes after the t-shirt was placed on the forearm and proceeded according to the following steps 1) to 4): 1) Volunteers were positioned facing a cage. 2) The control forearm was placed in the cage for 30 seconds. The test was considered valid only if there were at least 10 landings on the forearm. 3) After validation of the control test, the test forearm (i.e., the forearm covered by the treated t-shirt) was placed in the cage for 3 minutes. 4) The number of landings and mosquito bites on this test forearm were recorded.

[0082] Steps 1) to 4) were then repeated after 4, 8 and 24 hours to monitor the effectiveness of the repellent composition over time.

[0083] Before each test, the t-shirts were rubbed again as described above with a flat metal spatula.

[0084] No side effects such as edema or excessive redness were observed in the volunteers. LES RESULTATS :

[0085] All 10 volunteers' control forearms demonstrated the natural attraction of mosquitoes to their skin. Indeed, in all tests, more than 10 mosquitoes landed on the control forearm within 30 seconds.

[0086] Tables 1 to 6 below detail, for each volunteer, according to the mosquito species considered and the test series (1st or 2nd test series): The number of mosquito bites on the volunteer's test forearm, column "P"; The number of mosquito landings on the volunteer's test forearm, column "A".

[0087] In the tables below, the mention "stop" means that the test was stopped as soon as more than 2 mosquito bites were recorded on the test forearm. Aedes aegypti Table 1 details the results of the first series of tests for the mosquito species Volontaire T = 0 T= 4 h T = 8 h T = 24 h P A P A P A P A 1 0 0 0 0 0 0 stop stop 2 0 0 0 0 0 0 stop stop 3 0 0 0 0 0 0 stop stop 4 0 0 0 1 1 1 stop stop 5 0 0 0 0 0 0 stop stop 6 0 0 0 0 0 0 stop stop 7 0 0 0 0 0 0 stop stop 8 0 0 0 0 0 0 stop stop 9 0 0 0 0 0 0 stop stop 10 0 0 0 1 1 1 stop stop Culex pipiens Table 2 details the results of the first series of tests for the mosquito species Volontaire T = 0 T= 4 h T = 8 h T = 24 h P A P A P A P A 1 0 0 0 0 0 0 stop stop 2 0 0 0 1 1 2 stop stop 3 0 0 0 0 0 1 stop stop 4 0 0 0 0 0 0 stop stop 5 0 0 0 0 0 0 stop stop 6 0 0 0 0 0 0 stop stop 7 0 0 0 0 1 1 stop stop 8 0 0 0 0 1 1 stop stop 9 0 0 0 0 0 0 stop stop 10 0 0 0 0 0 1 stop stop Anopheles gambiae Table 3 details the results of the first series of tests for the mosquito species Volontaire T = 0 T= 4 h T = 8 h T = 24 h P A P A P A P A 1 0 0 0 0 0 1 stop stop 2 0 0 0 0 1 1 stop stop 3 0 0 0 0 0 0 stop stop 4 0 0 0 0 0 0 stop stop 5 0 0 0 1 1 1 stop stop 6 0 0 0 0 0 0 stop stop 7 0 0 0 0 0 2 stop stop 8 0 0 0 0 0 0 stop stop 9 0 0 0 0 1 1 stop stop 10 0 0 0 0 0 0 stop stop Aedes aegypti Table 4 details the results of the -2nd series of tests for the mosquito species Volontaire T = 0 T= 4 h T = 8 h T = 24 h P A P A P A P A 1 0 0 0 0 0 0 0 4 2 0 0 0 0 0 2 0 0 3 0 0 0 0 0 0 0 1 4 0 0 0 0 0 0 0 3 5 0 0 0 0 0 1 0 2 6 0 0 0 0 0 0 0 0 7 0 0 0 1 0 0 0 5 8 0 0 0 0 0 0 0 1 9 0 0 0 0 0 2 0 1 10 0 0 0 0 0 0 0 1 Culex pipiens Table 5 details the results of the 2nd series of tests for the mosquito species Volontaire T = 0 T= 4 h T = 8 h T = 24 h P A P A P A P A 1 0 0 0 0 0 1 0 3 2 0 0 0 0 0 1 0 1 3 0 0 0 0 0 0 0 0 4 0 0 0 1 0 0 0 1 5 0 0 0 0 0 0 0 2 6 0 0 0 0 0 0 0 1 7 0 0 0 0 0 1 0 2 8 0 0 0 0 0 0 0 1 9 0 0 0 0 0 1 0 3 10 0 0 0 0 0 0 0 1 Anopheles gambiae Table 6 details the results of the 2nd series of tests for the mosquito species Volontaire T = 0 T= 4 h T = 8 h T = 24 h P A P A P A P A 1 0 0 0 0 0 2 0 2 2 0 0 0 1 0 1 0 0 3 0 0 0 0 0 0 0 1 4 0 0 0 0 0 0 0 1 5 0 0 0 0 0 1 0 1 6 0 0 0 0 0 0 0 0 7 0 0 0 0 0 0 0 1 8 0 0 0 0 0 3 0 4 9 0 0 0 1 0 1 0 2 10 0 0 0 0 0 0 0 1

[0088] In view of the results, it is noted that the repellent composition according to the invention is perfectly effective in treating a textile material in order to give it repellent properties against mosquitoes, and this in a durable manner.

[0089] Indeed, for the first series of tests (namely 7.2 g of repellent composition according to the invention sprayed on an XL size t-shirt), under these laboratory conditions, the average duration of total effectiveness (i.e. no bites noted) of the t-shirt treated with the repellent composition according to the invention was 4 hours, regardless of the mosquito species.

[0090] After 8 hours, a few bites were observed (less than 2 bites).

[0091] After 24 hours, a significant number of bites were recorded.

[0092] The second series of tests (namely, 9 g of the repellent composition according to the invention sprayed onto an XL-sized t-shirt), under these laboratory conditions, demonstrated total protection against mosquitoes (regardless of the species considered) for a period of 24 hours. After 24 hours of testing, a few landings were observed, but no mosquito bites.

[0093] In addition, a third series of tests was carried out to measure the repellent effect of a textile material treated with a so-called "comparative" composition in relation to the present invention against the mosquito species Aedes aegypti detailed above. These were females aged between 5 and 7 days deprived of blood food 12 hours before this 3rd series of tests.

[0094] The 3rd series of tests was carried out on 10 volunteers according to the experimental protocols detailed above.

[0095] The 10 volunteers were 5 men aged 20 to 52 years and 5 women aged 24 to 55 years. The area tested was the forearm extending from the wrist to the elbow, namely an average area of ​​600 cm2.

[0096] The comparative repulsive composition tested comprised, in mass percentages relative to the total mass of said composition: 5% of microcapsules containing by mass relative to the total mass of said capsules: 6.8% of lavandin essential oil and 78.2% of sunflower oil constituting the core of the microcapsules; 15% of a melanin formaldehyde membrane; 3% of a polyvinylpyrrolidone-vinyl acetate copolymer; 4% of an ethoxylated fatty alcohol; 0.5% of a mixture of two preservatives: phenethyl alcohol and ethylhexylglycerin; Qsp of water.

[0097] Thus, the comparative composition was devoid of one of the essential active principles of the present invention, namely p-menthane-3,8-diol, and was replaced by sunflower oil.

[0098] 9 g of this repellent composition were sprayed on an XL size cotton t-shirt over its entire surface but not inside, using 10 sprays.

[0099] Next, the testing procedure was identical to that detailed above for the 1st and 2nd series of tests.

[0100] No side effects such as edema or excessive redness were observed in the volunteers.

[0101] In Table 7 below, the mention "stop" means that the test was stopped as soon as more than 2 mosquito bites were recorded on the test forearm. Aedes aegypti Table 7 details the results of the 3rd series of tests for the mosquito species Volontaire T = 0 T= 4 h T = 8 h T = 24 h P A P A P A P A 1 stop stop stop stop stop stop stop stop 2 stop stop stop stop stop stop stop stop 3 stop stop stop stop stop stop stop stop 4 stop stop stop stop stop stop stop stop 5 stop stop stop stop stop stop stop stop 6 stop stop stop stop stop stop stop stop 7 stop stop stop stop stop stop stop stop 8 stop stop stop stop stop stop stop stop 9 stop stop stop stop stop stop stop stop 10 stop stop stop stop stop stop stop stop

[0102] Thus, the comparative composition did not show any protection against mosquito bites. Aedes aegypti under laboratory conditions.

[0103] Comparative composition is not at all effective for treating a textile material to give it mosquito-repellent properties.

Claims

1. A repellent composition in a form of spray, characterized in that it comprises at least: - microcapsules comprising a core coated with a membrane, said core containing at least repellent active ingredients which comprise at least lavandin essential oil and p-menthane-3,8-diol; - at least one binding agent; - at least one wetting agent; - water.

2. The repellent composition according to claim 1, characterized in that the microcapsules comprise, relative to the total mass of the active ingredients which they contain: - between 5 and 50%, preferably between 5 and 10%, by mass of lavandin essential oil; - between 1 and 95%, preferably between 50 and 95%, by mass of p-menthane-3,8-diol.

3. The repellent composition according to any one of claims 1 and 2, characterized in that the core of the microcapsules further comprises at least one compound chosen from perfumes, deodorants, skin moisturizers, vitamins, dyes, pigments, antioxidants, acids, bases, bleaching agents, peroxides, adhesives, catalysts, cosmetic oils, plant or seaweed extracts, softening agents, water repellent agents, biocidal molecules, insect repellents, heat-insulating agents, flame retardants and bacteriostatic agents.

4. The repellent composition according to any one of claims 1 to 3, characterized in that the membrane of the microcapsules is made of at least one material chosen from gelatin, silicone, polyamides, polyurethanes, polyolefins, proteins, lipids, cellulose and its derivatives, polysaccharides, chitosans, aminoplast resins such as urea-formaldehyde and melamine-formaldehyde, gums, polyacrylates, polystyrenes and polyesters.

5. The repellent composition according to any one of claims 1 to 4, characterized in that the binding agent is a polyvinylpyrrolidone-vinylacetate copolymer.

6. The repellent composition according to any one of claims 1 to 5, characterized in that the wetting agent is an ethoxylated fatty alcohol.

7. The repellent composition according to any one of claims 1 to 6, <b>characterized in that it comprises, relative to the total mass of said composition: - between 1 and 30%, preferably between 2.5% and 20%, of microcapsules comprising a core coated with a membrane, said core containing at least repellent active ingredients which comprise at least lavandin essential oil and p-menthane-3,8-diol; - between 0.5% and 50%, preferably between 1% and 10%, of at least one binding agent; - between 0.1% and 50%, preferably between 2% and 20%, of at least one wetting agent; - optionally between 0.1% and 10%, preferably between 0.1% and 2%, of at least one preserving agent; - qsp water.

8. A method for the repellent treatment of insect vectors of a tropical disease, mites, lice or bed bugs, of a textile material, characterized in that at least one repellent composition according to any one of claims 1 to 7 is sprayed on at least a part of a textile material.

9. The repellent treatment method according to claim 8, characterized in that between 0.01 and 5 g / m2, preferably between 0.01 and 1 g / m2, of repellent composition according to any one of claims 1 to 7 is sprayed on said textile material.

10. The repellent treatment method according to any one of claims 8 and 9, characterized in that said textile material is chosen from clothing, parts of bedding or furniture, storage covers and mosquito nets.