Genetically engineered glycosyltransferases and methods for glycosylation of steviol glycosides

Abstract

The present invention provides genetically engineered glucosyltransferase (GT) enzymes, polypeptides having GT activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides; the present invention provides genetically engineered sucrose synthase (SuS) enzymes, polypeptides having SuS activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides; the present invention also provides compositions comprising the GT enzymes and methods of using the genetically engineered GT enzymes to produce products with β-glucose linkages; the present invention further provides compositions and methods for the production of rebaudiosides (e.g., rebaudioside M, rebaudioside A, rebaudioside I, and rebaudioside D).The present invention also provides compositions comprising the SuS enzymes and methods for using them; methods for producing the GT and SuS enzymes are also provided.