ELITE EVENT EE-GH7 AND METHODS AND KITS FOR THE IDENTIFICATION OF SAID EVENT IN BIOLOGICAL SAMPLES
Patent Information
- Authority / Receiving Office
- MX · MX
- Patent Type
- Patents
- Current Assignee / Owner
- BASF AGRICULTURAL SOLUTIONS SEED US LLC
- Filing Date
- 2018-10-19
- Publication Date
- 2026-05-19
AI Technical Summary
Existing technologies fail to provide a transgenic cotton plant with dual herbicide tolerance to glyphosate and HPPD inhibitors while maintaining acceptable agronomic performance, and there is a need for a rapid and unambiguous method to identify the Elite Event EE-GH7 in biological samples.
A transgenic cotton plant with an expression cassette containing the 2mEPSPS and hppdPf-W336-1 Pa genes conferring tolerance to both glyphosate and HPPD inhibitors, combined with a PCR-based identification method using specific primers targeting the 5' and 3' flanking regions of EE-GH7 to ensure accurate detection.
The method allows for the unambiguous identification of EE-GH7 in biological samples and ensures that the transgenic cotton plants maintain agronomic performance comparable to non-transgenic isogenic lines, providing dual herbicide tolerance without adverse effects.
Abstract
Description
ELITE EVENT EE-GH7 AND METHODS AND KITS FOR THE IDENTIFICATION OF SAID EVENT IN BIOLOGICAL SAMPLES FIELD OF INVENTION This invention relates to novel nucleic acids and transgenic cotton plants, plant material, and seeds, characterized by harboring a specific transformation event, particularly the presence of genes encoding proteins that confer herbicide tolerance, at a specific location in the cotton genome. The cotton plants of the present invention combine the herbicide tolerance phenotype with agronomic performance, genetic stability, and functionality in different genetic backgrounds equivalent to the corresponding gene pool of non-transformed cotton in the absence of herbicide(s). This invention further provides methods and kits for identifying the presence of plant material specifically comprising the EE-GH7 transformation event in biological samples. BACKGROUND OF THE INVENTION The phenotypic expression of a transgene in a plant is determined by both the structure of the gene or genes themselves and their location within the plant's genome. Furthermore, the presence of transgenes, or foreign DNA, at different locations within the genome will influence the plant's overall phenotype in different ways. Successfully introducing a commercially desirable trait into a plant through genetic manipulation, from an agronomic or industrial perspective, can be a lengthy process dependent on various factors. The transformation and regeneration of genetically transformed plants are only the first steps in a series of selection processes, which include extensive genetic characterization, introgression, and evaluation in field trials, ultimately leading to the selection of an elite event. The unambiguous identification of an Elite Event is increasingly important in light of discussions on novel foods, the segregation of GMO and non-GMO products, and the identification of proprietary material. Ideally, such an identification method should be rapid and simple, without requiring a highly sophisticated laboratory. Furthermore, the method should provide results that allow for the unambiguous determination of the Elite Event without expert interpretation, but which can be subjected to expert scrutiny if necessary. The specific tools for identifying the Elite Event EE-GH7 in biological samples are described herein. In this invention, EE-GH7 has been identified as a Elite event of a transgenic cotton plant population in the development of herbicide-tolerant cotton (Gossypium hirsutum), comprising a gene conferring tolerance to glyphosate combined with a gene conferring tolerance to 4-hydroxyphenylpyruvate dioxygenase (HPPD) inhibitors, each under the control of a plant-expressible promoter. Planting EE-GH7 cotton varieties with double herbicide tolerance provides the ML / a / ZUZZ / UU l U14 Farmers have new options for weed control using Isoxaflutol (IFT) or glyphosate herbicide. Glyphosate is widely used in cotton and other agricultural production systems. The herbicide IFT offers an alternative weed control option to help cotton growers manage problem weed species and as an alternative action tool to reduce the spread of herbicide-resistant weeds. With IFT, a new mode of action is introduced in cotton that is effective against many weeds currently present in cotton fields. Cotton plants comprising a herbicide tolerance gene have been described in the art. WO2007 / 017186 describes a glyphosate-tolerant cotton Elite Event comprising an epsps gene. WO2013 / 026740 describes cotton plants comprising both an hppd gene and an epsps gene that confer tolerance in the greenhouse and in the field. WO2013 / 026740 also describes cotton plants comprising both an hppd gene and an epsps gene, which are introduced into the 3' flanking region of the Elite Event described in WO2008 / 151780 that comprises an insect resistance gene. However, none of the above technique descriptions teaches or suggests an Elite Event comprising both a gene encoding for glyphosate tolerance and a gene conferring tolerance to HPPD inhibitors, and being able to be used flexibly with or without a gene conferring insect resistance. It is well known in the field that it is not at all easy to obtain an Elite Transformation Event tolerant to a commercial herbicide in cotton plants with acceptable agronomic performance, and that provides sufficient tolerance to the herbicide, in particular to 2 different classes of herbicides. SUMMARY OF THE INVENTION The present invention relates to a transgenic cotton plant, or a seed, cells, or tissues thereof, comprising, stably integrated into its genome, an expression cassette comprising a herbicide tolerance gene comprising the coding sequence of the 2mEPSPS gene and another herbicide tolerance gene comprising the coding sequence of the hppdPf-W336-1 Pa gene (as described in Example 1.1 herein and as depicted in SEQ ID NO: 1), which exhibits tolerance to glyphosate and to an HPPD-inhibiting herbicide such as isoxaflutol and, in the absence of herbicide(s), exhibits an agronomic performance that is substantially equivalent to the non-transgenic isogenic line. After the application of one or more herbicides to which tolerance is provided, the plant will have a superior agronomic phenotype compared to a non-transgenic plant. According to the present invention, the cotton plant or seed, cells or tissues thereof comprise the Elite Event EE-GH7. Specifically, the present invention relates to a transgenic cotton plant, seed, cells or tissues thereof, whose genomic DNA is characterized by the fact that, when analyzed ML / a / ZUZZ / UU, incorporated into a PCR Identification Protocol as described herein, utilizes two primers targeting the 5' or 3' flanking region of EE-GH7, and foreign DNA comprising herbicide tolerance genes, respectively, producing a fragment that is specific for EE-GH7. The primers can be directed against the 3' flanking region within SEQ ID NO: 1 and against foreign DNA comprising herbicide tolerance genes, respectively. Primers can also be directed against the 5' flanking region within SEQ ID NO: 1 and against foreign DNA comprising herbicide tolerance genes, respectively, such as primers comprising or consisting (essentially) of the nucleotide sequence of SEQ ID NO: 3 and SEQ ID NO: 4, or SEQ ID NO: 5 and SEQ ID NO: 6 respectively, and produce a DNA fragment of between 50 and 1000 bp, such as a fragment of approximately 126 bp or approximately 120 bp.The reference seed comprising the Elite Event of the invention has been registered with the ATCC under accession number PTA-122856. An embodiment of the invention is the seed comprising Elite Event EE-GH7 under accession number PTA-122856, which, when introduced into a cotton plant, will confer tolerance to herbicides, particularly glyphosate or HPPD inhibitors such as isoxaflutol. An embodiment of the invention is Elite Event EE-GH7 as contained in the seed registered under accession number PTA-122856, which, when introduced into a cotton plant, will provide resistance to herbicides, particularly HPPD inhibitors such as isoxaflutol and glyphosate.This invention includes minor variants of this event, such as a cotton event with tolerance to HPPD inhibitors and tolerance to glyphosate having a nucleotide sequence with at least 90%, at least 95%, at least 98%, at least 99%, at least 99.5%, or at least 99.9% sequence identity with the EE-GH7 nucleotide sequence contained in the seed submitted to the ATCC under entry number PTA-122856, or a cotton event with HPPD inhibitor tolerance and glyphosate tolerance having a nucleotide sequence that differs by 1 to 200, 1 to 150, 1 to 100, 1 to 75, 1 to 50, 1 to 30, 1 to 20, 1 to 10, or 1 to 5 nucleotides from the EE-GH7 nucleotide sequence, as stated in the seed submitted to the ATCC under entry number PTA-122856, or having a nucleotide sequence that differs by 1 to 200, 1 to 150, 1 to 100, 1 to 75, 1 to 50, 1 to 30, 1 to 20, 1 to 10, or 1 to 5 nucleotides of the nucleotide sequence of SEQ ID NO: 1. In one embodiment, EE-GH7 comprises a nucleotide sequence with at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or at least 99.9% sequence identity with the sequence of SEQ ID NO: 1.The seed of deposit number ATCC PTA-122856 is a seed lot consisting of at least approximately 95% transgenic seeds homozygous for the transferred DNA, comprising the Elite Event of the invention, which will grow into herbicide-tolerant plants, being plants tolerant to glyphosate or isoxaflutol. The seed or the seed of the progeny obtainable or obtained from the deposited seed (e.g., after crossing with other cotton plants with a different gene pool) may be sown, and the growing plants may be treated with glyphosate or isoxaflutol as described herein to obtain 100% isoxaflutol- or glyphosate-tolerant plants, which comprise the Elite Event of the invention.The invention further relates to cells, tissues, progeny, and offspring of a plant comprising the Elite Event of the invention, which grows from the seed deposited in the ATCC with accession number PTA-122856. The invention further relates to plants obtainable by propagation or reproduction with a cotton plant comprising the Elite Event of the invention (such as a plant grown from the seed deposited in the ATCC with accession number PTA-122856). The invention also relates to cotton plants comprising Elite Event EE-GH7. The invention further relates to a method for identifying a transgenic plant, or cells or tissues thereof, comprising the Elite Event EE-GH7. This method is based on identifying the presence of characteristic DNA sequences or amino acids encoded by said DNA sequences in the transgenic plant, cells, or tissues. According to a preferred embodiment of the invention, such characterizing DNA sequences are 15 bp or at least 15 bp, preferably 20 bp or at least 20 bp, with a length of 30 bp or more being more preferable. These sequences comprise the insertion site of the event; that is, they are part of the inserted foreign DNA comprising herbicide tolerance genes and a portion of the cotton genome (either the 5' or 3' flanking region) contiguous with it, thus enabling the specific identification of the Elite Event.The invention also relates to plants comprising the EE-GH7 event as identified herein. The present invention further relates to methods for identifying the Elite Event EE-GH7 in biological samples, such methods being based on primers or probes that specifically recognize the 5' or 3' flanking sequence of the foreign DNA comprising the herbicide tolerance genes in EE-GH7. This document also includes any other method for identifying EE-GH7, for example, for identifying its specific characterization sequences, such as whole or partial (targeted) genome sequencing. Specifically, the invention relates to a method comprising amplifying a nucleic acid sequence present in biological samples, using a polymerase chain reaction with at least two primers, one of which recognizes the 5' or 3' flanking region of the foreign DNA comprising the herbicide tolerance genes in EE-GH7, while the other recognizes a sequence within the foreign DNA comprising the herbicide tolerance genes, preferably to obtain a DNA fragment of between 50 and 1000 bp. The primers can recognize a sequence within the 5' flanking region of EE-GH7 (SEQ ID NO: 1, from position 1 to position 1217) or within the 3' flanking region of EE-GH7 (complement to SEQ ID NO: 1 from position 8033 to position 9328) and a sequence within foreign DNA comprising herbicide tolerance genes (SEQ ID NO: 1 from position 1218 to 8032 or its complement), respectively.The primer that recognizes the 5' flanking region may comprise the nucleotide sequence of SEQ ID NO: 3 or SEQ ID NO: 5, and the primer that recognizes a sequence within foreign DNA comprising herbicide tolerance genes may comprise the nucleotide sequence of SEQ. ID NO: 4 or SEQ ID NO: 6 described herein. This invention also relates to the specific primers and the specific DNA amplified using such primers, as described herein. The present invention relates specifically to a method for identifying the Elite Event EEGH7 in biological samples, which comprises amplifying a nucleic acid sequence present in a biological sample using a polymerase chain reaction with two primers comprising or consisting (essentially) of the nucleotide sequences of SEQ ID NO: 3 and SEQ ID NO: 4 respectively, to obtain a DNA fragment of approximately 126 bp, or with two primers comprising or consisting (essentially) of the nucleotide sequences of SEQ ID NO: 5 and SEQ ID NO: 6 respectively, to obtain a DNA fragment of approximately 120 bp. Also included in this invention are the plants comprising the Elite Event EE-GH7 identified in this manner. The present invention further relates to the specific flanking sequences of EE-GH7 described herein, which can be used to develop specific identification methods for EE-GH7 in biological samples. Such specific flanking sequences can also be used as reference control material in identification assays. In particular, the invention relates to the 5' or 3' flanking regions of EE-GH7 that can be used for the development of specific primers and probes as described later herein. Nucleic acid molecules are also suitable as reference material, preferably having a length of 150-850 bp and comprising the sequence that can be amplified by primers comprising or consisting (essentially) of the nucleotide sequence of SEQ ID NO: 3 and SEQ ID NO: 4 or SEQ ID NO: 5 and SEQ ID NO: 6. The invention also relates to methods for identifying the presence of EE-GH7 in biological samples based on the use of such specific primers or probes.The primers may comprise or consist essentially of a nucleotide sequence of 17 to approximately 200 consecutive nucleotides selected from the nucleotide sequence of SEQ ID NO: 1 from nucleotide 1 to nucleotide 1217 or the complement of the nucleotide sequence of SEQ ID NO: 1 from nucleotide 8033 to nucleotide 9328, combined with primers comprising or consisting essentially of a nucleotide sequence of 17 to approximately 200 consecutive nucleotides selected from the nucleotide sequence of SEQ ID NO: 1, such as a nucleotide sequence of 17 to approximately 200 consecutive nucleotides selected from the complement of the nucleotide sequence of SEQ ID NO: 1 from nucleotide 1218 to nucleotide 8032 or the nucleotide sequence of SEQ ID NO: 1 from nucleotide 1218 to nucleotide 8032.Primers may also comprise these nucleotide sequences located at their 3' end, and further comprise unrelated sequences or sequences derived from the aforementioned nucleotide sequences, but comprising mismatched pairings. The invention also relates to kits for identifying the Elite Event EE-GH7 in samples ML / a / ZUZZ / UU fufó biological, said kits comprising at least one primer or probe that specifically recognizes the 5' 3' flanking region of the foreign DNA comprising herbicide tolerance genes in EE-GH7. The kit of the invention may comprise, in addition to a primer that specifically recognizes the 5' or 3' flanking region of EE-GH7, a second primer that specifically recognizes a sequence within foreign DNA comprising herbicide tolerance genes of EE-GH7 for use in a PCR identification protocol. The kits of the invention may comprise at least two specific primers, one of which recognizes a sequence within the 5' flanking region of EE-GH7 or a sequence within the 3' flanking region of EE-GH7, and the other recognizes a sequence within foreign DNA comprising herbicide tolerance genes.The primer that recognizes the 5' flanking region may comprise the nucleotide sequence of SEQ ID NO: 3 and the primer that recognizes transgenes or foreign DNA comprising herbicide tolerance genes may comprise the nucleotide sequence of SEQ ID NO: 4, or the primer that recognizes the 5' flanking region may comprise the nucleotide sequence of SEQ ID NO: 5 and the primer that recognizes transgenes or foreign DNA comprising herbicide tolerance genes may comprise the nucleotide sequence of SEQ ID NO: 6, or any other primer or combination of primers as described herein.The kit may further comprise a probe that recognizes a sequence between the primer that recognizes the 5' flanking region and the primer that recognizes the sequence within the foreign DNA, or that recognizes a sequence between the primer that recognizes the 3' flanking region and the primer that recognizes the sequence within the foreign DNA, such as a probe comprising the sequence SEQ ID NO: 7. The invention further relates to a kit for identifying the Elite Event EE-GH7 in biological samples, said kit comprising PCR primers consisting (essentially) of the nucleotide sequences of SEQ ID NO: 3 and SEQ ID NO: 4, or of the nucleotide sequences of SEQ ID NO: 5 and SEQ ID NO: 6 for use in the EE-GH7 PCR Identification Protocol described herein. Said kit comprising primers consisting (essentially) of the nucleotide sequences of SEQ ID NO: 5 and SEQ ID NO: 6, may further comprise a probe comprising or consisting (essentially) of the nucleotide sequence of SEQ ID NO: 7. The invention also relates to a kit for identifying the Elite Event EE-GH7 in biological samples, the kit comprising a specific probe that essentially comprises a sequence that corresponds to (or is complementary to) a sequence exhibiting between 80% and 100% sequence identity with a specific region of EE-GH7. Preferably, the probe sequence corresponds to a specific region comprising part of the 5' or 3' flanking region of EE-GH7. Even more preferable, the specific probe essentially comprises (or is complementary to) a sequence exhibiting between 80% and 100% sequence identity with the sequence between nucleotide 1197 and nucleotide 1238 of SEQ ID NO: 1, or a sequence exhibiting between 80% and 100% sequence identity with the sequence between nucleotide 8012 ΜΛ / a / ZUZZ / UU was 8053 of SEQ ID NO: 1. According to another aspect of the invention, DNA sequences comprising the insertion site of the event and a sufficient length of polynucleotides from both the cotton genomic DNA and the foreign DNA comprising herbicide tolerance genes (transgene) are described, which can be used as primers or screening tests for EE-GH7, as well as for characterizing plants comprising the EE-GH7 event. Such sequences may comprise at least 9 nucleotides from the cotton genomic DNA and a similar number of nucleotides from the foreign DNA comprising the EE-GH7 herbicide tolerance genes, on each side of the attachment site, respectively. Preferably, such DNA sequences comprise at least 9 nucleotides from cotton genomic DNA and a similar number of nucleotides from foreign DNA comprising herbicide tolerance genes contiguous with the insertion site in SEQ ID NO: 1. In one aspect of the invention, cotton plants comprising such specific DNA sequences are provided. The methods and kits covered by the present invention can be used for various purposes, including, but not limited to, the following: to identify the presence of, or determine the (lower) threshold of, EE-GH7 in plants, plant material, or products such as, but not limited to, food or feed products (fresh or processed) comprising or derived from plant material. Alternatively, the methods and kits of the present invention can be used to identify transgenic plant material for the purpose of segregating between transgenic and non-transgenic materials. Alternatively, the methods and kits of the present invention can be used to determine the quality (i.e., the percentage of pure material) of plant material comprising EE-GH7. The invention also relates to the 5' or 3' flanking regions of EE-GH7, as well as to specific primers and probes developed from the 5' or 3' flanking sequences of EE-GH7. The invention also relates to genomic DNA obtained from plants comprising the Elite Event EE-GH7. This genomic DNA can be used as a reference control material in the identification assays described herein. Also provided herein is a herbicide-tolerant transgenic cotton plant, or cells, parts, seeds, or progeny thereof, each comprising at least one Elite Event. Such Elite Event comprises foreign DNA comprising: i) A first chimeric gene comprising a modified epsps gene from Zea mays encoding a glyphosate-tolerant EPSPS enzyme under the control of a plant-expressible promoter. i) A second chimeric gene comprising a modified hppd gene from Pseudomonas fluorescens encoding an HPPD-inhibiting herbicide-tolerant enzyme under the control of a plant-expressible promoter. In one embodiment, said Elite Event comprises nucleotides 1 to 1217 of SEQ ID NO: 1 immediately upstream contiguous with said foreign DNA and nucleotides 8033 to 9328 of SEQ ID NO: 1 immediately downstream and contiguous with said foreign DNA. In a further embodiment, said Elite Event can be obtained by breeding with a cotton plant grown from reference seeds comprising said event that has been entered into the ATCC with reference number PTA-122856. In another embodiment, the genomic DNA of said cotton plant, or cells, parts, seeds, or progeny thereof, when analyzed by the elite event identification protocol for said event with two primers comprising the nucleotide sequence of SEQ ID NO: 3 and SEQ ID NO: 4 respectively, yields a DNA fragment of (approximately) 126 bp. This document also provides a method for identifying a transgenic cotton plant, or cells, parts, seeds, or progeny thereof tolerant to glyphosate or an HPPD-inhibiting herbicide such as isoxaflutol, in biological samples, said method comprising the amplification of a DNA fragment between 50 and 150 bp from a nucleic acid present in biological samples using a polymerase chain reaction with at least two primers, one of which recognizes the 5' flanking region of the Elite Event specified above.The said 5' flanking region comprises the nucleotide sequence of SEQ ID No. 1 from nucleotide 1 to nucleotide 1217, or the 3' flanking region of said Elite Event, said 3' flanking region comprising either the nucleotide sequence of the complement of SEQ ID No. 1 from nucleotide 8033 to nucleotide 9328, the other primer of said primers recognizing a sequence within foreign DNA comprising the nucleotide sequence of the complement of SEQ ID No. 1 from nucleotide 1218 to nucleotide 8032 or the core elite sequence of SEQ ID No. 1 from nucleotide 1218 to nucleotide 8032. This document also provides a kit for identifying a transgenic cotton plant, as well as cells, parts, seeds, or progeny thereof tolerant to glyphosate or an HPPD-inhibiting herbicide, such as isoxaflutol, in biological samples, said kit comprising a primer that recognizes the 5' flanking region of the Elite Event specified above, said 5' flanking region comprising the nucleotide sequence of SEQ ID NO: 1 from nucleotide 1 to nucleotide 1217, or a primer that recognizes the 3' flanking region of said Elite Event, said 3' flanking region comprising the nucleotide sequence of the complement of SEQ ID NO: 1 from nucleotide 8033 to nucleotide 9328, and a primer that recognizes a sequence within foreign DNA.Said foreign DNA comprises the nucleotide sequence of the complement of SEQ ID NO: 1 from nucleotide 1218 to nucleotide 8032 or the nucleotide sequence of SEQ ID NO: 1 from nucleotide 1218 to nucleotide 8032. In one embodiment of the present invention, the foreign DNA from the Elite Event EE-GH7, as used herein, comprises the nucleotide sequence of SEQ ID NO: 1 from nucleotide 1218 to nucleotide 8032 or its complement, or comprises a sequence having at least 95, 98, 99, or 99.5% sequence identity to the nucleotide sequence of SEQ ID NO: 1 from nucleotide position 1218 to nucleotide position 8032 or its complement. A cotton plant, plant cell, tissue, or seed is also provided, comprising in IVIA / a / 2U22 / UU l U13 its genome a nucleic acid molecule comprising a nucleotide sequence with at least 97, 98, or at least 99% sequence identity with the nucleotide sequence of SEQ ID NO: 1 from nucleotide position 1218 to nucleotide position 8032 or the complement thereof, or a nucleotide sequence with at least 97, 98, or at least 99% sequence identity with SEQ ID NO: 1 or the complement thereof. An embodiment of this invention provides a cotton plant, plant cell, tissue or seed, comprising in its genome a nucleic acid molecule that hybridizes to the nucleotide sequence SEQ ID NO: 1 from nucleotide position 1218 to nucleotide position 8032 or its complement, or hybridizes to the nucleotide sequence of SEQ ID NO: 1 or its complement. Also provided herein is an isolated nucleic acid molecule comprising a nucleotide sequence having at least 99% sequence identity with the nucleotide sequence of SEQ ID NO: 1 from nucleotide position 1218 to nucleotide position 8032 or its complement, or a nucleotide sequence having at least 99% sequence identity with SEQ ID NO: 1 or its complement, or an isolated nucleic acid molecule comprising a nucleotide sequence that hybridizes to the nucleotide sequence of SEQ ID NO: 1 from nucleotide position 1218 to nucleotide position 8032 or its complement, or that exhibits hybridization to the nucleotide sequence of SEQ ID NO: 1 or its complement. Other embodiments according to the invention are summarized in the following paragraphs: 1. A nucleic acid molecule comprising a nucleotide sequence essentially similar to SEQ ID NO: 1 from nucleotide 1207 to nucleotide 1228 or SEQ ID NO: 1 from nucleotide 8022 to 8043, or the complement of such sequences. 2. A nucleic acid molecule comprising a nucleotide sequence essentially similar to SEQ ID NO: 1 from nucleotide 1197 to nucleotide 1238 or SEQ ID NO: 1 from nucleotide 8012 to 8053, or the complement of such sequences. 3. A nucleic acid molecule comprising a nucleotide sequence essentially similar to SEQ ID NO: 1 or the complement of said sequence. 4. A nucleic acid molecule comprising a nucleotide sequence with at least 99% sequence identity to the nucleotide sequence of SEQ ID NO: 1 or its complement. 5. A nucleic acid molecule comprising a nucleotide sequence that hybridizes to the nucleotide sequence of SEQ ID NO: 1 or its complement. 6. Cotton genomic DNA comprising the nucleic acid molecule of any of paragraphs 1 to 5. 7. Cotton genomic DNA comprising the Elite Event EE-GH7. 8. A chimeric DNA comprising foreign DNA, wherein the sequence of said DNA ML / a / ZUZZ / UU foreign fufó consists of the sequence SEQ ID NO: 1 from nucleotide 1218 to nucleotide 8032, flanked by a 5' region and a 3' region, wherein the 5' flanking region immediately upstream and contiguous with said foreign DNA is characterized by a sequence consisting of the sequence SEQ ID NO: 1 from nucleotide 1 to nucleotide 1217, and wherein the 3' flanking region immediately downstream and contiguous with said foreign DNA is characterized by a sequence consisting of the sequence SEQ ID NO: 1 from nucleotide 8033 to 9328. 9. The nucleic acid molecule, or genomic DNA, or chimeric DNA of any of paragraphs 1 to 8, which is an isolated nucleic acid molecule, isolated genomic DNA, or isolated chimeric DNA. 10. A cotton plant, cell, part, tissue, seed or progeny thereof, comprising the nucleic acid molecule of any of paragraphs 1 to 5 or the chimeric DNA of paragraph 8. 11. A transgenic cotton plant, cell, part, tissue, seed or progeny thereof, each of which comprises the Elite Event EE-GH7 in its genome, the reference seed comprising said event being deposited in the ATCC with deposit number PTA-122856. 12. The transgenic cotton plant, cell, part, tissue, seed or progeny thereof, of paragraph 11, whose genomic DNA, when analyzed using the Elite Event Identification Protocol for EE-GH7 with two primers comprising the nucleotide sequence of SEQ ID 3 and SEQ ID 4, respectively, yields a DNA fragment of approximately 126 bp. 13. Seed comprising the Elite Event EE-GH7 entered into the ATCC with the registration number PTA-122856, or its derivatives. 14. A cotton plant, cell, part, tissue, seed, or progeny thereof comprising the Elite Event EE-GH7 obtainable from the seed of paragraph 13. 15. A cotton plant, cell, part, tissue, seed or progeny thereof, each of which includes the Elite Event EE-GH7 in its genome, obtainable by propagation or reproduction of a cotton plant grown from the seed deposited in the ATCC under deposit number PTA122856. 16. A cotton seed comprising the Elite Event EE-GH7, reference seed comprising said event which has been entered into the ATCC with registration number PTA-122856. 17. A transgenic cotton plant, cell, part, tissue, seed or progeny thereof, comprising the Elite Event EE-GH7, which can be obtained from the seed of paragraph 16. 18. A cotton plant, cell, part, tissue, seed, or progeny thereof, comprising in its genome the elite event EE-GH7, wherein said Elite Event is the genetic locus comprising an inserted foreign DNA containing a chimeric HPPD W336 protein-coding gene and a chimeric 2mEPSPS protein-coding gene and 5' and 3' flanking sequences immediately surrounding said inserted foreign DNA, as found in the reference seed deposited in the ATCC with deposit number ML / a / ZUZZ / UU fufó RTA-122856. 19. A transgenic cotton plant, cell, part, tissue, seed or progeny thereof, comprising in its genome event EE-GH7 characterized by a nucleic acid molecule comprising a nucleotide sequence essentially similar to SEQ ID NO: 1 from nucleotide 1207 to nucleotide 1228 and a nucleic acid molecule comprising a nucleotide sequence essentially similar to SEQ ID NO: 1 from nucleotide 8022 to 8043, or the complement of such sequences. 20. A cotton plant, cell, part, tissue, seed or progeny thereof, comprising EE-GH7 and comprising in the genome of its cells a nucleic acid sequence with at least 80%, 90%, 95% or 100% sequence identity with SEQ ID NO: 1 from nucleotide 1197 to nucleotide 1238 and a nucleic acid sequence with at least 80%, 90%, 95% or 100% sequence identity up to SEQ ID NO: 1 from nucleotide 8012 to 8053, or the complement of such sequences. 21. The cotton plant in accordance with any of paragraphs 10 to 12, 14, 15 and 17 to 20, which is tolerant to isoxaflutol or glyphosate. 22. The cotton plant, cell, part, tissue, seed or progeny in accordance with any of paragraphs 10 to 21, which also includes: - Event T304-40, which comprises tolerance to glufosinate and the CrylAb gene as described in document WO2008 / 122406. - The GHB119 event comprising tolerance to glufosinate and the Cry2Ae gene as described in document WO2008 / 151780. - The COT102 event comprising the VIP3A gene as described in document WO2004 039986. 23. The cotton plant cell according to any of paragraphs 10 to 12, 14, 15 and 17 to 22, which is a non-propagable plant cell. 24. A method for producing a cotton plant or seed comprising the Elite Event EEGH7, comprising crossing a plant according to any of paragraphs 10 to 12, 14, 15 and 17 to 22 with another cotton plant, and planting the seed obtained from said crossing. 25. A method for producing a cotton plant tolerant to HPPD-inhibiting herbicides and glyphosate, comprising introducing tolerance to HPPD-inhibiting herbicides and glyphosate into the genome of a cotton plant by crossing a first cotton plant lacking a gene encoding HPPD W336 and lacking a gene encoding 2mEPSPS, with the cotton plant of any of paragraphs 10 to 12, 14, 15 and 17 to 22, and selecting a progeny plant tolerant to HPPD-inhibiting herbicides or glyphosate. 26. The method according to paragraph 25, wherein said progeny tolerant to HPPD-inhibiting herbicides or glyphosate is selected by treating growing plants with HPPD-inhibiting herbicides or glyphosate. 27. A cotton product produced from the cotton plant, cell, part, tissue, seed or progeny of any of paragraphs 10 to 22. 28. The cotton product of paragraph 27, comprising fiber, cotton lint, seeds, seed meal or seed oil. 29. The cotton product of paragraphs 27 or 28, wherein said cotton product comprises a nucleic acid producing a diagnostic or event-specific amplicon for EE-GH7. 30. A method for producing a cotton product, comprising obtaining the cotton plant, cell, part, tissue, seed or progeny of one of paragraphs 10 to 22, and producing said cotton product therefrom. 31. The method of paragraph 30, wherein said cotton product is or comprises cotton fiber, cotton lint, seeds, seed meal or seed oil. 32. The method of paragraph 30 or 31, wherein said cotton product comprises a nucleic acid that produces a diagnostic or event-specific amplicon for EE-GH7. 33. A method for weed control, comprising treating the field in which the cotton seeds of any of paragraphs 10 to 22 have been treated by an HPPD-inhibiting herbicide, before the emergence of the cotton plants, but after the seeds have been sown. 34. A method for weed control, comprising treating the cotton plants of any of paragraphs 10 to 12, 14, 15 and 17 to 22 with an HPPD-inhibiting herbicide after the cotton plants have emerged. 35. A method for protecting emerging cotton plants of any of paragraphs 10 to 12, 14, 15 and 17 to 22 from weed competition, comprising treating the field in which said cotton plants are to be grown with an HPPD-inhibiting herbicide, prior to sowing the plants or seeds, followed by sowing said cotton plants or seeds in said pre-treated field. 36. The method in accordance with any of paragraphs to 35, which further comprises treating cotton plants with glyphosate. 37. The process of any of paragraphs 33 to 36, wherein said HPPD-inhibiting herbicide is isoxaflutol. 38. A method for weed control, comprising treating the cotton plants of any of paragraphs 10 to 12, 14, 15 and 17 to 22 with glyphosate after the cotton plants have emerged. 39. Use of the plant, seed, part, cell or progeny thereof or any of paragraphs 10 to 22, to produce cotton fiber. 40. Use of a cotton plant or seed from any of paragraphs 10 to 11 to cultivate a ML / a / ZUZZ / UU fufó cotton plant tolerant to an HPPD inhibitor herbicide or glyphosate. 41. Use of a cottonseed of any of paragraphs 10 to 22 to obtain a cotton product, wherein said cotton product is or comprises cotton fiber, cotton lint, seed, seed meal or seed oil. 42. A method for identifying the Elite Event EE-GH7 in biological samples, the method comprising detecting a specific region of EE-GH7 with a specific primer pair or probe that specifically recognizes the 5' or 3' flanking region of the foreign DNA comprising herbicide tolerance genes in EE-GH7, and part of the foreign DNA contiguous to said 5' or 3' flanking region. 43. The method of paragraph 42, said method comprising amplifying a DNA fragment of between 50 and 1000 bp from a nucleic acid present in said biological samples by a polymerase chain reaction with at least two primers, wherein a first primer recognizes the 5' flanking region of the foreign DNA comprising herbicide tolerance genes in EE-GH7, said 5' flanking region comprising the nucleotide sequence of SEQ ID NO: 1 from nucleotide 1 to nucleotide 1217 or wherein a first primer recognizes the 3' flanking region of the foreign DNA comprising herbicide tolerance genes in EE-GH7, said 3' flanking region comprising the nucleotide sequence of the complement of SEQ ID NO: 1 from nucleotide 8033 to nucleotide 9328,and wherein a second primer recognizes a sequence within the foreign DNA comprising the nucleotide sequence of SEQ ID NO: 1 from nucleotide 1218 to nucleotide 8032 or its complement. 44. The method of paragraph 43, wherein said first primer recognizing the 5' flanking region comprises a sequence of 17 to 200 consecutive nucleotides selected from the nucleotide sequence of SEQ ID NO: 1 from nucleotide 1 to nucleotide 1217 or said first primer recognizing the 3' flanking region of EE-GH7 comprises a nucleotide sequence of 17 to 200 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID NO: 1 from nucleotide 8033 to nucleotide 9328, and said second primer recognizing a sequence within the foreign DNA comprises 17 to 200 consecutive nucleotides selected from the nucleotide sequence of SEQ ID NO: 1, from nucleotide 1218 to nucleotide 8032 or its complement. 45. The method of paragraph 43, wherein said first primer recognizing the 5' flanking region comprises at its 3' end a sequence of at least 17 consecutive nucleotides selected from the nucleotide sequence of SEQ ID NO: 1, from nucleotide 1 to nucleotide 1217, or said first primer recognizing the 3' flanking region of EE-GH7 comprises at its 3' end a sequence of at least 17 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID NO: 1 from nucleotide 8033 to nucleotide 9328, and said second primer recognizing a sequence within the foreign DNA comprises at its 3' end at least 17 consecutive nucleotides selected from the nucleotide sequence of SEQ ID NO: 1 from nucleotide 1218 to nucleotide 1218. 8032 or its complement. 46. The method of paragraph 45, wherein said primers comprise the sequence of SEQ ID NO: 3 and SEQ ID NO: 4, respectively, or the sequence of SEQ ID NO: 5 and SEQ ID NO: 6, respectively, or the sequence of SEQ ID NO: 11 and SEQ ID NO: 13, respectively. 47. The method of paragraph 46, wherein said primers comprise at their 3' terminal end the sequences SEQ ID NO: 3 and SEQ ID NO: 4, respectively, or comprise at their 3' terminal end the sequence SEQ ID NO: 5 and SEQ ID NO: 6, respectively, or comprise at their 3' terminal end the sequence SEQ ID NO: 11 and SEQ ID NO: 13, respectively. 48. The method of paragraph 46 or 47, wherein said primers consist of the sequence SEQ ID NO: 3 and SEQ ID NO: 4, respectively, or the sequence SEQ ID NO: 5 and SEQ ID NO: 6, respectively, or the sequence SEQ ID NO: 11 and SEQ ID NO: 13, respectively. 49. The method of any of paragraphs 46 to 48, comprising the amplification of a fragment of approximately 126 or 120 bp using the POR EE-GH7 Identification Protocol. 50. The method of any of paragraphs 43 to 49, further comprising the hybridization step of a probe specific to the amplified DNA fragment with at least two primers. 51. The method of paragraph 50, wherein said probe recognizes part of said 5' flanking region and part of the foreign DNA contiguous thereto, or wherein said probe recognizes part of said 3' flanking region and part of the foreign DNA contiguous thereto. 52. The method of paragraph 51, wherein said primers comprise the sequence SEQ ID NO: 5 and SEQ ID NO: 6, respectively, and wherein said probe comprises the sequence SEQ ID NO: 7. 53. A kit comprising a first primer recognizing the 5' flanking region of the foreign DNA comprising herbicide tolerance genes in EE-GH7, said 5' flanking region comprising the nucleotide sequence of SEQ ID NO: 1 from nucleotide 1 to nucleotide 1217 or a first primer recognizing the 3' flanking region of the foreign DNA comprising herbicide tolerance genes in EE-GH7, said 3' flanking region comprising the nucleotide sequence of the complement of SEQ ID NO: 2 from nucleotide 8033 to nucleotide 9328, and a second primer recognizing a sequence within the foreign DNA, said foreign DNA comprising the nucleotide sequence of SEQ ID NO: 1 from nucleotide 1218 to nucleotide 8032 or its complement. 54. The kit of paragraph 53, wherein said first primer recognizing the 5' flanking region comprises a sequence of 17 to 200 consecutive nucleotides selected from the nucleotide sequence of SEQ ID NO: 1 from nucleotide 1 to nucleotide 1217 or said first primer recognizing the 3' flanking region of EE-GH7 comprises a sequence of 17 to 200 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID NO: 1 from nucleotide 8033 to nucleotide 9328, and said second primer recognizes a sequence within the foreign DNA comprising 17 to 200 consecutive nucleotides selected from the nucleotide sequence of SEQ ID NO: 1, from nucleotide 1218 to nucleotide 8032 or ΜΛ / a / ZUZZ / UU l U14 its complement. 55. The kit of paragraph 53, wherein said first primer recognizing the 5' flanking region comprises at its 3' end a sequence of at least 17 consecutive nucleotides selected from the nucleotide sequence of SEQ ID NO: 1 from nucleotide 1 to nucleotide 1217 or said first primer recognizing the 3' flanking region of EE-GH7 comprises at its 3' end a sequence of at least 17 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID NO: 1 from nucleotide 8033 to nucleotide 9328, and said second primer recognizing a sequence within foreign DNA comprises at its 3' end at least 17 consecutive nucleotides selected from the nucleotide sequence of SEQ ID NO: 1 from nucleotide 1218 to nucleotide 8032 or its complement. 56. The kit of paragraph 53, comprising a primer comprising the sequence SEQ ID NO: 3 and a primer comprising the sequence SEQ ID NO: 4 or comprising a primer comprising the sequence SEQ ID NO: 5 and a primer comprising the sequence SEQ ID NO: 6, or comprising a primer comprising the sequence SEQ ID NO: 11 and a primer comprising the sequence SEQ ID NO: 13. 57. The kit of paragraph 53, further comprising a probe that recognizes a sequence between the primer that recognizes the 5' flanking region and the primer that recognizes the sequence within the foreign DNA, or that recognizes a sequence between the primer that recognizes the 3' flanking region and the primer that recognizes the sequence within the foreign DNA. 58. The kit of paragraph 57, wherein said probe recognizes part of said 5' flanking region and part of the foreign DNA contiguous thereto, or wherein said probe recognizes part of said 3' flanking region and part of the foreign DNA contiguous thereto. 59. The kit of paragraph 58, wherein said primers comprise the sequence SEQ ID NO: 5 and SEQ ID NO: 6, and wherein said probe comprises the sequence SEQ ID NO: 7. 60. A primer suitable for use in a specific detection of EE-GH7, comprising a sequence that, under optimized detection conditions, specifically recognizes a sequence within the 5' or 3' flanking region of foreign DNA comprising herbicide tolerance genes in EE-GH7, said 5' flanking region comprising the nucleotide sequence of SEQ ID NO: 1 from nucleotide 1 to nucleotide 1217 and said 3' flanking region comprising the nucleotide sequence of the complement of SEQ ID NO: 1 from nucleotide 8033 to nucleotide 9328. 61. A primer comprising at its 3' terminal end the sequence SEQ ID NO: 3, or the sequence SEQ ID NO: 5, or the sequence SEQ ID NO: 11. 62. A pair of primers comprising a first primer recognizing the 5' flanking region of the foreign DNA comprising herbicide tolerance genes in EE-GH7, said 5' flanking region comprising the nucleotide sequence of SEQ ID NO: 1 from nucleotide 1 to nucleotide 1217 or a first primer recognizing the 3' flanking region of the foreign DNA that ML / a / ZUZZ / UU comprises herbicide tolerance genes in EE-GH7, said 3' flanking region comprising the nucleotide sequence of the complement of SEQ ID NO: 1 from nucleotide 8033 to nucleotide 9328, and a second primer recognizing a sequence within foreign DNA comprising the nucleotide sequence of SEQ ID NO: 1 from nucleotide 1218 to nucleotide 8032 or its complement. 63. A pair of primers according to paragraph 62, wherein said first primer recognizing the 5' flanking region comprises a sequence of 17 to 200 consecutive nucleotides selected from the nucleotide sequence of SEQ ID NO: 1 from nucleotide 1 to nucleotide 1217 or said first primer recognizing the 3' flanking region of EE-GH7 comprises a nucleotide sequence of 17 to 200 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID NO: 1 from nucleotide 8033 to nucleotide 9328, and said second primer recognizing a sequence within the foreign DNA comprises 17 to 200 consecutive nucleotides selected from the nucleotide sequence of SEQ ID NO: 1 from nucleotide 1218 to nucleotide 8032 or its complement. 64. A pair of primers according to paragraph 62, wherein said first primer recognizing the 5' flanking region comprises at its 3' end a sequence of at least 17 consecutive nucleotides selected from the nucleotide sequence of SEQ ID NO: 1 from nucleotide 1 to nucleotide 1217 or said first primer recognizing the 3' flanking region of EE-GH7 comprises at its 3' end a nucleotide sequence of at least 17 consecutive nucleotides selected from the nucleotide sequence of the complement of SEQ ID NO: 1 from nucleotide 8033 to nucleotide 9328, and said second primer recognizing a sequence within the foreign DNA comprises at its 3' end at least 17 consecutive nucleotides selected from the nucleotide sequence of SEQ ID NO: 1 from nucleotide 1218 to nucleotide 8032 or its complement. 65. A pair of primers, comprising a first primer comprising the sequence SEQ ID NO: 3 and a second primer comprising the sequence SEQ ID NO: 4, or comprising a first primer comprising the sequence SEQ ID NO: 5 and a second primer comprising the sequence SEQ ID NO: 6, or comprising a first primer comprising the sequence SEQ ID NO: 11 and a second primer comprising the sequence SEQ ID NO: 13. 66. A pair of primers, comprising a first primer comprising at its 3' end the sequence SEQ ID NO: 3 and a second primer comprising at its 3' end the sequence SEQ ID NO: 4, or comprising a first primer comprising at its 3' end the sequence SEQ ID NO: 5, and a second primer comprising at its 3' end the sequence SEQ ID NO: 6, or comprising a first primer comprising at its 3' end the sequence SEQ ID NO: 11 and a second primer comprising at its 3' end the sequence SEQ ID NO: 13. 67. A pair of primers, comprising a first primer consisting of the sequence SEQ ID NO: 3 and a second primer consisting of the sequence SEQ ID NO: 4, or comprising a first primer consisting of the sequence SEQ ID NO: 5 and a second primer consisting of the sequence ML / a / ZUZZ / UU fufó SEQ ID NO: 6, or comprising a first primer consisting of the sequence SEQ ID NO: 11 and a second primer consisting of the sequence SEQ ID NO: 13. 68. The method in paragraph 42, comprising hybridization of a nucleic acid from biological samples with a probe specific for EE-GH7. 69. The method of paragraph 68, wherein the sequence of said specific probe has at least 80% sequence identity with a sequence comprising part of the 5' flanking sequence or the 3' flanking sequence of EE-GH7 and the sequence of foreign DNA contiguous thereto. 70. The method of paragraph 69, wherein the sequence of said specific probe has at least 80% sequence identity with SEQ ID NO: 1 from nucleotide 1207 to 1228 or SEQ ID NO: 1 from nucleotide 8022 to 8043, or the complement of said sequences. 71. The method of paragraph 69, wherein the sequence of said specific probe has at least 80% sequence identity with SEQ ID NO: 1 from nucleotide 1197 to 1238 or SEQ ID NO: 1 from nucleotide 8012 to 8053, or the complement of said sequences. 72. The method of paragraph 71, wherein said probe comprises the sequence SEQ ID NO: 7. 73. A kit for identifying the Elite Event EE-GH7 in biological samples, said kit comprising a specific probe, capable of specifically hybridizing with a specific region of EE-GH7. 74. The kit of paragraph 73, wherein the sequence of said specific probe has at least 80% sequence identity with a sequence comprising part of the 5' flanking sequence or the 3' flanking sequence of the foreign DNA comprising herbicide tolerance genes in EE-GH7 and the sequence of the foreign DNA contiguous thereto. 75. The kit of paragraph 74, wherein the sequence of said specific probe comprises a nucleotide sequence having at least 80% sequence identity with SEQ ID NO: 1 from nucleotide 1197 to 1238 or SEQ ID NO: 1 from nucleotide 8012 to 8053, or the complement of said sequences. 76. A specific probe for the identification of the Elite Event EE-GH7 in biological samples. 77. The probe of paragraph 76, comprising a nucleotide sequence having at least 80% sequence identity with a sequence comprising part of the 5' flanking sequence or the 3' flanking sequence of the foreign DNA comprising herbicide tolerance genes in EE-GH7 and the sequence of foreign DNA contiguous thereto, or the complement thereof. 78. The probe of paragraph 77 having at least 80% sequence identity with SEQ ID NO: 1 from nucleotide 1207 to 1228 or SEQ ID NO: 1 from nucleotide 8022 to 8043, or the complement of such sequences. 79. A specific probe comprising a nucleotide sequence that is essentially similar to SEQ ID NO: 1 from nucleotide 1197 to 1238 or SEQ ID NO: 1 from nucleotide 8012 to 8053, or the complement of such sequences. 80. A specific probe consisting of the nucleotide sequence of SEQ ID NO: 1 from nucleotide 1197 to 1238 or SEQ ID NO: 1 from nucleotide 8012 to 8053, or the complement of such ML / a / ZUZZ / UU fufed sequences. 81. A specific probe comprising the sequence SEQ ID NO: 7. 82. The primer or primer pair or probe according to any of paragraphs 60 to 67 and 76 to 81, comprising a nucleotide sequence not associated with the 5' end, or that is labeled. 83. A method for verifying seed purity, comprising detecting a specific EE-GH7 region with a specific primer or probe that specifically recognizes the 5' or 3' flanking region of foreign DNA comprising herbicide tolerance genes in EE-GH7, in seed samples. 84. The method of paragraph 83, comprising amplifying a DNA fragment of between 50 and 1000 bp from nucleic acid present in said biological samples using a polymerase chain reaction with at least two primers, one of which recognizes the 5' flanking region of the foreign DNA comprising herbicide tolerance genes in EE-GH7, said 5' flanking region comprising the nucleotide sequence of SEQ ID NO: 1 from nucleotide 1 to nucleotide 1217 or the 3' flanking region of the foreign DNA comprising herbicide tolerance genes in EE-GH7, said 3' flanking region comprising the nucleotide sequence of the complement of SEQ ID NO: 1 from nucleotide 8033 to nucleotide 9328, the other primer of said primers recognizing a sequence within the foreign DNA comprising the nucleotide sequence of SEQ ID NO: 1 from nucleotide 1218 through nucleotide 8032 or its complement,and performs the hybridization of a probe specific to the amplified DNA fragment with at least two primers. 85. The method of paragraph 84, comprising amplifying a 120 bp DNA fragment and wherein said primers comprise the sequences SEQ ID NO: 5 and SEQ ID NO: 6, respectively, and wherein said probe comprises the sequence SEQ ID NO: 7. 86. A method for assessing the presence of EE-GH7 in seeds, comprising detecting a specific region of EE-GH7 with a primer or probe that specifically recognizes the 5' or 3' flanking region of foreign DNA comprising herbicide tolerance genes in EE-GH7, in seed lot samples. 87. The method of paragraph 86, comprising amplifying a DNA fragment of between 50 and 1000 bp from nucleic acid present in said biological samples using a polymerase chain reaction with at least two primers, one of which recognizes the 5' flanking region of the foreign DNA comprising herbicide tolerance genes in EE-GH7, said 5' flanking region comprising the nucleotide sequence of SEQ ID NO: 1 from nucleotide 1 to nucleotide 1217 or the 3' flanking region of the DNA comprising herbicide tolerance genes in EE-GH7, said 3' flanking region comprising the nucleotide sequence of the complement of SEQ ID NO: 1 from nucleotide 8033 to nucleotide 9328, the other primer of said primer pair comprising a sequence within the foreign DNA having the nucleotide sequence of SEQ ID ινΐΛ / a / zuzz / uu fufó NO: 1 from nucleotide 1218 to nucleotide 8032 or its complement, and hybridize into a probe specific for the amplified DNA fragment with at least two primers. 88. The method of paragraph 87, comprising amplifying a 120 bp DNA fragment and wherein said primers comprise the sequences SEQ ID NO: 5 and SEQ ID NO: 6, respectively, and wherein said probe comprises the sequence SEQ ID NO: 7. 89. A method for determining the zygosity status of a plant, plant material, or seed comprising the Elite Event EE-GH7, said method comprising amplified DNA fragments of between 50 and 1000 bp from a nucleic acid present in said biological samples using a polymerase chain reaction with at least three primers, two of said primers specifically recognizing plant DNA prior to insertion, such as a primer comprising the nucleotide sequence of SEQ ID NO: 11 and a primer comprising the nucleotide sequence of SEQ ID NO: 12, the third of said primers recognizing a sequence within foreign DNA, such as the nucleotide sequence of SEQ ID NO: 13. 90. A method for detecting the presence of the Elite Event EE-GH7 in biological samples by hybridization with a substantially labeled complementary nucleic acid probe wherein the probe:target nucleic acid ratio is amplified through recycling of the target nucleic acid sequence, said method comprising: a) Hybridization of said target nucleic acid sequence to a first nucleic acid oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 1 from nucleotide 1218 to nucleotide 1235 or its complement or said first nucleic acid oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 1 nucleotide 8015 to 8032 or its complement. b) Hybridization of said target nucleic acid sequence with a second nucleic acid oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 1 from nucleotide 1200 to nucleotide 1217 or its complement or said labeled nucleic acid probe comprising the nucleotide sequence of SEQ ID NO: 1 from nucleotide 8033 to nucleotide 8050 or its complement, wherein said first and second oligonucleotide overlap by at least one nucleotide and wherein said first or second oligonucleotide is labeled to be said labeled nucleic acid probe. c) Excise only the labeled probe within the probe: target nucleic acid sequence with an enzyme that causes selective cleavage of the probe resulting in duplex dissociation, leaving the target sequence intact. d) Recycle the target nucleic acid sequence by repeating steps (a) to (c). e) Detect the cleaved labeled probe, thus determining the presence of said target nucleic acid sequence. The following examples, which are not intended to limit the invention to the specific embodiments described, can be understood in conjunction with the accompanying Figures, incorporated herein by reference. ML / a / ZUZZ / UU fufó BRIEF DESCRIPTION OF THE FIGURES Figure 1. Schematic representation of the relationship between the cited nucleotide sequences and the primers. Black bar: foreign DNA; dashed bar: plant-derived DNA; gray bar: target site removal. Black arrows: oligonucleotide primers; black line: oligonucleotide probe. The numbers above or below the bars representing SEQ ID No. 2 and 1 represent the nucleotide positions of the different genetic elements in those sequences. The numbers above the primer combinations indicate the length of the fragment produced in a polymerase chain reaction with these primers. The tables next to the primers represent the nucleotide positions of the primers in SEQ ID No. 2 or SEQ ID No. 1. Note: The diagram is not drawn to scale. Figure 2. Results obtained by the PCR Identification Protocol developed for EEGH7. Gel loading sequence: Lane 1: molecular weight marker (50 bp); lanes 2, 3, and 4: negative control (no template); lanes 5, 6, and 7: DNA from wild cotton plants; lanes 8, 9, and 10: DNA samples from cotton plants comprising the EE-GH7 transgenic event; lanes 11, 12, and 13: negative control (no template); lane 14: molecular weight marker (50 bp). The numbers indicate the size of the marker fragments and the specific EE-GH7 fragment. Figure 3. Results of the real-time PCR assay for the detection of EE-GH7 in bulk seeds. The graph shows the relative fluorescence on a logarithmic scale for 1:5 dilutions of DNA comprising EE-GH7. The X-axis represents the PCR cycle. Horizontal bar: threshold level, which for this experiment is 0.135494. Figure 4. TaqMan endpoint assay results for the detection of EE-GH7. Y-axis: signal-to-background noise ratio (S / B); X-axis: sample number. White bars marked “a”: Target (EE-GH7); gray bars marked “b”: endogenous control; horizontal line marked “1”: lower threshold level for the target (EE-GH7); horizontal line marked “2”: threshold for the endogenous control; horizontal line marked “3”: upper-level negative control. Samples: A1–A8: samples comprising EE-GH7; A9 and A10: wild-type cotton plant samples not comprising EE-GH7; A11 and A12: negative control (no mold). Figure 5. Results obtained by the zlgosis scoring PCR protocol developed for EE-GH7. Y-axis: Background signal (S / B) VIC; X-axis: Background signal (S / B) FAM. Black dots: samples. Lines “a” delineate wild-type samples; lines “b” delineate hemizygous samples for EE-GH7; lines “c” delineate homozygous samples for EE-GH7. “d”: minimum S / B VIC; e: minimum S / B FAM. DETAILED DESCRIPTION OF THE INVENTION In this invention, EE-GH7 has been identified as an Elite Event of a population of transgenic cotton plants in the development of a herbicide-tolerant cotton (Gossypium hirsutum) comprising a gene conferring tolerance to glyphosate combined with a gene conferring tolerance to 4-hydroxyphenylpyruvate dioxygenase (HPPD) inhibitors, each under the control of a plant-expressible promoter. The incorporation of a recombinant DNA molecule into the plant genome generally results from the transformation of a cell or tissue. The specific site of incorporation is usually subject to random integration. The DNA introduced into the plant genome as a result of transforming a plant cell or tissue with recombinant or transforming DNA, and originating from said transforming DNA, hereinafter referred to as foreign DNA, comprises one or more transgenes. The transgenes in EE-GH7 are the glyphosate herbicide tolerance and HPPD inhibitor genes. Plant DNA in the context of the present invention refers to the DNA originating from the transformed plant. The plant DNA will generally be found at the same genetic locus in the corresponding wild-type plant. The foreign DNA can be characterized by the location and configuration at the insertion site of the recombinant DNA molecule in the plant genome. The site in the plant genome where the recombinant DNA is inserted is also known as the insertion site or target site.The insertion of recombinant DNA into the region of the plant genome called pre-insertion plant DNA may be associated with the deletion of plant DNA, called target site deletion. A flanking region or flanking sequence, as used herein, refers to a sequence of at least 20 bp, preferably at least 50 bp, and up to 5000 bp of DNA other than the introduced DNA, preferably plant genome DNA, that is located immediately upstream and contiguous with, or immediately downstream and contiguous with, the foreign DNA. Transformation procedures that lead to the random integration of the foreign DNA will result in transformants with different flanking regions, which are characteristic and unique to each transformant.When recombinant DNA is introduced into a plant through traditional crossing, its insertion site in the plant genome or its flanking regions will generally not be modified. An isolated nucleic acid (sequence or molecule) or isolated DNA (sequence or molecule), as used herein, refers to a nucleic acid or DNA (sequence or molecule) that is no longer found in the natural environment from which it was isolated, e.g., the nucleic acid sequence in another bacterial host or in the genome of a plant, or a nucleic acid or DNA fused to DNA or a nucleic acid of another origin, such as when contained in a chimeric gene under the control of a promoter expressible in plants.Any nucleic acid or DNA of this invention, including any primer, may also occur non-naturally, such as a nucleic acid or DNA with a sequence identical to a sequence occurring in nature but having a tag (absent in its naturally occurring counterpart), or with a sequence having at least one addition or replacement of nucleotides or at least one internal deletion of nucleotides compared to a naturally existing nucleotide, or with a sequence having less than 100% sequence identity (not identical) to a naturally existing nucleic acid or DNA or a fragment thereof, or a nucleic acid or DNA with a sequence consisting of nucleotide sequences of different types. ML / a / ZUZZ / UU fufó origins that do not occur together in nature (a chimeric or hybrid DNA), or a nucleic acid or synthetic DNA with a sequence different from that of a natural nucleic acid or DNA, or a fragment thereof. An event is defined as a (artificial) genetic locus that, as a result of genetic engineering, carries foreign DNA or a transgene comprising at least one copy of a gene or genes of interest. The typical allelic states of an event are the presence or absence of the foreign DNA. An event is phenotypically characterized by the expression of the transgene. At the genetic level, an event is part of a plant's genetic makeup. At the molecular level, an event can be characterized by the restriction map (e.g., as determined by Southern blotting), by the upstream or downstream flanking sequences of the transgene, the location of molecular markers, or the molecular configuration of the transgene. Typically, the transformation of a plant with transformant DNA comprising at least one gene of interest leads to a population of transformants comprising a multitude of separate events, each of which is unique.An event is characterized by foreign DNA and at least one flanking sequence. An Elite Event, as used herein, is an event selected from a group of events, obtained by transformation with the same transforming DNA, based on optimal trait efficacy and superior expression, the stability of the transgene(s), and its compatibility with optimal agronomic characteristics of the plant it comprises. Therefore, the criteria for the selection of elite events are one or more, preferably two or more, advantageously all of the following: a) Trait effectiveness. b) That the presence of foreign DNA does not compromise other desired characteristics of the plant, such as those related to agronomic performance or commercial value. c) That the event is characterized by a well-defined molecular structure that is stably inherited and for which appropriate identity control tools can be developed. d) That the genes of interest show a correct, appropriate and stable spatial and temporal phenotypic expression, at a commercially acceptable level in a range of environmental conditions in which the plants carrying the event are likely to be exposed to normal agronomic use. It is preferred that the foreign DNA be associated with a position in the plant genome that allows easy introgression into the desired commercial genetic backgrounds. The status of an event as an Elite Event is confirmed by the introgression of the Elite Event into different relevant genetic backgrounds and observing compliance with one, two, three or all of the above criteria, e.g. a), b), c) and d). Therefore, an Elite Event refers to a genetic locus comprising foreign DNA that meets the criteria described above. A plant, plant material, or progeny, such as seeds, may contain one or more Elite Events in its genome. The tools developed to identify an Elite Event or the plant or plant material comprising an Elite Event, or products comprising plant material comprising the Event ML / a / ZUZZ / UU fufó Elite, are based on the specific genomic characteristics of the Elite Event, such as a specific restriction map of the genomic region comprising the foreign DNA, molecular markers, or the sequence of the region or regions flanking the foreign DNA. Once one or both flanking regions of the foreign DNA have been sequenced, primers and probes that specifically recognize this sequence(s) in the nucleic acid (DNA or RNA) of a sample can be developed using a molecular biology technique. For example, a PCR method can be developed to identify the Elite Event in biological samples (such as plant samples, plant material, or products comprising plant material). Such a PCR relies on at least two specific primers: one that recognizes a sequence within the 5' or 3' flanking region of the Elite Event and the other that recognizes a sequence within the foreign DNA.The primers preferably have a sequence of between 15 and 35 nucleotides that, under optimized PCR conditions, specifically recognize a sequence within the 5' or 3' flanking region of the Elite Event and the foreign DNA of the Elite Event, respectively, so that the fragment (integration fragment or discriminant amplicon) is amplified from a nucleic acid sample comprising the Elite Event. This means that only the targeted integration fragment, and no other sequence in the plant genome or the foreign DNA, is amplified under optimized PCR conditions. Suitable PCR primers for the invention may be the following: - Oligonucleotides ranging in length from 17 nt to approximately 200 nt, comprising a nucleotide sequence of at least 17 consecutive nucleotides, preferably 20 consecutive nucleotides, selected from plant DNA in the flanking 5' sequence (SEQ ID NO: 1 from nucleotide 1 to nucleotide 1217) at its end 3'(primers that recognize flanking sequences 5'). - Oligonucleotides with a length of 17 nt to approximately 200 nt, comprising a nucleotide sequence of at least 17 consecutive nucleotides, preferably 20 consecutive nucleotides, selected from plant DNA in the 3' flanking sequence (complement to SEQ ID NO: 1 from nucleotide 8033 to nucleotide 9328) at its 3' end (3' flanking primers recognizing the flanking sequences). - Oligonucleotides ranging in length from 17 nt to approximately 200 nt, comprising a nucleotide sequence of at least 17 consecutive nucleotides, preferably 20 consecutive nucleotides, selected from the inserted DNA sequences (complement to SEQ ID NO: 1 from nucleotide 1218 to nucleotide 8032) at their 3' end (foreign DNA recognizing primers). - Oligonucleotides ranging in length from 17 nt to approximately 200 nt, comprising a nucleotide sequence of at least 17 consecutive nucleotides, preferably 20 consecutive nucleotides, selected from the inserted DNA sequences (SEQ ID NO: 1 from nucleotide 1218 to nucleotide 8032) at their 3' end (foreign DNA recognizing primers). - Suitable oligonucleotides with a length of 17 nt to approximately 200 nt, which ML / a / ZUZZ / UU fufó comprise a nucleotide sequence of at least 17 consecutive nucleotides, preferably 20 consecutive nucleotides, selected from the nucleotide sequence of the inserted DNA fragment or its complement (SEQ ID NO: 1 from nucleotide 1218 to nucleotide 8032). It will be understood that primers recognizing 5' flanking sequences can be used in a POR reaction together with primers recognizing foreign DNA selected from the complement of SEQ ID NO: 1 from nucleotide 1218 to nucleotide 8032, whereas primers recognizing 3' flanking sequences can be used in a POR reaction together with primers recognizing foreign DNA selected and directed to SEQ ID NO: 1 from nucleotide 1218 to nucleotide 8032. Primers can be longer than the 17 consecutive nucleotides mentioned, and may be, for example, 20, 21, 30, 35, 50, 75, 100, 150, 200 nucleotides long, or even longer. Primers may consist entirely of a nucleotide sequence selected from the aforementioned nucleotide sequences of flanking sequences and foreign DNA. However, the nucleotide sequence of the primers at their 5' end (i.e., outside the 17 consecutive nucleotides located at 3j) is less critical. Therefore, the 5' sequence of the primers may comprise or consist of a nucleotide sequence selected from the flanking sequences or foreign DNA, as appropriate, but may contain several (1, 2, 5, or 10) mismatches.The 5' sequence of the primers can even be entirely a nucleotide sequence unrelated to the flanking sequences or foreign DNA, such as, for example, a nucleotide sequence representing one or more restriction enzyme recognition sites, such as nucleotide sequences capable of binding to other oligonucleotides, or such as labeled oligonucleotides, for example, FRET cassettes (LGC genomics; see Semagn et al., 2014, Mol Breeding 33:1-14, and US 7615620). Such unrelated sequences or mismatched flanking DNA sequences should preferably not exceed 100, 50, or even 25 nucleotides. The primers can also be modified with a marker, such as a fluorescent probe. In addition, suitable primers may comprise or consist (essentially) of a nucleotide sequence at its 3' end spanning the junction region between the plant DNA-derived sequences and the foreign DNA sequences (located at nucleotides 1217 and 1218 in SEQ ID NO: 1 and nucleotides 8032 and 8033 in SEQ ID NO: 1) provided that the aforementioned 17 consecutive nucleotides located at 3' do not derive exclusively from foreign DNA or plant-derived sequences in SEQ ID NO: 1. It will also be immediately clear to the subject expert that properly selected POR primer pairs should not comprise complementary sequences to each other. The primers and probes according to the invention can be marked, such as, for example, with fluorescent markers or silencers as described in another section of this document. According to the invention, the primers may have unrelated sequences at the 5' end. According to the invention, the probes may have unrelated sequences at the 5' or 3' end. Such unrelated sequences may, for example, be sequences designed to bind to secondary primers, or they may be sequences comprising restriction sites, or any other unrelated sequence. For the purposes of the invention, the complement of a nucleotide sequence represented in SEQ ID No: X is the nucleotide sequence that can be derived from the represented nucleotide sequence by replacing the nucleotides with their complementary nucleotides according to Chargaff's rules (A+>T; G<=>C) and reading the sequence in the 5' to 3' direction, i.e., in the opposite direction to the represented nucleotide sequence. Some examples of suitable primers are the oligonucleotide sequences of SEQ ID NO: 3, SEQ ID NO: 5 or SEQ ID NO: 11 (5' flanking sequence recognition primer), or SEQ ID NO: 4, SEQ ID NO: 6 (foreign DNA recognition primer for use with the 5' flanking sequence recognition primers). Preferably, the amplified fragment is between 50 and 500 nucleotides long, or between 50 and 150 nucleotides long. The specific primers may have a sequence that is 80% to 100% identical to a sequence within the 5' or 3' flanking region of the Elite Event and the Elite Event's foreign DNA, respectively, provided that the mismatches still allow for the specific identification of the Elite Event with these primers under optimized PCR conditions. However, the range of permissible mismatches can be easily determined experimentally and is known to experts in the technique. The detection of integration fragments can be carried out in several ways, for example, through size estimation after gel analysis. Integration fragments can also be sequenced directly. Other sequencing methods specific to the detection of amplified DNA fragments are also known in the field. Amplified DNA fragments can also be detected using labeled sequences and molecular tags. For example, a labeled probe that binds specifically to the amplified fragment can be included in the reaction mixture. The labeled probe (FRET hybridization probe) can comprise a fluorescent marker and a switch, so that the FRET cassette is no longer silenced and fluoresces when it binds to the PCR product.Alternatively, a labeled FRET cassette—that is, an oligonucleotide labeled with a fluorescent tag and a silencer—can be included in the reaction mixture. This cassette specifically binds to one of the primers in the reaction mixture, such as a FRET cassette targeting a 5' extension of the primer used in the reaction mixture (see, for example, Semagn et al., 2014, Mol Breeding 33: 1–14, and US 7615620). Fluorescence can be quantified using methods known to the technique. Fluorescence can be measured in real time, i.e., during each cycle of the PCR reaction. Fluorescence can also be measured at the end of the PCR reaction. Because the primer sequence and its relative location in the genome are unique to the Elite Event, amplification of the integration fragment will occur only in biological samples that ML / a / ZUZZ / UU comprises (the nucleic acid of) the Elite Event. Preferably, when performing PCR to identify the presence of EE-GH7 in unknown samples, a control of a set of primers is included with which a fragment within a constitutive gene of the event plant species can be amplified. Constitutive genes are genes that are expressed in most cell types and are related to basic metabolic activities common to all cells. Preferably, the amplified fragment of the constitutive gene is a fragment that is larger than the amplified integration fragment. Depending on the samples being tested, other controls may be included. Standard PCR protocols are described in the technique, such as in the PCR Applications Manual (Roche Molecular Biochemicals, 2nd edition, 1999) and other references. Optimal PCR conditions, including the specific primer sequence, are specified in a PCR (or Polymerase Chain Reaction) Identification Protocol for each Elite Event. However, it is understood that several parameters in the PCR Identification Protocol may need to be adjusted to specific laboratory conditions and can be slightly modified to achieve similar results. For example, using a different method for DNA preparation may require adjusting, for instance, the number of primers, the polymerase, and the hybridization conditions used. Similarly, selecting different primers may dictate different optimal conditions for the PCR Identification Protocol.However, the adjustments will be obvious to someone skilled in the technique, and are also detailed in current PCR application manuals, such as the one cited earlier in this document. Alternatively, specific primers can be used to amplify an integration fragment that can be used as a specific probe to identify EE-GH7 in biological samples. Contacting the nucleic acid of a biological sample with the probe, under conditions that allow hybridization of the probe with its corresponding fragment in the nucleic acid, results in the formation of a nucleic acid / probe hybrid. The formation of this hybrid can be detected (e.g., through titration of the nucleic acid or the probe), and therefore, the formation of this hybrid indicates the presence of EE-GH7. Such identification methods based on hybridization with a specific probe (either on a solid-phase support or in solution) have been described in the art. The specific probe is preferably a sequence that, under optimized conditions, hybridizes specifically to a region within the 5' or 3' flanking region of the Elite Event and preferably also comprises part of the foreign DNA contiguous to it (hereafter, the specific region). Preferably, the specific probe comprises a sequence of between 50 and 500 bp, preferably 100 to 350 bp, that is at least 80%, preferably between 80 and 85%, more preferably between 85 and 90%, especially preferably between 90 and 95%, and most preferably between 95% and 100% identical to (or complementary to) the nucleotide sequence of a specific region.Preferably, the specific probe will comprise a sequence of approximately 15 to approximately 100 contiguous nucleotides identical (or complementary) to a specific region of the Elite Event. Suitable oligonucleotides used as PCR primers for detecting the EEGH7 Elite Event can also be used to develop a PCR-based protocol for determining the zygosity status of plants containing the Elite Event. For this purpose, two primers that recognize the wild-type locus before integration are designed to face each other, with the insertion site located between them. These primers can specifically recognize the 5' and 3' flanking sequences contained in SEQ ID NO: 1. This set of primers, along with a third complementary primer for transforming DNA sequences (foreign DNA), allows for simultaneous diagnosis by PCR amplification of both the EEGH7-specific locus and the wild-type locus.If the plant is homozygous for the transgenic locus or the corresponding wild-type locus, diagnostic PCR will yield a single typical PCR product, preferably of typical length, for either the transgenic or wild-type locus. If the plant is hemizygous for the transgenic locus, two locus-specific PCR products will appear, reflecting amplification of both the transgenic and wild-type locus. Alternatively, to determine the zygosity status of plants containing the Elite Event, two primers that recognize the wild-type locus are designed prior to integration in such a way that they target each other, and that one primer specifically recognizes the 5' or 3' flanking sequences contained in SEQ ID NO: 1, and that said first primer specifically recognizes the 3' or 5' flanking sequences contained in SEQ ID NO: 1, or specifically recognizes the deletion of the target site.For the present invention, the particularly suitable primers that recognize the wild-type locus prior to integration are primers comprising or consisting (essentially) of the nucleotide sequences of SEQ ID NO: 11 and SEQ ID NO: 12. This set of primers, together with a third primer complementary to transforming DNA sequences (foreign DNA), or complementary to transforming DNA sequences and the 5' or 3' flanking sequences adjacent thereto, and in a direction towards the primer that specifically recognizes the 5' or 3' flanking sequences (such as the primer comprising or consisting (essentially) of the nucleotide sequence of SEQ ID NO: 13, which is in a direction towards the primer comprising or consisting (essentially) of the nucleotide sequence of SEQ ID NO: 11), enables simultaneous PCR amplification of the diagnostic EE-GH7 specific locus, as well as of the wild-type locus.If the plant is homozygous for the transgenic locus or the corresponding wild-type locus, diagnostic PCR will yield a single PCR product typical of either the transgenic or wild-type locus. If the plant is hemizygous for the transgenic locus, two locus-specific PCR products will appear, reflecting amplification of both the transgenic and wild-type locus. The detection of typical PCR products for wild-type and transgenic loci can be based on determining the length of PCR products that may be typical for the locus. ML / a / ZUZZ / UU fufó wild-type and transgenic. Alternatively, detection of typical PCR products for the transgenic and wild-type locus can be performed by modifying the primer specific for target site deletion and the primer specific for foreign DNA, and detecting the incorporation of the modified primers into a PCR product. For example, the primer specific for target site deletion and the primer specific for foreign DNA can be labeled using a fluorescent tag, where the tags are different for the two primers. Fluorescence can be detected when the primer is incorporated into a PCR product. If the plant is homozygous for the transgenic locus or the corresponding wild-type locus, fluorescence of the marker can be detected for the primer specific only for foreign DNA or for the primer specific for target site deletion only.If the plant is hemizygous for the transgenic locus, fluorescence can be detected on both the primer marker specific for foreign DNA and the primer marker specific for target site deletion, reflecting amplification of both the transgenic and natural-type locus. Alternatively, the primer specific for target site removal and the primer specific for foreign DNA may have a 5' extension that binds specifically to a labeled FRET cassette, i.e., an oligonucleotide labeled with a fluorescent tag and an extinguisher, wherein the 5' extension and the corresponding FRET cassettes are different for the two primers (see, for example, Semagn et al., 2014, Mol Breeding 33: 1-14, and US 7615620). Fluorescence can be detected when the primer is incorporated into a PCR product and subsequently when the FRET cassette is incorporated into the PCR product.If the plant is homozygous for the transgenic locus or the corresponding wild-type locus, fluorescence can be detected from the FRET cassette that binds specifically to the primer specific for foreign DNA only or from the FRET cassette that binds specifically to the primer specific for target site deletion only. If the plant is hemizygous for the transgenic locus, fluorescence can be detected from both FRET cassettes that bind specifically to the primer specific for foreign DNA and from the FRET cassette that binds specifically to the primer specific for target site deletion, reflecting both transgenic and wild-type locus amplification. If the plant is homozygous for the transgenic locus or the corresponding wild-type locus, diagnostic PCR will yield a single typical PCR product, preferably of typical length, for either the transgenic or wild-type locus. If the plant is hemizygous for the transgenic locus, two locus-specific PCR products will appear, reflecting amplification of both the transgenic and wild-type locus. Alternatively, to determine the zygosity status of plants containing the Elite Event, the presence of the event can be quantitatively determined by PCR as described in Example 2.2.2. For this purpose, two primers that recognize the transgenic are designed to be directed towards each other, where one primer specifically recognizes the 5' or 3' flanking sequence contained within SEQ ID NO: 1, (as a primer ML / a / ZUZZ / UU l U14 comprising or consisting (essentially) of the nucleotide sequence of SEQ ID NO: 5) and wherein a primer specifically recognizes foreign DNA within SEQ ID NO: 1 (such as a primer comprising or consisting (essentially) of the nucleotide sequence of SEQ ID NO: 6). This primer set enables PCR amplification of the specific EE-GH7 locus. The amplified DNA fragment can be quantitatively detected using a labeled probe included in the reaction mixture that binds specifically to the amplified fragment (such as a probe comprising or consisting (essentially) of the nucleotide sequence of SEQ ID NO: 7). The labeled probe (FRET hybridization probe) may comprise a fluorescent tag and a switch, such that the FRET cassette is no longer silenced and fluoresces when bound to the PCR product.Fluorescence can be quantified in real time, i.e., during each cycle of the PCR reaction, using methods known to the technique. The PCR cycle in which fluorescence exceeds a certain threshold level is a measure of the amount of the specific EE-GH7 locus in the biological sample being analyzed, and the zygosity status can be calculated based on homozygous and heterozygous reference samples. Alternatively, the zygosity status of plants containing EE-GH7 can also be determined based on copy number analysis, using TaqMan chemistry and real-time PCR principles. This alternative method will typically include an EE-GH7-specific reaction to quantify the EE-GH7 copy number and a gene-specific endogenous reaction to normalize the EE-GH7 copy number. Samples containing the EE-GH7 event in a homozygous state will have a relative copy number that is twice as high as that of hemizygous samples. Azygous samples will not amplify the EE-GH7 sequence using this method. Furthermore, detection methods specific to the Elite Event EE-GH7, which differ from PCR-based amplification methods, can also be developed using the Elite Event-specific sequence information provided herein. Such alternative detection methods include linear signal amplification detection methods based on invasive cleavage of particular nucleic acid structures, also known as Invader™ technology (as described, for example, in U.S. Patent 5,985,557 Invasive Excision of Nucleic Acids, 6,001,567 “Invader-Directed Excision,” incorporated herein for reference).For this purpose, the target sequence is hybridized with a first labeled nucleic acid oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 1 from nucleotide 1218 to nucleotide 1235 or its complement or said labeled nucleic acid probe, comprising the nucleotide sequence of SEQ ID NO: 1 from nucleotide 8015 to 8032 or its complement and is further hybridized with a second nucleic acid oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 1 from nucleotide 1200 to nucleotide 1217 or its complement or said labeled nucleic acid probe comprising the nucleotide sequence of SEQ ID NO: 1 from nucleotide 8033 to nucleotide 8050 or its complement, wherein the first and second oligonucleotide overlap by at least one nucleotide. The double or triple structure produced by this hybridization allows selective cleavage of the probe with an enzyme (Cleavase®) leaving the target sequence intact.The cleaved labeled probe is subsequently detected, potentially through an intermediate step that results in further signal amplification. A kit, as used herein, refers to a set of reagents for the purpose of performing the method of the invention, particularly the identification of the Elite Event EE-GH7 in biological samples or the determination of the zygosity status of EE-GH7 containing plant material. In particular, a preferred embodiment of the kit of the invention comprises at least one or two specific primers, as described above for the identification of the Elite Event, or three specific primers, or two specific primers and one specific probe, as described above for the determination of the zygosity status. Optionally, the kit may further comprise any other reagents described herein in the PCR Identification Protocol or any of the other protocols described herein for the detection of EE-GH7.Alternatively, according to another embodiment of this invention, the kit may comprise a specific probe, as described above, that specifically hybridizes with nucleic acid from biological samples to identify the presence of EEGH7 therein. Optionally, the kit may further comprise any other reagent (such as, for example, a hybridization buffer or a label) for the identification of EEGH7 in biological samples using the specific probe. The kit of the invention can be used, and its components can be specifically adjusted, for quality control purposes (e.g., purity of seed batches), detection of the presence or absence of the Elite Event in plant material or material comprising or derived from plant material, such as, for example, food products or feed. As used herein, sequence identity with respect to nucleotide sequences (DNA or RNA) refers to the number of positions with identical nucleotides divided by the number of nucleotides in the shorter of the two sequences. Alignment of the two nucleotide sequences is performed using the Wilbur and Lipmann algorithm (Wilbur and Lipmann, 1983, Proc. Nat. Acad. Sci. USA 80: 726) with a window size of 20 nucleotides, a word length of 4 nucleotides, and a gap penalty of 4. Computer-assisted analysis and interpretation of the sequence data, including sequence alignment as described above, can be conveniently performed, for example, using the Genetics Computer Group (GCG, Biotechnology Center, University of Wisconsin) sequence analysis software package.Sequences are described as essentially similar when they have a sequence identity of at least approximately 75%, at least approximately 80%, at least approximately 85%, more specifically at least approximately 90%, especially at least approximately 95%, more specifically at least approximately 98%, or at least approximately 99%. It is clear that when RNA sequences are said to be essentially similar or to have a certain degree of sequence identity with DNA sequences, thymidine (T) in the DNA sequence is considered equivalent to uracil (U) in the RNA sequence. Furthermore, it is clear. ML / a / ZUZZ / UU l U14 that small differences or mutations may appear in the DNA sequences over time and that some mismatches may be allowed for event-specific primers or probes of the invention, so that any DNA sequence indicated herein in any embodiment of this invention for any 3' or 5' flanking DNA or for any insert or foreign DNA or any primer or probe of this invention, also includes sequences essentially similar to the sequences provided herein, such as sequences that hybridize with at least 90%, 95%, 96%, 97%, 98%, or at least 99% sequence identity with the given sequence for any 3' or 5' flanking DNA, for any primer or probe, or for any insert or foreign DNA of this invention. The term primer, as used herein, encompasses any nucleic acid capable of priming the synthesis of a nucleic acid resulting from a template-dependent process, such as PCR. Typically, primers are oligonucleotides of 10 to 30 nucleotides, but longer sequences can be used. Primers may be provided in double-stranded form, although single-stranded form is preferred. Probes can be used as primers, but they are designed to bind to the target DNA or RNA and are not required in an amplification process. The term "recognize" as used herein refers to specific primers. It refers to the fact that the specific primers hybridize specifically to a nucleic acid sequence in the Elite Event under the conditions established in the method (such as PCR protocol identification conditions), whereby specificity is determined by the presence of positive and negative controls. The term hybridization, as used herein when referring to specific probes, refers to the fact that the probe binds to a specific region in the Elite Event nucleic acid sequence under standard rigorous conditions. Standard rigor conditions as used herein refer to the hybridization conditions described herein or to the conventional hybridization conditions described by Sambrook et al., 1989 (Molecular Cloning: A Laboratory Manual, Second Edition, Coid Spring Harbor Laboratory Press, NY) which may comprise the following steps: 1) immobilizing plant genomic DNA fragments on a filter, 2) pre-hybridizing the filter for 1 to 2 hours at 42°C in 50% formamide, 5X SSPE, 2X Denhardt's reagent and 0.1% SDS, or for 1 to 2 hours at 68°C in 6X SSC, 2X Denhardt's reagent and 0.1% SDS 3) add the hybridization probe that has been labeled 4) incubate for 16 to 24 hours 5) wash the filter for 20 minutes at room temperature in 1X SSC, 0.1% SDS, 6) perform the filter wash three times for 20 min, each time at 68°C in 0.2 X SSC, 0.1% SDS, and 7) expose the filter for 24 to 48 hours to an X-ray film at -70°C with an intensifying screen. As used herein, a biological specimen is a sample of a plant, plant material, or products comprising plant material. The term "plant" is intended to encompass tissues of cotton plants (Gossypium hirsutum), at any stage of maturation, as well as any cells, tissues, or organs taken from or derived from any such plant, including, but not limited to, cotton. ML / a / ZUZZ / UU also includes other plant material, such as seeds, leaves, stems, flowers, roots, single cells, gametes, cell cultures, tissue cultures, or protoplasts. Plant material, as used herein, refers to material obtained from or derived from a plant. Products comprising plant material refer to food, feed, or other products produced using plant material or that may be contaminated by plant material. It is understood that, in the context of the present invention, such biological samples are analyzed to determine the presence of nucleic acids specific to EEGH7, which implies the presence of nucleic acids in the samples. Therefore, the methods referred to herein for identifying the EE-GH7 Elite Event in biological samples relate to the identification in biological samples of nucleic acids comprising the Elite Event. As used herein, "comprising" should be interpreted as specifying the presence of the indicated features, wholes, steps, reagents, or components, but does not exclude the presence or addition of one or more features, wholes, steps, or components, or groups thereof. Thus, for example, a nucleic acid or protein comprising a sequence of nucleotides or amino acids may comprise more nucleotides or amino acids than those actually cited, i.e., be embedded within a larger nucleic acid or protein. A chimeric gene comprising a functionally or structurally defined DNA sequence may comprise additional DNA sequences, such as promoter and transcription termination sequences. The present invention also relates to the development of an Elite Event EE-GH7 in cotton plants comprising this event, the plant progeny and seeds comprising the Elite Event EE-GH7 obtained from these plants, and plant cells or plant material derived from plants comprising this event. The plants comprising the Elite Event EE-GH7 can be obtained as described in Example 1. This invention also relates to the seed comprising the Elite Event EE-GH7 entered into the ATCC under reference number PTA-122856 or derivatives thereof comprising the Elite Event EE-GH7. Derivatives (of seed), as used herein, refers to plants that can be grown from such seed, the progeny resulting from crossing or backcrossing, as well as plant cells, organs, parts, tissues, cell cultures, protoplasts, and plant material thereof. Cotton plants or plant material comprising EE-GH7 can be identified according to any one of the identification protocols for EE-GH7 as described in the Examples, including the PCR Identification Protocol described for EE-GH7 in Example 2.1, real-time PCR assays as described in Example 2.2, or the TaqMan endpoint as described in Example 2.3. Briefly, the cotton genomic DNA present in the biological sample is amplified by PCR using a primer that specifically recognizes a sequence within the 5' 3' flanking sequence of EE-GH7, such as the primer with the sequences SEQ ID NO: 3, SEC. ID No. 5 or SEQ ID NO: 11, and a primer that recognizes a sequence in foreign DNA, such as the primer with the sequence SEQ ID NO: 4 or SEQ ID NO: 6, or a primer that recognizes the flanking 5' 3' sequence of EE-GH7 and the foreign DNA adjacent to it, such as the primer with the sequence SEQ ID NO: 13.DNA primers that amplify part of an endogenous cotton sequence are used as a positive control for POR amplification. If, after POR amplification, the material produces a fragment of the expected size or results in fluorescence of the expected fluorescent tag, the material contains plant material from a cotton plant hosting the EEGH7 Elite Event. Plants harboring EE-GH7 are characterized by their tolerance to glyphosate, as well as their tolerance to HPPD inhibitors, such as isoxaflutol. Cotton plants of different commercially available varieties harboring EE-GH7 are also characterized by having agronomic characteristics comparable to the corresponding commercially available non-transgenic isogenic varieties, in the absence of herbicide application. The presence of foreign DNA in the insertion region of the cotton plant genome described here has been observed to confer particularly interesting phenotypic and molecular characteristics to the plants comprising this event. An embodiment of this invention provides an Elite Event in cotton plants, obtainable by inserting 2 transgenes at a specific location in the cotton genome, said Elite Event conferring tolerance to glyphosate and to an HPPD-inhibiting herbicide such as isoxaflutol in such cotton plants, and wherein such Elite Event has an agronomic performance essentially similar to isogenic lines (as used herein, “isogenic lines” or “nearly isogenic lines” are cotton lines from the same gene pool but lacking the transgenes, such as plants from the same gene pool as the plant used for transformation, or segregating sister lines that have lost the transgenes).Specifically, the present invention provides an Elite Event in cotton plants, wherein the insertion or presence of said Elite Event in the genome of such cotton plants does not appear to cause an increase in susceptibility to disease, nor a yield penalty, a reduction in fiber quality, or an increase in lodging, compared to isogenic lines. Therefore, the present invention provides an Elite Event in cotton plants, designated as EE-GH7, which results in cotton plants that can tolerate the application of glyphosate and HPPD-inhibiting herbicides without adversely affecting the yield or fiber quality parameters of said cotton plants compared to isogenic lines, whose cotton plants do not exhibit statistically significant differences from isogenic cotton plants in their susceptibility to disease or lodging.These characteristics make the current Elite Event a valuable tool in a weed resistance management program by providing tolerance to two distinct modes of action in cotton. This document also provides a cotton plant or part thereof comprising the EE-GH7 event, wherein the representative cotton seed comprising the EE-GH7 event has been entered under ATCC accession number PTA-122856. In addition, this document provides seeds of such plants comprising said event, as well as a ML / a / ZUZZ / UU fufed a cotton product produced from said seeds, wherein said cotton product comprises the EE-GH7 event. Said cotton product may be cotton fiber or a product comprising said cotton fiber. In particular, said cotton product comprises a nucleic acid producing a diagnostic or specific amplicon for the EE-GH7 event, said amplicon comprising SEQ ID NO: 3 or 4. A method for producing a cotton product is also provided herein, comprising obtaining a cotton plant, or fiber comprising the EE-GH7 event, and producing said cotton product therefrom. Also provided is a cotton plant, which is the progeny of any of the above cotton plants, and which comprises the EE-GH7 event. Furthermore, this document provides a method for producing a cotton plant tolerant to glyphosate or isoxaflutol herbicides, comprising introducing the EE-GH7 event into the genome, particularly by crossing a first cotton plant lacking the EE-GH7 event with a cotton plant comprising EE-GH7, and selecting a progeny plant tolerant to glyphosate or isoxaflutol. A glyphosate- or isoxaflutol-tolerant plant with acceptable agronomic characteristics, and particularly acceptable fiber quality parameters, is also provided. This plant comprises a 2mEPSPS and HPPD protein and is capable of producing an amplicon diagnostic for the EE-GH7 event. Specific isolated amplicons (DNA sequence fragments) are also provided, which can be obtained using the specific detection tools described herein. Specifically, amplicons that include in their sequence a DNA fragment originating from plant DNA and a DNA fragment foreign or heterologous to the plant, such as DNA inserted into the plant genome by transformation, as defined herein, are also provided. In addition, a method is provided for controlling weeds in a field of cotton plants comprising the EE-GH7 event, or a field to be planted with such cotton plants, comprising treating the field with an effective amount of an isoxaflutol-based herbicide, wherein said plants are tolerant to said herbicide. Furthermore, this document provides DNA comprising the sequence SEQ ID NO: 1 or a sequence essentially similar to it, and any plant, cell, tissue, or seed, particularly cotton, comprising such DNA sequence, such as a plant, cell, tissue, or seed comprising EE-GH7. This document also includes any cotton plant, cell, tissue, or seed comprising the DNA sequence (heterologous or foreign to a conventional cotton plant, seed, tissue, or cell) of SEQ ID NO: 1, or comprising a DNA sequence with at least 99% or 99.5% sequence identity to the sequence SEQ ID NO: 1. A chimeric DNA is also described comprising a foreign DNA, wherein the sequence of said foreign DNA consists of the sequence SEQ ID NO: 1 from nucleotide 1218 to nucleotide 8032, flanked by a 5' flanking region and a 3' region, wherein the flanking region ML / a / ZUZZ / UU fufó The 5' flanking region immediately upstream and contiguous with said foreign DNA is characterized by a sequence consisting of the sequence SEQ ID NO: 1 from nucleotide 1 to nucleotide 1217, and wherein the flanking region 3' immediately downstream and contiguous with said foreign DNA is characterized by a sequence consisting of the sequence SEQ ID NO: 1 from nucleotide 8033 to 9328. Chimeric DNA refers to DNA sequences, including regulatory and coding sequences, that are not found together in nature. Therefore, chimeric DNA may comprise adjacent DNA regions derived from different sources or arranged differently than found in nature. For example, chimeric DNA may consist of the sequence SEQ ID NO: 1. Also provided is a transgenic cotton plant, plant cell, tissue or seed, comprising in its genome the EE-GH7 event characterized by a nucleic acid molecule comprising a nucleotide sequence essentially similar to SEQ ID NO: 1, from nucleotide 1207 to nucleotide 1228 and a nucleic acid molecule comprising a nucleotide sequence essentially similar to SEQ ID NO: 1 from nucleotide 8022 to 8043, or the complement of such sequences, as well as a cotton plant, plant cell, tissue or seed, comprising in its genome the EE-GH7 event, characterized by a nucleic acid molecule comprising a nucleotide sequence essentially similar to SEQ ID NO: 1, or the complement of such sequences. Also provided is a cotton plant, cell, tissue or seed, comprising the EE-GH7 event, characterized in the genome comprising a nucleic acid sequence with at least 80%, 90%, 95% or 100% sequence identity with SEQ ID NO: 1 from nucleotide 1207 to nucleotide 1228 and a nucleic acid sequence with at least 80%, 90%, 95% or 100% sequence identity with SEQ ID NO: 1 from nucleotide 8022 to 8043, or the complement of such sequences. The term isoxaflutol, as used herein, refers to the herbicide isoxaflutol, namely (5-cyclopropyl-4-isoxazolyl)[2-(methylsulfonyl)-4-(trifluoromethyl)phenyl]methanone], its active metabolite, diketonitrile, and any mixture or solution comprising such compounds. HPPD-inhibiting herbicides useful for application in the case of this invention are diketonitriles, for example 2cyano-3-cyclopropyl-1-(2-methylsulfonyl-4-trifluoromethylphenyl)-propane-1,3-dione and 2-cyano-1-[4-(methylsulfonyl)-2-trifluoromethylphenyl]-3-(1-methylcyclopropyl)propane-1,3-dione; other isoxazoles; and pyrazolinates, for example topramezone [i.e., [3-(4,5-dihydro-3-¡soxazol¡l)-2-methyl-4-(methylsulfon¡l)phenyl](5-hydroxy-1-methyl1 H-pyrazol-4-yl)methanone], and pyrasulfotol [(5-hydroxy¡-1,3-dimethylpyrazole-4-¡l(2-mesyl-4 trifluaromethylphenyl)methanone] or pyrazophene [2-[4-(2,4-dichlorobenzoyl)-1,3-dimethylpyrazole 5-yloxy]acetophenone]. In one embodiment of this invention, a field intended to be cultivated with cotton plants containing the EE-GH7 event can be treated with an HPPD-inhibiting herbicide, such as isoxaflutol ('IFT'), or with glyphosate, or with both, an HPPD-inhibiting herbicide and glyphosate, before sowing the cotton, which involves clearing the field of weeds, which are eliminated by the herbicide. ML / a / ZUZZ / UU fufed an HPPD inhibitor or glyphosate, thus allowing no-till practices followed by planting cotton in the same pre-treated field later (burn-down application using an HPPD-inhibiting herbicide). The residual activity of IFT will also protect cotton seedlings in the early and growing stages, preventing competition with weeds. Once the cotton plants reach a certain size and weeds reappear, glyphosate, or an HPPD inhibitor-glyphosate mixture, can be applied as a post-emergence herbicide to the tops of the plants. In another embodiment of this invention, a field sown with seeds containing the EE-GH7 event can be treated with an HPPD-inhibiting herbicide, such as IFT, before the cotton plants emerge but after sowing (the field may be cleared of weeds before sowing using other means, typically conventional tillage practices such as plowing, tray plowing, or seedbed preparation). The residual activity of the herbicide will keep the field free of weeds eliminated by the herbicide, so that the emerging and growing cotton plants do not compete with weeds (pre-emergence application of an HPPD-inhibiting herbicide). Once the cotton plants reach a certain size and weeds begin to reappear, glyphosate, or an HPPD-glyphosate mixture, can be applied as a post-emergence herbicide to the upper portion of the plants. In another embodiment of this invention, plants containing the EE-GH7 event can be treated with an HPPD-inhibiting herbicide, such as IFT, on the upper portion of the cotton plants that emerged from the sown seeds, clearing the field of weeds eliminated by the HPPD inhibitor, the application of which can be in conjunction with (e.g., in a spray tank mixture), followed or preceded by a treatment with glyphosate as a post-emergence herbicide on the upper part of the plants (post-emergence application of an HPPD-inhibiting herbicide (with or without glyphosate)). Furthermore, according to the present invention, cotton plants harboring EE-GH7 can be treated with the following insecticides, herbicides, or fungicides, or cotton seeds harboring EE-GH7 can be coated with a layer comprising the following insecticides, herbicides, or fungicides. Cotton Herbicides: Carfentrazone, Clethodim, Diuron, Fluazifop-butyl, Flumioxazin, Fluometuron, Glufosinato, Glifosato, Isoxaflutol, MSMA, Norflurazon, Oxyfluorfen, Pendimethalin, Prometryn, Pyrithiobac de sodio, Tepraloxydim, Thidiazuron, Trifloxysulfuron, Trifluralin. Insecticidas de Algodón: Abamectin, Acefato, Acetamiprida, Aldicarb, Azadirachtin, Bifenthrin, Clorantraniliprol (Rynaxypyr), Chlorpyrifos, Clotianidina, Ciantraniliprol (Cyazypyr), (beta-)Cyflutrina, gamma-Cyhalothrin, lambdaCyhalothrin, Cypermethrin, Deltamethrin, Diafenthiuron, Dinotefuran, Emamectin-benzoate, Flonicamida, Flubendiamida, Fluensulfona, Fluopyram, Flupyradifurona, Imicyafos, Imidacloprida, Indoxacarb, ινΐΛ / a / zuzz / uu / u / j Metaflumizona, Pymetrozine, Piridalil, Pyrifluquinazon, Spinetoram, Spinosad, Spiromesifen, Spirotetramat, Sulfoxaflor, Tiacloprida, Tiamethoxam, Thiodicarb, Triflumuron, 1-(3-chloropind¡n-2-yl)-N-[4cyano-2-methyl-6- (methylcarbamoyl) phenyl]-3-{[5-(trifluoromethyl)-2H-tetrazol-2-yl]methyl}-1H-p¡razol-5carboxamida, 1 -(3-chloropyridin-2-iI) -N-[4-cyano-2-methyl-6-(methylcarbamoyl)phenyl]-3-{[5-(trifluoromethyl)-l Htetrazol-1-¡l]methyl}-1H-pyrazol-5-carboxamida, 1 -{2-fluoro-4-methyl-5-[(2,2,2-trifluorethyl) sulfinyl]phenyl}-3(trifluoromethyl)-1H-1,2,4-triazol-5-am¡na, (1E)-N-[(6-chloropyridin-3-yl)methyl]-N'-c¡ano-N-(2,2-difluoroethyl) etanimidida, Bacillus firmus, Bacillus firmus cepa 1-1582, Bacillus subtilis, Bacillus subtilis cepa GB03, Bacillus subtilis cepa QST 713, Metarhizium anisopliae F52. Fungicides of Cotton: Azoxystrobin, N-[9-(dichloromethylene)-1,2,3,4-tetrahydro-1,4-methanonaphthalen-5-yl]-3-(difluoromethyl)-1methyl-1H-pyrazole-4-carboxamide (Benzovindiflupyr, Benzodiflupyr), Bixafen, Boscalid, Carbendazim, Chlorothalonil, Copper, Cyproconazole, Dimoxystrobin, Epoxiconazole, Fenamidone, Fluazinam, Fluopartam, Fluxastrobin, Fluxapyroxad, Ipconazole, Iprodione, Isopyrazam, Isotianil, Mancozeb, Maneb, Mefenoxam, Metalaxil, Metominostrobin, Pencycuron, Penflufen, Penthiopyrad, Picoxystrobin, Propineb, Protioconazole, Pyraclostrobin, Quintozene, Sedaxan, Tebuconazole, Tetraconazole, Methyl-Thiophanate, Triadimenol, Trifloxystrobin, Bacillus firmus, Bacillus firmus strain 1-1582, Bacillus subtilis, Bacillus subtilis cepa GB03, Bacillus subtilis cepa QST 713. The following examples describe the development and identification of the Elite Event EE-GH7, the development of different cotton lines comprising this event, and the development of tools for the specific identification of the Elite Event EE-GH7 in biological samples. Unless otherwise stated in the examples, all recombinant techniques are carried out in accordance with the standard protocols described in “Sambrook J and Russell DW (eds.) (2001) Molecular Cloning: A Laboratory Manual, 3rd Edition, Coid Spring Harbor Laboratory Press, New York” and in “Ausubel FA, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA and Struhl K (eds.) (2006) “Current Protocols in Molecular Biology”, John Wiley & Sons, New York”. Standard materials and references are described in Cray RDD (ed.) (1993) Plant Molecular Biology LabFax, BIOS Scientific Publishers Ltd., Oxford and Blackwell Scientific Publications, Oxford and in Brown TA, (1998) Molecular Biology LabFax, 2nd Edition, Academic Press, San Diego. Standard materials and methods for polymerase chain reactions (PCR) can be found in McPherson MJ and Moller SG (2000) PCR (The Basics), BIOS Scientific Publishers Ltd., Oxford and in PCR Applications Manual, 3rd Edition (2006), Roche Diagnostics GmbH, Mannheim or www.roche-appliedscience.com It should be understood that several parameters in any laboratory protocol, such as the PCR protocols in the following examples, may need to be adjusted to specific laboratory conditions and slightly modified to obtain similar results. For example, using a different method for DNA preparation or selecting different primers in a PCR method may require different optimal conditions for the PCR protocol. However, these adjustments will be evident. ML / a / ZUZZ / UU fufó for an expert in the technique and, in addition, are detailed in current POR application manuals. The list of sequences contained in the file named LIST. SEQ. BCS162014_ST25_ESP, which has a size of 20 kilobytes (size measured in Microsoft Windows®) and contains 13 sequences SEQ ID NO: 1 to SEQ ID NO: 13, is presented electronically and is incorporated as a reference in this document. The description and examples refer to the following sequences: SEQ ID NO: 1: Foreign DNA nucleotide sequence and plant flanking sequences in EE-GH7. SEQ ID NO: 2: Plant DNA pre-insertion sequence. SEQ ID NO: 3: PRIM0728 primer SEQ ID NO: 4: primer PRIM0643 SEQ ID NO: 5: primer PRIM0638 SEQ ID NO: 6: primer PRIM0639 SEQ ID NO: 7: TM1576 probe SEQ ID NO: 8: KVM157 primer SEQ ID NO: 9: KVM158 primer SEQ ID NO: 10: TM1304 probe SEQ ID NO: 11: PRIM0726 primer SEQ ID NO: 12: PRIM0733 primer SEQ ID NO: 13: PRIM0731 primer Examples 1. Transformation of Gossypium hirsutum with herbicide tolerance genes 1.1. Description of the Foreign DNA Comprising the Chimeric Genes 2mepeps and Hppdpf-W336-1Pa The EE-GH7 cotton was developed through Agrobacterium-mediated transformation using the pTSIH09 vector containing the hppdPf-W336-1Pa and 2mepsps· expression cassettes. (i) The double mutant gene 5-enol pyruvylshikimate-3-phosphate synthase (2mepsps) encodes the 2mEPSPS protein. The coding sequence for 2mepsps was developed by introducing a point mutation at positions 102 (threonine to isoleucine substitution) and 106 (proline to serine substitution) of the wild-type epsps gene cloned from maize (Zea mays) (Lebrun et al., 1997 (document WO9704103)). Expression of the 2mEPSPS protein confers tolerance to glyphosate-containing herbicides. (i) The hppdPf-W336-1Pa gene encodes the HPPD protein W336. The coding sequence hppdPf-W336-1Pa was developed by introducing a single point mutation resulting in the replacement of amino acid glycine 336 with tryptophan in the wild-type hppd gene derived from Pseudomonas fluorescens (Boudec et al., 2001, (U.S. Patent US6245968B)). Expression of the HPPD W336 protein confers tolerance to inhibitory herbicides. ΜΛ / a / ZUZZ / UU was derived from HPPD, such as isoxaflutole. The pTSIH09 plasmid is a plant transformation vector containing a chimeric gene, 2mepsps, and a chimeric gene, hppdPf-W336-1Pa, located between the right border (RB) and left border (LB) of the T-DNA. Table 1 below provides a description of the genetic elements between the right and left borders of the T-DNA. The nucleotide sequence is represented in SEQ ID NO: 1. ML / a / ZUZZ / UU fufó Table 1. Nucleotide Positions of the DNA of Ptsih09 Inserted into the Genome of the Plant (Nt 1218-8032 Of SEQ ID NO: 1) Nucleotide Positions Orientation Description and References 1277-1943 complement 3'histonAt: sequence including the 3' untranslated region of the histone H4 gene from Arabidopsis thaliana (Chabouté et al., 1987, Plant Molecular Biology, 8, 179-191) 1960-3036 complement hppdPf W336-1Pa: sequence encoding the 4-hydroxyphenylpyruvate dioxygenase of the A32 strain of Pseudomonas fluorescens modified by the substitution of amino acid Glycine 336 by a tryptophan, as described by Boudec et al. (2001) U.S. Patent US6245968B1 3037-3408 complement TPotp Y-1Pa: Sequence encoding an optimized transit peptide derivative (position 55 changed to tyrosine), containing the sequence of the RuBisCO small subunit genes of Zea mays (maize) and Helianthus annuus (sunflower), as described by Lebrun et al. (1996) US5510471 3417-3929 complement Pcsvmv XYZ: Sequence including the promoter region of cassava vein mosaic virus (Verdaguer et al., (1996) Plant Mol Biol, 31, 1129). 4028-4944 Ph4a748 ABC: Sequence including the promoter region of the histone H4 gene of Arabidopsis thaliana (Chabouté et al., 1987, Plant Molecular Biology, 8, 179-191). 4984-5449 intronl h3At: first intron of the histone H3.III variant gene II of Arabidopsis thaliana (Chabouté et al., 1992) Journal of Molecular Biology, 225, 569-574. 5463-5834 TPotp C: Sequence encoding the optimized transit peptide, containing the sequence of the small RuBisCO subunit genes of Zea mays (maize) and Helianthus annuus (sunflower), as described by Lebrun et al. (1996) US5510471 5835-7172 2mepsps: Sequence encoding the mutant 5-enol-pyruvilshikimate-3-phosphate synthase gene of Zea mays (maize) (Lebrun et al., 1997) WO9704103-A 1 7193-7859 3'histonAt: Sequence including the 3' untranslated region of the histone H4 gene of Arabidopsis thaliana (Chabouté et al., 1987) Plant Molecular Biology, 8, 179-191. 1.2. EE-GH7 Event The pTSIH09 T-DNA vector was introduced into Agrobacterium tumefaciens C58C1 Rif (pEHA101) and cotton was selected using spectinomycin and streptomycin according to methods known in the art. Agrobacterium strains were used to transform the Coker 312” 15 cotton variety according to known methods, and the transgenic plants were selected in vitro for glyphosate tolerance (1.0–1.5 mM), followed by cell regeneration of the transformed plants into fertile transgenic cotton plants. T0 plants were treated with tembotrione (HPPD-inhibiting herbicides) to select for the expression of the hppdPfw3361Pa gene. Surviving plants were self-pollinated to produce T1 seeds. Subsequent generations T2 through T7 were obtained through self-pollination. Subsamples of T1 and T2 plants were sprayed with glyphosate to ensure the expression of the 2mepsps gene in these generations. In the T3 through T7 generations grown in the field, each self-generated generation was sprayed with glyphosate to ensure the expression of the 2mepsps gene. 1.2.1 Identification of the Elite Event EE-GH7 The Elite Event EE-GH7 was selected based on an extensive selection procedure focused on the trait's efficacy, good expression and stability of herbicide tolerance genes, and its compatibility with optimal agronomic characteristics, such as plant height, node height, boll retention, position, vigor, fiber length, fiber strength, and fiber yield. Cotton plants containing this event were selected from a wide range of transformation events obtained using the same chimeric genes.The parameters used in the selection of this event were: a) acceptable tolerance to the application of herbicide with isoxaflutol in field trials, b) acceptable tolerance to the application of herbicide with glyphosate in field trials, c) an insertion of the herbicide tolerance transgenes in a single place in the cotton genome of the plant, with absence of vector skeleton, d) general agronomic characteristics similar to those of parental plants used for transformation (maturity, lodging, susceptibility to disease, etc.).), e) absence of performance penalty or absence of changes in fiber quality properties as a result of the insertion of the transforming DNA (compared to a corresponding isogenic line without the presence of the event, such as the plant line used for transformation or commercial varieties, grown under the same conditions), f) stable inheritance of the insert and, g) phenotypic stability. 1.2.1.1 Structural Stability of the Event The structural stability of EE-GH7 was determined by Southern Blot analysis in generations T1, T3, T4, BC1F2, and BC2F3. The results of these Southern Blot analyses demonstrate the structural stability of the event in all tested generations. 1.2.1.2 Event Inheritance The inheritance of the foreign DNA insert was tested in the F2, BC1F2, and BC2F2 generations by testing the genotype of the hppdPfW336-1Pa and 2mepsps genes using POR analysis. The segregation relationships determined for three generations of EE-GH7 cotton confirmed that the hppdPfW336-1Pa and 2mepsps genes contained in the EE-GH7 insert are inherited in a predictable manner, as expected for a single insertion. These data are consistent with Mendelian principles and support the conclusion that the EE-GH7 event consists of a single insert integrated at a single chromosomal locus within the cotton nuclear genome. 1.2.1.3 Stability of Protein Expression HPPD W336 and 2mEPSPS protein expression levels were determined by an enzyme-linked immunosorbent assay (ELISA) on leaf and seed samples collected from three generations (T4, T5 and BC2F4) of EE-GH7 cotton. The mean HPPD W336 expression levels in leaves at growth stages 4-6 (BBCH 14-16) across generations T4, T5, and BC2F4 were 442.73, 421.06, and 410.89 pg / g dry weight, respectively. The mean HPPD W336 expression levels in seeds at maturity (BBCH 83-97) across the three generations were 42.83, 42.45, and 34.97 pg / g dry weight, respectively. The mean expression levels of 2mEPSPS in the leaf at growth stage 4-6 (BBCH 14-16) across generations T4, T5, and BC2F4 were 1078.03, 1115.46, and 1498.30 pg / g PS, respectively. The mean expression levels of 2mEPSPS in seeds at maturity stage (BBCH 83-97) in the three generations (T4, T5, and BC2F4) were 163.07, 160.49, and 147.80 pg / g PS, respectively. HPPD W336 and 2mEPSPS, respectively, showed similar mean expression levels in leaves and seeds across the three generations. Therefore, the stability of HPPD, W336, and 2mEPSPS protein expression was demonstrated over three generations. 1.2.1.4 Agronomic Performance and Tolerance to Isoxaflutol (IFT) and Glyphosate (GLY) In agronomic equivalence trials, the plants comprising EE-GH7 behaved similarly to the null segregants and their wild-type counterparts. The EE-GH7 plants exhibited normal agronomic characteristics compared to the corresponding non-transgenic plants. The tolerance to IFT, glyphosate, and combinations thereof of the plants comprising EE-GH7 was tested at different locations in the field. The plants comprising EE-GH7 showed good tolerance to IFT alone, particularly when IFT was applied prior to radicle emergence. The plants comprising EE-GH7 also showed good tolerance to glyphosate applied after emergence and to a combination of IFT and glyphosate, especially when IFT was applied before emergence and glyphosate was applied after emergence. Importantly, the glyphosate tolerance of the plants comprising EE-GH7 is equal to or greater than the glyphosate tolerance of the glyphosate-tolerant Elite Event EE-GH3 of WO2007 / 017186.Currently available data appear to indicate a performance equal to or greater than the performance of plants comprising EE-GH7 over plants comprising the chimeric genes hppd and epsps as described in WO2013 / 026740. To evaluate the agronomic performance of GHB811 cotton under field conditions representative of commercial cultivation, a multi-site field evaluation was conducted. The agronomic evaluation included 15 locations (seven sites in one year and eight sites in another year) representative of various cotton-producing regions. All plots within a field site were subjected to the same conditions of ML / a / ZUZZ / UU was used for growth and management (i.e., cultivation, irrigation, fertilizer, maintenance pesticide treatments). Each plot within each field trial was the same size. The EE-GH7 cotton plots, treated with the herbicide specific to each trait, received one application of each specific herbicide as a spray. IFT was applied at a rate of 100.3 to 115.2 grams of active ingredient per hectare (g ai / ha) before or shortly after emergence (BBCH 00 to 13). GLY was applied at a rate of 1067 to 1222 g ai / ha at the six- to nine-leaf growth stage (BBCH 16 to 19). The following agronomic parameters were measured during the growing season at each field trial site. Data are presented for each individual plot at each field trial. Continuous Parameters: • Seedling emergence count • Percentage of soil cover • Days and heat units to first flower appearance. • Days and heat units to the opening of the first capsules. • Percentage of open bolls • Final plant count • Boll properties • Cottonseed yield • Cotton lint yield • Binding properties (fiber) Categorical Parameters: • Abiotic stress assessment • Disease stress assessment • Insect stress assessment • Capsule type • Plant lodging Agronomic observations for the non-GM homologue (Coker 312) were compared with those of EE-GH7 cotton not treated with IFT and GLY, as well as with EE-GH7 cotton treated with IFT and GLY. Statistically significant differences were detected for the continuous parameters: final plant count, cotton seed yield, cotton lint yield, and height-to-node ratio, between the non-GM counterpart (Coker 312) and untreated EE-GH7 cotton. Statistically significant differences were also detected in boll weight between the non-GM counterparts and the two EE-GH7 cotton variants (treated and untreated). All average values for the continuous agronomic parameters of EE-GH7 cotton (treated or untreated) fell within the range of the reference varieties. Therefore, the statistically significant differences were not considered biologically relevant. VIA / a / ¿U4¿ / UU l U13 Statistical analysis of the combined site results was performed for the following categorical parameters: boll type, lodging, four insect stress indices, four disease stress indices, and four abiotic stress indices. No statistically significant differences, as defined by CMH test p-values <0.05, were detected for thirteen of the fourteen categorical parameters. Statistically significant differences were observed for the third disease stress index between the non-genetically modified counterparts and both EE-GH7 cotton variants (treated and untreated). All mean values for EE-GH7 cotton (treated or untreated) for the third disease stress index fell within the range of the reference varieties, and therefore, the statistically significant differences were not considered biologically relevant. Based on the agronomic evaluation, the EE-GH7 cotton showed no biologically relevant differences compared to the non-GM counterparts and showed an agronomic performance equivalent to the non-GM reference varieties. 1.2.2 Identification of Flanking Regions and Foreign DNA from the Elite EEGH7 Event The sequence of the regions flanking the foreign DNA comprising the herbicide tolerance genes in the Elite Event EE-GH7 was determined to be as follows: 1.2.2.1 Right Flanking Region (5') The identified fragment comprising the 5' flanking region was sequenced and its nucleotide sequence is represented in SEQ ID NO: 1, nucleotides 1-1217. 1.2.2.2 Left Flanking Region (3') The identified fragment comprising the 3' flanking region was sequenced and its nucleotide sequence is represented in SEQ ID NO: 1, nucleotides 8033-9328. 1.2.2.3 Foreign DNA Comprising the Herbicide Tolerance Genes of EE-GH7 The confirmed complete sequencing of the foreign DNA and flanking DNA sequences in EE-GH7 resulted in the sequence reported in SEQ ID NO: 1. The foreign DNA sequence of the Elite Event EE-GH7 comprising the herbicide tolerance genes is represented in SEQ ID NO: 1, nucleotides 1218-8032. This foreign DNA is immediately preceded upstream and contiguous to the foreign DNA by the 5' flanking sequence of SEQ ID NO: 1 from nucleotide 1 to 1217 and immediately followed downstream and contiguous to the foreign DNA by the 3' flanking sequence of SEQ ID NO: 1 from nucleotide 8033 to nucleotide 9328. 1.2.2.4 Identification of the DNA of the Pre-insertion Plant The DNA from the pre-insertion plant was amplified by PCR. The nucleotide sequence of the amplified fragment was identified (SEQ ID NO: 2). Nucleotides 1218–1230 of SEQ ID NO: 2 were deleted from the EE-GH7 transgenic locus (target site deletion). Nucleotides 1–1217 of SEQ ID NO: 2 correspond to the 5' flanking sequence of EE-GH7, and nucleotides 1231–2526 of SEQ ID NO: 2 correspond to the 3' flanking sequence of EE-GH7. ML / a / ZUZZ / UU l U l ó 2. Development of Identification Protocols for EE-GH7 2.1. Polymerase Chain Reaction for the Detection of the Specific Sequence of the EE-GH7 Event 2.1.1 Primers Specific primers were developed that recognize sequences within the Elite Event. A primer was developed that recognizes a sequence within the 5' flanking region of EEGH7. A second primer was then selected from within the foreign DNA sequence, so that the primers spanned a sequence of approximately 126 nucleotides. The following primers were found to give clear and reproducible results in a PCR reaction on EE-GH7 DNA: Direct primer aimed at the 5' flanking sequence: PRIM0728: 5'- CTCCgAATAgTTCCATCAATTTTATCA -3'(SEQ ID NO: 3) Reverse primer targeting foreign DNA: PRIM0643: 5'- TgATCgggCCTTAATTAACCC -3'(SEQ ID NO: 4) Preferably, an appropriate taxon-specific reference system reaction should be performed on identical quantities of DNA from all analyzed samples to demonstrate that the samples are, in principle, functional for PCR analysis. This taxon-specific reference system may consist of primers targeting an endogenous sequence that are included in the PCR mixture. These primers serve as an internal control in unknown samples and as a positive DNA control. A positive result with the endogenous primer pair demonstrates that the samples are, in principle, functional for PCR analysis and that the DNA is of adequate quality for preparing genomic DNA to generate a PCR product. 2.1.2 Amplified Fragments The expected amplified fragments in the PCR reaction are: For the pair of primers PRIM0728- PRIM0643: 126 bp (Elite Event EE-GH7) 2.1.3 Template DNA The template DNA was prepared using the AGOWA Maxi sbeadex Plant Kit. When using DNA prepared by other methods, a test should be performed using different amounts of template DNA. Generally, 50 ng of template DNA produces the best results. 2.1.4 Assigned Positive and Negative Controls To avoid false positives or negatives, it was determined that the following positive and negative controls should be included in a PCR run: - Without template control (negative DNA control). This is a PCR in which no DNA is added to the reaction. When the expected result is obtained, no PCR products are observed, indicating that the PCR cocktail was not contaminated with target DNA. - A positive DNA control (a sample of genomic DNA known to contain the transgenic sequences). Successful amplification of this positive control demonstrates that the PCR was performed in ML / a / ZUZZ / UU fufó conditions that allow amplification of target sequences. - A negative DNA control (wild-type DNA control). This is a PCR in which the template DNA provided is genomic DNA prepared from a non-transgenic plant. When the expected result is obtained, amplification of the event-specific PCR product is not observed, but rather amplification of the endogenous PCR product, indicating that there is no detectable background transgenic amplification in the genomic DNA sample. 2.1.5 PCR Conditions Optimal results were obtained under the following conditions (describing the various conditions for obtaining optimal results is intended to provide examples of such conditions. Clearly, an expert in the technique could vary the conditions, reagents, and parameters, such as using other Taq polymerases, and achieve the desired results): - The PCR mixture for 25 pl reactions contains: ng of template DNA. 5.0 pl 5x Amplification Buffer (supplied by the manufacturer with Taq polymerase) - 0.25 μI 20mM dNTPs 0.7 μΙ PRIMI PRIM0728 (10 pmol / μΙ) - 0.7 μΙ PRIMI PRIM0643 (10 pmol / μΙ) 0.1 μΙ Taq DNA polymerase (5 units / μΙ) Fill with water to 25 µL - The thermocycling profile that should be followed to obtain optimal results is the following: min. to 95°C - Followed by: min. at 95°C min. at 60°C min. at 72°C During 5 cycles Followed by sec. at 92°C sec. at 60°C min. at 72°C For 30 cycles Followed by: 10 min. at 72°C 2.1.6 Aqarosa Gel Analysis To optimally visualize the PCR results, it was determined that approximately 25 μI of sample should be loaded onto a 3% agarose gel (tris-borate buffer, stained with ethidium bromide) with an appropriate molecular weight marker (e.g., 50 bp). 2.1.7 Validation of Results ML / a / ZUZZ / UU fufó It was determined that results obtained with DNA samples from transgenic plants within a single PCR run and a single PCR mix should not be acceptable unless 1) the positive DNA control shows the expected PCR product (transgenic fragment and, if included, endogenous fragment), 2) the negative DNA control is negative for PCR amplification (no fragment), and 3) the wild-type DNA control is negative for PCR amplification for the transgenic fragment and, if included, positive for the endogenous fragment. Following the PCR Identification Protocol for EE-GH7 as described above, lanes showing visible amounts of transgenic PCR products of the expected sizes indicate that the corresponding plant from which the template DNA was prepared has inherited the EE-GH7 Elite Event. Lanes showing no visible amounts of the transgenic PCR product indicate that the corresponding plant from which the template genomic DNA was prepared does not possess the Elite Event. 2.1.8 Use of the Discriminatory PCR Protocol for the Identification of EE-GH7 Before attempting to detect unknown products, a test run is performed with all appropriate controls. The developed protocol may require optimization for components that can differ between laboratories (template DNA preparation, Taq DNA polymerase, primer quality, dNTPs, thermocycler, etc.). Leaf material from several cotton plants, some of which included EE-GH7, was tested according to the protocol described above. Samples from the Elite Event EE-GH7 and wild-type cotton were used as positive and negative controls, respectively. Figure 2 illustrates the results obtained with the Elite Event PCR Identification Protocol for EE-GH7 on various cotton plant samples. The samples in lanes 2, 3, 4, 11, 12, and 13 represent the negative control (no template); lanes 5, 6, and 7 contain DNA from wild-type cotton plants. Lanes 8, 9, and 10 comprise samples of the EE-GH7 cotton transformation event, and lanes 1 and 14 represent the molecular weight marker (50 bp scale). It can be seen that, specifically for the EE-GH7 cotton transformation event, a 126 bp PCR fragment is produced. 2.2. Real-Time PCR Assay for the Detection of the Specific Sequence of the EE-GH7 Event 2.2.1 Real-Time PCR Assay for the Detection of EE-GH7 in Seeds Flour A real-time PCR assay is established to detect the presence of low levels of EEGH7 in seeds in flour. The following primers were used in this PCR reaction: Forward primer directed at the 5' flanking sequence: PRIM0638 5'- CgAATAgTTCCATCAATTTTATCATTTATg -3' (SEQ ID NO: 5) ινΐΛ / a / zuzz / uu iui or Reverse primer targeting the foreign DNA sequence: PRIM0639 5'- TCgggCCTTAATTAACCCg - 3' (SEQ ID NO: 6) The expected amplified fragment in the PCR reaction of these primers is 120 bp. Probe directed to the flank of the 5' junction - foreign DNA sequence: TM1576 5'- AgAACAACAgTACTgggC -3'(SEQ ID NO: 7) The TM1576 probe is labelled FAM at the 5' end and with the non-fluorescent switch MGBNFQ at the 3' end. The target PCR reaction is performed on approximately 200 ng of template DNA prepared with the AGOWA Maxi sbeadex Plant Kit. When using DNA prepared by other methods, a test should be performed using samples with known relative levels of EE-GH7. An appropriate reaction of the taxon-specific reference system must be performed on identical quantities of DNA from all analyzed samples to demonstrate that the samples are in principle functional for PCR analysis. For unknown test samples, the PCR experiment should ideally include the appropriate positive and negative control samples, i.e.: - Without template control (negative DNA control). This is a PCR in which no DNA is added to the reaction. When the expected result (no PCR product) is obtained for both the target system and the reference reaction, it is known that the PCR mixture is not contaminated with the target DNA. - A positive DNA control (a sample of genomic DNA known to contain the transgenic sequences). Successful amplification of this positive control demonstrates that the PCR was performed under conditions that allow amplification of the target sequences. A negative DNA control (wild-type DNA control) can also be added to this PCR. In this PCR, the template DNA provided is genomic DNA prepared from a non-transgenic plant. When the expected result is obtained, amplification of the endogenous PCR product is not observed, indicating that there is no detectable transgenic amplification in a genomic DNA sample. This protocol was validated using 2x Perfecta qPCR Fastmix II, Low ROX, supplied by Quanta Bioscience (catalog number 95120). Any other reaction buffer may be used, but the procedures must be successfully validated using the appropriate set of positive and negative controls before analyzing samples of unknown content. Optimal results are obtained under the following conditions: - The PCR mixture for 20 pl reactions contains: 200 ng template DNA μΙ 2x Perfecta qPCR Fastmix II, Low ROX (Quanta Bioscience) - 0.5 μΙ PRIM0638 (10 pmol / pl) - 0.5 μΙ PRIM0639 (10 pmol / μΙ) ML / a / ZUZZ / UU l U14 - 0.5 μΙ ΤΜ1576 (10 pmol / μΙ) - Fill with water to 20 µL - The thermal cycle profile that should be followed to obtain optimal results is the following: min. to 95°C Followed by: 15 sec. at 95°C min. at 60°C For 40 cycles The target amplification is measured in real time by quantifying the FAM indicator dye during the 1 minute step at 60°C. The results of the PCR Identification Protocol in Real-time PCR for EE-GH7 for 1:5 dilutions of genomic DNA comprising EE-GH7 is shown in Figure 3. Figure 3 shows a clear correlation between the PCR cycle at which the threshold level is reached and the dilution of the DNA comprising EE-GH7. 2.2.2 Replication of the Real-Time PCR Assay to Determine the Presence and Zygosity of EE-GH7 in Individual Plants / Individual Seeds A replication of the real-time PCR assay is carried out to quantify the presence and zygosity of EE-GH7 in individual plants / individual seeds. The primers and probe applied in this PCR reaction directed to the transgenic DNA sequence are the same as those described for the real-time PCR assay for the detection of EEGH7 in seeds in flour as described in Example 2.2.1. Forward primer directed at the 5' flanking sequence: PRIM0638 5'- CgAATAgTTCCATCAATTTTATCATTTATg -3' (SEQ ID NO: 5) Reverse primer targeting the foreign DNA sequence: PRIM0639 5'- TCgggCCTTAATTAACCCg - 3' (SEQ ID NO: 6) The expected amplified fragment in the PCR reaction of these primers is 120 bp. Probe directed to the flank of the 5' junction - foreign DNA sequence: TM1576 5'- AgAACAACAgTACTgggC -3'(SEQ ID NO: 7) The TM1576 probe is marked with FAM at the 5' end and with the non-fluorescent switch MGBNFQ at the 3' end. The following primers, which target an endogenous sequence, are also included in the PCR mix. These primers serve as an internal control in unknown samples and as a positive DNA control. A positive result with the endogenous primer pair (presence of a PCR-amplified fragment of 74 bp) demonstrates that ample DNA of adequate quality is present in the genomic DNA preparation to generate a PCR product. Suitable endogenous primers may be primers selected to recognize a constitutive gene in cotton, such as: Forward primer targeting an endogenous sequence of a target gene: KVM157: 5'- CACATgACTTAgCCCATCTTTgC -3'(SEQ ID NO: 8) Reverse primer targeting an endogenous target gene sequence KVM158: 5'- CCCACCCTTTTTTggTTTAgC-3'(SEQ ID NO: 9) The expected amplified fragment in the PCR reaction of these primers is 74 bp. Probe targeting the endogenous target gene sequence: TM1304: 5'-TgCAggTTTTggTgCCACTgTgAATg-3'(SEQ ID NO: 10) The TM1304 probe is marked with JOE at the 5' end and with the non-fluorescent switch BHQ1 at the 3' end. This protocol was validated using 2x Perfecta qPCR Fastmix II, Low ROX supplied by Quanta Bioscience (catalog number 95120). Any other reaction buffer may be used, but the procedures must be successfully validated with an appropriate set of positive and negative controls before analyzing unknown samples. Optimal results are obtained under the following conditions: - The PCR mixture for 10 pl reactions contains: ng template DNA μΙ 2x Perfecta qPCR Fastmix II, Low ROX (Quanta Bioscience) 0.5 μΙ PRIM0638 (10 pmol / μΙ) 0.5 μΙ PRIM0639 (10 pmol / μΙ) 0.5 μΙ of KVM157 (10 pmol / μΙ) - 0.5 μΙ of KVM158 (10 pmoles / μΙ) 0.1 μΙΤΜ1576(10 pmol / μΙ) - 0.1 μΙΤΜ1304 (10 pmol / μΙ) Fill with water to a level of 10 µL. - The thermocycling profile that should be followed to obtain optimal results is the following: min. to 95°C - Followed by: sec.a 95°C 0 sec. at 60°C For 35 cycles The amplification of the target is quantified in real time by measuring the FAM dye during the 30-second step at 60°C, and the amplification of the endogenous control is measured in real time by measuring the JOE indicator dye during the 30-second step at 60°C. To avoid false positives or negatives, it was determined that the following positive and negative controls should be included in a PCR run: - Homozygous control: a genomic DNA sample containing the described target sequence ML / a / ZUZZ / UU fufó in a homozygous manner. - Hemizygous control: a genomic DNA sample containing the described target sequence in a hemizygous manner - Wild type control (negative DNA control): a genomic DNA sample that does not contain the described target sequence - Without mold control (NTC): a water sample Data analysis was performed using the ddCt method. In this method, the zygosity for each test sample is calculated relative to a reference sample. For hemizygous DNA samples for EE-GH7, a copy number of 1 was calculated, while for homozygous DNA samples for EE-GH7, a copy number of 2 was calculated using this method, demonstrating that it can be used to determine EE-GH7 zygosity status. 2.3. Endpoint TaqMan for Detection of the Specific Sequence of the EEGH7 Event The TaqMan endpoint for EE-GH7 detection uses the same primers and probes as the real-time PCR assay replica for EE-GH7 detection in individual plants / individual seeds, as described in 2.2.2. - The PCR mixture for 10 pl reactions contains: ng de ADN molde µI 2x Perfecta qPCR Fastmix II, bajo (Quanta Bioscience, No. de cat. 95119) - 0.5 μL PRIM0638 (10 pmoles / μL) - 0.5 μL PRIM0639 (10 pmoles / μL) 0.04 μΙ of KVM157 (10 pmoles / μΙ) - 0.04 μΙ of KVM158 (10 pmoles / μΙ) - 0.1 μl TM1576 (10 pmoles / μl) 0.05 μl TM1304 (10 pmoles / μl) Add water up to 10 μΙ - El thermocyclado fue el siguiente: min. at 95°C - Followed by: seg.a 95°C seg.a 60°C For 35 cycles The results for an endpoint TaqMan PCR Identification Protocol for the detection of EE-GH7 are shown in Figure 4. Samples comprising EE-GH7 (samples A1-A8) are shown to exhibit the EE-GH7 signal as well as the endogenous control signal above the threshold; samples containing untransformed cotton (samples A9 and A10) contain only the endogenous control, but not the EE-GH7 signal above the threshold, and the mold-free control (samples A11-A12) ML / a / ZUZZ / UU fufó does not present any signal above the threshold. 3. Protocol for PCR-Based Determination of EE-GH7 Cycosity Status 3.1. Primers Two primers that recognize the nucleotide sequences of the wild-type locus prior to the Elite Event insertion were designed to face each other. One primer is located at the 5' or 3' flanking region of the event, and the other is directed to the binding sequence of the target site deletion at the pre-insertion locus and the 3' or 5' flanking region, respectively. A third primer is included that is directed to the foreign DNA binding sequence at the transgenic locus and the 3' or 5' flanking region, respectively. The presence of these three primers allows for the simultaneous amplification of the EE-GH7-specific sequence by PCR, as well as the wild-type sequence. The following primers were found to give particularly clear and reproducible results in a PCR reaction with zygosity scoring on EE-GH7 DNA: PRIM0726: 5'- CAAACTCCgAATAgTTCCATCAATTT-3'(SEQ ID NO: 11) (target: plant DNA of the 5' flanking sequence) PRIM0733: 5'-gAAggTCggAgTCAACggATTgCTTACTTAAgCATTgTTCTgTTTCAAg-3'(SEQ ID NO:12) (target: 5' flank site deletion junction sequence (nt 22-49 of SEQ ID NO: 12) extended with a 5' tail (nt 1-21 of SEQ ID NO: 12)) PRIM0731: 5'-gAAggTgACCAAgTTCATgCTggCCCAgTACTgTTgTTCTgTTTC-3' (SEQ ID NO: 13) (target: flanking DNA binding sequence 5' (nt 22-45 of SEQ ID NO: 13) extended with a 5' tail (nt 1-21 of SEQ ID NO: 13)). 3.2. Amplified Fragments The expected amplified fragments in the PCR reaction are: For the primer pair PRIM0726- PRIM0733: 76 bp (wild-type locus) For the primer pair PRIM0726- PRIM0731: 71 bp (locus EE-GH7) 3.3. Template DNA The template DNA was prepared using the AGOWA Maxi sbeadex Plant Kit. When using DNA prepared by other methods, a test should be performed using different amounts of template DNA. Between 5 and 80 ng of template DNA can be used. 3.4. Assigned Positive and Negative Controls To avoid false positives or negatives, it is recommended that the following positive and negative controls be included in a PCR run: - Homozygous control: a genomic DNA sample containing the described target sequence ΜΛ / a / ZUZZ / UU was homozygous. - Hemizygous control: a genomic DNA sample containing the described target sequence in a hemizygous manner - Wild type control (negative DNA control): a genomic DNA sample that does not contain the described target sequence. - Without Mold Control (NTC): a water sample. 3.5. PCR conditions Optimal results were obtained under the following conditions. Obviously, other Taq polymerases can be used, and the conditions may differ depending on the supplier's recommendations. - The PCR mixture for 10 μI reactions contains: χ μI template DNA (20 ng) 5.0 μI of KASPar v3.0 reagent (96-384 pocili formulation) (LGC) 0.14 μL of reaction mixture - Add water up to 25 µL The 100 μL reaction mixture contains: - 12 μΙ PRIM0731 (100 pmol / μΙ) μΙ PRIM0733 (100 pmol / μΙ) μΙ PRIM0726 (100 pmol / μΙ) - Add water up to 100 µL The KASPar v3.0 reagent contains a FRET cassette labeled with a VIC dye corresponding to the tail of PRIM0733 (specific wild type primer), and a FRET cassette labeled with a FAM dye corresponding to the tail of PRIM0731 (specific primer EE-GH7). - The thermocycling profile that should be followed to obtain optimal results is the following: 15 min. at 94°C Followed by: sec. at 94°C min. at 65°C (-0.8°C / cycle) For 10 cycles Followed by: sec. at 94°C min. at 57°C During 26 cycles 3.6. Data Analysis For all samples, the fluorescent signal-to-background (S / B) ratio is calculated for both natural locus and event-specific reactions. The background level is determined by the MA / a / ¿U¿¿ / UU fufó samples NTC. The results of the test samples are only valid if the control samples yield the expected results, that is: - The homozygous control is scored as homozygous. - Hemizygous control is scored as hemizygous. - Wild-type control is scored as wild-type. - NTCs only show fluorescent background levels. A sample is scored: - Homozygous: if the sample is in the homozygous group and the S / B exceeds the minimum acceptance of S / B (i.e., the FAM S / B ratio). - Hemizygous: if the sample is located in the hemizygous group and the S / B exceeds the minimum acceptance of the S / B ratio (i.e., the FAM and VIC S / B ratio). - Wild type: if the sample is located in the wild type group and the S / B exceeds the minimum acceptance S / B ratio (i.e., the VIC S / B ratio). - “Not conclusive”: if the sample is located between the 3 groups or if the S / B ratio is lower than the minimum acceptance S / B ratio. Figure 5 shows an example of S / B ratios for homozygous EE-GH7 line material, hemizygous EE-GH7 line material, and wild-type line material. It can be seen that VIC is determined only in wild-type material (located in the wild-type group and the S / B exceeds the minimum acceptance S / B ratio), both VIC and FAM are measured in hemizygous EE-GH7 material (located in the hemizygous group and the S / B exceeds the minimum acceptance S / B ratio), and only FAM is measured in homozygous EE-GH7 material (located in the homozygous group and the S / B exceeds the minimum acceptance S / B ratio). 4. Introduction of EE-GH7 in Preferred Cultivars The Elite Event EE-GH7 was introduced through repeated crosses into the cotton variety ST 457. Agronomic performance was determined for EE-GH7 on ST 457 and Coker 312 at four different field locations. Plants were treated with 210 g of IFT (2X) before emergence, with 210 g of IFT (2X) after emergence (E; 2-4 leaves), with 4244 g of Roundup PowerMax (2X) after early emergence (E; leaf stage 2-4), mid-emergence (M; between squaring and before flowering), and late emergence (L; flowering), with 210 g of IFT + 4244 g of Roundup PowerMax (2X) after emergence, or with 210 g of IFT pre-emergence followed by 4244 g of Roundup PowerMax after emergence. The agronomic parameters were compared with the respective untreated plants with the same background but which did not include EEGH7.For a single treatment, significant differences in cotton lint yield were observed between untreated control plants and plants comprising EE-GH7 (210 g IFT + 4244 g Roundup PowerMax (2x) post-emergence). However, these results were due to a difference observed at only one site; no differences were found at the other four locations. MA / a / ¿U¿¿ / UU l U14 significant differences were found between untreated control plants and plants with the same genetic makeup but not comprising EE-GH7. For all other treatments, cotton lint yield in plants comprising EE-GH7 was not significantly different from the lint yield of untreated control plants. The above shows that in Coker 312 and ST 547, the cotton lint yield of plants comprising EE-GH7 treated with different herbicide combinations is highly equivalent to the cotton lint yield of untreated control plants not comprising the trait. Agronomic equivalence was assessed by comparing various agronomic parameters for plants comprising EE-GH7 with null segregators to wild-type plants (not comprising EE-GH7) at four sites. For ST 457, length, POB (percentage of open capsules), LP (percentage of fluff), and SI (seed index) were not significantly different from wild-type and null segregator controls. Uniformity of the EEGH7 plants at ST 457 was significantly higher than for wild-type and null segregator controls, and fluff yield was significantly higher for the null segregator but not for the wild type. For Coker 312, some fluctuation in yield was observed, resulting in slightly lower fluff yield and percentage of fluff compared to the wild type.In comparison, this lower fluff yield and percentage of fluff were not observed with the null segregant, indicating that it was not due to the presence of the event. No significant differences were found for any other parameters in Coker 312. In summary, these data show that plants comprising EE-GH7 behave very similarly to isogenic plants not comprising EE-GH7 for various agronomic parameters. The Elite Event EE-GH7 was repeatedly introduced into the cotton varieties 05M0201 and 11A, in which the following events were stacked: T304-40, comprising glufosinate tolerance and the CrylAb gene as described in WO2008 / 122406, GHB119, comprising glufosinate tolerance and the Cry2Ae gene as described in WO2008 / 151780, and COT102, comprising the VIP3A gene as described in WO2004 / 039986. The plants were treated with 105g of IFT (1X) pre-emergence, 210g of IFT (2X) pre-emergence, 105g of IFT (1X) at emergence, 210g of IFT (2X) at emergence, 4244g of Roundup PowerMax (2X) post-emergence (E, M and L), 210g of IFT pre-emergence followed by 4244g of Roundup PowerMax (2x) post-emergence, 1761g of Liberty (2X) post-emergence (E and M), 210g of IFT (2X) pre-emergence followed by 1761g of Liberty (2X) after emergence (E and M), or with 210g of IFT (2X) pre-emergence and 1761g of Liberty (2X) after emergence.Agronomic parameters were compared with plants of the same genetic background but excluding EE-GH7 at eight sites. For only one treatment and parameter (cotton lint length in the treatment with 210 g pre-emergence IFT followed by 4244 g Roundup PowerMax (2x) post-emergence) was cotton lint length significantly greater than the control. Cotton lint length was not significantly different between plants comprising EE-GH7 and control plants for any of the other treatments. IVIA / a / 2U22 / UU l U13 Other parameters tested (fluff yield, fineness, strength, and final plant height) showed no significant differences between plants comprising EE-GH7 and control plants across all treatments. These data demonstrate that plants with different commercial backgrounds comprising EE-GH7 perform very similarly to isogenic plants not comprising EE-GH7 for various agronomic parameters. Furthermore, the presence of EE-GH7 does not affect glufosinate tolerance (Liberty) conferred by T304-40 and GHB119, nor does it affect the expression of Bt genes (CrylAb, Cry2Ae, VIP3A). The agronomic equivalence of plants comprising EE-GH7 in the cotton varieties 05M0201m 11A and 04SC095, which comprise T304-40, GHB119, and COT102 as previously described, was compared across various agronomic parameters between plants comprising EEGH7 with null segregants and with plant material 05M0201, 11A, and 04SC095 that do not comprise EE-GH7 at four sites. Plants comprising EE-GH7 were found to behave similarly to the null segregants in the agronomic equivalence trials. El Evento Elite EE-GH7 es introducido mediante un cruce repetido de cultivares de algodón comerciales incluyendo, pero no limitado a: FM 989, FM 958, FM 966, FM 832, FM 5013, FM 5015, FM 5017, FM 958B, FM 832B, FM 989BR, FM 991BR, FM 800BR, FM 960BR, FM 5045BR, FM 960B2R, FM 989B2R, FM 991B2R, FM 800B2R, FM 958LL, FM 966LL, FM 993LL, FM 981LL, FM 832LL, FM 5035LL, FM 960B2, FM 955LLB2, FM 965LLB2, FM 988LLB2, FM 9063B2F, FM 9058F, FM 9060F, FM 9068F, AFD 5062LL, AFD 5065B2F, AFD 5064F, AFD 3070F, AFD 3074F, FM 1880B2F, FM 1600LL, FM 1800LL, FM 9150F, FM 820F, FM 840B2F, FM 835LLB2, FM 1735LLB2, FM 1740B2F, FM 9180B2F, FM 1640B2F, FM 1840B2F, FM 966B, FM 9160B2F, FM 1845LLB2, ST4288B2F, ST5288B2F, FM 1773LLB2, FM 9101GT, FM 9103GT, FM 9250GL, FM 9170B2F, FM 2011GT, FM 2989GLB2, ST 4145LLB2, FM 2484B2F, FM 1944GLB2, FM 8270GLB2, ST 5445LLB2, FM 1320GL, ST 4946GLB2, ST 4747GLB2, ST 6448GLB2, ST 5032GLT, FM 2322GL, FM 1830GLT, FM 2334GLT, ST 5289GLT, ST 6182GLT, ST5115GLT, FM 1900GLT,FM 2007GLT, ST4949GLT, ST4848GLT, FM 1911GLT., It was observed that the introgression of the Elite Event in these cultivars did not significantly influence any of the desirable phenotypic or agronomic characteristics of these cultivars (without carryover of undesirable genes), while the expression of the transgene, determined by tolerance to glyphosate or isoxaflutol, presented commercially acceptable levels. This confirms the status of the EE-GH7 event as an Elite Event. The Elite Event EE-GH7 can be advantageously combined with other commercially available elite events. Particularly useful transgenic plants that can be treated according to the invention are plants containing transformation events, or a combination of transformation events, and which are listed, for example, in the databases of various national or regional regulatory agencies, including event 531 / PV-GHBK04 (cotton, insect control, described in WO 2002 / 040677), event 1143-14A (cotton, insect control, not subject to change, described in WO 06 / 128569); and event 1143-51B (cotton, insect control, not subject to change, described in WO 06 / 128570); Event 1445 (cotton, herbicide tolerance, not submitted, described in US-A 2002-120964 or WO 02 / 034946); Event 281-24-236 (cotton, insect control - herbicide tolerance, submitted as PTA-6233, described in WO 05 / 103266 or US-A 2005-216969); Event 3006-210-23 (cotton, insect control - herbicide tolerance, submitted as PTA-6233, described in US-A 2007-143876 or WO 05 / 103266); Event CE43-67B (cotton, insect control, submitted as DSM ACC2724, described in US-A 2009-217423 or WO 06 / 128573); Event CE44-69D (cotton, insect control, not submitted, described in US-A 2010-0024077); Event CE44-69D (cotton, insect control, not submitted, described in document WO 06 / 128571); Event CE46-02A (cotton, insect control, not submitted, described in document WO 06 / 128572); Event COT102 (cotton, insect control, not submitted, described in documents US-A 2006-130175 or WO 04 / 039986);Event COT202 (cotton, insect control, not submitted, described in documents US-A 2007-067868 or WO 05 / 054479); Event COT203 (cotton, insect control, not submitted, described in document WO 05 / 054480); Event GHB119 (cotton, insect control - herbicide tolerance, submitted as ATCC PTA-8398, described in document WO 08 / 151780); Event GHB614 (cotton, herbicide tolerance, submitted as ATCC PTA-6878, described in documents US-A 2010-050282 or WO 07 / 017186); Event LLcotton25 (cotton, herbicide tolerance, submitted as ATCC PTA-3343, described in WO 03 / 013224 or US-A 2003-097687); Event MON15985 (cotton, insect control, submitted as ATCC PTA2516, described in US-A 2004-250317 or WO 02 / 100163); Event MON88913 (cotton, herbicide tolerance, submitted as ATCC PTA-4854, described in WO 04 / 072235 or US-A 2006-059590);Event MON88701 (cotton, herbicide tolerance, submitted as PTA-11754, described in WO 2012 / 134808), Event T304-40 (cotton, insect control - herbicide tolerance, submitted as ATCC PTA-8171, described in US-A 2010-077501 or WO 08 / 122406); Event T342-142 (cotton, insect control, not submitted, described in WO 06 / 128568); event MON88701 (cotton, accession number ATCC PTA-11754, document WO 2012 / 134808A1), event pDAB4468.18.07.1 (cotton, herbicide tolerance, accession number ATCC PTA-12456), document WO2013112525A2, event pDAB4468.19.10.3 (cotton), herbicide tolerance, accession number ATCC PTA-12457), WO2013112527A1, event A26-5 (cotton, insect control) WO2013170398, event A2-6 (cotton, insect control) WO2013170399, event A26-5 (cotton, as described in WO2013170398A1), event A2-6 (Cotton, as described in document WO2013170399A1).; Plants combining EE-GH7 with the GHB119 event (cotton, insect control herbicide tolerance, submitted as ATCC PTA-8398, described in WO 08 / 151780), the T304-40 event (cotton, insect control - herbicide tolerance, submitted as ATCC PTA-8171, described in US-A 2010-077501 or WO 08 / 122406), and the COT102 event (cotton, insect control, not submitted, described in US-A 2006-130175 or WO 04 / 039986) are particularly useful for the present invention. As used in the following claims, unless clearly stated otherwise, the term "plant" is intended to encompass plant tissues, at any stage of maturity, ML / a / ZUZZ / UU fufó as well as any cell, tissue or organ taken or derived from any of said plants, including without limitation, any seed, leaves, stems, flowers, roots, single cells, gametes, cell cultures, tissue cultures or protoplasts. The reference seed comprising the Elite Event EE-GH7 was entered into the ATCC (10801 University Blvd., Manassas, VA 20110-2209) on February 24, 2016, with ATCC accession number PTA-122856, and its viability was confirmed. The alternate name for EE-GH7 is event GHB 811. The foregoing description of the invention is intended to be illustrative and not limiting. Skilled practitioners may make various changes or modifications to the described embodiments. These may be made without departing from the objective or scope of the invention.
Claims
1. A method for producing cotton plants, characterized in that it comprises sowing cotton seed comprising the elite event EE-GH7, the reference seed comprising said event having been deposited in the ATCC with deposit number PTA-122856, in a field, and growing cotton plants from said seeds, wherein said field is treated with an HPPD-inhibiting herbicide.
2. The method of claim 1, characterized in that said field is treated with an HPPD-inhibiting herbicide, before the emergence of the cotton plants, but after the seeds have been sown.
3. The method of claim 1, characterized in that the field is treated with an HPPD-inhibiting herbicide after the emergence of the cotton plants.
4. The method of claim 1, characterized in that said field is treated with an HPPD-inhibiting herbicide prior to seed emergence.
5. A method for producing cotton plants, characterized in that it comprises sowing cotton seed comprising the elite event EE-GH7, the reference seed comprising said event having been deposited in the ATCC with deposit number PTA-122856, in a field, and growing cotton plants from said seeds, wherein said field is treated with glyphosate after the emergence of the cotton plants.
6. The method according to claim 5, characterized in that said field is further treated with an HPPD-inhibiting herbicide after the emergence of the cotton plants.
7. The method according to claim 6, characterized in that said field is treated with a mixture of HPPD inhibitor herbicide and glyphosate after the emergence of the cotton plants.
8. The method according to claim 1, characterized in that said HPPD-inhibiting herbicide is a diketonitrile, an isoxazole, a pyrazolinate, pyrasulfothol, or pyrazophen.
9. The method according to claim 8, characterized in that said HPPD-inhibiting herbicide is topramezone.
10. The method according to claim 8, characterized in that said HPPD inhibitor herbicide is isoxaflutol.
11. A method for producing a cotton product, characterized in that it comprises producing the cotton plants according to claim 1, and producing a cotton product from said cotton plants.
12. The method according to claim 11, characterized in that said cotton product is fiber, cotton lint, seed, seed meal or seed oil.
13. A method for weed control in a field where cotton seed comprising the elite event EE-GH7 is sown, reference seed comprising said event having been deposited in the ATCC under deposit number PTA-122856, or where cotton plants comprising the elite event EE-GH7, reference seed comprising said event having been deposited in the ATCC under deposit number PTA-122856, are sown and cultivated, characterized in that it comprises applying an HPPD inhibitor. 5 14. The method according to claim 13, characterized in that said HPPD inhibitor is topramezone or isoxaflutol.
15. A method for weed control in a field in which cotton seed comprising the elite event EE-GH7 is sown, reference seed comprising said event having been deposited in the ATCC with deposit number PTA-122856, or where cotton plants comprising the elite event EE-GH7, reference seed comprising said event having been deposited in the ATCC with deposit number PTA-122856, are sown and cultivated, characterized in that it comprises applying glyphosate.