Automated cell culture device

Abstract

An automated cell culture device includes a main body (10) and a clamping member (20). The main body (10) encloses a receiving space and has a platform (11) on a top face thereof. The platform (11) has a sliding track (12) extending along a sliding direction. Two ends of the platform (11) along the sliding direction are defined as a first end (101) and a second end (102) respectively. The platform (11) is adapted for support a culture bag (70) lying thereon. The culture bag (70) has a fluid inlet (71) at an end thereof closer to the first end (101) of the platform (11). The clamping member (20) is slidably disposed on the sliding track (12) and is adapted for clamping and pressing the culture bag (70) to avoid fluid in the culture bag (70) from flowing toward an end of the culture bag (70) closer to the second end (102) of the platform (11). The clamping member (20) is slidable on the culture bag (70) along the sliding direction.

Description

Automated cell culture device Technical Field

[0001] The present invention relates to a cell culture device. Background Art

[0002] Medical technology is advancing rapidly. Unlike the traditional treatment of cancer and other diseases with chemical drugs, gene therapy and cell therapy are now more commonly used in place of chemical drugs, thanks to advances in regenerative medicine. For example, immune cell therapy, which utilizes a patient's own immune cells, can more effectively attack cancer cells while avoiding compromising the patient's immune system and resulting in fewer side effects.

[0003] In order to perform cell therapy, autologous or allogeneic cells must be cultured in vitro, allowed to proliferate, and then injected into the patient. Cell culture is usually carried out in a soft culture bag. As the number of cells proliferates, new culture medium is gradually injected, and the culture medium is placed in a laboratory environment with a specific temperature and humidity.

[0004] To confirm the cell count, small amounts of culture medium must be manually extracted from the bag multiple times to count the cells. Then, the corresponding amount of culture medium must be injected based on the cell count. This step must be performed multiple times throughout the cell culture process, which is labor-intensive and carries the risk of cell contamination and variability in manual operation. This results in high costs for cell culture, making it difficult for average patients to afford it, and unstable quality.

[0005] Existing cell culture devices, such as the WAVE Bioreactor (Cytiva), use culture bags of fixed size for cell culture. In addition, since the initial number of cells is small at the beginning of the culture, the amount of culture fluid in the culture bag is also small, and the cells will be too dispersed when dispersed throughout the culture bag. It is known that the interaction between cells contributes to cell growth. If the number of cells per unit area is too low during the culture process, the interaction will be reduced, which will lead to slow cell proliferation rate or uneven growth. These problems still need to be improved. In addition, in order to solve the problem of too low a number of cells per unit area due to a small number of initial cells, the solution in the past was to replace the culture container. For example, a smaller container was used in the early stage of culture so that the cells would not be too dispersed in the container. As the number of cells increased, a larger container was replaced. However, the above-mentioned method of replacing the culture container may increase the risk of cell contamination and it is difficult to achieve the goal of full automation of cell culture. It still needs to be improved.

[0006] Summary of the Invention

[0007] The object of the present invention is to provide a cell culture device that can maintain the concentration of culture fluid regardless of the amount of culture fluid in the culture bag, thereby ensuring the stability of cell culture quality and facilitating automated culture production.

[0008] To achieve the above objectives, the present invention adopts the following technical means:

[0009] An automated cell culture device of the present invention comprises:

[0010] A main body enclosing a receiving space and having a top surface forming a platform, the platform having at least one slide rail portion extending along a sliding direction, with opposite ends of the platform along the sliding direction being defined as a first end and a second end, respectively. A culture bag is placed flat on the platform, wherein the culture bag has at least one liquid inlet located at an end of the culture bag closer to the first end;

[0011] A bag clamping member is slidably disposed on the at least one slide rail portion. The bag clamping member is used to press against the culture bag to prevent the liquid in the culture bag from flowing to the side of the culture bag near the second end of the platform. The bag clamping member can slide on the culture bag along the sliding direction.

[0012] Preferably, the apparatus further comprises at least one supporting mechanism, which is at least provided at the second end of the platform for clamping an end of the culture bag close to the second end of the platform.

[0013] Preferably, the at least one supporting mechanism comprises at least two clamping members, and the two clamping members are respectively disposed on opposite sides of the body to clamp opposite sides of one end of the culture bag close to the second end of the platform.

[0014] Preferably, each of the clamping members comprises two magnetic blocks, one of which is fixed to the platform, and the other is detachably attracted to the magnetic block fixed to the platform, so that a corner of the culture bag is clamped between the two magnetic blocks.

[0015] Preferably, the bag clamping member comprises two cross bars, the two cross bars are respectively perpendicular to the sliding direction, the two cross bars are spaced apart from each other, and the distances between the two cross bars and the platform are different.

[0016] Among them, preferably, there is a slide rail part on each of the opposite sides of the platform, and the bag clamping part further includes two connecting parts and two sliding parts. The opposite ends of each cross bar are respectively fixed to the two connecting parts, and the two connecting parts are respectively detachably arranged on the two sliding parts, and the two sliding parts are respectively slidably arranged on the two slide rail parts.

[0017] Among them, preferably, it further includes a mobile driving mechanism, which is arranged in the accommodating space of the main body and includes a belt group and two magnetic parts. The two magnetic parts are arranged on the belt group and can be driven by the belt group to move along the sliding direction. Each of the sliding parts is respectively provided with a magnetic part, and the magnetic parts of the two sliding parts are respectively magnetically attracted to the two magnetic parts, so that the belt group can drive the two sliding parts to move along the sliding direction.

[0018] Preferably, the apparatus further comprises a pushing mechanism, which is disposed in the accommodation space of the main body and located at the first end of the main body. The pushing mechanism can be lifted and lowered to reciprocally lift and reset the end of the culture bag close to the first end.

[0019] Among them, preferably, the pushing mechanism includes a rotating device, an eccentric wheel and a pushing plate. The eccentric wheel is eccentrically arranged on the rotating device and rotates eccentrically with the rotating device. The periphery of the eccentric wheel is used to abut the pushing plate, and the pushing plate is used to abut the culture bag. When the eccentric wheel rotates eccentrically, the pushing plate is reciprocatingly pushed upward and reset downward by the eccentric wheel.

[0020] Preferably, the apparatus further comprises a breathable member disposed on the platform and having a concave-convex structure on its surface. The breathable member is sandwiched between the platform and the culture bag to allow the bottom surface of the culture bag to be breathable.

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] The cell culture device provided by the present invention can maintain the concentration of culture fluid during the process of sequentially adding culture fluid, preventing the culture fluid from being dispersed throughout the culture bag, which would affect the efficiency and quality of cell culture. It can also achieve the function of regulating the number of cells per unit area in the culture bag. Moreover, it can cooperate with the culture fluid addition mechanism to achieve an automated production process that does not require manual operation. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] FIG1 is a perspective view of the present invention;

[0024] FIG2 is a schematic diagram of the use of the present invention;

[0025] FIG3 is a cross-sectional view of the present invention;

[0026] Figures 4 to 7 are cross-sectional views of the present invention when in use;

[0027] FIG8 is a comparison chart of the results of cell culture using the present invention and the prior art.

[0028] Explanation of symbols:

[0029] Body 10

[0030] First End 101

[0031] Second end 102

[0032] Platform 11

[0033] Slide rail 12

[0034] Bag clamp 20

[0035] Crossbar 21

[0036] Connector 22

[0037] Slider 23

[0038] Magnetic parts 231

[0039] Clip 30

[0040] Magnetic block 31

[0041] Magnetic block 32

[0042] Mobile drive mechanism 40

[0043] Belt set 41

[0044] Magnetic attraction portion 42

[0045] Pushing mechanism 50

[0046] Rotating device 51

[0047] Eccentric wheel 52

[0048] Push plate 53

[0049] Fixing 531

[0050] Breathable parts 60

[0051] Culture Bag 70

[0052] Liquid inlet 71

[0053] Fixing portion 72 DETAILED DESCRIPTION

[0054] 1 to 7 , the present invention provides an automated cell culture device, which mainly includes a body 10 and a bag clamping member 20 , and may further include at least a tensioning mechanism, a moving driving mechanism 40 and a pushing mechanism 50 .

[0055] The main body 10 encloses a receiving space and forms a platform 11 on the top surface. The platform 11 has a slide rail portion 12 on opposite sides. Each of the slide rail portions 12 extends along a sliding direction. The two opposite ends of the platform 11 along the sliding direction are defined as a first end 101 and a second end 102. The platform 11 is provided with a culture bag 70 placed flat on it, wherein the culture bag 70 is provided with target cells to be proliferated and culture fluid, and has at least one liquid inlet 71 for injecting culture fluid. The liquid inlet 71 is located on the culture bag 70 closer to the culture fluid. One end of the first end 101 is connected to a culture medium supply source, such as a peristaltic motor. Preferably, a breathable member 60 may be provided on the platform 11. The breathable member 60 has a concave-convex structure on its surface. The breathable member 60 is sandwiched between the platform 11 and the culture bag 70 to allow ventilation of the bottom surface of the culture bag 70. For example, in this embodiment, the breathable member 60 may be a metal mesh, a perforated plate (the surface of the perforated plate is considered to be a convex structure, and the perforations of the perforated plate are considered to be a concave structure), or other objects with similar structures.

[0056] The bag clamping member 20 is slidably disposed at opposite ends on the two slide rails 12 . The bag clamping member 20 is configured to press against the culture bag 70 to prevent liquid in the culture bag 70 from flowing toward the side of the culture bag 70 adjacent to the second end 102 of the platform 11 . For example, the bag clamping member may press against the culture bag directly toward the platform, or may clamp against the culture bag in other ways to achieve a blocking effect. The bag clamping member 20 is capable of sliding along the sliding direction on the culture bag 70 .

[0057] The at least one supporting mechanism is at least provided at the second end 102 of the platform 11 for clamping the end of the culture bag 70 close to the second end 102 of the platform 11. Preferably, the at least one supporting mechanism can be further provided at the first end of the platform for clamping the end of the culture bag close to the first end of the platform. Specifically, the at least one supporting mechanism includes at least two clamping members 30, and the two clamping members 30 are respectively provided at opposite sides of the body 10 close to the two slide rails 12 for clamping the culture bag 70 close to the second end of the platform 11. If the at least one supporting mechanism is located at the first end of the platform, the same configuration can be used on opposite sides of one end of the end 102. More specifically, in this embodiment, each clamping member 30 includes two magnetic blocks 31 and 32, one of which is fixed to the platform 11, and the other magnetic block 32 is removably magnetically attracted to the magnetic block 31 fixed to the platform 11, so as to sandwich a corner of the culture bag 70 between the two magnetic blocks 31 and 32. However, each clamping member may also be other structures, such as existing clamps. It is particularly important to note that the height of the two magnetic blocks 31 is preferably no less than that of the bag clamping member, so that the end of the culture bag corresponding to the second end of the platform is higher than the rest of the culture bag, thereby effectively preventing the culture fluid from flowing toward the second end due to gravity and capillary effects.

[0058] Regarding the bag clamping member 20, in this embodiment, it includes two cross bars 21, two connecting members 22 and two sliding members 23. The two cross bars 21 are respectively arranged horizontally perpendicular to the sliding direction. The two cross bars 21 are spaced apart from each other. The distances between the two cross bars 21 and the platform 11 are different, that is, the heights of the two cross bars 21 relative to the platform 11 are different; the opposite ends of each of the cross bars 21 are respectively fixed to the two connecting members 22, and the two connecting members 22 are respectively detachably arranged on the two sliding members 23, and the two sliding members 23 are respectively slidably arranged on the two slide rail portions 12. For example, each of the sliding members 23 may have an embedding groove for the connecting member 22 to be embedded.

[0059] The moving drive mechanism 40 is arranged in the accommodation space of the main body 10. In this embodiment, it includes a belt set 41 and two magnetic parts 42. The two magnetic parts 42 are arranged on the belt set 41 and can be driven by the belt set 41 to move along the sliding direction. Each of the sliding members 23 is respectively provided with a magnetic member 231. The magnetic members 231 of the two sliding members 23 are respectively magnetically attracted to the two magnetic parts 42, so that the belt set 41 can drive the two sliding members 23 to move along the sliding direction. In this way, the platform does not need to be provided with a penetrating slide rail, and the accommodation space of the main body can be kept in a closed state to prevent the internal parts from being affected by the external temperature and moisture and rusting.

[0060] The pushing mechanism 50 is provided in the accommodation space of the main body 10 and is located at the first end 101 of the main body 10. The pushing mechanism 50 can be lifted and lowered to reciprocate the end of the culture bag 70 close to the first end 101 to lift and reset it downward, thereby slightly shaking the culture liquid in the culture bag 70 to ensure uniform mixing. In this embodiment, the pushing mechanism 50 may include a rotating device 51, an eccentric wheel 52 and a pushing plate 53. The rotating device 51 may be driven by a motor to rotate. The eccentric wheel 52 is eccentrically mounted on the rotating device 51 and rotates eccentrically along with the rotating device 51. The peripheral edge of the eccentric wheel 52 abuts against the push plate 53, and the push plate 53 abuts against the culture bag 70. When the eccentric wheel 52 rotates eccentrically, the top height of the eccentric wheel 52 changes continuously, causing the push plate 53 to be reciprocally and continuously pushed upward and reset downward by the eccentric wheel 52. However, the pushing mechanism is not limited to the above-mentioned mechanism, and other lifting mechanisms may also be used.

[0061] In actual use, as shown in FIG4 , the end of the culture bag 70 opposite the liquid inlet 71 passes between the two crossbars 21 toward the first end 101, then straddles the upper crossbar 21 and continues toward the second end 102, where it is secured by the two clamping members 30. With this structure, the middle portion of the culture bag 70 is compressed and clamped by being wrapped between the two crossbars 21. Furthermore, the end of the culture bag 70 corresponding to the second end 102 is higher than the end corresponding to the first end 101, further preventing the culture fluid from flowing toward the second end 102. It should be noted that, in this embodiment, the "automated cell culture apparatus" of the present invention refers to one in which the bag clamping member is automatically moved. However, the present invention does not exclude the possibility of manual adjustment of the bag clamping member. This is to be noted. The culture bag 70 may have a fixing portion 72 at the end corresponding to the first end 101. The fixing portion 72 may be, for example, a sampling tube for the culture bag 70. The push plate 53 may have a corresponding fixing member 531. The fixing portion 72 may be positioned on the fixing member 531, so that both ends of the culture bag 70 are respectively positioned on the body 10. In this way, when the bag clamping member 20 moves along the sliding direction, the culture fluid in the culture bag 70 near the liquid inlet 71 is pushed toward the liquid inlet 71 to prevent the culture fluid from being dispersed throughout the culture bag 70. Even if the starting cell number is small, the number of cells per unit area in the culture bag can still reach a number that facilitates cell-to-cell contact, as shown in FIG. 5 . In this embodiment, the fixing member 531 is a fastener that can elastically deform and retain the tube. It is disposed on the push plate 53 and is used to position the fixing portion 72 of the culture bag 70. However, if the fixing member 531 is replaced with another type of clamp, such as a spring clip or a magnetic clip, and is disposed on the push plate 53 or the main body 10 near the first end 101, it can also be used to position the culture bag away from the other end of the supporting mechanism, thereby achieving similar effects as this embodiment.

[0062] During the cell culture process, new culture medium is gradually injected into the culture bag 70, and the number of culture medium and cells in the culture bag 70 gradually increases. The bag clamping member 20 can be continuously and gradually moved toward the second end 102, or the bag clamping member 20 can be moved in stages, as shown in FIG6, to release space in the culture bag. The timing, speed, and distance of the movement can be adjusted and controlled according to the desired number of cells per unit area and the amount of culture medium injected.

[0063] 5 to 7 , the pushing mechanism 50 can reciprocally push and reset the end of the culture bag 70 near the first end 101 , thereby slightly shaking the culture medium in the culture bag 70 to achieve a moderate mixing effect, thereby promoting uniform and efficient cell culture.

[0064] Regarding the actual culture effect of the cell culture device provided by the present invention, please refer to Figure 8, which shows natural killer cells isolated from peripheral blood samples of the same subject, and then cultured in vitro using three different devices or methods: (1) using the cell culture device of the present invention, (2) using the commercially available cell culture device WAVE Bioreactor, and (3) manual cell culture (i.e., without using a cell culture device). The peripheral blood samples were whole blood collected from the subjects' arms according to a plan approved by the ethics committee. The cell cultures performed in the three methods described above all used the same starting cell number, culture medium, and number of days of in vitro culture. As can be seen from FIG8 , compared to manual culture, the cell culture device of the present invention can achieve a cell expansion multiple of 80% of that achieved by manual culture on the 15th day. This shows that the cell culture device of the present invention can replace manual culture, effectively saving manpower. In addition, the experimental results of FIG8 also show that, compared to manual culture, the cell expansion multiple of the existing commercially available WAVE Bioreactor can only reach 26% of that achieved by manual culture on the 15th day, which is significantly lower than the cell expansion multiple of the cell culture device of the present invention. This shows that the cell culture device of the present invention can indeed improve the efficiency of existing cell culture devices.

[0065] Through the above-mentioned structure and operation, the present invention can be further connected and coordinated with the culture medium supply source and additional temperature, gas, pH and humidity monitoring and control mechanisms. The injection frequency, speed and injection volume of the culture medium or gas can be calculated using a model. Based on this, the movement timing and movement speed of the bag clamping member are then set. The pushing mechanism is driven at the appropriate time and frequency to shake and mix the culture medium. This allows the entire process to be controlled in an automated manner, eliminating the need for manual injection and shaking. It can also ensure that the culture medium is not dispersed, allowing cells to proliferate and grow in a constant state, thereby improving the efficiency and yield of cell culture. More importantly, it can significantly save the cost of manual operation and reduce manual operation errors.

[0066] Thus, the cell culture device provided by the present invention can significantly reduce the labor costs required while maintaining the efficiency and quality of cell culture, thereby reducing the cost of cell culture and facilitating mass production. This reduces the price of cell therapy, which was previously unaffordable, and benefits all types of patients, promoting medical welfare, which is truly what the medical industry and the general public are looking forward to. However, the above embodiments are merely for the purpose of illustrating and explaining the technical content of the present invention. The scope of protection of the present invention shall be based on the scope defined by the claims.

[0067] Exemplary embodiments

[0068] 1. An automated cell culture device, comprising:

[0069] A main body enclosing a receiving space and having a top surface forming a platform, the platform having at least one slide rail portion extending along a sliding direction, with opposite ends of the platform along the sliding direction being defined as a first end and a second end, respectively. A culture bag is placed flat on the platform, wherein the culture bag has at least one liquid inlet located at an end of the culture bag closer to the first end;

[0070] A bag clamping member is slidably disposed on the at least one slide rail portion. The bag clamping member is used to press against the culture bag to prevent the liquid in the culture bag from flowing to the side of the culture bag near the second end of the platform. The bag clamping member can slide on the culture bag along the sliding direction.

[0071] 2. The automated cell culture apparatus according to embodiment 1 is characterized in that it further comprises at least one supporting mechanism, which is provided at least at the second end of the platform to clamp one end of the culture bag close to the second end of the platform.

[0072] 3. The automated cell culture device as described in Embodiment 2 is characterized in that the at least one supporting mechanism includes at least two clamping members, and the two clamping members are respectively arranged on opposite sides of the body to clamp the culture bag on opposite sides of one end close to the second end of the platform.

[0073] 4. The automated cell culture device according to embodiment 3 is characterized in that each of the clamping members comprises two magnetic blocks, one of which is fixed to the platform, and the other of which is detachably magnetically attracted to the magnetic block fixed to the platform, so as to clamp a corner of the culture bag between the two magnetic blocks.

[0074] 5. The automated cell culture device as described in embodiment 1 is characterized in that the bag clamping member includes two cross bars, the two cross bars are respectively perpendicular to the sliding direction, the two cross bars are spaced apart from each other, and the distances between the two cross bars and the platform are different.

[0075] 6. The automated cell culture device as described in Embodiment 5 is characterized in that there is a slide rail portion on each of the opposite sides of the platform, and the bag clamping part further includes two connecting parts and two sliding parts, and the opposite ends of each cross bar are respectively fixed to the two connecting parts, and the two connecting parts are respectively detachably arranged on the two sliding parts, and the two sliding parts are respectively slidably arranged on the two slide rail portions.

[0076] 7. The automated cell culture device as described in embodiment 6 is characterized in that it further includes a mobile driving mechanism, which is arranged in the accommodating space of the main body and includes a belt group and two magnetic parts. The two magnetic parts are arranged on the belt group and can be driven by the belt group to move along the sliding direction. Each of the sliding parts is respectively provided with a magnetic part. The magnetic parts of the two sliding parts are magnetically attracted to the two magnetic parts respectively, so that the belt group can drive the two sliding parts to move along the sliding direction.

[0077] 8. The automated cell culture apparatus according to embodiment 1 is characterized in that it further comprises a pushing mechanism, which is disposed in the accommodation space of the main body and is located at the first end of the main body. The pushing mechanism can move up and down to reciprocally lift and lower the end of the culture bag adjacent to the first end.

[0078] 9. The automated cell culture device as described in embodiment 8 is characterized in that the pushing mechanism includes a rotating device, an eccentric wheel and a pushing plate. The eccentric wheel is eccentrically arranged on the rotating device and rotates eccentrically with the rotating device. The periphery of the eccentric wheel is used to abut the pushing plate, and the pushing plate is used to abut the culture bag. When the eccentric wheel rotates eccentrically, the pushing plate is reciprocally pushed upward and reset downward by the eccentric wheel.

[0079] 10. The automated cell culture apparatus according to embodiment 1 is characterized in that it further comprises a breathable member, which is disposed on the platform and has a concave-convex structure on its surface. The breathable member is sandwiched between the platform and the culture bag to allow the bottom surface of the culture bag to be breathable.

Claims

1. An automated cell culture device, characterized in that: Include: A main body enclosing a receiving space and having a top surface forming a platform, the platform having at least one slide rail portion extending along a sliding direction, with opposite ends of the platform along the sliding direction being defined as a first end and a second end, respectively. A culture bag is placed flat on the platform, wherein the culture bag has at least one liquid inlet located at an end of the culture bag closer to the first end; A bag clamping member is slidably disposed on the at least one slide rail portion. The bag clamping member is used to press against the culture bag to prevent the liquid in the culture bag from flowing to the side of the culture bag near the second end of the platform. The bag clamping member can slide on the culture bag along the sliding direction.

2. The automated cell culture device according to claim 1, wherein The device further comprises at least one supporting mechanism, which is at least arranged at the second end of the platform for clamping an end of the culture bag close to the second end of the platform.

3. The automated cell culture device according to claim 2, wherein: The at least one supporting mechanism includes at least two clamping members, and the two clamping members are respectively arranged on opposite sides of the body to clamp opposite sides of one end of the culture bag close to the second end of the platform.

4. The automated cell culture device according to claim 3, wherein Each of the clamping parts comprises two magnetic blocks, wherein one of the magnetic blocks is fixed on the platform, and the other magnetic block is detachably attracted to the magnetic block fixed on the platform so as to clamp one corner of the culture bag between the two magnetic blocks.

5. The automated cell culture device according to claim 1, wherein The bag clamping component comprises two cross bars, the two cross bars are respectively perpendicular to the sliding direction, the two cross bars are spaced apart from each other, and the distances between the two cross bars and the platform are different.

6. The automated cell culture device according to claim 5, wherein: The platform has a slide rail portion on each of the opposite sides. The bag clamping part further includes two connecting parts and two sliding parts. The opposite ends of each cross bar are fixed to the two connecting parts. The two connecting parts are detachably arranged on the two sliding parts. The two sliding parts are slidably arranged on the two slide rail portions.

7. The automated cell culture device according to claim 6, wherein: The invention further comprises a moving drive mechanism, which is arranged in the accommodating space of the main body and comprises a belt set and two magnetic parts. The two magnetic parts are arranged on the belt set and can be driven by the belt set to move along the sliding direction. Each of the sliding parts is respectively provided with a magnetic part. The magnetic parts of the two sliding parts are respectively magnetically attracted to the two magnetic parts, so that the belt set can drive the two sliding parts to move along the sliding direction.

8. The automated cell culture device according to claim 1, wherein: The invention further comprises a pushing mechanism which is arranged in the accommodation space of the main body and located at the first end of the main body. The pushing mechanism can move up and down to reciprocally lift the end of the culture bag close to the first end upward and reset it downward.

9. The automated cell culture device according to claim 8, wherein The pushing mechanism includes a rotating device, an eccentric wheel and a pushing plate. The eccentric wheel is eccentrically arranged on the rotating device and rotates eccentrically with the rotating device. The peripheral edge of the eccentric wheel is used to abut the pushing plate, and the pushing plate is used to abut the culture bag. When the eccentric wheel rotates eccentrically, the pushing plate is reciprocally pushed upward and reset downward by the eccentric wheel.

10. The automated cell culture device according to claim 1, wherein The invention further comprises a breathable member which is arranged on the platform and has a concave-convex structure on its surface. The breathable member is sandwiched between the platform and the culture bag so as to allow the bottom surface of the culture bag to be breathable.