RESIDUAL URINE NEUTRALIZING COMPOSITIONS AND COATINGS

Abstract

A liquid composition that forms a residual hydrophobic coating on a substrate post-application that neutralizes urine odor and / or degrades urine including water, microbial spore(s) and an enzyme dispersed within the water present in an effective amount to reduce and / or eliminate urine odor and / or degrade urine on the substrate post-application; and a film forming composition dispersed within the water present in an effective amount to form a clear hydrophobic film on the substrate post-application. A residual hydrophobic coating formed on a substrate that neutralizes urine odor and / or degrades urine for a predetermined period of time.

Description

l RESIDUALURINENEUTRALIZHIG COD / [POSITIONSAND COATHIGS TECIHIICAL FIELD

[0001] The present invention relates generally to the field of liquid compositions and coatings formed therefrom, and more particularly, to liquid compositions and coatings that neutralizes urine odor and / or degrades urine. BACKGROUND

[0002] Public spaces, such as public bathrooms and high touch surfaces, are hard to maintain often-times associated with extraneous urines splashes, lingering malodors, etc. These public spaces require constant cleaning and disinfection in an attempt to maintain a cleanly appearance.

[0003] Many conventional products either mask or temporarily eliminate various smells in bathrooms to keep these spaces smelling fresh for a short amount oftime. However, the malodors quickly return requiring frequent and repeated cleaning. The frequent reapplication of these products disadvantageously requires frequent, excessive use ofcleaning products coupled with frequent manual labor to reapply these cleaning products, thus resulting in a very costly, inefficient endeavor. SUlVVIARY

[0004] Inview ofthe above-mentioned problems associated with lingering odors, extraneous urines splashes, etc., there is a need to provide compositions and coatings (residual coatings) that maintain bathroom cleanliness and bathroom surface cleanliness as well as providing a fresh smell and / or reduced odor within bathroom spaces for prolonged periods of time especially when compared to conventional cleaning products. The coatings disclosed herein are further advantageously capable ofwithstanding routine cleaning practices while concurrently maintaining odor neutralizing and / or odor degrading properties, which includes but is not limited to neutralizing urine odor and / or degrading urine on, for example, bathroom surfaces.

[0005] In certain aspects, disclosed is a liquid composition that forms a residual hydrophobic coating on a substrate post-application that neutralizes urine odor and / or degrades urinewhen the residual hydrophobic coating is contacted with urine comprising: (a) water at a concentration ofup to 80wt% ofthe liquid composition, (b) microbial spore(s) and an enzyme dispersed, preferably homogeneously dispersed, within the water present in an effective amount to reduce and / or eliminate urine odor and / or degrade urine on the substrate post-application, and (c) a film forming composition dispersed, preferably homogeneously dispersed, within the water present in an effective amount to form a clear hydrophobic film on the substrate post- application.

[0006] In certain aspects, the liquid composition has pH ranging from 5.0 to 9.8 as well as a viscosity ranging from 0 cPs to 100 cPs.

[0007] In certain aspects, the liquid composition further comprising a coalescing agent present in an effective amount to lower Tg ofthe film forming composition to facilitate film formation post-application ofthe liquid composition on the substrate at ambient temperature.

[0008] In certain aspects, the coalescing agent is an ester alcohol, a glycol ester, or a combination thereof.

[0009] In certain aspects, the coalescing agent is 2,2,4-Trimethyl-1,3-pentanediol-iso- butyrate.

[0010] In certain aspects, the coalescing agent has a concentration ranging from 0.15 wt% to 1.0 wt%, more preferably from 0.25 wt% to 0.95 wt% ofan overall concentration ofthe liquid composition.

[0011] In certain aspects, the film forming composition is configured to adhere to the substrate and / or is configured to adhere the microbial spore(s) and the enzyme to the substrate.

[0012] In certain aspects, the film forming composition is anionic, non-ionic, or a combination thereofand / or wherein the film forming composition comprises 8wt% to 40 wt%, more preferably 10wt% to 35 wt% ofthe overall concentration ofthe liquid composition.

[0013] In certain aspects, the film forming composition comprises an anionic or non- ionic acrylate, an anionic or non-ionic silicon compound, or a combination thereof.

[0014] In certain aspects, the anionic or non-ionic acrylate is an acrylic homopolymer.

[0015] In certain aspects, the anionic or non-ionic acrylate is present in the liquid composition at a concentration ranging from 7.5 wt% to 37 wt%, more preferably 9.75 wt% to 33.5 wt% ofthe overall concentration ofthe liquid composition.

[0016] In certain aspects, the film forming composition comprises the anionic acrylate.

[0017] In certain aspects, the anionic acrylate is an anionic acrylic homopolymer.

[0018] In certain aspects, the anionic acrylate is present in the liquid composition at a concentration ranging from 7.5 wt% to 37 wt%, more preferably 9.75 wt% to 33.5 wt% ofthe overall concentration ofthe liquid composition.

[0019] In certain aspects, the film forming composition comprises an anionic or non- ionic silicon compound.

[0020] In certain aspects, the anionic or non-ionic silicon compound is an organosilicon compound.

[0021] In certain aspects, the organosilicon compound is a non-ionic organosilicon compound.

[0022] In certain aspects, the anionic or non-ionic silicon compound is present in the liquid composition at a concentration ranging from 0.1 wt% to 3 wt%, more preferably 0.125 wt% to 2wt% ofthe overall concentration ofthe liquid composition.

[0023] In certain aspects, the film forming composition comprises the anionic or non- ionic acrylate and the anionic or non-ionic silicon compound present in the liquid composition at ratio of 5:1 to 8: 1, more preferably 4:1 to 7:1 relative to one another.

[0024] In certain aspects, the film forming composition comprises the anionic acrylate and the anionic silicon compound present in the liquid composition at ratio of 5:1 to 8: 1, more preferably 4:1 to 7:1 relative to one another.

[0025] In certain aspects, the film forming composition comprises the anionic acrylate and the non-ionic silicon compound present in the liquid composition at ratio of 5:1 to 8: 1, more preferably 4:1 to 7:1 relative to one another.

[0026] In certain aspects, the film forming composition comprises the non-ionic acrylate and the non-ionic silicon compound present in the liquid composition at ratio of 5:1 to 8: 1, more preferably 4:1 to 7:1 relative to one another.

[0027] In certain aspects, the microbial spore(s) are gram positive bacterial spores and / or non-pathogenic and / or are Bacillus spores and / or are Bacillus subtilis spores that degrade urine.

[0028] In certain aspects, the microbial spore(s) are present at a concentration of 2.5 x 107 to 7.5 x 107, more preferably 3 x 107 to 6.75 x 107 cfu / mL in the liquid composition.

[0029] In certain aspects, the enzyme is an anionic enzyme.

[0030] In certain aspects, the enzyme is a lipase present at an effective amount to degrade urine and / or lipids within urine.

[0031] In certain aspects, the enzyme has a concentration ranging from 0.075 wt% to 0.25 wt%, more preferably 0.1 wt% to 0.22wt% ofthe overall concentration ofthe liquid composition.

[0032] In certain aspects, the microbial spore(s) and the enzyme comprise 10wt% to 28 wt% ofan overall weight of liquid composition.

[0033] Also disclosed is a residual hydrophobic coating formed on a substrate post- application ofthe liquid composition to the substrate, Wherein the residual hydrophobic coating neutralizes urine odor and / or degrades urine for a predetermined period oftime.

[0034] Also disclosed is a residual hydrophobic coating that neutralizes urine odor and / or degrades urine comprising: (a) microbial spore(s) and an enzyme present in an effective amount to reduce and / or eliminate urine odor and / or degrade urinewhen the residual hydrophobic coating is contacted with urine, and a clear film having the microbial spore(s) and the enzyme composition dispersed, preferably homogeneously dispersed, therein.

[0035] In certain aspects, the residual hydrophobic coating is covalently bound, electrostatically bound, or adhered by physical interaction(s) to the substrate.

[0036] In certain aspects, the clear film is anionic, non-ionic, or a combination thereof.

[0037] In certain aspects, the clear film comprises 15.0wt% to 60 wt%, more preferably 15.5 wt% to 55 wt% ofan overall concentration ofthe residual hydrophobic coating.

[0038] In certain aspects, the clear film comprises an anionic or non-ionic acrylate, an anionic or non-ionic silicon compound, or a combination thereof.

[0039] In certain aspects, the anionic or non-ionic acrylate is an acrylic homopolymer.

[0040] In certain aspects, the anionic or non-ionic acrylate is at a concentration ranging from 12.5 wt% to 55.0wt% ofthe overall concentration ofthe residual hydrophobic coating.

[0041] In certain aspects, the clear film comprises the anionic acrylate.

[0042] In certain aspects, the anionic acrylate is an anionic acrylic homopolymer.

[0043] In certain aspects, the anionic acrylate is present at a concentration ranging from 12.5 wt% to 55.0wt% ofthe overall concentration ofthe residual hydrophobic coating.

[0044] In certain aspects, the clear film composition comprises an anionic or non-ionic silicon compound.

[0045] In certain aspects, the anionic or non-ionic silicon compound is an organosilicon compound.

[0046] In certain aspects, the organosilicon compound is a non-ionic organosilicon compound.

[0047] In certain aspects, the anionic or non-ionic silicon compound is present at a concentration ranging from 0.4wt% to 5.0wt% ofthe overall concentration ofthe residual hydrophobic coating.

[0048] In certain aspects, the clear film comprises the anionic or non-ionic acrylate and the anionic or non-ionic silicon compound present in the residual hydrophobic coating at ratio of 5:1 to 8: 1, more preferably 4:1 to 7:1 relative to one another.

[0049] In certain aspects, the clear film comprises the anionic acrylate and the anionic silicon compound present in the residual hydrophobic coating at ratio of 5:1 to 8: 1, more preferably 4:1 to 7:1 relative to one another.

[0050] In certain aspects, the clear film comprises the anionic acrylate and the non-ionic silicon compound present in the residual hydrophobic coating at ratio of 5:1 to 8: 1, more preferably 4:1 to 7:1 relative to one another.

[0051] In certain aspects, the clear film comprises the non-ionic acrylate and the non- ionic silicon compound present in the residual hydrophobic coating at ratio of 5:1 to 8: 1, more preferably 4:1 to 7:1 relative to one another.

[0052] In certain aspects, the microbial spore(s) are Bacillus spores that degrade urine.

[0053] In certain aspects, the microbial spore(s) are present at a concentration of 2.5 x 107 to 7.5 x 107, more preferably 3 x 107 to 6.75 x 107 cfu / mL in the residual hydrophobic coating.

[0054] In certain aspects, the enzyme is an anionic enzyme.

[0055] In certain aspects, the enzyme is a lipase present at an effective amount to degrade urine and / or lipids within urine.

[0056] In certain aspects, the enzyme has a concentration ranging from 0.5 wt% to 5.0wt% ofthe overall concentration ofthe residual hydrophobic coating.

[0057] In certain aspects, the enzyme has a concentration more preferably ranging from 0.5 wt% to 4.5 wt% ofthe overall concentration ofthe residual hydrophobic coating.

[0058] In certain aspects, the microbial spore(s) and the enzyme comprise 42.0wt% to 83 wt% ofan overall weight ofthe residual hydrophobic coating.

[0059] Also disclosed is an article comprising: (a) a substrate; and (b) a residual hydrophobic coating that neutralizes urine odor and / or degrades urine on the substrate.

[0060] In certain aspects, wherein the substrate is a rigid substrate, a non-porous substrate, or a combination thereof.

[0061] In certain aspects, wherein the substrate is glass, ceramic, metal, plastic, cementitious fillers (e.g., grout), elastomer(s) (e.g., elastomeric caulk), or any combination thereof.

[0062] Also disclosed is a method ofmaking the liquid composition comprising dispersing microbial spore(s), enzyme, and a film forming composition in water and optionally dispersing a coalescing agent therein thereby forming the liquid composition.

[0063] Also disclosed is a method comprising applying the liquid composition to a substrate and forming a residual hydrophobic coating on the substrate post-application ofthe liquid composition, Wherein the residual hydrophobic coating neutralizes urine odor and / or degrades urinewhen contacted with urine.

[0064] Embodiments ofthe invention can include one or more or any combination ofthe above features and configurations.

[0065] Additional features, aspects and advantages ofthe invention will be set forth in the detailed description which follows, and in part will be readily apparent to those skilled in the art from that description or recognized by practicing the invention as described herein. It is to be understood that both the foregoing general description and the following detailed description present various embodiments ofthe invention and are intended to provide an overview or framework for understanding the nature and character ofthe invention as it is claimed. The accompanying drawings are included to provide a further understanding ofthe invention and are incorporated in and constitute a part ofthis specification. BRIEFDESCRIPTIONOFTHEDRAWEIGS

[0066] These and other features, aspects and advantages ofthe present invention are better understoodwhen the following detailed description ofthe invention is read with reference to the accompanying drawings, in which:

[0067] FIG. 1 is a photograph of a porcelain substrate soiled with urine.

[0068] FIG. 2 is a photograph of a porcelain substrate soiled with urine twenty-four (24) hours post-treatment with an exemplary liquid composition (post-drying / post-curing thereon). DETAILEDDESCRIPTION

[0069] The present invention will now be described more fully hereinafter with reference to the accompanying drawings in which exemplary embodiments ofthe invention are shown. However, the invention may be embodied in many different forms and should not be construed as limited to the representative embodiments set forth herein. The exemplary embodiments are provided so that this disclosure will be both thorough and complete, and will fully convey the scope ofthe invention and enable one ofordinary skill in the art to make, use and practice the invention. Like reference numbers refer to like elements throughout the various drawings.

[0070] Further, the term or as used in this disclosure and the appended claims is intended to mean an inclusive or rather than an exclusive or. That is, unless specified otherwise, or clear from the context, the phrase X employsA orB is intended to mean any of the natural inclusive permutations. That is, the phraseX employsA orB is satisfied by any of the following instances: X employs A,X employs B; orX employs bothA and B. In addition, the articles a and an as used in this application and the appended claims should generally be construed to mean one or more unless specified otherwise or clear from the context to be directed to a singular form. Throughout the specification and claims, the following terms take at least the meanings explicitly associated herein, unless the context dictates otherwise. The meanings identified below do not necessarily limit the terms, but merely provide illustrative examples for the terms. The meaning ofa, an, and the may include plural references, and the meaning of in may include in, at, and / or on, unless the context clearly indicates otherwise. The phrase in one embodiment, as used herein does not necessarily refer to the same embodiment, although it may.

[0071] Concentrations, amounts, and other numerical data may be expressed or presented herein in a range format. It is to be understood that such a range format is used merely for convenience and brevity and thus should be interpreted flexibly to include not only the numerical values explicitly recited as the limits ofthe range, but also to include all the individual numerical values or sub-ranges encompassed within the ranges as ifeach numerical value and sub-range is explicitly recited. As an illustration, a numerical range ofabout 1 to 5 should be interpreted to include not only the explicitly recited values ofabout 1 to about 5, but also include individual values and sub-ranges within the indicated range. Thus, included in this numerical range are individual values such as 2, 3, and 4 and sub-ranges such as from 1-3, from 2-4, and from 3-5, etc. as well as 1, 2, 3, 4, and 5, individually. The same principle applies to ranges reciting only one numerical value as a minimum or a maximum. Furthermore, such an interpretation should apply regardless ofthe breadth ofthe range or the characteristics being described. Liguid Composition ThatForms The Residual Hydrophobic Coating OnA Substrate Post- Application

[0072] Disclosed is a liquid composition that forms a residual hydrophobic coating on a substrate post-application that neutralizes urine odor (human urine odor) and / or degrades urine (human urine) when the residual hydrophobic coating is contacted with urine (e.g., human urine). The liquid compositions includes (a) water at a concentration ofup to 80wt% ofthe liquid composition, (b) microbial spore(s) and an enzyme dispersed, preferably homogeneously dispersed, within the water present in an effective amount to reduce and / or eliminate urine odor and / or degrade urine on the substrate post-application, and (c) a film forming composition dispersed, preferably homogeneously dispersed, within the water present in an effective amount to form a clear hydrophobic film on the substrate post-application. In certain aspects, water is included within the liquid composition at a concentration of45 wt% to 80 wt%, more preferably 50wt% to 80 wt%, even more preferably 55 wt% to 80wt% ofthe overall concentration ofthe liquid composition, in which any endpoint within these ranges can serve as endpoints for any additional sub-range falling therein.

[0073] The liquid composition has apH ranging from 5.0 to 9.8, more preferably 7.0 to 9.8 in which any endpoint within these ranges can serve as endpoints for any additional sub- range falling therein as well as a viscosity of 0 cPs to 100 cPs, more preferably of 0 cPs to 50 cPs at ambient temperature (0 °C to 30 °C) in which any endpoint within these ranges can serve as endpoints for any additional sub-range falling therein so that the liquid composition may be adequately and / or evenly (e.g., uniformly and / or homogeneously) applied to a substrate and / or so that, for example, the microbial spore(s) and / or enzyme maintains desired activity / operability both while in storage (e.g., shelf-life) and / or during and / or post-application to the substrate. As opposed to point-of-use compositions that are used immediately after production on-site, it should be understood that the disclosed liquid compositions should be shelf-stable and / or exhibit desired storage stability for a predetermined period oftime, which includes six (6) months to five (5) years in which the microbial spore(s) and / or enzymes included within the liquid composition maintain, for example, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least99% ofthe properties and / or efficacy disclosed herein during this predetermined period oftime, thus avoiding the need ofimmediate application immediately after the liquid composition is manufactured / made.

[0074] As alluded to above, the liquid composition disclosed herein preferably includes a blend / mixture ofmicrobial spore(s) and an enzyme dispersed, preferably homogeneously dispersed, within the water at an effective amount to reduce and / or eliminate urine odor and / or degrade urine on a substrate post-application (and, for example, post-cure / post-drying). The microbial spore(s) and / or enzymes degrade urine and / or neutralize the smells associated with urine (human urine) and / or the by-products thereof (e.g., ammonia and / or bicarbonate) and / or additional substances (e.g., lipids and / or proteins) found within and / or excreted with urine.

[0075] The microbial spore(s) disclosed herein are preferably gram-positive bacterial spore and / or non-pathogenic microbial spores, more preferably Bacillus spores and even more preferably Bacillus subtilis spores that degrade urine. In certain aspects, the non-pathogenic microbial spores, more preferably Bacillus spores, more preferably Bacillus subtilis are non- genetically modified spores while in other aspects the Bacillus spores are genetically modified with transgenes that advantageously degrade urine and / or neutralize the smells associated with urine and / or the by-products (ammonia and / or bicarbonate) thereofand / or additional substances (e.g., lipids and / or proteins) found within and / or excreted with urine. The microbial spore(s), and more particularly, Bacillus spores, are present at a concentration of2.5 x 107 to 7.5 x 107, more preferably 3 x 107 to 6.75 x 107 cfu / mL in which any endpoint within these ranges can serve as endpoints for any additional sub-range falling therein in the liquid composition to advantageously achieve the desired urine degradation and / or neutralization and / or by-product degradation / neutralization (ammonia and / or bicarbonate) and / or degradation / neutralization of additional substances (e.g., lipids and / or proteins) post-application and post-drying / post-cure of the liquid composition to the substrate for a desired period oftime.

[0076] The enzyme included within the liquid composition preferably degrades lipids (fats) and / or proteins typically found in urine (human urine) and is included within the liquid composition at effective amounts / concentrations to do so. In certain aspects, the enzyme is an anionic enzyme in order to interact with other components in the liquid composition and to maintain efficacy both within the liquid composition and post-application ofthe liquid composition onto a substrate. The enzyme is preferably a lipase (more preferably a bacterial lipase or a lipase produced by (and / or isolated from) bacterial fermentation processes), and more preferably an anionic lipase and / or negatively charged lipase that degrades lipids (fats) and / or proteins found in urine (human urine). In certain aspects, the enzyme has a concentration ranging from 0.075 wt% to 0.25 wt%, more preferably from 0.1 wt% to 0.22wt% ofthe overall concentration ofthe liquid composition in which any endpoint within these ranges can serve as endpoints for any additional sub-range falling therein to degrade lipids (fats) and / or proteins that may be found in urine (human urine).

[0077] In certain aspects, the microbial spore(s) and the enzyme comprise 10wt% to 28 wt%, more preferably 12.5 wt% to 25 wt% ofan overall weight of liquid composition in which any endpoint within these ranges can serve as endpoints for any additional sub-range falling therein.

[0078] The film forming composition included within the liquid composition is preferably configured to adhere to the substrate and / or to adhere the microbial spore(s) and the enzyme to the substrate. Moreover, the film forming composition has hydrophobic properties as well as hardness properties such that the film forming composition preferably forms a hydrophobic coating having sufficient hardness and durability post-application and / or post- cure / post-drying ofthe disclosed liquid composition onto a substrate. In certain aspects, the film forming composition is anionic, non-ionic, or a combination thereofand / or wherein the film forming composition comprises 8 wt% to 40 wt%, more preferably 10wt% to 35 wt% ofthe overall concentration ofthe liquid composition in which any endpoint within these ranges can serve as endpoints for any additional sub-range falling therein. In certain aspects and to achieve the desired properties mentioned above, the film forming composition may be a two-part film forming composition that includes, for example, an anionic or non-ionic acrylate, an anionic or non-ionic silicon compound, or combinations thereof. In certain aspects, the two-part film forming composition includes an anionic or non-ionic acrylate and an anionic or non-ionic silicon compound, and in this aspect, the anionic or non-ionic acrylate is present at greater concentrations within the liquid composition than the anionic or non-ionic silicon compound.

[0079] The anionic or non-ionic acrylate is preferably configured to act as a binder system that, post-application ofthe liquid composition to a substrate, adheres (e.g., homogeneously and / or evenly and / or uniformly adheres) to the substrate and / or that adheres the microbial spore(s) and the enzyme to the substrate. In certain aspects, the anionic or non-ionic acrylate is an acrylic homopolymer configured to act as a binder system that adheres (e.g., homogeneously and / or evenly and / or uniformly adheres) to the substrate and / or that adheres the microbial spore(s) and the enzyme to the substrate. The anionic or non-ionic acrylate, more preferably an anionic acrylate, and even more preferably an acrylic homopolymer (anionic acrylic homopolymer) is present in the liquid composition at a concentration ranging from 7.5 wt% to 37 wt%, more preferably 9.75 wt% to 33.5 wt% ofthe overall concentration ofthe liquid composition in which any endpoint within these ranges can serve as endpoints for any additional sub-range falling therein. In certain aspects, concentrations of less than 7.5 wt% anionic or non- ionic acrylate ofthe overall concentration ofthe liquid composition are not used because film formation as well as adherence ofthe microbial spore(s) / enzyme is adversely affectedwhen applied to the substrate resulting in an inferior, undesired coating / film. Conversely, if37wt% of anionic or non-ionic acrylate ofthe overall concentration ofthe liquid composition is exceeded, film formation is negatively affected as well because the film becomes too glossy (aesthetically displeasing) and thick, which affects microbial spore(s) / enzyme activity and dispersibility within the film as well as film hydrophobicity provided / imparted by the anionic or non-ionic silicon compound within the film forming composition. In certain aspects, cationic acrylates are not included within the liquid compositions as they may adversely impact enzyme activity and / or microbial spore efficacy.

[0080] The film forming composition and the film formed therefrom are preferably hydrophobic. To achieve the desired hydrophobicity, the film forming composition further includes a silicon compound, more preferably an anionic or non-ionic silicon compound therein. In certain aspects, the anionic or non-ionic silicon compound is an organosilicon compound. In certain aspects, the organosilicon compound is a non-ionic organosilicon compound. In certain aspects, the anionic or non-ionic silicon compound, more preferably an anionic or non-ionic organosilicon compound, even more preferably a non-ionic organosilicon compound is present in the liquid composition at a concentration ranging from 0.1 wt% to 3 wt%, more preferably 0.125 wt% to 2wt% ofthe overall concentration ofthe liquid composition in which any endpoint within these ranges can serve as endpoints for any additional sub-range falling therein. [0081 In certain aspects, the film forming composition comprises the anionic or non- ionic acrylate and the anionic or non-ionic silicon compound present in the liquid composition at ratio of 5:1 to 8: 1, more preferably 4:1 to 7:1 relative to one another in which any endpoint within these ratio ranges can serve as endpoints for any additional sub-range falling therein. In certain aspects, the lm forming composition comprises the anionic acrylate and the anionic silicon compound present in the liquid composition at ratio of 5:1 to 8: 1, more preferably 4:1 to 7:1 relative to one another in which any endpoint within these ratio ranges can serve as endpoints for any additional sub-range falling therein. In certain aspects, the lm forming composition comprises the anionic acrylate and the non-ionic silicon compound present in the liquid composition at ratio of 5:1 to 8:1, more preferably 4:1 to 7:1 relative to one another in which any endpoint within these ratio ranges can serve as endpoints for any additional sub-range falling therein. In certain aspects, the lm forming composition comprises the non-ionic acrylate and the non-ionic silicon compound present in the liquid composition at ratio of 5:1 to 8:1, more preferably 4:1 to 7:1 relative to one another in which any endpoint within these ratio ranges can serve as endpoints for any additional sub-range falling therein. In certain aspects, when the anionic or non-ionic acrylate ratio falls below 5:1 to anionic or non-ionic silicon compound, film formation as well as adherence ofthe microbial spore(s) / enzyme is adversely affectedwhen applied to the substrate. Conversely, when the anionic or non-ionic acrylate ratio exceeds 8:1 to anionic or non-ionic silicon compound film, film formation is negatively affected as well because the lm becomes too glossy (aesthetically displeasing) and thick, which affects microbial spore(s) / enzyme activity and dispersibility within the film as well as film hydrophobicity provided / imparted by the anionic or non-ionic silicon compound within the film forming composition.

[0082] Ifthe upper concentration endpoints and / or upper ratio endpoints ofthe anionic or non-ionic acrylate disclosed herein are exceeded relative to the anionic or non-ionic silicon compound in the liquid composition that forms a residual hydrophobic coating, durability and / or hydrophobicity ofthe resulting coating (post-application and / or post-drying / curing ofthe liquid composition) will be compromised, thus resulting in a sub-optimal, short-lived coating having sub-optimal hydrophobic properties.

[0083] In certain aspects, the film forming composition(s) disclosed herein have difficulty forming films and / or uniform and / or homogeneous films at ambient temperatures often requiring additional heat and / or a heating step to form films. In view ofthe need to form a film / coating post-application ofthe disclosed liquid composition on a substrate at ambient temperature(s), this problem was addressed, and coalescing agent(s) were identified that advantageously lowered the glass transition temperature(s) (Tg) ofthe film forming compositions and / or the disclosed liquid composition thereby allowing for film formation / coating formation at ambient temperature. Thus, in certain aspects, the liquid composition further includes a coalescing agent present in an effective amount to lower Tg ofthe film forming composition to facilitate film formation post-application ofthe liquid composition on the substrate at ambient temperature.

[0084] In certain aspects, the coalescing agent lowers the Tg ofthe liquid compositions and / or ofthe film forming agents by 10 °C to 25 °C, more preferably from 15 °C to 20 °Cwhen compared to the initial Tg ofthe liquid composition and / or ofthe film forming agents (without the coalescing agent therein), thereby allowing the liquid compositions to be applied and form films / coatings disclosed herein at ambient temperatures ranging from 20 °C to 25 °C. The coalescing agent is an ester alcohol, a glycol ester, or a combination thereof, and in certain aspects, the coalescing agent is 2,2,4-Trimethyl-1,3-pentanediol-iso-butyrate. The coalescing agent has a concentration ranging from 0.15 wt% to 1.0 wt%, more preferably from 0.25 wt% to 0.95 wt% ofan overall concentration ofthe liquid composition in which any endpoint within these ranges can serve as endpoints for any additional sub-range falling therein. In certain aspects, concentrations of less than 0.15 wt% ofthe coalescing agent ofthe overall concentration ofthe liquid composition are not used because film formation is adversely affected such that film formation does not and / or does not adequately occur at ambient temperature. Conversely, if 1.0wt% ofthe coalescing agent ofthe overall concentration ofthe liquid composition is exceeded, film formation is negatively affected as well because the film is plasticized becoming too thick and / or opaque thereby having an aesthetically displeasing appearance, which further affects microbial spore(s) / enzyme activity and dispersibility within the film.

[0085] In certain aspects, additional fragrances and odor counteractants may be included in the liquid compositions at concentrations ofup to 6.5 wt% ofan overall weight of liquid composition in which any endpoint within these ranges can serve as endpoints for any additional l4 sub-range falling therein. These fragrances may include, but are not limited to, one or more aromatic compounds. These odor counteractants may include, but are not limited to, benzaldehyde, bourgeonal, cinnamaldehyde, hexyl cinnamaldehyde, citronellal, hydroxy citronella, citral, cuminaldehyde, decanal, eugenol, geraniol, heptanal, cis-3-hexen-1-ol, hexanal, (x-ionone, B-ionone, y-ionone, lyral, nonanaldehyde, octanaldehyde, valeraldehyde, perillaldehyde, piperanal, vanillin, para tert-amyl cyclohexanone, ortho tert-butyl cyclohexanol, 3-cyclohexene-1-carboxaldehyde-4-(4-hydroxy-4-methylpentyl), a-methyl-4-(1-methylethyl) benzenepropanal, para tert-butyl-(x-methyldihydrocinnamic aldehyde, and 4-tert-butyl cyclohexanol.

[0086] Also disclosed herein is an application method that includes (a) applying the liquid composition(s) disclosed herein to a substrate / surface disclosed herein and (b) forming a residual hydrophobic coating on the substrate post-application ofthe liquid composition. The residual hydrophobic coating is formed by drying and / or curing the liquid composition on the substrate, preferably at ambient temperature. Once the residual hydrophobic is formed on the substrate, the residual hydrophobic coating neutralizes urine odor and / or degrades urinewhen contacted with urine for prolonged periods oftime. Residual Hydrophobic Coating That Neutralizes Urine OdorAnd / Or Degrades Urine

[0087] Also disclosed is a residual hydrophobic coating that neutralizes urine odor (human urine odor) and / or degrades urine (human urine) comprising: (a) microbial spore(s) and an enzyme present in an effective amount to reduce and / or eliminate urine odor and / or degrade urinewhen the residual hydrophobic coating is contacted with urine, and (b) a clear film having the microbial spore(s) and the enzyme composition dispersed, preferably homogeneously dispersed, therein. The residual hydrophobic coating is formed on the substrate post-application and drying / curing ofthe liquid composition on the substrate and includes less than 36wt% water, more preferably less than 30wt% water ofthe overall concentration ofthe residual hydrophobic coating post-drying / post-cure. In certain aspects, the substrate is a non- functionalized substrate, an electrostatically neutral substrate, a positively charged substrate, or a combination thereof in which the coating is physically bonded / bound to the substrate byVan der Waals forces(s) and / or by hydrogen bonding and / or is covalently bound to the substrate and has an overall thickness (preferably uniform or substantially uniform thickness) ranging from 0.5 um to 250 um, more preferably 0.5 um to 75 um, more preferably 0.5 um to 25 um with any additional endpoint falling within this broad range serving as endpoints for any additional sub- range thicknesses occurring therein. In preferred embodiments, the residual hydrophobic coating has a uniform (and / or substantially uniform) and / or even thickness (and / or substantially even thickness) (e.g., when viewed in cross-section) and / or is uniformly applied along a surface (outer surface) ofthe substrate along with the microbial spore(s) and / or enzyme dispersed, preferably homogeneously dispersed, within and / or throughout the residual hydrophobic coating.

[0088] The residual hydrophobic coating preferably exhibits hydrophobic and / or water repellant properties such that the coating remains substantially intact and operable over prolonged periods oftime ranging from 1 day to 14 days post-application / post-formation and even after frequent and / or repeated applications of conventional cleaning products thereon. Conventional cleaning products include, but are not limited to, water-based cleaners such as Formula 409® that include quaternary ammonium actives / biocidals.

[0089] Post-application and post-drying / curing ofthe liquid composition, the film forming composition ofthe liquid composition forms a clear film in the residual hydrophobic coating, in which the clearlm is anionic, non-ionic, or a combination thereof. The clear film comprises 12.0wt% to 60.0wt% ofan overall concentration ofthe residual hydrophobic coating in which any endpoint within these ranges can serve as endpoints for any additional sub-range falling therein.

[0090] To obtain desired coating durability and / or hydrophobicity, in certain aspects, the clear film comprises a two-part or two-component system (film forming composition within the above-mentioned liquid composition) comprising an anionic or non-ionic acrylate, an anionic or non-ionic silicon compound, or a combination thereof. The anionic or non-ionic acrylate is an acrylic homopolymer, more preferably an anionic acrylic homopolymer. The anionic or non- ionic acrylate, more preferably an anionic or non-ionic acrylic homopolymer, even more preferably an anionic acrylic homopolymer is at a concentration ranging from 12.5 wt% to 55.0 wt% ofthe overall concentration ofthe residual hydrophobic coating in which any endpoint within these ranges can serve as endpoints for any additional sub-range falling therein. The anionic or non-ionic acrylate, more preferably an anionic or non-ionic acrylic homopolymer, even more preferably an anionic acrylic homopolymer is a binder system that adheres (e.g., homogeneously and / or evenly and / or uniformly adheres) to the substrate and / or that adheres the microbial spore(s) and the enzyme disclosed herein to the substrate. In certain aspects, concentrations of less than 12.5 wt% anionic or non-ionic acrylate, more preferably an anionic or non-ionic acrylic homopolymer, even more preferably an anionic acrylic homopolymer ofthe overall concentration ofthe residual hydrophobic coating are not used because film formation as well as adherence ofthe microbial spore(s) / enzyme is adversely affected (non-uniform and heterogeneous film formation and / or non-uniform and / or heterogeneous dispersion ofthe microbial spore(s) and / or enzyme therein). Conversely, if 55.0wt% ofanionic or non-ionic acrylate, more preferably an anionic or non-ionic acrylic homopolymer, even more preferably an anionic acrylic homopolymer ifthe overall concentration ofthe residual hydrophobic coating is exceeded, film formation is negatively affected as well because the resulting film is too glossy (aesthetically displeasing) and thick, which affects microbial spore(s) / enzyme activity and dispersibility within the lm as well as film hydrophobicity provided / imparted by the anionic or non-ionic silicon compound.

[0091] To further obtain desired coating durability and / or hydrophobicity, the clear film composition (as well as the coating) and more particularly two-part or two-component system disclosed above further includes an anionic or non-ionic silicon compound. In certain aspects, the anionic or non-ionic silicon compound is an organosilicon compound and even more preferably is a non-ionic organosilicon compound. The anionic or non-ionic silicon compound, more preferably an organosilicon compound, and even more preferably the non-ionic organo- silicon compound is present at a concentration ranging from 0.4wt% to 5.0 wt%, more preferably 0.4wt% to 4.6wt% ofthe overall concentration ofthe residual hydrophobic coating in which any endpoint within these ranges can serve as endpoints for any additional sub-range falling therein.

[0092] In certain aspects, the residual hydrophobic coating includes the anionic or non- ionic acrylate and the anionic or non-ionic silicon compound at ratio of 5:1 to 8: 1, more preferably 4:1 to 7:1 relative to one another in which any endpoint within these ratio ranges can serve as endpoints for any additional sub-range falling therein. In certain aspects, the residual hydrophobic coating includes the anionic acrylate and the anionic silicon compound at ratio of 5:1 to 8:1, more preferably 4:1 to 7:1 relative to one another in which any endpoint within these ratio ranges can serve as endpoints for any additional sub-range falling therein. In certain aspects, the residual hydrophobic coating includes the anionic acrylate and the non-ionic silicon compound at ratio of 5:1 to 8:1, more preferably 4:1 to 7:1 relative to one another in which any l7 endpoint within these ratio ranges can serve as endpoints for any additional sub-range falling therein. In certain aspects, the residual hydrophobic coating includes the non-ionic acrylate and the non-ionic silicon compound at ratio of 5:1 to 8: 1, more preferably 4:1 to 7:1 relative to one another in which any endpoint within these ratio ranges can serve as endpoints for any additional sub-range falling therein. In certain aspects, when the anionic or non-ionic acrylate ratio falls below 5:1 to anionic or non-ionic silicon compound, film formation as well as adherence ofthe microbial spore(s) / enzyme is adversely affectedwhen applied to and / or drying / curing on the substrate. Conversely, when the anionic or non-ionic acrylate ratio exceeds 8:1 to anionic or non-ionic silicon compound, film formation is negatively affected as well because the lm becomes too glossy (aesthetically displeasing) and thick, which affects microbial spore(s) / enzyme activity and dispersion as well as film hydrophobicity ofthe hydrophobic residual coating. [0093 As alluded to above, the microbial spore(s) and enzyme are present in the hydrophobic residual coating at an effective amount to reduce and / or eliminate urine odor and / or degrade urinewhen the residual hydrophobic coating is contacted with urine for prolonged time periods, which range from 1 day to 14 days, preferably 1 day to 5 days. In certain aspects, the microbial spore(s) and / or enzyme are dispersed, preferably homogeneously dispersed, throughout the residual hydrophobic coating including being dispersed, preferably homogeneously dispersed, throughout the clear film in the residual hydrophobic coating.

[0094] The microbial spore(s) disclosed herein are preferably gram-positive bacterial spore and / or non-pathogenic microbial spores, more preferably Bacillus spores, and even more preferably Bacillus subtilis spores that degrade urine. In certain aspects, the non-pathogenic microbial spores, more preferably Bacillus spores, more preferably Bacillus subtilis spores are non-genetically modified spores while in other aspects the Bacillus spores are genetically modified with transgenes that advantageously degrade urine and / or neutralize the smells associated with urine and / or the by-products (ammonia and / or bicarbonate) thereofand / or additional substances (e.g., lipids and / or proteins) found within and / or excreted with urine. The microbial spore(s), and more particularly, Bacillus spores, are present in the hydrophobic residual coating at a concentration of2.5 x 107 to 7.5 x 107, more preferably 3 x 107 to 6.75 x 107 cfu / mL in which any endpoint within these ranges can serve as endpoints for any additional sub- range falling therein to advantageously achieve the desired urine degradation and / or neutralization and / or by-product degradation / neutralization (ammonia and / or bicarbonate) and / or degradation / neutralization ofadditional substances (e.g., lipids and / or proteins) for a desired period oftime by the coating.

[0095] The enzyme included within the residual hydrophobic coating preferably degrades lipids (fats) and / or proteins typically found in urine (human urine) and is included within the residual hydrophobic coating at effective amounts / concentrations to do so. In certain aspects, the enzyme is an anionic enzyme because in order to interact with other components in the liquid composition and to maintain efficacy both within the liquid composition and post-application of the liquid composition onto a substrate. The enzyme is preferably a lipase (more preferably a bacterial lipase or a lipase produced by (and / or isolated from) bacterial fermentation processes), and more preferably an anionic lipase and / or negatively charged lipase that degrades lipids (fats) and / or proteins found in urine (human urine). In certain aspects, the enzyme has a concentration ranging from 0.5 wt% to 5.0 wt%, more preferably from 1.0wt% to 4.5 wt%, more preferably 1.25 wt% to 4.0wt% ofthe overall concentration ofthe residual hydrophobic coating in which any endpoint within these ranges can serve as endpoints for any additional sub-range falling therein, to degrade lipids (fats) and / or proteins thatmay be found in urine (human urine).

[0096] In certain aspects, the microbial spore(s) and the enzyme comprise 42.0wt% to 83 wt% ofan overall weight ofresidual hydrophobic coating in which any endpoint within these ranges can serve as endpoints for any additional sub-range falling therein.

[0097] A coalescing agent is further included within the hydrophobic residual coating. During coating formation, the coalescing agent lowers Tg ofthe liquid compositions and / or of the film forming agents disclosed herein thereby advantageously allowing for drying / curing and film formation ofthe hydrophobic residual coating at ambient temperature(s). The coalescing agent is an ester alcohol, a glycol ester, or a combination thereof, and in certain aspects, the coalescing agent is 2,2,4-Trimethyl-1,3-pentanediol-iso-butyrate. The coalescing agent has a concentration ranging from 0.4wt% to 5.0 wt%, preferably from 0.5 wt% to 4.75 wt%, more preferably from 1 wt% to 4.5 wt%, ofan overall concentration ofthe hydrophobic residual coating in which any endpoint within these ranges can serve as endpoints for any additional sub- range falling therein. Coated Articles / Surfaces

[0098] Also disclosed is an article comprising: (a) a substrate; and (b) the residual hydrophobic coating disclosed herein that neutralizes urine odor and / or degrades urine on the substrate. In certain aspects, wherein the substrate is a rigid substrate, a non-porous substrate, or a combination thereof. Moreover, the substrate is glass, ceramic, metal, plastic, cementitious fillers (e.g., grout), elastomer(s) (e.g., elastomeric caulk), or any combination thereof. The substrate includes toilets, sinks, flooring / flooring surfaces, wall and / or backsplash tile(s), and / or any combination thereofmade from any one ofthe aforementioned materials. In certain aspects, the substrate is a non-functionalized substrate, an electrostatically neutral substrate, a positively charged substrate, or a combination thereof in which the coating is physically bonded / bound to the substrate byVan der Waals forces(s) and / or by hydrogen bonding and / or is covalently bound to the substrate and has an overall thickness (preferably uniform or substantially uniform thickness) ranging from 0.5 um to 250 um, more preferably 0.5 um to 75 um, more preferably 0.5 um to 25 pm , with any additional endpoint falling within this broad range serving as endpoints for any additional sub-range thicknesses occurring therein. In preferred embodiments, the residual hydrophobic coating has a uniform (and / or substantially uniform) and / or even thickness (and / or substantially even thickness) (e.g., whenviewed in cross-section) along with the microbial spore(s) and / or enzyme dispersed, preferably homogeneously dispersed, within and / or throughout the residual hydrophobic coating.

[0099] The residual hydrophobic coating ofthe coated article preferably exhibits hydrophobic and / or water repellant properties such that the coating remains substantially intact and operable over prolonged periods oftime ranging from 1 day to 14 days post- application / post-formation and even after frequent and / or repeated applications ofconventional cleaning products thereon. Conventional cleaning products include, but are not limited to, water- based cleaners such as Formula409® that include quatemary ammonium actives / biocidals. WORKHIG EXAMPLES

[0100] Table 1 discloses a liquid composition (i.e., the residual bathroom deodorizer composition as disclosed herein) and ranges ofthe components in the liquid composition pre- application to the substrate.

[0101] The anionic acrylic homopolymer emulsion, sourced from Gellner IndustrialLLC (Ottopol SF-45 Technical Data Sheet, incorporated by reference herein), is included within the liquid composition(s) in the concentrations shown in Table 1. The40% Organo-Modified Silicone Emulsion, sourced fromMomentive (CoatOSilTM Clean Technical Data Sheet (2022) incorporated by reference herein), is included within the liquid composition(s) in the concentrations shown in Table 1. The odor counteractant, sourced from Belle Aire Creations (Ordenone Specification Sheet (2024) incorporated by reference herein), is included within the liquid composition(s) in the concentrations shown in Table 1. The microbial / enzyme blend, sourced from Barentz (J-Zyme® Technical Data Sheet (2021) incorporated by reference herein), is included within the liquid composition(s) in the concentrations shown in Table 1. The ester alcohol sourced from Eastman (Eastman TexanolTMEster Alcohol (CAS No. 25265-77-4) Product Data Sheet (2013) incorporated by reference herein), is included within the liquid composition(s) in the concentrations shown in Table 1. Watermay be further included in the liquid composition in the concentrations disclosed in Table 1. The Fresh Breeze Scent sourced from Belle Aire® Creations (Fresh Breeze Type Specification Sheet #270347 (December 5, 2024) incorporated by reference herein), is included within the liquid composition(s) in the concentrations shown in Table 1. TABLE 1 Anionic Acrylic Homopolymer 10.0-30.0 __ 40% Organo-Modied Silicone 0.5-2.0 __

[0102] Table 2 discloses the Comparative Examples (i.e., abbreviated Comp #1, Comp #2, and Comp #3) and Examples (i.e., abbreviated Ex. #1 and Ex. #2) including the concentrations ofeach component. As shown in Table 2, the Ex. #2 lacks the 2l microbial / enzyme blend (J-Zyme®) when compared to the other Ex. #1 and Comparative Examples Comp #1, Comp #2, and Comp #3. ThePOLYPEARL 10 micron sourced from Coating Products OHZ (POLYPEARLTMME SERIES Technical Data Sheet (2023) incorporated by reference herein), is included within the liquid composition(s) in the concentrations shown in Table 2. TABLE 2 Components of Comp #1 Comp #2 Comp #3 Ex. #1 Ex. #2 Anionic Acrylic 24 24 24 12 24 Homopolymer Emulsion 40% Organo- 0.5 0.5 0.5 1 0.5 Modified Silicone Emulsion POLYPEARL 10 3 -..... Odor 2 2 2 2 2 -..... Microbial / Enzyme 12 3 3 12 -..... nu-mm Fresh Breeze 1.0 1.0 1.0 1.0 1.0 -.....

[0103] The Comparative Examples and Examples disclosed in Table 2 were tested to obtain the DeltaE values as shown in Table 3. The DeltaE values are measured from 0 to 100 and represent the difference betweentwo colors or the amount ofchange a specific color has undergone, thus quantitating the appearance of stains on a surface. Less color difference or change is observedwhen the value is closer to 0. Thus, the presence of stains will cause the value to increase, while the absence or removal of stains will cause the value to decrease. As shown in Table 3, Ex. #1 has the best stain reducing properties with a DeltaE value of 1.3. The Comparative Example Comp #3 has the worst stain reducing properties with a DeltaE value of 4.54.

[0104] When comparing the compositions, as shown in Table 2, both Examples include more water in their composition compared to the Comparative Examples. However, Ex. #1 includes 12% ofthe acrylic emulsion and 12% ofthe microbial / enzyme blend while Ex. #2 includes 24% ofthe acrylic emulsion and0% ofthe microbial / enzyme blend. It appears there is a relationship between the respective amounts ofthe acrylic emulsion and microbial / enzyme blend. This may be due to the hydrophobic nature ofthe acrylic emulsion which allows it to reduce stains without the microbial / enzyme blend. TABLE 3 -

[0105] Ex. #1 and Comparative Example Comp #3 were applied to the substrate (i.e., subway tile / porcelain tile). These substrates, along with an untreated substrate, were tested for the presence ofodor throughout 10 individual tests (i.e., T#1-T#10), as shown in Table 4. The odorwas ranked from 1 to 3 (i.e., 1 represents the minimum amount of odor, 3 represents the maximum amount of odor). The untreated substrate exhibited the highest score with an average of 2.5. The Ex. #1 and Comparative Example Comp #3 exhibited similar scores, 1.8 and 1.7, respectively. TABLE 4 No 3 3 1 2 3 1 3 3 3 3 2.5 -IIIIIIIIIII

[0106] FIG. 1 and FIG. 2 demonstrate stain reducing efficacy and urine degradation properties ofEx. #1 compared to an untreated control. In particular, FIG. 1 is a photograph ofthe untreated substrate (i.e., porcelain tile) soiled with urine. The photograph in FIG. 1 was taken 24 hours after urine dried on the substrate. FIG. 2 is a photograph ofthe substrate (i.e., porcelain tile) thatwas soiled with urine (i.e., having urine dried thereon similar to FIG. 1) and then subsequently treated with Ex. #1. The composition was applied via a mist / aerosol forming a coating on the substrate post-drying / post-cure. The substratewas allowed to cure for 24 hours. As shown in FIG. 2 andwhen compared with FIG. 1, Ex. #1 clearly reduced and / or degraded the urine and / or staining associated with urine after 24 hours compared to the untreated substrate.

[0107] The foregoing description provides embodiments ofthe invention by way of example only. It is envisioned that other embodiments may perform similar functions and / or achieve similar results. Any and all such equivalent embodiments and examples are within the scope ofthe present invention and are intended to be covered by the appended claims.

Claims

1. A liquid composition that forms a residual hydrophobic coating on a substrate after application that neutralizes urine odor and / or breaks down urine if the remaining hydrophobic coating has come into contact with urine, comprising: (a) water in a concentration of up to 80 wt.% of the liquid composition; (b) microbial spore(s) and an enzyme, dispersed, preferably homogeneous dispersed, present in the water in an effective amount to to reduce and / or eliminate urine odor and / or break down urine in a substrate after application; and (c) a film-forming compound dispersed, preferably homogeneous dispersed, present in the water in an effective amount to a to form a clear hydrophobic film on the substrate after application.

2. Liquid composition within the meaning of claim 1, where the liquid composition has a pH ranging from 5.0 to 9.8 and a viscosity ranging from 0 cPs up to 100 cPs at ambient temperature.

3. Liquid composition within the meaning of claim 1 or 2, further comprising a amalgamating agent present in an effective amount to the Tg of the to reduce film-forming compound to enable film formation after application of the liquid to enable composition on the substrate at ambient temperature.

4. Liquid composition within the meaning of claim 3, where the melting agent is an ester alcohol, a glycol ester, or a combination thereof.

5. Liquid composition within the meaning of claim 3 or 4, where the is the melting agent 2,2,4-trimethyl-1,3-pentanediol-isobutyrate.

6. Liquid composition according to one of claims 3-5, where the melting agent has a concentration ranging from 0.15 wt.% to 1.0 wt.% of an overall concentration of the liquid composition.

7. Liquid composition according to one of claims 3-6, where the film-forming compound is configured to adhere to the substrate and / or is configured to attach the microbial spore(s) and the enzyme to the substrate.

8. Liquid composition according to one of claims 3-7, where the film-forming compound is anionic, non-ionic, or a combination thereof and / or where the film-forming composition is 10 wt.% to 35 wt.% of the overall includes concentration of the liquid composition.

9. Liquid composition according to one of claims 3-8, where the film-forming compound an anionic or non-ionic acrylate, an anionic or non- comprises an ionic silicon compound, or a combination thereof.

10. Liquid composition within the meaning of claim 9, where the anionic or non- ionic acrylate is an acrylic homopolymer.

11. Liquid composition within the meaning of claim 9 or 10, where the anionic or non-ionic acrylate is present in the liquid composition in a concentration varying from 9.75 wt.% to 33.5 wt.% of the overall concentration of the liquid compound.

12. Liquid composition according to one of claims 9-11, where the film-forming composition comprises the anionic acrylate.

13. Liquid composition within the meaning of claim 12, where the anionic acrylate is an anionic acrylate homopolymer.

14. Liquid composition within the meaning of claim 12 or 13, where the anionic acrylate is present in the liquid composition in a concentration varying from 9.75 wt.% to 33.5 wt.% of the overall concentration of the liquid composition.

15. Liquid composition according to one of claims 9-14, where the film-forming composition comprises an anionic or non-ionic silicon compound.

16. Liquid composition within the meaning of claim 15, where the anionic or non- an ionic silicon compound is an organosilicon compound.

17. Liquid composition within the meaning of claim 16, where the organosilicon compound is a non-ionic organosilicon compound.

18. Liquid composition according to one of claims 15-17, where the anionic or non-ionic silicon compound is present in the liquid composition in a concentration ranging from 0.125 wt.% to 2 wt.% of the overall concentration of the liquid composition.

19. Liquid composition according to one of claims 9-18, where the film-forming compound the anionic or non-ionic acrylate and the anionic or non-ionic silicon compound comprises present in the liquid composition in ratio of 5:1 to 8:1, preferably 4:1 to 7:1 relative to each other.

20. Liquid composition according to one of claims 9-18, where the film-forming composition the anionic acrylate and the anionic silicon compound includes present in the liquid composition in a ratio of 5:1 to 8:1, at more preference 4:1 to 7:1 relative to each other.

21. Liquid composition according to one of claims 9-18, where the film-forming composition the anionic acrylate and the non-ionic silicon compound includes present in the liquid composition in a ratio of 5:1 to 8:1, at more preference 4:1 to 7:1 relative to each other.

22. Liquid composition according to one of claims 9-18, where the film-forming composition the non-ionic acrylate and the non-ionic silicon compound comprises present in the liquid composition in a ratio of 5:1 to 8:1, preferably 4:1 to 7:1 relative to each other.

23. Liquid composition according to one of claims 1-22, where the Microbial spore(s) Bacillus spores are those that break down urine.

24. Liquid composition according to one of claims 1-23, where the microbial spore(s) are present at a concentration of 2.5 x 107 to 7.5 x 107 cfu / mL, at more preference 3 x 107 to 6.75 x 107 cfu / mL in the liquid formulation.

25. Liquid composition according to one of claims 1-24, where enzyme is an anionic enzyme.

26. Liquid composition according to one of claims 1-25, where The enzyme lipase is present in an effective amount to neutralize urine and / or lipids in urine. to break off.

27. Liquid composition according to one of claims 1-26, where enzyme has a concentration ranging from 0.075 wt.% to 0.25 wt.%, preferably from 0.1 wt.% to 0.22 wt.% of the overall concentration of the liquid composition.

28. Liquid composition according to one of claims 1-27, where the microbial spore(s) and the enzyme comprise 10 wt.% to 28 wt.% of a total weight of the liquid composition.

29. A residual hydrophobic coating formed on a substrate after application of the liquid composition according to claim 1 on the substrate, whereby the remaining hydrophobic coating neutralizes urine odor and / or breaks down urine during a predetermined period specific duration 30. A residual hydrophobic coating that neutralizes urine odor and / or urine breaks off, comprising: (a) microbial spore(s) and an enzyme present in an effective amount to reduce and / or eliminate urine odor if the remaining hydrophobic coating in comes into contact with urine; and (b) a clear film with the microbial spore(s) and the enzyme composition dispersed, preferably homogeneously dispersed, therein.

31. Residual hydrophobic coating that neutralizes urine odor and / or urine degrades in accordance with claim 30, whereby the remaining hydrophobic coating is covalently bonded, electrostatically bound, or attached to the substrate by physical interaction(s).

32. Residual hydrophobic coating that neutralizes urine odor and / or urine degrades according to claim 30 or 31, where the clear film is anionic, non-ionic, or a combination thereof is.

33. Residual hydrophobic coating that neutralizes urine odor and / or urine degrades according to one of the conclusions 30-32, where the clear film 15.0 wt.% to 60 wt.%, preferably comprising 15.5 wt.% to 55 wt.% of an overall concentration of the remaining hydrophobic coating.

34. Residual hydrophobic coating that neutralizes urine odor and / or urine degrades according to one of the conclusions 30-33, where the clear film an anionic or non-ionic acrylate, an anionic or non-ionic silicon compound, or a combination includes.

35. Residual hydrophobic coating that neutralizes urine odor and / or urine breaks down according to one of the conclusions 30-34, where the anionic or non-ionic acrylate is an acrylic homopolymer.

36. Residual hydrophobic coating that neutralizes urine odor and / or urine breaks down according to one of the conclusions 30-35, where the anionic or non-ionic acrylate is present in a concentration ranging from 12.0 wt.% to 55.0 wt.% of the overall concentration of the remaining hydrophobic coating.

37. Residual hydrophobic coating that neutralizes urine odor and / or urine breaks down according to one of the conclusions 30-36, where the clear film the anionic includes acrylate.

38. Residual hydrophobic coating that neutralizes urine odor and / or urine breaks down in accordance with claim 37, where the anionic acrylate is an anionic is acrylic homopolymer.

39. Residual hydrophobic coating that neutralizes urine odor and / or urine breaks down according to claim 37 or 38, where the anionic acrylate is present in a concentration varying from 12.0 wt.% to 55.0 wt.% of the overall concentration of the remaining hydrophobic coating.

40. Residual hydrophobic coating that neutralizes urine odor and / or urine breaks down according to one of the conclusions 30-39, where the clear-film composition a includes anionic or non-ionic silicon compound.

41. Residual hydrophobic coating that neutralizes urine odor and / or urine degrades according to claim 40, where the anionic or non-ionic silicon compound a is an organosilicon compound.

42. Residual hydrophobic coating that neutralizes urine odor and / or urine degrades according to claim 40, where the organosilicon compound is non-ionic is an organosilicon compound.

43. Residual hydrophobic coating that neutralizes urine odor and / or urine breaks down according to one of the conclusions 40-42, where the anionic or non-ionic silicon compound is present in a concentration ranging from 0.4 wt.% to 5.0 wt.% of the overall concentration of the remaining hydrophobic coating.

44. Residual hydrophobic coating that neutralizes urine odor and / or urine breaks down according to one of the conclusions 30-43, where the clear film the anionic or non-ionic acrylate and the anionic or non-ionic silicon compound includes in the remaining hydrophobic coating in a ratio of 5:1 to 8:1, preferably 4:1 to 7:1 relative to each other.

45. Residual hydrophobic coating that neutralizes urine odor and / or urine breaks down according to one of the conclusions 30-43, where the clear film the anionic acrylate and the anionic silicon compound comprising present in the remaining hydrophobic coating in a ratio of 5:1 to 8:1, preferably 4:1 to 7:1 relative to each other.

46. ​​Residual hydrophobic coating that neutralizes urine odor and / or urine breaks down according to one of the conclusions 30-43, where the clear film the anionic acrylate and the non-ionic silicon compound comprising present in the remaining hydrophobic coating in a ratio of 5:1 to 8:1, preferably 4:1 to 7:1 relative to each other.

47. Residual hydrophobic coating that neutralizes urine odor and / or urine breaks down according to one of the conclusions 30-43, where the clear film the non-ionic acrylate and the non-ionic silicon compound comprising present in the remaining hydrophobic coating in a ratio of 5:1 to 8:1, preferably 4:1 to 7:1 relative to each other.

48. Residual hydrophobic coating that neutralizes urine odor and / or urine breaks down according to one of the conclusions 30-47, whereby the microbial spore(s) Baci / lus- are traces that break down urine.

49. Residual hydrophobic coating that neutralizes urine odor and / or urine breaks down according to one of the conclusions 30-48, where the microbial spore(s) are present are at a concentration of 2.5 x 107 to 7.5 x 107 cfu / mL, more preferably 3 x 107 to 6.75 x 107 cfu / mL in the remaining hydrophobic coating.

50. Residual hydrophobic coating that neutralizes urine odor and / or urine breaks down according to one of the conclusions 30-49, where the enzyme is an anionic enzyme.

51. Residual hydrophobic coating that neutralizes urine odor and / or urine breaks down according to one of the conclusions 30-50, where the enzyme a lipase is present in an effective amount to break down urine and / or lipids in urine.

52. Residual hydrophobic coating that neutralizes urine odor and / or urine breaks down according to one of the conclusions 30-51, where the enzyme has a concentration varying from 0.51 wt.% to 4.2 wt.% of the overall concentration of the remaining hydrophobic coating.

53. Residual hydrophobic coating that neutralizes urine odor and / or urine breaks down according to one of the conclusions 30-52, whereby the microbial spore(s) and the enzyme 42.7 wt.% to 83 wt.% comprising an overall weight of the remaining hydrophobic coang.

54. An article comprising: (a) a substrate; and (b) a residual hydrophobic coating according to one of claims 30-53 which neutralizes urine odor and / or breaks down urine on the substrate.

55. Article pursuant to claim 54, where the substrate is a rigid substrate, a non- porous substrate, or a combination thereof is.

56. Article pursuant to claim 54 or 55, where the substrate is glass, ceramic, metal or and a combination thereof is.

57. A method for making a liquid compound according to conclusion 1 comprising the dispersion of microbial spore(s), enzyme, and a film-forming composition in water and, if applicable, the dispersion of a melting agent therein, thereby forming the liquid composition.

58. A method comprising: (a) applying the liquid compound according to one of the claims 1028 on a substrate pursuant to one of claims 54-57 and (b) the formation of a residual hydrophobic coating according to one of the conclusions 29-53 of the substrate after application of the liquid compound, whereby the remaining hydrophobic coating neutralizes urine odor and / or breaks down urine if in brought into contact with urine. 1 / 2

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