A CYNANCHUM AURICULATUM EXOSOME, ITS EXTRACTION METHOD, AND APPLICATION

Abstract

The present invention discloses a Polygonum multiflorum exosome and its extraction method and application. The specific extraction method of the Polygonum multifiorum exosome comprises the following steps: removing the rootlets of fresh Polygonum multifiorum fruits, cutting them into pieces, juicing, collecting the filtrate, subjecting the filtrate to continuous centrifugation and differential centrifugation to obtain the supernatant, continuing to perform ultrahigh-speed centrifugation on the supernatant to collect the precipitate, and resuspending the precipitate, which is the Polygonum multifiorum exosome extract. The Polygonum multiflorum exosome obtained by the present invention is applied in the preparation of drugs for the prevention and treatment of Alzheimer’s disease. By using 3×TgAD transgenic homozygous mice, the extracted Polygonum multiflorum exosome is injected into AD mice via tail vein injection, and small animal behavioral methods, such as open field test, novel object recognition, and water maze, are employed to observe changes in the cognitive behavior of the mice, thereby verifying the improvement of cognitive impairment in AD mice by the Polygonum multiflorum exosome.