Multi-specific antigen binding molecules targeting HIV and methods of use

Abstract

A multi-specific antigen binding molecule that binds to human CD3 and an HIV antigen, wherein the antigen binding molecule comprises: (a) a first antigen binding domain that comprises a first heavy chain variable domain (VH) and a first light chain variable domain (VL), wherein the first antigen binding domain binds to CD3 and comprises: (i) a first VH-complementarity determining region (CDR) 1 comprising the amino acid sequence of TYAMN (SEQ ID NO:1); (ii) a first VHCDR2 comprising the amino acid sequence of RIRSKYNNYATYYAX1SVKX2, wherein X1 is A or D and X2 is G or S (SEQ ID NO:2); (iii) a first VH-CDR3 comprising the amino acid sequence of HGNFGX3SYVSWFAY, wherein X3 is H or N (SEQ ID NO:3); (iv) a first VL-CDR1 comprising the amino acid sequence of GSSTGAVTTGHYAN (SEQ ID NO: 4); (v) a first VL-CDR2 comprising the amino acid sequence of GTX4X5RAP, wherein X4X5 is SN or NK (SEQ ID NO:5); and (vi) a first VLCDR3 comprising the amino acid sequence of ALWYSNX6WV, wherein X6 is L or R (SEQ ID NO:6), wherein the first VH-CDR1, the first VH-CDR2, the first VH-CDR3, the first VL-CDR1, the first VLCDR2, and the first VH-CDR3 are according to Kabat; and (b) a second antigen binding domain that binds to an HIV antigen.

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