Nucleosides and nucleotides with 3' acetal blocking group

Abstract

THE PRESENT INVENTION RELATES TO A NUCLEOSIDE OR NUCLEOTIDE COMPRISING A NUCLEOBASE LINKED TO A TAGGING DETECTABLE THROUGH A CLEARABLE LINKER, WHEREIN THE NUCLEOSIDE OR NUCLEOTIDE COMPRISES A RIBOSE OR DEOXYRIBOSE 2' ENTITY AND AN OH3' LOCKING GROUP, AND WHEREIN THE CLEARABLE LINKER COMPRISES AN ENTITY OF THE STRUCTURE WHERE EACH OF X AND Y IS INDEPENDENTLY OOS; And each of R1A, R1B, R2, R3A and R3B is independently H, halogen, unsubstituted or substituted C1-C6 alkyl, or C1-C6 haloalkyl. Also provided in the present description are methods for preparing such nucleotide and nucleoside molecules, and the uses of fully functionalized nucleotides containing the 3' acetal blocking group for sequencing applications.