Modified sequence of the adenovirus e2a gene for vector production based on adenoasomiciated virus (AAV) (variants)

Abstract

THE PRESENT INVENTION RELATES TO THE FIELD OF GENETIC ENGINEERING, GENETICS, AND BIOTECHNOLOGY. MORE SPECIFICALLY, THE PRESENT INVENTION RELATES TO VARIANTS OF A MODIFIED SEQUENCE OF THE ADENOVIRUS E2A GENE, TO A HELPER PLASMID COMPRISING THE VARIANTS OF THE MODIFIED SEQUENCE OF THE ADENOVIRUS E2A GENE FOR PRODUCING ADENO-ASSOCIATED VIRUS-BASED VECTORS, AND TO THE USE OF THE VARIANTS OF THE MODIFIED SEQUENCE OF THE ADENOVIRUS E2A GENE AND OF THE HELPER PLASMIDS COMPRISING THESE SEQUENCES FOR PRODUCING ADENO-ASSOCIATED VIRUS-BASED VECTORS.