BIOTECHNOLOGICAL PHOTOSYNTHETIC SCAFFOLD OF INTERPENETRATED POLYMER NETWORK WITH ENCAPSULATED RECOMBINANT CYANOBACTERIA, CHIMERIC PROTEIN WITH INTRINSICALLY DISORDERED REGIONS AND OBTAINING PROCESS

Abstract

The present invention relates to a photosynthetic biotechnological scaffold for tissue application, comprising: a) an interpenetrating network (IPN) polymer matrix including a hydrogel polymer and a nanometric reinforcement selected from cellulose nanocrystals, cellulose nanofibers, or functional equivalents; b) a cyanobacterium encapsulated in said IPN matrix, genetically modified to express and secrete a growth factor; c) wherein said growth factor comprises a structural variant incorporating intrinsically disordered regions (IDRs), the sequential organization of which confers a propensity for liquid-liquid phase separation (LLPS) under physiological conditions; d) wherein the LLPS gives rise to a condensed protein phase within the polymer matrix; and e) wherein the cyanobacterium comprises a functional photosynthetic apparatus. The invention also relates to a method for manufacturing the photosynthetic biotechnological scaffold.