A NANO-ENHANCED LOOP-MEDIATED ISOTHERMAL AMPLIFICATIONRECOMBINASE POLYMERASE AMPLIFICATION (LAMP-RPA) BIOSENSOR KIT FOR RAPID CAGE-SIDE DETECTION OF Salmonella SPECIES IN POULTRY FARMS

Abstract

The utility model discloses a nano-enhanced loop-mediated isothermal amplification recombinase polymerase amplification (LAMP-RPA) biosensor kit for rapid cageside detection of Salmonella species in poultry farms comprising of a tube cap containing dried assay components, a tube containing Magnesium acetate (Mg(OAc)2), as an assay activator component, a tube containing Silver nano particles (AgNPs), as a rehydrating solution, a tube containing a buffer solution made of TE buffer or Tris-EDTA-HCl, for crude DNA extraction, a 200 ul sterile tubes, a 1.5 mL tubes for the crude DNA extraction, and a sterile disposable thumb press dispensing droppers. The detection was done in a single-tube reaction, enabling a rapid, equipment-free, and user-friendly nucleic acid-based diagnostic platform ideal for on site deployment. Diagnostic validation of specificity of the biosensor kit exhibited good results. Moreover, it can detect down to 64 picogram of DNA per reaction or a 10?³ dilution.