A process for the detection of quinolone-resistance strains of helicobacter pylori.

Abstract

The present invention relates to a process for detection of gyrA mutations (C261A, C261 G, G271A, G271T, and A272G) in H. Pylori genomic DNA with standard thin-walled PCR tubes, using taq polymerase comprising steps of taking 7.9 µl of distilled water, 2.5 µl of 10x reaction buffer, 2.5 ul of deoxynucleoside triphosphates (2 mM [each]), 0.5 µl of Taq polymerase, 1µl of 25 mM MgSO4, 9.6 ul of primer mixture from (a)Primer sequence 1 and 8 for the detection of C261A genotype (b) Primer sequence 2 and 8 for the detection of C261G genotype (c) Primer sequence 3 and 8 for the detection of G271A genotype (d) Primer sequence 4 and 8 for the detection of G271A genotype (e) Primer sequence 5 and 8 for the detection of G271T genotype (f) Primer sequence 6 and 8 for the detection of A2720 genotype and (g) Primer sequence 7 and 8 for the detection of A272G genotype and 1µl of H. Pylori genomic DNA.