A triplex fluorogenic probe based real-time quantitative PCR assay for the detection of SARS-COV-2 specific E protein and RdRp genes in human samples

PK144887APending Publication Date: 2026-09-10NAT INST FOR BIOTECHNOLOGY & GENETIC ENG (NIBGE)

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Patent Information

Application Number: PK202100331
Authority / Receiving Office: PK · PK
Patent Type: Applications
Current Assignee / Owner
Filing Date: 2021-04-29
Publication Date: 2026-09-10

Application Information

Patent Timeline

29 Apr 2021

Application

PK202100331

10 Sep 2026

Publication

PK144887A

Inventors: IMRAN AMIN; WAQAS RAFIQUE ALI

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Abstract

This invention relates to a triplex fluorogenic probe-based real-time quantitative PCR (RT-qPCR) assay for detection of SARS-CoV-2 in human samples. The assay simultaneously amplifies Envelope (E) gene and RNA-dependent RNA polymerase (RdRp) gene of SARS-CoV-2 along with a human RNase P internal control in a single reaction. Specific primer and probe sequences enable sensitive and specific detection suitable for in-vitro diagnostic applications.

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