Method of obtaining a collagen matrix from bones and the collagen matrix obtained by this method and use of the collagen matrix

Abstract

The subject of the application is a method for obtaining a collagen matrix from beef bones, in which the beef bones are cleaned, demineralized, sterilized and freeze-dried, which is characterized in that it comprises the following steps: a) the beef bones are cleaned of soft tissue and cut into pieces with a volume of 0.25 - 16 cm 3 , b) the raw material prepared in step a) is washed with water, filtered and dried, c) the dried raw material is subjected to the process of removing fats in the CO2 extraction process in the supercritical phase, d) the material obtained in step c) is subjected to demineralization in a hydrochloric acid solution with a concentration of 0.05 - 1 mol / dm3 3for a period of 24 hours, e) the mineral-free material obtained in step d) in the form of a raw collagen matrix is ​​washed with deionized water, f) the raw collagen matrix is ​​disinfected in a H2O2 solution for a period of 1 to 4 hours, at a volume ratio of material to water of 1:50, g) the disinfected matrix is ​​frozen to a temperature not lower than -40°C, h) the frozen material is freeze-dried. The obtained collagen matrix can be placed in a solution of an enriching substance at a concentration of 0.5 to 5% v / w. According to the method, a collagen matrix is ​​obtained in the form of porous blocks with a pore diameter of 50-500 micrometers. The collagen matrix is ​​used for the regeneration of bone defects and periodontal soft tissues.