Method of producing 11α-hydroxy-pregna-1,4-diene-3,20-dione

Abstract

The subject of the application is a method for producing 11α-hydroxy-pregn-1,4-diene-3,20-dione, characterized in that the Beauveria bassiana KCH J1.5 strain is introduced into a medium suitable for filamentous fungi, then after at least 72 hours, a substrate, which is pregn-1,4-diene-3,20-dione of formula 1, dissolved in an organic solvent miscible with water, is introduced into the culture, the transformation is carried out at a temperature of 20 to 30 degrees Celsius, with continuous shaking, for at least 12 hours, after which the product is extracted with an organic solvent immiscible with water and purified chromatographically.