Method for decellularization of adipose tissue and use of the preparation obtained by this method

Abstract

The subject of the application is a method for decellularizing adipose tissue, characterized in that a) a sample of adipose tissue is contacted with an aqueous solution containing gentamicin, b) the adipose tissue is digested with trypsin, c) the resulting mixture is extracted with isopropyl alcohol, d) optionally the obtained extract is sterilized and washed, and then the resulting decellularized adipose tissue is separated, preferably in the form of an extracellular matrix (adipoECM) preparation. The application also covers a decellularized adipose tissue preparation containing an extracellular matrix (adipoECM) obtained by the above method for use in medicine, especially plastic surgery.

Images