4'-Chloro-6-O-β-D-(4''-O-methyloglucopyranosyl)-flavanone and method of preparing 4'-chloro-6-O-β-D-(4''-O-methyloglucopyranosyl)-flavanone

Abstract

The subject of the application is 4'-chloro-6-O-α-D-(4"-O-methylglucopyranosyl)-flavanone of formula 2 and a method for producing 4'-chloro-6-O-α-D-(4"-O-methylglucopyranosyl)-flavanone, characterized in that the Beauveria bassiana KCH J1.5 strain is introduced into a medium suitable for filamentous fungi, then after at least 72 hours a substrate is introduced into the culture, which is 4'-chloroflavanone of formula 1, dissolved in an organic solvent miscible with water, the transformation is carried out at a temperature of 20 to 30 degrees Celsius, with continuous shaking, for at least 96 hours, then the product is extracted with an organic solvent immiscible with water and purified chromatographically, wherein 4'-chloro-6-O-α-D-(4"-O-methylglucopyranosyl)-flavanone of formula 2 is found in the fraction of intermediate polarity, in the third band from the starting line.