COMPOSITIONS BASED ON FEED ADDITIVES AND METHODS OF THEIR APPLICATION

Claims

1. A feed additive composition comprising or essentially consisting of a probiotic (DFM) comprising Bifidobacterium animal is subsp. lactis and / or Lactobacillus acidophilus.

2. A composition based on a feed additive according to paragraph 1, additionally containing or essentially consisting of an osmoregulator.

3. A composition based on a feed additive according to claim 1 or 2, additionally containing or essentially consisting of at least one essential oil.

4. A composition based on a feed additive according to any one of claims 1 to 3, further comprising or essentially consisting of one or more enzymes selected from the group consisting of protease, xylanase, beta-glucanase, phytase and amylase.

5. A composition based on a feed additive according to claim 4, wherein the enzyme is encapsulated, or is present in the form of a granule, or is lyophilized.

6. A composition based on a feed additive according to any one of claims 1 to 5, containing or essentially consisting of the B1-04 strain of Bifidobacterium animal is subsp. lactis.

7. A composition based on a feed additive according to any one of claims 1 to 6, containing or essentially consisting of the NCFM strain of Lactobacillus acidophilus.

8. A composition based on a feed additive according to any one of paragraphs 1-7, wherein the osmoregulator comprises betaine.

9. A composition based on a feed additive according to any one of claims 1-8, wherein at least one essential oil comprises cinnamaldehyde, carvacol and / or thymol.

10. A composition based on a feed additive according to any one of claims 1 to 9, further comprising or essentially consisting of at least one additional DFM.

11. A composition based on a feed additive according to any one of claims 1-10, further comprising or essentially consisting of one or more of aspartic acid (aspartate), ornithine, arginine, phosphate, acetate and / or vitamin B.

12. A composition based on a feed additive according to any one of paragraphs 1-11, wherein vitamin B is one or more of vitamin B1, B6 and / or B 12 .

13. A feed additive composition according to any one of claims 1 to 12, wherein at least one component of the composition is formulated for delivery through a water supply.

14. Feed or premix for animals containing a composition based on a feed additive according to any of paragraphs 1-12.

15. A method for treating or preventing necrotic enteritis in a subject in need thereof, comprising administering to the subject an effective amount of a composition based on a feed additive according to any one of claims 1-13 or an animal feed or premix according to claim 14.

16. The method according to claim 15, wherein the subject is a farm bird or a domestic pig.

17. The method according to claim 15 or 16, wherein the poultry is a broiler, a layer, or a turkey.

18. The method according to claim 15 or 16, wherein the domestic pig is a piglet, a growing pig, or a sow.

19. The method according to any one of paragraphs 15-18, wherein said method provides for the reduction or prevention of intestinal lesions caused by necrotic enteritis.

20. The method of any one of claims 15-19, wherein said method further provides a reduction in feed conversion ratio, an increase in feed efficiency, a reduction in mortality, an increase in final slaughter weight, or an increase in weight gain in a subject compared to a subject that has not been administered an effective amount of the composition based on the feed additive of any one of claims 1-13 or the animal feed or premix of claim 14.

21. The method of claim 20, wherein the subject has clinical or subclinical necrotic enteritis.

22. The method according to any one of claims 15-21, wherein said method further comprises reducing the expression of necrotic enteritis toxin B-like (NetB) in Clostridium perfringens.

23. The method according to any one of paragraphs 15-22, wherein the composition based on the feed additive is introduced via a water supply.

24. The method according to any one of paragraphs 15-23, wherein said administration is carried out without co-administration of an antibiotic to the subject.

25. A method for treating or preventing coccidiosis in a subject in need thereof, comprising administering to the subject an effective amount of a composition based on a feed additive according to any one of claims 1-13 or an animal feed or premix according to claim 14.

26. The method according to claim 25, where the subject is poultry.

27. The method according to claim 25 or 26, wherein the poultry is a broiler or a layer.

28. The method according to any one of paragraphs 25-27, wherein said method provides a reduction in feed conversion ratio, an increase in feed efficiency, a reduction in mortality, an increase in final slaughter weight, or an increase in weight gain in a subject.

29. The method according to any one of claims 25-28, wherein said method provides for a reduction in the number of one or more intestinal species of Eimeria.

30. The method according to any one of paragraphs 25-29, wherein at least one component of the composition based on a feed additive is introduced via a water supply.

31. A method for reducing the expression of necrotic enteritis B-like toxin (NetB) in Clostridium perfringens, comprising contacting a C. perfringens cell with one or more of aspartic acid (aspartate), ornithine, arginine, phosphate, acetate, vitamin B, and / or the secretomes of one or both of B. animalis or L. acidophilus.

32. The method according to claim 31, wherein vitamin B is one or more of vitamin B1, B6 and / or B 12 .

33. The method according to claim 31 or 32, wherein B. animalis is the B1-04 strain of Bifidobacterium animalis subsp. lactis, and / or L. acidophilus is the NCFM strain of Lactobacillus acidophilus.

34. The method according to any one of claims 31-33, wherein the C. perfringens cell is located in the intestine of a poultry.

35. The method according to claim 34, wherein the poultry is a chicken, quail, duck, goose, emu, ostrich, pheasant or turkey.

36. The method according to claim 35, wherein the chicken is a broiler or a layer.

37. The method according to any one of paragraphs 31-36, further comprising contacting the C. perfringens cell with one or more osmoregulators and / or at least one essential oil.

38. The method according to claim 37, wherein the osmoregulator comprises betaine.

39. The method according to claim 37 or 38, wherein at least one essential oil comprises cinnamaldehyde, carvacol and / or thymol.

40. A method for reducing the expression of necrotic enteritis toxin B-like (NetB) in Clostridium perfringens in a subject in need thereof, comprising or essentially consisting of administering to the subject an effective amount of a composition based on a feed additive according to any one of claims 1-13 or an animal feed or premix according to claim 14, wherein the C. perfringens cell is located in the intestine of the subject.

41. The method according to claim 40, wherein the subject is a farm bird or a domestic pig.

42. The method according to claim 40 or 41, wherein the poultry is a broiler, a layer, or a turkey.

43. The method according to claim 40 or 41, wherein the domestic pig is a piglet, a growing pig, or a sow.

44. The method according to any one of paragraphs 40-43, wherein said method provides for the reduction or prevention of intestinal lesions caused by necrotic enteritis.

45. The method of any one of claims 40-44, wherein said method further provides a reduction in feed conversion ratio, an increase in feed efficiency, a reduction in mortality, an increase in final slaughter weight, or an increase in weight gain in a subject compared to a subject that has not been administered an effective amount of the composition based on the feed additive of any one of claims 1-13 or the animal feed or premix of claim 14.

46. ​​The method of claim 45, wherein the subject has clinical or subclinical necrotic enteritis.

47. The method according to any one of paragraphs 40-46, wherein the composition based on the feed additive is introduced via a water supply.

48. The method according to any one of paragraphs 40-47, wherein said administration is carried out without co-administration of an antibiotic to the subject.