METHOD FOR DETECTING THE PATHOGENIC BACTERIA VIBRIO CHOLERAE
Patent Information
- Authority / Receiving Office
- RU · RU
- Patent Type
- Applications
- Current Assignee / Owner
- FEDERAL STATE AUTONOMOUS EDUCATIONAL INSTITUTION OF HIGHER EDUCATION URAL FEDERAL UNIV NAMED AFTER THE FIRST PRESIDENT OF RUSSIA B N YELTSIN
- Filing Date
- 2024-12-27
- Publication Date
- 2026-06-29
Claims
A method for detecting the pathogenic bacterium Vibrio cholerae, which includes taking a sample from the food being studied, adding to it a functional component that affects the change in the EPR characteristics of the sample, measuring the EPR spectrum and determining the presence of the pathogenic bacterium in the sample, characterized in that the nucleic acid sequence UUCCAAUGGUUAUCCCCCUC with the spin label TEMPO is used as the functional component, and to determine the presence of Vibrio cholerae in the sample, the following changes in magnetic resonance parameters are detected using the EPR spectra: a change in the rotational correlation (τ с ) TEMPO radical with (3.0±0.30)×10 -11 up to (1.1±0.11)×10 -10 ; changes in the components of the rotational diffusion coefficient of the TEMPO radical, with -1 :D x with (1.8±0.18)×10 10 up to (2.9±0.29)×10 9 ; D y and D z with (7.0±0.70)×10 8 up to (6.4±0.64)×10 8 .