Composition and method for producing a hydrogel composition for bioprinting

Claims

1. A biocompatible hydrogel composition for 3D bioprinting, intended for the creation of tissue-engineered constructs with living human or mammalian cells, containing: 1) sodium alginate at a concentration of 1.5% (weight / volume); 2) gelatin at a concentration of 1% (weight / volume); 3) distilled water as a solvent, 2. A method for producing a biocompatible hydrogel composition, comprising: 1) dissolving sodium alginate and gelatin in distilled water at a temperature of 50°C with stirring until completely dissolved; 2) sterilization of the resulting solution by autoclaving at a temperature of 121°C.

3. Use of a biocompatible hydrogel composition according to paragraph 1 for: 1) extrusion 3D bioprinting of tissue-engineered structures; 2) cultivation of living human or mammalian cells; 3) regenerative medicine, oncology research, pharmaceutical production.

4. A method for bioprinting tissue-engineered structures, including: 1) mixing the hydrogel composition according to paragraph 1 with a cell suspension in a ratio of 1:1 by volume; 2) placing the resulting mixture into a cartridge for extrusion bioprinting; 3) printing of bioconstruct; 4) cross-linking the printed structure with a sterile CaCl2 solution at a concentration of 10 mM.