Method for indirect determination of concentration of glycoprotein of arriah strain of fixed rabies virus in raw materials for vaccines

A PCR-based method using specific primers and probes effectively determines glycoprotein concentration in rabies virus raw materials, addressing sensitivity and specificity issues, achieving rapid and accurate analysis.

RU2864787C1Active Publication Date: 2026-06-29FEDERALNOE GOSUDARSTVENNOE BIUDZHETNOE UCHREZHDENIE FEDERALNYI TSENTR OKHRANY ZDOROVIA ZHIVOTNYKH FGBU VNIIZZH
View PDF 2 Cites 0 Cited by

Patent Information

Authority / Receiving Office
RU · RU
Patent Type
Patents
Current Assignee / Owner
FEDERALNOE GOSUDARSTVENNOE BIUDZHETNOE UCHREZHDENIE FEDERALNYI TSENTR OKHRANY ZDOROVIA ZHIVOTNYKH FGBU VNIIZZH
Filing Date
2025-09-15
Publication Date
2026-06-29

AI Technical Summary

Technical Problem

Existing methods for determining the concentration of glycoprotein in rabies virus raw materials for vaccines, such as ELISA, are limited by sensitivity, specificity, and contamination risks, and do not effectively separate native proteins from biologically inactive fragments.

Method used

A PCR-based method using oligonucleotide primers and a molecular probe to indirectly determine the concentration of glycoprotein in rabies virus raw materials, involving RNA extraction, reverse transcription, and real-time PCR with a developed linear function to calculate glycoprotein concentration based on the threshold amplification cycle.

Benefits of technology

The method reduces analysis time to 3 hours, increases sensitivity to 0.01 μg/ml, ensures high specificity, and provides a reliable correlation between glycoprotein concentration and threshold amplification cycle, with an amplification efficiency of 99.48% and approximation reliability of 0.997.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure 00000001
    Figure 00000001
Patent Text Reader

Abstract

FIELD: biotechnology.SUBSTANCE: quality control of raw materials in the manufacture of a culture-based inactivated anti-rabies vaccine, namely a method for indirectly determining the concentration of the glycoprotein of the ARRIAH strain of the fixed rabies virus in raw materials for vaccines. The main advantages of the proposed invention are the ability to reduce the analysis time of virus-containing suspensions when determining the concentration of the glycoprotein of the ARRIAH strain of the rabies virus in the raw material for the vaccine to 3 hours; by using original highly specific primers and a molecular probe, to achieve analytical sensitivity of up to 0.01 μg / ml; to increase the reliability of the analysis by establishing a relationship between the concentration of the glycoprotein of the ARRIAH strain of the fixed rabies virus (CRVG) and the threshold amplification cycle (Ct), presented as a linear function CRVG= –0.228Ct+ 7.615, where CRVG is the concentration of the glycoprotein of the ARRIAH strain of the fixed rabies virus, Ct is the threshold amplification cycle with high approximation reliability (R2 = 0.997) and amplification efficiency (E) of 99.48%.EFFECT: proposed model allows for indirect determination of the concentration of the glycoprotein of the ARRIAH strain of the rabies virus in vaccine raw materials using the developed method.2 cl, 1 dwg, 6 tbl, 6 ex
Need to check novelty before this filing date? Find Prior Art