Method for detecting methylation states and mutations in DNA sample

Abstract

FIELD: molecular biology.SUBSTANCE: method for detecting the methylation and mutation states of a DNA sample is described and, in particular, a method for simultaneously detecting the methylation state and mutation state of a DNA molecule in a sample is provided. The method includes treating a sample with a methylation-sensitive restriction endonuclease; using a group of probes, including a mutation capture probe and a methylation capture probe, to perform hybrid capture of amplification products of a sample treated with a restriction endonuclease and a sample without treatment by enzymatic cleavage; and sequencing the capture product.EFFECT: development of a means that ensures the joint detection of mutation and methylation in the same hybrid capture system.8 cl, 6 dwg, 11 tbl, 1 ex

Description

[0001] This application claims priority to Chinese Patent Application No. 2023107826914, filed with the National Intellectual Property Administration of China on June 29, 2023, the entire contents of which are incorporated herein by reference.Technical Field

[0002] The present application relates to a method for simultaneously detecting methylation and mutation states, wherein the detection of methylation and / or mutation in a target region is carried out by capture.Background of the invention

[0003] According to statistics, in 2022, approximately 4.82 million new cancer cases and 3.21 million cancer deaths were reported in China. Cancer has become a globally recognized public health problem. Early screening and diagnosis of cancer are the most important way to reduce cancer mortality. According to a report on early detection of multiple cancers published by Grand View Research, Inc., the global market size of early detection of multiple cancers is expected to reach USD 2.77 billion by 2030, expanding at a CAGR of 15.80% from 2022 to 2030. The emergence of early detection technologies for multiple cancers has epochal significance for early screening and early diagnosis of cancer. Compared with single-cancer detection, multiple-cancer detection can enable the detection of multiple cancers in a single detection procedure and has become a direction for the future development of the industry.With the development of the early cancer screening industry and the expansion of the market size, more and more organizations are now focusing on the field of early cancer screening, moving from single cancer detection to multiple cancer detection.

[0004] Early clinical tumor screening methods include imaging, endoscopy, and tumor markers. These detection technologies have disadvantages such as invasiveness, discomfort during screening, and low detection sensitivity. Standard methods have various limitations. There is an urgent clinical need for early screening technologies with high sensitivity / specificity and ease of use. Liquid biopsy, as a new noninvasive detection technology, has great potential for early tumor screening. Tumor markers for liquid biopsy mainly include genomics, epigenetics, transcriptomics, microbiomics, proteomics, and metabolomics. Tumor marker characteristics at the ctDNA level include measurements such as point mutations, fragmentation, copy number variation, and methylation.Mutation detection is the most advanced technology for gene detection companies. However, early mutational signals of cancer are weak and very difficult to detect, and they are susceptible to interference from clonal hematopoiesis signals, leading to problems such as a lack of tumor specificity and difficulty tracing tumor origins. Methylation markers, characterized by stable presence in blood plasma, multiple sites of variation, the ability to trace tissue of origin, etc., have become the markers of choice in early cancer screening. Cancer occurrence and progression are associated with changes at multiple levels, and therefore, assessments at a single level are limited. Thus, "multi-omics" has become one of the most popular technical terms in the field of tumor detection for early cancer screening.The combination of mutation and methylation detection technologies provides complementary benefits, facilitating the implementation of early screening technologies for multiple cancer types.

[0005] There are various methods for methylation sequencing. First-generation and second-generation sequencing technologies can only distinguish between four bases (A, T, C, and G) and cannot distinguish between methylated and unmethylated C bases. The standard processing scheme is to use bisulfite to convert the unmethylated C base to a U base, convert the U base to a T base through PCR amplification, and compare it with the unconverted sequence to determine whether methylation has occurred at the site. However, since the converted sequence differs from the original sequence, the liquid-phase hybrid capture scheme based on existing systems cannot achieve the experimental goal of jointly detecting mutation and methylation in the same hybrid capture system, resulting in simultaneous methylation and mutation detection.

[0006] BRIEF DESCRIPTION OF THE INVENTION

[0007] This document provides a method for simultaneously detecting the methylation state and mutation state of a DNA molecule in a sample, comprising:

[0008] 1) adding adapter molecules to both ends of a DNA molecule in a sample to obtain an adapter ligation product sample; and dividing the adapter ligation product sample into two parts: a first adapter ligation product sample and a second adapter ligation product sample;

[0009] 2) treating a first sample of the adapter ligation product with one or more methylation-sensitive restriction endonucleases so that the DNA molecule is capable of undergoing an enzymatic cleavage reaction if methylation is not present at the recognition site of the methylation-sensitive restriction endonuclease, thereby obtaining a sample of the enzymatic cleavage product; and not treating a second sample of the adapter ligation product with a restriction endonuclease;

[0010] 3) amplifying the sample of the enzymatic cleavage product and the second sample of the adapter ligation product, respectively, using a primer specific to the adapter molecule and having an index sequence, to obtain an amplification product of the sample of the enzymatic cleavage product and an amplification product of the second sample of the adapter ligation product, respectively; and mixing the amplification product of the sample of the enzymatic cleavage product with the amplification product of the second sample of the adapter ligation product to obtain a mixture of amplification products;

[0011] 4) contacting a probe group comprising one or more mutation capture probes and one or more methylation capture probes with the mixture of amplification products to obtain a capture product, wherein the methylation capture probe targets a target sequence containing a recognition site in a DNA molecule, and the mutation capture probe targets a target sequence containing a mutation site in a DNA molecule; and

[0012] 5) sequencing the capture product; determining the methylation state of the DNA molecule containing the recognition site using the ratio of the sequencing depth of the recognition sites in the amplification product of the enzymatic cleavage product sample and the amplification product of the second adapter ligation product sample; and determining the mutation state based on the sequencing results of the mutation site.

[0013] In some embodiments, the capture probe for mutation and / or the capture probe for methylation comprise a target-specific sequence, a first probe-binding sequence located at the 5'-end of the target-specific sequence, and a second probe-binding sequence located at the 3'-end of the target-specific sequence; and the first probe-binding sequence is at least partially complementary to the second probe-binding sequence, so that if two or more capture probes for mutation and / or two or more capture probes for methylation bind to their target sequence adjacently, the adjacent capture probes for mutation and / or adjacent capture probes for methylation are capable of complementarily binding via the first probe-binding sequence and the second probe-binding sequence.

[0014] In some embodiments, the probe group further includes one or more normalizing probes.

[0015] In some embodiments, the first probe binding sequence and the second probe binding sequence are 8-30 nt in length.

[0016] In some embodiments, the target-specific sequence is 20-80 nt in length.

[0017] In some embodiments, the method, before step 1), further comprises fragmenting the DNA molecule.

[0018] In some embodiments, the methylation-sensitive restriction endonuclease is selected from Hpa I, Hpa II, Hha I, Aci I, and any combination thereof.

[0019] In some embodiments, the sample comprises ctDNA.

[0020] In some embodiments, the methylation state includes the presence of methylation and / or the level of methylation.

[0021] In some embodiments, the mutation state includes the presence of a mutation and / or a type of mutation.

[0022] In some embodiments, the mutation type is selected from insertion, deletion and substitution of a base, a change in the number of chromosomal copies, microsatellite instability and gene fusion. Brief description of the drawings

[0023] Fig. 1 shows the method for obtaining the capture library.

[0024] Fig. 2 shows the experimental method for the combined detection of capture and mutation and the corresponding design scheme of the probe. (A): The experiment is started according to the experimental method described in Fig. 1; after obtaining the adapter ligation product, a methylation-sensitive restriction endonuclease is used for enzymatic digestion, the libraries obtained from the enzymatic digestion product are used to detect the methylation state, and the libraries obtained without enzymatic digestion are used to detect mutations. (B): The experiment is started according to the experimental method described in Fig. 1; after obtaining the adapter ligation product, a methylation-sensitive restriction endonuclease is used for enzymatic digestion, the libraries obtained from the enzymatic digestion product are used to analyze the methylation state and detect the mutation state.(C): Probe design concept: methylation detection is performed, and the probe must span the cleavage site of a methylation-sensitive restriction endonuclease; mutation site detection is performed, and the probe must span the genetic site to be detected. If the experimental method from B is used, the design of the mutation probe must take into account methylation detection, and the designed probe cannot span the cleavage site of a methylation-sensitive restriction endonuclease.

[0025] Fig. 3 shows a comparison of the experimental methods for mutation and methylation detection between the standard detection scheme and the scheme used in this application. (A): A method for mutation and methylation detection using the standard detection scheme, where two sets of libraries are prepared and two sets of hybrid capture experiments are carried out. The methylation library uses a methylation-modified adapter with a relatively high adapter cost. The ligation product of the adapter is processed by a BS or EM conversion module to convert the unmethylated C base to a U base.(B): A scheme for the combined detection of mutation and methylation according to the present application, where a common DNA adapter is used; MSRE is used for enzymatic cleavage; methylation-modified and non-methylation-modified C bases are distinguished; in this system, a single DNA library is prepared, or the adapter ligation product is divided into two parts: one is enzymatically cleaved and then amplified, and the other is directly amplified; both amplification products can be hybridized in the same hybrid capture system.

[0026] Fig. 4 shows the experimental method and the quality control results after analysis. (A) Experimental method. (B) Efficiency test results: hit is the proportion of target reads in mappable reads; mappability is the mapping level of data in the genome; (C) 0.5× average coverage is the percentage of 0.5× average coverage; (C) 0.2× average coverage is the percentage of 0.2× average coverage.

[0027] Fig. 5 shows the sequencing depths and the corresponding methylation levels of CpG sites associated with methylation-sensitive restriction endonuclease in the target regions of different libraries, where the methylation level calculation method is: digestion depth / control depth; (A) The average CpG methylation level of two experimental digestion groups; (B) The methylation level corresponding to each CpG site.

[0028] Fig. 6 shows the results of the agreement analysis between the mutation detection rates and the theoretical mutation rates in the capture data. Detailed description of the embodiments

[0029] Unless otherwise defined, all technical and scientific terms used herein have the meanings commonly understood by those skilled in the art.

[0030] The term "or" refers to one of the listed selectable elements, unless the context clearly indicates otherwise. The term "and / or" refers to any one, any two, any three, any more, or all of the listed selectable elements.

[0031] The terms "comprising," "including," "having," and similar expressions used in this document are terms that do not exclude elements that are not listed. These terms also include examples consisting solely of the listed elements.

[0032] The "DNA molecule" in this document refers to deoxyribonucleic acid, which is a polymer of deoxyribonucleotides. It may be in double-stranded form or may be in single-stranded form, or in the case of a population of DNA molecules, it may include a double-stranded DNA molecule or may include a single-stranded DNA molecule. The DNA molecule may be of any length, for example, it may be genomic DNA, a DNA fragment, or extracellular free DNA. Free DNA refers to DNA that is free outside a cell or cell nucleus and can be extracted from biological materials such as various biological fluids, in vitro cell culture fluids, and natural environments, including but not limited to peripheral blood, blood plasma, blood serum, urine, feces, saliva, cerebrospinal fluid, and lymphatic fluid. Free DNA can be obtained through extraction and purification.A "DNA fragment" as used herein refers to a shorter DNA molecule, for example, between 50 bp and 700 bp in length, such as between 100 bp and 500 bp, and in particular between 100 bp and 350 bp. DNA fragments contained in a biological sample are typically heterogeneous, i.e., they vary in length. Accordingly, the above-mentioned lengths may refer to the average length of these DNA fragments. These DNA fragments may have different sequences, for example, originating from different regions of the genome of the same organism or even originating from different organisms. DNA fragments may have single-stranded cuts or may have blunt ends or non-blunt ends (with 3' overhanging ends or 5' overhanging ends).

[0033] “DNA methylation” as used herein refers to the methylation of bases in DNA molecules or DNA fragments, specifically the modification of cytosine to 5-methylcytosine (5 mC). DNA methylation in vertebrates typically occurs at CpG sites (i.e., sites following cytosine and closely associated with guanine in DNA sequences), where cytosine is converted to 5-methylcytosine by DNA methyltransferase. Most CpG sites in the human genome are already methylated. However, some specific regions, such as CpG islands enriched in cytosine (C) and guanine (G), are typically unmethylated. CpG methylation can influence the transcriptional activity of associated genes. For example, methylation can suppress cancer suppressor genes, while demethylation can stimulate the expression of certain oncogenes, both of which can cause cancer.Additionally, although less common than 5mC, a small number of cytosine bases are modified to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxycytosine (5caC). References in this document to methylation may also refer to modifications at 5hmC, 5fC, and 5caC unless the context otherwise indicates.

[0034] “Methylation state of a cytosine base” or “DNA methylation information” as used herein refers to information about the methylation state of a DNA molecule or DNA fragment, including, but not limited to, methylation sites, methylation levels, and methylation modes (e.g., 5 mC or 5 hmC). “Methylation level,” also referred to as “degree of methylation,” refers to the proportion (or frequency) of the methylated modification of a particular methylation site in a sample. Various methods can be used to determine whether a site is methylated. Common methods include chemical or enzymatic conversion methods, where either methylated cytosine or unmethylated cytosine is converted to uracil (U) or a base (e.g., dihydrouracil, DHU) substantially equivalent to uracil in base pairing, during the conversion.During the subsequent amplification process, the corresponding uracil, as thymine (T), pairs with adenine (A), ultimately resulting in cytosine or methylated cytosine at the methylation site being represented as thymine in detection results (e.g., sequencing results). By comparing with a reference sequence, it is possible to determine whether the cytosine in a DNA molecule or DNA fragment is methylated. The reference sequence may be a sequence obtained from the same sample without the above-mentioned transformation or may represent the corresponding sequence in healthy populations. Certain methods can also distinguish between 5mC and 5hmC. DNA methylation information is currently widely used in cancer screening and diagnosis (e.g., lung, breast, liver, and colorectal cancer), particularly in early screening and diagnosis.Furthermore, methylation status identification can also be used for non-diagnostic (or non-therapeutic) purposes, such as being used for research (e.g., to analyze factors influencing methylation and the impact of methylation on gene function), or being used to identify methylation standards.

[0035] "Methylation-sensitive restriction endonuclease" refers to a restriction endonuclease that is sensitive to whether its recognition site contains a methylated base. Such enzymes generally cannot cleave DNA molecules at their recognition sites if their recognition sites contain a methylated base. A methylation-sensitive restriction endonuclease in this document specifically refers to a restriction endonuclease that cannot cleave a DNA molecule at its recognition site if the recognition site contains at least one 5mC. Methylation-sensitive restriction endonucleases known in the art include, but are not limited to, Hpa I, Hpa II, Hha I, and Aci I.

[0036] "Capture probe" in this document refers to a single-stranded DNA fragment used for hybridization with a target DNA molecule. The capture probes in this document include a methylation capture probe and a mutation capture probe. The methylation capture probe used is preferably targeted to a target sequence containing a restriction endonuclease recognition site in the DNA molecule. When a DNA molecule is treated with a specific restriction endonuclease, if the methylation state of its recognition site allows the restriction endonuclease to cleave the DNA molecule, the capture probe may only partially or insufficiently bind to the target DNA molecule, thereby preventing efficient recovery of the restriction endonuclease-cleaved target DNA molecule from the sample.Under these conditions, the target DNA molecules that can be isolated from restriction endonuclease-treated samples using a methylation capture probe are primarily DNA molecules that are not cleaved by the restriction endonuclease. Therefore, the overall methylation status of DNA molecules in a sample can be determined based on the number of undigested DNA molecules that can be isolated, compared to the number of DNA molecules that can be isolated from samples not treated with the restriction endonuclease. For example, when treated with a methylation-sensitive restriction endonuclease, the number of cleaved DNA molecules is relatively high (the number of undigested DNA molecules is relatively low), indicating that the DNA molecules in the sample have a lower methylation level at the restriction endonuclease recognition site.In a preferred embodiment, the methylation capture probe is a μCaler probe (for the design of the μCaler probe, see Chinese Patent Publication CN 116083423 A, the entire contents of which are incorporated herein by reference). The probe comprises a target-specific sequence, a first probe-binding sequence located at the 5'-end of the target-specific sequence, and a second probe-binding sequence located at the 3'-end of the target-specific sequence. The first probe-binding sequence is at least partially complementary to the second probe-binding sequence, so that when two or more capture probes are adjacently bound to a target sequence, the adjacent capture probes can be complementarily linked via the first probe-binding sequence and the second probe-binding sequence.Compared with conventional capture probes, the advantages of using the μCaler probe include significantly improved target binding ability, increased hybrid capture efficiency of the target region, and improved overall coverage uniformity and stability. The capture probe typically has a label that enables isolation, such as biotin, to facilitate the isolation of target DNA molecules that bind to the probe. Sequence information on the various isolated target DNA molecules can be obtained by sequencing, and the methylation status of the restriction endonuclease recognition site can also be determined. A mutation capture probe is a capture probe that targets a mutation site of interest contained in a DNA molecule, and information on the mutation status of a specific mutation site can be obtained by sequencing the DNA molecules captured by the mutation capture probe.Preferably, the mutation capture probe is also a μCaler probe. The methylation capture probe and the mutation capture probe can be located in the same probe group, ensuring simultaneous capture of the corresponding target sequences. Furthermore, the probe group used herein can also include a normalization probe to normalize detection results between different samples. Preferably, the normalization probe is also a μCaler probe.

[0037] In some embodiments, the present document provides a method for producing a methylation-targeted library based on a methylation-sensitive restriction endonuclease, and the methylation-sensitive restriction endonuclease comprises Hpa I, Hpa II, Hha I, and Aci I.

[0038] In some embodiments, the targeted capture system provided herein is for capturing an untransformed library; the original DNA sequences are captured; methylation modification occurs at all CpG sites of the restriction endonuclease recognition sites in the target regions of the captured fragments; information about the methylation modification occurring in the target regions is detected.

[0039] In some embodiments, the method provided herein is used to detect mutations in a DNA sample, and the types of mutations include: base substitution, chromosomal copy number change analysis, insertion / deletion, microsatellite instability, or gene fusion.

[0040] In some embodiments, the capture probes used are designed for the original DNA strands and detect sites associated with methylation, and the targeted capture probe should encompass the corresponding methylation-sensitive restriction endonuclease cleavage site.

[0041] In some embodiments, two different libraries are used for the combined detection of mutation and methylation, respectively, and the design of the capture probe for mutation does not need to take into account the design of the capture probe for methylation. In some embodiments, the same library is used for the combined detection of mutation and methylation, and the capture probe for mutation cannot cover the methylation-sensitive restriction endonuclease recognition sequence associated with methylation detection.

[0042] In some embodiments, the present document provides a method for producing a targeted methylation library based on a methylation-sensitive restriction endonuclease; the sequencing depth of CpG sites can be detected using the method; the methylation degree of CpG sites in a sample is analyzed using a control.

[0043] In some embodiments, the method provided herein uses an adapter module containing a molecular label, and the molecular label provides background noise filtering and is suitable for detecting a low-frequency mutation signal.

[0044] For the method for producing the targeted capture library in this document, see Fig. 1. The method is suitable for detecting methylation states and mutation signals in target regions in a physically disrupted gDNA sample and a free plasma DNA sample or circulating tumor ctDNA. Fragmented samples are used for library preparation, and the library preparation kit is a Nadprep Universal DNA Library Preparation Kit. The library preparation method is divided into two schemes. Workflow A. End repair and A-tailing, and adapter ligation. The adapter ligation product is subjected to enzymatic digestion with a methylation-sensitive restriction endonuclease. The restriction endonuclease types include Hpa I, Hpa II, Hha I, and Aci I. The other group is not subjected to enzymatic digestion to serve as a control group.Two product groups are respectively subjected to PCR amplified with the addition of an index sequence. The amplified products are subjected to hybrid capture using the μCaler Hybrid Capture System. The μCaler probe design scheme is used for all probes. Probes are designed for the original DNA sequences. The probes span the methylation-sensitive restriction endonuclease cleavage site in case of methylation detection and span the mutation detection site in case of mutation signal detection. Workflow B. A methylation-sensitive restriction endonuclease is used for enzymatic digestion. Restriction endonuclease types include Hpa I, Hpa II, Hha I, and Aci I. The enzymatic digestion products are purified and recovered. Library preparation is then performed. The products for library preparation are subjected to hybrid capture using the μCaler Hybrid Capture System. The μCaler probe design scheme is used for all probes.Probes are designed for the original DNA sequences. The probes span the methylation-sensitive restriction endonuclease cleavage site if a methylation state is detected, and span the mutation detection site if a mutation signal is detected. Captured libraries are sent to an Illumina or MGI sequencer for sequencing.

[0045] For the experimental method for the combined detection of capture and mutation and the corresponding experimental design scheme, see Fig. 2. A: Start the experiment according to the experimental method described in Fig. 1; after obtaining the adapter ligation product, use a methylation-sensitive restriction endonuclease for enzymatic digestion, use the libraries obtained from the enzymatic digestion product to detect the methylation state, and use the libraries obtained without enzymatic digestion to detect mutations. B: Start the experiment according to the experimental method described in Fig. 1; after obtaining the adapter ligation product, use a methylation-sensitive restriction endonuclease for enzymatic digestion, use the libraries obtained from the enzymatic digestion product to analyze the methylation state and detect the mutation state.C: The concept of probe design in this system is as follows: methylation detection is performed, with the probe spanning the cleavage site of a methylation-sensitive restriction endonuclease; mutation site detection is performed, with the probe spanning the genetic site to be detected. If the experimental method in B is used, the design of the mutation detection probe must take methylation detection into account, and the designed probe cannot span the cleavage site of a methylation-sensitive restriction endonuclease.

[0046] The use of this system for the method of combined mutation and methylation detection has significant advantages over the standard scheme, as detailed in Fig. 3, which shows a comparison of the experimental methods for mutation and methylation detection between the standard detection scheme and the scheme used in this system. In the method of mutation and methylation detection using the standard detection scheme (Fig. 3A), it is necessary to prepare two sets of libraries and conduct two sets of hybrid capture experiments. The methylation library uses a methylation-modified adapter, which has a relatively high adapter cost.The adapter ligation product is processed using the BS (running time 2 hours) or EM transformation module (running time 8 hours). The BS transformation process is relatively cumbersome and is characterized by relatively high transformation damage, so using this scheme leads to a significant loss of information in the original sample. The EM transformation module is characterized by relatively low transformation damage, and it is recommended to use the NEBNext enzymatic transformation module to convert the unmethylated C base to the U base and to use an amplification enzyme (KAPA HiFi HotStart Uracil Mix) capable of recognizing the U base for PCR amplification. The mutation detection library uses a common adapter module.Given that the transformed DNA sequences of the library are modified, the probe used during hybrid capture is designed for the transformed DNA sequences. The methylation library and the DNA library must use independent hybrid capture systems to perform the experiments. In the scheme (Fig. 3B) of combined mutation and methylation detection of this system, a common DNA adapter is used; MSRE is used for enzymatic digestion (experimental time is 1 hour); methylation-modified and non-methylation-modified C bases are distinguished; in this system, a single DNA library is obtained, or the adapter ligation product is divided into two parts: one is enzymatically digested and then amplified, and the other is directly amplified; both amplification products can be hybridized in the same hybrid capture system.Using the standard mutation and methylation detection scheme may take 2-3 days, while using the scheme of this system can reduce the experimental time to one day, so this system significantly reduces the time of practical experiments in the laboratory, simplifies the experimental process and saves the cost of reagents, showing significant advantages.

[0047] Methylation level analysis scheme: For samples subjected to enzymatic digestion, non-enzymatically digested samples with different methylation levels are respectively used as controls to calculate the methylation levels of different samples. The methylation level calculation method: each site is normalized relative to the gene serving as an internal standard, i.e., the normalized depth of each CpG site in the enzymatically digested samples / the normalized depth of each CpG site in the control samples.

[0048] Mutation detection assay flow chart. Vardict or GATK software is used to search for variants. If Vardict is used for analysis, Hg19 is used as the BED file to display hg19 coordinates. If GATK is used for analysis, control samples are used to search for variants.

[0049] The aim of this application is to develop a technical scheme that enables the joint detection of mutation and methylation in the same hybrid capture system, which shortens the detection time, simplifies the experimental method, increases the sample utilization rate, reduces the cost of reagents, and has broad market application prospects compared with standard detection technical schemes.

[0050] General stages of the experiment

[0051] Stage 1. Sample fragmentation

[0052] DNA sample fragmentation can be performed according to the laboratory's available options. For fragmentation of samples with an average fragment length of 200-250 bp, it is recommended to use the Covaris™ series ultrasonic DNA homogenizer. gDNA or FFPE DNA were processed for stage 2 after degradation. cfDNA samples were processed directly for stage 2.

[0053] Stage 2. End repair and A-tail attachment

[0054] 1. The end repair and A-tailing buffer was thawed at room temperature, mixed thoroughly, and placed on ice for further use.

[0055] 2. The end repair and A-tailing enzyme was placed on ice to naturally defrost, mixed thoroughly, and immediately centrifuged for further use.

[0056] 3. The reaction system was prepared in a 0.2ml PCR tube on ice according to the table below.

[0057] Note: If the DNA volume is less than 20 µl, add nuclease-free water to make it 20 µl.

[0058] 4. Thorough mixing and instant centrifugation resulted in all reaction liquids being at the bottom of the PCR tube.

[0059] 5. The following reaction programs were run in the PCR analyzer (cycling program I). When the temperature stabilized at 20°C, the PCR tube was placed in the PCR analyzer:

[0060] Note: Do not use the thermal lid during the 20°C program, and set the thermal lid temperature to 70°C during the 65°C program.

[0061] Stage 3. Adapter ligation

[0062] 1. The ligation buffer was thawed at room temperature, mixed thoroughly and placed on ice for further use.

[0063] Note: The ligation buffer has high viscosity, so to ensure accurate volume when using a pipette, it should be pipetted and released slowly and evenly.

[0064] 2. DNA ligase was placed on ice to naturally defrost, mixed thoroughly, and immediately centrifuged for further use.

[0065] 3. The PCR tube for step 2 was removed from the PCR analyzer and placed on plates on ice. The reaction system was prepared according to the table below.

[0066] Step 4. Purification of the ligation product

[0067] Purification and extraction were carried out using 0.5× particles. The purification process was as follows.

[0068] 1. In step 3, 20 µl of NadPrep® SP particles were added to the ligation reaction product. The mixture was thoroughly mixed. Incubation was carried out at 20-25°C for 10 minutes.

[0069] 2. The PCR tube was immediately centrifuged and then placed on a magnetic stand for 5 minutes until the liquid became completely clear. The supernatant was removed using a pipette and discarded.

[0070] Note: The liquid must be completely clear. Different brands of magnetic stands require adjustments in placement time.

[0071] 3. Slowly add 150 µl of 80% ethanol down the side of the PCR tube. Note: Do not disturb the magnetic particles. Allow to stand for 30 seconds. The supernatant was removed with a pipette and discarded.

[0072] 4. Step 3 was repeated once.

[0073] 5. The PCR tube was immediately centrifuged and then placed on a magnetic stand. A small amount of residual ethanol was removed using a 10 µl pipette tip. Note: Avoid drawing magnetic particles into the pipette.

[0074] 6. Open the PCR tube cap and incubate at 20-25°C for approximately 2-3 minutes until the ethanol has completely evaporated.

[0075] 7. Transfer to PCR tube. Add 44 µl of nuclease-free water to the PCR tubes. The magnetic particles were thoroughly resuspended using a pipette. Incubation was performed at 25°C for 2 minutes.

[0076] 8. The PCR tube was immediately centrifuged and then placed on a magnetic stand for 2 minutes until the liquid became completely clear. The supernatant was carefully transferred using a pipette into a new 0.2 ml PCR tube.

[0077] Stage 5. Enzymatic digestion with methylation-sensitive restriction endonuclease

[0078] 1. MeEnzyme and MeBuffer were placed on ice to naturally defrost (NEB restriction endonuclease), mixed thoroughly and immediately centrifuged for further use.

[0079] 2. The reaction system was prepared in a 0.2 ml PCR tube on ice according to the table below. When using a methylation-sensitive restriction endonuclease, the system looked like this.

[0080] 3. The following reaction programs were initiated in the PCR analyzer. When the temperature stabilized at 37°C, the PCR tube was placed in the PCR analyzer: 37°C for 1 hour; 80°C for 20 minutes.

[0081] Stage 6. Purification of the enzymatic cleavage product

[0082] Purification and extraction were performed using 1× particles. For the purification process, see step 4. Elution was performed using 20 µL of nuclease-free water.

[0083] Stage 7. PCR amplification of the enzymatic cleavage product

[0084] 1. 2× HiFi PCR Master Mix and NadPrep® Universal UDI-Index Primer Mix were placed on ice to naturally defrost, mixed thoroughly and immediately centrifuged.

[0085] 2. The PCR amplification mixture was prepared in a 0.2ml PCR tube on ice according to the table below.

[0086] 3. The PCR tube was placed into the PCR analyzer and the following programs were run.

[0087] Step 8. Purification of the amplification product

[0088] Purification and extraction were performed using 1× particles. For the purification process, see step 4. Elution was performed using 20 µL of nuclease-free water.

[0089] Stage 9. Library hybridization

[0090] 1. The hybridization reaction system was prepared according to the table below.

[0091] 2. The mixed hybridization reaction liquid was vortexed and mixed thoroughly for more than 10 seconds. After immediate centrifugation, the mixed reaction liquid was collected at the bottom of the PCR tube (avoiding the formation of bubbles).

[0092] 3. The PCR tube was placed into the PCR analyzer and the following reaction programs were run: 98°C for 2 minutes; 60°C for 1 hour.

[0093] Step 10. Library capture and elution

[0094] Magnetic Particle Washing

[0095] 1. The particles with immobilized streptavidin were mixed on a vortex mixer for 15 seconds to ensure thorough mixing.

[0096] 2. n×25 μL of the streptavidin-immobilized particles were collected and subjected to washing with stirring in a 0.2 mL centrifuge tube (n was the number of captured libraries when n<5).

[0097] Note: If n>5, washing with stirring should be carried out in several tubes.

[0098] 3. The particles with immobilized streptavidin were placed on a magnetic stand and incubated for approximately 2 minutes until the liquid became completely clear. The supernatant was discarded using a pipette.

[0099] 4. The centrifuge tube was transferred from the magnetic stand. 100 µL of pre-warmed Wash Buffer A was added. Thorough mixing was performed by gentle pipetting more than 10 times.

[00100] 5. The centrifuge tube was placed on the magnetic stand to stand for approximately 2 minutes until the liquid became completely clear. The supernatant was discarded using a pipette.

[00101] 6. Step 4 and step 5 were repeated once.

[00102] 7. The centrifuge tube was immediately centrifuged. All the Wash Buffer A at the bottom of the tube was removed using a 10 µL tip. 8. n×8 µl µHyb #1 was used to resuspend the streptavidin-immobilized particles. Thorough mixing by gentle pipetting was performed more than 10 times.

[00103] Magnetic particle capture

[00104] 1. After the hybridization reaction had been running for 1 hour, this step was followed by attachment by capture.The PCR analyzer was maintained in working condition.

[00105] 2. With the PCR tube placed in the PCR analyzer, 8 μl of the resuspended streptavidin-immobilized particles were collected for immediate addition to each hybridization reaction liquid. Thorough mixing by gentle pipetting was performed more than 10 times.

[00106] 3. Incubation was performed at 60°C for 10 minutes.

[00107] 4. After incubation, the PCR tube was removed from the PCR analyzer and placed on a magnetic stand to stand for 2 minutes, until the liquid became completely transparent. The supernatant was discarded using a pipette (the supernatant was removed as completely as possible).

[00108] Elution

[00109] 1. The PCR tube from the previous step was removed from the magnetic stand. 150 µl of pre-warmed wash buffer A was added. Thorough mixing by gentle pipetting was performed more than 10 times (avoiding the formation of bubbles).

[00110] 2. The PCR tube was placed on a magnetic stand to incubate for 2 minutes until the liquid became completely clear. The supernatant was discarded using a pipette. 3. The PCR tube was transferred from the magnetic stand. 100 µl of pre-warmed Wash Buffer A was added. Thorough mixing by gentle pipetting was performed more than 10 times. The reaction liquid was transferred to a new PCR tube.

[00111] Note: At this stage, bubble formation is avoided as much as possible, since bubbles in contact with the tube lid may lead to a decrease in the hit level.

[00112] 3. The PCR tube was placed in the PCR analyzer. Incubation was carried out at 60 °C for 3 minutes.

[00113] 4. The PCR tube was placed in the PCR analyzer. Incubation was carried out at 60°C for 3 minutes.

[00114] 5.After incubation, the PCR tube was removed from the PCR analyzer and placed on a magnetic stand to incubate for 2 minutes, until the liquid became completely clear. The supernatant was discarded using a pipette.

[00115] 6. 150 µl of wash buffer B stored at room temperature was added to the PCR tube. Thorough mixing by gentle pipetting was performed more than 10 times.

[00116] 7. The PCR tube was placed on a magnetic stand to incubate for 2 minutes, until the liquid became completely clear. The supernatant was discarded using a pipette.

[00117] 8. The PCR tube was immediately centrifuged and then placed on a magnetic stand. A small amount of residual liquid was removed using a 10 µl tip. Note. Avoid drawing magnetic particles into the pipette dispenser.

[00118] 9. Add 22.5 µl of nuclease-free water.Gentle pipetting washes were performed more than 10 times to resuspend the magnetic particles.

[00119] Step 11. PCR Amplification of the Hybrid Capture Library

[00120] 1. 2× HiFi PCR Master Mix and NadPrep® Universal UDI-Index Primer Mix were placed on ice to naturally defrost, mixed thoroughly, and immediately centrifuged.

[00121] 2. The PCR amplification mix was prepared in a 0.2 mL PCR tube on ice according to the table below.

[00122] 3. The PCR tube was placed in the PCR analyzer and the following programs were run.

[00123] Step 12. Purification of the Amplification Product

[00124] Purification and recovery were performed using 1× beads. For the purification process, see Step 4. Elution was performed using 20 µl of nuclease-free water.

[00125] Example: Co-Detection of Mutation and Methylation Using Standards

[00126] OGTM800 standards were used to prepare two libraries. The library preparation method is briefly described below. 40 ng of the sonicated standard was collected for end repair, A-tailing, and adapter ligation; the adapter ligation product was divided into two groups: one group was digested with the methylation-sensitive restriction endonuclease Hha I, and the other group was not digested to serve as a control. Both product groups were respectively subjected to PCR amplified with the addition of the index sequence, as shown in Fig. 4A.The amplified products were subjected to hybrid capture using the μCaler Hybrid Capture System with simultaneous capture using three panels, i.e., the 10K MePanel panel, the Normalized Panel panel, and the OGTM800 panel. The 10 KB probe spanned a methylation-sensitive restriction endonuclease cleavage site. The Normalized Panel was used for normalization. The OGTM800 panel was used for mutation detection analysis. For a detailed description of the experimental steps, see the experimental method. For a schematic of the probe design, see Chinese Patent Publication CN 116083423 A, “Probe for Targeted Enrichment of Nucleic Acid.”

[00127] The sequences of the MePanel probe are as follows.

[00128] The normalized panel probe sequences are as follows.

[00129] Analysis of Results The OGTM-CK experimental group serves as a control and is not digested with a methylation-sensitive restriction endonuclease. Samples in this group can be used for mutation detection analysis. The OGTM-MSRE group is a digestion group and is digested with a methylation-sensitive restriction endonuclease. The OGTM800 panel developed in this method does not provide a methylation-sensitive restriction endonuclease cleavage site. Samples in this group can be used for methylation analysis and mutation detection. Figure 4B shows the hit rate and mappability, with mappability exceeding 99%. Figure 4C shows the depth of coverage of the different experimental groups, where 0.2× average coverage is >95% and 0.5× average coverage is >70%. Figure5 shows the sequencing depths and the corresponding methylation levels of CpG sites associated with methylation-sensitive restriction endonuclease in the target regions of different libraries, where the methylation level calculation method is: digestion depth / control depth; (A) The average CpG methylation level of the two experimental digestion groups; (B) The methylation level corresponding to each CpG site. Fig. 6 shows the results of the agreement analysis between the detected mutation rates and the theoretical mutation rates, where the designed probes cover 15 mutation sites, including 11 mutation sites, 2 fusion sites, and 2 copy number variations (base insertions and deletions), including sites where variation in mutation detection is commonly observed in cancers. The corresponding mutation rates are between 1% and 7%. The OGTM-CK experimental group does not undergo digestion.The OGTM-MSRE experimental group is subjected to enzymatic digestion using a methylation-sensitive restriction endonuclease. All sites of the mutation panel coverage selected in this example do not contain an endonuclease recognition site, so the detection results of both the OGTM-CK and OGTM-MSRE groups can reflect the true mutation levels of the samples. The results of the analysis of the agreement between the detected mutation frequencies and the theoretical mutation frequencies show that all results detected in the different experimental groups are consistent with theoretical expectations. OGTM-CK represents the mutation detection conditions of the control group in Fig. 2A. If the mutation detection site contains a methylation-sensitive restriction endonuclease cleavage site, it is recommended to use this experimental design scheme. OGTM-MSRE represents the mutation detection results in Fig. 2B.If the mutation detection site does not contain a methylation-sensitive restriction endonuclease cleavage site, it is recommended to use this experimental design scheme.---> <?xml version="1.0" encoding="ISO-8859-1"?><!DOCTYPE ST26SequenceListing SYSTEM "ST26SequenceListing_V1_3.dtd"PUBLIC "- / / WIPO / / DTD Sequence Listing 1.3 / / EN"> . <st26sequencelisting productiondate="2025-07-24" softwareversion="2.3.0" softwarename="WIPO Sequence" filename="PN311153.xml" dtdversion="V1_3"> <applicantfilereference>PN311153< / applicantfilereference> <earliestpriorityapplicationidentification> <ipofficecode> CN< / ipofficecode> <applicationnumbertext> 202310782691.4< / applicationnumbertext> <filingdate> 2023-06-29< / filingdate> < / earliestpriorityapplicationidentification> <applicantname languagecode="ru"> NANODIGMBIO (NANJING) BIOTECHNOLOGYCO., LTD.< / applicantname> <applicantnamelatin> NANODIGMBIO (NANJING) BIOTECHNOLOGY CO.,LTD.< / applicantnamelatin> <inventiontitle languagecode="en"> Method for Detecting Methylation and Mutation States of DNA Sample< / inventiontitle> <inventiontitle languagecode="ru"> A METHOD FOR DETECTING METHYLATION STATES AND MUTATIONS IN A DNA SAMPLE< / inventiontitle> <sequencetotalquantity> 219< / sequencetotalquantity> <sequencedata sequenceidnumber="1"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier><INSDQualifier_name> mol_type< / INSDQualifier_name><INSDQualifier_value> other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q3"><INSDQualifier_name> note< / INSDQualifier_name><INSDQualifier_value> 5' biotin< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q4"><INSDQualifier_name> organism< / INSDQualifier_name><INSDQualifier_value> synthetic construct< / INSDQualifier_value>< / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgggagagcggtgggaagtattcgcctccgttcctcacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="2"> <insdseq><INSDSeq_length>50< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..50< / INSDFeature_location><INSDFeature_quals> <insdqualifier><INSDQualifier_name> mol_type< / INSDQualifier_name><INSDQualifier_value> other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q6"><INSDQualifier_name> note< / INSDQualifier_name><INSDQualifier_value> 5' biotin< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q7"><INSDQualifier_name> organism< / INSDQualifier_name><INSDQualifier_value> synthetic construct< / INSDQualifier_value>< / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgacaggatgcgagcgccctttggagaagtcgcgccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="3"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier><INSDQualifier_name> mol_type< / INSDQualifier_name><INSDQualifier_value> other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q9"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q10"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggcggaaacgctaactccccggccaagtgcaaatgcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="4"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q12"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q13"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggcacacacgagggcccgtcgcgccccccgccctgccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="5"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q15"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q16"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggcctcgccctccacgtccctgcacccccaagtcgcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="6"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q18"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q19"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgtaagaacccagtccccgatcggtttcctctacgccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="7"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q21"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q22"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtcgggagggagggggggaaaggggacgaggggcggacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="8"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q24"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q25"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgggggggtttggaagcgcactgccacttgcaaagtccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="9"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q27"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q28"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgacccgagcaatcacagccggagcgcgattcgaatgcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="10"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q30"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q31"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgtgaaccgaagacctcgtaggcgggcctcggggggcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="11"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q34"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q35"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggatgccgttggcggcggccagcgccagcccctcggcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="12"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q38"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q39"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggtgccgtagagcagctgcagctcgcgcgcctcgtcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="13"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q41"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q42"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgggctcaggtcgccgcagcccgggagcctccccgccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="14"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q44"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q45"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggccccaaggcacgcgcggcacagccatgaacacccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="15"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q47"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q48"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggatgccaaggagtatctggcccggagggaaatcccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="16"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q50"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q51"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggagtcccgcgcgctctccgtgcgccccggccggccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="17"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q53"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q54"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggcggcggctgccgcgcacaggcttccgactccagccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="18"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q56"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q57"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgcggcccgccactgagcatgcccagcacgccggcccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="19"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q59"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q60"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtcggagcggaggggacagcggggatcgtgagctccggccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="20"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q62"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q63"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggggcgagcgggtgcgtctgccgcagagtcggcacccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="21"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q65"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q66"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtcgaaggacatggaggtaaaggaccccggagggagacgcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="22"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q68"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q69"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggcacgtgcggcgctcagcttaggctctcggaggcacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="23"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q71"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q72"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgtgagttggaaatcccgacggaaagcacccacaagccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="24"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q74"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q75"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgcactctgcgctggcccacccgcgtgcacgcccaccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="25"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q77"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q78"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtcggcggagctaagcaggggtgtttggaaggcgcccgcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="26"> <insdseq><INSDSeq_length>50< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..50< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q80"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q81"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggctgtttgactgcgtggcttcacttggcccgcgcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="27"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q83"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q84"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgatcagcctctctggcgggggattctccccggactccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="28"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q86"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q87"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgggcgggcggggagccaggcccgagctgcgttctgccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="29"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q89"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q90"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgagccattggtgggcgccgcgctctgcactgagcacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="30"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q92"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q93"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgtcgcgccccgccggcccctagccgcagccgcagcccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="31"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q95"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q96"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgggcaggcggcaggacggggatcccaggctgcggggcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="32"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q98"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q99"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtcgactccgcggcagagccgctgggacctgacccgggacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="33"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q101"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q102"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggccttcgtccccgcgcgcacctccccgggtcgggccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="34"> <insdseq><INSDSeq_length>50< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..50< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q104"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q105"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgaaggggggctggtggaatctggcggtccccagccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="35"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q107"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q108"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgcgtgtcccgggtcggtgcgctcggcgcacccgtgcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="36"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q110"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q111"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggacagtgcccggcgtctgctcccacccgcccgccccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="37"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q113"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q114"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggaacttgccggtggtggggtcgtagtggtttccgacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="38"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q116"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q117"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgttggtgaccacgtcgtcgaacttgagcacctcgtacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="39"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q119"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q120"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgttcatgctgccgcttgaggccggcgtagaaggcgcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="40"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q122"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q123"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgggcggcccgggcaccgcgagccggccgagctccacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="41"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q125"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q126"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgggagctacgtgactacgtccacccgcacctacagcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="42"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q128"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q129"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgtgggcagcgcgctgcgccccagcaccagccgcagccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="43"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q131"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q132"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgggcgccgggcccgcgatcggagccggaggcaccgccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="44"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q134"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q135"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgagcggaaagagggctccgcggcggccaggctcagccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="45"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q136"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q137"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgtgccgggaaaatgctggatttaactcccagtctgccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="46"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q139"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q140"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgtcccggggatgccagactcctggggacgctgggacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="47"> <insdseq><INSDSeq_length>50< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..50< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q142"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q143"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggcggcgcggcgggacacgcaggactcccgcctccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="48"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q145"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q146"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggcccggaattcgttgagacggaatctcagcggatccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="49"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q148"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q149"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgtggggagaagcgctgcggaaaggggcgactccgacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="50"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q151"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q152"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgagatggccctgtcccggcgccccaggtcgtcgcgccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="51"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q154"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q155"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggcagctgcggtagtcactgcgcctccccgcccccacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="52"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q157"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q158"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgctggatgccccccttccctctcccggccagactccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="53"> <insdseq><INSDSeq_length>50< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..50< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q160"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q161"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgcggtgctgtgtgcctggccggagcgttttgaaacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="54"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q163"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q164"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgtccggggccgctcggcggcgctgcgattggccgcgcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="55"> <insdseq><INSDSeq_length>50< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..50< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q166"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q167"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggggtaacctctatgcaaatgaggccgcgccgcccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="56"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q169"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q170"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggcgggctcggcccggtccctccgctggggggttacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="57"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q172"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q173"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgagccggccggcagcgagatacccagaggcccgttcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="58"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q175"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q176"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgcaccgcgttgccatagcaacggcctggccggctgcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="59"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q178"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q179"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggaggtgccggctgcacaacggcgcccacgtgaccccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="60"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q181"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q182"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggtgcagccccaggcgcgcccaagggcagctcgcccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="61"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q184"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q185"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtcggagaaggacctaaaaccccgtctggggcacagaggcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="62"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q187"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q188"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtcgacatctgggaaaccgaggcgcagagcgggacggccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="63"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q190"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q191"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtcgtgggcctggcgggaagtcgcgcaaggaggccggcacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="64"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q193"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q194"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggggcgcacccggcccaccctgtcccttccaggccgcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="65"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q196"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q197"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggctgcagcacaggctgcagggggcgggcagcgccgcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="66"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q199"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q200"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtcgtctagcggggcaggcgagcggagcagcagcgttccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="67"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q202"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q203"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgagccccggccgcggcccctgagaggcgcgcactcgcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="68"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q205"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q206"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggcctccctgtgccagccggctgtcgacccaggttacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="69"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q208"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q209"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtcgaagcccgaggtcggggcgttaccccaaagggccctccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="70"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q211"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q212"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggcttcccctccgagggcagagaggcgtccgcgccccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="71"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q214"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q215"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgggggctgcggggtccgtggaacctttccgggaggccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="72"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q217"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q218"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggccgcgggctgctgggcgcggaggctggagccgcgcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="73"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q220"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q221"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgaggttcggctgctgaagcggccactgcctcccagacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="74"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q222"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q223"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtcggatcgatggagagaaggcgggcaagacgccgggaacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="75"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q225"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q226"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggcattcctcctcaaccgagtgccacaaccgccctccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="76"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q228"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q229"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggaagtgccccggggcttcgagcatcacctcgcggcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="77"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q231"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q232"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtcgagctgagaccggcggccgacggccagccctcagggcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="78"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q234"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q235"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgggtcacaagtcagcgcccaagcaagtcaagcgaccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="79"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q237"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q238"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggctcgtcttcgcccgaactgatgcgctgcaaacgcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="80"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q240"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q241"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgggctcaacttcagcggctttggctacagcctgccgcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="81"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q243"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q244"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggagcgcaaccgcgtcaagttggtcaacctgggctcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="82"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q246"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q247"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgcacccttcgggagcacgtccccaacggcgcggcccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="83"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q249"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q250"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtcgaagaagatgagtaaggtggagacactgcgctcggcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="84"> <insdseq><INSDSeq_length>50< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..50< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q252"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q253"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgtggccctggttgggtccgcccgcagcgagggcgcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="85"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q255"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q256"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtcgagacctgcgaagaagttcggaaacttttccagtggcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="86"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q258"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q259"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggctgctgggagctgtcagggggctgccggattcgccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="87"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q261"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q262"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtcggccgaggctgggagcgcggtgatggccggtccccgcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="88"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q264"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q265"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtcgagcagagggcagcggcggcgggaccggggactctgcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="89"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q267"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q268"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtcgcaatgggtaaagccaggaggaagcccgtgcctggcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="90"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q270"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q271"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtcgaggcgggcaggagggggaggagtagggaggcgagagacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="91"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q273"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q274"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtcggcgggcgggaggcgccacggcctctcagacgctggcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="92"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q276"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q277"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtcgccgcagccgagaaggccagagaagaaagttcccgggcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="93"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q279"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q280"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtcggcagtaagcgagtaagcgagccctggagaccgggcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="94"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q282"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q283"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgctgggtgtgcggtcggctcagtgccacgcggtgacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="95"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q285"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q286"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgtaggcatggttatcaacccacgccaaggcggttgcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="96"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q288"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q289"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggctgcctcgccccggagcccggaggaggggagccgcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="97"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q291"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q292"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtcgacccggggaagaagcggagcgccgatctggcccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="98"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q294"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q295"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgtgcgttgcgcgctccaaccctctgcttggccgcccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="99"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q297"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q298"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgacaaatccccgaggcggggagactttcagggcatccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="100"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q300"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q301"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggatgctgaagcctcgcggtccccattcccaagccccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="101"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q303"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q304"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgccgtgcgccgcctgcgcgtggcgcagttaatttgcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="102"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q306"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q307"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtcggtcttgaggtggggagggagaaatgggaagaggcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="103"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q309"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q310"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtcgagtagcggttttagcccgctctgcggctgcgaggcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="104"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q312"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q313"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtcgagatccgagattaacctctcccgcgataggtgaagcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="105"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q315"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q316"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgttggggcgacggggccaggcgcgggggacggctgcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="106"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q318"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q319"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtcgggctgggagggcgcgcggaggagacaccgctgaggcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="107"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q321"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q322"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgacgccgctgagggcgccacgcggggggcgcggctgcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="108"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q324"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q325"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgagtgcgcacttgaggcgcgggacgcaccggctgtgcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="109"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q327"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q328"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgtcccaggggctgagactcgcgccccgccccgcagccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="110"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q330"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q331"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgtcggctcgccgggccgggcccctctccggaacctccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="111"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q333"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q334"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggcggctggaggacgcgctgctgcggatgcgcgaggcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="112"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q335"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q336"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtcgtacgggatacaggccgaggacggcaggtccgtgccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="113"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q338"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q339"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgggggatggcgcgctgaccccatacccgctgccgtccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="114"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q341"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q342"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgcggctgcggggccgccgccgagcgagggcgaggacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="115"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q344"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q345"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggcaccgtgcgcttcgcccgcaaaggcgccctccggcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="116"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q347"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q348"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtcggaagaacgtgcatgaggtcaagaaccacaaattcacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="117"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q350"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q351"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggatggccgcgccgccccgggggagccggagccgacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="118"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q353"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q354"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgtggccgccgcccgcgcgcactcaccaagcccgctgcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="119"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q356"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q357"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgtcctcctcgcacgagtaccagatgccggtgtggacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="120"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q358"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q359"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtcgtccctgggccgggactggagcgcaaacggttgggacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="121"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q361"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q362"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgtgtcggtgtctgcccgcccgtctgtgcgtctgtccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="122"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q364"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q365"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtcgtgtcggtgtgagcgtctgaggcgatgggggaagacacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="123"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q367"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q368"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtcggggcgcttctagtcggacaaaatgcagccgagaacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="124"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q370"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q371"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgcgctcgttctgtgcgttctcctgtcccaggtaggcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="125"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q373"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q374"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgagaggggctgccgggcgcgctctgcgccccgtttccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="126"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q376"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q377"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgtgggagcagggtgtccgaagcgcgggtaccgatccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="127"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q379"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q380"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgggcagcgcggggcacgcggcgcgcgggcgagtctgcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="128"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q382"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q383"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgtgcgcgcgcggacgccttggttcccggagcccagcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="129"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q385"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q386"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgccaccttctcagtccgggacgcgagacgggaacgcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="130"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q387"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q388"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggccctggcgccccaacgctgcaccaccgtgggtccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="131"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q390"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q391"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggagccgtgctcgcagtcgctgagatcctcggtgcgcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="132"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q392"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q393"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggcgtctctcgccgtcccctgggcgcgggccaggcgcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="133"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q395"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q396"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtcggagggggggcgctccggtcgtgtgcccaggaccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="134"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q398"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q399"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgccccagcggccactcgggccccagccccccaggccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="135"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q401"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q402"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgtggagatttagagcccgggaatgaggcgccgtgtccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="136"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q404"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q405"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtcgtaggaatctcgcgcccgggagcgctgagggaccgcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="137"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q407"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q408"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggagccgggtggggagcgggcctcggggcgcccggccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="138"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q410"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q411"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggcagggccggaaggaggcccctgccccgtccaaggcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="139"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q413"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q414"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggcaccagctgccccgccgcccgcccggacccagcccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="140"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q416"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q417"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggcctcattgctgacgagaccccgccctgctactcccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="141"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q419"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q420"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtcgggccggagaaggcgccgctggggccgcccggaggacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="142"> <insdseq><INSDSeq_length>50< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..50< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q422"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q423"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgcctctgcacgggcccgtggagacgcttcctgcgcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="143"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q425"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q426"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgaaccttctggaatccaccgcccccctttccgcccgcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="144"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q428"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q429"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgcgcgcccgcccccaggagggcctccgcgagccggcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="145"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q431"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q432"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggcacaccccgaggcggtcccggctgcacaacttggcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="146"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q434"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q435"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggagttgctccgtttcctcattttgggggcgaagacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="147"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q437"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q438"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggccggcggcggccagtagcggcgccgtgtgcagggcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="148"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q440"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q441"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgagcgccgcctgcgccgcgttcagcacgaagagctccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="149"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q443"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q444"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgtccagctcaggcgcagcagcgccacctggtcggcccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="150"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q445"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q446"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgggcgcccgcattggtgctgacataatttcctgacccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="151"> <insdseq><INSDSeq_length>53< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..53< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q448"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q449"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgtgacccgtattgtctcgcgattaaaggtaaaaaacgcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="152"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q451"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q452"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggctttttcatcccactggggtaaaacgcccttttacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="153"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q453"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q454"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgagtggggccgcggggcctgctgggaggtgttgtcccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="154"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q456"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q457"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgggaaacgtcgctggcgcggagggatggttcggcgccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="155"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q458"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q459"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgtaggcgtctgtcacagacctatctgcgggtcgcccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="156"> <insdseq><INSDSeq_length>50< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..50< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q461"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q462"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgccagcgccatagcccttaggactatcggtcacacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="157"> <insdseq><INSDSeq_length>50< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..50< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q464"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q465"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcctcgcgctcctgctccggctcctccatcttggcccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="158"> <insdseq><INSDSeq_length>50< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..50< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q467"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q468"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggagcagagcgaggtgtgtgcctccttaccgcctcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="159"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q470"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q471"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgtgcactggacgtccttccccagcagccagttgagcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="160"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q473"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q474"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcagacggaggctggtggtgcagcaggcaggcaagaccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="161"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q475"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q476"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgatgccggggactacagctgcgaggccaggggccacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="162"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q478"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q479"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgcacagcgttgtgcatccaggtctagcgtgtcttccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="163"> <insdseq><INSDSeq_length>55< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..55< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q481"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q482"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcctgtcttctgtggatgaggtatatgaatttatcccaaagcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="164"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q483"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q484"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtccgagacctcccagagcccgtggttccctggagccacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="165"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q486"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q487"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtctacgaagggttcctgctctgtgggcagctcacgaacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="166"> <insdseq><INSDSeq_length>56< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..56< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q489"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q490"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtccaataatgcacatggagaaagttcacctacaataaagaggcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="167"> <insdseq><INSDSeq_length>55< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..55< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q492"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q493"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtctgtattggtaacattttatatcgaattcctgttctatgtcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="168"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q495"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q496"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcagatgtcccctccctgtccgtccccgcacctggagcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="169"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q498"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q499"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtctggtggtgcatgcccgaacccagcacttccttgaacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="170"> <insdseq><INSDSeq_length>50< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..50< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q501"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q502"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtccaactggccaacctagagcccccctggtaaaagccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="171"> <insdseq><INSDSeq_length>55< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..55< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q504"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q505"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtctcttggtggaatagatgttaattagtttttttattactccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="172"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q507"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q508"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggaaacaattctgtgttcaccctcaccctgcaggccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="173"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q510"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q511"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggcctctcagccatcaagacaccgtatcctacctccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="174"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q513"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q514"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcacgtgctgagcgcacgcacgtggcgcctgctcacccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="175"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q515"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q516"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgatgctgctccagcgcccgcgcgagttgcggatcgcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="176"> <insdseq><INSDSeq_length>56< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..56< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q518"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q519"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgtagttgacaaatacatttagtgatgtcttacttttttggcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="177"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q521"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q522"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcatctcctaccagtgtatccttcacgacagacgcaccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="178"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q524"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q525"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtccgagcgatgatgacaccaaatccatgtgtccaccccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="179"> <insdseq><INSDSeq_length>50< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..50< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q527"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q528"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgggacccaggagggcacagccaaggaatgagccccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="180"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q530"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q531"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtcctcctggtcagcagcctcttagagaacctgctggacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="181"> <insdseq><INSDSeq_length>52< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..52< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q533"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q534"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtctatagaaccatcatcatgcaagatgagagcaaggagcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="182"> <insdseq><INSDSeq_length>56< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..56< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q536"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q537"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtcgaacagtaaacagtggttctgactggtgaaatgatagttacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="183"> <insdseq><INSDSeq_length>55< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..55< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q539"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q540"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgttgaaaggaccagcactttatttattgctttagccattcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="184"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q542"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q543"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcagcctgcacgggagtcagaggcactcagcaatgcccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="185"> <insdseq><INSDSeq_length>50< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..50< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q545"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q546"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtctggctctgttacgaacggctgaaatcaaaacccccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="186"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q547"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q548"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtctcaccgtgttctccagaccatgcatgttcctccgccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="187"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q550"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q551"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcaccgccccttcagggatggggctgtaggagtcaagcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="188"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q553"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q554"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtctctcggtgtctccccaggtgcaagtgcaacctgcacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="189"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q556"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q557"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgccaacctgtgctccatgcgcgagggcagcctgcacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="190"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q559"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q560"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcaagcccactcttctactccccgaacgtgccttccccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="191"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q562"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q563"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcctctgacggcgacagtagctccgtggacagcgatgcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="192"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q565"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q566"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtcctggaggagcacggaaaagacctggaaatcatgcacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="193"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q568"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q569"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcatcctcaccagggtgaatgacagagttgccaggcacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="194"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q571"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q572"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtcgcataggagtaggccagccagcggaacacgtggtccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="195"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q574"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q575"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtctcgggggtgggggtgtgttcctgcgtcttccagggcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="196"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q577"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q578"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtccgggaatgccgagtaagtgttaattttatggtccccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="197"> <insdseq><INSDSeq_length>56< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..56< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q580"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q581"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcatcacttctggaaaatgaatagatagatgtgtgggctttacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="198"> <insdseq><INSDSeq_length>54< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..54< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q583"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q584"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgtgcctcaatgatcttttttccccaccttcatttttctcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="199"> <insdseq><INSDSeq_length>50< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..50< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q586"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q587"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtccttcagcggcatctgctagctcgtctacccctgtcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="200"> <insdseq><INSDSeq_length>54< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..54< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q589"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q590"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtccatgttttagggaactctgccctataaacactcatagacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="201"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q592"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q593"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcggtttctctctatgttgcagtccctctgtcgtgaacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="202"> <insdseq><INSDSeq_length>50< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..50< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q595"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q596"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtccggcattcctgtggtagtggcctgagaacacgaccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="203"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q598"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q599"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtctgacacctgcagagaagggaaaaagtcattaggggcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="204"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q601"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q602"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcacatgtccctgcgtcacaggcacctgcagagcccccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="205"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q603"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q604"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtctgtgtaaggcgttccggcacgtcaaggtggacacacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="206"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q606"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q607"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtctgaagatacaaacaaccacagcagcctgcacttaccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="207"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q609"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q610"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcgtgtggtcatcttcttcttccccttctagcacgaccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="208"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q612"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q613"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcacgttgcatcgaccctctccactccgcggaagtagcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="209"> <insdseq><INSDSeq_length>50< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..50< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q615"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q616"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtccgacaccgtctgatgtgttgcgcaggtggtggcacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="210"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q618"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q619"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtccattgggatggagggtggggaaggagtcaccctggcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="211"> <insdseq><INSDSeq_length>50< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..50< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q621"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q622"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtccctcctccgcctgacttacttgatcctaaagtcacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="212"> <insdseq><INSDSeq_length>50< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..50< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q624"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q625"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcctcccagggctccttggcctcctccgcggtgacccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="213"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q627"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q628"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtcggagacacagggccgcatgagcctgggcggggtcagcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="214"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q630"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q631"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtccgccctccgaggagggcccgggcggggtgggcgcccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="215"> <insdseq><INSDSeq_length>50< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..50< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q633"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q634"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtccgtcctcaggctccaggctgggaggagagagcgtcaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="216"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSeq_moltype> DNA< / INSDSeq_moltype><INSDSeq_division> PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..51< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value>< / insdqualifier> <insdqualifier id="q636"><INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q637"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence> cgtcggtcgctgggagtggagctgggggtcagaagagcaagcacaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="217"> <insdseq><INSDSeq_length>54< / INSDSeq_length><INSDSeq_moltype>DNA< / INSDSeq_moltype><INSDSeq_division>PAT< / INSDSeq_division><INSDSeq_feature-table> <insdfeature><INSDFeature_key>source< / INSDFeature_key><INSDFeature_location>1..54< / INSDFeature_location><INSDFeature_quals> <insdqualifier> <INSDQualifier_name>mol_type< / INSDQualifier_name><INSDQualifier_value>other DNA< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q638"> <INSDQualifier_name>note< / INSDQualifier_name><INSDQualifier_value>5' biotin< / INSDQualifier_value> < / insdqualifier> <insdqualifier id="q639"> <INSDQualifier_name>organism< / INSDQualifier_name><INSDQualifier_value>synthetic construct< / INSDQualifier_value> < / insdqualifier> < / INSDFeature_quals>< / insdfeature> < / INSDSeq_feature-table><INSDSeq_sequence>cgtcggtcaacgaaattgatcaaacttagactgcttcacctgtctccaccgacg< / INSDSeq_sequence>< / insdseq> < / sequencedata> <sequencedata sequenceidnumber="218"> <insdseq><INSDSeq_length>51< / INSDSeq_length><INSDSe...

Claims

1. A method for the simultaneous detection of the methylation state and mutation state of a DNA molecule in a sample, comprising: 1) adding adapter molecules to both ends of a DNA molecule in a sample to thereby obtain an adapter ligation product sample; and dividing the adapter ligation product sample into two parts: a first adapter ligation product sample and a second adapter ligation product sample; 2) treating a first sample of the adapter ligation product with one or more methylation-sensitive restriction endonucleases such that, if the DNA molecule does not contain methylation at the recognition site of the methylation-sensitive restriction endonuclease, an enzymatic cutting reaction occurs, thereby obtaining a sample of the enzymatic cleavage product; and not treating a second sample of the adapter ligation product with a restriction endonuclease; 3) amplifying a sample of the enzymatic cleavage product and a second sample of the adapter ligation product, respectively, using a primer specific to the adapter molecule and having an index sequence, thereby obtaining an amplification product of a sample of the enzymatic cleavage product and an amplification product of a second sample of the adapter ligation product, respectively; and mixing the amplification product of the sample of the enzymatic cleavage product with the amplification product of the second sample of the adapter ligation product, and obtaining a mixture of amplification products; 4) contacting a probe array comprising one or more mutation capture probes and one or more methylation capture probes with a mixture of amplification products to thereby produce a capture product, wherein the methylation capture probes target a target sequence containing a recognition site in a DNA molecule and the mutation capture probes target a target sequence containing a mutation site in a DNA molecule; and 5) sequencing the capture product; determining the methylation state of the DNA molecule containing the recognition site using the ratio of the sequencing depth of the recognition sites in the amplification product of the sample of the enzymatic cleavage product and the amplification product of the second sample of the adapter ligation product; and determining the mutation state based on the results of sequencing the mutation site; wherein the mutation capture probe and / or the methylation capture probe comprise a target-specific sequence, a first probe-binding sequence located at the 5'-end of the target-specific sequence, and a second probe-binding sequence located at the 3'-end of the target-specific sequence; and wherein the first probe-binding sequence is at least partially complementary to the second probe-binding sequence, so that if two or more mutation capture probes and / or two or more methylation capture probes bind to their target sequence adjacently, the adjacent mutation capture probes and / or adjacent methylation capture probes are capable of binding complementarily via the first probe-binding sequence and the second probe-binding sequence.

2. The method according to claim 1, wherein the first probe binding sequence and the second probe binding sequence have a length of 8-30 nucleotides.

3. The method according to claim 1, wherein the target-specific sequence has a length of 20-80 nucleotides.

4. The method according to any one of paragraphs 1-3, further comprising, before step 1), fragmenting the DNA molecule.

5. The method according to any one of claims 1-4, wherein the methylation-sensitive restriction endonuclease is selected from Hpa I, Hpa II, Hha I, Aci I and any combination thereof.

6. The method according to any one of paragraphs 1-5, wherein the sample comprises ctDNA.

7. The method according to any one of claims 1-6, wherein the methylation state comprises the presence of a methylation level.

8. The method according to any one of claims 1-7, wherein the mutation state comprises the presence of a mutation, wherein the mutation type is selected from insertion, deletion and substitution of a base, a change in the number of chromosomal copies, microsatellite instability and gene fusion.

Images