ชิปช่วยวินิจฉัยอาการแพ้และวิธีการสำหรับผลิตชิปช่วยวินิจฉัยอาการแพ้

Abstract

DEPCT6525 / 08 / 2564 ได้มีการเปิดเผยถึงชิปช่วยวินิจฉัยอาการแพ้และวิธีการสำหรับผลิตชิปช่วยวินิจฉัยอาการแพ้ ตามการประดิษฐ์นี้ได้จัดให้มีวิธีการสำหรับผลิตชิปช่วยวินิจฉัยอาการแพ้โดยที่วิธีการ จะมีส่วนที่เป็นขั้นตอนการสร้างส่วนเคลือบซึ่งเป็นการให้กำเนิดความดันลบซึ่งต่ำกว่าความดันปกติ ภายในหน่วยดูดเพื่อยอมให้สารละลายซึ่งบรรจุสารก่อภูมิต้านทานไว้ภายในแผ่นด้านบนนั้นเคลื่อนที่ ลงข้างล่างอันเป็นการสร้างส่วนเคลือบรูปทรงเส้นหลายส่วนซึ่งสารก่อภูมิต้านทานถูกดูดซับไปยัง ส่วนนั้นบนแผ่นเยื่อ -----------------------------------------------------------DEPCT64 ได้มีการเปิดเผยถึงชิปช่วยวินิจฉัยอาการแพ้และวิธีการสำหรับผลิตชิปช่วยวินิจฉัยอาการแพ้ ตามการประดิษฐ์นี้ได้จัดให้มีวิธีการสำหรับผลิตชิปช่วยวินิจฉัยอาการแพ้โดยที่วิธีการจะมี ส่วนที่เป็นขั้นตอนการสร้างส่วนเคลือบซึ่งเป็นการให้กำเนิดความดันลบซึ่งต่ำกว่าความดันปรกติ ภายในหน่วยดูดเพื่อยอมให้สารละลายซึ่งบรรจุสารก่อภูมิต้านทานไว้ภายในแผ่นด้านบนนั้นเคลื่อนที่ ลงข้างล่างอันเป็นการสร้างส่วนเคลือบรูปทรงเส้นหลายอันซึ่งสารก่อภูมิต้านทานถูกดูดซับไปยัง ส่วนนั้นบนแผ่นเยื่อ -----------------------------------------------------------

Description

Allergy diagnostic chip and method for manufacturing the allergy diagnostic chip

[0001] Cross-citation with related applications

[0002] This application claims the benefit of priority based on Korean Patent Application No. 10-2018-0157440 filed on December 7, 2018, and all contents disclosed in the document of said Korean Patent Application are incorporated herein as part of this specification.

[0003] Technology field

[0004] The present invention relates to an allergy diagnostic chip and a method for manufacturing the allergy diagnostic chip.

[0005] Diagnostic chips for analyzing components within a sample are generally manufactured by coating antigens, etc., onto a membrane-type immobilizer. Subsequently, various biological information can be obtained by reacting the antigen coated on the manufactured diagnostic chip with the sample to be analyzed.

[0006] These diagnostic chips can be broadly classified into dot and line types. Dot-type diagnostic chips refer to chips in which antigens, etc., are coated in a dot shape on a fixed substrate, while line-type diagnostic chips refer to chips in which antigens, etc., are coated in a line shape on a fixed substrate. Among these, dot-type diagnostic chips involve the inconvenience of having to individually coat antigens, etc., onto the fixed substrate in a dot shape. On the other hand, line-type diagnostic chips offer an advantage in terms of productivity because antigens, etc., can be coated onto the fixed substrate in a line shape and then cut into the desired form to be used as strips.

[0007] In such line-type diagnostic chips, the smaller the spacing between the lines formed by the coating, the greater the number of coated lines per unit area, allowing for the acquisition of more diverse information with a single detection. Therefore, in the case of line-type diagnostic chips, high integration is required to maximize the number of coated lines per unit area.

[0008] Therefore, the problem that the present invention aims to solve is to manufacture an allergy diagnostic chip that is highly integrated compared to conventional technology.

[0009] According to one aspect of the present invention for achieving the above objective, a method for manufacturing an allergy diagnostic chip is provided, comprising: a step of preparing an upper plate having a space formed therein for receiving a solution containing an antigen, a suction unit provided at the lower part of the upper plate, and a lower plate provided at the lower part of the upper plate and mounted on the suction unit; a step of providing a membrane having a membrane on the upper surface of the lower plate; and a step of forming a coating part having a plurality of line-shaped coating parts formed on the membrane, wherein the antigen is adsorbed thereon, by forming a negative pressure, which is lower than normal pressure, inside the suction unit to move the solution containing the antigen within the upper plate downward.

[0010] The thickness of the plurality of the above-mentioned coating portions may be 0.2 mm to 0.5 mm.

[0011] In the step of forming the coating portion above, the spacing between the plurality of the coating portions may be 0.5 mm to 0.9 mm.

[0012] The step of providing the membrane above may further include providing a protective plate between the suction unit and the membrane to prevent damage to the membrane caused by the difference between the negative pressure and the atmospheric pressure.

[0013] It may further include a pressurizing step provided between the above membrane provisioning step and the above coating portion forming step, wherein a portion of the upper region of the coating unit is additionally pressed so that the membrane can be closely attached downward.

[0014] According to another aspect of the present invention for achieving the above objective, a method for manufacturing an allergy diagnostic chip is provided, comprising: a step of preparing an upper plate having a space formed therein for receiving a solution containing an antigen, a suction unit provided at the lower part of the upper plate, and a lower plate provided at the lower part of the upper plate and mounted on the suction unit; a step of providing a membrane having a membrane on the upper surface of the lower plate; and a step of forming a coating part by forming a negative pressure, which is lower than normal pressure, inside the suction unit to move the solution containing the antigen within the upper plate downward, thereby forming a plurality of line-shaped coating parts on which the antigen is adsorbed on the membrane, wherein the negative pressure is formed for 7 seconds or more in the step of forming the coating part.

[0015] According to another aspect of the present invention for achieving the above objective, an allergy diagnostic chip is provided comprising: a sheet-shaped membrane; and a plurality of coating portions, which are regions coated with an antigen on the membrane, wherein the coating portions have a constant thickness (t), the plurality of coating portions are spaced apart from each other by a constant interval (d), and the ratio (t / d) of the thickness (t) of the coating portions to the interval (d) between the coating portions is 0.40 to 0.55.

[0016] The thickness (t) of the coating portion may be 0.2 mm to 0.5 mm.

[0017] The spacing (d) between the plurality of coating portions may be 0.5 mm to 0.9 mm.

[0018] According to the present invention, a highly integrated allergy diagnostic chip can be manufactured compared to the prior art.

[0019] FIG. 1 is a perspective view illustrating the structure of a manufacturing apparatus that can be used in the method for manufacturing an allergy diagnostic chip according to the present invention.

[0020] FIG. 2 is a side cross-sectional view illustrating the structure of a manufacturing device that can be used in the method for manufacturing an allergy diagnostic chip according to the present invention.

[0021] FIG. 3 is a plan view illustrating the structure of a suction unit among the manufacturing devices that can be used in the present invention.

[0022] FIG. 4 is a side cross-sectional view illustrating the structure of a suction unit among the manufacturing devices that can be used in the present invention.

[0023] FIG. 5 is a perspective view illustrating the structure of an example of an upper plate among manufacturing devices that can be used in the present invention.

[0024] FIG. 6 is a plan view illustrating the structure of an example of an upper plate among manufacturing devices that can be used in the present invention.

[0025] Figure 7 is a cross-sectional view illustrating the structure of an upper plate cut along line AA of Figure 6.

[0026] FIG. 8 is a perspective view illustrating the structure of another example of an upper plate among manufacturing devices that can be used in the present invention.

[0027] FIG. 9 is a plan view illustrating the structure of another example of an upper plate among manufacturing devices that can be used in the present invention.

[0028] FIG. 10 is a cross-sectional view illustrating the structure of an upper plate cut along the BB line of FIG. 9.

[0029] FIG. 11 is a perspective view illustrating the structure of a lower plate among manufacturing devices that can be used in the present invention.

[0030] FIG. 12 is a side cross-sectional view illustrating the structure of a lower plate cut along the CC line of FIG. 11.

[0031] FIG. 13 is a plan view illustrating the structure of an allergy diagnostic chip manufactured according to the present invention.

[0032] FIG. 14 is a diagram showing the coating state of a diagnostic chip manufactured according to an embodiment and a comparative example of the present invention.

[0033] Hereinafter, with reference to the drawings, a method for manufacturing an allergy diagnostic chip according to the present invention and a manufacturing apparatus that can be used in the manufacturing method will be described.

[0034]

[0035] Chip manufacturing method and chip manufacturing device

[0036] FIG. 1 is a perspective view illustrating the structure of a manufacturing device that can be used in the method for manufacturing an allergy diagnostic chip according to the present invention, and FIG. 2 is a side cross-sectional view illustrating the structure of a manufacturing device that can be used in the method for manufacturing an allergy diagnostic chip according to the present invention.

[0037] As illustrated in FIGS. 1 and 2, an allergy diagnostic chip manufacturing device (10, hereinafter referred to as the "manufacturing device") according to the present invention may include an upper plate (100) capable of receiving a solution containing an antigen. The upper plate (100) may be configured to receive a solution containing an antigen from the outside and discharge it downward.

[0038] A suction unit (200) capable of moving a solution containing antigens contained within the upper plate (100) downward due to a pressure difference may be provided at the lower part of the upper plate (100). As described below, the suction unit (200) according to the present invention may be configured to move external air downward due to the pressure difference between the internal pressure of the suction unit and the external pressure, thereby causing the solution containing antigens within the upper plate (100) to be discharged downward.

[0039] Meanwhile, referring to FIGS. 1 and 2, a lower plate (150) mounted on a suction unit (200) may be provided at the lower part of the upper plate (100).

[0040] Referring to the foregoing description and FIGS. 1 and 2, the method for manufacturing an allergy diagnostic chip according to the present invention may include the step of preparing an upper plate (100) in which a space is formed to accommodate a solution containing an antigen, a suction unit (200) provided at the bottom of the upper plate (100), and a lower plate (150) provided at the bottom of the upper plate (100) and mounted on the suction unit (200). Additionally, the method for manufacturing an allergy diagnostic chip according to the present invention may further include the step of providing a membrane on the upper surface of the lower plate (150).

[0041] FIG. 3 is a plan view illustrating the structure of a suction unit among a manufacturing device that can be used in the present invention, and FIG. 4 is a side cross-sectional view illustrating the structure of a suction unit among a manufacturing device that can be used in the present invention.

[0042] As shown in FIGS. 3 and 4, a space (210) which is a space with an open top can be formed in the suction unit (200), and a lower plate (150) can be mounted on the top of the space (210).

[0043] As described above, the solution containing antigen contained within the upper plate (100) can be discharged downward by a suction unit. Additionally, when the solution containing antigen is discharged downward, a thin membrane is provided on the upper surface of the lower plate (150), and the solution discharged downward can be sprayed onto the membrane so that the antigen is coated on the membrane.

[0044] As illustrated in FIG. 4, the suction unit (200) may be provided with a passage (220) that provides a path for air to be transported. The passage (220) may be provided in multiple numbers. FIG. 4 illustrates a case where three passages (220) are provided.

[0045] Additionally, the suction unit (200) is connected to the flow path (220), and a negative pressure forming section (not shown) capable of forming negative pressure inside may be formed. According to the present invention, when air present inside the negative pressure forming section is removed, the inside of the negative pressure forming section becomes a negative pressure state, which is lower than normal pressure. Therefore, a pressure difference exists between the external pressure (i.e., normal pressure) and the pressure inside the negative pressure forming section (i.e., negative pressure). At this time, when the flow path (220) of the suction unit (200) is opened, external air is supplied into the negative pressure forming section due to the difference between the pressure inside the negative pressure forming section and the external pressure, and at the same time, the antigen contained inside the upper plate (100) is sprayed onto the membrane and coated.

[0046] Accordingly, the method for manufacturing an allergy diagnostic chip according to the present invention may include a step of forming a coating portion, wherein a solution containing antigens within an upper plate (100) is moved downward by forming a negative pressure, which is lower than normal pressure, inside a suction unit (200), thereby forming a plurality of line-shaped coating portions on which antigens are adsorbed on a membrane.

[0047] Meanwhile, negative pressure within the negative pressure forming section can be formed by the discharge of air from within the section to the outside. Subsequently, during the coating section formation stage, air is introduced into the negative pressure forming section, and accordingly, the pressure within the negative pressure forming section gradually increases. Eventually, when the pressure within the negative pressure forming section becomes equal to atmospheric pressure, the coating section formation stage can be terminated.

[0048] In the coating formation step of the method for manufacturing an allergy diagnostic chip according to the present invention, negative pressure may be formed for 7 seconds to 120 seconds. If negative pressure is formed for less than 7 seconds in the coating formation step, a problem may occur where the solution containing the antigen does not properly coat the membrane in a line shape and spreads during the process of coating the antigen on the membrane. Conversely, if negative pressure is formed for more than 120 seconds in the coating formation step, the process of coating the antigen on the membrane consumes an excessive amount of time, which may reduce productivity. More preferably, negative pressure may be formed for 7 seconds to 90 seconds in the coating formation step.

[0049] Additionally, in the coating formation step, the difference between the negative pressure and the atmospheric pressure may be 1.8 bar to 2.2 bar. If the difference between the negative pressure and the atmospheric pressure in the coating formation step is less than 1.8 bar, the speed at which the solution within the upper plate (100) is sprayed during the antigen coating process of the membrane may not be sufficient, and the quality of the antigen coating may be degraded. On the other hand, if the difference between the negative pressure and the atmospheric pressure in the coating formation step exceeds 2.2 bar, the speed at which the solution is sprayed during the antigen coating process of the membrane and the speed of the air entering the negative pressure forming part may become excessively large, and the membrane may be damaged.

[0050] Referring again to FIGS. 1 and 2, the manufacturing device (10) may further include a pressurizing unit (300) that presses the upper plate (100) downward. The pressurizing unit (300) according to the present invention may be configured to allow the coating of antigens on the membrane provided on the upper surface of the lower plate (150) to be smoothly performed by lowering the upper plate (100) to press against and adhere to the lower plate (150) during the process of coating antigens on the membrane.

[0051] As illustrated in FIG. 2, the pressure unit (300) may include a side pressure unit (310) that presses both sides of the upper part of the upper plate (100) downward and a central pressure unit (320) that presses the central part of the upper plate (100) downward. The side pressure unit (310) and the central pressure unit (320) may be driven independently of each other.

[0052] Accordingly, the method for manufacturing an allergy diagnostic chip according to the present invention may further include a pressurizing step of additionally pressurizing a portion of the upper region of the coating unit so that the membrane can be adhered downward. The pressurizing step according to the present invention may be performed between the aforementioned membrane provisioning step and the coating portion formation step.

[0053] Referring further to FIGS. 1 and FIGS. 2, a step-shaped step (310a) may be formed on the inner side of the side pressure member (310). At this time, the step (310a) may have a shape corresponding to both sides of the upper plate (100). FIG. 2 illustrates a case where both sides of the upper plate (100) are formed vertically, and the step (310a) facing both sides of the upper plate (100) is also formed vertically. When the step (310a) has a shape corresponding to both sides of the upper plate (100), the side pressure member (310) not only performs the role of pressing the upper plate (100) downward, but also performs the role of gripping the upper plate (100) so that the upper plate (100) does not shake left or right.

[0054] FIG. 5 is a perspective view illustrating the structure of an example of an upper plate in a manufacturing device that can be used in the present invention, FIG. 6 is a plan view illustrating the structure of an example of an upper plate in a manufacturing device that can be used in the present invention, and FIG. 7 is a cross-sectional view illustrating the structure of an upper plate cut along line AA of FIG. 6.

[0055] According to one example as illustrated in FIGS. 5 to 7, the upper plate (100) may include an upper surface (112) in which an injection port (112a) for injecting a solution containing an antigen is formed, and a lower surface (114) provided at the bottom of the upper surface (112) through which the solution containing the antigen is discharged toward a lower plate (150, see FIG. 2). According to one example of the upper plate according to the present invention as illustrated in FIGS. 5 to 7, the injection port (112a) of the upper plate (100) may be a plurality of supply holes having a dot shape. Hereinafter, the reference numeral 112a will be referred to as a 'supply hole' in this specification.

[0056] Meanwhile, in one example of the upper plate (100) of the present invention, a plurality of slits (114a) having a line shape may be formed in the lower portion (114). According to the present invention, a solution containing antigens can be discharged in a line shape through the plurality of slits (114a) formed in the lower portion (114) of the upper plate (100), thereby allowing the antigens to be coated on the membrane in a line shape. That is, in this specification, the slit (114a) does not refer to a physical configuration, but rather to a space through which the solution containing antigens is discharged.

[0057] The width of the plurality of slits (114a) formed on the lower portion (114) of the upper plate (100) may be 0.2 mm to 0.5 mm. According to the present invention, since the width of the plurality of slits (114a) formed on the lower portion (114) of the upper plate (100) is 0.2 mm to 0.5 mm, when coating a solution containing an antigen onto a membrane in a line shape, the thickness of the line coated on the membrane can also be 0.2 mm to 0.5 mm. Therefore, by reducing the thickness of the line coated on the membrane compared to the prior art, high integration of the diagnostic chip can be achieved. More preferably, the width of the plurality of slits (114a) formed on the lower portion (114) of the upper plate (100) may be 0.3 mm to 0.5 mm.

[0058] Meanwhile, as described above, in the coating portion formation step, a solution containing antigen is discharged through a plurality of slits (114a) formed on the lower portion (114) of the upper plate (100), so the thickness of the coating portion can correspond to the width of the slits (114a). That is, the thickness of the plurality of coating portions formed in the coating portion formation step may be 0.2 mm to 0.5 mm. More preferably, the thickness of the plurality of coating portions may be 0.3 mm to 0.5 mm.

[0059] Referring further to FIGS. 5 to 7, the upper plate (100) may include a connecting portion (116) formed on the upper surface portion (112) and the lower surface portion (114) and connecting the upper surface portion (112) and the lower surface portion (114). The connecting portion (116) of the upper plate (100) may be understood as forming the body of the upper plate (100).

[0060] At this time, as shown in FIG. 7, a plurality of thin plates (116a) may be provided spaced apart from each other inside the connecting portion of the upper plate (100). A plurality of internal spaces that are sealed from each other may be formed between the plurality of thin plates (116a), and a solution containing antigen may be contained in the internal spaces between the plurality of thin plates (116a).

[0061] Meanwhile, a plurality of internal spaces formed by a plurality of thin plates (116a) provided on the upper plate (100) may each communicate with one of a plurality of slits (114a) formed on the lower surface of the upper plate (100). Additionally, each of a plurality of supply holes (112a) formed on the upper surface (112) of the upper plate (100) may communicate with one of a plurality of internal spaces formed on the connecting portion (116) of the upper plate (100).

[0062] Accordingly, a solution containing antigen supplied through one of the supply holes (112a) of the upper plate (100) can be introduced into a plurality of internal spaces formed by a plurality of thin plates (116a) and connected to the supply hole (112a), and then discharged downward through a slit (114a) connected to the plurality of internal spaces.

[0063] The thickness of the plurality of thin plates (116a) formed in the connecting portion (116) of the upper plate (100) may be 0.5 mm to 0.9 mm.

[0064] Meanwhile, as illustrated in FIG. 7, the slits (114a) formed on the lower portion (114) of the upper plate (100) can be connected to the ends of the plurality of internal spaces formed by the plurality of thin plates (116a) provided in the connecting portion (116). Additionally, the spacing between the slits (114a) formed on the lower portion (114) of the upper plate (100) may be equal to the thickness of the plurality of thin plates (116a) provided in the connecting portion (116). Accordingly, the thickness of the thin plates (116a) may be equal to the spacing between the coating portions formed in the coating portion formation step. That is, in the coating portion formation step of the method for manufacturing an allergy diagnostic chip according to the present invention, the spacing between the plurality of coating portions may be 0.5 mm to 0.9 mm. If the spacing between multiple coating sections is less than 0.5 mm, the thin plate (116a) may be damaged by the rapid fluid flow caused by the operation of the suction unit (200) during the process of coating antigens on the membrane. On the other hand, if the spacing between multiple coating sections exceeds 0.9 mm, the width between the coating sections coated on the membrane becomes excessively large, making it impossible to achieve high integration of the diagnostic chip.

[0065] The supply hole (112a) on the upper surface formed on the upper plate (100), the internal space formed by a plurality of thin plates (116a), and the slit (114a) on the lower surface can be connected to each other in a one-to-one manner. Accordingly, the number of supply holes (112a) formed on the upper surface (112) of the upper plate (100), the number of slits (114a) formed on the lower surface (114) of the upper plate (100), and the number of internal spaces formed on the connecting portion (116) of the upper plate (100) can be the same as each other. Although FIG. 7 is illustrated as if some of the multiple internal spaces formed in the connecting portion (116) of the upper plate (100) do not communicate with the supply hole (112a) of the upper surface portion (112), as illustrated in FIG. 6, the supply hole (112a) can be formed over the entire area of ​​the upper surface portion (112) of the upper plate, so the content illustrated in FIG. 7 should not be understood as not conforming to the content of this paragraph.

[0066] However, unlike this, the supply hole (112a) on the upper surface formed on the upper plate (100) may correspond to an internal space formed by a plurality of thin plates (116a) in a ratio of n (where n is an integer greater than or equal to 2) to 1. For example, the supply hole (112a) on the upper surface formed on the upper plate (100) may correspond to an internal space formed by a plurality of thin plates (116a) in a ratio of 2 to 1.

[0067] FIG. 8 is a perspective view illustrating the structure of another example of an upper plate among a manufacturing device that can be used in the present invention, FIG. 9 is a plan view illustrating the structure of another example of an upper plate among a manufacturing device that can be used in the present invention, and FIG. 10 is a cross-sectional view illustrating the structure of an upper plate cut along the BB line of FIG. 9. In the following description of another example of an upper plate, parts that overlap with the description of one example of an upper plate will be omitted, and the description will focus on the content that differs from the one example of an upper plate.

[0068] According to another example as illustrated in FIGS. 8 and 9, a plurality of slits (112b) having a line shape may be formed as injection ports on the upper surface (112) of the upper plate (100) so that a solution containing antigen can be supplied to the upper plate (112), and each of the plurality of slits (112b) formed on the upper surface (112) of the upper plate (100) may be in communication with one of the plurality of internal spaces formed in the connecting portion (116) of the upper plate (100).

[0069] Accordingly, a solution containing antigen supplied through one of the slits (112b) of the upper surface portion (112) of the upper plate (100) can be introduced into a plurality of internal spaces formed by a plurality of thin plates (116a) and connected to the slit (112b), and then discharged downward through a slit (114a) of the lower portion (114) connected to the plurality of internal spaces.

[0070] Additionally, the slit (112b) on the upper surface formed on the upper plate (100), the internal space formed by a plurality of thin plates (116a), and the slit (114a) on the lower surface can be connected to each other in a one-to-one manner. Accordingly, the number of multiple slits (112b) formed on the upper surface (112) of the upper plate (100), the number of multiple slits (114a) formed on the lower surface (114) of the upper plate (100), and the number of multiple internal spaces formed on the connecting portion (116) of the upper plate (100) can be the same. Although FIG. 10 shows that some of the multiple internal spaces formed in the connecting portion (116) of the upper plate (100) do not communicate with the slit (112b) of the upper surface portion (112), as shown in FIG. 9, the slit (112b) is formed over the entire area of ​​the upper surface portion (112) of the upper plate, so the content shown in FIG. 9 should not be understood as not being consistent with the content of the above paragraph.

[0071] Meanwhile, the upper plate (100) may have a shape formed by combining the aforementioned supply hole (112a) and slit (112b). That is, the upper plate (100) according to the present invention may have a structure in which both the supply hole (112a) and the slit (112b) are formed.

[0072] FIG. 11 is a perspective view illustrating the structure of a lower plate among a manufacturing apparatus that can be used in the present invention, and FIG. 12 is a side cross-sectional view illustrating the structure of a lower plate cut along the CC line of FIG. 11.

[0073] As illustrated in FIGS. 11 and 12, the lower plate (150) may include an upper surface (162) in which a plurality of slits (162a) are formed and a lower surface (164) in which a plurality of slits (164a) are formed. Similar to the case of the upper plate, the slits (162a, 164a) formed on the lower plate (150) also represent physical spaces.

[0074] The slits (162a, 164a) formed on the upper surface (162) and lower surface (164) of the lower plate (150), respectively, can provide a path for external air to flow into the suction unit during the process of coating the membrane with a solution containing an antigen. That is, as described above, when negative pressure is formed inside the suction unit (200) during the membrane coating process, external air can be supplied to the suction unit after sequentially passing through the slit (162a) formed on the upper surface (162) and the slit (164a) formed on the lower surface (164) of the lower plate (150). Meanwhile, similar to the case of the upper plate, a connecting portion connecting the upper surface (162) and the lower surface (164) can be formed on the lower plate (150), and a plurality of thin plates spaced apart from each other can be formed inside the connecting portion. Each internal space between multiple thin plates can be connected to one of the slits (162a) of the upper portion (162) and one of the slits (164a) of the lower portion (164).

[0075] Meanwhile, in the upper plate according to the present invention, the ratio of the width of the slit to the thickness of the thin plate may be 0.40 to 0.55.

[0076] FIG. 13 is a plan view illustrating the structure of an allergy diagnostic chip manufactured according to the present invention.

[0077] As illustrated in FIG. 13, the allergy diagnostic chip (400) according to the present invention may be a configuration manufactured by the allergy diagnostic chip manufacturing method according to the present invention described above.

[0078] The allergy diagnostic chip (400) may include a sheet-shaped membrane (400a) and a coating portion (410) which is an area coated with an antigen on the membrane (400a). The coating portion (410) may be formed in multiple numbers and may have a band shape with a constant thickness.

[0079] The allergy diagnostic chip (400) may have a rectangular shape with a constant width (W) and length (L), and a plurality of coating portions (410) having a constant thickness (t) may be provided spaced apart from each other by a constant distance (d) along the width (W) direction of the allergy diagnostic chip (400).

[0080] The allergy diagnostic chip (400) according to the present invention can form a large number of coating portions relative to the same area of ​​the chip compared to the prior art, thereby enabling high integration of the allergy diagnostic chip (400). To this end, the allergy diagnostic chip (400) according to the present invention may have a constant ratio between the spacing (d) between the coating portions (410) and the thickness (t) of the coating portions (410).

[0081] That is, in the allergy diagnosis chip (400) according to the present invention, the ratio of the thickness (t) of the coating portion (410) to the spacing (d) between the coating portions (410) (i.e., t / d) may be 0.40 to 0.55.

[0082] In addition, according to the present invention, the thickness (t) of the coating portion (410) may be 0.2 mm to 0.5 mm. In addition, the spacing (d) between the coating portions (410) may be 0.5 mm to 0.9 mm.

[0083] Meanwhile, damage to the membrane may occur even though the difference between the atmospheric pressure and the negative pressure is controlled within a certain numerical range during the coating formation step of the present invention. For example, during the coating formation step, external air is introduced into the suction device through the lower plate due to the pressure difference between the negative pressure and the atmospheric pressure. When the membrane provided on the upper surface of the lower plate (150) comes into direct contact with the upper surface (162) of the lower plate (150), problems such as being sucked into the slit (162a) of the upper surface (162) may occur.

[0084] Accordingly, to prevent the above problem, the membrane provision step of the method for manufacturing an allergy diagnostic chip according to the present invention may further include providing a protective plate between the suction unit (200) and the membrane to prevent damage to the membrane caused by the difference between negative pressure and atmospheric pressure. The protective plate may include a cotton material. If the protective plate includes a cotton material or is made of a cotton material, damage to the membrane due to the material of the protective plate itself can be prevented during the coating formation step.

[0085]

[0086] Example 1

[0087] After fixing the lower plate to the upper part of the space of the suction unit through fastening, a membrane was provided on the upper surface of the lower plate. Additionally, both sides of the upper plate were gripped by lateral pressure parts, and the lateral and central pressure parts were moved downward to bring the upper plate into close contact with the lower plate and the membrane. Furthermore, air inside the negative pressure generating part of the suction unit was discharged to create a negative pressure state 2 bar lower than atmospheric pressure.

[0088] Subsequently, by opening the air passage of the suction unit, external air flowed into the negative pressure generating section, and a solution containing antigens was coated onto the membrane to manufacture a diagnostic chip. The air passage of the suction unit was opened for 7 seconds. As air flowed into the negative pressure generating section, the pressure inside the negative pressure generating section gradually increased, and after the coating was completed, the pressure inside the negative pressure generating section reached atmospheric pressure.

[0089]

[0090] Example 2

[0091] Except for the fact that the flow path of the suction unit was open for 30 seconds, the membrane was coated with a solution containing an antigen in the same manner as in Example 1.

[0092]

[0093] Example 3

[0094] Except for the fact that the flow path of the suction unit was open for 60 seconds, the membrane was coated with a solution containing an antigen in the same manner as in Example 1.

[0095]

[0096] Example 4

[0097] Except for the fact that the flow path of the suction unit was open for 120 seconds, the membrane was coated with a solution containing the antigen in the same manner as in Example 1.

[0098]

[0099] Comparative example

[0100] Except for the fact that the flow path of the suction unit was open for 5 seconds, the membrane was coated with a solution containing an antigen in the same manner as in Example 1.

[0101]

[0102] Experimental Example 1

[0103] The antigen coating state of the diagnostic chips manufactured according to the examples and comparative examples was observed. The results of the observation are shown in FIG. 14. FIG. 14 (a) shows the coating state of the diagnostic chip according to the comparative example, and FIG. 14 (b) to (e) show the coating state of the diagnostic chips according to Examples 1 to 4, respectively.

[0104] As shown in Fig. 14(a), in the comparative example, it was observed that some of the solution containing the antigen had spread around the line coated on the diagnostic chip. In other words, in the comparative example, it was confirmed that the coating on the membrane was not properly formed because the time for the negative pressure to be formed was not sufficiently secured.

[0105] As shown in Figures 14 (b) to (e), in the case of Examples 1 to 4, it was confirmed that the coating was successfully applied only to the lines coated on the diagnostic chip.

[0106]

[0107] Experimental Example 2

[0108] The thickness (t) of the coating layer coated with the antigen-containing solution and the spacing (d) between the coating layers were measured in the diagnostic chips prepared according to Examples 1 to 4 and Comparative Example.

[0109] In the diagnostic chips manufactured according to Examples 1 to 4, the thickness (t) of the coating layer was measured to be 0.4 mm, and the gap (d) between the coating layers was measured to be 0.75 mm. Accordingly, the ratio (t / d) of the thickness (t) of the coating layer to the gap (d) between the coating layers was measured to be 0.533.

[0110] On the other hand, in the comparative example, the solution was not coated uniformly, and some of the solution spread around the coated lines, so the thickness of the coating layer and the spacing between coating layers could not be measured.

[0111]

[0112] Although the present invention has been described above with reference to limited embodiments and drawings, the present invention is not limited thereto, and it is obvious that various implementations are possible within the scope of the technical spirit of the present invention and the equivalent scope of the claims described below by those skilled in the art to which the present invention belongs.

[0113]

[0114]