Peptides labeled with tracer molecules for the detection of the P16 ink4a protein and the protocol for using such peptides.

Abstract

DC60 (12 / 07 / 55) This invention describes the fabrication of a p16INK4a protein detection molecule and a method for using that molecule. As mentioned, in cell or tissue samples, the peptide was modified and labeled to specifically bind to the p16INK4a protein. Using chemical molecules, biochemical molecules, or trace particles on a peptide chain where the peptide has a specific amino acid sequence. Any one of the three amino acid sequences (peptide sequence 1, peptide sequence 2, and peptide...) The third amino acid sequence) as described in the complete invention disclosure by the important properties of The peptide that monitors the protein p16INK4a, all three amino acid sequences are small, linear peptides consisting of 7 sequences. Amino acids enable the peptide tracer according to this invention to have the ability to penetrate cells or tissues. The sample binds well to the p16INK4a protein found in cells, thus providing a clear detection signal. It is 8-14 times more effective than using antibodies, and this is also a result of the peptide according to this invention. High specificity and stability are required, while currently available antibodies against p16INK4a protein are... The molecule is approximately 150 times larger than the peptide detected by this invention, and the antibody must... It relies on the influence of a suitable structural form to facilitate the reaction at a specific binding site, whereas peptides do not. These properties are necessary for the preservation of peptides, their detection, and their diverse applications. It is easy to use and suitable for application in daily pathology examinations and the synthesis of diagnostic peptides. High-purity traces can be produced in large quantities using peptide synthesizers based on specific techniques. Synthesize basic peptides that are currently available or rely on mimicking natural processes to produce ready-to-use peptides. Particles are labeled by assigning peptides for expression on the surface of bacteriophage particles, thereby inducing peptide production. Monitoring is a low-cost and highly effective solution for industrial-scale production processes. This invention describes the fabrication of a p16INK4a protein detection molecule and a method for using that molecule. As mentioned in cell or tissue samples, this was achieved by modifying and labeling the p16INK4a protein with a specific binding peptide. Using chemical substances, biochemical molecules, or trace particles on a peptide chain, where the peptide has a specific sequence of amino acids. Any one of the three amino acid sequences (peptide sequence 1, peptide sequence 2, and peptide...) The third amino acid sequence) as described in the complete invention disclosure, by the important properties of The peptides that monitor the p16INK4a protein, all three amino acid sequences, are small, linear peptides consisting of 7 sequences. Amino acids enable the peptide tracer described in this invention to have the ability to penetrate cells or tissues. The sample binds well to the p16INK4a protein present in cells, thus providing a clear detection signal. It is 8-14 times more effective than using antibodies. Furthermore, this is due to the properties of this peptide. High specificity and stability are required, while currently available antibodies specifically binding to p16INK4a protein are... The molecule is approximately 150 times larger than the peptide detected according to this invention, and the antibody must... It relies on the influence of a suitable structural form to facilitate the reaction at a specific binding site, whereas peptides do not. These properties are necessary for the preservation of peptide detectors and their diverse applications. It is easy to use and suitable for application in routine pathology examinations and the synthesis of diagnostic peptides. High-purity traces can be produced in large quantities using peptide synthesizers based on specific techniques. Synthesize basic peptides that are currently available or rely on mimicking natural processes to produce ready-to-use peptides. Particles are labeled by inducing peptide expression on the surface of bacteriophage particles, thereby enabling peptide production. Monitoring is a low-cost and highly potential solution in industrial-scale production processes;

Claims

1. A peptide labeled with a tracer molecule specific to the detection of the p16INK4a protein, where the peptide has a selectable amino acid sequence from either amino acid sequence 1: SHSLLHH(ser-his-ser-leu-leu-his-his), amino acid sequence 2: SLHQPHL(ser-leu-his-gln-pro-his-leu), or amino acid sequence 3: YAWDTYR(tyr-ala-trp-asp-thr-tyr-arg);