The components of a 3D-printed artificial bone network combined with hydroxyapatite nanoparticles for microRNA delivery, and the manufacturing process.
Patent Information
- Application Number
- TH2201002388
- Authority / Receiving Office
- TH · TH
- Patent Type
- Patents
- Current Assignee / Owner
- Filing Date
- 2022-04-22
- Publication Date
- 2026-09-05
- Estimated Expiration
- 2042-04-21
Claims
OCR09WP13 / 07 / 25691. Components of a 3D-printed artificial bone network combined with hydroxyapatite nanoparticles for microRNA delivery: a. Hydroxyapatite and tricalcium phosphate using calcium and phosphate precursors in a calcium-to-phosphate ratio of 1.35-1.5:
1. b. MicroRNA particles of 180-200 nanograms per 30-70 micrograms of hydroxyapatite particles. c. Hydroxyapatite nanoparticles of 30-70 micrograms per 13-15 cubic millimeters of artificial bone network volume.
2. Manufacturing process of the artificial bone network... The artificial bone network, created using 3D printing and incorporating hydroxyapatite nanoparticles for microRNA delivery, involves the following steps: a. Designing the artificial bone network structure using printer software. This creates a mesh-like structure with voids that allow cells to grow and infiltrate the network, forming bone and blood vessels. b. Loading the tricalcium phosphate and hydroxyapatite cream material into the printer's nozzle and printing the artificial bone network. The resulting bone network will conform to the designed structure. c. ...Alternatively, in step b, immerse the artificial bone network designed in water and add 30-70 µg of hydroxyapatite particles infused with 180-200 nanograms of microRNA per 13-15 cubic millimeters of artificial bone. Leave it in the water for 48 hours until the bone network solidifies. This will result in a 3D-printed artificial bone network combined with nanoscale hydroxyapatite particles for microRNA delivery. Or, in step d, immerse the artificial bone network designed in step b in water for 48 hours until it solidifies, then drop 30-70 µg of hydroxyapatite particles infused with 180-200 nanograms of microRNA per 13-15 cubic millimeters of artificial bone onto the bone network. This will result in a 3D-printed artificial bone network combined with nanoscale hydroxyapatite particles for microRNA delivery.