The process of producing sodium carbonate (Na2CO3) through microbial processes using the bacterium Sporosarcina pasteurii.

Abstract

Invention details;

Claims

OCR06WT1. The microbial process of producing sodium carbonate (Na2CO3) using the bacterium Sporosarcinapasteurii involves the following steps: a. Preparation of the culture medium: Begin by preparing a 0.13 M Trisbuffer solution (130 ml). Then, add 2% by weight of yeast extract and 1% by weight of ammonium sulfate ((NH4)2SO4) to the container. Next, add deionized water to reach 100% by weight of the total volume. Cover the container with foil. Sterilize the culture medium by steam autoclaving at 121°C / 200 psi for 15 minutes. Then, allow it to cool to room temperature. b. Culture the bacteria: Add 0.2% of the total volume of Sporosarcinapasteurii bacteria to the prepared culture medium from step a.C. Incubate the bacteria under controlled conditions at 30°C and a shaking speed of 150 rpm for 48 hours. Monitor bacterial growth by measuring cell density until the bacteria reach a density of 25 x 10⁹ cells per milliliter (ml). D. Centrifuge the bacteria obtained from step b at 4,500 rpm for 10 minutes to separate them from the original culture medium. Discard the old culture medium and add a 4 molar urea (CO(NH2)2) solution, 10% of the old culture medium volume, to the bacteria. Mix thoroughly to distribute the bacteria in the solution. E. Incubate the bacteria in the urea (CO(NH2)2) solution obtained from step c.At 30°C and shaken at a speed of 150 rpm for 8 hours, the bacteria break down urea, producing ammonium carbonate ([NH4]2CO3) and ammonia (NH3). The mixture is incubated until the ammonia concentration reaches 8 molar, resulting in an ammonium carbonate solution concentration of 4 molar. The resulting ammonium carbonate solution is then separated, filtered, and stored in a sealed container. The ammonium carbonate solution contains carbonate ions (CO32-) in solution form. It can be used in the crystallization process of sodium carbonate (Na2CO3) in the next step. D. Dilute the ammonium carbonate ([NH4]2CO3) solution obtained from step E to a concentration of 1 molar. Then, mix it with sodium hydroxide (NaOH) at a concentration of 5-8% by weight / volume of the solution. Leave it to react for 24 hours to obtain a sodium carbonate (Na2CO3) solution. F. Evaporate the sodium carbonate (Na2CO3) solution obtained from step E at 100°C to crystallize sodium carbonate (Na2CO3) and separate ammonia (NH3) from the system.

1. The solution from point f is filtered to separate the sodium carbonate (Na2CO3) crystals. The crystals are then dried at 120°C for 24 hours, then ground and sieved, and stored in packaging to obtain ready-to-use sodium carbonate.

2. The process of producing sodium carbonate (Na2CO3) through microbial processes using the bacterium Sporosarcinapasteurii, as per patent 1, which includes an additional step to accelerate the crystallization of sodium carbonate (Na2CO3). The sodium carbonate (Na2CO3) solution from point f, evaporated to 50% volume, is left to cool at room temperature for 1 hour. Then, 95% ethyl alcohol is added to the solution at a volume ratio of alcohol to sodium carbonate solution (L / L) of 0.5-1.5 to 1. The solution is then incubated at 3-25°C to accelerate the crystallization of sodium carbonate (Na2CO3). Crystallization can be achieved within 2 hours.

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