This study investigated the process of producing glycolipid-based biosurfactants from the yeast genus Starmerella sp. by adjusting the carbon-to-nitrogen ratio, using sodium chloride as an activator, and obtaining semi-purified, bioactive compounds.

Claims

OCR07AP1. The process of producing glycolipid biosurfactants from the yeast genus Starmerella sp. by adjusting the carbon-to-nitrogen ratio, using sodium chloride as an activator, and obtaining semi-purified bioactive compounds consists of the following steps: a. The process of producing glycolipid biosurfactants from Starmerella sp. yeast using glucose and palm oil as carbon sources, adjusting the carbon-to-nitrogen ratio under sodium chloride activation, and using batch glycolipid fermentation. The process involves the following steps: - Cultivate Starmerella sp. yeast in YPD liquid medium containing 2% glucose by weight / volume (50 ml or 400 ml) at a temperature of 28-32°C with a shaking rate of 150-250 rpm for 18-24 hours. - Transfer 50 ml of starter culture to a culture medium containing 10% glucose, 10% palm oil, 0.45-3.6% yeast extract, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate heptahydrate, 0.01% calcium chloride, and 0.01-3.0% sodium chloride. Adjust the initial pH to 4.5 and the initial OD600 value to 1.

0. Shake at 150-250 rpm, incubate at 28-32°C for 3-7 days, and measure the amount of glycolipids produced. - Application of the same conditions in a 5-liter bioreactor using 400 ml of starter culture placed in 4 liters of culture medium with the components as in the previous step, and fermented at 30°C, stirring speed 150-450 rpm, aeration rate 1.0 vvm for 3-7 days. b. The process of extracting glycolipid biosurfactant from the yeast Starmerella sp. obtained from the fermentation process consists of the following steps: - When finished... At the end of the fermentation process, cells are separated from the fermentation broth using a centrifuge at 5,000 rpm for 5-10 minutes. The fermentation broth is then extracted with ethyl acetate at a ratio of 1:1 to 1:2 (v / v) for two cycles, shaking for 5-15 minutes per cycle. The ethyl acetate layer is then combined, and the solvent is evaporated using a rotary distillation at 40-45 degrees Celsius under a pressure of 100-300 mbar to obtain a crude glycolipid extract. This is a process for separating semi-purified bioactive compounds from the biosurfactant glycolipid from the yeast Starmerella sp.The procedure involves the following steps: - Separation of semi-purified compounds from crude extract using column chromatography on silica gel with chloroform and methanol as eluents. - Sample preparation by dissolving the crude extract in 1% methanol in chloroform (v / v) at a 1:1 ratio, totaling 1 ml, and packing it into a chloroform-saturated column. - Eluting the column with 10 ml of the 1% methanol in chloroform solution, and collecting 20 ml of fraction A. - Collecting 20 ml of fraction B following the eluence in the next step. - Evaporation of the solvent using a rotary distillation apparatus at 40-44 °C under a pressure of 100-300 mbar.