A flow-based biologic analyzer combined with electrochemical techniques for the differential diagnosis of three types of respiratory infectious diseases: coronavirus infection, influenza, and respiratory syncytial virus (RSV).

Abstract

This invention concerns a flow-channel bioanalytical device combined with a chemotherapy technique. Electrochemistry for diagnostic purposes classifies three types of respiratory tract infections: coronavirus infections, etc. This invented device can treat coronavirus, influenza, and RSV infections simultaneously. A transparent sheet with a polymer coating to make the transparent sheet hydrophilic (11) that is screen printed. Counter electrode (CE) positions (5), (8), (10) and reference electrode RE) positions (7), (6), (9) and there is a hole at position 1 for dropping the mixed solution. Potassium ferrocyanide and potassium ferricyanide for measurement are placed in this layer, which is positioned on top. This fabricated measuring device is followed by a layer of flow channels (13) made of double-sided adhesive tape with Drill a flow channel at position (12) to provide a flow path for the potassium ferro solution. Cyanide and potassium ferricyanide can move from position (1) to the detection area at position (20), (21), and (22) are obtained. The next sequence is the flow channel layer (15), which is made of transparent sheets with a polymer coating. Attach it to the double-sided tape and make a hole at position (14) to allow the potassium solution to mix. Ferrocyanide and potassium ferricyanide are confined to the electrode junction area. The work involves only the auxiliary electrodes and reference electrodes, and the bottom layer is a paper base layer with wax printing. (wax) (19) To define the boundaries of the hydrophilic and hydrophobic parts by the front of the paper base device. Consists of screen-printed electrodes for the detection of influenza virus (H1N1) (16), viruses RSV (17) and coronavirus (SARS CoV-2) (18) and the posterior part at positions (20), (21), and (22) The position corresponding to the working electrode will be used to set up a specific nucleic acid biosensor. The influenza virus (H1N1), the respiratory virus (RSV), and the coronavirus are shown in layers, respectively. The improvised devices can be assembled together using double-sided tape.

Claims

1. Flow-based bioassay device combined with electrochemical techniques for diagnosis. Infectious respiratory diseases are classified into three types: coronavirus infection, influenza, and other diseases. RSV infection can occur in a single infection and involves four parts: Part 1 is a transparent sheet layer that is coated with a polymer to make the transparent sheet hydrophilic (11). This transparent sheet will be screen printed with counter electrodes (CE) at positions (5), (8), (10) and Reference electrode (RE) position (7), (6), (9) is the top part of the measuring device. And there is also drilling of a hole (1) with a diameter of 3 - 4 millimeters with a laser machine for use in dropping. A mixed solution of potassium ferrocyanide and potassium ferricyanide ([Fe(CN)6]3- / 4-) in Electrochemical signal tracking with amperometry technique and positions (2), (3), (4) diameter. A center of 0.8 - 1 millimeter is measured using a laser to vent air within the flow channel during the measurement. To prevent residue or air bubbles from forming within the flow channel, as this residue of air bubbles can cause problems. It can interfere with the measurement signal. Part 2. Flow channel layer (13), which is made of double-sided adhesive tape with a thickness of 100-200 micrometers, with Drilling flow channels at specific locations (12 sizes, 3-4 mm diameter, using a laser) A mixed solution of potassium ferrocyanide and potassium ferricyanide can flow through from... Part 1 leads to Parts 3 and 4. Part 3. Flow channel layer (15), which is made of a transparent sheet with a polymer coating attached to double-sided adhesive tape. Front and drill a hole at position (14) with a diameter of 3 - 4 millimeters to create a flow channel. From part 2, transfer the mixed solution of potassium ferrocyanide and potassium ferricyanide to... Part 4 also has the important function of containing this mixed solution to cover the measurement area, which is the vicinity... A junction consisting of a working electrode (WE), an auxiliary electrode (CE), and a reference electrode. (RE) Part 4: A base paper layer with wax printing to define the boundaries of the hydrophilic zone. Hydrophobic part (19) The front of the paper base device consists of a screen-printed electrode of size Diameter 3 - 4 mm for the detection of influenza virus (H1N1) (16), RSV virus (RSV) (17) and coronavirus (SARS-CoV-2) (18) and the posterior part at positions (20), (21), and (22) at The corresponding electrode positions are used to anchor the nucleic acid biomarker specific to the virus. Influenza (H1N1), Respiratory Syncytial Virus (RSV), and Coronavirus, respectively. This section is responsible for... Detect the genetic material of the virus before performing measurements using amperometry.

2. A flow-based bioassay device combined with electrochemical techniques, as per claim 1. This involves the immobilization of nucleic acid biomarkers specific to the influenza virus (H1N1) and the RSV virus. (RSV and coronavirus at a concentration of 0.5 - 2.0 micromolar for this detection device to be able to detect them.) And it can diagnose all three types of viral infections at once using only one measuring device.

3. Flow-based bioassay device combined with electrochemical techniques, as per claim 2. This uses a solution mixed with potassium ferrocyanide and potassium ferricyanide ([Fe(CN)6]3- / 4-). Concentrations of 5-10 millimolar were used to track electrochemical signals using amperometry.

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