Formulations and manufacturing processes for powdered probiotic mixes related to the human liver.

Abstract

OCR 10KL (10 / 10 / 2568) Formulations and manufacturing processes for powdered probiotic mixes related to the human liver. It consists of: 1. Lactobacillus reuteri (TISTR2736) 2. Lactobacillus 3. Trehalose sugar powder (Lactobacillus zeae TISTR2529). The manufacturing process begins with... Each bacterial strain was isolated and cultured using specific types of culture media and cultivation conditions. The concentration was adjusted to achieve 10⁸ - 10⁻¹⁰ colonies per gram. Then, the bacterial cells were washed and separated. The culture was prepared using a sodium chloride solution, centrifuged, and the resulting solid culture was stored in trehalose sugar. It is in powder form and then mixed, starting with adding the Lactobacillus reuteri (TISTR2736) powder. Next, add Lactobacillus zeae (TISTR2529) powder, followed by the probiotic powder. The mixed ingredients are immediately placed in vacuum-sealed foil bags.

Claims

OCR 10KL (10 / 10 / 2568) 1. The formula for producing a powdered probiotic mix related to the human liver consists of: The percentage of Lactobacillus reuteri bacteria was 36.42 - 47.87% by weight per volume. (Lactobacillus reuteri TISTR2736) The percentage of Lactobacillus c.e. bacteria was 34.52 - 46.87% by weight per volume. (Lactobacillus zeae TISTR2529) Powdered trehalose sugar, 15.53% - 18.81% by weight / volume.

2. The process for manufacturing a powdered probiotic mixture involving the human liver, as per claim 1, which includes: The steps are as follows: Bacterial culture a. Isolated and cultured the bacterial strain Lactobacillus reuteri (TISTR2736) and... Lactobacillus zeae (TISTR2529) was studied using specific culture media and conditions. Specific culture methods suitable for the growth of each bacterial strain. B. Culture each bacterial strain until it reaches the initial stationary stage and obtains the desired concentration of... The bacterial count is in the range of 10⁸ to 10⁻¹⁰ colonies per gram. Washing and separating bacterial cells. C. Wash and isolate each strain of bacteria using a sterile sodium chloride solution. Concentration of 0.50-1.50% by weight per volume, using centrifugal force at a certain speed. Operating at 5,500-9,500 revolutions per minute takes 15-30 minutes. d. Store the frozen culture under vacuum conditions in powdered trehalose sugar at a concentration of 15.53-18.81%. Weight per volume was measured by storing each bacterial strain separately at temperatures ranging from -50 to -30 degrees Celsius. The pressure of the instrument is adjusted to be in the range of 0.075-0.150 millibars. Mixing powdered probiotic cultures. J. Mix the powdered probiotic culture from both bacterial strains under sterile conditions using a mixer. At a speed of 40-60 revolutions per minute, for 20-40 minutes, add the Lactobacillus reuteri powder. (Lactobacillus reuteri TISTR2736) into the mixer, followed by Lactobacillus saei powder (Lactobacillus zeae TISTR2529) f. Pack the thoroughly mixed powdered probiotic culture into vacuum-sealed foil bags. Immediately, ensure the net weight is between 50-200 grams per bag, ready for packaging.