Elephant interferon gamma test kit and procedure for measuring elephant interferon gamma in plasma using the kit.
Patent Information
- Authority / Receiving Office
- TH · TH
- Patent Type
- Utility models
- Current Assignee / Owner
- Filing Date
- 2022-05-06
- Publication Date
- 2026-03-23
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Abstract
Claims
OCR 09WP 16 / 03 / 2569 The kit for detecting interferon gamma in elephants using monoclonal antibodies includes the following solutions: Phosphate buffer saline, blocking solution (PBS + 10% Fetal Bovine Serum), washing solution, antibody binding. The detectable antibody, substrate (TMB substrate), and reaction stopper have specific characteristics. Antibodies that bind can be selected from mouse monoclonal antibodies against recombinant interferon. Ferron gamma elephant (mouse anti-relFN gamma monoclonal antibody) derived from hybridoma clone code. F1C3#17 Isotype IgG2b The detectable antibody consists of two types of antibodies: primary antibodies that can be selected from the molar antibody. Mouse anti-chlorochloride antibodies against recombinant interferon gamma of elephant (mouse anti- relFN (gamma monoclonal antibody) derived from hybridoma clone F1C3#30, isotype IgG1. And secondary antibodies selected from rat antibodies against immunoglobulin isotype IgG1 of... Rat antimouse labeled with HRP enzyme (rat antimouse IgG1-HRP) 2. Procedure for detecting interferon gamma in elephants using an elephant interferon gamma detection kit. Under Claim 1, the following steps apply: a) Place the cell / or plasma culture medium of the protein-stimulated sample into a layered container. Mouse antibodies against recombinant elephant interferon gamma (mononclonal antibodies from...) The hybridoma (clone code F1C3#17) was then incubated at 4°C for 14-18 hours. After an hour, rinse with detergent three times. b) Add a monoclonal antibody from hybridoma clone F1C3#30 and incubate at 37°C. For 60 minutes, rinse three times with the cleansing solution. c) Add rat antibodies against rat isotype IgG1 immunoglobulin labeled with HRP enzyme. Diluted with blocking solution, cure at 37°C for 30 minutes, then rinse with cleaning solution 3. time d) Add the TMB substrate solution, incubate in the dark for 5 minutes, and add the reaction stopper. c) Measure the absorbance at a wavelength of 450 nanometers using a microplate reader.
3. Procedure for detecting interferon gamma in elephants using an elephant interferon gamma detection kit, as per point... The second right is held in which the protein-activated sample is obtained by separating plasma from whole blood. (Whole blood) stimulated by the poke weed mitogen, PPD-B, PP. PPD-A and tuberculosis-specific antigens were incubated at 37°C under a controlled atmosphere. 5 percent carbon dioxide reduction for 24 hours.
4. Procedure for detecting interferon gamma in elephants using an elephant interferon gamma detection kit, as per point... The third right is held where the tuberculosis-specific antigen can be selected from ESAT6, CFP10, or Rv3020c. Either one or a combination of several.
5. Procedure for detecting interferon gamma in elephants using an elephant interferon gamma detection kit, as per point... The second right is held by a class of mouse antibodies against recombinant elephant interferon gamma (monoclonal). The antibody from the hybridoma (clone code F1C3#17) is prepared using the following steps: a) Immobilize the monoclonal antibody from the hybridoma, clone F1C3#17, on a 96-well dish. Maxisorb at a dilution rate of 5 micrograms per milliliter with PBS at a volume of... 100 microliters, incubated at 37 degrees Celsius for 60 minutes, washed with washing solution, 200 ml per well. Microliters, a total of 3 times. b) Add 200 microliters of block solution (PBS + 10% Fetal Bovine Serum) to each well and incubate at... Leave at room temperature for 60 minutes. Rinse three times with detergent.