Formulation and production process of exopolysaccharide from broken riceberry rice and soybean meal.

Abstract

OCR 10KL (16 / 02 / 2569) Formulation and production process of exopolysaccharide from broken riceberry rice and... Soybean meal consists of broken riceberry powder, soybean meal, and bacilli starter culture, adjusted in volume with distilled water. It consists of the following steps: Grinding broken riceberry rice and soybean meal into powder; then taking the broken riceberry rice and soybean meal powder... The yellow liquid is mixed with distilled water and sterilized to obtain a liquid culture medium. Bacillus inoculum is added, and the mixture is incubated at 25°C. At 45°C, shake at 100-200 rpm. Precipitate the exopolysaccharide by adding ethanol. Then, soak it overnight, and centrifuge to separate the exopolysaccharide precipitate. Take the obtained exopolysaccharide and... Drying at temperatures of 25-50 degrees Celsius yields exopolysaccharide extracts that are used as food additives. Animals are used to combat pathogens in tilapia, thereby reducing farmers' costs associated with using antibiotics in tilapia farming.

Claims

OCR 10KL (16 / 02 / 2569) 1. The exopolysaccharide formula from broken riceberry rice and soybean meal consists of: A. Broken riceberry rice powder, in a proportion of 4-10 percent by weight to volume (W% / V). B. Soybean meal, 1-10 percent by weight to volume (W% / V). C. Bacillus inoculum in a ratio of 1-10 percent by volume to volume (V% / V). D. Distilled water. Adjust the volume to 100 percent by volume.

2. Method for producing exopolysaccharide from broken riceberry rice and soybean meal. The process involves the following steps: a) Grind the broken riceberry rice and soybean meal into a powder. b) Add 4-10 percent by weight / volume (W% / V) of broken riceberry rice powder and soybean meal. Yellow dye, in a proportion of 1-10 percent by weight to volume (W / V), is mixed in distilled water and used for sterilization, resulting in... Liquid culture medium c) Add Bacillus inoculum in a ratio of 1 - 10 percent by volume to volume (V% / V) to obtain... The final volume is 100 percent by volume. Pour it into the liquid culture medium from step b) and incubate it at... Maintain a temperature of 25-45 degrees Celsius, shaking at 100-200 revolutions per minute for 24-72 hours. d) Precipitate the exopolysaccharide by adding ethanol to the solution from step c) and then immerse it. Leave it overnight at a temperature of 4-25 degrees Celsius. c) Centrifugation is performed to separate the exopolysaccharide precipitate. f) Dry the exopolysaccharides obtained from step c) at a temperature of 25-50 degrees Celsius. Until the dry weight is reduced to 3.00-4.00 grams, a creamy white solid exopolysaccharide extract is obtained.

3. Method for producing exopolysaccharide from broken riceberry rice and soybean meal. Under Claim 2, whereby step b) involves sterilization with steam pressure between 15–20 pounds per square inch and The temperature will be between 110 and 122 degrees Celsius for 15 to 20 minutes.

4. Method for producing exopolysaccharide from broken riceberry rice and soybean meal. Under Claim 2, Step d) provides for the addition of ethanol in twice the volume of the liquid culture medium.