A method for detecting latent group B streptococcus infection in pregnant women using real-time polymerase chain reaction (PCR) technique.

Abstract

Method for detecting latent group B streptococcus infection in women. Pregnancy can be achieved using real-time polymerase chain reaction (PCR) technology, which includes a primer and... A probe specific to the cfb gene of Streptococcus beta-positive bacteria for use in detection. Effective diagnosis allows for the specific identification of Group B Streptococcus bacteria, leading to... It can treat and prevent infections in newborns and reduce the incidence of infant mortality due to infection. Streptococcus group B

Claims

1. Methods for detecting latent group B streptococcus infection in pregnant women. Real-time polymerase chain reaction (PCR) technique for detecting the cfb gene in Streptococcus beta-positive bacteria. Specifically, it contains primers for adding specific genetic material. Two strands of Streptococcus beta-fluid with specific characteristics: A forward primer consisting of the following base sequence: 5'-ACG TTA CAC ACA TGA TGT GTC TAT CTG G-3' A reverse primer consisting of the following base sequence: 5'-ACA TCT GTT AAG GCT TCT ACA CGA CTA CC-3' 2. Methods for detecting latent group B streptococcus infection in pregnant women. Real-time polymerase chain reaction (PCR) technique, according to claim 1, where genetic material is added using a DNA set. The prototype works in conjunction with a detector or probe for measuring the quantity of genetic material using fluorescent substances. The light is composed of the following base sequence: 5'-FAM-CAA GCT TTT GAG CCA TTT GCT GGG CTT G-3'-BHQ1 3. Methods for detecting latent group B streptococcus infection in pregnant women. Real-time polymerase chain reaction (PCC) technique under Claim 1, where programmable temperature settings for Increase the amount of genetic material as follows: -Step 1: Heat to 95 degrees Celsius for 20 seconds. - Step 2: Heat to 95 degrees Celsius for 3 seconds. - Step 3: Maintain a temperature of 57 degrees Celsius for 30 seconds (repeat steps 2 and 3 50 times). - Step 4: Maintain a temperature of 72 degrees Celsius for 5 minutes.

4. Methods for detecting latent group B streptococcus infection in pregnant women. Real-time polymerase chain reaction (PCR) technique, under claim 1, where samples are used for pathogen detection. Streptococcus group B can be detected from vaginal, cervical, rectal, or urine samples.