A biological synthesis method for gold nanoparticles using silk sericin protein extract and UV irradiation techniques for biomedical applications.

Abstract

OCR 10KL (07 / 11 / 2568) This invention reveals a method for biosynthesizing gold nanoparticles using gold compounds. Chloride trihydrate (HAuCl4.3H2O) was used as a starting material, and the protein extract from silk sericin was used as a substrate. It is a reducing agent and stabilizes gold nanoparticles in coil suspension systems. The use of the extract... To avoid the toxicity associated with synthetic silk protein, the silk fibers are used without the use of potentially harmful chemicals. It is harmful to humans and the environment, and contains a mixture ratio of gold chloride trihydrate and silk sericin. In equal ratios, UVC light is used to induce the gold nanoparticle formation reaction. The synthesized gold nanoparticles were found to change the color of the colloid from yellow to wine red, and had a diameter of... At the center of the 5-15 nm particles, the volume of the gold nanoparticles increases, and the distribution becomes higher. When the duration of UV radiation exposure was increased, no toxicity was observed in mouse muscle cells.

Claims

OCR 10KL (07 / 11 / 2568) 1. A method for synthesizing gold nanoparticles encapsulated in silk sericin using UV irradiation. The process is as follows: A. Prepare the silk sericin protein extract by starting with cutting the silkworm cocoon (Bombyx mori) into pieces and boiling it. Five grams of cut silkworm cocoons were treated with a 0.5% sodium carbonate (Na2CO3) solution. By mass per volume, at a temperature of 85 degrees Celsius for 45 minutes. B. Take the silk gum protein solution extracted in step A and remove the salts and silk sericin molecules. The unwanted size particles are removed from the resulting solution by placing the solution into a dialysis bag. A dialysis bag with a pore size of 3,500 Daltons (Molecular weight cut off 3,500 Da) will... They were immersed in deionized water for 2-3 days, with the deionized water being changed periodically. Every 12 hours C. Concentrate the dialysis bag containing the silk sericin solution from step B by immersing the dialysis bag in... A polyethylene glycol solution (PEG-6000) with a concentration of 10-20% by mass per volume. For 24 hours. D. Use the concentrated silk sericin solution obtained from step C to remove fats and insoluble residues. During the extraction process using a centrifuge at a speed of 3,500 revolutions per minute. At a temperature of 25 degrees Celsius for 20 minutes. D. Dry the silk sericin solution obtained from step D into a powder using the freeze-drying method. (or lyophilization) for 24 hours. f. Prepare a silk sericin solution with a concentration of 1-2% by mass / volume by taking the powder... The silk sericin obtained from step E is dissolved in pure distilled water. B. Prepare a gold chloride trihydrate (HAuCl4.3H2O) solution with a concentration of 1-3 millimolar. B. Adjust the pH of the gold chloride trihydrate solution prepared in step C. The pH can be adjusted to 7-10 by adding a concentrated sodium hydroxide (NaOH) solution. Dilute to prevent the aggregation of silk sericin. J. Mix the solutions obtained from steps F and H in equal proportions (1:1). J. Irradiate the mixed solution obtained from step N with UV-C light having a wavelength of 200-280 nanometers. At temperatures of 25-30 degrees Celsius for 1-15 hours, a colloidal substance of nanoparticles is formed. Gold with a wine red color D. The colloidal material of gold nanoparticles obtained from step D was then freeze-dried. After drying or lyophilization for 24 hours, gold nanoparticles encapsulated in strands are obtained. Silk Sericin 2. Synthesis method for gold nanoparticles encapsulated in silk sericin using UV irradiation technique. According to claim 1, the component containing the silk sericin protein extract can form bonds. Sulfide on the surface of gold nanoparticles.

3. Synthesis method for gold nanoparticles encapsulated in silk sericin using UV irradiation technique. According to claim 1 or 2, gold nanoparticles have a size of 5-15 nanometers.

4. Synthesis method for gold nanoparticles encapsulated in silk sericin using UV irradiation technique. Under any one of claims 1 through 3, gold nanoparticles encapsulated in silk sericin strands interact. The covalent bond formed by the thiol (-SH) group of the amino acid within the silk sericin molecule.

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