The gene expression sequence is controlled by a continuously expressing promoter, glyceroldehyde triphosphate dehydrogenase for Aspergillus, and a recombinant plasmid containing this gene.

Abstract

OCR 09WP 02 / 12 / 2567 The gene expression sequence is controlled by a glycerol-mediated, continuous-expression promoter. Dehyde triphosphate dehydrogenase consists of the anterior region of the glyceroldehyde triphosphate dehydrogenase gene. A Geniscential element containing the Start Decoding Region (TSS), at least one TATA box, and CCAAT. At least one box loci and at least one GC box loci, in the glyceraldehyde triphosphate gene segment. Dehydrogenase and the posterior TrpC terminator of the gene, which is a set of gene expressions under promo control. Such continuously expressing filamentous fungi can be used to develop more promising filamentous fungal strains. In the production of targeted bioactive substances that are of interest and in demand by the industry, as well as for use in functional studies. The gene of interest, which is under the control of this promoter, will be expressed continuously throughout the period. Cells grow without the need for gene expression induction agents, which reduces costs and The complexity of the microbial culturing process in producing the target substance.

Claims

OCR 09WP 02 / 12 / 2567 1. Gene expression patterns under the control of the glyceraldehyde constant-expression promoter. Triphosphate dehydrogenase for Aspergillus consists of: The anterior region of the glyceroldehyde triphosphate dehydrogenase gene contains components of the region. Start decoding (TSS), at least one TATA box, at least one CCAAT box. and at least one GC box loci, the gene segments of glyceroldehyde triphosphate dehydrogenase and tert... The TrpC minators are arranged in order from the 5' to the 3' side.

2. Gene expression patterns under the control of a glyceraldehyde-mediated, continuous-expression promoter. Triphosphate dehydrogenase for Aspergillis, according to claim 1, where the anterior region of the gene... The glyceroldehyde triphosphate dehydrogenase in question has a nucleotide sequence length of 490 to 2400 nucleotides. Tide 3. Gene expression sequences under the control of a glyceraldehyde-mediated, continuous-expression promoter. Triphosphate dehydrogenase for Aspergillis, according to claim 2, where the nucleotide sequence from the region The anterior portion of the glyceroldehyde triphosphate dehydrogenase gene can be selected from fungi in the Aspergilli group. Mucor, Beauveria, Penicillium (one of them) 4. Gene expression sequences under the control of a glyceraldehyde-mediated, continuous-expression promoter. Triphosphate dehydrogenase for Aspergilli, according to claim 3, where Aspergilli fungi have selective choice. From Aspergillus oryzae, Aspergillus nidulans, Aspergillus niger, the appropriate one is Aspergillus. oryzae 5. Gene expression sequences under the control of a glyceraldehyde-mediated, continuous-expression promoter. Triphosphate dehydrogenase for Aspergillus pursuant to claim 1, where the transcription initiation site is located. The (TSS) region is the -926 to -986 nucleotide region prior to the translation start (+1 nucleotide). Glyceroldehyde triphosphate dehydrogenase 6. Gene expression sequences under the control of a glyceraldehyde-mediated, continuous-expression promoter. Triphosphate dehydrogenase for Aspergillus pursuant to claim 1, as stated in the TATA box and CCAAT box. And the GC box has the following characteristics: The TATA box 1 position mentioned is at position -540, the nucleotide preceding the translation start point. (+1 nucleotide) glyceroldehyde triphosphate dehydrogenase CCAAT box 2, specifically positions -2073 and -2158, are the nucleotide positions to check first. Translation start point (+1 nucleotide): glyceroldehyde triphosphate dehydrogenase The GC box position mentioned is -2184, the pre-translational nucleotide position. (+1 nucleotide) glyceroldehyde triphosphate dehydrogenase 7. Recombinant plasmids containing a set of gene expression genes under the control of a continuously expressing promoter. Glyceroldehyde triphosphate dehydrogenase type for Aspergillus fungus and gene expression set. Selected as a plasmid containing the UidA reporting gene, under promo control. The continuously expressed terminator PGpdA, the terminator TrpC, and the expression set of related genes. Creating nutrients for selecting transformative fungi.

8. Recombinant plasmids containing a set of gene expression genes under the control of a continuously expressing promoter. Glyceroldehyde triphosphate dehydrogenase type for Aspergillus fungus and gene expression set. Selection is based on claim 7, where the gene expression set involved in nutrient production for… The selected transformants can be from any one of the following genes: PyrG, NiaD, AdeA, or ArgB. One suitable gene is the PyrG gene.

9. Recombinant plasmids containing a set of gene expression genes under the control of a continuously expressing promoter. Glyceroldehyde triphosphate dehydrogenase type for Aspergillus fungus and gene expression set. Selection is based on claim 8, which states that the PyrG gene can be selected from filamentous fungi of the Aspergilli group or yeast. Either one

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