The process of manufacturing nanoparticles for delivering antioxidants.

Abstract

OCR 09WP 01 / 10 / 2568 Nanoparticles for delivering antioxidants have a core-shell structure. It has three main components: the core component is the antioxidant quercetin (QU). The shell consists of polymers in the polyester chain group, namely polyvinyl alcohol (Poly(lactic-co- The components include glycolic acid (PLGA) and a surfactant that helps stabilize the nanoparticles, namely polyvinyl oxide. Polyvinyl alcohol is used in the production of nanoparticles for delivering antioxidants. The process involves several steps. Dissolve the polymer and antioxidant in the solvent. Mix the solution drop by drop until homogeneous. Using high-frequency sound waves, prepare a surfactant solution in ionizing water. Add the mixed solution to the surfactant. Stir the surface until the solvent evaporates, then centrifuge to separate the precipitate and disperse the particles in water into a colloidal substance. Freeze it, then freeze-dry it, and store it for later use.

Claims

OCR 09WP 01 / 10 / 2568 1. Nanoparticles for delivering antioxidants, which consist of: The shell accounts for 60 to 65 percent by weight. The core content is 35 to 40 percent by weight. Where the outer shell is made of poly(lactic-co-glycolic acid, PLGA). Molecular weight range 24-38 kilodaltons (kDa). The core ingredient is the antioxidant active ingredient quercetin (QU). They have a size range of 200 - 300 nanometers, a pH range of 4.1 - 5.6, and a surface charge. For particles in the range of -30 to -20 millivolts.

2. The process for manufacturing nanoparticles for the delivery of antioxidants, as per claim 1, which includes: Follow these steps: A. Prepare the poly(lactic-co-glycolic acid) (PLGA) precursor. A concentration of 1.5-2.0% by mass per volume is achieved by adding poly(lactic-co-glycolic acid) by weight. The molecules, in the range of 24-38 kilodaltons (kDa), are soluble in the solvent dichloromethane. DCM) for the synthesis of quercetin (QU)-poly(lactic-co-glyco) nanoparticles. (QU-PLGA) B. Prepare the antioxidant active ingredient quercetin (QU) at a concentration of 20.0-25.0% by mass. By volume, quercetin is dissolved in dimethyl sulfoxide solvent. DMSO) C. Add the active ingredient solution from step B to the poly(lactic-co-glycolic acid) starting material solution. (PLGA) As in point A, drop by drop. D. Take the solution mixture from step C and subject it to a homogenization process using a sound wave generator. A high-frequency probe sonicator with a 3 mm probe diameter and a power of 130 watts. Watts, frequency 20 kilohertz (kHz) for 1 minute, using amplitude 20-30%. Or until each component of the mixed solution is homogeneous. C. Prepare polyvinyl alcohol (PVA) solutions with concentrations of 3.0-5.0% by mass per volume. Thus, weigh polyvinyl alcohol (PVA), which has a molecular weight in the range of 31-50 kilodaltons, and dissolve it in water to remove [the chemical residue / degradation effect]. The deionized water is stirred and heated to 100 degrees Celsius until polyions are formed. The polyvinyl alcohol (PVA) solution is completely dissolved. Allow the polyvinyl alcohol (PVA) solution to cool. The polyvinyl alcohol (PVA) solution was heated to room temperature and then filtered using a 0.2 micron pore filter. micrometer F. Add the mixture from step D to the polyvinyl alcohol (PVA) solution until it reaches the desired consistency. A concentration of 31% by volume was added drop by drop, controlling the drip rate to be within the range of 1. milliliters / minute B. Take the solution mixed in step F and subject it to a process for homogenization and particle size reduction. A probe-type sonicator with a probe diameter of 13. Millimeters, 750 watts of power, a frequency of 20 kilohertz (kHz) for 5 minutes, using amplitude... (amplitude) 20-40% C. Stir the solution with a stirrer at room temperature for another 5 hours, or until the organic solvent is dissolved. It evaporated completely. J. The resulting nanoparticle solution is centrifuged to separate the nanoparticle precipitate from the solution. Using a centrifuge at a speed of 15,000 revolutions per minute at a temperature of 4 degrees Celsius. Celsius for 30 minutes. B. Remove the solution from the centrifugation and add deionized water in the following proportion: The volume ratio (milliliters) is 2.5 to 1 to disperse the new nanoparticles into a colloidal substance. The colloidal nanoparticles were then stored at -80 degrees Celsius for 24 hours. D. The solidified colloidal material is then dried using the freeze-drying method. After dehydration or lyophilization for 24 hours, powdered nanoparticles were obtained.

3. The process for manufacturing nanoparticles for the delivery of antioxidants, as per claim 2, which includes: With the following more appropriate steps: A. Prepare the poly(lactic-co-glycolic acid) (PLGA) precursor. A concentration of 1.7 grams per milliliter was achieved by using poly(lactic-co-glycolic acid) with a molecular weight range... 24-38 kilodaltons (kDa) are soluble in dichloromethane (DCM) solvent. For the synthesis of quercetin-poly(lactic-co-glycolic acid) (QU-PLGA) nanoparticles. B. Prepare a solution of the active antioxidant quercetin at a concentration of 25 grams per milliliter. Quercetin is dissolved in dimethyl sulfoxide (DMSO) solvent. C. Add the active ingredient solution from step B to the reactant solution (lactic-co-glycolic acid). (PLGA) As in point A, drop by drop. D. Take the solution mixture from step C and subject it to a homogenization process using a sound wave generator. A high-frequency probe sonicator with a 3 mm probe diameter and a power of 130 watts. Watts, frequency 20 kilohertz (kHz) for 1 minute, using an amplitude of 30%. C. Prepare a 5.0% by mass / volume polyvinyl alcohol (PVA) solution as follows: Weigh 2.5 grams of polyvinyl alcohol (PVA), with a molecular weight in the range of 31-50 kilodaltons, and dissolve it. In 50 ml of deionized water, stir while heating. Heat at 100 degrees Celsius until the polyvinyl alcohol (PVA) is completely dissolved. Let the polyvinyl solution rest. Cool the polyvinyl alcohol (PVA) to room temperature, then filter the polyvinyl alcohol (PVA) solution. By using a filter with pores measuring 0.2 micrometers. F. Add the mixture from step D to the polyvinyl alcohol (PVA) solution until it reaches the desired consistency. A concentration of 31% by volume was added drop by drop, controlling the drip rate to be within the range of 1. milliliters / minute B. Take the solution mixed in step F and subject it to a process for homogenization and particle size reduction. A probe-type sonicator with a probe diameter of 13. Millimeters, 750 watts of power, a frequency of 20 kilohertz (kHz) for 5 minutes, using amplitude... (amplitude) 40% C. Stir the solution with a stirrer at room temperature for another 5 hours, or until the organic solvent is dissolved. It evaporated completely. J. The resulting nanoparticle solution is centrifuged to separate the nanoparticle precipitate from the solution. Using a centrifuge at a speed of 15,000 revolutions per minute at a temperature of 4 degrees Celsius. Celsius for 30 minutes. B. Remove the solution from the centrifugation and add deionized water in the following proportion: The volume ratio (milliliters) is 2.5 to 1 to disperse the new nanoparticles into a colloidal substance. The colloidal nanoparticles were then stored at -80 degrees Celsius for 24 hours. D. The solidified colloidal nanoparticles were then dried using the freeze-drying method. After dehydration or lyophilization for 24 hours, powdered nanoparticles were obtained.

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