A method for preparing a serum product containing deer antler velvet extract encapsulated in positively charged liposome particles combined with micron-sized needles.

Abstract

OCR 10KL (05 / 01 / 2569) The process for preparing a serum product containing deer antler velvet extract encapsulated in positively charged liposome particles using a needle. Micron size: The process involves preparing liposome particles from phospholipids and cholesterol, followed by addition... Deer antler velvet extract and polysorbate 20 were centrifuged to reduce the particle size of the liposomes using a probe-type ultrasonic machine. Excess fat was separated from the formula using a centrifuge, and the encapsulated deer antler velvet extract was then used. Liposome particles are mixed with a chitosan solution and then packed into a serum formulation containing hydroxypropyl. Methyl cellulose (HPMC), Microcare Registered Trademark PHC, and ethylenediamine tetraacetic acid disodium are then added. Micron-sized needles are used to obtain deer antler velvet extract encapsulated in positively charged liposome particles along with micron-sized needles. micron

Claims

OCR 10KL (20 / 04 / 2569) 1. Preparation method for deer antler velvet extract serum encapsulated in positively charged liposomes using a needle. Micron size measurement consists of the following steps: a. Prepare liposome particles from phospholipids and cholesterol at a specific ratio between phospholipids and cholesterol. The cholesterol ratio is 10:2 millimolar, dissolved in chloroform and ethanol, and then... Dry by evaporation using nitrogen gas and then storing in a desiccant jar for 6 hours to form a lipid film. of liposomes B. Dissolve deer antler velvet extract at a concentration of 0.1 - 0.4 %w / w in distilled water and add it to the film. Liposome lipids C. Add polysorbate 20 at a concentration of 1.0 - 4.0 %w / w to the solution. liposomes d. The liposome particles are reduced in size by centrifugation using ultrasonic probe systems. For 10-60 minutes at 120-150 watts and 30-80 kilohertz (kHz). J. Separate the excess fat from the recipe using a centrifuge at a specific speed. 15,000 rpm at 4°C for 1-30 minutes. F. Blend the liposome-encapsulated deer antler velvet extract with a chitosan solution (concentration 10). The ratio between the deer antler velvet extract encapsulated in liposomes and the chitosan solution is: 10:1 - 10 (ideal is 10:1) for 30 minutes - 2 hours. B. The deer antler velvet extract, encapsulated in positively charged liposome particles, is then incorporated into a serum formulation. Contains 2.0% w / w hydroxypropyl methyl cellulose (HPMC), phenoxyethanol (and) Chlorphenesin (and) Aqua (and) Glycerin (Phenoxyethanol (and) Chlorphenesin (and) Aqua (and) Glycerin) 0.8% w / w, and ethylenediamine tetraacetic acid disodium. 0.05% w / w C. Micron-sized needles containing sponge extract at concentrations of 0.1 - 2 % w / w were added to deer antler extract serum. weak At this stage, you will receive a serum product containing deer antler velvet extract encapsulated in positively charged liposome particles, along with a small needle. Place the micron sample in a dry, clean container and close the lid tightly.

2. Preparation method for deer antler velvet extract serum encapsulated in positively charged liposomes using a needle. Micron size, according to claim 1, where the optimal conditions are to prepare liposome particles from phospholipids and... Cholesterol was then treated with a 0.2% w / w concentration of deer antler velvet extract and polysorbate 20%. A 2.0% w / w concentration was used to reduce the particle size of the liposomes using a probe-type ultrasonic centrifuge for 30 minutes. At 130 minutes, 130 watts, and 40 kilohertz (KHz), the machine separates excess fat from the recipe. Centrifugation is performed for 15 minutes, after which the deer antler velvet extract encapsulated in liposomes is mixed with... The chitosan solution was steeped in a 10:1 ratio for 1 hour, after which the collected deer antler velvet extract was applied. Positively charged liposome particles are encapsulated in the serum formulation, and a micron-sized needle is added at a concentration of 1. %w / w