A method for rapid assessment of total carotenoid content in the algae Scenedesmus amatus using UV-visible spectrophotometry.

Abstract

Methods for evaluating total carotenoid content in the algae *Sinedesmus*. (Scenedesmus amatus) rapidly using UV-visible spectrophotometry. Spectrophotometry) begins with cleaning the cells of the algae Scenedesmus amatus. Next, drops of Scenedesmus amatus algae cells are placed on an absorbent material, and the light absorption values ​​are measured. Using a UV-visible spectrophotometer, a sample detection unit was installed. A remote diffuse reflectance accessory (RDRA) is used to measure the absorbance values. The measured amount provides an estimate of the total carotenoid content.

Claims

1. Method for evaluating total carotenoid content in the algae *Sinedesmus*. (Scenedesmus amatus) rapidly using UV-visible spectrophotometry. The spectrophotometry process involves the following steps: a. Clean the Scenedesmus amatus algae cells by pipetting the algal solution. A sample containing 120 x 10⁶ algae cells was placed in a tube with a volume of 1 milliliter and then centrifuged. (Centrifuge) at 4,000 revolutions per minute for 5 minutes. Pour off the liquid to obtain algal cell sediment. Then add 2 milliliters of MilliQ water, centrifuge, and discard the liquid. Repeat this process twice to obtain a precipitate. The algal shells are washed and cleaned, and the resulting algal cell sediment is then dissolved by adding MilliQ water. Add 300 microliters and mix to ensure the algal cells are evenly distributed in the solution. B. Pipette 5 microliters of the solution prepared in step A onto the absorbent material, then proceed as follows: Dry at room temperature in the dark for 30 minutes to obtain frozen Scenedesmus amatus algae. It is located on the absorbent material. C. Take the Scenedesmus amatus algae that are fixed on the absorbent material obtained from step B. Let's analyze the total carotenoid content using a UV-visible spectrophotometer. A visible spectrometer) is installed, along with a remote diffuse spectrometer for detecting solid samples. Reflectance Accessory (RDRA) and UV-visible spectrophotometer. And the absorbance values ​​at wavelengths in the range of 250-750 nanometers are used. d. Use the absorbance value equivalent to light transmission at a distance of 10 millimeters at 480 nanometers (A48o) obtained from step c. Let's estimate the total carotenoid content using Equation 1. Total carotenoid content (micrograms per milliliter) = 4 x A480 Equation 1 Where A480 = absorbance value equivalent to light transmission over a distance of 10 millimeters at 480 nanometers = 10xA*. A* = The absorbance value at a given wavelength measured by a solid-type sample detector. Remote Diffuse Reilectance Accessory (RDRA)

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