The formula for a starter culture used in the decomposition of organic fertilizer, and the manufacturing process of that formula.

Abstract

------16 / 03 / 2569------(OCR) OCR 09WP This invention describes a starter culture formula for organic fertilizer decomposition. It's a prototype microbial starter culture developed to reduce production costs and improve yield efficiency. The formula contains at least five types of microorganisms: Aspergillus sp., Streptomyces sp., Brevibacillus sp., Paenibacillus sp., and Lactobacillus sp., along with other compounds such as bentonite, dolomite, or modified starch. The production process involves isolating and multiplying the desired microorganisms. The resulting fermented liquid containing microbial cells is then sprayed onto a carrier and dried without heat. The resulting powdered microbial cultures are then mixed according to the starter culture formula. The quality and quantity of the microbial starter culture are then monitored and controlled.

Claims

------16 / 03 / 2569------(OCR) OCR 09WP1. A starter culture formula for organic fertilizer decomposition containing the following components: a. Aspergillus sp. 5-10% by weight / volume b. Streptomyces sp. 5-10% by weight / volume c. Brevibacillus sp. 5-10% by weight / volume d. Paenibacillus sp. 5-10% by weight / volume e. Lactobacillus sp. 5-10% by weight / volume f. Maltodextrin 1-10% by weight / volume g. A selectable compound from bentonite, dolomite, or modified starch, 40-60% by weight / volume.

2. Starter culture formula for organic fertilizer decomposition. According to claim 1, Aspergillus sp. is selectable from Aspergillus niger, Aspergillus nidulans, and Aspergillus oryzae, either individually or in combination; Streptomyces sp. is selectable from Streptomyces puniceus, Streptomyces griseus, and Streptomyces celicolor, either individually or in combination; and Brevibacillus sp. is selectable from Brevibacillus reuszeri, Brevibacillus brevis, and Brevibacillus laterosporus.One or more combinations of Paenibacillus sp. can be selected from Paenibacillus jamilae, Paenibacillus polymyxa, and Paenibacillus macerans, either individually or in combination. Lactobacillus sp. can be selected from Lactobacillus rhamnosus, Lactobacillus acidophilus, and Lactobacillus plantarum, either individually or in combination.

3. Inoculum formulations for use in the decomposition of organic fertilizers according to claim 2, where the optimal formulation is: a. Aspergillus sp. 5-10% by weight / volume b. Streptomyces puniceus 5-10% by weight / volume c. Brevibacillus reuszeri 5-10% by weight / volume d. Paenibacillus jamilae 5-10% by weight / volume e. Lactobacillus rhamnosus 5-10% by weight / volume f. ... Maltodextrin 1-10% by weight / volume. A selectable compound of bentonite, dolomite, or modified starch, 40-60% by weight / volume.

4. A starter formulation for use in the decomposition of organic fertilizers according to one of Patents 1-3.Where additional components can be selected from ammonium dihydrogen phosphate or molasses, either individually or in combination.

5. A starter culture formulation for organic fertilizer decomposition according to Patent 4, where the appropriate ammonium dihydrogen phosphate is in the range of 2-10% by weight / volume, and the appropriate molasses is in the range of 0.5-2% by weight / volume.

6. A starter culture formulation for organic fertilizer decomposition according to any one of Patents 1-5, where maltodextrin, bentonite, dolomite, ammonium dihydrogen phosphate, modified starch, and molasses act as carriers.

7. A process for producing a starter culture formulation for organic fertilizer decomposition according to any one of Patents 1-6, which is done by isolating the desired microorganisms, then culturing the isolated microorganisms in a culture medium. For bacteria, the number of microorganisms in the fermentation tank is increased to 10⁷ - 10¹⁶ CFU / mL, while for fungi... Increase the number of fungal spores in solid media using grain seeds, of which some can be selected.Rice, sorghum, and maize, either individually or in combination, are used to increase the number of fungal spores to 10⁷-10¹⁶ spores per gram. The resulting weight of microbial cells and spores is then sprayed onto a carrier medium. The contamination rate with other microorganisms must be within the range of 0-1 percent. The sprayed carrier medium is then dried without heat. The resulting powdered microbial culture is then mixed according to the specified inoculum formula. This process includes additional steps for monitoring and controlling the quantity / quality of the microbial culture by counting the number of each type of microbial culture after formulation, storing it for 12 months, and regularly counting the number of surviving cultures monthly.

8. The process of formulating the inoculum for use in organic fertilizer decomposition, as per claim 7, involves drying the microorganisms without heat using a fluidized bed dryer, sun-drying, or vacuum drying, either individually or in combination.