A method for culturing turmeric germinated tissue without surface sterilization.

Abstract

A plant tissue culture method that does not require bleaching, but instead uses a tissue culture medium. MS (Multimediated Soy Sauce) formulas contain sodium hypochlorite or chlorine dioxide immediately, without the need to let the food sit. The process begins with preparing tissue from turmeric sprouts grown from mushrooms. The turmeric sprouts are then cleaned. Remove all soil and impurities, then cultivate them in a tissue culture medium containing sodium hypochlorite. Disinfect the turmeric sprouts with chloride or chlorine dioxide and immediately place the cleaned turmeric sprouts on the food. To ensure that cultured tissues are sterile without the need for sterilization processes.

Claims

OCR 09WP 18 / 05 / 2569 1. The process of culturing turmeric germinated tissue without surface sterilization involves the following steps: A. Prepare plant tissue culture medium (MS) formula supplemented with growth regulators. N6-Benzyladenine (BA) at a concentration of 2 mg / L, in combination with... Sodium hypochlorite at a concentration of 0.006 - 0.03% or chlorine dioxide at a concentration of 0 - 50 ppm. By volume, in either case, add 30 grams of granulated sugar per liter to all recipes and adjust the pH. Make it 5.6 - 5.

8. B. Wash the turmeric sprouts thoroughly, remove the leaves, and then cultivate them in a growth medium. For plant tissue culture, proceed immediately from step A and cultivate it at a temperature of 25 ± 2 degrees Celsius. Provide light for 16-18 hours per day for 8 weeks, replacing the food with freshly prepared food using the following method: Same as option A, every 4 weeks.